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Issue Full File ISSN 1308-5301 Print ISSN 1308-8084 Online Biological Diversity and Conservation CİLT / VOLUME 5 SAYI / NUMBER 1 NİSAN / APRIL 2012 Biyolojik Çeşitlilik ve Koruma Üzerine Yayın Yapan Hakemli Uluslararası Bir Dergidir An International Journal is About Biological Diversity and Conservation With Refree BioDiCon Biyolojik Çeşitlilik ve Koruma Biological Diversity and Conservation Biyolojik Çeşitlilik ve Koruma Üzerine Yayın Yapan Hakemli Uluslararası Bir Dergidir An International Journal is About Biological Diversity and Conservation With Refree Cilt / Volume 5, Sayı / Number 1, Nisan/April 2012 Editör / Editor-in-Chief: Ersin YÜCEL ISSN 1308-5301 Print ISSN 1308-8084 Online Açıklama “Biological Diversity and Conservation”, biyolojik çeşitlilik, koruma, biyoteknoloji, çevre düzenleme, tehlike altındaki türler, tehlike altındaki habitatlar, sistematik, vejetasyon, ekoloji, biyocoğrafya, genetik, bitkiler, hayvanlar ve mikroorganizmalar arasındaki ilişkileri konu alan orijinal makaleleri yayınlar. Tanımlayıcı yada deneysel ve sonuçları net olarak belirlenmiş deneysel çalışmalar kabul edilir. Makale yazım dili Türkçe veya İngilizce’dir. Yayınlanmak üzere gönderilen yazı orijinal, daha önce hiçbir yerde yayınlanmamış olmalı veya işlem görüyor olmamalıdır. Yayınlanma yeri Türkiye’dir. Bu dergi yılda üç sayı yayınlanır. Description “Biological Diversity and Conservation” publishes original articles on biological diversity, conservation, biotechnology, environmental management, threatened of species, threatened of habitats, systematics, vegetation science, the ecology, biogeography, genetics and interactions among plants and animals or microorganisms. Descriptive or experimental studies presenting clear research questions are accepted. The submitted paper must be original and unpublished or under consideration for publication. Manuscripts in English or in Turkish languages are welcome. Printed in Turkey. This journal is published triannullay. Dergiyi tarayan veri tabanları / Abstracted-Indexed in DOAJ-Directory of Open Access Journals; Bibliotheken; Buscador de Archives; Dayang Journal System; EBSCO Publishings databases; Google Scholar; HealthHaven; HKU Scholars Hub.; ICAAP-database; Index Copernicus; Journal Directory, News-of-Science; OhioLINK Databases-OPC4-Online-Katalog der Bibliothek der Fachhochschule Anhalt; Online-Katalog der UB Clausthal; Paper Search Engine; ProQest-Central To Rechearch Araund The World; Thomson Reuters; Ulakbim; ULRICH’S-The Global Source for Periodicals Kütüphaneler / Libraries EZB Electronic Journals Library; Feng Chia University Library; GAZİ Gazi University Library; HEC-National Digital Library; Kaohsinug Medical University Library; KYBELE Anadolu University Library; Libros PDF; National Cheng Kung University Library; National ILAN University Library; Shih Hsin University Library; Smithsonian Institution Libraries; The Ohio Library and Information NetWork; Vaughan Memorial Library. Index Copernicus International, IC Value = 9.00 (2010) Makale yazım kuralları ve dergi ile ilgili diğer ayrıntılar için ” http:// www.biodicon.com“ adresini ziyaret ediniz / Please visit ” http:// www.biodicon.com“ for instructions about articles and all of the details about journal Yazışma Adresi / Correspondance Adres; Prof. Ersin YÜCEL, P.K. 86, PTT Merkez, 26010 Eskişehir / Turkey E-posta : [email protected] ; http://www.biodicon.com Biyolojik Çeşitlilik ve Koruma/Biological Diversity and Conservation ISSN 1308-5301 Print; ISSN 1308-8084 Online © Biyolojik Çeşitlilik ve Koruma 2008 / Biological Diversity and Conservation 2008 Sahibi / Publisher : Ersin YÜCEL Bu dergi yılda üç sayı yayınlanır. Yayınlanma yeri Eskişehir/Türkiye’dir. / This journal is published three numbers in a year. Printed in Eskişehir/Turkey. Dergide yayınlanan makalelerin her türlü sorumluluğu yazarlarına aittir / All sorts of responsibilities of the articles published in this journal are belonging to the authors Editör / Editor-In-Chief : Prof.Dr. Ersin YÜCEL Yayın Kurulu / Editorial Board İbrahim Türkekul, Tokat (Turkey) Amed A. Azmani, Tangier (Morocco) İlkin Yücel Şengün, İzmir (Turkey) Ahmet Aksoy, Kayseri (Turkey) İskender Gülle, Burdur (Turkey) Ali Dönmez, Ankara (Turkey) İsmet Uysal, Çanakkale (Turkey) Atilla Ocak, Eskişehir (Turkey) İsmühan Potoğlu, Eskişehir (Turkey) Cemil Ata, İstanbul (Turkey) Latif Kurt, Ankara (Turkey) Cheruth Abdul Jaleel, Al-Ain (United Arab Emirates) Marjan Komnenov, Skopje (Republic of Macedonia) Hakan Ulukan, Ankara (Turkey) Mirza Barjees Baig, Riyadh (Kingdom of Saudi Arabia) Hayri Duman, Ankara (Turkey) Mohammed Merzouki, Fez (Morocco) Ignacy Kitowski, Lublin (Poland) Muhammad Iqbal, Nowshera, NWFP (Pakistan) Iqrar Ahmad Khan, Faisalabad (Pakistan) Muhammad Sarwar, Tando Jam (Pakistan) Ian C. Hedge, Edinburgh, (Scotland, UK) Muhammad Yasin Ashraf, Faisalabad (Pakistan) Lyutsiya Aubakirova, Astana (Kazakhstan) Muhammet Örem, Zonguldak (Turkey) Kani Işık, Antalya (Turkey) Murad Aydın Şanda, Konya (Turkey) Masoud Hedayatifard, Ghaemshahr (Iran) Musa Doğan, Ankara (Turkey) M.N.V. Prasad, Hyderabad (India) Mustafa Işıloğlu, Muğla (Turkey) Mecit Vural, Ankara, (Turkey) Mustafa Kargıoğlu, Afyon (Turkey) Mirza Barjees Baig, Riyadh (Kingdom of Saudi Arabia) Mustafa Küçüködük, Konya (Turkey) Metin Sarıbaş, Bartın (Turkey) Mustafa Yamaç, Eskişehir (Turkey) Muhammad Ashraf, Faisalabad (Pakistan) Müge Seyitoğlu, İstanbul (Turkey) Münir Öztürk, İzmir (Turkey) Naime Arslan, Eskişehir (Turkey) Özcan Seçmen, İzmir (Turkey) Nedim Değirmenci, Eskişehir (Turkey) Rıdvan Tuncel, Eskişehir (Turkey) Nuri Öner, Çankırı (Turkey) Yunus Doğan, İzmir (Turkey) Nülifer Erkasap, Eskişehir ------------------------------------------------------------- Öner Demirel, Trabzon (Turkey) Hakemler / Reviewers Perihan Güler, Kırıkkale (Turkey) Abdullah Kaya, Karaman (Turkey) Saleem Ahmad, Islamabad (Pakistan) Ahmet Emre Yaprak, Ankara, (Turkey) Seher Karaman Erkul, Aksaray (Turkey) Amed Aarab Azmani, Tangier (Morocco) Selima Khatun, West Bengal (India) Amed Aarab Azmani, Tangier (Morocco) Serdar Aslan, Düzce (Turkey) Aykut Güvensen, İzmir (Turkey) Sezgin Ayan, Kastamonu (Turkey) Cahit Doğan, Ankara (Turkey) Sezgin Çelik, Kırıkkale (Turkey) Dalila Bousta, Taounate (Morocco) Shyam Singh Yadav, Lae (Papua New Guinea) Dursun Yağız, Konya (Turkey) Snejana Doncheva, Sofia (Bulgaria ) Elif Yamaç, Eskişehir (Turkey) Sumaira Sahren, Islamabad (Pakistan) Emel Sözen, Eskişehir (Turkey) Sunil Kumar Khan, Magra,Hooghly (India) Ergin Hamzaoğlu, Yozgat (Turkey) Süleyman Başlar, İzmir (Turkey) Faik Ahmet Karavelioğlu, Ankara (Turkey) Şinasi Yıldırımlı, Ankara (Turkey) Fatih Demirci, Eskişehir (Turkey) Tamer Keçeli, Çankırı (Turkey) Fatma Güneş Koçyiğit, Kars (Turkey) Tarık Danışman, Kırıkkale (Turkey) Fazle Subhan, Peshawar-Tarnab (Pakistan) Tuğba Bayrak Özbucak, Ordu (Turkey) Gıyasettin Kaşık, Konya(Turkey) Turan Özdemir, Trabzon (Turkey) Gönül Kaynak, Bursa (Turkey) Tülay Ezer, Niğde (Turkey) Gülendam Tümen, Bursa (Turkey) Uğur Çakılcıoğlu, Elazığ (Turkey) Güray Uyar, Zonguldak, (Turkey) Ünal Özelmas, Eskişehir (Turkey) Haider Abbas, Karachi (Pakistan) Yavuz Bağcı, Konya (Turkey) Halil Solak, Ula/ Muğla (Turkey) Yeşim Kara, Denizli (Turkey) Hasan Özçelik, Isparta (Turkey) Yusuf Menemen, Kırıkkale (Turkey) Hatice Kiremit Özenoğlu, Aydın (Turkey) Zafer Cemal Özkan, Trabzon (Turkey) Hulusi Malyer, Bursa (Turkey) Zeki Aytaç, Ankara (Turkey) Hüseyin Dural, Konya (Turkey) ----------------------------------------------------------------------------------------------------------------------------------------------- Editörlük Ofisi/Editorial Office; Alper YARGIÇ, Hilal ERSOY, Mine YÜCEL Kapak Düzeni/ Cover Layout; Mine YÜCEL Kapak Resmi/ Cover Image: . Ersin YÜCEL, Dilge YÜCEL Dizgi/Compositor; Mine YÜCEL Baskı/Printing Press; Haser Dijital, Eskişehir/Turkey Veb Danışma/Web Consultant ; Alper YARGIÇ www.biodicon.com Biological Diversity and Conservation ISSN 1308-8084 Online; ISSN 1308-5301 Print 5/1 (2012) 1-4 Research article/Araştırma makalesi Time dependent cytotoxic role of Homalothecium sericeum extracts on glioma Pinar OZTOPCU-VATAN *1, Selda KABADERE2, Ruhi UYAR2, Filiz SAVAROGLU1, Gökhan KUS3 1 Eskisehir Osmangazi University, 1Faculty of Arts and Sciences, Department of Biology, Eskisehir, Turkey. 2 Eskisehir Osmangazi University, Faculty of Medicine, Department of Physiology, Eskisehir, Turkey. 3 Eskisehir Osmangazi University, Graduate School of Medical Science, Eskisehir, Turkey. Abstract Bryophytes have been used as medicinal plants for more than 400 years in China, Europe and North America. There is also evidence confirming the antibiotic and anticancer activity of Bryophytes against, prokaryotes, fungi and different cancer cells. The purpose of the current study was to investigate cytotoxic property of Homalothecium sericeum (hedw.) schimp., which is a bryophyte, extracts on rat glioma (C6) cells for 48 hrs, in vitro. We first collected two different (acetone and A) extracts from H. sericeum by two different extraction processes. C6 cells were seeded in 96 well plates (2x104 cells/well) and incubated for 24 hrs. Following this incubation period, the medium was replaced with only medium (control) or medium with extracts at concentrations of 0.17, 1.7, 17, 85 or 170 µg/mL for 48 hrs. Cytotoxicity was determined by using 3-(4,5-dimethylthiazol-2yl)- 2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Acetone extract of H. sericeum at 0.17, 1.7 and 17 µg/mL concentrations did not change the survival rate of C6, but 85 and 170 µg/mL inhibited about 16 %
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