DOCSLIB.ORG

ACTA BIOLÓGICA COLOMBIANA Acta Biol

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
ACTA BIOLÓGICA COLOMBIANA Acta Biol ACTA BIOLÓGICA COLOMBIANA Acta biol. Colomb. UNIVERSIDAD NACIONAL DE COLOMBIA FACULTAD DE CIENCIAS DEPARTAMENTO DE BIOLOGÍA Periodicidad Cuatrimestral VIII Simposio Colombiano y IV LAtinoamericano de Virologia, Universidad El Bosque, Bogota. Octubre 3 al 5 de 2019 ISSN impreso 0120-548X / ISSN online 1900-1649 Portada: Imagen diseñada por Vicky Constanza Roa Linares y Juan Carlos Gallego Gómez del Grupo Medicina Molecular y de Translación, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia ACTA BIOLÓGICA COLOMBIANA VOLUMEN 24(3) SEPTIEMBRE-DICIEMBRE 2019 Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología ISSN 0120-548X, ISSN online 1900-1649 / Fax: 316 53 10 / AA 14490 Bogotá D. C., Colombia. E-mail: [email protected] / http://www.revistas.unal.edu.co/index.php/actabiol Rectora: Dolly Montoya Castaño. Decano: Jairo Alexis Rodríguez López. Vicedecano de Investigación y Extensión:. William Javier Herrera. Director Departamento de Biología: José Ernesto Mancera Pineda. Editora Nubia Estela Matta Camacho, Ph.D. Departamento de Biología, Universidad Nacional de Colombia. Bogotá, Colombia. Comité Científico Carlos Castillo-Chavez, Simon A. Levin Mathematical, Computational & Modeling Sciences Center, Arizona State University, USA. Peter Adler, Ph.D. Entomology Program. School of Agricultural, Forest & Environmental Sciences, Clemson University. USA. Alda Maria Da-Cruz, Ph.D. Lab Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil. Rafael Lemaitre, Ph.D. Department of Invertebrate Zoology, National Museum of Natural History, Smithsonian Institution. Washington, USA. Claus Ramussen, Ph.D. Department of Bioscience- Genetics and Ecology, Aarhus University. Dinamarca. Reinhard Schnetter, Ph.D. Justus-Liebig-Universitat Giessen, Institut fur Allgemeine Botanik und Pflanzenphysiologie Bereich: Allgemeine Botanik. Giessen, Alemania. Walter Stuhmer, Ph.D. Abteilungen Molekulare Biologie Neuronaler Signale, Max-Plank-Institut Fur ExperimentelleMedizin, Gottingen, Alemania. Gediminas Valkiūnas, Ph.D. Center for Tropical Research, Institute of Ecology, Vilnius University, Lituania. Comité Editorial Adriano Gomes da Silva, Ph.D. Instituto Oswaldo da Cruz. Lab Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil. Alan Giraldo, Ph.D. Departamento de Biología, Universidad del Valle, Cali, Colombia. Allan H. Smith Pardo, Ph.D. National Apoidea Specialist USDA–APHIS–PPQ, Plant Inspection Station, California, USA. Argel Aguilar Valles, Ph.D. Department of Neuroscience, Carleton University, Ottawa, Canada. Artur Campos Dália Maia, Ph.D. Programa de Pós-graduação em Biologia Animal, Universidade Federal de Pernambuco, Brasil. Carolina Firacative, Ph.D. Escuela de Medicina y Ciencias de la Salud, Universidad del Rosario, Bogotá D.C., Colombia. Caroline Turchetto, Professor in Botany Departament, Universidade Federal do Rio Grande do Sul, Brasil. Diego Santiago Alarcón, Ph.D. Red de Biología y Conservación de Vertebrados, Instituto de Ecología, Xalapa, México. Edna Patricia Matta Camacho, Ph.D. Bioquímica y Estructura de Proteinas. Carleton University, Ottawa, Canadá. Enrique Arbeláez Cortés, Ph.D. Escuela de Biología, Facultad de Ciencias, Universidad Industrial de Santander, Bucaramanga, Colombia. Fernando Cárdenas P., Ph.D. Laboratorio de Neurociencia y Comportamiento, Universidad de los Andes, Bogotá D.C., Colombia. Francisco José Martínez Pérez, Ph.D. Escuela de Biología, Universidad Industrial de Santander, Colombia. Geraldo Andrade-Carvalho, Ph.D. Universidade Federal de Lavras, Departamento de Entomologia, Setor de Entomologia, Brasil. Geraldo Mäder, Ph.D. Pós-graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul, Brasil. Graciela Bailliet, Ph.D. Instituto Multidiciplinario de Biología Celular (IMBICE), Argentina. Héctor Jaime Gasca Alvarez, Ph. D. Corporación Sentido Natural, Bogotá, Colombia. Hernán Mauricio Romero, Ph.D. Departamento de Biología, Universidad Nacional de Colombia, Sede Bogotá. Bogotá D.C., Colombia. Howard Junca, Ph.D. Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Brunswick, Germany. John L Carr, Ph.D. Curator Museum of Natural History, Department of Biology University of Louisiana at Monroe, USA. José Rolando Bastida Zavala, Ph.D. Laboratorio de Sistemática de Invertebrados Marinos (LABSIM), Universidad del Mar, Campus Puerto Ángel, México. Juan F. González, Ph.D. Research Scientist, Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio, USA. Leonardo Miguel Galindo González, M.Sc. PhD. Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, Canada. Magdi T. Abdelhamid, Ph.D. National Research Centre, Botany Department, Cairo, Egypt. Manuel Alfonso Patarroyo, M.D., Ph.D. Departamento de Biología Molecular e Inmunología, Fundación Instituto de Inmunología de Colombia (FIDIC). Bogotá D.C., Colombia. María Isabel Arnaldos Sanabria, Ph.D. Departamento de Zoología y Antropología Física, Universidad de Murcia, España. Martha Patricia Ramírez Pinilla, Ph.D. Escuela de Biología, Universidad Industrial de Santander, Colombia. Mauricio Rodríguez Lanetty, Ph.D. Marine Sciences Program, School of Environment, Arts and Society, Florida International University, USA. Mónica Cadenazzi, Ph.D. Departamento de Biometría, Estadística y Computación, Universidad de la República Uruguay, Montevideo, Uruguay. Nataly Castelblanco, Ph.D. Consejo Nacional de Ciencia y Tecnología; Universidad de Quintana Roo, México. Nicolás Ubero Pascal, Ph.D. Departamento Zoología y Antropología Física, Universidad de Murcia, España. Nubia E. Matta Camacho, M.Sc., Ph.D. Departamento de Biología, Facultad de Ciencias, Universidad Nacional de Colombia. Bogotá D.C., Colombia. Omar Hernando Avila-Poveda, Ph.D. Facultad de Ciencias del Mar (FACIMAR), Universidad Autónoma de Sinaloa (UAS), Mazatlán, Sinaloa, México. Rafael Gutiérrez López, Ph.D. Estación Biológica de Doñana, Sevilla, España. Santiago Gaviria Melo, Ph.D. Department of Limnology and Bio-Oceanography, University of Vienna, Vienna, Austria. Sergi Sabater, Ph.D. Professor of Ecology Aquatic Ecology Institute, University of Girona, Spain and Catalan Institute for Water Research (ICRA), Girona, Spain. Sergio E. Bermúdez C, Ph.D. Instituto Conmemorativo Gorgas de Estudios de la Salud, Panamá. Sonia Antonieta Gallina Tessaro, Ph.D. Red de Biología y Conservación de Vertebrados, Instituto de Ecología, A.C., Xalapa, Veracruz, México. Susana Raquel Feldman, Ph.D. Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Argentina. Valeria Pizarro, Ph.D. CEI/PIMS Research Associate, Cape Eleuthera Research Institute, Eleuthera, Bahamas Víctor H. González, Ph.D. Division of Entomology, Natural History Museum, University of Kansas. Kansas, United States. Xavier Marquínez Casas, Ph.D. Departamento de Biología, Facultad de Ciencias, Universidad Nacional de Colombia. Bogotá D.C., Colombia. Facultad de Ciencias ACTA BIOLÓGICA COLOMBIANA Departamento de Biología http://www.revistas.unal.edu.co/index.php/actabiol Sede Bogotá RESÚMENES PONENCIAS DEL VIII SIMPOSIO COLOMBIANO Y IV CONGRESO LATINOAMERICANO DE VIROLOGÍA VIII Simposio Colombiano y IV Congreso Latinoamericano de Virología. Universidad El Bosque, Bogotá. Octubre 3, 4 y 5 de 2019 MG 01 USO DE LOS ROTAVIRUS COMO VIRUS ONCOLITICOS Carlos A. GUERRERO FONSECA1, Rafael A GUERRERO1, Luz Dary GUTIÉRREZ1, Henry SOSSA ROJAS1, Angie BEDOYA1, José RICO1, Claudia PÉREZ1, Juan Carlos HERNANDEZ1, Elver SILVA1, Orlando ACOSTA1 1Universidad Nacional de Colombia. Facultad de Medicina. Laboratorio de Biología Molecular de Virus [email protected] Introducción. Numerosos virus oncolíticos han completado ensayos clínicos con resultados satisfactorios para una gran variedad de tumores sólidos. Estos virus son derivados atenuados o modificados genéticamente y no afectan a las células sanas. Un inconveniente es la heterogeneidad genética observada en las diferentes líneas celulares de cáncer, entre tumores similares de diferentes individuos y aún entre células de un mismo tumor. El rotavirus (RV) un miembro de la familia Reoviridae, es responsable frecuente de gastroenteritis infantil. Nuestro grupo. ha asociado la presencia en la membrana citoplasmática de las proteínas PDI, la integrina v3 y la proteína de choque térmico Hsc70 como proteínas participantes durante el proceso infeccioso del RV en la célula diana. Estas tres proteínas se expresan en la membrana del enterocito, y no se expresan en conjunto en las células normales, excepto en las neoplasias. Esta condición puede favorecer la infección rotaviral en las células tumorales. Objetivo. Determinar la capacidad infecciosa de los aislamientos, los correceptores celulares en el proceso infeccioso y los nanocomplejos que permitan la llegada del virus al tumor eludiendo la respuesta anti-rotaviral. Metodología. Se evaluó la capacidad infecciosa de cinco aislamientos rotavirales correlacionando con la expresión de las proteínas de membrana citoplasmática: integrinas , PDIs y HSPs (Hsc70,Hsp90, Hsp70, Hsp60 y Hsp40) en las líneas celulares de cáncer: leucemia linfocítica aguda (no T; no B) Reh, cáncer de prostata PC3, gástrico NCI-N87, AGS y Kato III, cáncer de pulmón A549, cáncer de mama (MCF-7, MDA), cáncer de ovario MES-OV, melanoma A375 y SK MEL. Igualmente, en cultivo primario de leucemia linfoblástica aguda
Load more

Recommended publications
  • Kaistella Soli Sp. Nov., Isolated from Oil-Contaminated Soil
    A001 Kaistella soli sp. nov., Isolated from Oil-contaminated Soil Dhiraj Kumar Chaudhary1, Ram Hari Dahal2, Dong-Uk Kim3, and Yongseok Hong1* 1Department of Environmental Engineering, Korea University Sejong Campus, 2Department of Microbiology, School of Medicine, Kyungpook National University, 3Department of Biological Science, College of Science and Engineering, Sangji University A light yellow-colored, rod-shaped bacterial strain DKR-2T was isolated from oil-contaminated experimental soil. The strain was Gram-stain-negative, catalase and oxidase positive, and grew at temperature 10–35°C, at pH 6.0– 9.0, and at 0–1.5% (w/v) NaCl concentration. The phylogenetic analysis and 16S rRNA gene sequence analysis suggested that the strain DKR-2T was affiliated to the genus Kaistella, with the closest species being Kaistella haifensis H38T (97.6% sequence similarity). The chemotaxonomic profiles revealed the presence of phosphatidylethanolamine as the principal polar lipids;iso-C15:0, antiso-C15:0, and summed feature 9 (iso-C17:1 9c and/or C16:0 10-methyl) as the main fatty acids; and menaquinone-6 as a major menaquinone. The DNA G + C content was 39.5%. In addition, the average nucleotide identity (ANIu) and in silico DNA–DNA hybridization (dDDH) relatedness values between strain DKR-2T and phylogenically closest members were below the threshold values for species delineation. The polyphasic taxonomic features illustrated in this study clearly implied that strain DKR-2T represents a novel species in the genus Kaistella, for which the name Kaistella soli sp. nov. is proposed with the type strain DKR-2T (= KACC 22070T = NBRC 114725T). [This study was supported by Creative Challenge Research Foundation Support Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF- 2020R1I1A1A01071920).] A002 Chitinibacter bivalviorum sp.
    [Show full text]
  • Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies
    viruses Review Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies Varvara I. Maliogka 1,* ID , Angelantonio Minafra 2 ID , Pasquale Saldarelli 2, Ana B. Ruiz-García 3, Miroslav Glasa 4 ID , Nikolaos Katis 1 and Antonio Olmos 3 ID 1 Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; [email protected] 2 Istituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche, Via G. Amendola 122/D, 70126 Bari, Italy; [email protected] (A.M.); [email protected] (P.S.) 3 Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain; [email protected] (A.B.R.-G.); [email protected] (A.O.) 4 Institute of Virology, Biomedical Research Centre, Slovak Academy of Sciences, Dúbravská cesta 9, 84505 Bratislava, Slovak Republic; [email protected] * Correspondence: [email protected]; Tel.: +30-2310-998716 Received: 23 July 2018; Accepted: 13 August 2018; Published: 17 August 2018 Abstract: Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted through vegetative propagation and grafting of infected plant material. Some of these pathogens cause severe crop losses and often reduce the productive life of the orchards. Detection and characterization of these agents in fruit trees is challenging, however, during the last years, the wide application of high-throughput sequencing (HTS) technologies has significantly facilitated this task. In this review, we present recent advances in the discovery, detection, and characterization of fruit tree viruses and virus-like agents accomplished by HTS approaches.
    [Show full text]
  • Viruscollect Fulfilling the Need for a Common Reference Collection of Plant Viruses and Viroids
    Final Report VirusCollect Fulfilling the need for a common reference collection of plant viruses and viroids Project Duration: 2 years Start date: 2013/11/01 End date: 2015/11/01 Contents 1 Research consortium partners 3 2 Executive summary 5 3 Report 7 3.1 Introduction 7 3.2 Objectives and tasks of the project 9 3.3 Methods and results obtained 10 3.3.1 - Work package 1 Coordination of the VirusCollect project 11 3.3.2 - Work package 2 Identification of gaps in Q-bank 12 3.3.3 - Work package 3 Description of quality standards 13 3.3.4 - Work package 4 AGES 15 3.3.5 - Work package 5 DSMZ 16 3.3.6 - Work package 6 WUR 19 3.3.7 - Work package 7 SASA 23 3.3.8 - Work package 8 Data and isolates in Q-bank 30 4 Discussion of results and their reliability 31 5 Main conclusions 34 6 Dissemination activities 35 References 37 Annexes 39 1 Summary 1st year meeting at DSMZ 2014 39 2 Summary 2nd year and final meeting at NPPO-NL, 2015 42 3 Quality standards for Q-bank collections of viruses and viroids 45 VirusCollect Page 2 of 46 1. Research Consortium Partners Coordinator – Partner 1 Organisation NVWA (NPPO) Name of Contact Dr. J.W. Roenhorst Gender: F (incl. Title) Job Title Senior Scientist Plant Virology Postal Address P.O. Box 9102, 6700 HC Wageningen, the Netherlands E-mail [email protected] Phone +31 882232256 Partner 2 Organisation Wageningen University and Research Name of Contact Prof.
    [Show full text]
  • Identyfikacja Domen WG/GW Zaangażowanych W Wiązanie Białek Argonaute Oraz Analiza Mechanizmów Molekularnych Odpowiedzialnych Za Ich Zmienność
    Wydział Biologii Uniwersytet im. Adama Mickiewicza w Poznaniu Rozprawa doktorska Identyfikacja domen WG/GW zaangażowanych w wiązanie białek Argonaute oraz analiza mechanizmów molekularnych odpowiedzialnych za ich zmienność Andrzej Zieleziński Praca napisana pod kierunkiem Prof. dr. hab. Wojciecha Karłowskiego w Pracowni Bioinformatyki Instytutu Biologii Molekularnej i Biotechnologii Poznań, 2014 Podziękowania Składam serdeczne podziękowania mojemu Promotorowi Panu prof. dr. hab. Wojciechowi Karłowskiemu za intelektualne inspiracje oraz wiedzę, jaką przekazał mi w okresie studiów doktoranckich, a także twórcze rozwinięcie moich zainteresowań naukowych, tak abym mógł przygotować niniejszą dysertację Dziękuję również Koleżankom i Kolegom z Pracowni Bioinformatyki, w szczególności dr. Maciejowi Szymańskiemu, dr. Markowi Żywickiemu oraz mgr Sylwii Alabie za pomoc w kwestiach naukowych, a także pouczające dyskusje Finansowanie Niniejsza praca powstała przy finansowym udziale: 1. Narodowego Centrum Nauki (grant 2011/03/N/NZ2/01440 dla A.Z.) 2. Wydziału Biologii Uniwersytetu im. Adama Mickiewicza w Poznaniu (grant GDWB-09/2011, dla A.Z.) 3. Wojewódzkiego Urzędu Pracy (stypendium w ramach projektu „Wsparcie stypendialne dla doktorantów na kierunkach uznanych za strategiczne z punktu widzenia rozwoju Wielkopolski” w ramach programu 8.2.2. Programu Operacyjnego Kapitał Ludzki) Publikacje autora związane z pracą doktorską 1. Zielezinski A & Karlowski WM. Tyrosine-tryptophan substitution switches on the rapid evolution of WG/GW domain in RRM AGO-binding proteins from Arabidopsis and rice. [publikacja w przygotowaniu] 2. Zielezinski A & Karlowski WM. Whub: a comprehensive knowledgebase portal for AGO-binding protein research Nucleic Acids Research [artykuł wysłany do redakcji] 3. Zielezinski A & Karlowski WM. Agos - -a universal web tool for GW Argonaute-binding domain prediction. Bioinformatics. 27(9). 2011 4.
    [Show full text]
  • Evidence to Support Safe Return to Clinical Practice by Oral Health Professionals in Canada During the COVID-19 Pandemic: a Repo
    Evidence to support safe return to clinical practice by oral health professionals in Canada during the COVID-19 pandemic: A report prepared for the Office of the Chief Dental Officer of Canada. November 2020 update This evidence synthesis was prepared for the Office of the Chief Dental Officer, based on a comprehensive review under contract by the following: Paul Allison, Faculty of Dentistry, McGill University Raphael Freitas de Souza, Faculty of Dentistry, McGill University Lilian Aboud, Faculty of Dentistry, McGill University Martin Morris, Library, McGill University November 30th, 2020 1 Contents Page Introduction 3 Project goal and specific objectives 3 Methods used to identify and include relevant literature 4 Report structure 5 Summary of update report 5 Report results a) Which patients are at greater risk of the consequences of COVID-19 and so 7 consideration should be given to delaying elective in-person oral health care? b) What are the signs and symptoms of COVID-19 that oral health professionals 9 should screen for prior to providing in-person health care? c) What evidence exists to support patient scheduling, waiting and other non- treatment management measures for in-person oral health care? 10 d) What evidence exists to support the use of various forms of personal protective equipment (PPE) while providing in-person oral health care? 13 e) What evidence exists to support the decontamination and re-use of PPE? 15 f) What evidence exists concerning the provision of aerosol-generating 16 procedures (AGP) as part of in-person
    [Show full text]
  • Viroze Biljaka 2010
    VIROZE BILJAKA Ferenc Bagi Stevan Jasnić Dragana Budakov Univerzitet u Novom Sadu, Poljoprivredni fakultet Novi Sad, 2016 EDICIJA OSNOVNI UDŽBENIK Osnivač i izdavač edicije Univerzitet u Novom Sadu, Poljoprivredni fakultet Trg Dositeja Obradovića 8, 21000 Novi Sad Godina osnivanja 1954. Glavni i odgovorni urednik edicije Dr Nedeljko Tica, redovni profesor Dekan Poljoprivrednog fakulteta Članovi komisije za izdavačku delatnost Dr Ljiljana Nešić, vanredni profesor – predsednik Dr Branislav Vlahović, redovni profesor – član Dr Milica Rajić, redovni profesor – član Dr Nada Plavša, vanredni profesor – član Autori dr Ferenc Bagi, vanredni profesor dr Stevan Jasnić, redovni profesor dr Dragana Budakov, docent Glavni i odgovorni urednik Dr Nedeljko Tica, redovni profesor Dekan Poljoprivrednog fakulteta u Novom Sadu Urednik Dr Vera Stojšin, redovni profesor Direktor departmana za fitomedicinu i zaštitu životne sredine Recenzenti Dr Vera Stojšin, redovni profesor, Univerzitet u Novom Sadu, Poljoprivredni fakultet Dr Mira Starović, naučni savetnik, Institut za zaštitu bilja i životnu sredinu, Beograd Grafički dizajn korice Lea Bagi Izdavač Univerzitet u Novom Sadu, Poljoprivredni fakultet, Novi Sad Zabranjeno preštampavanje i fotokopiranje. Sva prava zadržava izdavač. ISBN 978-86-7520-372-8 Štampanje ovog udžbenika odobrilo je Nastavno-naučno veće Poljoprivrednog fakulteta u Novom Sadu na sednici od 11. 07. 2016.godine. Broj odluke 1000/0102-797/9/1 Tiraž: 20 Mesto i godina štampanja: Novi Sad, 2016. CIP - Ʉɚɬɚɥɨɝɢɡɚɰɢʁɚɭɩɭɛɥɢɤɚɰɢʁɢ ȻɢɛɥɢɨɬɟɤɚɆɚɬɢɰɟɫɪɩɫɤɟɇɨɜɢɋɚɞ
    [Show full text]
  • Molecular Characterization of an Unusual New Plant RNA Virus Reveals an Evolutionary Link Between Two Different Virus Families
    RESEARCH ARTICLE Molecular characterization of an unusual new plant RNA virus reveals an evolutionary link between two different virus families 1 1 2 2 Sun-Jung Kwon , Gug-Seoun Choi , Boram Choi , Jang-Kyun SeoID * 1 Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Wanju, Republic of Korea, 2 Department of International Agricultural Technology and Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang, Republic of Korea * [email protected] a1111111111 a1111111111 a1111111111 a1111111111 Abstract a1111111111 An unusual novel plant virus provisionally named goji berry chlorosis virus (GBCV) was iso- lated from goji berry plants (Lycium chinense Miller) showing chlorosis symptoms and its complete genome sequence was determined. The viral genome consists of a positive- sense single-stranded RNA of 10,100 ribonucleotides and contains six open reading frames OPEN ACCESS (ORFs). Electron microscopy showed that the viral genome is packaged as a filamentous Citation: Kwon S-J, Choi G-S, Choi B, Seo J-K particle with an average length of approximately 850 nm. Phylogenetic analysis and amino (2018) Molecular characterization of an unusual acid similarity analysis of the encoded ORFs revealed that this new virus could be classified new plant RNA virus reveals an evolutionary link between two different virus families. PLoS ONE 13 in an intermediate position between the families Benyviridae and Virgaviridae. The GBCV (10): e0206382. https://doi.org/10.1371/journal. 200-kDa replicase (ORF1) is more similar to benyvirus replicases than to virgavirus repli- pone.0206382 cases, while its 17-kDa coat protein (CP, ORF2) is more closely related with virgavirus CPs Editor: A.
    [Show full text]
  • Evidence to Support Safe Return to Clinical Practice by Oral Health Professionals in Canada During the COVID- 19 Pandemic: A
    Evidence to support safe return to clinical practice by oral health professionals in Canada during the COVID- 19 pandemic: A report prepared for the Office of the Chief Dental Officer of Canada. March 2021 update This evidence synthesis was prepared for the Office of the Chief Dental Officer, based on a comprehensive review under contract by the following: Raphael Freitas de Souza, Faculty of Dentistry, McGill University Paul Allison, Faculty of Dentistry, McGill University Lilian Aboud, Faculty of Dentistry, McGill University Martin Morris, Library, McGill University March 31, 2021 1 Contents Evidence to support safe return to clinical practice by oral health professionals in Canada during the COVID-19 pandemic: A report prepared for the Office of the Chief Dental Officer of Canada. .................................................................................................................................. 1 Foreword to the second update ............................................................................................. 4 Introduction ............................................................................................................................. 5 Project goal............................................................................................................................. 5 Specific objectives .................................................................................................................. 6 Methods used to identify and include relevant literature ......................................................
    [Show full text]
  • Deep Sequencing of Banana Bract Mosaic Virus from flowering Ginger (Alpinia Purpurata) and Development of an Immunocapture RT-LAMP Detection Assay
    Arch Virol DOI 10.1007/s00705-016-2830-y ORIGINAL ARTICLE Deep sequencing of banana bract mosaic virus from flowering ginger (Alpinia purpurata) and development of an immunocapture RT-LAMP detection assay 1,2 1 2 1 Jingxin Zhang • Wayne B. Borth • Birun Lin • Kishore K. Dey • 1 2 2 2 Michael J. Melzer • Huifang Shen • Xiaoming Pu • Dayuan Sun • John S. Hu1 Received: 9 October 2015 / Accepted: 14 March 2016 Ó Springer-Verlag Wien 2016 Abstract Banana bract mosaic virus (BBrMV) has never hypothesis that the A. purpurata isolate arrived in Hawaii been reported in banana plants in Hawaii. In 2010, how- from Southeast Asia. ever, it was detected in a new host, flowering ginger (Alpinia purpurata). In this study, we characterize the A. purpurata isolate and study its spread in flowering ginger Introduction in Hawaii. A laboratory study demonstrated that BBrMV could be transmitted from flowering ginger to its natural Banana bract mosaic virus (BBrMV) was first isolated from host, banana, therefore raising a serious concern about the bananas (Musa spp. L., Musaceae, Zingiberales) in the potential risk to the rapidly growing banana industry of Philippines in 1979 [1]. It has subsequently been detected Hawaii. To quickly monitor this virus in the field, we in other countries including India, Samoa, Sri Lanka, developed a robust immunocapture reverse transcription Thailand, and Vietnam [2]. Recently, the virus was dis- loop-mediated isothermal amplification (IC-RT-LAMP) covered in Colombia and Ecuador [3], indicating that this assay. Deep sequencing of the BBrMV isolate from A. pathogen can spread quickly and cause significant eco- purpurata revealed a single-stranded RNA virus with a nomic losses worldwide.
    [Show full text]
  • Plant Virus RNA Replication
    eLS Plant Virus RNA Replication Alberto Carbonell*, Juan Antonio García, Carmen Simón-Mateo and Carmen Hernández *Corresponding author: Alberto Carbonell ([email protected]) A22338 Author Names and Affiliations Alberto Carbonell, Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Campus UPV, Valencia, Spain Juan Antonio García, Centro Nacional de Biotecnología (CSIC), Madrid, Spain Carmen Simón-Mateo, Centro Nacional de Biotecnología (CSIC), Madrid, Spain Carmen Hernández, Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Campus UPV, Valencia, Spain *Advanced article Article Contents • Introduction • Replication cycles and sites of replication of plant RNA viruses • Structure and dynamics of viral replication complexes • Viral proteins involved in plant virus RNA replication • Host proteins involved in plant virus RNA replication • Functions of viral RNA in genome replication • Concluding remarks Abstract Plant RNA viruses are obligate intracellular parasites with single-stranded (ss) or double- stranded RNA genome(s) generally encapsidated but rarely enveloped. For viruses with ssRNA genomes, the polarity of the infectious RNA (positive or negative) and the presence of one or more genomic RNA segments are the features that mostly determine the molecular mechanisms governing the replication process. RNA viruses cannot penetrate plant cell walls unaided, and must enter the cellular cytoplasm through mechanically-induced wounds or assisted by a 1 biological vector. After desencapsidation, their genome remains in the cytoplasm where it is translated, replicated, and encapsidated in a coupled manner. Replication occurs in large viral replication complexes (VRCs), tethered to modified membranes of cellular organelles and composed by the viral RNA templates and by viral and host proteins.
    [Show full text]
  • A New Virus Found in Garlic Virus Complex Is a Member of Possible Novel Genus of the Family Betaflexiviridae (Order Tymovirales)
    A new virus found in garlic virus complex is a member of possible novel genus of the family Betaflexiviridae (order Tymovirales) Leonardo A. Da Silva1,*, Athos S. Oliveira1,*, Fernando L. Melo1, Daniel M.P. Ardisson-Araújo2, Francisco V. Resende3, Renato O. Resende1 and Bergmann M. Ribeiro1 1 Department of Cell Biology/Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, Brazil 2 Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil 3 Embrapa Hortalicas,¸ Empresa Brasileira de Pesquisa Agropecuária, Brasilia, Distrito Federal, Brazil * These authors contributed equally to this work. ABSTRACT Plant vegetative propagation strategies for agricultural crops cause the accumulation of viruses, resulting in the formation of virus complexes or communities. The cultivation of garlic is based on vegetative propagation and more than 13 virus species from the genera Potyvirus, Allexivirus and Carlavirus have been reported. Aiming for an unbiased identification of viruses from a garlic germplasm collection in Brazil, total RNA from eight garlic cultivars was sequenced by high-throughput sequencing (HTS) technology. Although most viruses found in this study were previously reported, one of them did not belong to any known genera. This putative new virus was found in seven out of eight garlic cultivars and phylogenetic data positioned it as representative of an independent evolutionary lineage within family Betaflexiviridae. This virus has been tentatively named garlic yellow mosaic-associated virus (GYMaV), sharing highest nucleotide identities with African oil palm ringspot virus (genus Robigovirus) and Submitted 31 July 2018 Accepted 14 December 2018 potato virus T (genus Tepovirus) for the replicase gene, and with viruses classified within Published 16 January 2019 genus Foveavirus for the coat protein gene.
    [Show full text]
  • Analysis of the Genetic Diversity of Grapevine Rupestris Stem Pitting-Associated Virus in Ontarian Vineyards and Construction of a Full-Length Infectious Clone
    Analysis of the genetic diversity of Grapevine rupestris stem pitting-associated virus in Ontarian vineyards and construction of a full-length infectious clone by Julia C. Hooker A thesis presented to The University of Guelph In partial fulfilment of requirements for the degree of Master of Science in Molecular and Cellular Biology ©Julia Hooker, April, 2017 ABSTRACT Analysis of the genetic diversity of Grapevine rupestris stem pitting-associated virus in Ontarian vineyards and construction of a full-length infectious clone Julia Catherine Hooker Advisor: University of Guelph, 2017 Dr. Baozhong Meng Advisory Committee: Dr. Peter Krell Dr. Annette Nassuth Grapevine rupestris stem pitting-associated virus (GRSPaV; Betaflexiviridae, Foveavirus) has been associated with a number of diseases including Syrah decline. Previous findings show GRSPaV sequence variants cluster into three or more main phylogroups, regardless of geographical location. Here, the genetic diversity of GRSPaV isolates from Ontarian vineyards was analyzed using broad-spectrum primers targeting the viral polymerase coding sequence. It was hypothesized that GRSPaV variants in Ontario are diverse and GRSPaV-SY variants are involved in Syrah decline symptoms. In total, 169 cDNA clones from 21 Vitis sources were used for phylogenetic analysis. Similarly to previous reports, four major lineages were observed; GRSPaV-PN, -SG1, -SY, and –GG. Variants of the GRSPaV–SY lineage were confirmed in all 14 sources tested with SY-specific primers. Syrah cultivars expressing red canopy decline symptoms had more clones clustering with the GRSPaV-SY lineage than those without observable decline symptoms. GRSPaV clones from 8 hybrid sources mostly clustered with GRSPaV-SY, followed distantly by GRSPaV-BS.
    [Show full text]