What Is the Ebola Virus How Is Ebola Spread?
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Development, Manufacturing, and Supply of MSD's Ebola Vaccine
Engineering Conferences International ECI Digital Archives Vaccine Technology VI Proceedings 6-15-2016 Development, manufacturing, and supply of MSD’s Ebola vaccine Jeffrey T. Blue Director Vaccine Drug Product Development, Merck Sharp & Dohme Corp., USA, [email protected] Follow this and additional works at: http://dc.engconfintl.org/vaccine_vi Part of the Engineering Commons Recommended Citation Jeffrey T. Blue, "Development, manufacturing, and supply of MSD’s Ebola vaccine" in "Vaccine Technology VI", Laura Palomares, UNAM, Mexico Manon Cox, Protein Sciences Corporation, USA Tarit Mukhopadhyay, University College London, UK Nathalie Garçon, BIOASTER Technology Research Institute, FR Eds, ECI Symposium Series, (2016). http://dc.engconfintl.org/vaccine_vi/ 23 This Abstract and Presentation is brought to you for free and open access by the Proceedings at ECI Digital Archives. It has been accepted for inclusion in Vaccine Technology VI by an authorized administrator of ECI Digital Archives. For more information, please contact [email protected]. Development, Manufacturing, and Supply of MSD’s Ebola Vaccine Jeffrey T. Blue Director, Vaccine Drug Product Development Merck & Co. Inc. Vaccine Technology VI 15 June 2016 Albufeira, Portugal • Elements of this program has been funded in whole or in part with Federal Funds from the Department of Health and Human Services; Office of the Assistant Secretary for Preparedness and Response; Biomedical Advanced Research and Development Authority under Contract Number: HHSO100201500002C Presentation -
Signature Redacted Signature of Author
Developing VHH-based tools to study Ebolavirus Infection By Jason V. M. H. Nguyen B.S., Biochemistry and Molecular Biology University of California, Santa Cruz, 2012 Submitted to the Microbiology Graduate Program in partial fulfillment of the requirements for the degree of Doctor of Philosophy At the Massachusetts Institute of Technology June 2019 2019 Massachusetts Institute of Technology. All rights reserved. Signature redacted Signature of Author...... .................................................................... V Y' Jason V. M. H. Nguyen Microbiology Graduate Program May 13, 2019 Signature redacted Certified by.............. ................................................................... Hidde L. Ploegh Senior Investigator Boston Children's Hospital It Signature redacted Accepted by....... ......................................................................... Jacquin C. Niles Associate Professor of Biological Engineering Chair of Microbiology Program MASSACHUSETTS INSTITUTE OF TECHNOLOGY JUN 2 12019 1 LIBRARIES ARCHIVES 2 Developing VHH-based tools to study Ebolavirus Infection By Jason V. M. H. Nguyen Submitted to the Microbiology Graduate Program on May 13th, 2019 in partial fulfillment of the requirements for the degree of Doctor of Philosophy ABSTRACT Variable domains of camelid-derived heavy chain-only antibodies, or VHHs, have emerged as a unique antigen binding moiety that holds promise in its versatility and utilization as a tool to study biological questions. This thesis focuses on two aspects on developing tools to study infectious disease, specifically Ebolavirus entry. In Chapter 1, I provide an overview about antibodies and how antibodies have transformed the biomedical field and how single domain antibody fragments, or VHHs, have entered this arena. I will also touch upon how VHHs have been used in various fields and certain aspects that remain underexplored. Chapter 2 focuses on the utilization of VHHs to study Ebolavirus entry using VHHs that were isolated from alpacas. -
Democratic Republic of the Congo – Ebola Outbreaks SEPTEMBER 30, 2020
Fact Sheet #10 Fiscal Year (FY) 2020 Democratic Republic of the Congo – Ebola Outbreaks SEPTEMBER 30, 2020 SITUATION AT A GLANCE 128 53 13 3,470 2,287 Total Confirmed and Total EVD-Related Total EVD-Affected Total Confirmed and Total EVD-Related Probable EVD Cases in Deaths in Équateur Health Zones in Probable EVD Cases in Deaths in Eastern DRC Équateur Équateur Eastern DRC at End of at End of Outbreak Outbreak MoH – September 30, 2020 MoH – September 30, 2020 MoH – September 30, 2020 MoH – June 25, 2020 MoH – June 25, 2020 Health actors remain concerned about surveillance gaps in northwestern DRC’s Équateur Province. In recent weeks, several contacts of EVD patients have travelled undetected to neighboring RoC and the DRC’s Mai- Ndombe Province, heightening the risk of regional EVD spread. Logistics coordination in Equateur has significantly improved in recent weeks, with response actors establishing a Logistics Cluster in September. The 90-day enhanced surveillance period in eastern DRC ended on September 25. TOTAL USAID HUMANITARIAN FUNDING USAID/BHA1,2 $152,614,242 For the DRC Ebola Outbreaks Response in FY 2020 USAID/GH in $2,500,000 Neighboring Countries3 For complete funding breakdown with partners, see funding chart on page 6 Total $155,114,2424 1USAID’s Bureau for Humanitarian Assistance (USAID/BHA) 2 Total USAID/BHA funding includes non-food humanitarian assistance from the former Office of U.S. Foreign Disaster Assistance. 3 USAID’s Bureau for Global Health (USAID/GH) 4 Some of the USAID funding intended for Ebola virus disease (EVD)-related programs in eastern Democratic Republic of the Congo (DRC) is now supporting EVD response activities in Équateur. -
2019 Icar Program & Abstracts Book
Hosted by the International Society for Antiviral Research (ISAR) ND International Conference 32on Antiviral Research (ICAR) Baltimore MARYLAND PROGRAM and USA Hyatt Regency BALTIMORE ABSTRACTS May 12-15 2019 ND TABLE OF International Conference CONTENTS 32on Antiviral Research (ICAR) Daily Schedule . .3 Organization . 4 Contributors . 5 Keynotes & Networking . 6 Schedule at a Glance . 7 ISAR Awardees . 10 The 2019 Chu Family Foundation Scholarship Awardees . 15 Speaker Biographies . 17 Program Schedule . .25 Posters . 37 Abstracts . 53 Author Index . 130 PROGRAM and ABSTRACTS of the 32nd International Conference on Antiviral Research (ICAR) 2 ND DAILY International Conference SCHEDULE 32on Antiviral Research (ICAR) SUNDAY, MAY 12, 2019 › Women in Science Roundtable › Welcome and Keynote Lectures › Antonín Holý Memorial Award Lecture › Influenza Symposium › Opening Reception MONDAY, MAY 13, 2019 › Women in Science Award Lecture › Emerging Virus Symposium › Short Presentations 1 › Poster Session 1 › Retrovirus Symposium › ISAR Award of Excellence Presentation › PechaKucha Event with Introduction of First Time Attendees TUESDAY, MAY 14, 2019 › What’s New in Antiviral Research 1 › Short Presentations 2 & 3 › ISAR Award for Outstanding Contributions to the Society Presentation › Career Development Panel › William Prusoff Young Investigator Award Lecture › Medicinal Chemistry Symposium › Poster Session 2 › Networking Reception WEDNESDAY, MAY 15, 2019 › Gertrude Elion Memorial Award Lecture › What’s New in Antiviral Research 2 › Shotgun Oral -
To Ebola Reston
WHO/HSE/EPR/2009.2 WHO experts consultation on Ebola Reston pathogenicity in humans Geneva, Switzerland 1 April 2009 EPIDEMIC AND PANDEMIC ALERT AND RESPONSE WHO experts consultation on Ebola Reston pathogenicity in humans Geneva, Switzerland 1 April 2009 © World Health Organization 2009 All rights reserved. The designations employed and the presentation of the material in this publication do not imply the expression of any opinion whatsoever on the part of the World Health Organization concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. Dotted lines on maps represent approximate border lines for which there may not yet be full agreement. The mention of specific companies or of certain manufacturers’ products does not imply that they are endorsed or recommended by the World Health Organization in preference to others of a similar nature that are not mentioned. Errors and omissions excepted, the names of proprietary products are distin- guished by initial capital letters. All reasonable precautions have been taken by the World Health Organization to verify the information contained in this publication. However, the published material is being distributed without warranty of any kind, either express or implied. The responsibility for the interpretation and use of the material lies with the reader. In no event shall the World Health Organization be liable for damages arising from its use. This publication contains the collective views of an international group of experts and does not necessarily represent the decisions or the policies of the World Health Organization. -
How Severe and Prevalent Are Ebola and Marburg Viruses?
Nyakarahuka et al. BMC Infectious Diseases (2016) 16:708 DOI 10.1186/s12879-016-2045-6 RESEARCHARTICLE Open Access How severe and prevalent are Ebola and Marburg viruses? A systematic review and meta-analysis of the case fatality rates and seroprevalence Luke Nyakarahuka1,2,5* , Clovice Kankya2, Randi Krontveit3, Benjamin Mayer4, Frank N. Mwiine2, Julius Lutwama5 and Eystein Skjerve1 Abstract Background: Ebola and Marburg virus diseases are said to occur at a low prevalence, but are very severe diseases with high lethalities. The fatality rates reported in different outbreaks ranged from 24–100%. In addition, sero-surveys conducted have shown different seropositivity for both Ebola and Marburg viruses. We aimed to use a meta-analysis approach to estimate the case fatality and seroprevalence rates of these filoviruses, providing vital information for epidemic response and preparedness in countries affected by these diseases. Methods: Published literature was retrieved through a search of databases. Articles were included if they reported number of deaths, cases, and seropositivity. We further cross-referenced with ministries of health, WHO and CDC databases. The effect size was proportion represented by case fatality rate (CFR) and seroprevalence. Analysis was done using the metaprop command in STATA. Results: The weighted average CFR of Ebola virus disease was estimated to be 65.0% [95% CI (54.0–76.0%), I2 = 97.98%] whereas that of Marburg virus disease was 53.8% (26.5–80.0%, I2 = 88.6%). The overall seroprevalence of Ebola virus was 8.0% (5.0%–11.0%, I2 = 98.7%), whereas that for Marburg virus was 1.2% (0.5–2.0%, I2 = 94.8%). -
Ebola Virus Disease and Clinical Care Part I: History, Transmission, and Clinical Presentation
Ebola Virus Disease and Clinical Care Part I: History, Transmission, and Clinical Presentation This lecture is on Ebola virus disease (EVD) and clinical care. This is part one of a three-part lecture on this topic. Preparing Healthcare Workers to Work in Ebola Treatment Units (ETUs) in Africa This lecture will focus on EVD in the West African setting. Ebola Virus Disease and Clinical Care: The training and information you receive in this course will Part I: History, Transmission, and Clinical not cover the use of certain interventions such as intubation Presentation or dialysis which are not available in West African Ebola Treatment Units (ETUs). You will need supplemental training This presentation is current as of December 2014. This presentation contains materials from Centers for Disease Control and to care for patients appropriately in countries where advanced Prevention (CDC), Médecins Sans Frontières (MSF), and World Health Organization (WHO). care is available. U.S. Department of Health and Human Services U.S. Department of Health and Human Services Centers for Disease Control and Prevention Centers for Disease Control and Prevention version 12.03.2014 The learning objectives for this lecture are to: Learning Objectives ▶ Describe the routes of Ebola virus transmission Describe the routes of Ebola virus transmission Explain when and how patients are infectious ▶ Explain when and how patients are infectious Describe the clinical features of patients with Ebola ▶ Describe screening criteria for Ebola virus disease Describe the clinical features of patients with Ebola (EVD) used in West Africa Explain how to identify patients with suspected ▶ Describe screening criteria for EVD used in West Africa EVD who present to the ETU ▶ Explain how to identify patients with suspected EVD who present to the ETU This presentation contains materials from CDC, MSF, and WHO 2 A number of different viruses cause viral hemorrhagic fever. -
Development of an Antibody Cocktail for Treatment of Sudan Virus Infection
Development of an antibody cocktail for treatment of Sudan virus infection Andrew S. Herberta,b, Jeffery W. Froudea,1, Ramon A. Ortiza, Ana I. Kuehnea, Danielle E. Doroskya, Russell R. Bakkena, Samantha E. Zaka,b, Nicole M. Josleyna,b, Konstantin Musiychukc, R. Mark Jonesc, Brian Greenc, Stephen J. Streatfieldc, Anna Z. Wecd,2, Natasha Bohorovae, Ognian Bohorove, Do H. Kime, Michael H. Paulye, Jesus Velascoe, Kevin J. Whaleye, Spencer W. Stoniera,b,3, Zachary A. Bornholdte, Kartik Chandrand, Larry Zeitline, Darryl Sampeyf, Vidadi Yusibovc,4, and John M. Dyea,5 aVirology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702; bThe Geneva Foundation, Tacoma, WA 98402; cFraunhofer USA Center for Molecular Biotechnology, Newark, DE 19711; dDepartment of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461; eMapp Biopharmaceutical, Inc., San Diego, CA 92121; and fBioFactura, Inc., Frederick, MD 21701 Edited by Y. Kawaoka, University of Wisconsin–Madison/University of Tokyo, Madison, WI, and approved December 30, 2019 (received for review August 28, 2019) Antibody-based therapies are a promising treatment option for antibody-based immunotherapies. Subsequently, several indepen- managing ebolavirus infections. Several Ebola virus (EBOV)-specific dent groups reported the postexposure efficacy of monoclonal and, more recently, pan-ebolavirus antibody cocktails have been antibody (mAb)-based immunotherapies against EVD in ma- described. Here, we report the development and assessment of a caques when administered as mixtures of two or more mAbs (14–17). Sudan virus (SUDV)-specific antibody cocktail. We produced a panel These landmark studies demonstrated that combination mAb- of SUDV glycoprotein (GP)-specific human chimeric monoclonal based immunotherapies are a viable treatment option for EVD antibodies (mAbs) using both plant and mammalian expression and spurred therapeutic antibody development for filoviruses. -
Understanding Ebola
Understanding Ebola With the arrival of Ebola in the United States, it's very easy to develop fears that the outbreak that has occurred in Africa will suddenly take shape in your state and local community. It's important to remember that unless you come in direct contact with someone who is infected with the disease, you and your family will remain safe. State and government agencies have been making preparations to address isolated cases of infection and stop the spread of the disease as soon as it has been positively identified. Every day, the Centers of Disease Control and Prevention (CDC) is monitoring developments, testing for suspected cases and safeguarding our lives with updates on events and the distribution of educational resources. Learning more about Ebola and understanding how it's contracted and spread will help you put aside irrational concerns and control any fears you might have about Ebola severely impacting your life. Use the resources below to help keep yourself calm and focused during this unfortunate time. Ebola Hemorrhagic Fever Ebola hemorrhagic fever (Ebola HF) is one of numerous Viral Hemorrhagic Fevers. It is a severe, often fatal disease in humans and nonhuman primates (such as monkeys, gorillas, and chimpanzees). Ebola HF is caused by infection with a virus of the family Filoviridae, genus Ebolavirus. When infection occurs, symptoms usually begin abruptly. The first Ebolavirus species was discovered in 1976 in what is now the Democratic Republic of the Congo near the Ebola River. Since then, outbreaks have appeared sporadically. There are five identified subspecies of Ebolavirus. -
Taï Forest Virus Does Not Cause Lethal Disease in Ferrets
microorganisms Article Taï Forest Virus Does Not Cause Lethal Disease in Ferrets Zachary Schiffman 1,2,† , Feihu Yan 3,†, Shihua He 2, Kevin Tierney 2, Wenjun Zhu 2 , Karla Emeterio 1,2, Huajun Zhang 1, Logan Banadyga 2,* and Xiangguo Qiu 2 1 Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB R3E 0J9, Canada; [email protected] (Z.S.); [email protected] (K.E.); [email protected] (H.Z.) 2 Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada; [email protected] (S.H.); [email protected] (K.T.); [email protected] (W.Z.); [email protected] (X.Q.) 3 Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130122, China; [email protected] * Correspondence: [email protected] † These authors contributed equally. Abstract: Filoviruses are zoonotic, negative-sense RNA viruses, most of which are capable of causing severe disease in humans and nonhuman primates, often with high case fatality rates. Among these viruses, those belonging to the Ebolavirus genus—particularly Ebola virus, Sudan virus, and Bundibugyo virus—represent some of the most pathogenic to humans. Taï Forest virus (TAFV) is thought to be among the least pathogenic ebolaviruses; however, only a single non-fatal case has been documented in humans, in 1994. With the recent success of the ferret as a lethal model for a number of ebolaviruses, we set out to evaluate its suitability as a model for TAFV. Our results demonstrate that, unlike other ebolaviruses, TAFV infection in ferrets does not result in lethal disease. -
Ebolavirus an Animals
Ebolavirus and Animals Our knowledge to date and how animal health can support the public health emergency Ebolavirus and Animals – Our knowledge to date and how animal health can support the public health emergency • 29 May 2018 Speakers • Lee Myers • Cyprien Felix Biaou Manager a.I. Emergency Management Livestock Development Officer, FAO Centre for Animal Health (EMC-AH) sub-regional Office for Central Africa Coordinator of FAO’s Ebola Incident (FAO-SFC) Coordination Group (ICG) • Claudia Pittiglio • Sophie von Dobschuetz Ecologist Global Surveillance coordinator (EPT-2) FAO Global Early Warning System (FAO- Emergency Prevention system for GLEWS) and Emerging Pandemic Threats transboundary Animal Diseases Programme Phase 2 (EPT-2) (EMPRES-AH) Ebolavirus and Animals – Our knowledge to date and how animal health can support the public health emergency • 29 May 2018 Webinar Content – Ebola and Animals • Presentation of FAO’s activities – Global level (Lee MYERS) – Regional and country levels (Cyprien BIAOU) • Susceptibility of animal species – Wildlife (Claudia PITTIGLIO) – Domestic (Sophie VON DOBSCHUETZ) • Availability of diagnostic tests for animal samples (Sophie VON DOBSCHUETZ) • What AH agencies and partners are doing (Sophie VON DOBSCHUETZ) Ebolavirus and Animals – Our knowledge to date and how animal health can support the public health emergency • 29 May 2018 3 Context of this webinar: <Phylogeny of the family Filoviridae ZEBOV Current outbreak in the Democratic Republic of the Congo (DRC) © Basicmedical Key Ebolavirus and Animals – Our knowledge to date and how animal health can support the public health emergency • 29 May 2018 FAO Ebola Incident Coordination Group (ICG) • In the spirit of One Health, FAO is supporting the efforts of the public health sector in response to the EVD outbreak in the Democratic Republic of the Congo. -
Principais Métodos De Diagnóstico E Tratamento Da Doença Causada Pelo Vírus Ebola
Revista Ciencia & Inovação - FAM - V.5, N.1 - JUN - 2020 PRINCIPAIS MÉTODOS DE DIAGNÓSTICO E TRATAMENTO DA DOENÇA CAUSADA PELO VÍRUS EBOLA Jaqueline Pereira Fernandes Patricia Ucelli Simioni Graduada de Biomedicina, Faculdade de Americana (FAM) Bióloga, mestre e doutora em Imunologia, [email protected] Faculdade de Americana, [email protected] Monna Abdel Latif Elaine Cristina Berro Graduada de Biomedicina, Faculdade de Americana (FAM) Engenheira Ambiental, especialista em Microbiologia, [email protected] Faculdade de americana (FAM), [email protected] Leila Ugrinovich Bióloga, mestre e doutora em Microbiologia, Faculdade de Americana, [email protected] RESUMO ABSTRACT Ebola é uma doença grave causada pelo Ebola vírus, Ebola is a serious disease caused by the Ebola virus da família Filoviridae, que apresenta altos índices of the Filoviridae family has high mortality rates. Bat, de mortalidade. Animais como morcego, chimpanzé chimpanzee and monkey may be hosts of the virus. e macaco podem ser hospedeiros do vírus. O The diagnosis is only made by specialized laboratories, diagnóstico adequado é feito apenas em laboratórios based on the isolation of viral RNA. However, especializados, a partir do isolamento do RNA viral. current diagnostic methodologies include real-time Entretanto, metodologias atuais de diagnóstico incluem polymerase chain reaction (PRC) and assessment reação de polimerase em cadeia (PRC) em tempo real of immunoglobulins (Ig) M and G Currently available e avaliação de imunoglobulinas (Ig) M e G As terapias therapies are palliative. New therapies are already disponíveis atualmente são paliativas. Novas terapias já being tested in humans and promise advances in the estão em fase de testes em seres humanos e prometem treatment of the disease, although their safety and avanços no tratamento da doença, embora ainda não effectiveness have not yet been proven.