G C A T T A C G G C A T genes Article Calves Infected with Virulent and Attenuated Mycoplasma bovis Strains Have Upregulated Th17 Inflammatory and Th1 Protective Responses, Respectively Jin Chao 1,2,3,4, Xiaoxiao Han 1,2, Kai Liu 1,2, Qingni Li 1,2, Qingjie Peng 5, Siyi Lu 1,2, Gang Zhao 1,2, Xifang Zhu 1,2, Guyue Hu 1,2, Yaqi Dong 1,2, Changmin Hu 2, Yingyu Chen 1,2, Jianguo Chen 2, Farhan Anwar Khan 1, Huanchun Chen 1,2,3,4 and Aizhen Guo 1,2,3,4,* 1 The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China 2 College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China 3 Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan 430070, China 4 Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China 5 Wuhan Keqian Biology Ltd., Wuhan 430223, China * Correspondence:
[email protected]; Tel.: +86-27-8728-6861 Received: 15 June 2019; Accepted: 27 August 2019; Published: 28 August 2019 Abstract: Mycoplasma bovis is a critical bovine pathogen, but its pathogenesis remains poorly understood. Here, the virulent HB0801 (P1) and attenuated HB0801-P150 (P150) strains of M. bovis were used to explore the potential pathogenesis and effect of induced immunity from calves’ differential transcriptomes post infection. Nine one-month-old male calves were infected with P1, P150, or mock-infected with medium and euthanized at 60 days post-infection.