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Unfolding the Secrets of Coral–Algal Symbiosis
The ISME Journal (2015) 9, 844–856 & 2015 International Society for Microbial Ecology All rights reserved 1751-7362/15 www.nature.com/ismej ORIGINAL ARTICLE Unfolding the secrets of coral–algal symbiosis Nedeljka Rosic1, Edmund Yew Siang Ling2, Chon-Kit Kenneth Chan3, Hong Ching Lee4, Paulina Kaniewska1,5,DavidEdwards3,6,7,SophieDove1,8 and Ove Hoegh-Guldberg1,8,9 1School of Biological Sciences, The University of Queensland, St Lucia, Queensland, Australia; 2University of Queensland Centre for Clinical Research, The University of Queensland, Herston, Queensland, Australia; 3School of Agriculture and Food Sciences, The University of Queensland, St Lucia, Queensland, Australia; 4The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, New South Wales, Australia; 5Australian Institute of Marine Science, Townsville, Queensland, Australia; 6School of Plant Biology, University of Western Australia, Perth, Western Australia, Australia; 7Australian Centre for Plant Functional Genomics, The University of Queensland, St Lucia, Queensland, Australia; 8ARC Centre of Excellence for Coral Reef Studies, The University of Queensland, St Lucia, Queensland, Australia and 9Global Change Institute and ARC Centre of Excellence for Coral Reef Studies, The University of Queensland, St Lucia, Queensland, Australia Dinoflagellates from the genus Symbiodinium form a mutualistic symbiotic relationship with reef- building corals. Here we applied massively parallel Illumina sequencing to assess genetic similarity and diversity among four phylogenetically diverse dinoflagellate clades (A, B, C and D) that are commonly associated with corals. We obtained more than 30 000 predicted genes for each Symbiodinium clade, with a majority of the aligned transcripts corresponding to sequence data sets of symbiotic dinoflagellates and o2% of sequences having bacterial or other foreign origin. -
Growth and Grazing Rates of the Herbivorous Dinoflagellate Gymnodinium Sp
MARINE ECOLOGY PROGRESS SERIES Published December 16 Mar. Ecol. Prog. Ser. Growth and grazing rates of the herbivorous dinoflagellate Gymnodinium sp. from the open subarctic Pacific Ocean Suzanne L. Strom' School of Oceanography WB-10, University of Washington. Seattle. Washington 98195, USA ABSTRACT: Growth, grazing and cell volume of the small heterotroph~cdinoflagellate Gyrnnodin~um sp. Isolated from the open subarctic Pacific Ocean were measured as a funct~onof food concentration using 2 phytoplankton food species. Growth and lngestlon rates increased asymptotically with Increas- ing phytoplankon food levels, as did grazer cell volume; rates at representative oceanic food levels were high but below maxima. Clearance rates decreased with lncreaslng food levels when Isochrysis galbana was the food source; they increased ~vithlncreaslng food levels when Synechococcus sp. was the food source. There was apparently a grazlng threshold for Ingestion of Synechococcus: below an initial Synechococcus concentration of 20 pgC 1.' ingestion rates on this alga were very low, while above this initial concentratlon Synechococcus was grazed preferent~ally Gross growth efficiency varied between 0.03 and 0.53 (mean 0.21) and was highest at low food concentrations. Results support the hypothesis that heterotrophic d~noflagellatesmay contribute to controlling population increases of small, rap~dly-grow~ngphytoplankton specles even at low oceanic phytoplankton concentrations. INTRODUCTION as Gymnodinium and Gyrodinium is difficult or impos- sible using older preservation and microscopy tech- Heterotrophic dinoflagellates can be a significant niques; experimental emphasis has been on more component of the microzooplankton in marine waters. easily recognizable and collectable microzooplankton In the oceanic realm, Lessard (1984) and Shapiro et al. -
The Planktonic Protist Interactome: Where Do We Stand After a Century of Research?
bioRxiv preprint doi: https://doi.org/10.1101/587352; this version posted May 2, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Bjorbækmo et al., 23.03.2019 – preprint copy - BioRxiv The planktonic protist interactome: where do we stand after a century of research? Marit F. Markussen Bjorbækmo1*, Andreas Evenstad1* and Line Lieblein Røsæg1*, Anders K. Krabberød1**, and Ramiro Logares2,1** 1 University of Oslo, Department of Biosciences, Section for Genetics and Evolutionary Biology (Evogene), Blindernv. 31, N- 0316 Oslo, Norway 2 Institut de Ciències del Mar (CSIC), Passeig Marítim de la Barceloneta, 37-49, ES-08003, Barcelona, Catalonia, Spain * The three authors contributed equally ** Corresponding authors: Ramiro Logares: Institute of Marine Sciences (ICM-CSIC), Passeig Marítim de la Barceloneta 37-49, 08003, Barcelona, Catalonia, Spain. Phone: 34-93-2309500; Fax: 34-93-2309555. [email protected] Anders K. Krabberød: University of Oslo, Department of Biosciences, Section for Genetics and Evolutionary Biology (Evogene), Blindernv. 31, N-0316 Oslo, Norway. Phone +47 22845986, Fax: +47 22854726. [email protected] Abstract Microbial interactions are crucial for Earth ecosystem function, yet our knowledge about them is limited and has so far mainly existed as scattered records. Here, we have surveyed the literature involving planktonic protist interactions and gathered the information in a manually curated Protist Interaction DAtabase (PIDA). In total, we have registered ~2,500 ecological interactions from ~500 publications, spanning the last 150 years. -
Seasonal Dynamics of Meroplankton in a High-Latitude Fjord
1 Seasonal dynamics of meroplankton in a high-latitude fjord 2 3 Helena Kling Michelsen*1, Camilla Svensen1, Marit Reigstad1, Einar Magnus Nilssen1, 4 Torstein Pedersen1 5 6 1Department of Arctic and Marine Biology, UiT the Arctic University of Norway, Tromsø, 7 Norway 8 9 * Corresponding author: [email protected] 10 Faculty of Biosciences, Fisheries and Economics, 11 Department of Arctic and Marine Biology, 12 UiT the Arctic University of Norway, 9037 Tromsø, Norway. 1 13 Abstract 14 Knowledge on the seasonal timing and composition of pelagic larvae of many benthic 15 invertebrates, referred to as meroplankton, is limited for high-latitude fjords and coastal areas. 16 We investigated the seasonal dynamics of meroplankton in the sub-Arctic Porsangerfjord 17 (70˚N), Norway, by examining their seasonal changes in relation to temperature, chlorophyll 18 a and salinity. Samples were collected at two stations between February 2013 and August 19 2014. We identified 41 meroplanktonic taxa from eight phyla. Multivariate analysis indicated 20 different meroplankton compositions in winter, spring, early summer and late summer. More 21 larvae appeared during spring and summer, forming two peaks in meroplankton abundance. 22 The spring peak was dominated by cirripede nauplii, and late summer peak was dominated by 23 bivalve veligers. Moreover, spring meroplankton were the dominant component in the 24 zooplankton community this season. In winter, low abundances and few meroplanktonic taxa 25 were observed. Timing for a majority of meroplankton correlated with primary production 26 and temperature. The presence of meroplankton in the water column through the whole year 27 and at times dominant in the zooplankton community, suggests that they, in addition to being 28 important for benthic recruitment, may play a role in the pelagic ecosystem as grazers on 29 phytoplankton and as prey for other organisms. -
(Alveolata) As Inferred from Hsp90 and Actin Phylogenies1
J. Phycol. 40, 341–350 (2004) r 2004 Phycological Society of America DOI: 10.1111/j.1529-8817.2004.03129.x EARLY EVOLUTIONARY HISTORY OF DINOFLAGELLATES AND APICOMPLEXANS (ALVEOLATA) AS INFERRED FROM HSP90 AND ACTIN PHYLOGENIES1 Brian S. Leander2 and Patrick J. Keeling Canadian Institute for Advanced Research, Program in Evolutionary Biology, Departments of Botany and Zoology, University of British Columbia, Vancouver, British Columbia, Canada Three extremely diverse groups of unicellular The Alveolata is one of the most biologically diverse eukaryotes comprise the Alveolata: ciliates, dino- supergroups of eukaryotic microorganisms, consisting flagellates, and apicomplexans. The vast phenotypic of ciliates, dinoflagellates, apicomplexans, and several distances between the three groups along with the minor lineages. Although molecular phylogenies un- enigmatic distribution of plastids and the economic equivocally support the monophyly of alveolates, and medical importance of several representative members of the group share only a few derived species (e.g. Plasmodium, Toxoplasma, Perkinsus, and morphological features, such as distinctive patterns of Pfiesteria) have stimulated a great deal of specula- cortical vesicles (syn. alveoli or amphiesmal vesicles) tion on the early evolutionary history of alveolates. subtending the plasma membrane and presumptive A robust phylogenetic framework for alveolate pinocytotic structures, called ‘‘micropores’’ (Cavalier- diversity will provide the context necessary for Smith 1993, Siddall et al. 1997, Patterson -
Short Term in Vitro Culture of Cryptocaryon Irritans, a Protozoan Parasite of Marine Fishes
魚 病 研 究 Fish Pathology,39(4),175-181,2004.12 2004 The Japanese Society of Fish Pathology Short Term in vitro Culture of Cryptocaryon irritans, a Protozoan Parasite of Marine Fishes Apolinario V. Yambot1,3 and Yen-Ling Song1,2* 1Institute of Zoology, National Taiwan University, Taipei 106, Taiwan, ROC 2Department of Life Science , National Taiwan University, Taipei 106, Taiwan, ROC3 Present address: College of Fisheries-Freshwater Aquaculture Center , Central Luzon State University, Philippines (Received March 19, 2004) ABSTRACT--Attempts were made to cultivate Cryptocaryon irritans in vitro at 23-25℃. Attachment of theronts and subsequent enlargement into trophonts were achieved in two experi ments using strips of trypticase soy agar (TSA, supplemented with 3% NaCl) as an attachment substrate in filtered seawater. In the third experiment, transformation of theronts into trophonts was achieved in an enriched liquid medium composed of 50% filtered seawater, 30% Leibovitz L-15 and 20% fetal calf serum without attachment onto the TSA. Sizes (mean ±SD) of the trophonts, 114.6 ± 57.9 μm to 295.9 ± 130 μm, were from a recorded size range (50 to 700 μm) of the parasite in vivo. Although only limited numbers of theronts (0.28-1.71%) transformed into trophonts, these results showed that the in vitro culture of C. irritans is potentially feasible as evidenced by the enlargement of the trophonts within the in vivo size range using either a solid medium as an attach ment substrate or a liquid medium without attachment. There is a need, however, to determine essential factors that influence the transformation of the trophonts into viable tomonts capable of producing theronts. -
Zooplankton Chapters 6-8 in Miller for More Details 1. Crustaceans- Include Shrimp, Copepods, Euphausiids ("Krill") 2
Ocean Processes and Ecology Spring 2004 Zooplankton Chapters 6-8 in Miller for more details I. Major Groups Heterotrophs— consume organic matter rather than manufacturing it, as do autotrophs. Zooplankton can be: herbivores carnivores (several levels) detritus feeders omnivores Zooplankton, in addition to being much smaller than familiar land animals, have shorter generation times and grow more rapidly (in terms of % of body wt / day). 1. Crustaceans- include shrimp, copepods, euphausiids ("krill") Characteristics: Copepods, euphausiids and shrimp superficially resemble one another. All have: • exoskeletons of chitin • jointed appendages • 2 pair of antennae • complex body structure, with well developed internal organs and sensory organs Habitats: Ubiquitous. • Euphausiids predominate in the Antarctic Ocean, but are common in most temperate and polar oceans. • Copepods are found everywhere, but are less important in low-productivity areas of the ocean - the "central ocean gyres". They are found at all depths but are more abundant near the surface. Role in food webs: • Euphausiids and copepods are filter-feeders. Copepods are usually herbivores, while the larger euphausiids consume both phytoplankton and other zooplankton. • Shrimp are usually carnivores or scavengers. 2. Chaetognaths - ("Arrow worms") Characteristics: • 2-3 cm long • wormlike, but non-segmented • no appendages (legs or antennae) • complex body structure with internal organs Habitat: Ubiquitous Ocean Processes and Ecology Spring 2004 Role in food web: Carnivore feeding on small zooplankton such as copepods. 3. Protozoan - Include foraminifera, radiolarians, tintinnids and "microflagellates" ca. 0.002 mm Characteristics: • Single-celled animals. • Forams have calcareous shell • Radiolarians have siliceous shell. • Both Forams and Radiolarians have spines. Habitat: Ubiquitous • Radiolarians are especially abundant in the Pacific equatorial upwelling region. -
Biological Oceanography - Legendre, Louis and Rassoulzadegan, Fereidoun
OCEANOGRAPHY – Vol.II - Biological Oceanography - Legendre, Louis and Rassoulzadegan, Fereidoun BIOLOGICAL OCEANOGRAPHY Legendre, Louis and Rassoulzadegan, Fereidoun Laboratoire d'Océanographie de Villefranche, France. Keywords: Algae, allochthonous nutrient, aphotic zone, autochthonous nutrient, Auxotrophs, bacteria, bacterioplankton, benthos, carbon dioxide, carnivory, chelator, chemoautotrophs, ciliates, coastal eutrophication, coccolithophores, convection, crustaceans, cyanobacteria, detritus, diatoms, dinoflagellates, disphotic zone, dissolved organic carbon (DOC), dissolved organic matter (DOM), ecosystem, eukaryotes, euphotic zone, eutrophic, excretion, exoenzymes, exudation, fecal pellet, femtoplankton, fish, fish lavae, flagellates, food web, foraminifers, fungi, harmful algal blooms (HABs), herbivorous food web, herbivory, heterotrophs, holoplankton, ichthyoplankton, irradiance, labile, large planktonic microphages, lysis, macroplankton, marine snow, megaplankton, meroplankton, mesoplankton, metazoan, metazooplankton, microbial food web, microbial loop, microheterotrophs, microplankton, mixotrophs, mollusks, multivorous food web, mutualism, mycoplankton, nanoplankton, nekton, net community production (NCP), neuston, new production, nutrient limitation, nutrient (macro-, micro-, inorganic, organic), oligotrophic, omnivory, osmotrophs, particulate organic carbon (POC), particulate organic matter (POM), pelagic, phagocytosis, phagotrophs, photoautotorphs, photosynthesis, phytoplankton, phytoplankton bloom, picoplankton, plankton, -
Seasonal Variation of the Sound-Scattering Zooplankton Vertical Distribution in the Oxygen-Deficient Waters of the NE Black
Ocean Sci., 17, 953–974, 2021 https://doi.org/10.5194/os-17-953-2021 © Author(s) 2021. This work is distributed under the Creative Commons Attribution 4.0 License. Seasonal variation of the sound-scattering zooplankton vertical distribution in the oxygen-deficient waters of the NE Black Sea Alexander G. Ostrovskii, Elena G. Arashkevich, Vladimir A. Solovyev, and Dmitry A. Shvoev Shirshov Institute of Oceanology, Russian Academy of Sciences, 36, Nakhimovsky prospekt, Moscow, 117997, Russia Correspondence: Alexander G. Ostrovskii ([email protected]) Received: 10 November 2020 – Discussion started: 8 December 2020 Revised: 22 June 2021 – Accepted: 23 June 2021 – Published: 23 July 2021 Abstract. At the northeastern Black Sea research site, obser- layers is important for understanding biogeochemical pro- vations from 2010–2020 allowed us to study the dynamics cesses in oxygen-deficient waters. and evolution of the vertical distribution of mesozooplank- ton in oxygen-deficient conditions via analysis of sound- scattering layers associated with dominant zooplankton ag- gregations. The data were obtained with profiler mooring and 1 Introduction zooplankton net sampling. The profiler was equipped with an acoustic Doppler current meter, a conductivity–temperature– The main distinguishing feature of the Black Sea environ- depth probe, and fast sensors for the concentration of dis- ment is its oxygen stratification with an oxygenated upper solved oxygen [O2]. The acoustic instrument conducted ul- layer 80–200 m thick and the underlying waters contain- trasound (2 MHz) backscatter measurements at three angles ing hydrogen sulfide (Andrusov, 1890; see also review by while being carried by the profiler through the oxic zone. For Oguz et al., 2006). -
Mixotrophic Protists Among Marine Ciliates and Dinoflagellates: Distribution, Physiology and Ecology
FACULTY OF SCIENCE UNIVERSITY OF COPENHAGEN PhD thesis Woraporn Tarangkoon Mixotrophic Protists among Marine Ciliates and Dinoflagellates: Distribution, Physiology and Ecology Academic advisor: Associate Professor Per Juel Hansen Submitted: 29/04/10 Contents List of publications 3 Preface 4 Summary 6 Sammenfating (Danish summary) 8 สรุป (Thai summary) 10 The sections and objectives of the thesis 12 Introduction 14 1) Mixotrophy among marine planktonic protists 14 1.1) The role of light, food concentration and nutrients for 17 the growth of marine mixotrophic planktonic protists 1.2) Importance of marine mixotrophic protists in the 20 planktonic food web 2) Marine symbiont-bearing dinoflagellates 24 2.1) Occurrence of symbionts in the order Dinophysiales 24 2.2) The spatial distribution of symbiont-bearing dinoflagellates in 27 marine waters 2.3) The role of symbionts and phagotrophy in dinoflagellates with symbionts 28 3) Symbiosis and mixotrophy in the marine ciliate genus Mesodinium 30 3.1) Occurrence of symbiosis in Mesodinium spp. 30 3.2) The distribution of marine Mesodinium spp. 30 3.3) The role of symbionts and phagotrophy in marine Mesodinium rubrum 33 and Mesodinium pulex Conclusion and future perspectives 36 References 38 Paper I Paper II Paper III Appendix-Paper IV Appendix-I Lists of publications The thesis consists of the following papers, referred to in the synthesis by their roman numerals. Co-author statements are attached to the thesis (Appendix-I). Paper I Tarangkoon W, Hansen G Hansen PJ (2010) Spatial distribution of symbiont-bearing dinoflagellates in the Indian Ocean in relation to oceanographic regimes. Aquat Microb Ecol 58:197-213. -
Is Chloroplastic Class IIA Aldolase a Marine Enzyme&Quest;
The ISME Journal (2016) 10, 2767–2772 © 2016 International Society for Microbial Ecology All rights reserved 1751-7362/16 www.nature.com/ismej SHORT COMMUNICATION Is chloroplastic class IIA aldolase a marine enzyme? Hitoshi Miyasaka1, Takeru Ogata1, Satoshi Tanaka2, Takeshi Ohama3, Sanae Kano4, Fujiwara Kazuhiro4,7, Shuhei Hayashi1, Shinjiro Yamamoto1, Hiro Takahashi5, Hideyuki Matsuura6 and Kazumasa Hirata6 1Department of Applied Life Science, Sojo University, Kumamoto, Japan; 2The Kansai Electric Power Co., Environmental Research Center, Keihanna-Plaza, Kyoto, Japan; 3School of Environmental Science and Engineering, Kochi University of Technology, Kochi, Japan; 4Chugai Technos Corporation, Hiroshima, Japan; 5Graduate School of Horticulture, Faculty of Horticulture, Chiba University, Chiba, Japan and 6Environmental Biotechnology Laboratory, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan Expressed sequence tag analyses revealed that two marine Chlorophyceae green algae, Chlamydo- monas sp. W80 and Chlamydomonas sp. HS5, contain genes coding for chloroplastic class IIA aldolase (fructose-1, 6-bisphosphate aldolase: FBA). These genes show robust monophyly with those of the marine Prasinophyceae algae genera Micromonas, Ostreococcus and Bathycoccus, indicating that the acquisition of this gene through horizontal gene transfer by an ancestor of the green algal lineage occurred prior to the divergence of the core chlorophytes (Chlorophyceae and Treboux- iophyceae) and the prasinophytes. The absence of this gene in some freshwater chlorophytes, such as Chlamydomonas reinhardtii, Volvox carteri, Chlorella vulgaris, Chlorella variabilis and Coccomyxa subellipsoidea, can therefore be explained by the loss of this gene somewhere in the evolutionary process. Our survey on the distribution of this gene in genomic and transcriptome databases suggests that this gene occurs almost exclusively in marine algae, with a few exceptions, and as such, we propose that chloroplastic class IIA FBA is a marine environment-adapted enzyme. -
CH28 PROTISTS.Pptx
9/29/14 Biosc 41 Announcements 9/29 Review: History of Life v Quick review followed by lecture quiz (history & v How long ago is Earth thought to have formed? phylogeny) v What is thought to have been the first genetic material? v Lecture: Protists v Are we tetrapods? v Lab: Protozoa (animal-like protists) v Most atmospheric oxygen comes from photosynthesis v Lab exam 1 is Wed! (does not cover today’s lab) § Since many of the first organisms were photosynthetic (i.e. cyanobacteria), a LOT of excess oxygen accumulated (O2 revolution) § Some organisms adapted to use it (aerobic respiration) Review: History of Life Review: Phylogeny v Which organelles are thought to have originated as v Homology is similarity due to shared ancestry endosymbionts? v Analogy is similarity due to convergent evolution v During what event did fossils resembling modern taxa suddenly appear en masse? v A valid clade is monophyletic, meaning it consists of the ancestor taxon and all its descendants v How many mass extinctions seem to have occurred during v A paraphyletic grouping consists of an ancestral species and Earth’s history? Describe one? some, but not all, of the descendants v When is adaptive radiation likely to occur? v A polyphyletic grouping includes distantly related species but does not include their most recent common ancestor v Maximum parsimony assumes the tree requiring the fewest evolutionary events is most likely Quiz 3 (History and Phylogeny) BIOSC 041 1. How long ago is Earth thought to have formed? 2. Why might many organisms have evolved to use aerobic respiration? PROTISTS! Reference: Chapter 28 3.