DOCSLIB.ORG

Microbial Methanogenesis in the Sulfate-Reducing Zone of Surface Sediments Traversing the Peruvian Margin

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Microbial Methanogenesis in the Sulfate-Reducing Zone of Surface Sediments Traversing the Peruvian Margin Biogeosciences, 13, 283–299, 2016 www.biogeosciences.net/13/283/2016/ doi:10.5194/bg-13-283-2016 © Author(s) 2016. CC Attribution 3.0 License. Microbial methanogenesis in the sulfate-reducing zone of surface sediments traversing the Peruvian margin J. Maltby1, S. Sommer1, A. W. Dale1, and T. Treude1,2 1GEOMAR Helmholtz Centre for Ocean Research Kiel, Department of Marine Biogeochemistry, Wischhofstr. 1–3, 24148 Kiel, Germany 2present address: Department of Earth, Planetary and Space Sciences & Atmospheric and Oceanic Sciences, University of California, Los Angeles (UCLA), CA, USA Correspondence to: J. Maltby ([email protected]) and T. Treude ([email protected]) Received: 7 August 2015 – Published in Biogeosciences Discuss.: 9 September 2015 Revised: 10 December 2015 – Accepted: 21 December 2015 – Published: 15 January 2016 Abstract. We studied the concurrence of methanogenesis tion of hydrogenotrophic activity in the sulfate-depleted zone and sulfate reduction in surface sediments (0–25 cm below at the shallow shelf station (70 m). sea floor) at six stations (70, 145, 253, 407, 990 and 1024 m) Surface methanogenesis appeared to be correlated to the along the Peruvian margin (12◦ S). This oceanographic re- availability of labile organic matter (C = N ratio) and organic gion is characterized by high carbon export to the seafloor carbon degradation (DIC production), both of which sup- creating an extensive oxygen minimum zone (OMZ) on the port the supply of methanogenic substrates. A negative cor- shelf, both factors that could favor surface methanogenesis. relation between methanogenesis rates and dissolved oxy- Sediments sampled along the depth transect traversed areas gen in the bottom-near water was not obvious; however, of anoxic and oxic conditions in the bottom-near water. Net anoxic conditions within the OMZ might be advantageous methane production (batch incubations) and sulfate reduc- for methanogenic organisms at the sediment-water interface. tion (35S-sulfate radiotracer incubation) were determined in Our results revealed a high relevance of surface methano- the upper 0–25 cm b.s.f. of multiple cores from all stations, genesis on the shelf, where the ratio between surface to deep while deep hydrogenotrophic methanogenesis (> 30 cm b.s.f., (below sulfate penetration) methanogenic activity ranged be- 14C-bicarbonate radiotracer incubation) was determined in tween 0.13 and 105. In addition, methane concentration pro- two gravity cores at selected sites (78 and 407 m). Further- files indicated a partial release of surface methane into the more, stimulation (methanol addition) and inhibition (molyb- water column as well as consumption of methane by anaer- date addition) experiments were carried out to investigate the obic methane oxidation (AOM) in the surface sediment. The relationship between sulfate reduction and methanogenesis. present study suggests that surface methanogenesis might Highest rates of methanogenesis and sulfate reduction play a greater role in benthic methane budgeting than previ- in the surface sediments, integrated over 0–25 cm b.s.f., ously thought, especially for fueling AOM above the sulfate– were observed on the shelf (70–253 m, 0.06–0.1 and methane transition zone. 0.5-4.7 mmol m−2 d−1, respectively), while lowest rates were discovered at the deepest site (1024 m, 0.03 and 0.2 mmol m−2 d−1, respectively). The addition of methanol resulted in significantly higher surface methanogenesis ac- 1 Introduction tivity, suggesting that the process was mostly based on non- competitive substrates – i.e., substrates not used by sulfate Microbial methanogenesis represents the terminal step of or- reducers. In the deeper sediment horizons, where competi- ganic matter degradation in marine sediments (Jørgensen, tion was probably relieved due to the decrease of sulfate, the 2006). The process is entirely restricted to a small group usage of competitive substrates was confirmed by the detec- of prokaryotes within the domain of the Archaea (Thauer, 1998). Methanogens produce methane from a narrow spec- Published by Copernicus Publications on behalf of the European Geosciences Union. 284 J. Maltby et al.: Microbial methanogenesis in the sulfate-reducing zone of surface sediments trum of substrates, primarily carbon dioxide (CO2) and hy- availability of non-competitive substrates (Zinder, 1993; Van drogen (H2) (hydrogenotrophic pathway), as well as acetate Der Maarel and Hansen, 1997). Similarly, methanogenesis (acetoclastic pathway) (Zinder, 1993). In addition, methanol activity was observed within the sulfate-reducing zone of or methylated compounds such as methylamine can be uti- organic-rich sediments from the seasonally hypoxic Limfjor- lized (methylotrophic pathway) (Oremland and Polcin, 1982; den sound, northern Denmark (Jørgensen and Parkes, 2010; Buckley et al., 2008; Zinder, 1993; King et al., 1983). Sub- Jørgensen, 1977). strates for methanogenesis are produced during depolymer- The environmental relevance of surface methanogenesis is ization and fermentation of organic macromolecules (e.g., hitherto unknown. Its closeness to the sediment–water inter- sugars, vitamins, amino acids) to smaller monomeric prod- face makes it a potential source for methane emissions into ucts (Jørgensen, 2006; Schink and Zeikus, 1982; Neill et al., the water column, unless the methane is microbially con- 1978; Donnelly and Dagley, 1980). sumed before escaping the sediment (Knittel and Boetius, Acetoclastic and hydrogenotrophic methanogenesis are 2009). Methane escapes the sediment either by diffusion predominantly found in deeper sediment zones below sul- or, when methane saturation is exceeded, in the form of fate penetration, owing to the more effective utilization of gas bubbles (Whiticar, 1978; Wever and Fiedler, 1995; Judd H2 and acetate by sulfate reducers due to their higher sub- et al., 1997; Dimitrov, 2002). The fraction of methane re- strate affinity (Oremland and Polcin, 1982; Jørgensen, 2006). leased to the water column that reaches the atmosphere Methanogens can avoid competition with sulfate reducers mainly depends on water depth, as methane is also consumed by the utilization of non-competitive substrates, such as within the water column through aerobic microbial oxida- methanol or methylamines (Oremland and Polcin, 1982; tion (Reeburgh, 2007; Valentine et al., 2001). Thus, shallow King et al., 1983). Facilitated by the usage of such non- coastal areas have higher methane emission potentials than competitive substrates, sulfate reduction and methanogene- the open ocean (Bange et al., 1994) and a greater poten- sis were found to co-occur in sulfate-containing salt marsh tial to contribute to methane-dependent atmospheric warm- sediments (Oremland et al., 1982; Buckley et al., 2008; Se- ing (IPCC, 2014). nior et al., 1982). Concurrent activity of sulfate reduction In the present study we focused on the upwelling region and methanogenesis in the marine environment has mostly off the Peruvian coast, which is another example of an en- been postulated for organic-rich sediments (Mitterer, 2010; vironment where both factors that potentially favor surface Jørgensen and Parkes, 2010; Treude et al., 2009, 2005a; methanogenesis convene – i.e., a high export of organic car- Hines and Buck, 1982; Crill and Martens, 1986); however, bon and low dissolved oxygen concentrations in the bottom details on the magnitude and environmental controls of sur- water. This upwelling region represents one of the most pro- face methanogenesis are still poorly understood (Holmer and ductive systems in the world oceans, creating one of the most Kristensen, 1994; Ferdelman et al., 1997). intense oxygen minimum zones (OMZs, Kamykowski and In a study from Eckernförde Bay, southwestern Baltic Sea, Zentara, 1990; Pennington et al., 2006). Oxygen concentra- considerable in vitro methanogenic activity was observed in tions in waters impinging on the seafloor are below 20 µM or samples taken from 5 to 40 cm sediment depth (Treude et al., even reach anoxia. Research on surface sediment methano- 2005a). Although in vitro activity was measured in sulfate- genesis in upwelling regions is scarce and its potential role free setups, methanogenic activity coincided with zones of in the carbon cycling of the Peruvian OMZ is completely un- in situ sulfate reduction. The authors concluded a coexis- known. In a study from the central Chilean upwelling area tence of the two types of organisms, which could be en- (87 m water depth, 0.5–6 cm sediment depth), low methane abled through either the usage of non-competitive substrates, production rates were detected despite high sulfate reduction dormancy of methanogens until phases of sulfate depletion, activity, when the non-competitive substrate trimethylamine and/or temporal or spatial heterogeneity in the sediments. was offered (Ferdelman et al., 1997). The authors concluded Eckernförde Bay sediments feature a high input of organic that the prevailing methanogens were competing with sul- matter due to a shallow water depth (∼ 30 m) and pronounced fate reducers for H2 and with acetogens for methylamines, phytoplankton blooms in spring, summer and fall (Smetacek, explaining the overall low methanogenesis activity observed 1985). Furthermore, seasonal hypoxia (O2 < 90 µM) or even (Ferdelman et al., 1997). anoxia (O2 D0 µM) occur in the deep layers of the water col- Even though the Chilean and Peruvian OMZs are con- umn caused by stratification and degradation of organic mat- nected, commonly known as OMZ in the eastern
Load more

Recommended publications
  • Carbon Nanotubes Accelerate Acetoclastic Methanogenesis
    Carbon nanotubes accelerate acetoclastic methanogenesis: From pure cultures to anaerobic soils Leilei Xiao, Shiling Zheng, Eric Lichtfouse, Min Luo, Yang Tan, Fanghua Liu To cite this version: Leilei Xiao, Shiling Zheng, Eric Lichtfouse, Min Luo, Yang Tan, et al.. Carbon nanotubes accelerate acetoclastic methanogenesis: From pure cultures to anaerobic soils. Soil Biology and Biochemistry, Elsevier, 2020, 150, 10.1016/j.soilbio.2020.107938. hal-02930808 HAL Id: hal-02930808 https://hal.archives-ouvertes.fr/hal-02930808 Submitted on 4 Sep 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Carbon nanotubes accelerate acetoclastic methanogenesis: From pure cultures to anaerobic soils Leilei Xiao a,e,f, Shiling Zheng a,f, Eric Lichtfouse c, Min Luo d, Yang Tan f, Fanghua Liu a,b,e,f,* a Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, PR China b Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science
    [Show full text]
  • METHANOGENESIS Studies of the Process and the Micro-Organisms Involved in Methane Formation
    METHANOGENESIS Studies of the process and the micro-organisms involved in methane formation Theo Hutten PROMOTOR: PROF.DR.IR. G.D. VOGELS CO-REFERENT: DR. С VAN DER DRIFT METHANOGENESIS STUDIES OF THE PROCESS AND THE MICRO-ORGANISMS INVOLVED IN METHANE FORMATION PROEFSCHRIFT TER VERKRIJGING VAN DE GRAAD VAN DOCTOR IN DE WISKUNDE EN NATUURWETENSCHAPPEN AAN DE KATHOLIEKE UNIVERSITEIT TE NIJMEGEN, OP GEZAG VAN DE RECTOR MAGNIFICUS PROF. DR. P.G.A.B. WIJDEVELD VOLGENS BESLUIT VAN HET COLLEGE VAN DEKANEN IN HET OPENBAAR TE VERDEDIGEN OP VRIJDAG 5 FEBRUARI 1982 DES NAMIDDAGS TE 2 UUR PRECIES door THEODORUS JACOBUS HENRI MARI HUTTEN geboren te Ootmarsum 1981 Druk: Snel druk Boulevard Enschede Gelukkig mag de eerste pagina in een proefschrift besteed worden aan al degenen die gedurende mijn studietijd en gedurende mijn promotie­ onderzoek veel voor mij.hebben betekend. Dank aan alle studenten die veel van het hierna beschreven onder­ zoek hebben verricht en die voor mij het dagelijkse noodzakelijke menselijke contact hebben betekend: Jan Keltjens, Peter van de Munckhof, Wim Robbers, Mieke Heuker of Hoek, Ed IJzerman, Gerard van Keulen, John Musters, Julie Kil, Retry van Kempen, Henk Kosse, Pieter Maas, Ben Peeters, Riek Bongaerts, Leon Gorris, Willem Pauli, Johan Heemskerk, Betty Bergman, Ton Breed en Rien de Goey. Mijn collega's - Mieke, Wim, John, Federico, Annemarie, Hans, Jan, Dick, Evert-Jan, Ger, Ton, Henk, Peter, Wolfgang, Claudius, Chris en Fried - wil ik bedanken voor hun niet aflatende hulp en vele genoeg­ lijke uren die we aan de koffie en met zeilen en sporten hebben door­ gebracht. De hulp en kennis die de Afdeling Preventieve en Sociale Tandheel­ kunde (Hans en Henk) en de Afdeling Antropogenetica (Frank) hebben geboden bij de isotachophorese, is zeer waardevol geweest.
    [Show full text]
  • Methanogenesis in Oxygenated Soils Is a Substantial Fraction of Wetland Methane Emissions
    Lawrence Berkeley National Laboratory Recent Work Title Methanogenesis in oxygenated soils is a substantial fraction of wetland methane emissions. Permalink https://escholarship.org/uc/item/6rm9360v Journal Nature communications, 8(1) ISSN 2041-1723 Authors Angle, Jordan C Morin, Timothy H Solden, Lindsey M et al. Publication Date 2017-11-16 DOI 10.1038/s41467-017-01753-4 Peer reviewed eScholarship.org Powered by the California Digital Library University of California ARTICLE DOI: 10.1038/s41467-017-01753-4 OPEN Methanogenesis in oxygenated soils is a substantial fraction of wetland methane emissions Jordan C. Angle1, Timothy H. Morin2,3, Lindsey M. Solden1, Adrienne B. Narrowe4, Garrett J. Smith 1, Mikayla A. Borton1,3, Camilo Rey-Sanchez2,3, Rebecca A. Daly1, Golnazalsdat Mirfenderesgi2, David W. Hoyt 5, William J. Riley6, Christopher S. Miller 4, Gil Bohrer 2,3 & Kelly C. Wrighton1,3 The current paradigm, widely incorporated in soil biogeochemical models, is that microbial 1234567890 methanogenesis can only occur in anoxic habitats. In contrast, here we show clear geo- chemical and biological evidence for methane production in well-oxygenated soils of a freshwater wetland. A comparison of oxic to anoxic soils reveal up to ten times greater methane production and nine times more methanogenesis activity in oxygenated soils. Metagenomic and metatranscriptomic sequencing recover the first near-complete genomes for a novel methanogen species, and show acetoclastic production from this organism was the dominant methanogenesis pathway in oxygenated soils. This organism, Candidatus Methanothrix paradoxum, is prevalent across methane emitting ecosystems, suggesting a global significance. Moreover, in this wetland, we estimate that up to 80% of methane fluxes could be attributed to methanogenesis in oxygenated soils.
    [Show full text]
  • Microbial Methanogenesis in the Sulfate-Reducing Zone of Sediments in the Eckernförde Bay, SW Baltic Sea
    Biogeosciences, 15, 137–157, 2018 https://doi.org/10.5194/bg-15-137-2018 © Author(s) 2018. This work is distributed under the Creative Commons Attribution 3.0 License. Microbial methanogenesis in the sulfate-reducing zone of sediments in the Eckernförde Bay, SW Baltic Sea Johanna Maltby1,a, Lea Steinle2,1, Carolin R. Löscher3,1, Hermann W. Bange1, Martin A. Fischer4, Mark Schmidt1, and Tina Treude5,6 1GEOMAR Helmholtz Centre for Ocean Research Kiel, Department of Marine Biogeochemistry, 24148 Kiel, Germany 2Department of Environmental Sciences, University of Basel, 4056 Basel, Switzerland 3Nordic Center for Earth Evolution, University of Southern Denmark, 5230 Odense, Denmark 4Institute of Microbiology, Christian-Albrecht-University Kiel, 24118 Kiel, Germany 5Department of Earth, Planetary, and Space Sciences, University of California Los Angeles (UCLA), Los Angeles, California 90095-1567, USA 6Department of Atmospheric and Oceanic Sciences, University of California Los Angeles (UCLA), Los Angeles, California 90095-1567, USA apresent address: Natural Sciences Department, Saint Joseph’s College, Standish, Maine 04084, USA Correspondence: Johanna Maltby ([email protected]) and Tina Treude ([email protected]) Received: 4 February 2017 – Discussion started: 7 March 2017 Revised: 30 October 2017 – Accepted: 8 November 2017 – Published: 10 January 2018 Abstract. Benthic microbial methanogenesis is a known way of methanogenesis in the presence or absence of sul- source of methane in marine systems. In most sediments, fate reducers, which after the addition of a noncompetitive the majority of methanogenesis is located below the sulfate- substrate was studied in four experimental setups: (1) un- reducing zone, as sulfate reducers outcompete methanogens altered sediment batch incubations (net methanogenesis), for the major substrates hydrogen and acetate.
    [Show full text]
  • Anaerobic Digestion Basics
    CIS 1215 Anaerobic Digestion Basics Lide Chen and Howard Neibling pathogens and weed seeds associated with manure, Introduction improve manure nutrient availability to plants, and Dairy, potatoes, and sugar beets—all important sometimes generate new revenues. agricultural commodities in Idaho—also generate a huge amount of wastes. Two big waste-related What Is Anaerobic Digestion? challenges facing the dairy and food processing industries are emissions of odors and gases and Anaerobic digestion is a series of biological processes manure management. Gas emissions include that use a diverse population of bacteria to break down organic materials into biogas, primarily methane, and a greenhouse gases; ammonia (NH 3), a colorless gas with combination of solid and liquid effluents, the digestate a pungent smell; and hydrogen sulfide (H 2S), a colorless gas with the foul odor of rotten eggs. (figure 1). It occurs in the absence of free oxygen. Rising energy prices, more restrictive regulatory Organic materials are composed of organic compounds requirements, and increasing concern over greenhouse resulting from the remains or decomposition of previously gas emissions are causing many people in Idaho’s dairy living organisms such as plants and animals and their and food-processing industries to consider anaerobic waste products. Sources of organic material for anaerobic digestion (AD) of wastes from their operations. digestion include dairy manure, food processing waste, Anaerobic digestion technology is being viewed as a plant residues, and other organic wastes such as municipal way to address environmental concerns, generate wastewater, food waste, and fats, oils, and grease. renewable energy, cut bedding-material costs, reduce Other organic wastes Heat Electricity H2S removal Animal waste s ga io b ne ha et Electricity Biogas M generator Pu re m et ha Cow bedding Digester reactor ne CO and 2 Natural gas pipeline impurities High-quality, low-odor fertilizer Figure 1.
    [Show full text]
  • Methanogenic Microorganisms in Industrial Wastewater Anaerobic Treatment
    processes Review Methanogenic Microorganisms in Industrial Wastewater Anaerobic Treatment Monika Vítˇezová 1 , Anna Kohoutová 1, Tomáš Vítˇez 1,2,* , Nikola Hanišáková 1 and Ivan Kushkevych 1,* 1 Department of Experimental Biology, Faculty of Science, Masaryk University, 62500 Brno, Czech Republic; [email protected] (M.V.); [email protected] (A.K.); [email protected] (N.H.) 2 Department of Agricultural, Food and Environmental Engineering, Faculty of AgriSciences, Mendel University, 61300 Brno, Czech Republic * Correspondence: [email protected] (T.V.); [email protected] (I.K.); Tel.: +420-549-49-7177 (T.V.); +420-549-49-5315 (I.K.) Received: 31 October 2020; Accepted: 24 November 2020; Published: 26 November 2020 Abstract: Over the past decades, anaerobic biotechnology is commonly used for treating high-strength wastewaters from different industries. This biotechnology depends on interactions and co-operation between microorganisms in the anaerobic environment where many pollutants’ transformation to energy-rich biogas occurs. Properties of wastewater vary across industries and significantly affect microbiome composition in the anaerobic reactor. Methanogenic archaea play a crucial role during anaerobic wastewater treatment. The most abundant acetoclastic methanogens in the anaerobic reactors for industrial wastewater treatment are Methanosarcina sp. and Methanotrix sp. Hydrogenotrophic representatives of methanogens presented in the anaerobic reactors are characterized by a wide species diversity. Methanoculleus
    [Show full text]
  • (NPP) A) Global Patterns B) Fate of NPP
    OCN 401 Biogeochemical Systems (10.25.16) (Schlesinger: Chapter 9) Part 2. Oceanic Carbon and Nutrient Cycling Lecture Outline 1. Net Primary Production (NPP) a) Global Patterns b) Fate of NPP 2. Sediment Diagenesis a) Diagenesis of Organic Matter (OM) b) Biogenic Carbonates Net Primary Production: Global Patterns • Oceanic NPP is ≈ 50% of total NPP on Earth - mostly as phytoplankton (microscopic plants) in surface mixed layer - seaweed accounts for only ≈ 0.1%. • NPP ranges from 130 - 420 gC/m2/yr, lowest in open ocean, highest in coastal zones • Terrestrial forests range from 400-800 gC/m2/yr, while deserts average 80 gC/ m2/yr. 1 Net Primary Production: Global Patterns (cont’d.) • O2 distribution is an indirect measure of photosynthesis: CO2 + H2O = CH2O + O2 14 • NPP is usually measured using O2-bottle or C-uptake techniques. 14 • O2 bottle measurements tend to exceed C-uptake rates in the same waters. Net Primary Production: Global Patterns (cont’d.) • Controversy over magnitude of global NPP arises from discrepancies in methods for measuring NPP: estimates range from 27 to 51 x 1015 gC/yr. 14 • O2 bottle measurements tend to exceed C-uptake rates because: - large biomass of picoplankton, only recently observed, which pass through the filters used in the 14C technique. - picoplankton may account for up to 50% of oceanic production. - DOC produced by phytoplankton, a component of NPP, passes through filters. - Problems with contamination of 14C-incubated samples with toxic trace elements depress NPP. 2 Net Primary Production: Global Patterns (cont’d.) Despite disagreement on absolute magnitude of global NPP, there is consensus on the global distribution of NPP.
    [Show full text]
  • Hydrogenotrophic Methanogenesis in Archaeal Phylum Verstraetearchaeota Reveals the Shared Ancestry of All Methanogens
    Hydrogenotrophic methanogenesis in archaeal phylum Verstraetearchaeota reveals the shared ancestry of all methanogens Bojk A. Berghuisa, Feiqiao Brian Yua,b, Frederik Schulzc, Paul C. Blaineyd,e, Tanja Woykec, and Stephen R. Quakea,b,f,1 aDepartment of Bioengineering, Stanford University, Stanford, CA 94305; bChan Zuckerberg Biohub, San Francisco, CA 94158; cDepartment of Energy Joint Genome Institute, Walnut Creek, CA 94598; dDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139; eBroad Institute of Harvard and MIT, Cambridge, MA 02142; and fDepartment of Applied Physics, Stanford University, Stanford, CA 94305 Contributed by Stephen R. Quake, December 29, 2018 (sent for review September 27, 2018; reviewed by Jared R. Leadbetter and Marc Strous) Methanogenic archaea are major contributors to the global carbon electron donor (1, 11). To conserve energy, hydrogenotrophs couple cycle and were long thought to belong exclusively to the euryarchaeal the WLP to methanogenesis. This coupling is established by N5- phylum. Discovery of the methanogenesis gene cluster methyl- methyl-tetrahydromethanopterin:coenzyme M methyltransferase coenzyme M reductase (Mcr) in the Bathyarchaeota, and thereafter (Mtr; also known as tetrahydromethanopterin S-methyltransferase), the Verstraetearchaeota, led to a paradigm shift, pushing back the which transfers the methyl group from the WLP to coenzyme M. Mtr + evolutionary origin of methanogenesis to predate that of the uses the free energy of methyl transfer to establish a Na -motive Euryarchaeota. The methylotrophic methanogenesis found in force across the membrane (14). Methyl coenzyme M reductase then the non-Euryarchaota distinguished itself from the predomi- reduces methyl-coenzyme M to methane, using coenzyme B as an nantly hydrogenotrophic methanogens found in euryarchaeal orders as the former do not couple methanogenesis to carbon fixation through electron donor.
    [Show full text]
  • Rumen Methanogenesis, Rumen Fermentation, and Microbial Community Response to Nitroethane, 2-Nitroethanol, and 2-Nitro-1-Propanol: an in Vitro Study
    animals Article Rumen Methanogenesis, Rumen Fermentation, and Microbial Community Response to Nitroethane, 2-Nitroethanol, and 2-Nitro-1-Propanol: An In Vitro Study Zhenwei Zhang, Yanlu Wang, Xuemeng Si, Zhijun Cao, Shengli Li and Hongjian Yang * State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; [email protected] (Z.Z.); [email protected] (Y.W.); [email protected] (X.S.); [email protected] (Z.C.); [email protected] (S.L.) * Correspondence: [email protected] Received: 7 February 2020; Accepted: 5 March 2020; Published: 13 March 2020 Simple Summary: The present study comparatively investigates the inhibitory difference of nitroethane (NE), 2-nitroethanol (NEOH), and 2-nitro-1-propanol (NPOH) on in vitro rumen fermentation, microbial populations, and coenzyme activities associated with methanogenesis. The results showed that both NE and NEOH were more effective in reducing ruminal methane (CH4) production than NPOH. This work provides evidence that NE, NEOH, and NPOH were able to inhibit methanogen population and dramatically decrease methyl-coenzyme M reductase gene expression and the content of coenzymes F420 and F430 with different magnitudes in order to reduce ruminal CH4 production. Abstract: Nitroethane (NE), 2-nitroethanol (NEOH), and 2-nitro-1-propanol (NPOH) were comparatively examined to determine their inhibitory actions on rumen fermentation and methanogenesis in vitro. Fermentation characteristics, CH4 and total gas production, and coenzyme contents were determined at 6, 12, 24, 48, and 72 h incubation time, and the populations of ruminal microbiota were analyzed by real-time PCR at 72 h incubation time. The addition of NE, NEOH, and NPOH slowed down in vitro rumen fermentation and reduced the proportion of molar CH4 by 96.7%, 96.7%, and 41.7%, respectively (p < 0.01).
    [Show full text]
  • Abstract Methanogenic Archaea Are Major Contributors to the Global Carbon Cycle and Were Long Thought to Belong Exclusively to the Euryarchaeotal Phylum
    bioRxiv preprint doi: https://doi.org/10.1101/391417; this version posted August 14, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Hydrogenotrophic methanogenesis in archaeal phylum Verstraetearchaeota reveals the shared ancestry of all methanogens 1 1,2 3 4 Bojk A. Berghuis ,​ Feiqiao Brian Yu ,​ Frederik Schulz ,​ Paul C. Blainey ,​ ​ 3 ​ 1,2,5​ ​ Tanja Woyke ,​ Stephen R. Quake ​ ​ 1 Department​ of Bioengineering, Stanford University, Stanford, United States 2 Chan​ Zuckerberg Biohub, San Francisco, United States 3 Department​ of Energy Joint Genome Institute, Walnut Creek, United States 4 MIT​ Department of Biological Engineering and Broad Institute of Harvard and MIT 5 Department​ of Applied Physics, Stanford University, Stanford, United States Abstract Methanogenic archaea are major contributors to the global carbon cycle and were long thought to belong exclusively to the euryarchaeotal phylum. Discovery of the methanogenesis gene cluster methyl-coenzyme M reductase (Mcr) in the Bathyarchaeota and thereafter the Verstraetearchaeota led to a paradigm shift, pushing back the evolutionary origin of methanogenesis to pre-date that of the Euryarchaeota. The methylotrophic methanogenesis found in the non-Euryarchaota distinguished itself from the predominantly hydrogenotrophic methanogens found in euryarchaeal orders as the former do not couple methanogenesis to carbon fixation through the reductive acetyl-coenzyme A (Wood-Ljungdahl) pathway, which was interpreted as evidence for independent evolution of the two methanogenesis pathways. Here, we report the discovery of a complete and divergent hydrogenotrophic methanogenesis pathway in a novel, thermophilic order of the Verstraetearchaeota which we have named Candidatus Methanohydrogenales, as well as the ​ ​ presence of the Wood-Ljungdahl pathway in the crenarchaeal order Desulfurococcales.
    [Show full text]
  • Methanogenesis and Methane Oxidation in Wetlands. Implications in the Global Carbon Cycle
    TORRES OK 09 11/11/05 5:39 PM Page 327 Hidrobiológica 15 (3): 327-349 ARTÍCULO DE REVISIÓN Diciembre 2005 Methanogenesis and methane oxidation in wetlands. Implications in the global carbon cycle Metanogénesis y metano-oxidación en humedales. Implicaciones en el ciclo del carbono global Rocio Torres-Alvarado1, Florina Ramírez-Vives2, Francisco José Fernández 2 e Irene Barriga-Sosa1 1Departamento de Hidrobiología y 2Departamento de Biotecnología. Universidad Autónoma Metropolitana-Iztapalapa (UAM-I). Av. San Rafael Atlixco # 186.Col. Vicentina. A. P. 55 535. Ciudad de México. 09430, México. Torres-Alvarado R., F. Ramírez-Vives, F. J. Fernández e I. Barriga-Sosa. 2005. Methanogenesis and Methane Oxidation in Wetlands. Implications in the Global Carbon Cycle. Hidrobiológica 15 (3): 327-349. ABSTRACT Wetlands are important ecosystems on the Earth. They are distinguished by the presence of water, saturated anoxic soils, and different kinds of vegetation adapted to this conditions. Organic matter in these environments is mineralized mainly in the sediments throughout anaerobic processes where sulfate reduction is one of the most important terminal stages of anaerobic decomposition in coastal wetlands, whereas methanogenesis is important in freshwater wetlands. In this environments, methane, a greenhouse gas, is produced as a result of the activity of a large and diverse group of methanogenic bacteria (Domain Archaea). The generated methane can be diffused to the atmosphere or can be oxidized by several microorganisms under aerobic and anaerobic conditions, such microorganisms intercept and consume this gas diminishing its emission to the atmosphere. The production and consumption of methane in wetlands involve complex physiological processes of plants and microorganisms, which are regulated by climatic and edaphic factors, mainly soil temperature and water table level.
    [Show full text]
  • S41598-021-87118-W.Pdf
    www.nature.com/scientificreports OPEN Characterization and signifcance of extracellular polymeric substances, reactive oxygen species, and extracellular electron transfer in methanogenic biocathode Basem S. Zakaria & Bipro Ranjan Dhar* The microbial electrolysis cell assisted anaerobic digestion holds great promises over conventional anaerobic digestion. This article reports an experimental investigation of extracellular polymeric substances (EPS), reactive oxygen species (ROS), and the expression of genes associated with extracellular electron transfer (EET) in methanogenic biocathodes. The MEC-AD systems were examined using two cathode materials: carbon fbers and stainless-steel mesh. A higher abundance of hydrogenotrophic Methanobacterium sp. and homoacetogenic Acetobacterium sp. appeared to play a major role in superior methanogenesis from stainless steel biocathode than carbon fbers. Moreover, the higher secretion of EPS accompanied by the lower ROS level in stainless steel biocathode indicated that higher EPS perhaps protected cells from harsh metabolic conditions (possibly unfavorable local pH) induced by faster catalysis of hydrogen evolution reaction. In contrast, EET-associated gene expression patterns were comparable in both biocathodes. Thus, these results indicated hydrogenotrophic methanogenesis is the key mechanism, while cathodic EET has a trivial role in distinguishing performances between two cathode electrodes. These results provide new insights into the efcient methanogenic biocathode development. Te concept of electro-methanogenesis
    [Show full text]