Amphibia, Anura, Hylidae, Argenteohyla Siemersi Pederseni

Home , Corrientes Province, Lysapsus limellum, Santa Fe Province

n Argenteohyla siemersi pederseni

istributio Amphibia, Anura, Hylidae, D (Williams and Bosso, 1994): first record and some

raphic 1 g hematological data in Santa Fe Province, Argentina 1,2* 1 1,2 1,2 eo

G Rafael C.1,2 Lajmanovích , Paola P. Peltzer , Andrés M. Attademo , Mariana C. Cabagna , Celina M. n o

Junges and Agustìn Basso otes 1 Facultad de Bioquímica y [email protected] Biológicas (FBCB-UNL), Cátedra de Ecotoxicología. Paraje “El Pozo” s/n. CP 3000 Santa Fe, Argentina. N 2 Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Av. Rivadavia 1917. CP C1033AAJ. Buenos Aires, Argentina * Corresponding author. E-mail:

Abstract: Argenteohyla siemersi pederseni

We herein report on the first west-southern most record of in Argentina. This finding was recorded ca. 250 km far from the nearest known locality in Corrientes, and also enlarges the distribution range for including for the Santa Fe Province.

Argenteohyla siemersi pederseni is an uncommon hylid et al. A. siemersi pederseni in diversities (Arturi 2006). Rhinella foundfrog classified in as Endangered (IUCN 2008; Lavilla fernandezaeWe collectedLeptodactylus this specimen latrans, of L. chaquensis, L. in2008). The more known populations of this species are latinasus,the habitat Physalaemusshared with other albonotatus, anurans such Dendropsophus as north-west of Corrientes Province (Argentina) sanborni, D. nanus,, Hypsiboas pulchellus, Lysapsus limellum, San Roque, Sanet Cosme, al. Corrientes Capital and Parque Scinax nasicus, S. squalirostris, S. acuminatus, and Pseudis Nacional MburucuyaA. s. localitiespederseni (e.g. Williams and Bosso paradoxa. 1994; Céspedez 1995; Céspedez 2000). Some natural history of was pointed by Díminich The specimen (Body weight = 4 g, Snout vent and Zaracho (2008) and Zaracho andet al. Areta (2008) who length = 41.25 mm, Head width = 14.5 mm, Femur length described its reproductive mode and its advertisement = 17 mm, Tibio-fibula length = 20 mm, Foot length = 28.5 call, respectively. Recently,a Cajade (2010) describes mm, Inter-narial distance = 2.65 mm, Narial-eye distance other reproductive traits such as egg number per clutch, = 4.25 mm, Tympanum diameter = 2.4 mm, Inter-orbital Schinopsistadpole’s morphology, balansae and lso other general bioecological distance = 6.50 mm, Eye diameter = 3.75 mm) is deposited characteristics in a modified “quebracho” forest of in the herpetological collection of Faculty of Biochemistry A. siemersi in pederseniCorrientes Province. and Biological Sciences (PL-FBCB 3709). Herein, we present the first west-southern most record of for Santa Fe Province in Argentina. TheA. s. nightpederseni of 1 February 2012 (temperature 25.5 °C; relative humidity 75 % ), we collected one specimen of (Figure 1) near marshes locate at 10.5 km approximately apart from Provincial Road N° 1 (31°10’21.10” S, 60°15’31.73”et al. W Cayastá, Garay Department, Santa Fe Province, Figure 2). According to the Ecoregions scheme (Burkart 1999), the areaProsopis present affinisintermixedP. nigra floristicP. albacommunitiesSalix humboldtiana of the Espinal andAcacia Delta cavenand Islas del Río Paraná mainly characterize by Eichhornia, crassipes, , Pontederia cordata, andSagittaria montevidensis trees. TheCyperus ephemeral corymbosus ponds andSalvinia marshes biloba present Pistia stratiotes , , , , , and . The climate is humid to sub-humid and warm to temperate (Cáceres 1980). Theet disturbancesal. in etthe al. area are due to agriculture expansion, lumbering and mainly livestocke.g. Gleditsia over-grazing triacanthos, (Hilgbert Parkinsonia 2003; aculeata Lewis Figure 1. Argenteohyla siemersi pederseni Eucaliptus 2006; Figure 3). In addition, the invasion of exotic species ( , Individual of (PL-FBCB 3709) spp.) (Pers. Obs.) is notable. These disturbances collected in Cayastá field, Santa Fe Province, Argentina. Photo by Paola M. Peltzer critically jeopardize conservation of floristic and faunistical 790 Lajmanovich et al. | Argenteohyla siemersi pederseni in Santa Fe, Argentina

H. pulchellus and H. raniceps unpublished data Previously, frog was anesthetized in MS222 (0.1%) those obtained for et al. (Cabagna solution buffered with 0.2% NaHCO3, a blood sample was ) and within range of the few determined collected by cardiac puncture using a heparinized needle. for hylid frogs (Glomski 1997). Two peripheral blood smears were prepared on clean Although this finding significantly enlarges the known slides, fixed and stained by the May-Grunwald-Giemsa distribution of this endangered frog (IUCN 2008) for 250 method (Dacie and Lewis 1991). Mature erythrocytes are km to the west-south, and constitutes the first record for oval cells with centrally placed and similarly shaped nuclei Santa Fe Province, we are conducting studies to determine (Figure 4). The size of the Red Blood 2 Cell (RBC mean ± SD, if this species has a stable population in this area. This N = 2000) was 242.112 ± 23.41 µm and the nuclei were: is especially important because major parts of this 56.22 ± 6.17 µm . These values were above the average region are not included in the conservation action plans recorded for adult anurans in this area and similar to where modification by anthropic activities gradually and profoundly changed the quality of aquatic systems by pesticide contaminations, composition of the vegetative communities either by over-grazing the herbaceous layer, eliminating the hardiest forest species or by burning all of Acknowledgmentsthe forest for agriculture.: We thank Rafael Lajmanovich (Jr) and German PeltzerLiterature for field Cited assistance.

In Arturi,La situaciónM.F. 2006. ambiental Situación argentina ambiental 2005 en la Ecorregión Espinal; p 240- 246 A.D. Brown, U. Martínez Ortiz, M. Acerbi and J. Corcuera (ed.). . Buenos Aires: FundaciónEcorregiones Vida deSilvestre la Argentina Argentina. Burkart,PRODIA R., N.O. Bárbaro, R.O. Sánchez and D.A. Gómez. 1999. . BuenosCaracterización Aires: Administración climática de la de provincia Parques de Nacionales. Santa Fe , 1-43. Cáceres, L.M. 1980. . Santa Fe: Ed. Ministerio Agricultura y Ganadería Santa Fe. Dpto. de Figure 2. Aguas. 35 p. Argenteohyla siemersi pederseni Argenteohyla siemersi pederseni Cajade, R., E.F Schaefer, M.I. Duré, A.I Kehr and F. Marangoni.Journal 2010. Geographical distribution map of the new record of ofReproductive Natural History biology of Williams in Santa Fe Province. and Bosso, 1994 (Anura: Hylidae) in northeastern Argentina. 44 (31-32): 1953-1978. Céspedes, J.A., R.H.FACENA Aguirre and B.B. Alvarez. 1995. Composición y Distribución de la Anfibiofauna de la Provincia deArgenteohyla Corrientes siemersi(Argentina). pederseni 11: 25-49. Boletín Céspedez,de la Asociación J.A. 2000. HistoriaHerpetológica natural Española de la rana de Pedersen (Anura: Hylidae),Practical y descripciónHaematology de su larva. 11(2): 75-80. Dacie, S.J. and S.M. Lewis. 1991. Argenteohyla siemersi. Seventh pederseni edition, Edinburgh: Churchill Livingstone. 524 p.Herpetological Review Díminich, M.C. and V.H. Zaracho. 2008. . Reproduction. Natural History Notes. 39 (1): 74-75. García, M.S., P.E. Leva, M.E. Zbrun, M.A. Veles, J.A. Gandolfo and S.E. Figure 3. Argenteohyla siemersi pederseni Revista FAVE - Ciencias Agrarias Valtorta. 2002. Meteorologic and biometeorologic indexes trends in Habitat of at the Garay Santa Fe-Córdoba milking area. 1(1): Departament, Santa Fe Province, mideastern Argentina. Photo by Paola 27-36. . M. Peltzer Glomski,Histology C.A., and J. Histopathology Tamburlin, R. Hard and M. Chainani. 1997. The phylogenetic odyssey of the erythrocyte IV. The amphibians. 12(1): 147-170. Hilgert, N., J. Pensiero, G.Interciencia Marino, J.P. Lewis and C. D’Angelo. 2003. VegetationIUCN of the Red saladillo List of area Threatened (Province Species of Santa Fe) in the south of the Chaco, Argentina. 28(9): 511-520. IUCN. 2008. . Electronic Database Argenteohylaaccessible at http://iucnredlist.org/siemersi . Captured on 6 In February 2012. Lavilla, E.J., J.A. Céspedez, D. Baldo, B. Blotto and J. Langone. 2008. Threatened (Mertens, Amphibians 1937); of the p. World 239. S.N. Stuart, M. Hoffmann, J.S. Chanson, N.A Cox, R.J. Berridge, P. Ramani and B.E. Young (ed.). . Barcelona: Lynx Edicions. Lewis, J.P., E.F. Pire, I.M. Barberis and D.E.Revista Prado. de 2006. Investigaciones Los bosques de del la FacultadEspinal perieste de Ciencia ´pico Agronómicas en las proximidades UNR de la localidad de Coronda, provincia de Santa Fe (Argentina). Argenteohyla siemersi 6: 13-26. Williams, J. and A. Bosso.Cuadernos 1994. de Estado Herpetología sistemático y distribución de (Mertens, 1937) en la RepúblicaArgenteohyla Argentina siemersi(Anura: Hylidae).pederseni 8 (1): 57-62. Zaracho, V.H. and J.I. Areta.FACENA 2008. The advertisement call of (Amphibia, Anura, Hylidae), and comments on its taxonomic status. 24: 49-57. Figure 4. Argenteohyla Received: February 2012 siemersi pederseni Accepted: June 2012 Erythrocytes found in blood samples of frog Published online: August 2012 (1000 x). Editorial responsibility: Raúl Maneyro 791