Illuminating the Black Box of B12 Biosynthesis Harry A
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Enzymatic Encoding Methods for Efficient Synthesis Of
(19) TZZ__T (11) EP 1 957 644 B1 (12) EUROPEAN PATENT SPECIFICATION (45) Date of publication and mention (51) Int Cl.: of the grant of the patent: C12N 15/10 (2006.01) C12Q 1/68 (2006.01) 01.12.2010 Bulletin 2010/48 C40B 40/06 (2006.01) C40B 50/06 (2006.01) (21) Application number: 06818144.5 (86) International application number: PCT/DK2006/000685 (22) Date of filing: 01.12.2006 (87) International publication number: WO 2007/062664 (07.06.2007 Gazette 2007/23) (54) ENZYMATIC ENCODING METHODS FOR EFFICIENT SYNTHESIS OF LARGE LIBRARIES ENZYMVERMITTELNDE KODIERUNGSMETHODEN FÜR EINE EFFIZIENTE SYNTHESE VON GROSSEN BIBLIOTHEKEN PROCEDES DE CODAGE ENZYMATIQUE DESTINES A LA SYNTHESE EFFICACE DE BIBLIOTHEQUES IMPORTANTES (84) Designated Contracting States: • GOLDBECH, Anne AT BE BG CH CY CZ DE DK EE ES FI FR GB GR DK-2200 Copenhagen N (DK) HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI • DE LEON, Daen SK TR DK-2300 Copenhagen S (DK) Designated Extension States: • KALDOR, Ditte Kievsmose AL BA HR MK RS DK-2880 Bagsvaerd (DK) • SLØK, Frank Abilgaard (30) Priority: 01.12.2005 DK 200501704 DK-3450 Allerød (DK) 02.12.2005 US 741490 P • HUSEMOEN, Birgitte Nystrup DK-2500 Valby (DK) (43) Date of publication of application: • DOLBERG, Johannes 20.08.2008 Bulletin 2008/34 DK-1674 Copenhagen V (DK) • JENSEN, Kim Birkebæk (73) Proprietor: Nuevolution A/S DK-2610 Rødovre (DK) 2100 Copenhagen 0 (DK) • PETERSEN, Lene DK-2100 Copenhagen Ø (DK) (72) Inventors: • NØRREGAARD-MADSEN, Mads • FRANCH, Thomas DK-3460 Birkerød (DK) DK-3070 Snekkersten (DK) • GODSKESEN, -
An Efficient Synthesis of Porphyrins with Different Meso Substituents That Avoids Scrambling in Aqueous Media
An Efficient Synthesis of Porphyrins with Different meso Substituents that Avoids Scrambling in Aqueous Media Agnieszka Nowak-Krol, Rémi Plamont, Gabriel Canard, J.A. Edzang, Daniel T. Gryko, Teodor Silviu Balaban To cite this version: Agnieszka Nowak-Krol, Rémi Plamont, Gabriel Canard, J.A. Edzang, Daniel T. Gryko, et al.. An Efficient Synthesis of Porphyrins with Different meso Substituents that Avoids Scrambling inAque- ous Media. Chemistry - A European Journal, Wiley-VCH Verlag, 2015, 21 (4), pp.1488-1498. 10.1002/chem.201403677. hal-01130057 HAL Id: hal-01130057 https://hal.archives-ouvertes.fr/hal-01130057 Submitted on 7 Feb 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. An Efficient Synthesis of Porphyrins with Different Meso Substituents that Avoids Scrambling in Aqueous Media Agnieszka Nowak-Król,a,† Rémi Plamont,b,† Gabriel Canard,b,c Judicaelle Andeme Edzang,b,c Daniel T. Grykoa,* and Teodor Silviu Balabanb,* To the memory of Alan Roy Katritzky, magister of heterocyclic chemistry [a] Institute of Organic Chemistry of the Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland E-mail: [email protected] [b] Aix Marseille Université, Centrale Marseille, CNRS, Institut des Sciences Moléculaires de Marseille (iSm2), UMR 7313, Chirosciences, Avenue Escadrille Normandie Niemen, St. -
Magnesium-Protoporphyrin Chelatase of Rhodobacter
Proc. Natl. Acad. Sci. USA Vol. 92, pp. 1941-1944, March 1995 Biochemistry Magnesium-protoporphyrin chelatase of Rhodobacter sphaeroides: Reconstitution of activity by combining the products of the bchH, -I, and -D genes expressed in Escherichia coli (protoporphyrin IX/tetrapyrrole/chlorophyll/bacteriochlorophyll/photosynthesis) LUCIEN C. D. GIBSON*, ROBERT D. WILLOWSt, C. GAMINI KANNANGARAt, DITER VON WETTSTEINt, AND C. NEIL HUNTER* *Krebs Institute for Biomolecular Research and Robert Hill Institute for Photosynthesis, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, United Kingdom; and tCarlsberg Laboratory, Department of Physiology, Gamle Carlsberg Vej 10, DK-2500 Copenhagen Valby, Denmark Contributed by Diter von Wettstein, November 14, 1994 ABSTRACT Magnesium-protoporphyrin chelatase lies at Escherichia coli and demonstrate that the extracts of the E. coli the branch point of the heme and (bacterio)chlorophyll bio- transformants can convert Mg-protoporphyrin IX to Mg- synthetic pathways. In this work, the photosynthetic bacte- protoporphyrin monomethyl ester (20, 21). Apart from posi- rium Rhodobacter sphaeroides has been used as a model system tively identifying bchM as the gene encoding the Mg- for the study of this reaction. The bchH and the bchI and -D protoporphyrin methyltransferase, this work opens up the genes from R. sphaeroides were expressed in Escherichia coli. possibility of extending this approach to other parts of the When cell-free extracts from strains expressing BchH, BchI, pathway. In this paper, we report the expression of the genes and BchD were combined, the mixture was able to catalyze the bchH, -I, and -D from R. sphaeroides in E. coli: extracts from insertion of Mg into protoporphyrin IX in an ATP-dependent these transformants, when combined in vitro, are highly active manner. -
AOP 131: Aryl Hydrocarbon Receptor Activation Leading to Uroporphyria
Organisation for Economic Co-operation and Development DOCUMENT CODE For Official Use English - Or. English 1 January 1990 AOP 131: Aryl hydrocarbon receptor activation leading to uroporphyria Short Title: AHR activation-uroporphyria This document was approved by the Extended Advisory Group on Molecular Screening and Toxicogenomics in June 2018. The Working Group of the National Coordinators of the Test Guidelines Programme and the Working Party on Hazard Assessment are invited to review and endorse the AOP by 29 March 2019. Magdalini Sachana, Administrator, Hazard Assessment, [email protected], +(33- 1) 85 55 64 23 Nathalie Delrue, Administrator, Test Guidelines, [email protected], +(33-1) 45 24 98 44 This document, as well as any data and map included herein, are without prejudice to the status of or sovereignty over any territory, to the delimitation of international frontiers and boundaries and to the name of any territory, city or area. 2 │ Foreword This Adverse Outcome Pathway (AOP) on Aryl hydrocarbon receptor activation leading to uroporphyria, has been developed under the auspices of the OECD AOP Development Programme, overseen by the Extended Advisory Group on Molecular Screening and Toxicogenomics (EAGMST), which is an advisory group under the Working Group of the National Coordinators for the Test Guidelines Programme (WNT). The AOP has been reviewed internally by the EAGMST, externally by experts nominated by the WNT, and has been endorsed by the WNT and the Working Party on Hazard Assessment (WPHA) in xxxxx. Through endorsement of this AOP, the WNT and the WPHA express confidence in the scientific review process that the AOP has undergone and accept the recommendation of the EAGMST that the AOP be disseminated publicly. -
On Tuning the Fluorescence Emission of Porphyrin Free Bases Bonded to the Pore Walls of Organo-Modified Silica
Molecules 2014, 19, 2261-2285; doi:10.3390/molecules19022261 OPEN ACCESS molecules ISSN 1420-3049 www.mdpi.com/journal/molecules Article On Tuning the Fluorescence Emission of Porphyrin Free Bases Bonded to the Pore Walls of Organo-Modified Silica Rosa I. Y. Quiroz-Segoviano 1, Iris N. Serratos 1, Fernando Rojas-González 1, Salvador R. Tello-Solís 1, Rebeca Sosa-Fonseca 2, Obdulia Medina-Juárez 1, Carmina Menchaca-Campos 3 and Miguel A. García-Sánchez 1,* 1 Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, Vicentina, D. F. 09340, Mexico 2 Departamento de Física, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, Vicentina, D. F. 09340, Mexico 3 Centro de Investigación en Ingeniería y Ciencias Aplicadas, UAEM, Av. Universidad 1001, Col. Chamilpa, C.P. 62209, Cuernavaca Mor., Mexico * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +52-55-5804-4677; Fax: +52-55-5804-4666. Received: 24 December 2013; in revised form: 29 January 2014 / Accepted: 7 February 2014 / Published: 21 February 2014 Abstract: A sol-gel methodology has been duly developed in order to perform a controlled covalent coupling of tetrapyrrole macrocycles (e.g., porphyrins, phthalocyanines, naphthalocyanines, chlorophyll, etc.) to the pores of metal oxide networks. The resulting absorption and emission spectra intensities in the UV-VIS-NIR range have been found to depend on the polarity existing inside the pores of the network; in turn, this polarization can be tuned through the attachment of organic substituents to the tetrapyrrrole macrocycles before bonding them to the pore network. -
Characterisation, Classification and Conformational Variability Of
Characterisation, Classification and Conformational Variability of Organic Enzyme Cofactors Julia D. Fischer European Bioinformatics Institute Clare Hall College University of Cambridge A thesis submitted for the degree of Doctor of Philosophy 11 April 2011 This dissertation is the result of my own work and includes nothing which is the outcome of work done in collaboration except where specifically indicated in the text. This dissertation does not exceed the word limit of 60,000 words. Acknowledgements I would like to thank all the members of the Thornton research group for their constant interest in my work, their continuous willingness to answer my academic questions, and for their company during my time at the EBI. This includes Saumya Kumar, Sergio Martinez Cuesta, Matthias Ziehm, Dr. Daniela Wieser, Dr. Xun Li, Dr. Irene Pa- patheodorou, Dr. Pedro Ballester, Dr. Abdullah Kahraman, Dr. Rafael Najmanovich, Dr. Tjaart de Beer, Dr. Syed Asad Rahman, Dr. Nicholas Furnham, Dr. Roman Laskowski and Dr. Gemma Holli- day. Special thanks to Asad for allowing me to use early development versions of his SMSD software and for help and advice with the KEGG API installation, to Roman for knowing where to find all kinds of data, to Dani for help with R scripts, to Nick for letting me use his E.C. tree program, to Tjaart for python advice and especially to Gemma for her constant advice and feedback on my work in all aspects, in particular the chemistry side. Most importantly, I would like to thank Prof. Janet Thornton for giving me the chance to work on this project, for all the time she spent in meetings with me and reading my work, for sharing her seemingly limitless knowledge and enthusiasm about the fascinating world of enzymes, and for being such an experienced and motivational advisor. -
16Th March 2020 Blood Revised
Blood is the fluid circulating in a closed system of blood vessels and the chambers of the heart It is the medium which transports substances from one part of the body to the other Blood is composed of Plasma Platelets Cells WBCs RBCs (Erythrocytes) Hemoglobin (Hb) is red , oxygen carrying pigment present exclusively in erythrocytes HEMOGLOBIN A conjugated protein containing Globin Protein part ( 4 polypeptide chains- ) 96% of the total Hb mass Varies from species to species( species specificity) Heme Non protein (prosthetic group) Red colour Iron containing tetrapyrrole porphyrin derivative 4% of the total Hb mass Reversibly binds Oxygen Structure of Heme An Iron –porphyrin (Protoporphrin IX) compound with tetrapyrrole structure Protoporphyrin IX consists of 4 pyrrole rings combined through — CH= bridges (methyne bridges) The methyne bridges are referred as α,β,γ, and δ. The 2 Hydrogen atoms in the –NH groups pyrrole rings (II & IV) are replaced by Ferrous( Fe++) . The four pyrrole rings present in the porphyrin molecule are designated as I,II,III & IV . Each of these four rings has 2 groups attached to them M = Methyl –CH3 V = Vinyl – CH=CH2 P = Propionyl - CH2 - CH2 - COOH . The Fe++ can form 2 additional bonds .One of these position is linked internally (5th linkage ) to nitrogen of imidazole ring of Histidine of the Globin polypeptide chains . Other position is available to bind Oxygen Heme is the most prevalent metalloporphyrin in humans Common prosthetic group in Hemoglobin — Transport of O2 in blood Myoglobin — Storage of O2 in muscles Cytochromes — Part of electron transport chain Catalase — Degradation of H2O2 Tryptophan pyrolase — Oxidation of Tryptophan Cytochrome P450 — Hydroxylation of Xenobiotics HEME SYNTHESIS Major sites Liver Erythrocyte producing cells of bone marrow Rate of heme synthesis in liver is highly variable & depends upon size of heme pool while it is relatively constant in in bone marrow is relatively constant Mature RBC lack mitochondria and are unable to synthesize heme. -
Preparation of Tetrapyrrole-Amino Acid Covalent Complexes
I'lunt I'ht.siol.Ritx ltt'nt. 1996. -14 (3). 393-39lt Preparation of tetrapyrrole-amino acid covalent complexes Leszek Fiedorl'2*, Varda Rosenbach-Belkinl, Maruthi Sail and Avigdor Scherzl I BiochernistryDepartment. The Weizn-rannInstitute of Science.76100 Rehovot.Israel. I Prcscntaddress: Institute of Molecular BiologSr.Ja-ciellonian University. Al. Mickiewicza 3. 3 l- 120 Cracow. Poland. ':'Author to whom correspondenceshould bc addrcsscd(fax +48-12-336907:E-mail fiedor@)mol.uj.edu.pl) Abstract The presentedsynthetic approach towards chcn'rical modifications of chlorophylls(Chls) provides a perspectivcto construct model systems. where tetrapyrrole-aminoacid and tetrapyrrole-peptideinteractions coulcl be studied in covalent rnodel compor,rncls. The approach relies on thc lact that in Chls the | 7r propionic rcid sidc chain docs not participatc in the tetrapl'rroleii--electron system. It makes use of a plant enzvmechlolophyllase (EC 3.1.1.1,+).which lrr lilo and in yitrc catalysesreactions at this sidc function. The transesterilicationand hyclrolysisenzymatic rerctions are useful on a preparativescale. ln the transesterificationreaction. a desiredamino acid rcsiduc posscssirrgprimary hydloxyl group can be directly attachedto the propiorric acid side chain o1' Chl. This mcthod allows to replace the phytyl moiety in Chls n'ith seline. The r:rtherreaction. enzyrratic hydrolysis of Chls, yields chlorophyllides and opens a convenientroutc fbr furthcr rnodifications.If sufliciently mild synthetic mcthodsarc uscd. such as catalysisw,ith ,l-dimethyl arnino pyridine or activationwith N-hvdroxvsuccinimide.an arrino acid or peptide residuecan be covalentlybound to chlorophyllides' carboxylic group. lear,'ingthe essentialclectlonic structure of Chl intact. The activation w'ith N-hydroxvsuccininridcallows fbr the coupling cvrn in aqueous rncdia. -
Continuous Chlorophyll Degradation Accompanied by Chlorophyllide and Phytol Reutilization for Chlorophyll Synthesis in Synechocystis Sp
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Biochimica et Biophysica Acta 1767 (2007) 920–929 www.elsevier.com/locate/bbabio Continuous chlorophyll degradation accompanied by chlorophyllide and phytol reutilization for chlorophyll synthesis in Synechocystis sp. PCC 6803 ⁎ Dmitrii Vavilin, Wim Vermaas School of Life Sciences and Center for the Study of Early Events in Photosynthesis, Arizona State University, Box 874501, Tempe, AZ 85287, USA Received 3 January 2007; received in revised form 23 March 2007; accepted 27 March 2007 Available online 3 April 2007 Abstract Chlorophyll synthesis and degradation were analyzed in the cyanobacterium Synechocystis sp. PCC 6803 by incubating cells in the presence of 13C-labeled glucose or 15N-containing salts. Upon mass spectral analysis of chlorophyll isolated from cells grown in the presence of 13C-glucose for different time periods, four chlorophyll pools were detected that differed markedly in the amount of 13C incorporated into the porphyrin (Por) and phytol (Phy) moieties of the molecule. These four pools represent (i) unlabeled chlorophyll (12Por12Phy), (ii) 13C-labeled chlorophyll (13Por13Phy), and (iii, iv) chlorophyll, in which either the porphyrin or the phytol moiety was 13C-labeled, whereas the other constituent of the molecule remained unlabeled (13Por12Phy and 12Por13Phy). The kinetics of 12Por12Phy disappearance, presumably due to chlorophyll de- esterification, and of 13Por12Phy, 12Por13Phy, and 13Por13Phy accumulation due to chlorophyll synthesis provided evidence for continuous chlorophyll turnover in Synechocystis cells. The loss of 12Por12Phy was three-fold faster in a photosystem I-less strain than in a photosystem II- less strain and was accelerated in wild-type cells upon exposure to strong light. -
Bilirubin Suppresses Th17 Immunity in Colitis by Upregulating CD39
Bilirubin suppresses Th17 immunity in colitis by upregulating CD39 Maria Serena Longhi, … , Francisco J. Quintana, Simon C. Robson JCI Insight. 2017;2(9):e92791. https://doi.org/10.1172/jci.insight.92791. Research Article Gastroenterology Immunology Unconjugated bilirubin (UCB), a product of heme oxidation, has known immunosuppressant properties but the molecular mechanisms, other than antioxidant effects, remain largely unexplored. We note that UCB modulates T helper type 17 (Th17) immune responses, in a manner dependent upon heightened expression of CD39 ectonucleotidase. UCB has protective effects in experimental colitis, where it enhances recovery after injury and preferentially boosts IL-10 production by colonic intraepithelial CD4+ cells. In vitro, UCB confers immunoregulatory properties on human control Th17 cells, as reflected by increased levels of FOXP3 and CD39 with heightened cellular suppressor ability. Upregulation of CD39 by Th17 cells is dependent upon ligation of the aryl hydrocarbon receptor (AHR) by UCB. Genetic deletion of CD39, as in Entpd1–/– mice, or dysfunction of AHR, as inA hrd mice, abrogates these UCB salutary effects in experimental colitis. However, in inflammatory bowel disease (IBD) samples, UCB fails to confer substantive immunosuppressive properties upon Th17 cells, because of decreased AHR levels under the conditions tested in vitro. Immunosuppressive effects of UCB are mediated by AHR resulting in CD39 upregulation by Th17. Boosting downstream effects of AHR via UCB or enhancing CD39-mediated ectoenzymatic activity might provide therapeutic options to address development of Th17 dysfunction in IBD. Find the latest version: https://jci.me/92791/pdf RESEARCH ARTICLE Bilirubin suppresses Th17 immunity in colitis by upregulating CD39 Maria Serena Longhi,1 Marta Vuerich,1 Alireza Kalbasi,1 Jessica E. -
Functional Group Manipulation Usin Organoselenium Reagents
22 Reich Accounts of Chemical Research (25), bound covalently or by physical forces to the possibilities. One is that cosynthetase alters the con- enzyme system, is then converted into uro’gen-I11 (1) formation of the deaminase-bilane complex to direct by an intramolecular rearrangement which directly cyclization of 25 at C-16. There are indication^",^^ that affects only ring D and the two carbons which become deaminase associates with cosynthetase, and it has been C-15 and C-20. The nature of the intermediate between ~uggested”~,~~that cosynthetase acts as a “specifier the bilane and uro’gen-111 remains to be established, protein” in the way lactoalbumiri works during the and this leads to the concluding section. biosynthesis of lactose. The other possibility is that the Prospect. In the presence of deaminase alone, and bilane (25) is the product from deaminase but is then also nonenzymically, the cyclization of bilane (25) occurs the substrate for cosynthetase which brings about ring at C-19 to produce 26, leading to uro’gen-I (11). We closure with rearrangement to produce uro’gen-I11 suggest that in the presence of cosynthetase cyclization specifically. occurs at C-16 rather than at (2-19 (Scheme XIII). The Work is in hand on these aspects and on the problem postulated attack at C-16 would produce the spiro of the structure of the intermediate5’ between the bilane intermediate 27; the labeling arising from 25b and 25c and uro‘gen-111. It will be good to have the answers to is shown. Fragmentation and cyclization again as il- the few remaining questions. -
Vitamin B, and B,-Proteins Edited by Bernhard Krautler, Duilio Arigoni and Bernard T
Vitamin B, and B,-Proteins Edited by Bernhard Krautler, Duilio Arigoni and Bernard T. Golding Lectures presented at the 4th European Symposium on Vitamin B,, and B,,-Proteins @ W I LEY-VCH Weinheim - Chichester - New York - Toronto. Brisbane - Singapore This Page Intentionally Left Blank Vitamin B, and BIZ-Proteins Edited by B. Krautler, D. Arigoni and B.T. Golding 633 WILEY-VCH This Page Intentionally Left Blank Vitamin B, and B,-Proteins Edited by Bernhard Krautler, Duilio Arigoni and Bernard T. Golding Lectures presented at the 4th European Symposium on Vitamin B,, and B,,-Proteins @ W I LEY-VCH Weinheim - Chichester - New York - Toronto. Brisbane - Singapore Prof. Dr. B. Krautler Prof. Dr. D. Arigoni Prof. Dr. B.T. Golding Leopold-Franzens-Universitat ETH-Zurich Department of Chemistry Innsbruck Laboratoriuin fur University of Newcastle Institut fur Organische Chemie Organische Chemie NE 17 RU Newcastle Iiinrain 52a Universitatsstrasse 16 upon Thyne A-6020 Innsbruck CH-8092 Zurich This book was carefully produced. Nevertheless, authors, editor and publisher do not warrant the in- formation contained theirein to be free of errors. Readers are advised to keep in mind that statements, data, illustrations, procedural details or other items may inadvertently be inaccurate. L I The cover picture shows a cartoon of B,,-dependent methionine synthase (see contribution by Drennan et al. in this book). The picture was kindly provided by Martin Tollinger, University of Innsbruck. Library of Congress Card No.: applied for British Library Cataloguing-in-Publication Data: A catalogue record for this book is available from the British Library Die Deutsche Bibliothek - CIP-Einheitsaufnahme Vitamin B,, and B,,-proteins :lectures presented at the 4th European Symposium on Vitamin B,, and B,,-Proteins / ed.