Preliminary Phytochemical and Antimycobacterial Investigation of Some Selected Medicinal Plants of Endau Rompin, Johor, Malaysia
Total Page:16
File Type:pdf, Size:1020Kb
Journal of Science and Technology, Vol. 10 No. 2 (2018) p. 30-37 Preliminary Phytochemical and Antimycobacterial Investigation of Some Selected Medicinal Plants of Endau Rompin, Johor, Malaysia Shuaibu Babaji Sanusi*, Mohd Fadzelly Abu Bakar, Maryati Mohamed and Siti Fatimah Sabran Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (UTHM), Pagoh Educational Hub, 84600 Pagoh, Johor, Malaysia. Received 30 September 2017; accepted 27 February 2018; available online 1 August 2018 DOI: https://10.30880/jst.2018.10.02.005 Abstract: Tuberculosis (TB), the primary cause of morbidity and mortality globally is a great public health challenge especially in developing countries of Africa and Asia. Existing TB treatment involves multiple therapies and requires long duration leading to poor patient compliance. The local people of Kampung Peta, Endau Rompin claimed that local preparations of some plants are used in a TB symptoms treatment. Hence, there is need to validate the claim scientifically. Thus, the present study was designed to investigate the in vitro anti-mycobacterial properties and to screen the phytochemicals present in the extracts qualitatively. The medicinal plants were extracted using decoction and successive maceration. The disc diffusion assay was used to evaluate the anti-mycobacterial activity, and the extracts were subjected to qualitative phytochemical screening using standard chemical tests. The findings revealed that at 100 mg/ml concentration, the methanol extract of Nepenthes ampularia displayed largest inhibition zone (DIZ=18.67 ± 0.58), followed by ethyl acetate extract of N. ampularia (17.67 ± 1.15) and ethyl acetate extract of Musa gracilis (17.00 ± 1.00). The phytochemical investigation of these extracts showed the existence of tannins, flavonoids, alkaloids, terpenoids, saponins, and steroids. The pronounced anti-mycobacterial properties displayed by the screened medicinal plants scientifically proved the claim by traditional people of Endau Rompin Johor. It is suggested that the extracts may be considered for further evaluation. Keyword: Antimycobacteria; Phytochemical; Disc diffusion; Endau Rompin. 1. Introduction showed a total of 18,517 people have been infected, which is an increase of 6% when Tuberculosis (TB) is a contagious ailment compared to the previous year (17,341 cases in caused by an aerobic pathogenic bacterium the year 2009) [5]. Furthermore, the called Mycobacterium tuberculosis. This prevalence of TB was reported as 101 cases ancient disease is among the world’s most per 100 000 population in 2011 [6]. Modern deadly epidemics and can happen to anyone, therapy including isoniazid, rifampicin, irrespective of sex, age, and nationality [1], ethambutol, pyrazinamide, and streptomycin [2]. In 2015, World Health Organization are used in the treated of TB. However, these (WHO) reported that there were 10.4 million agents have shortcomings of causing side occurrences of TB around the world. About effects, and the TB-causing bacterium can 1.4 million deaths due to TB among HIV- quickly gain resistance to these drugs [7]. The negative persons were documented during increase in multi-drug resistant TB and 2015. TB accounted for one out of ten causes extensively drug-resistant TB strains of mortality globally which more when prevalence in the world is worrisome, and for compared to deadly HIV/AIDS in 2015 [3]. over 30 years there was no TB medicine More than half of the global TB cases occur in introduced into the market [8]. Thus, it is Asia region (58%), followed by Africa crucial to search for a novel antimycobacterial continent (27%). The smaller percentages agent. Due to their chemical variety and happen in the East Mediterranean (8%), important role in the anti-infective agent’s European region (4%), and America (3%) [4]. development, medicinal plants proffer great Like in any other developing country, TB is hope to overcome these need. For long, plant- still a trait to public health in Malaysia. The based medicines have been used globally in trend of TB in the year 2010 in Malaysia the treatment of different ailments. About 75% *Corresponding author: [email protected] 2018 UTHM Publisher. All right reserved. e-ISSN: 2600-7924/penerbit.uthm.edu.my/ojs/index.php/jst Journal of Science and Technology, Vol. 10 No. 2 (2018) p. 30-37 of the world’s populace depend mainly on plant medicines for their primary health care [1]. Jakun is a tribe from a subgroup of Proto- Malay that inhabit Kampung Peta, with the population of around 220 people consisting 67 family units, representing about 2% of the entire Orang Asli in Johor [9]. Jakun tribe living in Kampung Peta depends on their medicinal plants for primary healthcare to treat different diseases traditionally. There was claim by Jakun community in Kampung Peta that 23 species of medicinal plants are used in the treatment of TB and its symptoms [10]. Hence, there need to verify the claim scientifically. Thus, the present study was designed to investigate the in vitro anti- mycobacterial properties and to screen the phytochemicals present in the extracts Fig. 1 The location of Kampung Peta in Johor qualitatively. National Park Endau-Rompin 2. Materials and Methods 2.2 Samples collection and preparation The plant specimens (Table 1) were 2.1 Study area collected from Taman Negara Johor Endau The Endau Romping rainforest, situated Rompin, Johor, Endau Rompin and were border to the north-east of Endau, Johor Darul identified using the morphometric method in Takzim and south to Romping, Pahan. The which, the species are recognized based on the forest (2o25’12.94”N, 103o15’40.94”E) (Fig. characteristic of their leaf, flower shape and/or 1) is one of those few virgin lowland branching structure. The plant materials were rainforests remain in southern Peninsular cleaned, chopped into smaller pieces and then Malaysia. The state government of Johor in dried in a hot oven at 40oC-60oC for 24-72 h 1993 gazetted 870 km2 of the forest of Taman [12]–[14]. The dried samples were then Negara Johor Endau Rompin (TNJER) as a ground using a stainless steel blender into a national park. A village close to the park powder which was later sieved. The sieved called Kampung Peta becomes the major samples were weight and transferred to a entrance to the National Park. Inside the plastic container and stored at room forest lie different species of plant that offer temperature. significant sources of shelters, food, medicines, etc. to the neighboring civilization [10], [11]. Table 1 List of the medicinal plants used in the study Scientific name Local name Family Part collected Collected from Campnosperma Habong Anacardiaceae Shoot Bununt 49m auriculatum 02o31.857’N (Blume) Hook.f. 103o22.624’E Musa gracilis Pisang sum Musaceae Pseudostem Sungai Samawak Holttum 42m 02o31.7491’N 103o23.807’E Macaranga Tudung Euphorbiaceae Stem Pantai Burung 30m gigantea 02o31.864’N (Rchb.f 102o24.604’E &Zoll.)M.A. Nepenthes Sentoyot Nepenthaceae Root Lubuk Bong 43m ampularia 02o31.542’N Jack 103o21.706’E 31 Journal of Science and Technology, Vol. 10 No. 2 (2018) p. 30-37 Scaphium Kembang Sterculiaceae Stem bark Ethnobatanical macropodum semangkok garden 62m (Miq.) Beum´ 02o31.7491’N ee ex.Heyne 103o24.845’E 2.3 Extraction the stock culture of Mycobacterium smegmatis 2.3.1 Decoction and then preserved on Middlebrook 7H10 agar The aqueous extract was prepared by medium. The pure culture was then stored at immersing the plant materials in a specified 4℃ until further use. From the pure cultures, volume of distilled water in 1:4 ratios. The the inoculum was prepared by subculturing extraction mixture was gently heated to the onto Middlebrook 7H9 broth medium. The temperature of 60oC in a water bath until the bacterial suspension density was pre-adjusted volume of the water was brought down to one- to 0.5 McFarland standards. fourth its original volume [15]. Then, the mixture was cooled and strained (filtered) 2.6 Determination of Anti-mycobacterial through the Whatman no. 1 filter paper and the and Antibacterial Activity filtrate was frozen at -80oC in a freezer and Disc diffusion assay previously described then freeze-dried at -44oC using a freeze drier. by Rafael et al. (2011) with few modifications The weights of the dried crude extracts were was used to determine the antimycobacterial determined. activity of the extract. In brief, a filter paper of 5 mm was prepared and sterilized in an 2.3.2 Successive maceration autoclave for 15 minutes at 121°C. The filter The successive maceration method was papers were impregnated with 20 µL of extract used for solvent extraction. The powdered at of 12.5, 25, 50 and 100 mg/ml plant materials were sequentially macerated concentrations, and 20 µL of rifampicin (12.5, with the specified volume of n-hexane, ethyl 25, 50 and 100 µg/mL). The prepared disks acetate, and methanol in order of increasing were aseptically and carefully transferred onto polarity of the solvents in 1:5 ratios in an the Middlebrook 7H10 agar plates inoculated enclosed flask with occasional shaking. The with the cell suspension. The inoculated petri mixture was kept at room temperature for 24 dishes were sealed with parafilm and then h. This extraction procedure was repeated incubated at 37°C for 72 hours, after which the three times until complete extraction. The diameters of inhibition zone were measured mixture was then strained through a No. 1 and recorded. This test was performed in Whatman filter paper. The filtrate was later triplicate [19]. evaporated to a minimum volume a rotary evaporator set at 40oC in a water bath. The 3. Results and Discussions weights of the dried crude extracts were determined. The inhibitory effect of hexane, ethyl acetate, methanol, and water extracts of C. 2.4 Phytochemicals investigation auriculatum, M. gigantea, M.