Chondrial Ca2+ Overload. Eur J Ph
誌 上 発 表 ( 原 著 論 文 ) 185 誌 上 発 表 (原著論文) Summaries of Papers Published in Other Journals (Original Papers) Namekata, I.*, Shimada, H.*, Kawanishi, T., Tanaka, H.* were in good agreement with those previously reported in and Shigenobu, K*: Reduction by SEA0400 of myo- voltage clamped cardiomyocytes. cardial ischemia-induced cytoplasmic and mito- Keywords: Na+-Ca2+ exchange, cardiomyocyte, fl uorescence chondrial Ca2+ overload. microscopy ( ) *1 Eur J Pharmacol ., 543, 108-115 2006 東邦大学薬学部 + The cardioprotective effects of SEA0400, a novel Na - *2 大妻大学家政学部 Ca2+ exchanger inhibitor, were examined in isolated guinea pig myocardial tissue and ventricular myocytes. In a coro- Tanaka, H.*1, Shimada, H.*1, Namekata, I.*1, Kawanishi, T., nary-perfused right ventricular tissue preparation, SEA0400 Iida-Tanaka, N.*2, Shigenobu, K.*1: Involvement of the had no cardiosuppressive effect during normoxia and exper- Na+/Ca2+ exchanger in ouabain-induced inotropy imental ischemia, but enhanced the recovery of contractile and arrhythmogenesis in guinea-pig myocardium force during reperfusion. SEA0400 had no effect on tissue as revealed by SEA0400. ATP content during normoxia, but attenuated its decrease J Pharmacol Sci. , 103, 241-246( 2007) during ischemia. Treatment of ventricular myocytes with Involvement of the Na+/Ca2+ exchanger in ouabain- an ischemia mimetic solution( high K+, glucose free, pH induced inotropy and arrhythmogenesis was examined with 6.0, gassed with N2) resulted in the depolarization of the a specific inhibitor, SEA0400. In right ventricular papil- mitochondrial membrane potential and an increase in cyto- lary muscle isolated from guinea-pig ventricle, 1 microM plasmic and mitochondrial Ca2+ concentration, which had a SEA0400, which specifi cally inhibits the Na+/Ca2+ exchang- similar time course.
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