Activities Related to Poplar and Willow Cultivation, Exploitation, And
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Phenotypic Impacts of PBAN RNA Interference in an Ant, Solenopsis Invicta, and a Moth, Helicoverpa Zea ⇑ ⇑ Man-Yeon Choi A, , Robert K
Journal of Insect Physiology 58 (2012) 1159–1165 Contents lists available at SciVerse ScienceDirect Journal of Insect Physiology journal homepage: www.elsevier.com/locate/jinsphys Phenotypic impacts of PBAN RNA interference in an ant, Solenopsis invicta, and a moth, Helicoverpa zea ⇑ ⇑ Man-Yeon Choi a, , Robert K. Vander Meer a, , Monique Coy b, Michael E. Scharf b,c a U. S. Department of Agriculture, Agricultural Research Service, Center of Medical, Agricultural and Veterinary Entomology (CMAVE), 1600 SW, 23rd Drive, Gainesville, FL 32608, USA b Department of Entomology and Nematology, University of Florida, Gainesville, FL 32608, USA c Department of Entomology, Purdue University, West Lafayette, IN 47907, USA article info abstract Article history: Insect neuropeptide hormones represent more than 90% of all insect hormones. The PBAN/pyrokinin fam- Received 30 January 2012 ily is a major group of insect neuropeptides, and they are expected to be found from all insect groups. Received in revised form 1 June 2012 These species-specific neuropeptides have been shown to have a variety of functions from embryo to Accepted 5 June 2012 adult. PBAN is well understood in moth species relative to sex pheromone biosynthesis, but other poten- Available online 13 June 2012 tial functions are yet to be determined. Recently, we focused on defining the PBAN gene and peptides in fire ants in preparation for an investigation of their function(s). RNA interference (RNAi) technology is a Keywords: convenient tool to investigate unknown physiological functions in insects, and it is now an emerging PBAN method for development of novel biologically-based control agents as alternatives to insecticides. -
Molecular Basis of Pheromonogenesis Regulation in Moths
Chapter 8 Molecular Basis of Pheromonogenesis Regulation in Moths J. Joe Hull and Adrien Fónagy Abstract Sexual communication among the vast majority of moths typically involves the synthesis and release of species-specifc, multicomponent blends of sex pheromones (types of insect semiochemicals) by females. These compounds are then interpreted by conspecifc males as olfactory cues regarding female reproduc- tive readiness and assist in pinpointing the spatial location of emitting females. Studies by multiple groups using different model systems have shown that most sex pheromones are synthesized de novo from acetyl-CoA by functionally specialized cells that comprise the pheromone gland. Although signifcant progress was made in identifying pheromone components and elucidating their biosynthetic pathways, it wasn’t until the advent of modern molecular approaches and the increased avail- ability of genetic resources that a more complete understanding of the molecular basis underlying pheromonogenesis was developed. Pheromonogenesis is regulated by a neuropeptide termed Pheromone Biosynthesis Activating Neuropeptide (PBAN) that acts on a G protein-coupled receptor expressed at the surface of phero- mone gland cells. Activation of the PBAN receptor (PBANR) triggers a signal trans- duction cascade that utilizes an infux of extracellular Ca2+ to drive the concerted action of multiple enzymatic steps (i.e. chain-shortening, desaturation, and fatty acyl reduction) that generate the multicomponent pheromone blends specifc to each species. In this chapter, we provide a brief overview of moth sex pheromones before expanding on the molecular mechanisms regulating pheromonogenesis, and con- clude by highlighting recent developments in the literature that disrupt/exploit this critical pathway. J. J. Hull (*) USDA-ARS, US Arid Land Agricultural Research Center, Maricopa, AZ, USA e-mail: [email protected] A. -
Elpenor 2010-2015
Projet ELPENOR MACROHETEROCERES DU CANTON DE GENEVE : POINTAGE DES ESPECES PRESENTES Résultats des prospections 2010-2015 Pierre BAUMGART & Maxime PASTORE « Voilà donc les macrohétérocéristes ! Je les imaginais introvertis, le teint blafard, disséquant, cataloguant, épinglant. Ils sont là, enjoués, passionnés, émerveillés par les trésors enfouis des nuits genevoises ! » Blaise Hofman, « La clé des champs » SOMMAIRE • ELPENOR ? 2 • INTRODUCTION 3 • PROTOCOLE DE CHASSE 4 • FICHE D’OBSERVATIONS 4 • MATÉRIEL DE TERRAIN 5 • SITES PROSPECTÉS 7 - 11 • ESPÈCES OBSERVÉES 2010 – 2015 13 (+ 18 p. hors-texte) • ESPECES OBSERVEES CHAQUE ANNEE 13’ • ECHANTILLONNAGE D’ESPECES 14 • CHRONOLOGIE DES OBSERVATIONS REMARQUABLES 15 – 17 • ESPECES A RECHERCHER 18-20 • AUTRES VISITEURS… 21 • PUBLICATIONS 22 (+ 4 p. hors-texte) • ESPECES AJOUTEES A LA LISTE 23 • RARETÉS 24 • DISCUSSION 25 - 26 • PERSPECTIVES 27 • CHOIX DE CROQUIS DE TERRAIN 29 - 31 • COUPURES DE PRESSE 33 - 35 • ALBUM DE FAMILLE 36 • REMERCIEMENTS 37 • BIBLIOGRAPHIE & RESSOURCES INTERNET 38 – 39 1 ELPENOR ? Marin et compagnon d'Ulysse à son retour de la guerre de Troie, Elpenor (en grec Ἐλπήνορος , « homme de l'espoir ») est de ceux qui, sur l'île d'Aenea, furent victimes de la magicienne Circé et transformés en pourceaux jusqu'à ce qu'Ulysse, qui avait été préservé des enchantements de la magicienne grâce à une herbe offerte par le dieu Hermès, la contraigne à redonner à ses compagnons leur forme humaine. Lors de la fête qui s’ensuivit, Elpenor, pris de boisson, s'endormit sur la terrasse de la demeure de Circé, et, réveillé en sursaut, se tua en tombant du toit. Lorsqu'il descendit aux Enfers pour consulter le devin Tirésias, Ulysse croisa l’ombre de son défunt compagnon, à laquelle il promit une sépulture honorable. -
Effect of Micromelalopha Sieversi (Staudinger) Oviposition
Article Effect of Micromelalopha sieversi (Staudinger) Oviposition Behavior on the Transcriptome of Two Populus Section Aigeiros Clones Li Guo 1,2, Sufang Zhang 1, Fu Liu 1 , Xiangbo Kong 1 and Zhen Zhang 1,* 1 Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China; [email protected] (L.G.); [email protected] (S.Z.); [email protected] (F.L.); [email protected] (X.K.) 2 School of Biological Science and Engineering, Xingtai University, Xingtai 054001, China * Correspondence: [email protected]; Tel.: +86-136-7102-2209 Received: 3 September 2020; Accepted: 16 September 2020; Published: 22 September 2020 Abstract: Research Highlights: The molecular mechanisms underlying woody plant resistance upon oviposition by herbivores remain unclear, as studies have focused on herbaceous plants. The effect of oviposition on gene expression in neighboring plants has also not been reported. Elucidating these molecular responses can help cultivate insect-resistant trees. Background and Objectives: Oviposition by herbivorous insects acts as an early warning signal, inducing plant resistance responses. Here, we employed poplar as a model woody plant to elucidate gene expression and the molecular mechanisms underlying plant resistance after oviposition by Micromelalopha sieversi (Staudinger) (Lepidoptera: Notodontidae). Materials and Methods: The differences in gene expression of two Populus section Aigeiros clones (‘108’ (Populus euramericana ‘Guariento’) and ‘111’ × (Populus euramericana ‘Bellotto’)) were analyzed via high-throughput sequencing of oviposited, × neighboring, and control plants. Results: We obtained 304,526,107 reads, with an average length of 300 bp and a total size of 40.77 Gb. Differentially expressed genes (DEGs) in gene ontology terms of biological process, cellular component, and molecular function were mainly enriched in the “cell part”, “catalytic”, and “metabolic process” functions. -
The Isolation, Genetic Characterisation And
The isolation, genetic characterisation and biological activity of a South African Phthorimaea operculella granulovirus (PhopGV-SA) for the control of the Potato Tuber Moth, Phthorimaea operculella (Zeller) A thesis submitted in fulfilment of the requirements for the degree of MASTER OF SCIENCE At RHODES UNIVERSITY By MICHAEL DAVID JUKES February 2015 i Abstract The potato tuber moth, Phthorimaea operculella (Zeller), is a major pest of potato crops worldwide causing significant damage to both field and stored tubers. The current control method in South Africa involves chemical insecticides, however, there is growing concern on the health and environmental risks of their use. The development of novel biopesticide based control methods may offer a potential solution for the future of insecticides. In this study a baculovirus was successfully isolated from a laboratory population of P. operculella. Transmission electron micrographs revealed granulovirus-like particles. DNA was extracted from recovered occlusion bodies and used for the PCR amplification of the lef-8, lef- 9, granulin and egt genes. Sequence data was obtained and submitted to BLAST identifying the virus as a South African isolate of Phthorimaea operculella granulovirus (PhopGV-SA). Phylogenetic analysis of the lef-8, lef-9 and granulin amino acid sequences grouped the South African isolate with PhopGV-1346. Comparison of egt sequence data identified PhopGV-SA as a type II egt gene. A phylogenetic analysis of egt amino acid sequences grouped all type II genes, including PhopGV-SA, into a separate clade from types I, III, IV and V. These findings suggest that type II may represent the prototype structure for this gene with the evolution of types I, III and IV a result of large internal deletion events and subsequent divergence. -
Biodiversity, Evolution and Ecological Specialization of Baculoviruses: A
Biodiversity, Evolution and Ecological Specialization of Baculoviruses: A Treasure Trove for Future Applied Research Julien Thézé, Carlos Lopez-Vaamonde, Jenny Cory, Elisabeth Herniou To cite this version: Julien Thézé, Carlos Lopez-Vaamonde, Jenny Cory, Elisabeth Herniou. Biodiversity, Evolution and Ecological Specialization of Baculoviruses: A Treasure Trove for Future Applied Research. Viruses, MDPI, 2018, 10 (7), pp.366. 10.3390/v10070366. hal-02140538 HAL Id: hal-02140538 https://hal.archives-ouvertes.fr/hal-02140538 Submitted on 26 May 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution| 4.0 International License viruses Article Biodiversity, Evolution and Ecological Specialization of Baculoviruses: A Treasure Trove for Future Applied Research Julien Thézé 1,2, Carlos Lopez-Vaamonde 1,3 ID , Jenny S. Cory 4 and Elisabeth A. Herniou 1,* ID 1 Institut de Recherche sur la Biologie de l’Insecte, UMR 7261, CNRS—Université de Tours, 37200 Tours, France; [email protected] (J.T.); [email protected] -
Viruses 2015, 7, 306-319; Doi:10.3390/V7010306 OPEN ACCESS
Viruses 2015, 7, 306-319; doi:10.3390/v7010306 OPEN ACCESS viruses ISSN 1999-4915 www.mdpi.com/journal/viruses Review History and Current Status of Development and Use of Viral Insecticides in China Xiulian Sun Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China; E-Mail: [email protected]; Tel.: +86-27-8719-8641; Fax: +86-27-8719-8072 Academic Editors: John Burand and Madoka Nakai Received: 1 December 2014 / Accepted: 14 January 2015 / Published: 20 January 2015 Abstract: The use of insect viruses as biological control agents started in the early 1960s in China. To date, more than 32 viruses have been used to control insect pests in agriculture, forestry, pastures, and domestic gardens in China. In 2014, 57 products from 11 viruses were authorized as commercial viral insecticides by the Ministry of Agriculture of China. Approximately 1600 tons of viral insecticidal formulations have been produced annually in recent years, accounting for about 0.2% of the total insecticide output of China. The development and use of Helicoverpa armigera nucleopolyhedrovirus, Mamestra brassicae nucleopolyhedrovirus, Spodoptera litura nucleopolyhedrovirus, and Periplaneta fuliginosa densovirus are discussed as case studies. Additionally, some baculoviruses have been genetically modified to improve their killing rate, infectivity, and ultraviolet resistance. In this context, the biosafety assessment of a genetically modified Helicoverpa armigera nucleopolyhedrovirus is discussed. Keywords: viral insecticides; commercialization; genetic modification 1. Introduction Research on insect viruses in China started with the Bombyx mori nucleopolyhedrovirus in the mid-1950s [1] and, to date, more than 200 insect virus isolates have been recorded in China. -
Diplomová Práce
Západočeská univerzita v Plzni Pedagogická fakulta Centrum biologie, geověd a envigogiky Revize sbírky motýlů J. Fraje s důrazem na motýly Plzeňského kraje DIPLOMOVÁ PRÁCE Autor práce: Vedoucí práce: Bc. Jan Walter Mgr. Ivana Hradská Plzeň 2020 PROHLÁŠENÍ Prohlašuji, že jsem diplomovou práci na téma "Revize lepidopterologické sbírky J. Fraje s důrazem na motýly Plzeňského kraje" vypravoval samostatně a s pomocí odborné literatury a dalších informačních zdrojů, které jsou uvedeny v seznamu literatury. V Plzni dne …………………… ………………………………… Jan Walter PODĚKOVÁNÍ Na prvém místě bych chtěl poděkovat Ivaně Hradské za ochotu při vedení mé diplomové práce, za kritické připomínky, za veškerý poskytnutý materiál, morální podporu a svůj čas, který mi věnovala a věnuje. Také kolegům Vlastimilu Cihlářovi a Pavlovi Vrbovi za pomoc při určení některých druhů, Stanislavu Vodičkovi za pomoc s tvorbou databáze, Zbyňku Kejvalovi za poskytnutí manuskriptů z inventarizačních průzkumů a v neposlední řadě rodině a přátelům za podporu. ZADÁNÍ PRÁCE ABSTRAKT Sbírka Jaroslava Fraje patří k rozsáhlejším položkám sbírkového fondu zoologického oddělení Západočeského muzea v Plzni. Z deponovaných sbírkových krabic pochází údaje o 653 druzích motýlů zahrnutých v 15 čeledí. Mezi nejcennější výsledky patří záznamy o chráněných nebo ohrožených druzích s výskytem v Plzeňském kraji – Pharmacis fusconebulosa, Phymatopus hecta, Hepialus humuli, Carcharodus alceae, Hesperia comma, Leptidea sinapis, Satyrium pruni, Callophrys rubi, Boloria selene, Boloria euphrosyne, Melitaea -
Working Party on Poplar and Willow Insects and Other Animal Pests
WORKING PARTY ON POPLAR AND WILLOW INSECTS AND OTHER ANIMAL PESTS 169 170 PRESENT SITUATION OF THE POPULATION OF N. OLIGOSPILUS FOERSTER (=N. DESANTISI SMITH) (HYM.: TENTHREDINIDAE) IN THE TAFI VALLEY, TUCUMAN, ARGENTINA: FUTURE CONSIDERATIONS Mariela Alderete1, Gerardo Liljesthröm Nematus oligospilus Foerster (= N. desantisi Smith), a Holartic species whose larvae feed on leaves of Salix spp., was recorded in Argentina and Chile in the 1980´s. In the delta of the Paraná river (DP) and in the Tafí valley (VT) in Argentina, the sawfly larval populations attained high densities and severe defoliations were observed: in 1991-92 and 1993-94 in DP, and in 1990-91 and 1994-95 in VT. In VT the sawfly larvae have remained at low density since then and trials excluding natural enemies showed that larval survivorship was significantly higher than in the controls. Further, an intensive sampling over five consecutive years allowed us to perform a key-factor analysis, and larval mortality, possibly due to predators (polyphagous Divrachys cavus was the only parasitoid recorded from less than 1% host larvae), was density-dependent and supposed to be capable of regulating the sawfly population. The DP and VT regions have different ecological conditions: while DP has broad and continuous willow plantations and a humid-temperate climate, VT is an elevated valley bordered by mountains with a sub-humid cold climate (rains are concentrated in spring and summer) with small and rather isolated willow forests. Apart from these differences, both regions show very low parasitoidism, outbreaks shortly after being recorded in the area, and no significant differences between outbreak and no-outbreak years with respect to mean and mean maximum temperatures as well as in accumulated rainfall. -
View the PDF File of the Tachinid Times, Issue 12
The Tachinid Times ISSUE 12 February 1999 Jim O’Hara, editor Agriculture & Agri-Food Canada, Biological Resources Program Eastern Cereal and Oilseed Research Centre C.E.F., Ottawa, Ontario, Canada, K1A 0C6 Correspondence: [email protected] The Tachinid Times began in 1988 when personal Evolution of Egg Structure in Tachinidae (by S.P. computers were gaining in popularity, yet before the Gaponov) advent of e-mail and the World Wide Web. A newsletter Using a scanning electron microscope I investigated distributed through the mail seemed like a useful the egg structure of 114 species of Tachinidae. The endeavour to foster greater awareness about the work of research was focused on the peculiarities of the egg others among researchers interested in the Tachinidae. surface and the structure of the aeropylar area. Data on Now, eleven years later, despite the speed and the method of egg-laying, the structure of the female convenience of e-mail and other advanced modes of reproductive system and the host range were also taken communication, this newsletter still seems to hold a place into consideration. Since any kind of adaptation is a in the distribution of news about the Tachinidae. If there result of evolution and every stage of ontogenesis, is sufficient interest - and submissions - over the course including the egg stage, is adapted to some specific of the next year, then another issue will appear in environmental conditions, each stage of ontogenesis February of the new millennium. As always, please send evolved more or less independently. The development of me your news for inclusion in the newsletter before the provisionary devices (coenogenetic adaptations) and their end of next January. -
Proceedings of the „Research Conservation, Management” Conference (Aggtelek) 1–5 May 1966
University of Debrecen JOURNAL OF AGRICULTURAL SCIENCES 2015/66 ACTA AGRARIA DEBRECENIENSIS 7th International Plant Protection Symposium at University of Debrecen 21-22 October 2015 Debrecen Editor: Dr. György J. Kövics Lectors: Dr. András Bozsik (entomology, biological pest management) Dr. Antal Nagy (entomology, ecology) Arnold Szilágyi (weed biology, weed management) Dr. István Szarukán (entomology) Dr. Gábor Tarcali (integrated pest management, plant pathology) Dr. György J. Kövics (plant pathology) Dr. László Radócz (integrated pest management, weed management) HU-ISSN 1587-1282 Contents László Radócz1 – Gábor Tarcali1 – Tünde Szabó-Asbolt1 – György János Kövics1 – László Kiss2 (1University of Debrecen, Institute of Plant Protection, Debrecen, 2Hajdú-Bihar County Organization of Hungarian Chamber of Professionals and Doctors of Plant Protection, Debrecen, Hungary): Irén Dobos horticulturalist-teacher, plant protection engineer expert awarded by „Antal Gulyás medallion for crop protection” in 2015 (laudation) 8 György János Kövics (University of Debrecen, Institute of Plant Protection, Debrecen, Hungary): Let’s talk about ’One fungus – One name’. Changes in the fungal taxonomy 11 Mahendra Rai1,2 and Gabriela Kratosova2 (1Nanobiotechnology Lab., Department of Biotechnology, SGB Amravati University, Amravati-444 602, Maharashtra state, India; 2Nanotechnology Centre, VSB - Technical University of Ostrava, Ostrava – Poruba, Czech Republic): Management of phytopathogens by application of green nanobiotechnology: Emerging trends and challenges -
Spodoptera Frugiperda Gloria Patricia Barrera1†, Mariano Nicolás Belaich2*†, Manuel Alfonso Patarroyo3,4, Laura Fernanda Villamizar1 and Pablo Daniel Ghiringhelli2
Barrera et al. BMC Genomics (2015) 16:1008 DOI 10.1186/s12864-015-2218-5 RESEARCH ARTICLE Open Access Evidence of recent interspecies horizontal gene transfer regarding nucleopolyhedrovirus infection of Spodoptera frugiperda Gloria Patricia Barrera1†, Mariano Nicolás Belaich2*†, Manuel Alfonso Patarroyo3,4, Laura Fernanda Villamizar1 and Pablo Daniel Ghiringhelli2 Abstract Background: Baculoviruses are insect-associated viruses carrying large, circular double-stranded-DNA genomes with significant biotechnological applications such as biological pest control, recombinant protein production, gene delivery in mammals and as a model of DNA genome evolution. These pathogens infect insects from the orders Lepidoptera, Hymenoptera and Diptera, and have high species diversity which is expressed in their diverse biological properties including morphology, virulence or pathogenicity. Spodoptera frugiperda (Lepidoptera: Noctuidae), the fall armyworm, represents a significant pest for agriculture in America; it is a host for baculoviruses such as the Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) (Colombia strain, genotype A) having been classified as a Group II alphabaculovirus making it a very attractive target for bioinsecticidal use. Results: Genome analysis by pyrosequencing revealed that SfMNPV ColA has 145 ORFs, 2 of which were not present in the other sequenced genotypes of the virus (SfMNPV-NicB, SfMNPV-NicG, SfMNPV-19 and SfMNPV-3AP2). An in-depth bioinformatics study showed that ORF023 and ORF024 were acquired by a recent homologous recombination process between Spodoptera frugiperda and Spodoptera litura (the Oriental leafworm moth) nucleopolyhedroviruses. Auxiliary genes are numerous in the affected locus which has a homologous region (hr3), a repetitive sequence associated with genome replication which became lost in SfColA along with 1 ORF.