12Th Meeting of the European Association for Forensic Entomology 2015
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Do Longer Sequences Improve the Accuracy of Identification of Forensically Important Calliphoridae Species?
Do longer sequences improve the accuracy of identification of forensically important Calliphoridae species? Sara Bortolini1, Giorgia Giordani2, Fabiola Tuccia2, Lara Maistrello1 and Stefano Vanin2 1 Department of Life Sciences, University of Modena and Reggio Emilia, Reggio Emilia, Italy 2 School of Applied Sciences, University of Huddersfield, Huddersfield, United Kingdom ABSTRACT Species identification is a crucial step in forensic entomology. In several cases the calculation of the larval age allows the estimation of the minimum Post-Mortem Interval (mPMI). A correct identification of the species is the first step for a correct mPMI estimation. To overcome the difficulties due to the morphological identification especially of the immature stages, a molecular approach can be applied. However, difficulties in separation of closely related species are still an unsolved problem. Sequences of 4 different genes (COI, ND5, EF-1α, PER) of 13 different fly species collected during forensic experiments (Calliphora vicina, Calliphora vomitoria, Lu- cilia sericata, Lucilia illustris, Lucilia caesar, Chrysomya albiceps, Phormia regina, Cyno- mya mortuorum, Sarcophaga sp., Hydrotaea sp., Fannia scalaris, Piophila sp., Megaselia scalaris) were evaluated for their capability to identify correctly the species. Three concatenated sequences were obtained combining the four genes in order to verify if longer sequences increase the probability of a correct identification. The obtained results showed that this rule does not work for the species L. caesar and L. illustris. Future works on other DNA regions are suggested to solve this taxonomic issue. Subjects Entomology, Taxonomy Submitted 19 March 2018 Keywords ND5, COI, PER, Diptera, EF-1α, Maximum-likelihood, Phylogeny Accepted 17 October 2018 Published 17 December 2018 Corresponding author INTRODUCTION Stefano Vanin, [email protected] Species identification is a crucial step in forensic entomology. -
Dermestidae) Z Území Česka a Slovenska
Elateridarium 14: 174-193, 26.3.2020 ISSN 1802-4858 http://www.elateridae.com/elateridarium Příspěvek k poznání brouků čeledi kožojedovití (Dermestidae) z území Česka a Slovenska A contribution to the knowledge of Dermestidae (Coleoptera) from the Czechia and Slovakia Jiří HÁVA Private Entomological Laboratory and Collection, Rýznerova 37, CZ - 252 62 Únětice u Prahy, Praha-západ, Czechia e-mail: [email protected] Abstract. The new faunistics data for 31 species belonged to family Dermestidae (Coleoptera) known from Czechia and Slovakia are summarized. The two species Anthrenus (Nathrenus) signatus Erichson, 1846 and Anthrenus (Anthrenus) flavipes flavipes LeConte, 1854 are newly recorded from the Czechia (Moravia), species Trogoderma granarium Everts, 1898 is newly recorded from Slovakia. The parasitism of Holepyris sylvanidis (Brethes, 1913) (Hymenoptera: Bethylidae) on Trogoderma angustum (Solier, 1849) from the Czechia is recorded for the first time. Check-list of recorded species is attached. Key words: faunistics, new records, check-list, Coleoptera, Dermestidae, Czechia, Slovakia. Úvod Čeleď Dermestidae (kožojedovití) (Coleoptera) v současné době zahrnuje v celosvětovém měřítku celkem 1690 validních druhů a poddruhů (Háva 2020). Čeleď je na území Česka a Slovenska recentně studována, kromě souborné práce včetně určovacích klíčů publikované Hávou (2011), byla publikována i řada faunistických prací. V této práci autor předkládá nově zjištěné poznatky o faunistice 31 druhů z této čeledi z území Česka a Slovenska. Materiál a Metodika -
Life Science Journal 2017;14(3)
Life Science Journal 2017;14(3) http://www.lifesciencesite.com Identification of forensically important beetles on exposed human corpse in Jeddah, Kingdom of Saudi Arabia Layla A.H. Al-shareef1and Mammdouh K.Zaki2 1Faculty of Science-Al Faisaliah, King Abdulaziz University, Ministry of Education,Jeddah, Kingdom of Saudi Arabia. 2Forensic Medicine Center, Jeddah, Kingdom of Saudi Arabia [email protected],[email protected] Abstract: This study described beetle species attracted to an exposed human corpse in the decomposition stage between advanced decay and skeletal. The corpse was found during summer season in Jeddah city, the west region of the Kingdom of Saudi Arabia. Two families of Coleoptera were detected to colonize the corpse, they were Dermestidae represented by Dermestes frischii and Cleridae including Necrobia rufipes. The collected stages of beetles were described and photographed. The present work is the first documentation of these two species of beetles on human corpse for Jeddah city, kingdom of Saudi Arabia. [Layla A. Al-shareef and Mammdouh K.Zaki. Identification of forensically important beetles on exposed human corpse in Jeddah, Kingdom of Saudi Arabia. Life Sci J2017;14(3):28-38].ISSN: 1097-8135 (Print) /ISSN: 2372-613X (Online).http://www.lifesciencesite.com. 5.doi:10.7537/marslsj140317.05. Key words: forensic entomology, Jeddah, beetles, Dermestes frischii, Necrobia rufipes. Introduction: However, Family Cleridae has been recognized Among the arthropods visiting corpses or as multiple taxa. Most species are brightly colored. carrions, class Insecta is clearly prevalent with Diptera The subfamilies are rather varied in appearance and Coleoptera being the most abundant orders (1). compared to other families of Coleoptera (20). -
A Mummified Human Corpse and Associated Insects of Forensic Importance in Indoor Conditions
International Journal of Legal Medicine (2020) 134:1963–1971 https://doi.org/10.1007/s00414-020-02373-2 CASE REPORT A mummified human corpse and associated insects of forensic importance in indoor conditions Marcin Kadej1 & Łukasz Szleszkowski2 & Agata Thannhäuser2 & Tomasz Jurek2 Received: 17 March 2020 /Accepted: 8 July 2020 / Published online: 14 July 2020 # The Author(s) 2020 Abstract We report, for the first time from Poland, the presence of Dermestes haemorrhoidalis (Coleoptera: Dermestidae) on a mummified human corpse found in a flat (Lower Silesia province, south-western Poland). Different life stages of D. haemorrhoidalis were gathered from the cadaver, and the signs of activity of these beetles (i.e. frass) were observed. On the basis of these facts, we concluded that the decedent, whose remains were discovered in the flat on 13 December 2018, died no later than the summer of 2018, with a strong probability that death occurred even earlier (2016 or 2017). A case history, autopsy findings, and entomological observations are provided. The presence of larvae of Dermestidae in the empty puparia of flies is reported for the first time. A list of the invertebrate species found in the corpse is provided, compared with available data, and briefly discussed. Keywords Decomposition . Forensic entomology . Dermestes . Insects, mummified human corpse . Indoor Introduction Dermestidae) on a corpse found in a flat in Wrocław (Lower Silesia province, south-western Poland). In addition to the Among a large number of experimental papers, all highly identification of this species, we describe the interesting be- advanced in both methodical and technical terms are also pa- haviour of final-stage larvae associated with the use of empty pers describing specific cases relating to observations of in- casings of flies, probably as shelters for future pupae. -
Taxonomy and Systematics of the Australian Sarcophaga S.L. (Diptera: Sarcophagidae) Kelly Ann Meiklejohn University of Wollongong
University of Wollongong Research Online University of Wollongong Thesis Collection University of Wollongong Thesis Collections 2012 Taxonomy and systematics of the Australian Sarcophaga s.l. (Diptera: Sarcophagidae) Kelly Ann Meiklejohn University of Wollongong Recommended Citation Meiklejohn, Kelly Ann, Taxonomy and systematics of the Australian Sarcophaga s.l. (Diptera: Sarcophagidae), Doctor of Philosophy thesis, School of Biological Sciences, University of Wollongong, 2012. http://ro.uow.edu.au/theses/3729 Research Online is the open access institutional repository for the University of Wollongong. For further information contact the UOW Library: [email protected] Taxonomy and systematics of the Australian Sarcophaga s.l. (Diptera: Sarcophagidae) A thesis submitted in fulfillment of the requirements for the award of the degree Doctor of Philosophy from University of Wollongong by Kelly Ann Meiklejohn BBiotech (Adv, Hons) School of Biological Sciences 2012 Thesis Certification I, Kelly Ann Meiklejohn declare that this thesis, submitted in fulfillment of the requirements for the award of Doctor of Philosophy, in the School of Biological Sciences, University of Wollongong, is wholly my own work unless otherwise referenced or acknowledged. The document has not been submitted for qualifications at any other academic institution. Kelly Ann Meiklejohn 31st of August 2012 ii Table of Contents List of Figures .................................................................................................................................................. -
Insect Timing and Succession on Buried Carrion in East Lansing, Michigan
INSECT TIMING AND SUCCESSION ON BURIED CARRION IN EAST LANSING, MICHIGAN By Emily Christine Pastula A THESIS Submitted to Michigan State University in partial fulfillment of the requirements for the degree of MASTERS OF SCIENCE Entomology 2012 ABSTRACT INSECT TIMING AND SUCCESSION ON BURIED CARRION IN EAST LANSING, MICHIGAN By Emily Christine Pastula This study examined pig carcasses buried at two different depths, 30 and 60 cm, to determine if insects are able to colonize buried carcasses, when they arrive at each depth, and what fauna are present over seven sampling dates to establish an insect succession database on buried carrion in East Lansing, Michigan. Thirty-eight pigs were buried, 18 at 30 cm and 20 at 60 cm. Four control carcasses were placed on the soil surface. Three replicates at each depth were exhumed after 3 days, 7 days, 14 days, 21 days, 30 days, and 60 days. One pig was also exhumed from 60 cm after 90 days and another after 120 days. Sarcophaga bullata (Diptera: Sarcophagidae) and Hydrotaea sp. (Diptera: Muscidae) were found colonizing buried carrion 5 days after burial at 30 cm. Insect succession at 30 cm proceeded with flesh and muscid flies being the first to colonize, followed by blow flies. Insects were able to colonize carcasses at 60 cm and Hydrotaea sp. and Megaselia scalaris (Diptera: Phoridae), were collected 7 days after burial. Insect succession at 60 cm did not proceed similarly as predicted, instead muscid and coffin flies were the only larvae collected. Overall these results reveal post-burial interval (PBI) estimates for forensic investigations in mid-Michigan during the summer, depending on climatic and soil conditions. -
Lancs & Ches Muscidae & Fanniidae
The Diptera of Lancashire and Cheshire: Muscoidea, Part I by Phil Brighton 32, Wadeson Way, Croft, Warrington WA3 7JS [email protected] Version 1.0 21 December 2020 Summary This report provides a new regional checklist for the Diptera families Muscidae and Fannidae. Together with the families Anthomyiidae and Scathophagidae these constitute the superfamily Muscoidea. Overall statistics on recording activity are given by decade and hectad. Checklists are presented for each of the three Watsonian vice-counties 58, 59, and 60 detailing for each species the number of occurrences and the year of earliest and most recent record. A combined checklist showing distribution by the three vice-counties is also included, covering a total of 241 species, amounting to 68% of the current British checklist. Biodiversity metrics have been used to compare the pre-1970 and post-1970 data both in terms of the overall number of species and significant declines or increases in individual species. The Appendix reviews the national and regional conservation status of species is also discussed. Introduction manageable group for this latest regional review. Fonseca (1968) still provides the main This report is the fifth in a series of reviews of the identification resource for the British Fanniidae, diptera records for Lancashire and Cheshire. but for the Muscidae most species are covered by Previous reviews have covered craneflies and the keys and species descriptions in Gregor et al winter gnats (Brighton, 2017a), soldierflies and (2002). There have been many taxonomic changes allies (Brighton, 2017b), the family Sepsidae in the Muscidae which have rendered many of the (Brighton, 2017c) and most recently that part of names used by Fonseca obsolete, and in some the superfamily Empidoidea formerly regarded as cases erroneous. -
The Temporal Occurrence of Flesh Flies (Diptera
Africa Invertebrates 58(1):The 1–8 (2017)temporal occurrence of flesh flies( Diptera, Sarcophagidae)... 1 doi: 10.3897/AfrInvertebr.58.9537 RESEARCH ARTICLE http://africaninvertebrates.pensoft.net The temporal occurrence of flesh flies (Diptera, Sarcophagidae) at carrion-baited traps in Grahamstown, South Africa Martin H. Villet1, Crystal Clitheroe1, Kirstin A. Williams1 1 Southern African Forensic Entomology Research Laboratory, Department of Zoology and Entomology, Rhodes University, Grahamstown, PO Box 94, 6140 South Africa Corresponding author: Martin H. Villet ([email protected]) Academic editor: B. Muller | Received 13 June 2016 | Accepted 5 January 2017 | Published 18 January 2017 http://zoobank.org/61C10542-0AB9-4A14-B597-A4E051A585AC Citation: Villet MH, Clitheroe C, Williams KA (2017) The temporal occurrence of flesh flies (Diptera, Sarcophagidae) at carrion-baited traps in Grahamstown, South Africa. African Invertebrates 58(1): 1–8. doi: 10.3897/AfrInvertebr.58.9537 Abstract Eleven species of flesh fly were identified in a sample of 737 specimens captured during fortnightly trap- ping at three sites in Grahamstown, South Africa, over a year. Sarcophaga africa Wiedemann, 1824, S. inaequalis Austen, 1909, S. exuberans Pandellé, 1896 and S. tibialis Macquart, 1851 showed well-defined peaks between early October 2001 and late April 2002, and only S. africa was trapped at other times of year. These peaks occurred when average minimum and maximum ambient air temperatures were above 12°C and 22°C, respectively, and showed no obvious relationship to rainfall. There were indications of population cycles in all of these species. Sarcophaga hera Zumpt, 1972, S. arno Curran, 1934, S. inzi Cur- ran, 1934, S. -
Diptera: Sarcophagidae) and Its Phylogenetic Implications
First mitogenome for the subfamily Miltogramminae (Diptera: Sarcophagidae) and its phylogenetic implications Yan, Liping; Zhang, Ming; Gao, Yunyun; Pape, Thomas; Zhang, Dong Published in: European Journal of Entomology DOI: 10.14411/eje.2017.054 Publication date: 2017 Document version Publisher's PDF, also known as Version of record Document license: CC BY Citation for published version (APA): Yan, L., Zhang, M., Gao, Y., Pape, T., & Zhang, D. (2017). First mitogenome for the subfamily Miltogramminae (Diptera: Sarcophagidae) and its phylogenetic implications. European Journal of Entomology, 114, 422-429. https://doi.org/10.14411/eje.2017.054 Download date: 24. Sep. 2021 EUROPEAN JOURNAL OF ENTOMOLOGYENTOMOLOGY ISSN (online): 1802-8829 Eur. J. Entomol. 114: 422–429, 2017 http://www.eje.cz doi: 10.14411/eje.2017.054 ORIGINAL ARTICLE First mitogenome for the subfamily Miltogramminae (Diptera: Sarcophagidae) and its phylogenetic implications LIPING YAN 1, 2, MING ZHANG 1, YUNYUN GAO 1, THOMAS PAPE 2 and DONG ZHANG 1, * 1 School of Nature Conservation, Beijing Forestry University, Beijing, China; e-mails: [email protected], [email protected], [email protected], [email protected] 2 Natural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark; e-mail: [email protected] Key words. Diptera, Calyptratae, Sarcophagidae, Miltogramminae, mitogenome, fl esh fl y, phylogeny Abstract. The mitochondrial genome of Mesomelena mesomelaena (Loew, 1848) is the fi rst to be sequenced in the fl esh fl y subfamily Miltogramminae (Diptera: Sarcophagidae). The 14,559 bp mitogenome contains 37 typical metazoan mitochondrial genes: 13 protein-coding genes, two ribosomal RNA genes and 22 transfer RNA genes, with the same locations as in the insect ground plan. -
Surveying for Terrestrial Arthropods (Insects and Relatives) Occurring Within the Kahului Airport Environs, Maui, Hawai‘I: Synthesis Report
Surveying for Terrestrial Arthropods (Insects and Relatives) Occurring within the Kahului Airport Environs, Maui, Hawai‘i: Synthesis Report Prepared by Francis G. Howarth, David J. Preston, and Richard Pyle Honolulu, Hawaii January 2012 Surveying for Terrestrial Arthropods (Insects and Relatives) Occurring within the Kahului Airport Environs, Maui, Hawai‘i: Synthesis Report Francis G. Howarth, David J. Preston, and Richard Pyle Hawaii Biological Survey Bishop Museum Honolulu, Hawai‘i 96817 USA Prepared for EKNA Services Inc. 615 Pi‘ikoi Street, Suite 300 Honolulu, Hawai‘i 96814 and State of Hawaii, Department of Transportation, Airports Division Bishop Museum Technical Report 58 Honolulu, Hawaii January 2012 Bishop Museum Press 1525 Bernice Street Honolulu, Hawai‘i Copyright 2012 Bishop Museum All Rights Reserved Printed in the United States of America ISSN 1085-455X Contribution No. 2012 001 to the Hawaii Biological Survey COVER Adult male Hawaiian long-horned wood-borer, Plagithmysus kahului, on its host plant Chenopodium oahuense. This species is endemic to lowland Maui and was discovered during the arthropod surveys. Photograph by Forest and Kim Starr, Makawao, Maui. Used with permission. Hawaii Biological Report on Monitoring Arthropods within Kahului Airport Environs, Synthesis TABLE OF CONTENTS Table of Contents …………….......................................................……………...........……………..…..….i. Executive Summary …….....................................................…………………...........……………..…..….1 Introduction ..................................................................………………………...........……………..…..….4 -
Forensically Important Muscidae (Diptera) Associated with Decomposition of Carcasses and Corpses in the Czech Republic
MENDELNET 2016 FORENSICALLY IMPORTANT MUSCIDAE (DIPTERA) ASSOCIATED WITH DECOMPOSITION OF CARCASSES AND CORPSES IN THE CZECH REPUBLIC VANDA KLIMESOVA1, TEREZA OLEKSAKOVA1, MIROSLAV BARTAK1, HANA SULAKOVA2 1Department of Zoology and Fisheries Czech University of Life Sciences Prague (CULS) Kamycka 129, 165 00 Prague 6 – Suchdol 2Institute of Criminalistics Prague (ICP) post. schr. 62/KUP, Strojnicka 27, 170 89 Prague 7 CZECH REPUBLIC [email protected] Abstract: In years 2011 to 2015, three field experiments were performed in the capital city of Prague to study decomposition and insect colonization of large cadavers in conditions of the Central Europe. Experiments in turns followed decomposition in outdoor environments with the beginning in spring, summer and winter. As the test objects a cadaver of domestic pig (Sus scrofa f. domestica Linnaeus, 1758) weighing 50 kg to 65 kg was used for each test. Our paper presents results of family Muscidae, which was collected during all three studies, with focusing on its using in forensic practice. Altogether 29,237 specimens of the muscids were collected, which belonged to 51 species. It was 16.6% (n = 307) of the total number of Muscidae family which are recorded in the Czech Republic. In all experiments the species Hydrotaea ignava (Harris, 1780) was dominant (spring = 75%, summer = 81%, winter = 41%), which is a typical representative of necrophagous fauna on animal cadavers and human corpses in outdoor habitats during second and/or third successional stages (active decay phase) in the Czech Republic. Key Words: Muscidae, Diptera, forensic entomology, pyramidal trap INTRODUCTION Forensic or criminalistic entomology is the science discipline focusing on specific groups of insect for forensic and law investigation needs (Eliášová and Šuláková 2012). -
A Review of Stored-Product Entomology Information Sources
ResearcH Entomology Information Sources A Review of Stored-Product David W. Hagstrum and Bhadriraju Subramanyam ABSTRACT multibillion Thedollar field grain, of stored-product food, and retail entomology industries each deals year with through insect theirpests feeding, of raw and product processed adulteration, cereals, customer pulses, seeds, complaints, spices, productdried fruit rejection and nuts, at andthe timeother of dry, sale, durable and cost commodities. associated withThese their pests management. cause significant The reductionquantitative in theand number qualitative of stored-prod losses to the- pests is making full use of the literature on stored-product insects more important. Use of nonchemical and reduced-risk pest uct entomologists at a time when regulations are reducing the number of chemicals available to manage stored-product insect management methods requires a greater understanding of pest biology, behavior, ecology, and susceptibility to pest management methods. Stored-product entomology courses have been or are currently offered at land grant universities in four states in the United States and in at least nine other countries. Stored-product and urban entomology books cover the largest total numbers of stored-product insect species (100–160 and 24–120, respectively); economic entomology books (17–34), and popular articles or extension Web sites (29–52) cover fewer numbers of stored-product insect species. A review of 582 popular articles, 182 extension bulletins, and 226 extension Web sites showed that some aspects of stored-product entomology are covered better than others. For example, articles and Web sites on trapping (4.6%) and detection (3.3%) were more common than those on ofsampling an insect commodities pest management (0.6%).