IGFBP6 Antibody Cat

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IGFBP6 Antibody Cat. No.: 45-764 IGFBP6 Antibody 45-764 (5ug/ml) staining of paraffin HEK293 overexpressing IGFBP6 and probed with 45-764 (mock embedded Human Skeletal Muscle. Steamed transfection in first lane). antigen retrieval with citrate buffer pH 6, AP- staining. Specifications HOST SPECIES: Goat SPECIES REACTIVITY: Human HOMOLOGY: Expected Species Reactivity based on sequence homology: Cow IMMUNOGEN: The immunogen for this antibody is: C-RPQDVNRRDQQRNP TESTED APPLICATIONS: ELISA, IHC, WB Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 25kDa band observed in Human Adrenal Gland lysates (calculated MW of 25.3kDa according to NP_002169.1). In transfected HEK293 transiently expressing IGFBP6 a band of approx. APPLICATIONS: 29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5ug/ml. October 2, 2021 1 https://www.prosci-inc.com/igfbp6-antibody-45-764.html SPECIFICITY: #To_Delete#. POSITIVE CONTROL: 1) Cat. No. XBL-11050 - Human Adrenal Tissue Lysate Properties Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing. CONCENTRATION: 500 ug/mL STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing. Additional Info OFFICIAL SYMBOL: IGFBP6 ALTERNATE NAMES: IGFBP6 , insulin-like growth factor binding protein 6 , IBP6, IGF binding protein 6 ACCESSION NO.: NP_002169.1 PROTEIN GI NO.: 11321593 GENE ID: 3489 Background and References 1) Cheung C, Vesey D, Cotterill A, Douglas M, Gobe G, Nicol D, Johnson D. Altered REFERENCES: messenger RNA and protein expressions for insulin-like growth factor family members in clear cell and papillary renal cell carcinomas. Int J Urol. 2005 Jan;12(1):17-28. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 2, 2021 2 https://www.prosci-inc.com/igfbp6-antibody-45-764.html.

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Original Article Serum Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) Is Increased in Patients with Type 1 Diabetes and Its Complications
Int J Clin Exp Med 2012;5(3):229-237 www.ijcem.com /ISSN:1940-5901/IJCEM1204003 Original Article Serum insulin-like growth factor binding protein 6 (IGFBP6) is increased in patients with type 1 diabetes and its complications Shangsu Lu1,2,3,6, Sharad Purohit3,4,6, Ashok Sharma3,4,6, Wenbo Zhi3,4,6, Mingfang He2,3, Yiqian Wang5, Chao-Jun Li1, Jin-Xiong She3,4 1Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal Univer- sity, Nanjing 210046, China; 2Jiangsu Institute of Translational Medicine and School of Pharmaceutical Sciences, Nanjing University of Technology, Nanjing, Jiangsu, China; 3Center for Biotechnology and Genomic Medicine and 4Department of Pathology, Medical College of Georgia, Georgia Health Sciences University, Augusta, Georgia, United States of America; 5Zhangjiagang entry-exit inspection and Quarantine Bureau, Zhangjiagang, 215600, China; 6These authors contributed equally to this study. Received April 3, 2012; accepted May 15, 2012; Epub June 10, 2012; Published June 30, 2012 Abstract: Insulin-like growth factor binding proteins (IGFBPs) are associated with insulin resistance and accelerated micro- and macro-vascular complications of diabetes. We investigated the relationship between serum levels of IGFBP6 in type-1 diabetes (T1D) patients and diabetic complications. In this study, IGFBP6 was measured in the sera from 697 T1D patients and 681 healthy controls using a Luminex assay. Mean serum levels of IGFBP6 were higher in T1D patients than controls matched for sex and age (119.7 vs 130.6 ng/ml, p < 10-4). Subject age, sex and duration of disease have a significant impact on serum IGFBP6 levels in both T1D patients and healthy controls.
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Insulin-Like Growth Factor Axis in Pregnancies Affected by Fetal Growth Disorders Aamod R
Nawathe et al. Clinical Epigenetics (2016) 8:11 DOI 10.1186/s13148-016-0178-5 RESEARCH Open Access Insulin-like growth factor axis in pregnancies affected by fetal growth disorders Aamod R. Nawathe1,2, Mark Christian3, Sung Hye Kim2, Mark Johnson1,2, Makrina D. Savvidou1,2 and Vasso Terzidou1,2* Abstract Background: Insulin-like growth factors 1 and 2 (IGF1 and IGF2) and their binding proteins (IGFBPs) are expressed in the placenta and known to regulate fetal growth. DNA methylation is an epigenetic mechanism which involves addition of methyl group to a cytosine base in the DNA forming a methylated cytosine-phosphate-guanine (CpG) dinucleotide which is known to silence gene expression. This silences gene expression, potentially altering the expression of IGFs and their binding proteins. This study investigates the relationship between DNA methylation of components of the IGF axis in the placenta and disorders in fetal growth. Placental samples were obtained from cord insertions immediately after delivery from appropriate, small (defined as birthweight <10th percentile for the gestation [SGA]) and macrosomic (defined as birthweight > the 90th percentile for the gestation [LGA]) neonates. Placental DNA methylation, mRNA expression and protein levels of components of the IGF axis were determined by pyrosequencing, rtPCR and Western blotting. Results: In the placenta from small for gestational age (SGA) neonates (n = 16), mRNA and protein levels of IGF1 were lower and of IGFBPs (1, 2, 3, 4 and 7) were higher (p < 0.05) compared to appropriately grown neonates (n =37).In contrast, in the placenta from large for gestational age (LGA) neonates (n = 20), mRNA and protein levels of IGF1 was not different and those of IGFBPs (1, 2, 3 and 4) were lower (p < 0.05) compared to appropriately grown neonates.
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Immuno-Oncology Panel 4 (Direct-MRM MS, Pending)
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Development and Validation of a Protein-Based Risk Score for Cardiovascular Outcomes Among Patients with Stable Coronary Heart Disease
Supplementary Online Content Ganz P, Heidecker B, Hveem K, et al. Development and validation of a protein-based risk score for cardiovascular outcomes among patients with stable coronary heart disease. JAMA. doi: 10.1001/jama.2016.5951 eTable 1. List of 1130 Proteins Measured by Somalogic’s Modified Aptamer-Based Proteomic Assay eTable 2. Coefficients for Weibull Recalibration Model Applied to 9-Protein Model eFigure 1. Median Protein Levels in Derivation and Validation Cohort eTable 3. Coefficients for the Recalibration Model Applied to Refit Framingham eFigure 2. Calibration Plots for the Refit Framingham Model eTable 4. List of 200 Proteins Associated With the Risk of MI, Stroke, Heart Failure, and Death eFigure 3. Hazard Ratios of Lasso Selected Proteins for Primary End Point of MI, Stroke, Heart Failure, and Death eFigure 4. 9-Protein Prognostic Model Hazard Ratios Adjusted for Framingham Variables eFigure 5. 9-Protein Risk Scores by Event Type This supplementary material has been provided by the authors to give readers additional information about their work. Downloaded From: https://jamanetwork.com/ on 10/02/2021 Supplemental Material Table of Contents 1 Study Design and Data Processing ......................................................................................................... 3 2 Table of 1130 Proteins Measured .......................................................................................................... 4 3 Variable Selection and Statistical Modeling ........................................................................................
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Supplementary Table 2
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Figure S1. Reverse Transcription‑Quantitative PCR Analysis of ETV5 Mrna Expression Levels in Parental and ETV5 Stable Transfectants
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Insulin-Like Growth Factor Binding Protein (Igfbp6) Is a Cross-Species Tendon Marker
EuropeanAJ Turlo et Cells al. and Materials Vol. 38 2019 (pages 123-136) DOI: 10.22203/eCM.v038a10 IGFBP6 as ISSN tendon 1473-2262 marker INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN (IGFBP6) IS A CROSS-SPECIES TENDON MARKER A.J. Turlo*, A.J. Mueller-Breckenridge, D.E. Zamboulis, S.R. Tew, E.G. Canty-Laird and P.D. Clegg Musculoskeletal Biology I, Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool, UK Abstract The main challenge in tendon injury management is suboptimal tissue healing that fails to re-establish original tendon function. Tissue bioengineering is a promising approach for tendon therapy, with potential to improve its functional outcomes. However, evaluation criteria for tissue-engineered tendon are unclear due to the lack of specific markers of differentiated tendon. The study aim was to identify a panel of genes that characterised tendons in comparison to cartilage or muscles and validate those genes, both in human and key species used as models for tendon diseases. Gene expression profiling of rat tendon and cartilage in whole-tissue samples and primary tenocytes and chondrocytes was undertaken using two independent microarray platforms. Genes that demonstrated high expression correlation across two assays were validated by qRT-PCR in rat tendon relative to cartilage and muscle. Five genes demonstrating the highest tendon- related expression in the validation experiment (ASPN, ECM1, IGFBP6, TNMD, THBS4) were further evalu- ated by qRT-PCR in ovine, equine and human tissue. The group of tendon markers, identified by unbiased transcriptomic analysis of rat musculoskeletal tissues, demonstrated species-dependent profiles of expression. Insulin-like growth factor binding protein 6 (IGFBP6) was identified as the only universal tendon marker.
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Characterization and Expression Analysis of Insulin Growth Factor Binding Proteins (Igfbps) in Pacific White Shrimp Litopenaeus Vannamei
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The Role of Insulin-Like Growth Factor Binding Protein 2 (IGFBP2) in the Regulation of Corneal Fibroblast Differentiation
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Autocrine IFN Signaling Inducing Profibrotic Fibroblast Responses By
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Anti-IGFBP6 Antibody (ARG10677)
Product datasheet [email protected] ARG10677 Package: 50 μg anti-IGFBP6 antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes IGFBP6 Tested Reactivity Hu, Rat Predict Reactivity Ms Tested Application WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name IGFBP6 Antigen Species Human Immunogen Synthetic peptide corresponding to the sequence at a.a 189-205 (NCDHRGFYRKRQCRSSQ) around the middle region of human IGFBP6 protein. Conjugation Un-conjugated Alternate Names IGF-Binding Protein; IGFBP-6; IBP-6; IGF Binding Protein 6 Application Instructions Application table Application Dilution WB 0.1 - 0.5 μg/ml Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Calculated Mw 25 kDa Properties Form Liquid Purification Affinity purification with immunogen. Buffer 1X PBS, 0.025% Sodium azide and 2.8% BSA Preservative 0.028% Sodium azide Stabilizer 2.8% BSA Concentration 0.5 mg/ml Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Database links GeneID: 25641 Rat GeneID: 3489 Human Swiss-port # P24592 Human Swiss-port # P35572 Rat Gene Symbol IGFBP6 Gene Full Name Insulin Like Growth Factor Binding Protein 6 Background IGFBP6 (Insulin Like Growth Factor Binding Protein 6) is a Protein Coding gene.
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