Regulation of 3D Chromatin Organization by CTCF
Total Page：16
File Type：pdf, Size：1020Kb
Available online at www.sciencedirect.com ScienceDirect Regulation of 3D chromatin organization by CTCF Jian-Feng Xiang and Victor G Corces Studies of nuclear architecture using chromosome multivalent proteins [1]. The use of high throughput chro- conformation capture methods have provided a detailed view mosome conformation capture methods has resulted in a of how chromatin folds in the 3D nuclear space. New variants of more detailed view of chromatin 3D organization. Based on this technology now afford unprecedented resolution and allow the original results in which the resolution of the Hi-C data the identification of ever smaller folding domains that offer new ranged between 1 Mb and 50 kb, the general consensus in insights into the mechanisms by which this organization is the field suggests that mammalian chromosomes are orga- established and maintained. Here we review recent results in nized into large >1 Mb compartments, identified by PC1 this rapidly evolving field with an emphasis on CTCF function, after Principal Component Analysis (PCA), that can be with the goal of gaining a mechanistic understanding of the classified into two types, A and B, which respectively principles by which chromatin is folded in the eukaryotic correlate with active or inactive chromatin [2]. In addition nucleus. to large compartments, chromosomes contain smaller Topologically Associating Domains (TADs) that can be Address identified using algorithms that detect changes in the Emory University School of Medicine, Department of Human Genetics, directionality of interactions. A subset of TADs contains 615 Michael Street, Atlanta, GA 30322, USA CTCF at the borders and correspond to CTCF loops whereas others are flanked by actively transcribed genes Corresponding author: Corces, Victor G ([email protected], [email protected]) with or without CTCF [3]. Here we use the term loop to refer to a domain created by relatively stable point to point contacts, such as those mediated by CTCF/cohesin, and Current Opinion in Genetics and Development 2021, 67:33–40 visible in Hi-C heatmaps as strong punctate signal at the This review comes from a themed issue on Genome architecture and summit of the domain. Loops are different from other expression contact domains formed by interactions among sequences Edited by Susan Gasser and Gerd Blobel within the domain mediated by proteins present in these sequences and corresponding to their transcriptional state. In this review we first consider the distinct contributions of transcriptional state and CTCF/cohesin to 3D chromatin https://doi.org/10.1016/j.gde.2020.10.005 organization. We then focus on mechanisms by which 0959-437X/ã 2020 Elsevier Ltd. All rights reserved. CTCF function is regulated to promote the formation of stable loops that can modulate enhancer–promoter communication. 3D nuclear organization, transcription, and CTCF loops Introduction Results from high resolution Hi-C, around 1 kb for mam- The eukaryotic genome is organized in the three- malian cells and 250 bp for Drosophila, suggest that eukary- dimensional nuclear space in a manner that responds to otic genomes are organized into small contact domains and facilitates the regulation of nuclear processes. This containing one or several closely spaced genes in the same organization therefore affects, and may be affected by, transcriptional state. Since these contact domains can be critical nuclear functions such as transcription, replication, identified by PCA using bin sizes of 5À10 kb, they have recombination, and DNA repair. Until recently, insights been called compartment(al) domains to highlight their into the 3D organization of the genetic material have come similarity to the original >1 Mb compartments [4,5]. Inter- mostly from the use of microscopy, which has given us a actions among compartmental domains give rise to the broad view of nuclear architecture. Results from these plaid pattern observed in Hi-C heatmaps. This interaction studies suggest that actively transcribed regions interact pattern explains the formation of membraneless organelles to form various types of membraneless organelles such as or biomolecular condensates observed by microscopy and transcription factories, regions of the genome containing biochemical studies. Recent analyses of chromatin archi- H3K27me3 and Polycomb (Pc) complexes PRC1 and tecture using variations of the original Hi-C method have PRC2 congregate at Pc bodies, and regions containing shed further light into the existence of small contact H3K9me3 and HP1 such as centromeres come together domains that correlate with transcriptional state and may to form chromocenters. Biochemical analyses of proteins represent the basic unit of chromosome organization. involved in these contacts suggest that these structures Micro-C employs micrococcal nuclease, rather than restric- are biomolecular condens formed by interactions among tion enzymes, to digest the chromatin, thus allowing a www.sciencedirect.com Current Opinion in Genetics & Development 2021, 67:33–40 34 Genome architecture and expression uniform nucleosome-size length of the digested fragments domains alternate with non-transcribed sequences, which [6,7]. Micro-C allows the visualization of compartmental may contain H3K27me3, H3K9me3, or neither, and form domains composed of single genes, including enhancer– self-interacting compartmental domains. Each of these promoter interactions, and involved in long-range interac- types of domains interact with other domains in the same tions with other compartmental domains. When expressed, transcriptional state to give rise to the plaid pattern these domains are disrupted by inhibition of transcription, observed in Hi-C heatmaps away from the diagonal. These suggesting that they arise as a consequence of interactions long-range interactions are heterogeneous in different cells among proteins involved in the transcription process [6,7], of a population. Therefore, this organization is a conse- probably including initiation, splicing and termination. quence of the transcriptional state of sequences that is, an This may explain why simply depleting the different emergent property of the one-dimensional epigenetic RNA polymerases has little effect on chromatin 3D orga- information present in the chromatin fiber [12]. However, nization [8], sinceit is possiblethat other components of the once this organization is established, it can affect gene transcription machinery or proteins associated with cova- expression by increasing the concentration of various fac- lent histone modifications may still remain on chromatin, tors in biomolecular condensates established in distinct although this has not been confirmed experimentally. nuclear compartments. Small contact domains that correlate with transcriptional state have also been observed using CAP-C, a derivative of The regulation of the transcriptional state-dependent 3D Hi-C that uses multifunctional chemical crosslinkers to organization of the genome takes place by well-estab- capture long-range chromatin interactions [9]. CAP-C lished biochemical principles determined by the affinities achieves much higher resolution, allowing the identifica- of transcription factors for specific DNA sequences and tion of short-range interactions, which are present close to for other proteins, which allow the recruitment of protein thediagonal ofHi-Cheatmapsandnotresolvedbystandard complexes to chromatin. Superimposed on this transcrip- Hi-C. Using CAP-C, it has been possible to characterize tional state-dependent aspect of nuclear architecture is loop domains that is, contact domains with strong punctate the phenomenon of cohesin extrusion, which takes place signal at the summit and identifiable by tools such as throughout the genome and can be stopped by CTCF. HiCCUPS. Loop domainsareformed by cohesin extrusion, Interactions within and between compartmental domains have convergent CTCF sites at their anchors, and are are disrupted by CTCF and cohesin mediated loop affected by CTCF depletion. Nonloop domains contain extrusion [12]. The cohesin complex is loaded throughout single genes,rangeinsize between 10 kb and40 kb, andare the genome, or perhaps preferentially at NIPBL sites. affected by transcription inhibition when present in the A Cohesin extrusion may be slowed down at genomic sites compartment [9]. The independent contribution of CTCF containing specific proteins or large protein complexes and transcription to 3D chromatin organization has been [6,7]. However, cohesin extrusion is stopped by sites recently tested in a series of elegant experiments involving bound by the CTCF protein, preferentially when the either swapping the Xist/Tsix transcriptional units [10] or sites are arranged in a convergent orientation [13–15]. inserting a 2 kb sequence containing a CTCF site and a This retention of the cohesin complex at CTCF sites TSS [11]. The consequence of the presence of this 2 kb results in the formation of relatively stable loops that can fragment in different genomic locations was analyzed by be visualized in Hi-C heatmaps as strong puncta that Hi-C. Depending on the genomic context, the inserted represent interactions between two anchors containing CTCF site can make loops with adjacent sites in the CTCF. The strong punctate signal clearly visible above genome whereas the TSS creates new domains by forming the surrounding background in Hi-C heatmaps suggests directionalcontactswith downstreamsequences.When the that these loops may be sufficiently stable to be simulta- fragment is