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3 & Fluoromax®-P Fluoromax-3 v. 2.0 (1 Oct 2001) FluoroMax®-3 & FluoroMax®-P Operation Manual http://www.isainc.com Rev. 2 In the USA: Jobin Yvon Inc. 3880 Park Avenue, Edison, NJ 08820 In France: Japan: (81) 3/5823.0140 Tel: 1-732-494-8660 16-18, rue du Canal China: (86) 10/6836.6542 Fax: 1-732-549-5157 91165 Longjumeau cedex Germany: (49) 89/46.23.17-0 E-Mail: [email protected] Tel: (33) 1/64.54.13.00 Italy: (39) 2/57.60.47.62 1-800-533-5946 Fax: (33) 1/69.09.93.19 U.K.: (44) 208/2048142 i Fluoromax-3 v. 2.0 (1 Oct 2001) Copyright ©2001 by Jobin Yvon Inc. All rights reserved. No part of this work may be reproduced, stored, in a retrieval system, or transmitted in any form by any means, including electronic or mechanical, photocopying and recording, without prior written permission from Jobin Yvon Inc. Requests for permission should be requested in writing. Information in this manual is subject to change without notice, and does not represent a commitment on the part of the vendor. October 2001 Part Number 81038 ii Fluoromax-3 v. 2.0 (1 Oct 2001) Table of Contents 0: Introduction ................................................................................................ 0-1 About the FluoroMax®-3 and FluoroMax®-P........................................................................................... 0-1 Chapter overview.................................................................................................................................... 0-2 Symbols used in this manual.................................................................................................................. 0-3 1: Requirements & Installation ............................................................................ 1-1 Surface requirements ............................................................................................................................. 1-1 Environmental requirements................................................................................................................... 1-2 Electrical requirements ........................................................................................................................... 1-3 Unpacking and Installation ..................................................................................................................... 1-4 2: System Description....................................................................................... 2-1 Introduction ............................................................................................................................................. 2-1 Basic theory of operation........................................................................................................................ 2-1 Optical layout .......................................................................................................................................... 2-1 3: System Operation......................................................................................... 3-1 Introduction ............................................................................................................................................. 3-1 Controls and indicators........................................................................................................................... 3-1 Turning on the system ............................................................................................................................ 3-2 Checking system performance............................................................................................................... 3-3 4: Data Acquisition........................................................................................... 4-1 Real Time Display................................................................................................................................... 4-2 Visual Instrument Setup ......................................................................................................................... 4-3 Run Experiment...................................................................................................................................... 4-4 Constant Wavelength Analysis............................................................................................................... 4-9 Running an unknown sample ............................................................................................................... 4-10 5: Optimizing Data ........................................................................................... 5-1 Cuvette preparation ................................................................................................................................ 5-1 Sample preparation ................................................................................................................................ 5-2 Measuring the G factor ........................................................................................................................... 5-3 Improving the signal-to-noise ratio ......................................................................................................... 5-6 Correcting data ..................................................................................................................................... 5-12 6: Maintenance............................................................................................... 6-1 Introduction ............................................................................................................................................. 6-1 Lamp replacement.................................................................................................................................. 6-1 Electronics .............................................................................................................................................. 6-8 7: Components & Accessories ............................................................................. 7-1 Itemized list of FluoroMax®-3 accessories ............................................................................................. 7-2 Model 1940 Absorption/Transmission Accessory .................................................................................. 7-3 FL-1013 Liquid Nitrogen Dewar Assembly............................................................................................. 7-6 Model 1908MOD Scatter Block Assembly ............................................................................................. 7-7 Model 1908 Standard Lamp Assembly .................................................................................................. 7-7 Sample cells ........................................................................................................................................... 7-8 F-3000 Fiber Optic Mount....................................................................................................................... 7-9 Model 1938 Cut-On Filter ..................................................................................................................... 7-10 Model 1939 Cut-On Filter ..................................................................................................................... 7-10 FL-1010 Cut-On Filter Holder............................................................................................................... 7-11 FL-1011 Four-Position Thermostatted Cell Holder .............................................................................. 7-12 FL-1012 Dual-Position Thermostatted Cell Holder .............................................................................. 7-14 Model 1933 Solid Sample Holder......................................................................................................... 7-16 iii Fluoromax-3 v. 2.0 (1 Oct 2001) F-3005/6 Autotitration Injector .............................................................................................................. 7-18 Models F-3001, F-3002, and F-3003 Microscope Fiber-Optic Interfaces ............................................ 7-19 Model 1905-OFR 150-W Xenon Lamp................................................................................................. 7-20 F-3004 Sample Heater/Cooler Peltier Thermocouple Drive ................................................................ 7-21 MicroMax Microwell Plate Reader........................................................................................................ 7-22 Fl-1044 L-Format Polarizer................................................................................................................... 7-23 FL-1015 Injector Port............................................................................................................................ 7-24 F-1001/1 Temperature Bath ................................................................................................................. 7-25 Model TRIG-15/25 External Trigger Accessory ................................................................................... 7-26 FM-2005 Upgrade to FluoroMax®-P..................................................................................................... 7-27 8: Troubleshooting........................................................................................... 8-1 Using diagnostic spectra ........................................................................................................................ 8-4 Further assistance… .............................................................................................................................
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