Single Laboratory Validation of a Quantitative Core Shell-Based LC
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Pinoresinol Reductase 1 Impacts Lignin Distribution During Secondary Cell Wall Biosynthesis in Arabidopsis
Phytochemistry xxx (2014) xxx–xxx Contents lists available at ScienceDirect Phytochemistry journal homepage: www.elsevier.com/locate/phytochem Pinoresinol reductase 1 impacts lignin distribution during secondary cell wall biosynthesis in Arabidopsis Qiao Zhao a, Yining Zeng b,e, Yanbin Yin c, Yunqiao Pu d,e, Lisa A. Jackson a,e, Nancy L. Engle e,f, Madhavi Z. Martin e,f, Timothy J. Tschaplinski e,f, Shi-You Ding b,e, Arthur J. Ragauskas d,e, ⇑ Richard A. Dixon a,e,g, a Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA b Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA c Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA d Institute of Paper Science and Technology, Georgia Institute of Technology, Atlanta, GA, USA e BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA f Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA g Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA article info abstract Article history: Pinoresinol reductase (PrR) catalyzes the conversion of the lignan (À)-pinoresinol to (À)-lariciresinol in Available online xxxx Arabidopsis thaliana, where it is encoded by two genes, PrR1 and PrR2, that appear to act redundantly. PrR1 is highly expressed in lignified inflorescence stem tissue, whereas PrR2 expression is barely detect- Keywords: able in stems. Co-expression analysis has indicated that PrR1 is co-expressed with many characterized Lignan genes involved in secondary cell wall biosynthesis, whereas PrR2 expression clusters with a different Lignin set of genes. -
Novel Bioactive Extraction and Nano-Encapsulation
Entry Novel Bioactive Extraction and Nano-Encapsulation Shaba Noore 1,2 , Navin Kumar Rastogi 3, Colm O’Donnell 2 and Brijesh Tiwari 1,2,* 1 Department of Food Chemistry & Technology, Teagasc Food Research Centre, Ashtown, D15 DY05 Dublin, Ireland; [email protected] 2 School of Biosystems and Food Engineering, University College Dublin, Belfield, D04 V1W8 Dublin, Ireland; [email protected] 3 Department of Food Engineering, CSIR-Central Food Technological Research Institute, Mysuru 570020, India; [email protected] * Correspondence: [email protected] Definition: An extraction technology works on the principle of two consecutive steps that involves mixture of solute with solvent and the movement of soluble compounds from the cell into the solvent and its consequent diffusion and extraction. The conventional extraction techniques are mostly based on the use of mild/high temperatures (50–90 ◦C) that can cause thermal degradation, are dependent on the mass transfer rate, being reflected on long extraction times, high costs, low extraction efficiency, with consequent low extraction yields. Due to these disadvantages, it is of interest to develop non-thermal extraction methods, such as microwave, ultrasounds, supercritical fluids (mostly using carbon dioxide, SC-CO2), and high hydrostatic pressure-assisted extractions which works on the phenomena of minimum heat exposure with reduced processing time, thereby minimizing the loss of bioactive compounds during extraction. Further, to improve the stability of these extracted compounds, nano-encapsulation is required. Nano-encapsulation is a process which forms a thin layer of protection against environmental degradation and retains the nutritional and Citation: Noore, S.; Rastogi, N.K.; functional qualities of bioactive compounds in nano-scale level capsules by employing fats, starches, O’Donnell, C.; Tiwari, B. -
Silychristin Derivatives Conjugated with Coniferylalcohols from Silymarin and Their Pancreatic Α-Amylase Inhibitory Title Activity
Silychristin derivatives conjugated with coniferylalcohols from silymarin and their pancreatic α-amylase inhibitory Title activity Author(s) Kato, Eisuke; Kushibiki, Natsuka; Satoh, Hiroshi; Kawabata, Jun Natural Product Research, 34(6), 759-765 Citation https://doi.org/10.1080/14786419.2018.1499639 Issue Date 2020-03-18 Doc URL http://hdl.handle.net/2115/80605 This is an Accepted Manuscript of an article published by Taylor & Francis in Natural Product Research on Rights Mar.18.2020, available online: http://www.tandfonline.com/10.1080/14786419.2018.1499639. Type article (author version) File Information EK_NatProdRes_milk_thistle_w_supplement.pdf Instructions for use Hokkaido University Collection of Scholarly and Academic Papers : HUSCAP *Post-print manuscript This document is the unedited Author's version of a Submitted Work that was subsequently accepted for publication in “Natural Product Research” published by Taylor & Francis after peer review. To access the final edited and published work see https://doi.org/10.1080/14786419.2018.1499639 Graphical abstract 1 RESEARCH ARTICLE Silychristin derivatives conjugated with coniferylalcohols from silymarin and their pancreatic α-amylase inhibitory activity Eisuke Katoa*, Natsuka Kushibikib, Hiroshi Satohc and Jun Kawabataa aDivision of Fundamental AgriScience and Research, Research Faculty of Agriculture, Hokkaido University, Kita-ku, Sapporo, Hokkaido 060-8589, Japan bDivision of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo, Hokkaido 060-8589, Japan cNissei Bio Co. Ltd., Eniwa, Hokkaido, 061-1374, Japan *Corresponding author. Eisuke Kato, 1Division of Fundamental AgriScience and Research, Research Faculty of Agriculture, Hokkaido University, Kita-ku, Sapporo, Hokkaido 060-8589, Japan; Tel/Fax: +81 11 706 2496; e-mail: [email protected] 2 Abstract Silymarin is a mixture of flavonolignans extracted from the fruit of Silybum marianum (milk thistle). -
Total Phenolic Content, Free Radical Scavenging Capacity, and Anti- Cancer Activity of Silymarin
Journal of International Society for Food Bioactives Nutraceuticals and Functional Foods Review J. Food Bioact. 2020;10:53–63 Total phenolic content, free radical scavenging capacity, and anti- cancer activity of silymarin Uyory Choea, Monica Whenta*, Yinghua Luob and Liangli Yua aDepartment of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA bCollege of Food Science and Engineering, National Engineering Research Center for Fruit and Vegetable Processing, Ministry of Educa- tion, China Agricultural University, Beijing, China *Corresponding author: Monica Whent, Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA. Tel: +1 301 4054521; E-mail: [email protected] DOI: 10.31665/JFB.2020.10227 Received: June 19, 2020; Revised received & accepted: June 29, 2020 Citation: Choe, U., Whent, M., Luo, Y., and Yu, L. (2020). Total phenolic content, free radical scavenging capacity, and anti-cancer activity of silymarin. J. Food Bioact. 10: 53–63. Abstract Milk thistle (Silybum marianum) seeds are a good source of dietary polyphenols. The bioactive component of milk thistle seeds, silymarin, contains flavonolignans including silybin A, silybin B, isosilybin A, isosilybin B, sily- christin, isosilychristin, and silydiain along with the flavonol taxifolin. Silymarin is used traditionally as a natural herbal medicine with minimal side effects. Structurally, each silymarin component possesses phenolic hydroxyl groups and thus works as an antioxidant. In addition to free radical scavenging capacities, silymarin’s anti-cancer activities were reported for many different types of cancers including bladder, breast, colon, gastric, kidney, lung, oral, ovarian, prostate, and skin. The current review will discuss silymarin’s chemical components, total phenolic content, free radical scavenging capacities, and anti-cancer activities. -
Flavonoids: Potential Candidates for the Treatment of Neurodegenerative Disorders
biomedicines Review Flavonoids: Potential Candidates for the Treatment of Neurodegenerative Disorders Shweta Devi 1,†, Vijay Kumar 2,*,† , Sandeep Kumar Singh 3,†, Ashish Kant Dubey 4 and Jong-Joo Kim 2,* 1 Systems Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research, Lucknow 226001, India; [email protected] 2 Department of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 38541, Korea 3 Department of Medical Genetics, SGPGIMS, Lucknow 226014, India; [email protected] 4 Department of Neurology, SGPGIMS, Lucknow 226014, India; [email protected] * Correspondence: [email protected] (V.K.); [email protected] (J.-J.K.); Tel.: +82-10-9668-3464 (J.-J.K.); Fax: +82-53-801-3464 (J.-J.K.) † These authors contributed equally to this work. Abstract: Neurodegenerative disorders, such as Parkinson’s disease (PD), Alzheimer’s disease (AD), Amyotrophic lateral sclerosis (ALS), and Huntington’s disease (HD), are the most concerning disor- ders due to the lack of effective therapy and dramatic rise in affected cases. Although these disorders have diverse clinical manifestations, they all share a common cellular stress response. These cellular stress responses including neuroinflammation, oxidative stress, proteotoxicity, and endoplasmic reticulum (ER)-stress, which combats with stress conditions. Environmental stress/toxicity weakened the cellular stress response which results in cell damage. Small molecules, such as flavonoids, could reduce cellular stress and have gained much attention in recent years. Evidence has shown the poten- tial use of flavonoids in several ways, such as antioxidants, anti-inflammatory, and anti-apoptotic, yet their mechanism is still elusive. This review provides an insight into the potential role of flavonoids against cellular stress response that prevent the pathogenesis of neurodegenerative disorders. -
Expanding the Coverage of the Metabolic Landscape in Cultivated Rice with Integrated Computational Approaches
bioRxiv preprint doi: https://doi.org/10.1101/2020.03.04.976266; this version posted March 5, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Expanding the Coverage of the Metabolic Landscape in 2 Cultivated Rice with Integrated Computational Approaches 3 4 5 Xuetong Li1,4,#,a, Hongxia Zhou1,4,#,b, Ning Xiao2,c, Xueting Wu1,d, Yuanhong Shan1,e, 6 Longxian Chen1, 4,f, Cuiting Wang1,g, Zixuan Wang1,h, Jirong Huang3,*,i, Aihong Li2,*,j, 7 and Xuan Li1,4,*,k 8 9 1Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant 10 Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 11 Shanghai 200032, China 12 2Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou 225007, 13 China 14 3Department of Biology, College of Life and Environmental Sciences, Shanghai 15 Normal University, Shanghai 200234, China 16 4University of Chinese Academy of Sciences, Beijing 100039, China 17 18 19 20 # Equal contribution. 21 * Corresponding authors. 22 Email: [email protected] (Li X), [email protected] (Li A), [email protected] 23 (Huang J) 24 25 26 Running title: Li X et al / Expanding the Coverage of the Metabolic Landscape 1 / 32 bioRxiv preprint doi: https://doi.org/10.1101/2020.03.04.976266; this version posted March 5, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. -
1.25 Lignans: Biosynthesis and Function
1.25 Lignans: Biosynthesis and Function NORMAN G. LEWIS and LAURENCE B. DAVIN Washington State University, Pullman, WA, USA 0[14[0 INTRODUCTION 539 0[14[1 DEFINITION AND NOMENCLATURE 539 0[14[2 EVOLUTION OF THE LIGNAN PATHWAY 531 0[14[3 OCCURRENCE 534 0[14[3[0 Li`nans in {{Early|| Land Plants 534 0[14[3[1 Li`nans in Gymnosperms and An`iosperms "General Features# 536 0[14[4 OPTICAL ACTIVITY OF LIGNAN SKELETAL TYPES AND LIMITATIONS TO THE FREE RADICAL RANDOM COUPLING HYPOTHESIS 536 0[14[5 707? STEREOSELECTIVE COUPLING] DIRIGENT PROTEINS AND E!CONIFERYL ALCOHOL RADICALS 541 0[14[5[0 Diri`ent Proteins Stipulate Stereoselective Outcome of E!Coniferyl Alcohol Radical Couplin` in Pinoresinol Formation 541 0[14[5[1 Clonin` of the Gene Encodin` the Diri`ent Protein and Recombinant Protein Expression in Heterolo`ous Systems 543 0[14[5[2 Sequence Homolo`y Comparisons 543 0[14[5[3 Comparable Systems 543 0[14[5[4 Perceived Biochemical Mechanism of Action 546 0[14[6 PINORESINOL METABOLISM AND ASSOCIATED METABOLIC PROCESSES 547 0[14[6[0 Sesamum indicum] "¦#!Piperitol\ "¦#!Sesamin\ and "¦#!Sesamolinol Synthases 547 0[14[6[1 Magnolia kobus] Pinoresinol and Pinoresinol Monomethyl Ether O!Methyltransferase"s# 550 0[14[6[2 Forsythia intermedia and Forsythia suspensa 551 0[14[6[2[0 "¦#!Pinoresinol:"¦#!lariciresinol reductase 552 0[14[6[2[1 "−#!Secoisolariciresinol dehydro`enase 554 0[14[6[2[2 Matairesinol O!methyltransferase 556 0[14[6[3 Linum usitatissimum] "−#!Pinoresinol:"−#!Lariciresinol Reductase and "¦#!Secoisolariciresinol Glucosyltransferase"s# 557 -
Addis Ababa University College of Health Sciences
ADDIS ABABA UNIVERSITY COLLEGE OF HEALTH SCIENCES SCHOOL OF MEDICINE DEPARTMENT OF BIOCHEMISTRY Hepatoprotective Effect of Silymarin on Fructose Induced Nonalcoholic Fatty Liver Disease in Male Albino Wistar Rats. By: Tewodros Mengesha (BSc.) A Thesis Submitted To School of Graduate Studies, College of Health Sciences, Addis Ababa University In Partial Fulfillment of The Requirement For The Degree of Master of Sciences In Medical Biochemistry. June, 2016 Addis Ababa, Ethiopia Hepatoprotective Effect of Silymarin on Fructose Induced Nonalcoholic Fatty Liver Disease in Male Albino Wistar Rats. Advisors 1. N. Gnana Sekeheram, PhD Department of Biochemistry, School of Medicine Addis Ababa University, Addis Ababa, Ethiopia. 2. Mahilet Arayasilasie, MD, Pathologist Head, Department of Pathology, School of Medicine Addis Ababa University, Addis Ababa, Ethiopia. Addis Ababa University School of Graduate Studies This is to clarify that thesis prepared by Tewodros Mengesha entitled “Hepatoprotective Effect of Silymarin on Fructose Induced Nonalcoholic Fatty Liver Disease in Male Albino Wistar Rats” is submitted in partial fulfillment of the Requirement for the Degree of Master of Sciences in Medical Biochemistry complies with the regulations of the university and meets the accepted standards with respect to the originality and quality. Signed by the examining committee: Examiner Signature Date Advisor Signature Date Advisor Signature Date Advisor Signature Date Chair of Department or Graduate Program Coordinator Abstract Background: Nonalcoholic fatty liver disease is one of the most common causes of chronic liver disease in the Western world, and it‟s likely to parallel the increasing prevalence of type 2 diabetes, obesity, and other components of metabolic syndrome. There is also growing evidence in both animal models and human studies suggesting that high dietary intake of fructose is an important nutritional factor in the development of metabolic syndrome and its associated complications of NAFLD. -
Bioconversion of Callus-Produced Precursors to Silymarin Derivatives in Silybum Marianum Leaves for the Production of Bioactive Compounds
International Journal of Molecular Sciences Article Bioconversion of Callus-Produced Precursors to Silymarin Derivatives in Silybum marianum Leaves for the Production of Bioactive Compounds Dina Gad 1,2,* , Hamed El-Shora 3, Daniele Fraternale 4 , Elisa Maricchiolo 4, Andrea Pompa 4,* and Karl-Josef Dietz 2 1 Botany and Microbiology Department, Faculty of Science, Menoufia University, Shebin EL-Koum 32511, Egypt 2 Biochemistry and Physiology of Plants, Faculty of Biology W5, Bielefeld University, 33501 Bielefeld, Germany; [email protected] 3 Botany Department, Faculty of Science, Mansoura University, Mansoura 35511, Egypt; [email protected] 4 Department of Biomolecular Sciences, University of Urbino “Carlo Bo” Via Donato Bramante, 28, 61029 Urbino, Italy; [email protected] (D.F.); [email protected] (E.M.) * Correspondence: [email protected]fia.edu.eg (D.G.); [email protected] (A.P.) Abstract: The present study aimed to investigate the enzymatic potential of Silybum marianum leaves to bioconvert phenolic acids produced in S. marianum callus into silymarin derivatives as chemopreventive agent. Here we demonstrate that despite the fact that leaves of S. marianum did not accumulate silymarin themselves, expanding leaves had the full capacity to convert di- caffeoylquinic acid to silymarin complex. This was proven by HPLC separations coupled with Citation: Gad, D.; El-Shora, H.; electrospray ionization mass spectrometry (ESI-MS) analysis. Soaking the leaf discs with S. marianum Fraternale, D.; Maricchiolo, E.; callus extract for different times revealed that silymarin derivatives had been formed at high yield Pompa, A.; Dietz, K.-J. Bioconversion after 16 h. Bioconverted products displayed the same retention time and the same mass spectra (MS of Callus-Produced Precursors to or MS/MS) as standard silymarin. -
Lung Cancer Management with Silibinin: a Historical and Translational Perspective
pharmaceuticals Review Lung Cancer Management with Silibinin: A Historical and Translational Perspective Sara Verdura 1,2,† , Elisabet Cuyàs 1,2,†, Verónica Ruiz-Torres 3 , Vicente Micol 3 , Jorge Joven 4 , Joaquim Bosch-Barrera 2,5,6,* and Javier A. Menendez 1,2,* 1 Girona Biomedical Research Institute (IDIBGI), 17190 Girona, Spain; [email protected] (S.V.); [email protected] (E.C.) 2 Metabolism and Cancer Group, Program against Cancer Therapeutic Resistance (ProCURE), Catalan Institute of Oncology, 17007 Girona, Spain 3 Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE) and Instituto de Biología Molecular y Celular (IBMC), Universidad Miguel Hernández (UMH), 03202 Elche, Spain; [email protected] (V.R.-T.); [email protected] (V.M.) 4 Unitat de Recerca Biomèdica (URB-CRB), Hospital Universitari de Sant Joan, Institut d’Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, 43201 Reus, Spain; [email protected] 5 Medical Oncology, Catalan Institute of Oncology, Dr. Josep Trueta Hospital of Girona, 17007 Girona, Spain 6 Department of Medical Sciences, Faculty of Medicine, University of Girona (UdG), 17003 Girona, Spain * Correspondence: [email protected] (J.B.-B.); [email protected] (J.A.M.) † Both authors contributed equally to this work. Abstract: The flavonolignan silibinin, the major bioactive component of the silymarin extract of Silybum marianum (milk thistle) seeds, is gaining traction as a novel anti-cancer therapeutic. Here, we review the historical developments that have laid the groundwork for the evaluation of silibinin as a chemopreventive and therapeutic agent in human lung cancer, including translational insights Citation: Verdura, S.; Cuyàs, E.; into its mechanism of action to control the aggressive behavior of lung carcinoma subtypes prone Ruiz-Torres, V.; Micol, V.; Joven, J.; to metastasis. -
Skin Aging Handbook
SKIN AGING HANDBOOK An Integrated Approach to Biochemistry and Product Development Edited by Nava Dayan Norwich, NY, USA Copyright © 2008 by William Andrew Inc. No part of this book may be reproduced or utilized in any form or by any means, electronic or me- chanical, including photocopying, recording, or by any information storage and retrieval system, without permission in writing from the Publisher. ISBN: 978-0-8155-1584-5 Library of Congress Cataloging-in-Publication Data Skin aging handbook : an integrated approach to biochemistry and product development / edited by Nava Dayan. p. ; cm. -- (Personal care and cosmetic technology) Includes bibliographical references and index. ISBN 978-0-8155-1584-5 (alk. paper) 1. Skin--Aging. 2. Cosmetics. 3. Dermatologic agents. 4. Cosmetic industry. 5. Dermatologic agents industry. I. Dayan, Nava. II. Series. [DNLM: 1. Skin Physiology--drug effects. 2. Aging--drug effects. 3. Chemistry, Pharmaceutical. 4. Cosmetics--economics. 5. Cosmetics--pharmacology. 6. Cosmetics--therapeutic use. WR 102 S62715 2008] QP88.5.S553 2008 612.7’9--dc22 2008009757 Printed in the United States of America This book is printed on acid-free paper. 10 9 8 7 6 5 4 3 2 1 Published by: William Andrew Inc. 13 Eaton Avenue Norwich, NY 13815 1-800-932-7045 www.williamandrew.com Cover Design by Russell Richardson ENVIRONMENTALLY FRIENDLY This book has been printed digitally because this process does not use any plates, ink, chemicals, or press solutions that are harmful to the environment. The paper used in this book has a 30% recycled content. NOTICE To the best of our knowledge the information in this publication is accurate; however the Publisher does not assume any responsibility or liability for the accuracy or completeness of, or consequences arising from, such information. -
Evaluation of the Anti-Cancer Potential of Cedrus Deodara Total Lignans By
Shi et al. BMC Complementary and Alternative Medicine (2019) 19:281 https://doi.org/10.1186/s12906-019-2682-6 RESEARCH ARTICLE Open Access Evaluation of the anti-cancer potential of Cedrus deodara total lignans by inducing apoptosis of A549 cells Xiaofeng Shi1,2*, Ruiqin Du1, Junmin Zhang3, Yanping Lei2 and Hongyun Guo2 Abstract Background: Cedrus deodara (Roxb.) Loud (normally called as deodar), one out of four species in the genus Cedrus, exhibits widely biological activities. The Cedrus deodara total lignans from the pine needles (CTL) were extracted. The aim of the study was to investigate the anticancer potential of the CTL on A549 cell line. Methods: We extracted the CTL by ethanol and assessed the cytotoxicity by CCK-8 method. Cell cycle and apoptosis were detected by a FACS Verse Calibur flow cytometry. Results: The CTL were extracted by means of ethanol hot refluxing and the content of total lignans in CTL was about 55.77%. By the CCK-8 assays, CTL inhibited the growth of A549 cells in a dose-dependent fashion, with the IC50 values of 39.82 ± 1.74 μg/mL. CTL also inhibited the growth to a less extent in HeLa, HepG2, MKN28 and HT-29 cells. Conclusion: At low doses, the CTL effectively inhibited the growth of A549 cells. By comparison of IC50 values, we found that A549 cells might be more sensitive to the treatment with CTL. In addition, CTL were also able to increase the population of A549 cells in G2/M phase and the percentage of apoptotic A549 cells. CTL may have therapeutic potential in lung adenocarcinoma cancer by regulating cell cycle and apoptosis.