DOCSLIB.ORG

Double-Stranded RNA Binding Protein, Staufen, Is Required for the Initiation of Rnai in Coleopteran Insects

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Double-Stranded RNA Binding Protein, Staufen, Is Required for the Initiation of Rnai in Coleopteran Insects Double-stranded RNA binding protein, Staufen, is required for the initiation of RNAi in coleopteran insects June-Sun Yoona, Kanakachari Mogilicherlaa, Dhandapani Gurusamya, Xien Chena, Shankar C. R. R. Chereddya, and Subba Reddy Pallia,1 aDepartment of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY 40546 Edited by Lynn M. Riddiford, University of Washington, Friday Harbor, WA, and approved July 6, 2018 (received for review May 31, 2018) RNA interference (RNAi) is being used to develop methods to the main contributors to differential RNAi efficiency among control pests and disease vectors. RNAi is robust and systemic in insects (13–16). Recent studies identified dsRNA digestion by coleopteran insects but is quite variable in other insects. The dsRNases and entrapment of dsRNA in endosomes as the major determinants of efficient RNAi in coleopterans, as well as its potential contributors to RNAi recalcitrance in lepidopteran insects and mechanisms of resistance, are not known. RNAi screen identified a cell lines (17). Whether or not any coleopteran insect-specific double-stranded RNA binding protein (StaufenC) as a major player in proteins contribute to robust RNAi in these insects is not known. RNAi. StaufenC homologs have been identified in only coleopteran Cells defend themselves from dsRNAs from many sources insects. Experiments in two coleopteran insects, Leptinotarsa decem- (e.g., viruses). The enzyme Dicer-2 cleaves dsRNAs to small lineata and Tribolium castaneum, showed the requirement of interfering RNAs (siRNAs), which are then incorporated into an StaufenC for RNAi, especially for processing of double-stranded RNA RNA-induced silencing complex, bind to complementary (dsRNA) to small interfering RNA. RNAi-resistant cells were selected by mRNAs, and interfere with their translation (18, 19). Key pro- exposing L. decemlineata, Lepd-SL1 cells to the inhibitor of apoptosis 1 teins including Dicer-2, R2D2, Loquacious (Loqs), and Argo- dsRNA for multiple generations. The resistant cells showed lower levels nautes involved in RNAi pathway have been identified (20–22). of StaufenC expression compared with its expression in susceptible However, the difference in composition and function of silencing cells. These studies showed that coleopteran-specific StaufenC is re- complexes between insects in which RNAi works efficiently (e.g., quired for RNAi and is a potential target for RNAi resistance. The data coleopterans) and those in which RNAi does not work efficiently included in this article will help improve RNAi in noncoleopteran insects (e.g., lepidopterans) remains unknown. and manage RNAi resistance in coleopteran insects. Staufen was discovered in Drosophila melanogaster in a screen for maternal effect mutations (23). Molecular genetic studies Tribolium | Leptinotarsa | RNAi efficiency | resistance | siRNA have shown that D. melanogaster Staufen is required to localize mRNAs in oocytes and neuroblasts (24, 25). Human and mouse xposure of cells to double-stranded RNA (dsRNA) causes Staufen homologs were identified and the human staufen gene Etarget-specific gene silencing known as RNA interference codes for two proteins (Stau1 and Stau2) with differences in the (RNAi). RNAi was discovered in the nematode, Caenorhabditis elegans, and was subsequently observed in most eukaryotes in- Significance cluding humans, plants, and insects (1). dsRNAs synthesized in vitro or in microorganisms (including bacteria, yeast, and algae) or plants The RNA interference (RNAi) discovered in nematodes has have been used to achieve gene silencing in insects (2–4). RNAi- contributed to major advances in basic and applied sciences. aided gene silencing methods helped to determine functions of RNAi-based methods are being developed for controlling pests genes identified by the genome, transcriptome, and proteome se- and disease vectors. RNAi is highly efficient and systemic in quencing and advance our understanding of the molecular basis of coleopteran insects, but not in other insects. The lower effi- many developmental and physiological processes including insect ciency of RNAi in economically important insects and concerns development, reproduction, behavior, and communication (5, 6). about resistance development are hindering the widespread RNAi technology is being employed to develop methods to control use of this technology. To address these problems, a RNAi- crop pests as well as vectors that transmit deadly diseases affecting sensitive Colorado potato beetle, Leptinotarsa decemlineata, humans, animals, and plants (2–4, 7, 8). RNAi is also used to and a cell line derived from this insect were used to identify a identify target sites for insecticide development, as well as to dsRNA-binding protein, StaufenC, as a major contributor to manage insecticide resistance in pests and disease vectors (7–9). RNAi and its resistance. Interestingly, StaufenC homologs are The US Environmental Protection Agency recently approved present in only coleopteran insects and are essential for effi- transgenic corn plants expressing dsRNA for the control of Western cient RNAi response and its resistance in these insects. corn rootworm in the United States (https://www.regulations.gov/ docket?D=EPA-HQ-OPP-2014-0293). Author contributions: J.S.Y., K.M., and S.R.P. designed research; J.S.Y., K.M., D.G., X.C., and S.C.R.R.C. performed research; J.S.Y., K.M., D.G., X.C., S.C.R.R.C., and S.R.P. analyzed RNAi efficiency is variable among insects tested so far. RNAi data; and J.S.Y. and S.R.P. wrote the paper. works very well and is systemic in beetles (Coleoptera), including The authors declare no conflict of interest. the red flour beetle (Tribolium castaneum), Colorado potato This article is a PNAS Direct Submission. beetle (Leptinotarsa decemlineata), and Western corn rootworm – This open access article is distributed under Creative Commons Attribution-NonCommercial- (Diabrotica virgifera virgifera)(710). In contrast, RNAi works NoDerivatives License 4.0 (CC BY-NC-ND). poorly in moths and butterflies belonging to the order Lepi- Data deposition: The sequence reported in this paper has been deposited in the GenBank doptera (11, 12). Differences in digestion of dsRNA by en- database (accession no. SRP150964). dogenous dsRNases (dsRNA endonucleases), in transport of 1To whom correspondence should be addressed. Email: [email protected]. dsRNA into cells and trafficking within cells, in processing of This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10. dsRNA to siRNA, and in expression of RNAi genes and com- 1073/pnas.1809381115/-/DCSupplemental. position of proteins coded by these genes have been identified as Published online July 30, 2018. 8334–8339 | PNAS | August 14, 2018 | vol. 115 | no. 33 www.pnas.org/cgi/doi/10.1073/pnas.1809381115 Downloaded by guest on October 1, 2021 N-terminal end (26). Staufen1 is the mammalian homolog of D. melanogaster Staufen and is thought to function in mRNA transport, translational control, and mRNA decay (27–29). The staufen protein contains multiple double-stranded RNA binding domains, and some of them are shown to bind dsRNA (26, 30). Insects have developed resistance to almost all synthetic chem- icals used for their control, resulting in a constant battle between humans and insects. It is possible that insects will develop resistance to RNAi-based technologies as well. L. decemlineata has developed resistance to almost all synthetic chemicals used for its control withinashortperiodof2–3 y after their introduction (31). There- fore, L. decemlineata could be a good model insect to study po- tential RNAi resistance. Resistance in dsRNA-treated insects might be developed by the selection of individuals with modification in genes coding for proteins functioning in the RNAi pathway. Al- Fig. 1. Coleopteran-specific StauC is a major contributor to RNAi pathway. ternatively, insects might develop resistance to dsRNAs by selection (A) Phenotypes of cells exposed to dsGFP or dsStauC followed by exposure to of individuals with mutations in the dsRNA target sites. Information second dsRNA (dsGFP or dsIAP). The Lepd-SL1 cells were exposed to the first on potential mechanisms of RNAi resistance is needed to make dsRNA (dsGFP or dsStauC) for 48 h, followed by the treatment with the progress in the widespread use of RNAi for controlling insect pests second dsRNA (dsGFP or dsIAP). Photographs were taken at 24 h after and disease vectors. We used L. decemlineata and a cell line derived treatment with the second dsRNA. The arrow points to the cells undergoing from this insect to identify proteins required for RNAi in co- apoptosis. (B) Comparison of processing of dsRNA to siRNA in Lepd-SL1 cells leopteran insects, as well as to study potential mechanisms of RNAi exposed to dsGFP (control) or dsStauC. The Lepd-SL1 cells seeded in six-well plates were incubated with dsGFP or dsStauC for 24 or 48 h. Then, the cells resistance. were exposed to 1.6 million cpm 32P-labeled dsGFP. At 48 h after the addi- Results tion of the second dsRNA, the cells were harvested, and total RNA was iso- lated and the RNA containing 2,000 cpm was resolved on 16% acrylamide- StauC Is a Major Contributor to RNAi. In a recent study, we screened urea gel. The gel was dried and analyzed using a phosphorImager. The first 50 genes in Lepd-SL1 cells and identified five genes (Argonaute- lane shows GFP dsRNA used as a marker, and the last lane shows dsRNA 1, Argonaute-2a, Argonaute-2b, Aubergine, and vha16) that are processed to siRNA in Lepd-SL1 cells used as a marker for siRNA. The arrows essential for RNAi (32). In these experiments, when Lepd-SL1 point to dsRNA and siRNA bands. (C) Phylogenetic tree of dsRNA-binding cells were exposed to StaufenC (named as StauC because of its proteins. Major clusters of proteins include Staufen (mammalian Stau1 and presence only in coleopteran insects) dsRNA for 24 h, followed Stau2, and insect Staufens from 10 insect orders, and coleopteran StaufenC), insect dsRNA binding proteins (R2D2 and Loquacious), and mammalian RNA by a second dsRNA targeting the gene coding for inhibitor of binding protein (TRBP2).
Load more

Recommended publications
  • Beetle Appreciation Diversity and Classification of Common Beetle Families Christopher E
    Beetle Appreciation Diversity and Classification of Common Beetle Families Christopher E. Carlton Louisiana State Arthropod Museum Coleoptera Families Everyone Should Know (Checklist) Suborder Adephaga Suborder Polyphaga, cont. •Carabidae Superfamily Scarabaeoidea •Dytiscidae •Lucanidae •Gyrinidae •Passalidae Suborder Polyphaga •Scarabaeidae Superfamily Staphylinoidea Superfamily Buprestoidea •Ptiliidae •Buprestidae •Silphidae Superfamily Byrroidea •Staphylinidae •Heteroceridae Superfamily Hydrophiloidea •Dryopidae •Hydrophilidae •Elmidae •Histeridae Superfamily Elateroidea •Elateridae Coleoptera Families Everyone Should Know (Checklist, cont.) Suborder Polyphaga, cont. Suborder Polyphaga, cont. Superfamily Cantharoidea Superfamily Cucujoidea •Lycidae •Nitidulidae •Cantharidae •Silvanidae •Lampyridae •Cucujidae Superfamily Bostrichoidea •Erotylidae •Dermestidae •Coccinellidae Bostrichidae Superfamily Tenebrionoidea •Anobiidae •Tenebrionidae Superfamily Cleroidea •Mordellidae •Cleridae •Meloidae •Anthicidae Coleoptera Families Everyone Should Know (Checklist, cont.) Suborder Polyphaga, cont. Superfamily Chrysomeloidea •Chrysomelidae •Cerambycidae Superfamily Curculionoidea •Brentidae •Curculionidae Total: 35 families of 131 in the U.S. Suborder Adephaga Family Carabidae “Ground and Tiger Beetles” Terrestrial predators or herbivores (few). 2600 N. A. spp. Suborder Adephaga Family Dytiscidae “Predacious diving beetles” Adults and larvae aquatic predators. 500 N. A. spp. Suborder Adephaga Family Gyrindae “Whirligig beetles” Aquatic, on water
    [Show full text]
  • Flanged Bombardier Beetles from Laos (Carabidae, Paussinae)
    Entomologica Basiliensia et Collectionis Frey 31 101–113 2009 ISSN 1661–8041 Flanged Bombardier Beetles from Laos (Carabidae, Paussinae) by Peter Nagel Abstract. The Paussinae of Laos were recently studied based on new material collected by the Natural History Museum Basel. Two species are described as being new to science, Lebioderus brancuccii sp.nov., and Paussus lanxangensis sp.nov., and two species are new records for Laos. All species are shown in drawings. To date nine species are known from Laos, four of which have been added by the NHMB collecting trips, and a fifth new record is based on other museum collections. Key words. Laos – Paussinae – Lebioderus – Paussus – taxonomy– new species – myrmecophiles – distribution records Introduction Within the Oriental Region, Indochina is less well explored concerning the insect fauna than the Indian Subcontinent. Within Indochina, Laos is the least explored country, especially when compared to the insect fauna of the adjacent regions of Thailand. In contrast to neighboring countries, Laos still harbour large areas of forest, with relatively little disturbance and the presence of pristine habitats. However, demographic increases combined with forest burning, clearing for cultivation, and logging are major current threats to the Laotian environment. Therefore there are strong concerns for the survival of the high and unique biodiversity of this country which is situated in the centre of the Indo-Burma Hotspot (MITTERMEIER et al. 2004). In order to contribute to the documentation of the Laotian insect fauna as a basis for furthering our understanding and consequentially the conservation efforts, Dr. Michel Brancucci, Natural History Museum Basel, has conducted collecting trips to Laos in 2003, 2004, 2007 and 2009.
    [Show full text]
  • Lesser Mealworm, Litter Beetle, Alphitobius Diaperinus (Panzer) (Insecta: Coleoptera: Tenebrionidae)1 James C
    EENY-367 Lesser Mealworm, Litter Beetle, Alphitobius diaperinus (Panzer) (Insecta: Coleoptera: Tenebrionidae)1 James C. Dunford and Phillip E. Kaufman2 Introduction encountered in stored products (Green 1980). The other known species in the United States, A. laevigatus (Fabricius) The lesser mealworm, Alphitobius diaperinus (Panzer), is or black fungus beetle, is less commonly encountered and a cosmopolitan general stored products pest of particular may also vector pathogens and parasites and occasionally importance as a vector and competent reservoir of several cause damage to poultry housing. poultry pathogens and parasites. It can also cause damage to poultry housing and is suspected to be a health risk to humans in close contact with larvae and adults. Adults can become a nuisance when they move en masse toward artificial lights generated by residences near fields where beetle-infested manure has been spread (Axtell 1999). Alphitobius diaperinus inhabits poultry droppings and litter and is considered a significant pest in the poultry industry. Numerous studies have been conducted on lesser meal- worm biology, physiology, and management. Lambkin (2001) conducted a thorough review of relevant scientific literature in reference to A. diaperinus and provides a good understanding of the biology, ecology and bionomics of the pest. Bruvo et al. (1995) conducted molecular work to determine satellite DNA variants on the chromosomes of A. diaperinus. Alphitobius diaperinus is a member of the tenebrionid tribe Alphitobiini (Doyen 1989), which comprises four genera worldwide (Aalbu et al. 2002). Two genera occur Figure 1. Adult male lesser mealworm, Alphitobius diaperinus (Panzer). in the United States, of which there are two species in the This specimen taken from Henderson County, North Carolina.
    [Show full text]
  • Coleoptera Identifying the Beetles
    6/17/2020 Coleoptera Identifying the Beetles Who we are: Matt Hamblin [email protected] Graduate of Kansas State University in Manhattan, KS. Bachelors of Science in Fisheries, Wildlife and Conservation Biology Minor in Entomology Began M.S. in Entomology Fall 2018 focusing on Entomology Education Who we are: Jacqueline Maille [email protected] Graduate of Kansas State University in Manhattan, KS with M.S. in Entomology. Austin Peay State University in Clarksville, TN with a Bachelors of Science in Biology, Minor Chemistry Began Ph.D. iin Entomology with KSU and USDA-SPIERU in Spring 2020 Focusing on Stored Product Pest Sensory Systems and Management 1 6/17/2020 Who we are: Isaac Fox [email protected] 2016 Kansas 4-H Entomology Award Winner Pest Scout at Arnold’s Greenhouse Distribution, Abundance and Diversity Global distribution Beetles account for ~25% of all life forms ~390,000 species worldwide What distinguishes a beetle? 1. Hard forewings called elytra 2. Mandibles move horizontally 3. Antennae with usually 11 or less segments exceptions (Cerambycidae Rhipiceridae) 4. Holometabolous 2 6/17/2020 Anatomy Taxonomically Important Features Amount of tarsi Tarsal spurs/ spines Antennae placement and features Elytra features Eyes Body Form Antennae Forms Filiform = thread-like Moniliform = beaded Serrate = sawtoothed Setaceous = bristle-like Lamellate = nested plates Pectinate = comb-like Plumose = long hairs Clavate = gradually clubbed Capitate = abruptly clubbed Aristate = pouch-like with one lateral bristle Nicrophilus americanus Silphidae, American Burying Beetle Counties with protected critical habitats: Montgomery, Elk, Chautauqua, and Wilson Red-tipped antennae, red pronotum The ecological services section, Kansas department of Wildlife, Parks, and Tourism 3 6/17/2020 Suborders Adephaga vs Polyphaga Families ~176 described families in the U.S.
    [Show full text]
  • Mitochondrial Genomes Resolve the Phylogeny of Adephaga
    1 Mitochondrial genomes resolve the phylogeny 2 of Adephaga (Coleoptera) and confirm tiger 3 beetles (Cicindelidae) as an independent family 4 Alejandro López-López1,2,3 and Alfried P. Vogler1,2 5 1: Department of Life Sciences, Natural History Museum, London SW7 5BD, UK 6 2: Department of Life Sciences, Silwood Park Campus, Imperial College London, Ascot SL5 7PY, UK 7 3: Departamento de Zoología y Antropología Física, Facultad de Veterinaria, Universidad de Murcia, Campus 8 Mare Nostrum, 30100, Murcia, Spain 9 10 Corresponding author: Alejandro López-López ([email protected]) 11 12 Abstract 13 The beetle suborder Adephaga consists of several aquatic (‘Hydradephaga’) and terrestrial 14 (‘Geadephaga’) families whose relationships remain poorly known. In particular, the position 15 of Cicindelidae (tiger beetles) appears problematic, as recent studies have found them either 16 within the Hydradephaga based on mitogenomes, or together with several unlikely relatives 17 in Geadeadephaga based on 18S rRNA genes. We newly sequenced nine mitogenomes of 18 representatives of Cicindelidae and three ground beetles (Carabidae), and conducted 19 phylogenetic analyses together with 29 existing mitogenomes of Adephaga. Our results 20 support a basal split of Geadephaga and Hydradephaga, and reveal Cicindelidae, together 21 with Trachypachidae, as sister to all other Geadephaga, supporting their status as Family. We 22 show that alternative arrangements of basal adephagan relationships coincide with increased 23 rates of evolutionary change and with nucleotide compositional bias, but these confounding 24 factors were overcome by the CAT-Poisson model of PhyloBayes. The mitogenome + 18S 25 rRNA combined matrix supports the same topology only after removal of the hypervariable 26 expansion segments.
    [Show full text]
  • The Evolution and Genomic Basis of Beetle Diversity
    The evolution and genomic basis of beetle diversity Duane D. McKennaa,b,1,2, Seunggwan Shina,b,2, Dirk Ahrensc, Michael Balked, Cristian Beza-Bezaa,b, Dave J. Clarkea,b, Alexander Donathe, Hermes E. Escalonae,f,g, Frank Friedrichh, Harald Letschi, Shanlin Liuj, David Maddisonk, Christoph Mayere, Bernhard Misofe, Peyton J. Murina, Oliver Niehuisg, Ralph S. Petersc, Lars Podsiadlowskie, l m l,n o f l Hans Pohl , Erin D. Scully , Evgeny V. Yan , Xin Zhou , Adam Slipinski , and Rolf G. Beutel aDepartment of Biological Sciences, University of Memphis, Memphis, TN 38152; bCenter for Biodiversity Research, University of Memphis, Memphis, TN 38152; cCenter for Taxonomy and Evolutionary Research, Arthropoda Department, Zoologisches Forschungsmuseum Alexander Koenig, 53113 Bonn, Germany; dBavarian State Collection of Zoology, Bavarian Natural History Collections, 81247 Munich, Germany; eCenter for Molecular Biodiversity Research, Zoological Research Museum Alexander Koenig, 53113 Bonn, Germany; fAustralian National Insect Collection, Commonwealth Scientific and Industrial Research Organisation, Canberra, ACT 2601, Australia; gDepartment of Evolutionary Biology and Ecology, Institute for Biology I (Zoology), University of Freiburg, 79104 Freiburg, Germany; hInstitute of Zoology, University of Hamburg, D-20146 Hamburg, Germany; iDepartment of Botany and Biodiversity Research, University of Wien, Wien 1030, Austria; jChina National GeneBank, BGI-Shenzhen, 518083 Guangdong, People’s Republic of China; kDepartment of Integrative Biology, Oregon State
    [Show full text]
  • Colorado Potato Beetle.Pub
    CORNELL COOPERATIVE EXTENSION OF ONEIDA COUNTY 121 Second Street Oriskany, NY 13424-9799 (315) 736-3394 or (315) 337-2531 FAX: (315) 736-2580 Colorado Potato Beetle Leptinotarsa decemlineata Description: The Colorado potato beetle was first described in 1824 from the upper Missouri River Valley where it fed on a weed called buffalo bur or sand bur, but when early settlers first began to plant potatoes, the beetles discovered the new food plant and liked it. Adult Colorado Potato Beetle Colorado Potato Beetle larva Injury: Larvae and adults feed on the foliage of potato, eggplant, tomatoes and peppers. They may reach large numbers and eat all the foliage from the plant as well as spoil the fruit by eating into it. They are especially de- structive to small plantings. Life History: Adult beetles come out of winter hibernation in mid-May on Long Island and a week or ten days later in central New York just before the early-planted potatoes are up. Clusters of 20 or more eggs are laid on the underside of the leaves soon after the beetles emerge. The eggs hatch in seven to ten days. The larvae feed on the foliage, grow rapidly and complete their development in 18 to 21 days. The full-grown larva burrows into the ground and changes to the pupa or resting stage. After seven to ten days, the adult beetle emerges from the pupa, crawls up out of the ground, and after a short period of waiting, lays eggs for the second generation. Management: In the past several years, the Colorado potato beetle has become increasingly difficult to control because it has developed resistance to many commonly used chemical insecticides.
    [Show full text]
  • Susceptibility of Adult Colorado Potato Beetle (Leptinotarsa Decemlineata) to the Fungal Entomopathogen Beauveria Bassiana Ellen Klinger
    The University of Maine DigitalCommons@UMaine Electronic Theses and Dissertations Fogler Library 8-2003 Susceptibility of Adult Colorado Potato Beetle (Leptinotarsa Decemlineata) to the Fungal Entomopathogen Beauveria Bassiana Ellen Klinger Follow this and additional works at: http://digitalcommons.library.umaine.edu/etd Part of the Agricultural Science Commons, Agriculture Commons, Entomology Commons, and the Environmental Sciences Commons Recommended Citation Klinger, Ellen, "Susceptibility of Adult Colorado Potato Beetle (Leptinotarsa Decemlineata) to the Fungal Entomopathogen Beauveria Bassiana" (2003). Electronic Theses and Dissertations. 386. http://digitalcommons.library.umaine.edu/etd/386 This Open-Access Thesis is brought to you for free and open access by DigitalCommons@UMaine. It has been accepted for inclusion in Electronic Theses and Dissertations by an authorized administrator of DigitalCommons@UMaine. SUSCEPTIBILITY OF ADULT COLORADO POTATO BEETLE (LEPTINOTARSA DECEMLINEATA) TO THE FUNGAL ENTOMOPATHOGEN BEAUVERIA BASSIANA BY Ellen Klinger B.S. Lycoming College, 2000 A THESIS Submitted in Partial Fulfillment of the Requirements for the Degree of Master of Science (in Ecology and Environmental Sciences) The Graduate School The University of Maine August, 2003 Advisory Committee: Eleanor Groden, Associate Professor of Entomology, Advisor Francis Drumrnond, Professor of Entomology Seanna Annis, Assistant Professor of Mycology SUSCEPTIBILITY OF ADULT COLORADO POTATO BEETLE (LEPTINOTARSA DECEMLINEATA) TO THE FUNGAL ENTOMOPATHOGEN BEAUVERIA BASSIANA By Ellen Klinger Thesis Advisor: Dr. Eleanor Groden An Abstract of the Thesis Presented in Partial Fulfillment of the Requirements for the Degree of Master of Science (in Ecology and Environmental Sciences) August, 2003 Factors influencing the susceptibility of adult Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), to the fungal entomopathogen, Beauveria bassiana (Bals.), were studied.
    [Show full text]
  • Integrated Pest Management in the Global Arena
    Integrated Pest Management in the Global Arena This Handbook is dedicated to all the participants of the Michigan State University’s International IPM Short Course and to the faculty members who have provided support to this course. Integrated Pest Management in the Global Arena Edited by K.M. Maredia Professor Institute of International Agriculture and Department of Entomology Michigan State University East Lansing Michigan USA D. Dakouo Senior Research Scientist INERA Bobo-Dioulasso Burkina Faso and D. Mota-Sanchez Research Associate Department of Entomology Michigan State University East Lansing Michigan USA CABI Publishing CABI Publishing is a division of CAB International CABI Publishing CABI Publishing CAB International 44 Brattle Street Wallingford 4th Floor Oxon OX10 8DE Cambridge, MA 02138 UK USA Tel: +44 (0)1491 832111 Tel: +1 617 395 4056 Fax: +44 (0)1491 833508 Fax: +1 617 354 6875 Email: [email protected] E-mail: [email protected] Website: www.cabi-publishing.org ©CAB International 2003. All rights reserved. No part of this publication may be reproduced in any form or by any means, electronically, mechanically, by photocopying, recording or otherwise, without the prior permission of the copyright owners. A catalogue record for this book is available from the British Library, London, UK. Library of Congress Cataloging-in-Publication Data Integrated pest management in the global arena /edited by K.M. Maredia, D. Dakouo, D. Mota-Sanchez p. cm. Includes bibliographical references. ISBN 0-85199-652-3 1. Pests--Integrated control. I. Maredia, Karim M. II. Dakouo, D. (Dona), 1951- III. Mota-Sanchez, D. (David), 1960- SB950.I4575 2003 632′.9--dc21 2002154965 ISBN 0 85199 652 3 Typeset by AMA DataSet, UK Printed and bound in the UK by Cromwell Press, Trowbridge Contents Contributors ix Preface xv Acknowledgments xvii Foreword xix Acronyms and Abbreviations xxi 1Introduction and Overview 1 K.M.
    [Show full text]
  • Modern Techniques in Colorado Potato Beetle (Leptinotarsa Decemlineata Say) Control and Resistance Management: History Review and Future Perspectives
    insects Review Modern Techniques in Colorado Potato Beetle (Leptinotarsa decemlineata Say) Control and Resistance Management: History Review and Future Perspectives Martina Kadoi´cBalaško 1,* , Katarina M. Mikac 2 , Renata Bažok 1 and Darija Lemic 1 1 Department of Agricultural Zoology, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, Croatia; [email protected] (R.B.); [email protected] (D.L.) 2 Centre for Sustainable Ecosystem Solutions, School of Earth, Atmospheric and Life Sciences, Faculty of Science, Medicine and Health, University of Wollongong, Wollongong 2522, Australia; [email protected] * Correspondence: [email protected]; Tel.: +385-1-239-3654 Received: 8 July 2020; Accepted: 22 August 2020; Published: 1 September 2020 Simple Summary: The Colorado potato beetle (CPB) is one of the most important potato pest worldwide. It is native to U.S. but during the 20th century it has dispersed through Europe, Asia and western China. It continues to expand in an east and southeast direction. Damages are caused by larvae and adults. Their feeding on potato plant leaves can cause complete defoliation and lead to a large yield loss. After the long period of using only chemical control measures, the emergence of resistance increased and some new and different methods come to the fore. The main focus of this review is on new approaches to the old CPB control problem. We describe the use of Bacillus thuringiensis and RNA interference (RNAi) as possible solutions for the future in CPB management. RNAi has proven successful in controlling many pests and shows great potential for CPB control. Better understanding of the mechanisms that affect efficiency will enable the development of this technology and boost potential of RNAi to become part of integrated plant protection in the future.
    [Show full text]
  • The Evolution of Spinosad Resistance in Colorado Potato Beetles (Leptinotarsa Decemlineata)
    City University of New York (CUNY) CUNY Academic Works All Dissertations, Theses, and Capstone Projects Dissertations, Theses, and Capstone Projects 9-2019 The Evolution of Spinosad Resistance in Colorado Potato Beetles (Leptinotarsa decemlineata) Coby Michael Klein The Graduate Center, City University of New York How does access to this work benefit ou?y Let us know! More information about this work at: https://academicworks.cuny.edu/gc_etds/3464 Discover additional works at: https://academicworks.cuny.edu This work is made publicly available by the City University of New York (CUNY). Contact: [email protected] THE EVOLUTION OF SPINOSAD RESISTANCE IN COLORADO POTATO BEETLES (LEPTINOTARSA DECEMLINEATA) by COBY MICHAEL KLEIN A dissertation submitted to the Graduate Faculty in Biology in partial fulfillment of the requirements for the degree of Doctor of Philosophy, The City University of New York 2019 i © 2019 COBY MICHAEL KLEIN All Rights Reserved ii The Evolution of Spinosad Resistance in Colorado Potato Beetles (Leptinotarsa decemlineata) by Coby Michael Klein This manuscript has been read and accepted by the Graduate Faculty in Biology in satisfaction of the dissertation requirement for the degree of Doctor of Philosophy. _____________________ ________________________________________________ Date Mitchell Baker Chair of Examining Committee _____________________ ________________________________________________ Date Cathy Savage-Dunn Executive Officer Supervisory Committee Dr. Andrei Alyokhin Dr. Jose Anadon Dr. Amy Berkov Dr. David Lahti THE CITY UNIVERSITY OF NEW YORK iii ABSTRACT The Evolution of Spinosad Resistance in Colorado Potato Beetles (Leptinotarsa decemlineata) by Coby Michael Klein Advisor: Dr. Mitchell Baker Colorado potato beetles (Leptinotarsa decemlineata) are a major pest of cultivated potato plants worldwide.
    [Show full text]
  • Coleoptera: Adephaga: Dytiscidae, Gyrinidae) Collected Along the Horton and Thelon Rivers in the Arctic Central Barrens of Canada
    Predaceous Water Beetles (Coleoptera: Adephaga: Dytiscidae, Gyrinidae) Collected Along the Horton and Thelon Rivers in the Arctic Central Barrens of Canada HELENA V. S HAVERDO1 and DONNA J. GIBERSON2 1Department of Entomology, 213 Animal Science/Entomology Building, University of Manitoba, Winnipeg, Manitoba R3T 2N2 Canada 2Department of Biology, University of Prince Edward Island, Charlottetown, Prince Edward Island C1A 4P3 Canada Shaverdo, Helena V., and Donna J. Giberson. Predaceous water beetles (Coleoptera: Adephaga: Dytiscidae, Gyrinidae) col- lected along the Horton and Thelon rivers in the Arctic Central Barrens of Canada. Canadian Field-Naturalist 118(3): 425-433. Predaceous water beetles were collected during expeditions along two northern Canadian rivers during 2000 and 2002. Twelve species of Dytiscidae (including 11 named species and one additional genus identified from a larva) and one species of Gyrinidae are recorded from 20 sites along the Horton and Thelon rivers in the Central Barrens area of the Canadian Arctic. These records represent an extension of the distributions of four species to the northeast in the Northwest Territories (NWT), and two species to the northwest in Nunavut (NU). Oreodytes sanmarkii is reported for NWT and for the mainland of NU for the first time. Ilybius erichsoni, Hydroporus geniculatus, and Gyrinus opacus are reported for NU for the first time. Five species were recorded for the first time from the Southern Arctic ecozone, and one from the Taiga Shield ecozone. The majority of specimens were collected in habitats that were consistent with those previously known for each species. Key Words: Insecta, Coleoptera, Adephaga, Dytiscidae, Gyrinidae, predaceous water beetles, faunistics, river, Northwest Territories, Nunavut, Arctic Central Barrens.
    [Show full text]