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Chemiluminescence Western Blotting Technical Guide and Protocols
TECH TIP Chemiluminescent substrates Chemiluminescence western blotting technical guide and protocols Introduction Types and considerations of chemiluminescence Western blotting is a powerful and commonly used tool western blotting to identify and quantify a specific protein in a complex HRP is the most popular enzyme used in western mixture. As originally conceived by Towbin et al., the blotting and will be discussed throughout this document technique enables indirect detection of protein samples as our example. The most suitable western blotting immobilized on a nitrocellulose or polyvinylidene fluoride substrates for HRP are luminol-based, and they produce (PVDF) membrane. In a conventional western blot, a chemiluminescent signal. Chemiluminescence is a protein samples are first resolved by sodium dodecyl chemical reaction that produces energy released in the sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) form of light. In the presence of HRP and a peroxide buffer, and then electrophoretically transferred to the membrane. luminol oxidizes and forms an excited-state product that Following a blocking step, the membrane is probed emits light as it decays to the ground state. Light emission with a primary antibody (polyclonal or monoclonal) occurs only during the enzyme–substrate reaction and, that was raised against the antigen in question. After a therefore, once the substrate in proximity to the enzyme is subsequent washing step, the membrane is incubated with exhausted, signal output ceases. In contrast, colorimetric a secondary antibody conjugated to an enzyme that is substrates, such as diaminobenzidine (DAB), produce reactive toward the primary antibody. An enzyme such as precipitate that remains visible on the membrane even alkaline phosphatase (AP) or horseradish peroxidase (HRP) after the reaction has terminated. -
Chem 257 Lecture for Exp. 8, 2004 1. Exp. 7 Report: to Be Handed in This Week, Along With: • Relevant Notebook Pages • Vial
Chem 257 Lecture for Exp. 8, 2004 1. Exp. 7 Report: To be handed in this week, along with: • Relevant notebook pages • Vial containing triphenyl carbinol labeled with your notebook code, i.e., JST-I-38a 2. Exp. 8 Quiz. Be prepared for Quiz. 3. Required Reading. Lab Manual 8.1-10. 4. Relevant Material in Wade (5th): Ultraviolet spectroscopy 15-13 (p 666-672), 16-15 p(711-713), 18-5E (p 785-6). Friedel Crafts Alkylation: 17-11 (p 746-748) & p 980. Exp. 8. Fluorescence and Chemiluminescence. Purpose of the experiment: 1. Synthetic Organic Chemistry • Acquaint you with Friedel-Crafts acylation chemistry 2. Physical Organic Chemistry • Introduce you to photophysics • Acquaint you with chemiluminescent reactions 1 Photochemical Terms and Principles. Useful to consider a ground and excited state energy diagram for an organic molecule. Energy Level Diagram ISC S = singlet S T = triplet 1 A = Absorption F = Fluorescence T IC 1 P = Phosphorescence A ISC = Intersystem crossing E F P IC = Internal Conversion ISC Absorption of a photon Emmission of a photon Vibrational energy loss So Singlet State (S) • Spin paired. • Ground state usually a singlet (So). • First excited singlet state = S1 So S1 • Nth excited singlet state = S n ground excited Triplet State (T) • Spin unpaired. • Lowest triplet state is usually an excited state (T1). • Nth excited triplet state = Tn T T Exceptions: 0 1 • Oxygen is a ground state Ground excited triplet! (O2) 2 Selection rules. • Spin allowed transitions do not involve a change in spin state (Sn → Sm, Tn → Tm). • Thus ground singlet states are most easily excited to their first excited singlet states (So → S1). -
Newsletter January/February 2020
NEW MEXICO GUN COLLECTOR’S ASSOCIATION Newsletter January/February 2020 NMGCA 66th ANNIVERSARY SPRING GUN, SWORD & KNIFE SHOW March 7 & 8, 2020 New Mexico State Fairgrounds Creative Arts Building (EXPO New Mexico) ALBUQUERQUE OLD WEST & MILITARY COLLECTIBLE SHOW August 1 & 2 New Mexico State Fairgrounds Creative Arts Building (EXPO New Mexico) NMGCA 66th ANNIVERSARY FALL GUN, SWORD & KNIFE SHOW October 3 & 4 New Mexico State Fairgrounds Manuel Lujan Building (EXPO New Mexico) For Table Reservations, Contact Our Show Host: NMGCA Show Host, P.O. Box 13687, Albuquerque, NM 87192. Ph. 505-262-1350. Email: [email protected]. For additional information visit our website at: NMGCA.NET. Set-Up (Table Holders Only) Fri 12:00 Noon - 8:00 pm, Sat. 8:00 - 9:00 am. (Unless you notify the Show Host before Saturday that you need to check in after 8:30 am Saturday, your tables may be sold to those on the waiting list.) Open to Public: Sat 9:00 am to 5:00 pm, Sun. 9:00 am to 4:00 pm. Admission-Two Day Entry (No Debit/Credit Cards): General Admission* - $10.00. NRA Members with Card - $9.00. Uniformed Law Enforcement Officers - Free. NMGCA members Free with Current Membership Card *Children under 12 - Free. Anyone under 18 must be accompanied by a guardian - I.D. required. No Alcohol, No Smoking, and No Dogs. JANUARY/FEBRUARY 2020 TABLE RATE PRICES Due to the increases in costs (i.e. insurances, State Fair (venue) related charges, etc.) it was necessary to raise table rates by $10.00 per table (as shown below) commencing January 1, 2020. -
National Rifle Association Journal Spring 2011 Volume XC Number 1
National Rifle Association Journal Spring 2011 Volume XC Number 1 NATIONAL RIFLE ASSOCIATION Contents JOURNAL 2 Charges for 2011 SPRING 2011 3 Notes from the Secretary General VOLUME XC NUMBER 1 6 Shooting Matters – Competitions Published three times a year by the 12 Notes from the Managing Director of NSC National Rifle Association 14 Shooting Matters – Range Operations Bisley, National Shooting Centre 15 Notes from the Director of Training Brookwood, Surrey GU24 0PB 18 Notes from the Firearms Liaison Officer Telephone: 01483 797777 21 Shooting Discipline Matters 0845 1307620 (local rate) 27 Regional Matters Fax: 01483 797285 30 Forthcoming Tours Range Office: 01483 797777 ext 152 31 Australia Match 2010 Clay Range Office: 01483 797666 34 Imperial Meeting Online Survey E-mail: [email protected] 40 Great Britain Team to Canada 2010 Website: http://www.nra.org.uk 46 Bisley V Club Match 47 We’re Going Green! Chairman: Robin Pizer 48 Long Range Shooting with the .22” Long Secretary General: Glynn Alger Rifle Cartridge Membership Secretary: Heather Webb 54 100 Years Ago Managing Director NSC: Jeremy Staples MRICS 55 F Class at the Imperial Financial Manager: Graham Gold FCCA Editor: Karen Robertson 57 My Bisley Camp Editorial Advisory Panel: 58 Lt Col Charles Robert Crosse CMG MVO Glynn Alger, David Cooper, Tim Elliott, 59 Scottish Rifle eamT to Australia 2010 Colin Judge, Tony de Launay, Steve McDowell, 63 The Enemy Ted Molyneux, David Pollard 64 Letters 65 Obituaries Advertising: 71 Procedure for the General Council Elections Print-Rite, 31 Parklands, Freeland, 72 Trade Members Nr Witney, Oxon OX29 8HX Tel/Fax: 01993 881662 Advertisers index Material for inclusion in the Journal should be sent to: Karen Robertson 29 A1 Hearing National RifleAssociation 18 Ayling Cars Bisley, National Shooting Centre 13 Bisley Live Brookwood, Surrey GU24 0PB 56 Bisley Pavilion Telephone: 01483 797777 ext 146 44 Centra E-mail: [email protected] 30 Norman Clark 38 Diverse Trading Company Production and distribution of the NRA Journal by 5 A Ford Print-Rite, Oxford. -
Measuring Peroxyoxalate Chemiluminescence Using a Spectrofluorophotometer No.A493
LAAN-A-RF-E003 Application Spectrophotometric Analysis News Measuring Peroxyoxalate Chemiluminescence Using a Spectrofluorophotometer No.A493 Chemiluminescence is a phenomenon where molecules n Fluorescent Dyes Used in Glow Sticks are excited by a chemical reaction and then emit light Commercially marketed glow sticks are shown in Fig. 2. energy as they return to ground state. Chemiluminescence The oxalate ester and fluorescent dye solution are placed based on using oxalate esters features high-emission in a sealed thin-walled glass container and the glass efficiency and long emission time and provides container is placed inside a polyethylene tube together illumination for long periods without any electricity. with hydrogen peroxide solution to which a catalyst is Consequently, it is used for recreational, fishing, and many added. Bending the polyethylene tube breaks the glass other applications where it is commonly called glow sticks. container, which causes both solutions to mix together The following describes the luminescent process of and emit light. peroxyoxalate chemiluminescence and gives an example Examples of the fluorescent dyes used in glow sticks are of using an RF-6000 spectrofluorophotometer to measure shown in Fig. 3. Polycyclic aromatic fluorescent dyes are the emission spectra of glow sticks. used, which emit different colors based on the wavelength of light emitted when the fluorescent dyes change from excited to ground state. n Luminescent Process of Peroxyoxalate Chemiluminescence Peroxyoxalate chemiluminescence is caused by a chemical O RO O reaction between an oxalate ester and hydrogen peroxide O C within a fluorescent dye solution. As shown in Fig. 1, the C C + H2O2 2ROH + O C oxalate ester is oxidized by hydrogen peroxide to produce O OR O ROH and 1,2-dioxetanedione. -
Biofluorescence
Things That Glow In The Dark Classroom Activities That Explore Spectra and Fluorescence Linda Shore [email protected] “Hot Topics: Research Revelations from the Biotech Revolution” Saturday, April 19, 2008 Caltech-Exploratorium Learning Lab (CELL) Workshop Special Guest: Dr. Rusty Lansford, Senior Scientist and Instructor, Caltech Contents Exploring Spectra – Using a spectrascope to examine many different kinds of common continuous, emission, and absorption spectra. Luminescence – A complete description of many different examples of luminescence in the natural and engineered world. Exploratorium Teacher Institute Page 1 © 2008 Exploratorium, all rights reserved Exploring Spectra (by Paul Doherty and Linda Shore) Using a spectrometer The project Star spectrometer can be used to look at the spectra of many different sources. It is available from Learning Technologies, for under $20. Learning Technologies, Inc., 59 Walden St., Cambridge, MA 02140 You can also build your own spectroscope. http://www.exo.net/~pauld/activities/CDspectrometer/cdspectrometer.html Incandescent light An incandescent light has a continuous spectrum with all visible colors present. There are no bright lines and no dark lines in the spectrum. This is one of the most important spectra, a blackbody spectrum emitted by a hot object. The blackbody spectrum is a function of temperature, cooler objects emit redder light, hotter objects white or even bluish light. Fluorescent light The spectrum of a fluorescent light has bright lines and a continuous spectrum. The bright lines come from mercury gas inside the tube while the continuous spectrum comes from the phosphor coating lining the interior of the tube. Exploratorium Teacher Institute Page 2 © 2008 Exploratorium, all rights reserved CLF Light There is a new kind of fluorescent called a CFL (compact fluorescent lamp). -
Small Caliber Ammo ID Vol 1
-. t, DST-1160G-514-78-VOL I " O DEFENSE INTELLIGENCE AGENCY EELECTE , J.44LL-CALIbER AMMUNITION IDENTIFICATION GUIDE Jill VOLUME 1 SMALL-ARMS CARTRIDGES UP ki 15 MM (UJ ,.-... tI., .: lAP. , UVý7J) FCl u•r~UBk'L'' 4UL.:I- DIkralUUTIG UNLIMITED "PREPARED BY US ARMY "Y,..i.,fERIEL [)EA'F!•M) ,aT AN, RLADIN"SS OMMAt,!D .'.'R'-GN SCIENCE AND TECH.NIOLOGY CENiIF~ ,. __ . .. .. ._.--. .,----..-. ... --.-... , .... R. T. Hutngo Vc111ma 197 Smell-Armsartidges Uptuf Datme(U Novernlwr 1977 ThiiS PUbliC.itiuii SUPC-(&pcsd SCC -68 i.i a I )cpartniin nE )iD fe ns~[it IlCI~g1ciic C CL .11unn C pr ,in.r, d 1,% Ii UILX11',11 S WIIALC anjild1CIIoIlog CA-tter, tJS Arwy Maicricl DevdqI[1cnt .n I~ch~~n:Cinnaid.~dapprowe b% tho )cpiucv D;ri t~ir furA. S(it'ittitil and TcdIiiical I.tehgllgeicof dthe I)cfciisc Ingclligncir Ageiilcx )ViA I\'I([ P1UBLIC: KIFLASI.: IDISTIIBltt ION (INLIMI'IIUIA) (IRce:%.c ISI.111K) -Z PREFACE This guide outlin&:s a systematic procedure fur identifying milt..rv c~rtgidgL :. e c.. rtridge designiation, country of nianufactuve. and--to a large cxtent-functionial 'bullet cyc~c kVcs'-;ncd Cor usc by persons who may not be familiar with small-arms ammunition, it pirovides L'.wsa inioniation on car-tridge types, construction, and terminology as well as more detailed identification dALa. This guide covers military cartridges in calbrs of 15 mim and below-as well as sevcra! rLllt.cd patamilitary cr target cartridges- that have been mwizufacturcd or used since 1930. Although sm if thec cartridges ini this guide arc obsolete in the country of manufacture, they are included because they were madk: in such large quantities that c . -
Following the Thread: Arms and Ammunition Tracing in Sudan and South Sudan
32 Following the Thread: Arms and Ammunition Tracing in Sudan and South Sudan By Jonah Leff and Emile LeBrun Copyright Published in Switzerland by the Small Arms Survey © Small Arms Survey, Graduate Institute of International and Development Studies, Geneva 2014 First published in May 2014 All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, without prior permission in writing of the Small Arms Survey, or as expressly permitted by law, or under terms agreed with the appropriate reprographics rights organi- zation. Enquiries concerning reproduction outside the scope of the above should be sent to the Publications Manager, Small Arms Survey, at the address below. Small Arms Survey Graduate Institute of International and Development Studies 47 Avenue Blanc, 1202 Geneva, Switzerland Series editor: Emile LeBrun Copy-edited by Tania Inowlocki Proofread by Donald Strachan ([email protected]) Cartography by Jillian Luff (www.mapgrafix.com) Typeset in Optima and Palatino by Rick Jones ([email protected]) Printed by nbmedia in Geneva, Switzerland ISBN 978-2-9700897-1-1 2 Small Arms Survey HSBA Working Paper 32 Contents List of boxes, figures, maps, and tables .......................................................................................................................... 5 List of abbreviations .................................................................................................................................................................................... -
Light Stick Chemiluminescence
High Touch High Tech® Science Experiences That Come To You Light Stick Chemiluminescence Supplies: 3 light sticks 3 clear plastic cups (9 oz.) water microwave ice Instructions: For this experiment, you will find out how temperature affects the chemical reaction that occurs in light sticks. The chemiluminescence depends on the mixing of the hydrogen peroxide with the catalyst chemical inside the light stick. Catalysts speed up and help chemical reactions! You will observe how the light sticks react when placed in three different temperatures of water. 1. Fill the first cup with ice-cold water. 2. Fill the second cup with room temperature water. 3. Fill the third cup with hot water. **Ask an adult to help you heat up the water in the microwave and pour it into the third cup.** 4. Do not break the light sticks, yet. Place one stick into each cup. 5. Allow the sticks to sit in the water for 3 minutes. This will allow the chemicals inside the light sticks to adjust to the temperature of the water. 6. Turn off the lights. 7. Bend the sticks to break the capsules inside. 8. Place the light sticks back into their designated cups. 9. Let them sit in the water, and watch the light sticks. 10. Notice how quickly or slowly the color changes. 11. Compare the brightness of each light stick. Which cup holds the brightest stick? Which light stick is the dimmest? How quickly did the light sticks glow? ScienceMadeFun.net • 800.444.4968 High Touch High Tech® Science Experiences That Come To You How long do the light sticks keep their glow? Why do you think the temperature of the water affects the light? The Science Behind It: Light produced by a chemical reaction is called chemiluminescence. -
Imagequant™ LAS 4000 User Manual
GE Healthcare ImageQuant™ LAS 4000 User Manual Table of Contents Table of Contents 1 Introduction ...................................................................................................................................................... 5 1.1 Important user information ............................................................................................................................................ 5 2 The ImageQuant LAS 4000 ............................................................................................................................. 10 2.1 The ImageQuant LAS 4000 exterior ........................................................................................................................... 10 2.2 Inside the ImageQuant LAS 4000 ................................................................................................................................ 11 2.3 Connections ........................................................................................................................................................................... 12 2.4 Parts and accessories ....................................................................................................................................................... 14 3 Exchanging accessory parts .......................................................................................................................... 17 3.1 Changing or installing a filter ........................................................................................................................................ -
Review of Chemiluminescence As an Optical Diagnostic Tool for High Pressure Unstable Rockets Tristan Latimer Fuller Purdue University
Purdue University Purdue e-Pubs Open Access Theses Theses and Dissertations January 2015 Review of Chemiluminescence as an Optical Diagnostic Tool for High Pressure Unstable Rockets Tristan Latimer Fuller Purdue University Follow this and additional works at: https://docs.lib.purdue.edu/open_access_theses Recommended Citation Fuller, Tristan Latimer, "Review of Chemiluminescence as an Optical Diagnostic Tool for High Pressure Unstable Rockets" (2015). Open Access Theses. 1176. https://docs.lib.purdue.edu/open_access_theses/1176 This document has been made available through Purdue e-Pubs, a service of the Purdue University Libraries. Please contact [email protected] for additional information. Graduate School Form 30 Updated 1/15/2015 PURDUE UNIVERSITY GRADUATE SCHOOL Thesis/Dissertation Acceptance This is to certify that the thesis/dissertation prepared By Tristan L. Fuller Entitled REVIEW OF CHEMILUMINESCENCE AS AN OPTICAL DIAGNOSTIC TOOL FOR HIGH PRESSURE UNSTABLE ROCKETS For the degree of Master of Science in Chemical Engineering Is approved by the final examining committee: William E. Anderson Chair Robert P. Lucht Stephen D. Heister Carson D. Slabaugh To the best of my knowledge and as understood by the student in the Thesis/Dissertation Agreement, Publication Delay, and Certification Disclaimer (Graduate School Form 32), this thesis/dissertation adheres to the provisions of Purdue University’s “Policy of Integrity in Research” and the use of copyright material. Approved by Major Professor(s): William E. Anderson Approved by: Weinong W. Chen 7/24/2015 Head of the Departmental Graduate Program Date REVIEW OF CHEMILUMINESCENCE AS AN OPTICAL DIAGNOSTIC TOOL IN HIGH PRESSURE UNSTABLE COMBUSTORS A Thesis Submitted to the Faculty of Purdue University by Tristan L. -
3 & Fluoromax®-P
Fluoromax-3 v. 2.0 (1 Oct 2001) FluoroMax®-3 & FluoroMax®-P Operation Manual http://www.isainc.com Rev. 2 In the USA: Jobin Yvon Inc. 3880 Park Avenue, Edison, NJ 08820 In France: Japan: (81) 3/5823.0140 Tel: 1-732-494-8660 16-18, rue du Canal China: (86) 10/6836.6542 Fax: 1-732-549-5157 91165 Longjumeau cedex Germany: (49) 89/46.23.17-0 E-Mail: [email protected] Tel: (33) 1/64.54.13.00 Italy: (39) 2/57.60.47.62 1-800-533-5946 Fax: (33) 1/69.09.93.19 U.K.: (44) 208/2048142 i Fluoromax-3 v. 2.0 (1 Oct 2001) Copyright ©2001 by Jobin Yvon Inc. All rights reserved. No part of this work may be reproduced, stored, in a retrieval system, or transmitted in any form by any means, including electronic or mechanical, photocopying and recording, without prior written permission from Jobin Yvon Inc. Requests for permission should be requested in writing. Information in this manual is subject to change without notice, and does not represent a commitment on the part of the vendor. October 2001 Part Number 81038 ii Fluoromax-3 v. 2.0 (1 Oct 2001) Table of Contents 0: Introduction ................................................................................................ 0-1 About the FluoroMax®-3 and FluoroMax®-P........................................................................................... 0-1 Chapter overview.................................................................................................................................... 0-2 Symbols used in this manual.................................................................................................................