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Hearing in Butterflies: Neurophysiological Characterization of the Auditory Afferents in Morpho Peleides (Nymphalidae)

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Hearing in Butterflies: Neurophysiological Characterization of the Auditory Afferents in Morpho peleides (Nymphalidae) By Andrew Mikhail A dissertation submitted to the Faculty of Graduate Studies and Research in partial fulfillment of the requirements for the degree of Master of Science in Biology Carleton University Ottawa, Ontario © 2014 Andrew Mikhail Abstract Many butterflies have well developed tympanal ears on their wings but little is known about what they are capable of hearing in their natural environments. The tympanal ear of many butterflies, including Morpho peleides, comprises an outer membrane and a conspicuous inner dome (tholus), both of which are innervated by two separate auditory nerve branches (NII and NIII) and their respective sensory organs. Using extracellular neurophysiological recordings, I explored how this morphology contributes to mechanical sound frequency and amplitude discrimination. I also show that the auditory nerves of M. peleides responded to playbacks of the broadband cyclic sounds produced passively during flight of blue jays (Cyanocitta cristata), as well as to the low frequency vocalizations of one of its main avian predators, the rufous-tailed jacamar (Galbula ruficauda), providing further evidence that butterflies and possibly other diurnal insects could be using their sense of hearing to detect and avoid avian predators. ii Acknowledgments Special thanks to my supervisor Dr. Jayne Yack who was always supportive from day one of my graduate studies. Dr. Yack’s constant encouragement and enthusiasm is the reason I spent so many hours in the dark basement laboratory of the Nesbitt building. Despite her busy schedule, she always seemed to find time to meet with me, and she encouraged me to publish and present my research at conferences, both locally and internationally. My co-supervisor Dr. John Lewis has been instrumental in the completion of this research. His expertise with data analysis and his knowledge of MATLAB was truly invaluable. I’d also like to thank Dr. Jeff Dawson, a member of my graduate committee and a long time mentor. Dr. Dawson was a tremendous help in setting up and troubleshooting the recording equipment. I shared my workspace with amazing students that also deserve to be thanked. Laura McMillan, Amanda Lindeman, and Christian Nathan have all encouraged me throughout the way and have definitely made my time in the Yack laboratory much more enjoyable. Finally, I would also like to thank Edward Bruggink for allowing me to use his greenhouse nursery and for supporting the care for the butterflies. Funding for this work was provided to Dr. Jayne Yack by the Natural Sciences and Engineering Research Council (NSERC), the Canadian Foundation for Innovation (CFI), the Ontario Innovation Trust, and the Early Researcher Award (ERA). Funding to Andrew Mikhail was provided by Carleton University and NSERC PGSM. iii Table of Contents Abstract .............................................................................................................................. ii Acknowledgments ............................................................................................................ iii Table of Contents ............................................................................................................. iv List of Tables ..................................................................................................................... v List of Illustrations ........................................................................................................... vi List of Abbreviations ...................................................................................................... vii Section 1: General introduction: Hearing in butterflies ............................................... 1 Insect hearing .................................................................................................................. 2 Hearing in butterflies ...................................................................................................... 4 Study species – Morpho peleides .................................................................................... 8 Thesis objectives ........................................................................................................... 11 Main objective: ..........................................................................................................11 Specific objectives: ....................................................................................................11 Study significance: ........................................................................................................ 17 Section 2: Analysis of the neural auditory responses of the tropical butterfly Morpho peleides (Nymphalidae) and a test of the avian hypothesis ......................................... 18 Statement of Contributions ........................................................................................... 20 Abstract ......................................................................................................................... 21 Abbreviations ................................................................................................................ 22 Key words ..................................................................................................................... 22 Introduction ................................................................................................................... 23 Materials and methods .................................................................................................. 28 Animals ......................................................................................................................28 Neurophysiology ........................................................................................................28 Sound stimuli .............................................................................................................29 Audiograms ................................................................................................................29 Intensity ramps ...........................................................................................................30 Response measurements ............................................................................................30 Playback of predatory bird sounds .............................................................................31 Results ........................................................................................................................... 34 Threshold responses ...................................................................................................34 Supra-threshold responses .........................................................................................37 Vibration – Physiology results ...................................................................................39 Responses to avian flight sounds ...............................................................................41 Responses to avian vocalizations ...............................................................................44 Discussion ..................................................................................................................... 46 Acknowledgements ....................................................................................................... 53 Funding ......................................................................................................................... 54 Section 3: General conclusion ........................................................................................ 55 Future directions ........................................................................................................... 58 Section 4: References ...................................................................................................... 60 Section 5: Appendix ........................................................................................................ 66 iv List of Tables Table 2.1 Statistical comparisons (Mann-Whitney U-tests) of the audiograms from the two nerve branches NII and NIII in Morpho peleides.........................36 v List of Illustrations Fig 2.1 Location and morphology of the Vogel’s Organ (VO) on the butterfly Morpho peleides............................................................................................................ 27 Fig 2.2 Typical compound action potential (CAP)...................................................... 33 Fig 2.3 Auditory tuning curves for Morpho peleides................................................... 35 Fig 2.4 Extracellular responses from NII and NIII in Morpho peleides...................... 38 Fig 2.5 Vibrational characteristics of the tympanal membrane and neuro-physiological data of both auditory nerve branches............................................................... 40 Fig 2.6 Auditory nerve responses of NII to blue jay bird flight sounds....................... 42 Fig 2.7 Auditory nerve responses of NIII to blue jay bird flight sounds..................... 43 Fig 2.8 Auditory nerve responses of NIII to jacamar vocalizations............................. 45 Fig 5.1 Neural response of an underwing moth (Noctuoidea) to various amplitudes of the broadband flight sounds of an eastern phoebe (Sayornis phoebe)............................... 66 vi List of Abbreviations LV: Laser Vibrometry VO: Vogel’s Organ CAP:
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