Impacts of Asiatic Sand Sedge on Native Plants and Arbuscular Mycorrhizal Fungi in a Barrier Dune

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Impacts of Asiatic Sand Sedge on Native Plants and Arbuscular Mycorrhizal Fungi in a Barrier Dune University of Rhode Island DigitalCommons@URI Open Access Master's Theses 2011 IMPACTS OF ASIATIC SAND SEDGE ON NATIVE PLANTS AND ARBUSCULAR MYCORRHIZAL FUNGI IN A BARRIER DUNE William Johnson University of Rhode Island, [email protected] Follow this and additional works at: https://digitalcommons.uri.edu/theses Recommended Citation Johnson, William, "IMPACTS OF ASIATIC SAND SEDGE ON NATIVE PLANTS AND ARBUSCULAR MYCORRHIZAL FUNGI IN A BARRIER DUNE" (2011). Open Access Master's Theses. Paper 104. https://digitalcommons.uri.edu/theses/104 This Thesis is brought to you for free and open access by DigitalCommons@URI. It has been accepted for inclusion in Open Access Master's Theses by an authorized administrator of DigitalCommons@URI. For more information, please contact [email protected]. IMPACTS OF ASIATIC SAND SEDGE ON NATIVE PLANTS AND ARBUSCULAR MYCORRHIZAL FUNGI IN A BARRIER DUNE BY WILLIAM JOHNSON A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE IN BIOLOGICAL SCIENCES UNIVERSITY OF RHODE ISLAND 2011 MASTER OF SCIENCE THESIS OF WILLIAM JOHNSON APPROVED: Thesis Committee: Major Professor_____Richard E. Koske______________ _____ Keith T. Killingbeck ___________ _____Laura A. Meyerson ____________ _____Nasser H. Zawia_______________ DEAN OF THE GRADUATE SCHOOL UNIVERSITY OF RHODE ISLAND 2011 ABSTRACT The recent expansion of the nonnative invasive Asiatic sand sedge (Carex kobomugi Ohwi) at East Beach State Park, Rhode Island, is reducing populations of the most important native, dune-building species and their associated arbuscular mycorrhizal fungi (AMF). In contrast to the native American beachgrass (Ammophila breviligulata Fern.) that is dependent upon AMF to thrive in nutrient- poor sand dunes, C. kobomugi does not form beneficial associations with the fungi. Furthermore, assessments suggest that the sedge is competitively superior in obtaining the essential nutrient phosphorous without AMF-facilitation. Analysis of data from transects of the dune system revealed significant negative correlations between distributions of C. kobomugi and A. breviligulata that are being extirpated. Percent cover of A. breviligulata was significantly reduced in areas of C. kobomugi. Other native plant species were not significantly reduced as a result of C. kobomugi expansion. Spore populations of AMF showed significant positive correlations with percent cover of A. breviligulata and significant negative correlations with percent cover of C. kobomugi. Mean spore abundance of AMF in areas of C. kobomugi was less than in areas dominated by A. breviligulata. The number of species of AMF was not significantly reduced as a result of C. kobomugi likely because of highly aggregated and infrequent distribution of some species‟ spores. Assessment of mycorrhizal inoculum potential (MIP) of soils taken from the field mirrored the spore-population data: mean root colonization of plants grown in field soil of C. kobomugi (12%-24%) was between three and five times lower than that of plants grown in field soil of A. breviligulata (55%-72%). This study was unique in quantifying the effect of an invasive species on populations of mycorrhizal fungi in a dune habitat. It was novel in assessing the reduction of native plant and fungi species by C. kobomugi in Rhode Island. The replacement of AMF-forming species on dunes by a species that does not form AMF (and support spore production by these obligately biotrophic fungi) will have serious consequences when attempts are made to re-establish native species in the sites that are eventually cleared of C. kobomugi. ACKNOWLEDGMENTS I thank Dr. Richard Koske for his enthusiasm and guidance throughout this project. His personality and expertise helped to facilitate this study greatly, and as a mentor he helped to hone my skills as an active contributor to the scientific field. I thank Dr. Keith Killingbeck, Dr. Laura Meyerson and Dr. Jane Gemma for suggestions that laid the foundation that I was able to expand upon. Their ideas helped to define and better communicate my findings. I thank my lab and teaching colleagues over the years for their support as well. This study would not have been possible without material assistance provided by the University of Rhode Island. I thank Rick Enser for field notes pertaining to vegetation, Robert Paquette of the R.I. Department of Environmental Management Division of Parks and Recreation for permitting access to the dunes at East Beach. I thank Warren Hall of the Town of Middletown, and Andrew MacLachlan of the U.S. Fish and Wildlife Service for information concerning Sachuest Point and East Beach respectively. Lastly, this thesis would not have been possible without the support from my friends and family. iv PREFACE This thesis is written in the manuscript style format approved by the Graduate School of the University of Rhode Island with modifications in style as required for publication in the specific journal listed. One manuscript is as follows: MANUSCRIPT I. “Impacts of Asiatic sand sedge on native plants and arbuscular mycorrhizal fungi in a barrier dune (to be submitted in the Journal of Coastal Research). Detailed methods for certain aspects of the study are presented in the Appendices. v TABLE OF CONTENTS ABSTRACT .................................................................................................................. ii ACKNOWLEDGMENTS .......................................................................................... iv PREFACE ..................................................................................................................... v TABLE OF CONTENTS............................................................................................ vi LIST OF TABLES ...................................................................................................... ix LIST OF FIGURES ..................................................................................................... x MANUSCRIPT I. ......................................................................................................... 1 ABSTRACT .................................................................................................................. 2 ADDITIONAL INDEX WORDS ................................................................................ 3 INTRODUCTION ........................................................................................................ 4 Mycorrhizal Fungi ....................................................................................................... 4 Ammophila breviligulata and Carex kobomugi........................................................... 6 MATERIALS AND METHODS .............................................................................. 17 Study Site .................................................................................................................... 17 Vegetation Percent Cover Analysis .......................................................................... 18 Spore Abundance and Species Richness of AMF (2009) ........................................ 20 Spore Abundance and Species Richness of AMF (2010) ........................................ 21 Spore Extraction ........................................................................................................ 22 Spore Identification and Analysis ............................................................................. 24 Mycorrhizal Inoculum Potential (MIP) ................................................................... 25 Field Plant Leaf Tissue Phosphate ........................................................................... 28 Available Soil Phosphorous (2009) ........................................................................... 31 vi Statistical Analysis ..................................................................................................... 31 RESULTS ................................................................................................................... 33 Vegetation Percent Cover .......................................................................................... 33 Spore Abundance and Species Richness of AMF .................................................... 33 MIP Growth Experiment .......................................................................................... 35 Soil Phosphate ............................................................................................................ 36 Field Plant Leaf Tissue Phosphate ........................................................................... 36 DISCUSSION ............................................................................................................. 38 Effects of Invasive Plants on Belowground Biota and Processes ........................... 38 Reduction of A. breviligulata and AMF by C. kobomugi ........................................ 42 Effects of Replacement and Reduction of A. breviligulata ..................................... 46 Spread of C. kobomugi and Implications for Future Mitigation ........................... 48 ACKNOWLEDGEMENTS....................................................................................... 55 APPENDICES ............................................................................................................ 56 Appendix A: Root Staining Procedure (adapted and modified from Koske and Gemma, 1989 and Schmidt and Reeves, 1984): ................................................. 56 Appendix B:
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