For life science research only. Not for use in diagnostic procedures. DNA Molecular Weight Marker XVI (0.25–3.09 kbp) 250 base pair ladder

Cat. No. 11 855 638 001 y Version 06 ␮ c 50 g 1 A260 unit Content version: March 2017 50 ␮g (200 ␮l) for 50 gel lanes Store at Ϫ15 to Ϫ25°C

Product overview

Formulation Solution in 10 mM Tris-HCl, 1 mM EDTA, pH 8

Concentration 250 µg/ml

Size distribution Fragment mixture prepared by cleavage of specially con- structed plasmids with restriction endonucleases. The mixture contains 12 blunt-ended, double-stranded DNA fragments with the following base pair lengths: 250, 500, 750, 1000, 1250, 1500, 1750, 2000, 2250, 2500, 2750, 3000. The 1000 and 2000 bp banding pattern are 3 × brighter. Electrophoretic separation of this molecular weight marker results in a regular pattern.

Application The 250 bp ladder allows accurate sizing of DNA frag- ments generated by PCR or restriction digest separated on agarose gels. The DNA Molecular Weight Marker XVI can be used in conjunction with DNA Molecular Weight Marker XIII (50 bp ladder)*, DNA Molecular Weight Marker XIV (100 bp ladder)* and DNA Molecular Weight Marker XVII (500 bp ladder) * for precise size determi- nation..

Typical analysis The DNA fragment mixture shows a banding ladder with 12 DNA fragments in agarose gel electrophoresis as shown in the figure.

Separation Apply 1 µg DNA per lane on a 0.7% Agarose MP* gel. conditions

Storage/stability The unopened vial is stable at Ϫ15 to Ϫ25°C until the Fig.: Separation of 1 µg DNA Molecular Weight Marker XVI on a expiration date printed on the label 0.7 % agarose gel. Ethidium bromide stain.  This product is shipped in dry ice and should be Ϫ Ϫ stored at 15 to 25°C until use. Changes to Editorial Changes Once thawed we recommend further storage at +2 to previous version + 8°C. Repeated freezing and thawing should be avoided. Trademarks All third party product names and trademarks are the property of their respective owners.

* available from Roche Diagnostics Regulatory For life science research only. Not for use in diagnostic Disclaimer procedures.

Disclaimer of For patent license limitations for individual products License please refer to: List of biochemical reagent products

0317.11823 809001‘ sigma-aldrich.com Ordering Information

Product Application Packsize Cat. No. Agarose Gel For the elution of 1 Kit (max. 100 11 696 505 001 DNA Extraction DNA fragments from reactions) Kit agarose gels. High Pure PCR Purification of PCR or 50 purifications 11 732 668 001 Product Purifica- enzymatic modifica- 250 purifications 11 732 676 001 tion Kit tion reaction (e.g. restriction digest) SuRE/Cut Buf- Incubation buffers 1 ml each (10× 11 082 035 001 fer Set for A,B,L,M and H for conc. solutions) Restriction restriction enzymes Enzymes SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 959 001 fer A incubation conc. solution) SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 967 001 fer B incubation conc. solution) SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 991 001 fer H incubation conc. solution) SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 975 001 fer L incubation conc. solution) SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 983 001 fer M incubation conc. solution) Water, PCR Specially purified, 100 ml 03 315 843 001 Grade double-distilled, (4 vials of 25 ml) deionized, and auto- 25 ml 03 315 932 001 claved (25 vials of 1 ml) 25 ml 03 315 959 001 (1 vial of 25 ml) BSA, special Maintaining enzyme 20 mg (1 ml) 10 711 454 001 quality for stability molecular biology

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