A Study of the Soil-Plant Interactions of Pistacia Lentiscus L. Distributed in the Western Anatolian Part of Turkey
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Acta Bot. Croat. 62 (2), 73–88, 2003 CODEN: ABCRA25 ISSN 0365–0588 A study of the soil-plant interactions of Pistacia lentiscus L. distributed in the western Anatolian part of Turkey YUNUS DOGAN*, SULEYMAN BASLAR,HALIL AYDIN,HASAN HUSEYIN MERT Dokuz Eylül University, Faculty of Education, Dept. Biology, 35150 Buca Izmir, Turkey This study was undertaken with the aim of illuminating the soil-plant interactions of Pistacia lentiscus L., which is a Mediterranean sclerophylleous coastal zone plant in the Western Anatolian part of Turkey. The soil analysis data showed that this plant grows on different kinds of soils such as sandy-clayey-loam, clayey-loam, sandy-loam and loamy texture. Soils are not saline, with pH moderately and slightly alkaline. This species prefers soils with low phosphorus and potassium contents, but with different calcium carbonate and nitrogen contents. Three negative linear correlations were observed between plant calcium and soil pH, plant nitrogen and soil calcium carbonate, plant potassium and soil calcium carbonate. Key words: Pistacia lentiscus, autecology, Anatolia. Introduction Pistacia lentiscus L. (Anacardiaceae) is a small Mediterranean species, with a height of up to 8 m; it appears as an evergreen tree or shrub, distributed up to 800 m a.s.l. It appears in dry open woods and scrublands in the Mediterranean Region (extending to Portugal and Canary Islands), and in garrigues, maquis on sandy soils and dry rocky slopes. In Turkey, it is distributed throughout the Aegean and Mediterranean geographical regions (DAVIS 1966). P. lentiscus has an economic value as it is the source of a traditional medicinal agent "gum" mastic, an oleoresin traditionally used in the perfume industry and as a chewing gum in many cultures, and used in pharmaceuticals, dental adhesives, and in high grade varnishes for protecting pictures. Mastic is composed of resinous exudates obtained from the stem and the main leaves of P. lentiscus. It is used as a food ingredient in the Mediterra- nean region. It has been discovered that mastic gum has been proved to kill the carcino- genic bacteria Heliobacter pylori which is responsible for peptic ulcers, very effectively (AL-SAID et al. 1986, MARONE et al. 2001). Therefore, clinically, mastic has been effective in the treatment of benign gastric ulcers (HUWEZ and AL-HABBAL 1986) and duodenal ul- cers (AL-HABBAL et al. 1984). * Corresponding author: Fax: 0090 232 420 48 95, e-mail: [email protected] ACTA BOT. CROAT. 62 (2), 2003 73 DOGAN Y., BASLAR S., AYDIN H., MERT H. H. There have been various studies of P. lentiscus, especially after the discovery of the antimicrobial activity of its essential oils (TASSOU and NYCHAS 1995, IAUK et al. 1996, ALI-SHTAYEH and ABU GHDEIB 1999). Methanol extracts of P. lentiscus and Thymus maroccanus were very active against the virus Herpes simplex (MOUHAJIR et al. 2001). There are also some studies about insecticidal effects of this species (TRABOULSI et al. 2002). The oils of P.lentiscus were more efficient against the eggs of the pest (LAMIRI et al. 2001). Insecticidal effects were detected when extracts of P. lentiscus, Genista umbellata and Cachrys sicula where mixed with the insect diet (PASCUAL-VILLALOBOS and ROB- LEDO 1998). There are also some studies about the chemical characteristics of this species. Young leaves of P. lentiscus were richer in nitrogen and tannins but poorer in carbohydrate con- tents, as compared to mature leaves (MARAKIS and DIAMANTOGLOU 1990). The essential oils of individual plants of P. lentiscus from Corsica, such as myrcene, limonene, terpinen- -4-ol, alpha-pinene, beta-pinene, alpha-phellandrene, sabinene, p-cymene and gamma-ter- pinene were found to be the main constituents (CASTOLA et al. 2000). SAWIDIS et al. (2000) investigated the distribution, anatomy and development of the resin duct system responsible for the resin exudation in P. lentiscus var. chia. They found that resin ducts were present in the stem, leaf and root located only in the phloem of the vas- cular bundles. Pistacia lentiscus is well adapted to the semiarid Mediterranean basin. However, it is negatively affected by tourism, fire, degradation and deforestation of the region for planta- tion (especially for olive tree plantation) or for making secondary houses, etc. As a result of this pressure on the species, the number of individuals representing the species in the re- gion has been reduced. To our knowledge, no detailed study of the soil and plant character- istics of P. lentiscus in our study area has been published. The aim of this study, then, is to find out some of the physical and chemical characteristics of soils supporting this species in the coastal zones of Western Anatolia, some of the chemical characteristics of the aerial parts of the plant, with materials collected from the study area, and the statistical relations between results of the analysis of soil and plant samples. Investigated area This study was carried out in the vicinity of five cities (Canakkale, Balikesir, Izmir, Aydin and Mugla) in the coastal region of West Anatolia in Turkey (Fig. 1). In West Anatolia, different geological and lithological structures can be seen. The structure of the study area, generally, is Palaeozoic metamorphic schist-gneiss, mica schist; alluvion, qua- ternary; Neogene marl, sand stone, soft limestone; Mesozoic limestone, flysch and ophio- lite. Related to this structure, soils of the region, generally, are red Mediterranean soil- -Alfisol, brown forest soil– Inceptisol, and alluvial soil (ATALAY 1994). Meteorological data about drought level and other climatic conditions of the study area were obtained from the local meteorology stations of the five cities, and EMBERGER’s formula was applied (NAHAL 1981) (Tab. 1). In EMBERGER’s climate classification, the following climatic elements are used, taking into consideration the fact that plants are ac- tive between certain temperatures: the mean minimum temperature for the coldest month 74 ACTA BOT. CROAT. 62 (2), 2003 PISTACIA LENTISCUS IN WEST ANATOLIA Fig. 1. Map showing the plant and soil sample collection localities from West Anatolia. Tab. 1. Meteorogical data obtained from the local meteorology stations of cities. P: annual precip- itation, M: the mean maximum temperature for the hottest month, m: the mean minimum temperature for the coldest month, and Q: pluviothermic quotient values. City P (mm) M (°C) m (°C) Q Canakkale 628.5 30.2 2.8 78.2 Balikesir 594.8 30.7 1.5 70.4 Izmir 695.2 32.7 5.5 87 Aydin 670.1 35.1 4.2 73 Mugla 1209.2 32.8 1.6 133.5 ACTA BOT. CROAT. 62 (2), 2003 75 DOGAN Y., BASLAR S., AYDIN H., MERT H. H. (m), the mean maximum temperature for the hottest month (M), annual precipitation (P) and pluviothermic quotient values (Q). The study area is classified into humid and sub-hu- mid bioclimatic zones, among six Mediterranean bioclimatic zones. Material and methods Collection of specimens Plant specimens of P.lentiscus were collected at 20 localities along the West Anatolia in June 2000 (Fig. 1): Izmir: 1. Gumuldur-Yenikoy, 2. Bornova-Ciceklikoyu, 3. Seferihisar-Akkum, 4. Cesmealti-Guvendik, 5. Karaburun-Mordogan, 6. Cesme, 7. Aliaga. Aydin: 8. Didim-Akbuk, 9. Ortaklar, 10. Pamucak, 11. Davutlar-National Park, 12. Soke. Balikesir: 13. Burhaniye, 14. Ayvalik, 15. Altinoluk. Mugla: 16. Milas, 17. Bodrum-Gundogan, 18. Fethiye, 19. Marmaris-Ilicalar. Canakkale: 20. Ezine-Geyikli. Collected plants were identified according to DAVIS (1966). All the specimens were stored in a personal herbarium. For chemical analysis, the above-ground parts (stem, shoots, leaves, flowers) of the specimens were also collected. In this collection procedure, in each locality three sets of samples – at least one branch from top and bottom, and from four sides of the middle of the tree – each totally containing six branches, are collected. They are then, dried in an oven at 80 °C for 24 hours and milled. Soil samples of P. lentiscus are taken from the same localities where the plant samples are collected. The litter on the surface of the soil is removed, and soil samples are collected from a depth of 15–20 cm, put into polyethylene bags and brought to the laboratory. They are left under laboratory conditions for air-drying. They are then passed through a 2 mm sieve and stored for analysis. Physical chemical analysis Total nitrogen in the soil and the plant is determined according to BREMNER (1965) by using the Kjeldahl method, phosphorus in the soil is determined according to BINGHAM (1949) and in the plant according to LOTT et al. (1956), using a Spectrum 2000 Spectro- photometer, potassium and calcium in the soil and the plant are determined according to PRATT (1965), using a Jenway Flame Photometer. Each measurement is repeated three times and the mean values of these measurements are presented as a result. Data analysis Regression models were developed for each effective factor through which the relation- ship between each factor of the soils (values obtained in this study about pH, calcium car- bonate, total soluble salts, nitrogen, phosphorus and potassium) and the aerial parts of plant samples (nitrogen, phosphorus, potassium and calcium) are investigated. The estimators in the models are tested by t -test and the overall regression model by F-test at the significant level of 0.10. Moreover, R’s of the models are discussed. All the regression models are ob- tained in the computer by using SPSS (Statistical Package for Social Sciences) statistical package. 76 ACTA BOT. CROAT. 62 (2), 2003 PISTACIA LENTISCUS IN WEST ANATOLIA Results Pistacia lentiscus grows on sandy-clayey-loam, sandy-loam, clayey-loam and loamy soils. The pH, total soluble salts, calcium carbonate, nitrogen, phosphorus and potassium values of our soil samples range between 7.90–8.06, 0.030–0.111%, 1.220–40.800%, 0.042–2.114%, 0.00002–0.00050% and 0.020–0.074%, respectively (Tabs.