The Lignan Macromolecule from Flaxseed Structure and Bioconversion of Lignans
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Chemoprotective Effects of Flaxseed Lignans Enterodiol And
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Texas A&M Repository CHEMOPROTECTIVE EFFECTS OF FLAXSEED LIGNANS ENTERODIOL AND ENTEROLACTONE IN NON-TRANSFORMED COLONOCYTES A Thesis by CHRISTINA ALISON CURRY Submitted to the Office of Graduate and Professional Studies of Texas A&M University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Chair of Committee, Clinton Allred Committee Members, Joseph Awika Jenna Anding Head of Department, Boon Chew December 2015 Major Subject: Nutrition Copyright 2015 Christina Alison Curry ABSTRACT Previous epidemiological studies have shown that colon cancer incidence is correlated to diet and estrogen status. Phytoestrogens are molecules with similar structures to estrogen that occur naturally in plants. There is in vitro and in vivo evidence that phytoestrogens in the diet can inhibit carcinogenesis. The phytoestrogenic mammalian lignans enterolactone (EL) and enterodiol (ED) in flaxseed have been shown to be effective in decreasing tumor incidence in carcinogenic models, but there is little data regarding their effects in non-malignant cells. The following studies used a non- transformed cell line of young adult mouse colonocytes (YAMC) to determine the protective effects of ED and EL in chemoprevention. Our results demonstrate that low levels of EL (1µM) and ED (5µM) are effective at significantly reducing cell growth and increasing apoptosis. These treatments also regulated transcription via significant differences in gene levels related to apoptosis and cell cycle progression. The data collected demonstrate some of the physiological effects of EL and ED on the cellular and molecular level. -
Lignan Accumulation in Two-Phase Cultures of Taxus X Media Hairy Roots
Plant Cell, Tissue and Organ Culture (PCTOC) https://doi.org/10.1007/s11240-018-1390-0 ORIGINAL ARTICLE Lignan accumulation in two-phase cultures of Taxus x media hairy roots K. Sykłowska‑Baranek1 · K. Łysik1 · M. Jeziorek1 · A. Wencel1 · M. Gajcy1 · A. Pietrosiuk1 Received: 3 August 2017 / Accepted: 6 February 2018 © The Author(s) 2018. This article is an open access publication Abstract The biosynthetic potential for six lignans accumulation in two lines of Taxus x media hairy roots was investigated. The cul- tures of KT and ATMA hairy root lines were supplemented with precursors: coniferyl alcohol (CA 1, 10 or 100 µM) and/or L-phenylalanine (100 µM PHEN) and/or methyl jasmonate (100 µM MeJa). Moreover the two-phase in vitro cultures sup- ported with perfluorodecalin (PFD) as a gas carrier and in situ extrahent were used. The hairy root lines differed in lignan production profiles. In the control untreated cultures KT roots did not accumulate secoisolariciresinol and lariciresinol while ATMA roots did not accumulate matairesinol. In ATMA roots the treatment with CA (1 or 10 µM) resulted in the production of lariciresinol and secoisolariciresinol whereas solely lariciresinol was present after 100 µM CA application. Elicitation with 1 µM CA and MeJa yielded with hydroxymatairesinol aglyca and lariciresinol glucosides with their highest content 37.88 and 3.19 µg/g DW, respectively. The stimulatory effect of simultaneous treatment with 1 µM CA, PHEN and MeJa on lignan production was observed when the cultures were supplemented with PFD-aerated or degassed. In ATMA root cultures these applied conditions were the most favourable for matairesinol content which amounted to 199.86 and 160.25 µg/g DW in PFD-aerated and PFD-degassed supported cultures, respectively. -
Pinoresinol Reductase 1 Impacts Lignin Distribution During Secondary Cell Wall Biosynthesis in Arabidopsis
Phytochemistry xxx (2014) xxx–xxx Contents lists available at ScienceDirect Phytochemistry journal homepage: www.elsevier.com/locate/phytochem Pinoresinol reductase 1 impacts lignin distribution during secondary cell wall biosynthesis in Arabidopsis Qiao Zhao a, Yining Zeng b,e, Yanbin Yin c, Yunqiao Pu d,e, Lisa A. Jackson a,e, Nancy L. Engle e,f, Madhavi Z. Martin e,f, Timothy J. Tschaplinski e,f, Shi-You Ding b,e, Arthur J. Ragauskas d,e, ⇑ Richard A. Dixon a,e,g, a Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA b Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA c Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA d Institute of Paper Science and Technology, Georgia Institute of Technology, Atlanta, GA, USA e BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA f Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA g Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA article info abstract Article history: Pinoresinol reductase (PrR) catalyzes the conversion of the lignan (À)-pinoresinol to (À)-lariciresinol in Available online xxxx Arabidopsis thaliana, where it is encoded by two genes, PrR1 and PrR2, that appear to act redundantly. PrR1 is highly expressed in lignified inflorescence stem tissue, whereas PrR2 expression is barely detect- Keywords: able in stems. Co-expression analysis has indicated that PrR1 is co-expressed with many characterized Lignan genes involved in secondary cell wall biosynthesis, whereas PrR2 expression clusters with a different Lignin set of genes. -
(12) Patent Application Publication (10) Pub. N0.: US 2014/0221426 A1 Gerk Et Al
US 20140221426A1 (19) United States (12) Patent Application Publication (10) Pub. N0.: US 2014/0221426 A1 Gerk et al. (43) Pub. Date: Aug. 7, 2014 (54) SELECTIVE METABOLIC APPROACH TO A61K 31/216 (2006.01) INCREASING ORAL BIOAVAILABILITY OF A61K 31/09 (2006.01) PHENYLEPHRINE AND OTHER PHENOLIC A61K 31/05 (2006.01) BIOACTIVITIES A61K 31/353 (2006.01) A61K 31/4525 (2006.01) (71) Applicant: VIRGINIA COMMONWEALTH A61 K 31/3 75 (2006.01) UNIVERSITY, Richmond, VA (US) A61K 31/121 (2006.01) _ _ _ (52) US. Cl. (72) Inventorsl Ph_lll_lP M- Gerk’ Rthmond, VA (Us); CPC ........... .. A61K 31/137 (2013.01); A61K 31/3 75 Wllllam H- Fa", R10hm°nda VA (Us); (2013.01); A61K 31/235 (2013.01); A61K J"sellh K- thter’ Rlchmond, VA (Us) 31/11 (2013.01); A61K 31/085 (2013.01); _ A61K 31/121 (2013.01); A61K 31/09 (21) APP1~ NO" 14/345,689 (2013.01); A61K31/05 (2013.01); A61K . _ 31/353 (2013.01);A61K31/4525 (2013.01); (22) PCT Filed. Sep. 27, 2012 A61K31/216 (201301) USPC ......... .. 514/321' 514/653' 514/474' 514/544' ( 86 ) PCT N 0 .: PCT/U52012/057588 ’ ’ 514/456;’ 514/532’ § 371 (0X1), Related US“ Application Data Presystemic metabolism in intestine of bioactives such as (60) Provisional application No. 61/539,530, ?led on Sep. phenylephrine 1? avoided by administering a Sllbject (human 27, 2011, provisional application No. 61/544,396, 0r 21111111211) the bloactlve(e-g-,Pheny1ephr1ne)1n comblnatlon ?led on Oct 7, 201 1_ With one or more inhibitors of sulfation (e.g., sulfotransferase enzymes aka SULTs). -
Nutraceuticals Brian Lockwood
CjigVXZji^XVah HZXdcYZY^i^dc 7g^VcAdX`lddY 00 Prelim 2/3/07 18:51 Page i Nutraceuticals 00 Prelim 2/3/07 18:51 Page ii 00 Prelim 2/3/07 18:51 Page iii Nutraceuticals A guide for healthcare professionals Second edition Brian Lockwood BPharm, PhD, MRPharmS Senior Lecturer in Pharmacy, School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester, UK London • Chicago 00 Prelim 2/3/07 18:51 Page iv Published by the Pharmaceutical Press An imprint of RPS Publishing 1 Lambeth High Street, London SE1 7JN, UK 100 South Atkinson Road, Suite 200, Grayslake, IL 60030–7820, USA © Pharmaceutical Press 2007 is a trade mark of RPS Publishing RPS Publishing is the publishing organisation of the Royal Pharmaceutical Society of Great Britain First edition published 2002 Second edition published 2007 Typeset by Type Study, Scarborough, North Yorkshire Printed in Great Britain by TJ International, Padstow, Cornwall ISBN 978 0 85369 659 9 All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, without the prior written permission of the copyright holder. The publisher makes no representation, express or implied, with regard to the accuracy of the information contained in this book and cannot accept any legal responsibility or liability for any errors or omissions that may be made. The right of Brian Lockwood to be identified as the author of this work has been asserted by him in accordance with sections 77 and 78 and subject to section 79(6) of the Copyright, Designs and Patents Act, 1988. -
Serum Enterolactone
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Julkari Annamari Kilkkinen SERUM ENTEROLACTONE D E T E R M I N A N T S A N D A S S O C I A T I O N S W I T H B R E A S T A N D P R O S T A T E C A N C E R S A C A D E M I C D I S S E R T A T I O N To be presented with the permission of the Faculty of Medicine, University of Helsinki, for public examination in Auditorium XII, University Main Building, on June 11th, 2004, at 12 noon. National Public Health Institute, Helsinki, Finland and Department of Public Health, University of Helsinki, Finland Helsinki 2004 P u b l i c a t i o n s o f t h e N a t i o n a l P u b l i c H e a l t h I n s t i t u t e K T L A 1 0 / 2 0 0 4 Copyright National Public Health Institute Julkaisija-Utgivare-Publisher Kansanterveyslaitos (KTL) Mannerheimintie 166 00300 Helsinki Puh. vaihde (09) 474 41, telefax (09) 4744 8408 Folkhälsoinstitutet Mannerheimvägen 166 00300 Helsingfors Tel. växel (09) 474 41, telefax (09) 4744 8408 National Public Health Institute Mannerheimintie 166 FIN-00300 Helsinki, Finland Telephone +358 9 474 41, telefax +358 9 4744 8408 ISBN 951-740-448-4 ISSN 0359-3584 ISBN 951-740-449-2 (pdf) ISSN 1458-6290 (pdf) Hakapaino Oy Helsinki 2004 S u p e r v i s e d b y Professor Pirjo Pietinen Department of Epidemiology and Health Promotion National Public Health Institute, Helsinki, Finland Professor Jarmo Virtamo Department of Epidemiology and Health Promotion National Public Health Institute, Helsinki, Finland R e v i e w e d b y Associate Professor Sari -
Chemical Structures of Lignans and Neolignans Isolated from Lauraceae
Review Chemical Structures of Lignans and Neolignans Isolated from Lauraceae Ya Li 1,*, Shuhan Xie 2, Jinchuan Ying 1, Wenjun Wei 1 and Kun Gao 1,* 1 State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China; [email protected] (J.Y.); [email protected] (W.W.) 2 Lanzhou University High School, Lanzhou 730000, China; [email protected] * Correspondences: [email protected] (Y.L.); [email protected] (K.G.); Tel.: +86-931-8912500 (Y.L.) Academic Editor: David Barker Received: 09 November 2018; Accepted: 29 November 2018; Published: 30 November 2018 Abstract: Lauraceae is a good source of lignans and neolignans, which are the most chemotaxonomic characteristics of many species of the family. This review describes 270 naturally occurring lignans and neolignans isolated from Lauraceae. Keywords: lignans; neolignans; Lauraceae; chemical components; chemical structures 1. Introduction Lignans are widely distributed in the plant kingdom, and show diverse pharmacological properties and a great number of structural possibilities. The Lauraceae family, especially the genera of Machilus, Ocotea, and Nectandra, is a rich source of lignans and neolignans, and neolignans represent potential chemotaxonomic significance in the study of the Lauraceae. Lignans and neolignans are dimers of phenylpropane, and conventionally classified into three classes: lignans, neolignans, and oxyneolignans, based on the character of the C–C bond and oxygen bridge joining the two typical phenyl propane units that make up their general structures [1]. Usually, lignans show dimeric structures formed by a β,β’-linkage (8,8’-linkage) between two phenylpropanes units. Meanwhile, the two phenylpropanes units are connected through a carbon–carbon bond, except for the 8,8’-linkage, which gives rise to neolignans. -
Supplementary Materials Evodiamine Inhibits Both Stem Cell and Non-Stem
Supplementary materials Evodiamine inhibits both stem cell and non-stem-cell populations in human cancer cells by targeting heat shock protein 70 Seung Yeob Hyun, Huong Thuy Le, Hye-Young Min, Honglan Pei, Yijae Lim, Injae Song, Yen T. K. Nguyen, Suckchang Hong, Byung Woo Han, Ho-Young Lee - 1 - Table S1. Short tandem repeat (STR) DNA profiles for human cancer cell lines used in this study. MDA-MB-231 Marker H1299 H460 A549 HCT116 (MDA231) Amelogenin XX XY XY XX XX D8S1179 10, 13 12 13, 14 10, 14, 15 13 D21S11 32.2 30 29 29, 30 30, 33.2 D7S820 10 9, 12 8, 11 11, 12 8 CSF1PO 12 11, 12 10, 12 7, 10 12, 13 D3S1358 17 15, 18 16 12, 16, 17 16 TH01 6, 9.3 9.3 8, 9.3 8, 9 7, 9.3 D13S317 12 13 11 10, 12 13 D16S539 12, 13 9 11, 12 11, 13 12 D2S1338 23, 24 17, 25 24 16 21 D19S433 14 14 13 11, 12 11, 14 vWA 16, 18 17 14 17, 22 15 TPOX 8 8 8, 11 8, 9 8, 9 D18S51 16 13, 15 14, 17 15, 17 11, 16 D5S818 11 9, 10 11 10, 11 12 FGA 20 21, 23 23 18, 23 22, 23 - 2 - Table S2. Antibodies used in this study. Catalogue Target Vendor Clone Dilution ratio Application1) Number 1:1000 (WB) ADI-SPA- 1:50 (IHC) HSP70 Enzo C92F3A-5 WB, IHC, IF, IP 810-F 1:50 (IF) 1 :1000 (IP) ADI-SPA- HSP90 Enzo 9D2 1:1000 WB 840-F 1:1000 (WB) Oct4 Abcam ab19857 WB, IF 1:100 (IF) Nanog Cell Signaling 4903S D73G4 1:1000 WB Sox2 Abcam ab97959 1:1000 WB ADI-SRA- Hop Enzo DS14F5 1:1000 WB 1500-F HIF-1α BD 610958 54/HIF-1α 1:1000 WB pAkt (S473) Cell Signaling 4060S D9E 1:1000 WB Akt Cell Signaling 9272S 1:1000 WB pMEK Cell Signaling 9121S 1:1000 WB (S217/221) MEK Cell Signaling 9122S 1:1000 -
Phytoestrogens : Daidzein, Enterodiol, Enterolactone, Equol, Geinstein, O-Desmethylangolensin Matrix: Urine Method: HPLC-ESI-MS/MS Method No: 4069.03
Laboratory Procedure Manual Analyte: Phytoestrogens : Daidzein, Enterodiol, Enterolactone, Equol, Geinstein, O-Desmethylangolensin Matrix: Urine Method: HPLC-ESI-MS/MS Method No: 4069.03 Revised: March 2018 as performed by: Nutritional Biomarkers Branch (NBB) Division of Laboratory Sciences (DLS) National Center for Environmental Health (NCEH) contact: James L. Pirkle, M.D., Ph.D. Director, Division of Laboratory Sciences Important Information for Users CDC periodically refines these laboratory methods. It is the responsibility of the user to contact the person listed on the title page of each write-up before using the analytical method to find out whether any changes have been made and what revisions, if any, have been incorporated. Phytoestrogen NHANES 2013-2014 This document details the Lab Protocol for testing the items listed in the following table. This method file describes measurements of U1PHYTO_H_R and U2PHYTO_H_R. One method was used to measure both the 24 hour urine phytoestrogen, 1st urine collection and 24 hour urine phytoestrogen, 2nd urine collection. However, these results are released as 2 separate data files. Variable File Name SAS Label (and SI units) Name Daidzein, Urine 1st collection UR1DAZ (ng/mL) o-Desmethylangolensin, Urine 1st UR1DMA Collection (ng/mL) UR1EQU Equol, Urine 1st Collection (ng/mL) Enterodiol, Urine 1st Collection UR1ETD (ng/mL) Enterolactone, Urine 1st Collection UR1ETL (ng/mL) Genistein, Urine 1st Collection UR1GNS (ng/mL) U1PT_H_R Daidzein, Urine 2nd collection U2PT_H_R UR2DAZ (ng/mL) o-Desmethylangolensin, Urine 2nd UR2DMA Collection (ng/mL) UR2EQU Equol, Urine 2nd Collection (ng/mL) Enterodiol, Urine 2nd Collection UR2ETD (ng/mL) Enterolactone, Urine 2nd Collection UR2ETL (ng/mL) Genistein, Urine 2nd Collection UR2GNS (ng/mL) 2 of 54 Phytoestrogen NHANES 2013-2014 1. -
Medicinal Properties of Selected Asparagus Species: a Review Polo-Ma-Abiele Hildah Mfengwana and Samson Sitheni Mashele
Chapter Medicinal Properties of Selected Asparagus Species: A Review Polo-Ma-Abiele Hildah Mfengwana and Samson Sitheni Mashele Abstract Asparagus species are naturally distributed along Asia, Africa, and Europe and are known to have numerous biological properties. This review article was aimed to provide an organized summary of current studies on the traditional uses, phy- tochemistry, and pharmacological and toxicological studies of Asparagus laricinus Burch., Asparagus africanus Lam., Asparagus officinalis L., Asparagus racemosus Willd., and Asparagus densiflorus (Kunth) Jessop to attain and establish new insights for further researches. Information used in this review was obtained from electronic database including PubMed central, Google scholars, Science direct, Scopus, and Sabinet. Based on the present findings, the existing literature still presents some breaches about the mechanism of action of various constituents of these plants, and their relation to other plant compounds in poly-herbal formulations, as well as their long-term use and safety. More in-depth studies are still needed for active compounds and biological activities of Asparagus laricinus, Asparagus africanus, and Asparagus densiflorus. Therefore, innumerable opportunities and possibilities for investigation are still available in novel areas of these plants for future research stud¬ies. It can be concluded that all selected Asparagus species have tremendous potential to improve human health and the pharmacological activities of these plants can be attributed to bioactive phytochemicals they possess. Keywords: Asparagaceae, Asparagus africanus lam., Asparagus densiflorus (kunth) Jessop, Asparagus laricinus Burch., Asparagus officinalis L., Asparagus racemosus Willd., pharmacological actions, phytochemistry 1. Introduction Historically, plants were used for numerous purposes for mankind in general, inter alia, feeding and catering, culinary spices, medicine, various forms of cosmetics, symbols in worship and for a variety of ornamental goods. -
7-Hydroxymatairesinol Improves Body Weight, Fat and Sugar Metabolism in C57BJ/6 Mice on a High-Fat Diet
Downloaded from British Journal of Nutrition (2018), 120, 751–762 doi:10.1017/S0007114518001824 © The Authors 2018 https://www.cambridge.org/core 7-Hydroxymatairesinol improves body weight, fat and sugar metabolism in C57BJ/6 mice on a high-fat diet Giorgio Biasiotto1,2†, Isabella Zanella1,2†, Federica Predolini1,2, Ivonne Archetti3, Moris Cadei4, . IP address: Eugenio Monti2, Marcello Luzzani5, Barbara Pacchetti5, Paola Mozzoni6, Roberta Andreoli6, Giuseppe De Palma7, Federico Serana1, Annika Smeds8 and Diego Di Lorenzo1* 170.106.34.90 1Clinical Chemistry Laboratory, Diagnostic Department, ASST Spedali Civili di Brescia, P. Le Spedali Civili 1, 25123 Brescia, Italy 2Department of Molecular and Translational Medicine, University of Brescia, Via Valsabbina 1, 25123 Brescia, Italy , on 3Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER), “Bruno Ubertini”, Via Bianchi, 9, 02 Oct 2021 at 07:24:15 25124 Brescia, Italy 4Human Pathology, School of Medicine, University of Brescia, P. Le Spedali Civili 1, 25123 Brescia, Italy 5Linnea SA, via Cantonale 123, CH-6595 Riazzino, Switzerland 6Laboratory of Industrial Toxicology, Department of Medicine and Surgery, University of Parma, 43126 Parma, Italy 7Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, Section of Public Health and Human Sciences, University of Brescia, P. Le Spedali Civili 1, 25123 Brescia, Italy , subject to the Cambridge Core terms of use, available at 8Laboratory of Wood and Paper Chemistry, Åbo Akademi University, 20500 Turku, Finland (Submitted 18 December 2017 – Final revision received 30 April 2018 – Accepted 15 May 2018 – First published online 14 August 2018) Abstract 7-Hydroxymatairesinol (7-HMR) is a plant lignan abundant in various concentrations in plant foods.