bioRxiv preprint doi: https://doi.org/10.1101/155721; this version posted November 28, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Metabarcoding for the parallel identification of several hundred predators and their preys: application to bat species diet analysis MAXIME GALAN*1, JEAN-BAPTISTE PONS2, ORIANNE TOURNAYRE1, ÉRIC PIERRE1, MAXIME LEUCHTMANN3, DOMINIQUE PONTIER2.4.* and NATHALIE CHARBONNEL1* Full postal addresses 1CBGP, INRA, CIRAD, IRD, Montpellier SupAgro, Univ. Montpellier, 755 avenue du Campus Agropolis, CS 300 16, F-34988 Montferrier sur Lez cedex, France 2Univ Lyon, LabEx ECOFECT Ecoevolutionary Dynamics of Infectious Diseases, Villeurbanne F-69365 Lyon, France 3 Nature Environnement, 2 Avenue Saint-Pierre, 17700 Surgères, France 4Univ Lyon, Université Lyon 1, CNRS, Laboratoire de Biométrie et Biologie Évolutive UMR5558, F-69622 Villeurbanne, France * equal author contributions Keywords (4-6): Chiroptera, Arthropoda, environmental DNA (eDNA), high-throughput sequencing, false positives, predator–prey interactions Name, address, fax number and email of corresponding author * Maxime Galan, INRA, CBGP (INRA, CIRAD, IRD, Montpellier SupAgro, Univ. Montpellier), 755 avenue du Campus Agropolis, CS 300 16, F-34988 Montferrier sur Lez cedex, France. Fax: (+00 33) 04 99 62 33 05; E-mail:
[email protected] Running title: Metabarcoding of predators and their preys 1 bioRxiv preprint doi: https://doi.org/10.1101/155721; this version posted November 28, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.