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2Kcg Lichtarge Lab 2006 Pages 1–5 2kcg Evolutionary trace report by report maker July 21, 2010 4.3.3 DSSP 4 4.3.4 HSSP 4 4.3.5 LaTex 4 4.3.6 Muscle 4 4.3.7 Pymol 4 4.4 Note about ET Viewer 4 4.5 Citing this work 4 4.6 About report maker 5 4.7 Attachments 5 1 INTRODUCTION From the original Protein Data Bank entry (PDB id 2kcg): Title: Solution structure of cycloviolacin o2 Compound: Mol id: 1; molecule: cycloviolacin-o2; chain: a Organism, scientific name: Viola Odorata; 2kcg contains a single unique chain 2kcgA (30 residues long). This is an NMR-determined structure – in this report the first model in the file was used. 2 CHAIN 2KCGA 2.1 P58434 overview CONTENTS From SwissProt, id P58434, 100% identical to 2kcgA: 1 Introduction 1 Description: Cycloviolacin O2. Organism, scientific name: Viola odorata (Sweet violet). 2 Chain 2kcgA 1 Taxonomy: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; 2.1 P58434 overview 1 Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core 2.2 Multiple sequence alignment for 2kcgA 1 eudicotyledons; rosids; eurosids I; Malpighiales; Violaceae; Viola. 2.3 Residue ranking in 2kcgA 1 Function: Probably participates in a plant defense mechanism. Has 2.4 Top ranking residues in 2kcgA and their position on strong cytotoxic activity against a variety of drug-resistant and drug- the structure 2 sensitive human tumor cell lines, and against primary chronic lym- 2.4.1 Clustering of residues at 23% coverage. 2 phocytic leukemia and ovarian carcinoma cells. Has weaker cytotoxic 2.4.2 Possible novel functional surfaces at 23% activity against normal lymphocytes. coverage. 2 Ptm: This is a cyclic peptide. Similarity: Belongs to the cyclotide family. Bracelet subfamily. 3 Notes on using trace results 3 Caution: This peptide is cyclic, its sequence was chosen to start at 3.1 Coverage 3 the position shown below by similarity to Oak1 (kalata B1) whose 3.2 Known substitutions 3 DNA sequence is known. 3.3 Surface 3 About: This Swiss-Prot entry is copyright. It is produced through a 3.4 Number of contacts 3 collaboration between the Swiss Institute of Bioinformatics and the 3.5 Annotation 3 EMBL outstation - the European Bioinformatics Institute. There are 3.6 Mutation suggestions 3 no restrictions on its use as long as its content is in no way modified and this statement is not removed. 4 Appendix 3 4.1 File formats 3 2.2 Multiple sequence alignment for 2kcgA 4.2 Color schemes used 4 For the chain 2kcgA, the alignment 2kcgA.msf (attached) with 84 4.3 Credits 4 sequences was used. The alignment was downloaded from the HSSP 4.3.1 Alistat 4 database, and fragments shorter than 75% of the query as well as 4.3.2 CE 4 duplicate sequences were removed. It can be found in the attachment 1 Lichtarge lab 2006 Fig. 1. Residues 1-30 in 2kcgA colored by their relative importance. (See Appendix, Fig.5, for the coloring scheme.) to this report, under the name of 2kcgA.msf. Its statistics, from the alistat program are the following: Format: MSF Number of sequences: 84 Total number of residues: 2423 Smallest: 23 Largest: 30 Average length: 28.8 Alignment length: 30 Average identity: 68% Most related pair: 97% Most unrelated pair: 31% Most distant seq: 59% Fig. 2. Residues in 2kcgA, colored by their relative importance. Clockwise: front, back, top and bottom views. Furthermore, 16% of residues show as conserved in this alignment. The alignment consists of 16% eukaryotic ( 16% plantae) sequences. (Descriptions of some sequences were not readily availa- ble.) The file containing the sequence descriptions can be found in the attachment, under the name 2kcgA.descr. 2.3 Residue ranking in 2kcgA The 2kcgA sequence is shown in Fig. 1, with each residue colored according to its estimated importance. The full listing of residues in 2kcgA can be found in the file called 2kcgA.ranks sorted in the attachment. 2.4 Top ranking residues in 2kcgA and their position on the structure In the following we consider residues ranking among top 23% of resi- dues in the protein (the closest this analysis allows us to get to 25%). Figure 2 shows residues in 2kcgA colored by their importance: bright red and yellow indicate more conserved/important residues (see Appendix for the coloring scheme). A Pymol script for producing this figure can be found in the attachment. 2.4.1 Clustering of residues at 23% coverage. Fig. 3 shows the top 23% of all residues, this time colored according to clusters they belong to. The clusters in Fig.3 are composed of the residues listed in Table 1. Fig. 3. Residues in 2kcgA, colored according to the cluster they belong to: Table 1. red, followed by blue and yellow are the largest clusters (see Appendix for cluster size member the coloring scheme). Clockwise: front, back, top and bottom views. The corresponding Pymol script is attached. color residues red 7 4,8,12,13,20,22,27 Table 1. Clusters of top ranking residues in 2kcgA. susbtantially larger than) other functional sites and interfaces reco- gnizable in PDB entry 2kcg. It is shown in Fig. 4. The residues belonging to this surface ”patch” are listed in Table 2, while Table 2.4.2 Possible novel functional surfaces at 23% coverage. One 3 suggests possible disruptive replacements for these residues (see group of residues is conserved on the 2kcgA surface, away from (or Section 3.6). 2 Table 2. res type substitutions(%) cvg antn 8 C C(100) 0.17 S-S 13 C C(100) 0.17 S-S 20 C C(100) 0.17 S-S 22 C C(100) 0.17 S-S 27 C C(100) 0.17 S-S 12 P P(97)S(1)G(1) 0.20 4 C C(98).(1) 0.23 S-S Table 2. Residues forming surface ”patch” in 2kcgA. Table 3. res type disruptive mutations 8 C (KER)(FQMWHD)(NYLPI)(SVA) 13 C (KER)(FQMWHD)(NYLPI)(SVA) 20 C (KER)(FQMWHD)(NYLPI)(SVA) 22 C (KER)(FQMWHD)(NYLPI)(SVA) 27 C (KER)(FQMWHD)(NYLPI)(SVA) 12 P (R)(Y)(H)(K) Fig. 4. A possible active surface on the chain 2kcgA. 4 C (KER)(FQMWHD)(NLPI)(Y) Table 3. Disruptive mutations for the surface patch in 2kcgA. 3 NOTES ON USING TRACE RESULTS 3.1 Coverage Trace results are commonly expressed in terms of coverage: the resi- due is important if its “coverage” is small - that is if it belongs to some small top percentage of residues [100% is all of the residues in a chain], according to trace. The ET results are presented in the form of a table, usually limited to top 25% percent of residues (or to some nearby percentage), sorted by the strength of the presumed evolutionary pressure. (I.e., the smaller the coverage, the stronger the pressure on the residue.) Starting from the top of that list, mutating a couple of residues should affect the protein somehow, with the exact effects to be determined experimentally. 3.2 Known substitutions One of the table columns is “substitutions” - other amino acid types seen at the same position in the alignment. These amino acid types may be interchangeable at that position in the protein, so if one wants to affect the protein by a point mutation, they should be avoided. For example if the substitutions are “RVK” and the original protein has an R at that position, it is advisable to try anything, but RVK. Conver- sely, when looking for substitutions which will not affect the protein, one may try replacing, R with K, or (perhaps more surprisingly), with V. The percentage of times the substitution appears in the alignment is given in the immediately following bracket. No percentage is given in the cases when it is smaller than 1%. This is meant to be a rough guide - due to rounding errors these percentages often do not add up to 100%. 3.3 Surface To detect candidates for novel functional interfaces, first we look for residues that are solvent accessible (according to DSSP program) by 3 2 at least 10A˚ , which is roughly the area needed for one water mole- cule to come in the contact with the residue. Furthermore, we require that these residues form a “cluster” of residues which have neighbor within 5A˚ from any of their heavy atoms. Note, however, that, if our picture of protein evolution is correct, COVERAGE the neighboring residues which are not surface accessible might be equally important in maintaining the interaction specificity - they V should not be automatically dropped from consideration when choo- 100% 50% 30% 5% sing the set for mutagenesis. (Especially if they form a cluster with the surface residues.) 3.4 Number of contacts Another column worth noting is denoted “noc/bb”; it tells the num- ber of contacts heavy atoms of the residue in question make across V the interface, as well as how many of them are realized through the RELATIVE IMPORTANCE backbone atoms (if all or most contacts are through the backbone, mutation presumably won’t have strong impact). Two heavy atoms are considered to be “in contact” if their centers are closer than 5A˚ . Fig. 5. Coloring scheme used to color residues by their relative importance. 3.5 Annotation If the residue annotation is available (either from the pdb file or from other sources), another column, with the header “annotation” 2. their type appears. Annotations carried over from PDB are the following: site • rho ET score - the smaller this value, the lesser variability of (indicating existence of related site record in PDB ), S-S (disulfide this position across the branches of the tree (and, presumably, bond forming residue), hb (hydrogen bond forming residue, jb (james the greater the importance for the protein) bond forming residue), and sb (for salt bridge forming residue).
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