DOCSLIB.ORG

Use of Next Generation Sequencing to Improve Identification and Quantification of Baculovirus Diversity

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Use of Next Generation Sequencing to Improve Identification and Quantification of Baculovirus Diversity Use of Next Generation Sequencing to Improve Identification and Quantification of Baculovirus Diversity Vom Fachbereich Biologie der Technischen Universität Darmstadt zur Erlangung des akademischen Grades eines Doctor rerum naturalium (Dr. rer. nat) Dissertation von Dipl. Biol. (Uni) Gianpiero Gueli Alletti aus Mannheim 1. Referent: Prof. Dr. Johannes A. Jehle 2. Referent: Prof. Dr. Gerhard Thiel Tag der Einreichung: 24.09.2018 Tag der mündlichen Prüfung: 07.12.2018 Darmstadt 2018D 17 Gueli Alletti, Gianpiero: Use of Next Generation Sequencing to Improve Identification and Quantification of Baculovirus Diversity Darmstadt, Technische Universität Darmstadt, Jahr der Veröffentlichung der Dissertation auf TUprints: 2018 URN: urn:nbn:de:tuda-tuprints-82879 Tag der mündlichen Prüfung: 07.12.2018 Veröffentlicht unter CC-BY-NC-ND 4.0 International https://creativecommons.org/licenses/ Für meine Frau, Inese Vieško. CONTENT CHAPTER I: GENERAL INTRODUCTION ............................................................................. 1 BACULOVIRUSES BASED INSECTICIDES: A BRIEF RETROSPECTIVE ........................................................................ 1 BACULOVIRUSES: MORPHOLOGY, INFECTION CYCLE AND TAXONOMY ................................................................ 1 BACULOVIRUS TAXONOMY AND MOLECULAR PHYLOGENY .................................................................................. 6 “CUTWORMS” AND BACULOVIRUSES INFECTING AGROTIS SPP.............................................................................. 9 THE “CODLING MOTH” AND CYDIA POMONELLA GRANULOVIRUS ...................................................................... 12 NEXT GENERATION SEQUENCING (NGS) IN BACULOVIRUS VIROLOGY AND AIMS OF THE THESIS ....................... 16 CHAPTER II: THE GENOME SEQUENCE OF AGROTIS SEGETUM GRANULOVIRUS, ISOLATE AGSEGV-DA, REVEALS A NEW BETABACULOVIRUS SPECIES OF A SLOW KILLING GRANULOVIRUS .............................................................................................................. 18 ABSTRACT.......................................................................................................................................................... 18 INTRODUCTION .................................................................................................................................................. 18 MATERIALS AND METHODS ............................................................................................................................... 20 RESULTS AND DISCUSSION ................................................................................................................................. 22 CONCLUSION ...................................................................................................................................................... 33 CHAPTER III: AGROTIS SEGETUM NUCLEOPOLYHEDROVIRUS BUT NOT AGROTIS SEGETUM GRANULOVIRUS REPLICATE IN AIE1611T CELL LINE OF AGROTIS IPSILON ..... 41 ABSTRACT.......................................................................................................................................................... 41 INTRODUCTION .................................................................................................................................................. 41 MATERIAL AND METHODS ................................................................................................................................. 42 RESULTS ............................................................................................................................................................ 46 DISCUSSION ....................................................................................................................................................... 49 CHAPTER IV: AGROTIS SEGETUM NUCLEOPOLYHEDROVIRUS B SHOWS HIGH GENOME STABILITY DURING SERIAL IN VITRO PASSAGES IN AIE1611T CELLS ................................ 51 ABSTRACT.......................................................................................................................................................... 51 INTRODUCTION .................................................................................................................................................. 51 MATERIAL AND METHODS ................................................................................................................................. 52 RESULTS ............................................................................................................................................................ 55 DISCUSSION ....................................................................................................................................................... 60 CHAPTER V: SOLEXA ILLUMINA SEQUENCING OF BAGSENPV-B REVEALS A DELETION MUTANT BACMID OF AGROTIS SEGETUM NUCLEOPOLYHEDROVIRUS B ......................... 64 ABSTRACT.......................................................................................................................................................... 64 INTRODUCTION .................................................................................................................................................. 64 MATERIAL AND METHODS ................................................................................................................................. 66 RESULTS ............................................................................................................................................................ 68 DISCUSSION ....................................................................................................................................................... 72 CHAPTER VI: NEXT GENERATION SEQUENCING TO IDENTIFY AND QUANTIFY THE GENETIC COMPOSITION OF RESISTANCE-BREAKING COMMERCIAL ISOLATES OF CYDIA POMONELLA GRANULOVIRUS ........................................................................................... 74 ABSTRACT.......................................................................................................................................................... 74 INTRODUCTION .................................................................................................................................................. 74 MATERIALS AND METHODS ............................................................................................................................... 76 RESULTS ............................................................................................................................................................ 78 DISCUSSION ....................................................................................................................................................... 88 CHAPTER VII: GENERAL DISCUSSION .............................................................................. 91 REFERNCES .................................................................................................................... 97 SUMMARY ...................................................................................................................... 110 ZUSAMMENFASSUNG ..................................................................................................... 112 APPENDIX ...................................................................................................................... 114 EIGENANTEIL DER ARBEIT (OWN WORK) ...................................................................... 120 DANKSAGUNG ................................................................................................................ 121 RESUME: GIANPIERO GUELI ALLETTI ........................................................................... 122 EHRENWÖRTLICHE ERKLÄRUNG ..................................................................................125 LIST OF ABBREVIATIONS % per cent ml mililiter × g multiple of g mM milimolar °C degree Celsius MNPV multiple nucleopolyhedrovirus µg microgram n number of tested individuals µl microliter N number of independent replicates µm micrometer n.d. not determined aa amino acid NGS Next Generation Sequencing bp base pair nm nanometer Bt Bacillus thuringiensis NPV nucleopolyhedrovirus BV budded virus nt nucleotide Organisation for Economic Co-operation and cm centimeter OECD Development ddH2O bidistilled water OB occlusion body dpi days post infection ODV occlusion derived virion DNA 2'deoxyribonucleic acid ORF open reading frame dsDNA double stranded DNA p.i. post infection e. g. for example PCR polymerase chain reaction et al. and others pH measure of acidity of basicityof aqueous solutions etc et cetera PM peritrophic membrane EU European Union RFLP restriction fragment length polymorphism g gram RNA ribonucleic acid GV granulovirus sec seconds h hour SNPV single nucleopolyhedrovirus International Commitee on ICTV spp. species pluralis; multiple species Taxonomy of Viruses JKI Julius Kühn Institute TAE tris base, acetic acid and EDTA buffer solution kb kilobase pair TBE tris base, boric acid and EDTA buffer solution LD50 median lethal dose UV ultraviolet light m meter V Volt mg miligram v/v volume in volume min minute w/v weight in volume Chapter I: General Introduction Baculoviruses based insecticides: A brief retrospective “Of all viruses known to mankind, baculoviruses are the most beneficial from an anthropocentric [sic!] viewpoint.” (Miller, 1997). Lois Kathryn Miller, always reminds us with her statement that an insect disease can
Load more

Recommended publications
  • Phenotypic Impacts of PBAN RNA Interference in an Ant, Solenopsis Invicta, and a Moth, Helicoverpa Zea ⇑ ⇑ Man-Yeon Choi A, , Robert K
    Journal of Insect Physiology 58 (2012) 1159–1165 Contents lists available at SciVerse ScienceDirect Journal of Insect Physiology journal homepage: www.elsevier.com/locate/jinsphys Phenotypic impacts of PBAN RNA interference in an ant, Solenopsis invicta, and a moth, Helicoverpa zea ⇑ ⇑ Man-Yeon Choi a, , Robert K. Vander Meer a, , Monique Coy b, Michael E. Scharf b,c a U. S. Department of Agriculture, Agricultural Research Service, Center of Medical, Agricultural and Veterinary Entomology (CMAVE), 1600 SW, 23rd Drive, Gainesville, FL 32608, USA b Department of Entomology and Nematology, University of Florida, Gainesville, FL 32608, USA c Department of Entomology, Purdue University, West Lafayette, IN 47907, USA article info abstract Article history: Insect neuropeptide hormones represent more than 90% of all insect hormones. The PBAN/pyrokinin fam- Received 30 January 2012 ily is a major group of insect neuropeptides, and they are expected to be found from all insect groups. Received in revised form 1 June 2012 These species-specific neuropeptides have been shown to have a variety of functions from embryo to Accepted 5 June 2012 adult. PBAN is well understood in moth species relative to sex pheromone biosynthesis, but other poten- Available online 13 June 2012 tial functions are yet to be determined. Recently, we focused on defining the PBAN gene and peptides in fire ants in preparation for an investigation of their function(s). RNA interference (RNAi) technology is a Keywords: convenient tool to investigate unknown physiological functions in insects, and it is now an emerging PBAN method for development of novel biologically-based control agents as alternatives to insecticides.
    [Show full text]
  • Molecular Basis of Pheromonogenesis Regulation in Moths
    Chapter 8 Molecular Basis of Pheromonogenesis Regulation in Moths J. Joe Hull and Adrien Fónagy Abstract Sexual communication among the vast majority of moths typically involves the synthesis and release of species-specifc, multicomponent blends of sex pheromones (types of insect semiochemicals) by females. These compounds are then interpreted by conspecifc males as olfactory cues regarding female reproduc- tive readiness and assist in pinpointing the spatial location of emitting females. Studies by multiple groups using different model systems have shown that most sex pheromones are synthesized de novo from acetyl-CoA by functionally specialized cells that comprise the pheromone gland. Although signifcant progress was made in identifying pheromone components and elucidating their biosynthetic pathways, it wasn’t until the advent of modern molecular approaches and the increased avail- ability of genetic resources that a more complete understanding of the molecular basis underlying pheromonogenesis was developed. Pheromonogenesis is regulated by a neuropeptide termed Pheromone Biosynthesis Activating Neuropeptide (PBAN) that acts on a G protein-coupled receptor expressed at the surface of phero- mone gland cells. Activation of the PBAN receptor (PBANR) triggers a signal trans- duction cascade that utilizes an infux of extracellular Ca2+ to drive the concerted action of multiple enzymatic steps (i.e. chain-shortening, desaturation, and fatty acyl reduction) that generate the multicomponent pheromone blends specifc to each species. In this chapter, we provide a brief overview of moth sex pheromones before expanding on the molecular mechanisms regulating pheromonogenesis, and con- clude by highlighting recent developments in the literature that disrupt/exploit this critical pathway. J. J. Hull (*) USDA-ARS, US Arid Land Agricultural Research Center, Maricopa, AZ, USA e-mail: [email protected] A.
    [Show full text]
  • Protein Composition of the Occlusion Bodies of Epinotia Aporema Granulovirus
    bioRxiv preprint doi: https://doi.org/10.1101/465021; this version posted November 7, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Protein composition of the occlusion bodies of Epinotia aporema 2 granulovirus 3 4 Tomás Masson, María Laura Fabre, María Leticia Ferrelli, Matías Luis Pidre, Víctor 5 Romanowski. 6 1 Instituto de Biotecnología y Biología Molecular (IBBM, UNLP-CONICET), Facultad de 7 Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina. 8 9 Abstract 10 Within family Baculoviridae, members of the Betabaculovirus genus are employed as 11 biocontrol agents against lepidopteran pests, either alone or in combination with 12 selected members of the Alphabaculovirus genus. Epinotia aporema granulovirus 13 (EpapGV) is a fast killing betabaculovirus that infects the bean shoot borer (E. 14 aporema) and is a promising biopesticide. Because occlusion bodies (OBs) play a key 15 role in baculovirus horizontal transmission, we investigated the composition of 16 EpapGV OBs. Using mass spectrometry-based proteomics we could identify 56 proteins 17 that are included in the OBs during the final stages of larval infection. Our data 18 provides experimental validation of several annotated hypothetical coding sequences. 19 Proteogenomic mapping against genomic sequence detected a previously unannotated 20 ac110-like core gene and a putative translation fusion product of ORFs epap48 and 21 epap49. Comparative studies of the proteomes available for the family Baculoviridae 22 highlight the conservation of core gene products as parts of the occluded virion.
    [Show full text]
  • Elpenor 2010-2015
    Projet ELPENOR MACROHETEROCERES DU CANTON DE GENEVE : POINTAGE DES ESPECES PRESENTES Résultats des prospections 2010-2015 Pierre BAUMGART & Maxime PASTORE « Voilà donc les macrohétérocéristes ! Je les imaginais introvertis, le teint blafard, disséquant, cataloguant, épinglant. Ils sont là, enjoués, passionnés, émerveillés par les trésors enfouis des nuits genevoises ! » Blaise Hofman, « La clé des champs » SOMMAIRE • ELPENOR ? 2 • INTRODUCTION 3 • PROTOCOLE DE CHASSE 4 • FICHE D’OBSERVATIONS 4 • MATÉRIEL DE TERRAIN 5 • SITES PROSPECTÉS 7 - 11 • ESPÈCES OBSERVÉES 2010 – 2015 13 (+ 18 p. hors-texte) • ESPECES OBSERVEES CHAQUE ANNEE 13’ • ECHANTILLONNAGE D’ESPECES 14 • CHRONOLOGIE DES OBSERVATIONS REMARQUABLES 15 – 17 • ESPECES A RECHERCHER 18-20 • AUTRES VISITEURS… 21 • PUBLICATIONS 22 (+ 4 p. hors-texte) • ESPECES AJOUTEES A LA LISTE 23 • RARETÉS 24 • DISCUSSION 25 - 26 • PERSPECTIVES 27 • CHOIX DE CROQUIS DE TERRAIN 29 - 31 • COUPURES DE PRESSE 33 - 35 • ALBUM DE FAMILLE 36 • REMERCIEMENTS 37 • BIBLIOGRAPHIE & RESSOURCES INTERNET 38 – 39 1 ELPENOR ? Marin et compagnon d'Ulysse à son retour de la guerre de Troie, Elpenor (en grec Ἐλπήνορος , « homme de l'espoir ») est de ceux qui, sur l'île d'Aenea, furent victimes de la magicienne Circé et transformés en pourceaux jusqu'à ce qu'Ulysse, qui avait été préservé des enchantements de la magicienne grâce à une herbe offerte par le dieu Hermès, la contraigne à redonner à ses compagnons leur forme humaine. Lors de la fête qui s’ensuivit, Elpenor, pris de boisson, s'endormit sur la terrasse de la demeure de Circé, et, réveillé en sursaut, se tua en tombant du toit. Lorsqu'il descendit aux Enfers pour consulter le devin Tirésias, Ulysse croisa l’ombre de son défunt compagnon, à laquelle il promit une sépulture honorable.
    [Show full text]
  • Effect of Micromelalopha Sieversi (Staudinger) Oviposition
    Article Effect of Micromelalopha sieversi (Staudinger) Oviposition Behavior on the Transcriptome of Two Populus Section Aigeiros Clones Li Guo 1,2, Sufang Zhang 1, Fu Liu 1 , Xiangbo Kong 1 and Zhen Zhang 1,* 1 Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China; [email protected] (L.G.); [email protected] (S.Z.); [email protected] (F.L.); [email protected] (X.K.) 2 School of Biological Science and Engineering, Xingtai University, Xingtai 054001, China * Correspondence: [email protected]; Tel.: +86-136-7102-2209 Received: 3 September 2020; Accepted: 16 September 2020; Published: 22 September 2020 Abstract: Research Highlights: The molecular mechanisms underlying woody plant resistance upon oviposition by herbivores remain unclear, as studies have focused on herbaceous plants. The effect of oviposition on gene expression in neighboring plants has also not been reported. Elucidating these molecular responses can help cultivate insect-resistant trees. Background and Objectives: Oviposition by herbivorous insects acts as an early warning signal, inducing plant resistance responses. Here, we employed poplar as a model woody plant to elucidate gene expression and the molecular mechanisms underlying plant resistance after oviposition by Micromelalopha sieversi (Staudinger) (Lepidoptera: Notodontidae). Materials and Methods: The differences in gene expression of two Populus section Aigeiros clones (‘108’ (Populus euramericana ‘Guariento’) and ‘111’ × (Populus euramericana ‘Bellotto’)) were analyzed via high-throughput sequencing of oviposited, × neighboring, and control plants. Results: We obtained 304,526,107 reads, with an average length of 300 bp and a total size of 40.77 Gb. Differentially expressed genes (DEGs) in gene ontology terms of biological process, cellular component, and molecular function were mainly enriched in the “cell part”, “catalytic”, and “metabolic process” functions.
    [Show full text]
  • The Isolation, Genetic Characterisation And
    The isolation, genetic characterisation and biological activity of a South African Phthorimaea operculella granulovirus (PhopGV-SA) for the control of the Potato Tuber Moth, Phthorimaea operculella (Zeller) A thesis submitted in fulfilment of the requirements for the degree of MASTER OF SCIENCE At RHODES UNIVERSITY By MICHAEL DAVID JUKES February 2015 i Abstract The potato tuber moth, Phthorimaea operculella (Zeller), is a major pest of potato crops worldwide causing significant damage to both field and stored tubers. The current control method in South Africa involves chemical insecticides, however, there is growing concern on the health and environmental risks of their use. The development of novel biopesticide based control methods may offer a potential solution for the future of insecticides. In this study a baculovirus was successfully isolated from a laboratory population of P. operculella. Transmission electron micrographs revealed granulovirus-like particles. DNA was extracted from recovered occlusion bodies and used for the PCR amplification of the lef-8, lef- 9, granulin and egt genes. Sequence data was obtained and submitted to BLAST identifying the virus as a South African isolate of Phthorimaea operculella granulovirus (PhopGV-SA). Phylogenetic analysis of the lef-8, lef-9 and granulin amino acid sequences grouped the South African isolate with PhopGV-1346. Comparison of egt sequence data identified PhopGV-SA as a type II egt gene. A phylogenetic analysis of egt amino acid sequences grouped all type II genes, including PhopGV-SA, into a separate clade from types I, III, IV and V. These findings suggest that type II may represent the prototype structure for this gene with the evolution of types I, III and IV a result of large internal deletion events and subsequent divergence.
    [Show full text]
  • Biodiversity, Evolution and Ecological Specialization of Baculoviruses: A
    Biodiversity, Evolution and Ecological Specialization of Baculoviruses: A Treasure Trove for Future Applied Research Julien Thézé, Carlos Lopez-Vaamonde, Jenny Cory, Elisabeth Herniou To cite this version: Julien Thézé, Carlos Lopez-Vaamonde, Jenny Cory, Elisabeth Herniou. Biodiversity, Evolution and Ecological Specialization of Baculoviruses: A Treasure Trove for Future Applied Research. Viruses, MDPI, 2018, 10 (7), pp.366. 10.3390/v10070366. hal-02140538 HAL Id: hal-02140538 https://hal.archives-ouvertes.fr/hal-02140538 Submitted on 26 May 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution| 4.0 International License viruses Article Biodiversity, Evolution and Ecological Specialization of Baculoviruses: A Treasure Trove for Future Applied Research Julien Thézé 1,2, Carlos Lopez-Vaamonde 1,3 ID , Jenny S. Cory 4 and Elisabeth A. Herniou 1,* ID 1 Institut de Recherche sur la Biologie de l’Insecte, UMR 7261, CNRS—Université de Tours, 37200 Tours, France; [email protected] (J.T.); [email protected]
    [Show full text]
  • Contribution to the Knowledge of the Fauna of Bombyces, Sphinges And
    driemaandelijks tijdschrift van de VLAAMSE VERENIGING VOOR ENTOMOLOGIE Afgiftekantoor 2170 Merksem 1 ISSN 0771-5277 Periode: oktober – november – december 2002 Erkenningsnr. P209674 Redactie: Dr. J–P. Borie (Compiègne, France), Dr. L. De Bruyn (Antwerpen), T. C. Garrevoet (Antwerpen), B. Goater (Chandlers Ford, England), Dr. K. Maes (Gent), Dr. K. Martens (Brussel), H. van Oorschot (Amsterdam), D. van der Poorten (Antwerpen), W. O. De Prins (Antwerpen). Redactie-adres: W. O. De Prins, Nieuwe Donk 50, B-2100 Antwerpen (Belgium). e-mail: [email protected]. Jaargang 30, nummer 4 1 december 2002 Contribution to the knowledge of the fauna of Bombyces, Sphinges and Noctuidae of the Southern Ural Mountains, with description of a new Dichagyris (Lepidoptera: Lasiocampidae, Endromidae, Saturniidae, Sphingidae, Notodontidae, Noctuidae, Pantheidae, Lymantriidae, Nolidae, Arctiidae) Kari Nupponen & Michael Fibiger [In co-operation with Vladimir Olschwang, Timo Nupponen, Jari Junnilainen, Matti Ahola and Jari- Pekka Kaitila] Abstract. The list, comprising 624 species in the families Lasiocampidae, Endromidae, Saturniidae, Sphingidae, Notodontidae, Noctuidae, Pantheidae, Lymantriidae, Nolidae and Arctiidae from the Southern Ural Mountains is presented. The material was collected during 1996–2001 in 10 different expeditions. Dichagyris lux Fibiger & K. Nupponen sp. n. is described. 17 species are reported for the first time from Europe: Clostera albosigma (Fitch, 1855), Xylomoia retinax Mikkola, 1998, Ecbolemia misella (Püngeler, 1907), Pseudohadena stenoptera Boursin, 1970, Hadula nupponenorum Hacker & Fibiger, 2002, Saragossa uralica Hacker & Fibiger, 2002, Conisania arida (Lederer, 1855), Polia malchani (Draudt, 1934), Polia vespertilio (Draudt, 1934), Polia altaica (Lederer, 1853), Mythimna opaca (Staudinger, 1899), Chersotis stridula (Hampson, 1903), Xestia wockei (Möschler, 1862), Euxoa dsheiron Brandt, 1938, Agrotis murinoides Poole, 1989, Agrotis sp.
    [Show full text]
  • SPG2: Biodiversity Conservation (July 2006) 1 1.0 an OVERVIEW
    Kent and Medway Structure Plan 2006 mapping out the future Supplementary Planning Guidance SPG2 Biodiversity Conservation July 2006 Strategy and Planning Division/ Environment and Waste Division Environment and Regeneration Directorate Kent County Council Tel: 01622 221609 Email: [email protected] Kent and Medway Structure Plan 2006 Supplementary Planning Guidance (SPG2): Biodiversity Conservation Preface i. The purpose of Supplementary Planning Guidance (SPG) is to supplement the policies and proposals of development plans. It elaborates policies so that they can be better understood and effectively applied. SPG should be clearly cross-referenced to the relevant plan policy or policies which it supplements and should be the subject of consultation during its preparation. In these circumstances SPG may be taken into account as a material consideration in planning decisions. ii. A number of elements of SPG have been produced to supplement certain policies in the Kent and Medway Structure Plan. This SPG supplements the following policies: • Policy EN6: International and National Wildlife Designations • Policy EN7: County and Local Wildlife Designations • Policy EN8: Protecting, Conserving and Enhancing Biodiversity • Policy EN9: Trees, Woodland and Hedgerows iii. This SPG has been prepared by Kent County Council working in partnership with a range of stakeholders drawn from Kent local authorities and other relevant agencies. iv. A draft of this SPG was subject to public consultation alongside public consultation on the deposit draft of the Kent and Medway Structure Plan in late 2003. It has been subsequently revised and updated prior to its adoption. A separate report provides a statement of the consultation undertaken, the representations received and the response to these representations.
    [Show full text]
  • The Complete Mitogenome of €Lysmata Vittata
    bioRxiv preprint doi: https://doi.org/10.1101/2021.08.04.455109; this version posted August 5, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 The complete mitogenome of Lysmata vittata (Crustacea: 2 Decapoda: Hippolytidae) and its phylogenetic position in 3 Decapoda 4 Longqiang Zhu1,2,3, Zhihuang Zhu1,2*, Leiyu Zhu1,2, Dingquan Wang3, Jianxin 5 Wang3*, Qi Lin1,2,3* 6 1 Fisheries Research Institute of Fujian, Xiamen, China 7 2 Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen, 8 China 9 3Marine Microorganism Ecological & Application Lab, Zhejiang Ocean University, Zhejiang, China 10 * [email protected] (QL); [email protected] (JW); [email protected] (ZZ) 11 12 Abstract 13 In this study, the complete mitogenome of Lysmata vittata (Crustacea: Decapoda: 14 Hippolytidae) has been determined. The genome sequence was 22003 base pairs (bp) 15 and it included thirteen protein-coding genes (PCGs), twenty-two transfer RNA genes 16 (tRNAs), two ribosomal RNA genes (rRNAs) and three putative control regions 17 (CRs). The nucleotide composition of AT was 71.50%, with a slightly negative AT 18 skewness (-0.04). Usually the standard start codon of the PCGs was ATN, while cox1, 19 nad4L and cox3 began with TTG, TTG and GTG. The canonical termination codon 20 was TAA, while nad5 and nad4 ended with incomplete stop codon T, and cox1 ended 21 with TAG.
    [Show full text]
  • Effects of Exogenous Methyl Jasmonate-Induced Resistance In
    Effects of exogenous methyl jasmonate-induced resistance in Populus × euramericana ‘Nanlin895’ on the performance and metabolic enzyme activities of Clostera anachoreta Gu Tianzi, Zhang Congcong, Chen Changyu, Li hui, Huang kairu, Tian Shuo, Zhao Xudong & Hao Dejun Arthropod-Plant Interactions An international journal devoted to studies on interactions of insects, mites, and other arthropods with plants ISSN 1872-8855 Volume 12 Number 2 Arthropod-Plant Interactions (2018) 12:247-255 DOI 10.1007/s11829-017-9564-y 1 23 Your article is protected by copyright and all rights are held exclusively by Springer Science+Business Media B.V.. This e-offprint is for personal use only and shall not be self- archived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com”. 1 23 Author's personal copy Arthropod-Plant Interactions (2018) 12:247–255 https://doi.org/10.1007/s11829-017-9564-y ORIGINAL PAPER Effects of exogenous methyl jasmonate-induced resistance in Populus 3 euramericana ‘Nanlin895’ on the performance and metabolic enzyme activities of Clostera anachoreta 1,2 1,2 1,2 1,2 1,2 Gu Tianzi • Zhang Congcong • Chen Changyu • Li hui • Huang kairu • 1,2 1,2 1,2 Tian Shuo • Zhao Xudong • Hao Dejun Received: 8 October 2016 / Accepted: 7 September 2017 / Published online: 21 September 2017 Ó Springer Science+Business Media B.V.
    [Show full text]
  • Midgut Transcriptome Analysis of Clostera Anachoreta Treated with Cry1ac Toxin
    bioRxiv preprint doi: https://doi.org/10.1101/568337; this version posted March 5, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Midgut Transcriptome Analysis of Clostera anachoreta Treated with Cry1Ac Toxin Liu Jie, Wang Liucheng, Zhou Guona, Liu Junxia, Gao Baojia Forestry College, Agricultural University of Hebei, 071000, Baoding, PR China. Correspondence and requests for materials should be addressed to (email: [email protected]) Abstract: Clostera anachoreta is one of the important Lepidoptera insect pests in forestry, especially in poplars woods in China, Europe, Japan and India, et al, and also the target insect of Cry1Ac toxin and Bt plants. In this study, by using the different dosages of Btcry1Ac toxin to feed larvae and analyzing the transcriptome data, we found six genes, HSC70, GNB2L/RACK1, PNLIP, BI1-like, arylphorin type 2 and PKM, might be associated with Cry1Ac toxin. And PI3K-Akt pathway, which was highly enriched in DEGs and linked to several crucial pathways, including the B cell receptor signaling pathway, toll-like receptor pathway, and MAPK signaling pathway, might be involved in the recovery stage when response to sub-lethal Cry1Ac toxin. This is the first study conducted to specifically investigate C. anachoreta response to Cry toxin stress using large-scale sequencing technologies, and the results highlighted some important genes and pathway that could be involved in Btcry1Ac resistance development or could serve as targets for biologically-based control mechanisms of this insect pest.
    [Show full text]