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Overview of Dendritic Cell Development

Lineage–, CD45+, Common CD117 (c-kit) Common MHCII+, CD11c+ – + CD207 (Langerin) Myeloid CD117 (c-kit) Lineage , CD45 , Myeloid Progenitor MHCII (HLA-DR)+, CD11c+ Progenitor CD324 (E-Cadherin) Human Mouse CD326 (EpCAM) CD207 (Langerin) TGFb1 Cells CD11b, CD115 Cells CD14 Monocyte CD324 (E-Cadherin) Monocyte M-CSF CD11b – + Ly6C Langerhans CD24 Lineage , CD45 , M-CSF CD326 (EpCAM) MHCII (HLA-DR)+, CD11c+ Langerhans CD11blo Zbtb46– Cells CD172a (Sirp-α) CD16 CD1ahi, CD1c CD205 (DEC-205) Cells CSF F4/80 CD64 CD172a (Sirp-α) Lineage–, CD45+, FLT3L TLR3, TLR11 CD1a, CD1c Inﬂammatory CD369 (Dectin-1/CLEC7A) MHCII+, CD11c+ +/– CSF IL-15 CD8–, CD14– CD11b, CD14 CD371 (CLEC12A) CD64 Monocyte- FLT3L Inﬂammatory CD370 (Clec9a)– CD172a (Sirp-α) IL-15 CLEC6A CD11b derived lo Monocyte- CD206, CD209 (DC-SIGN) TLR1, TLR2, TLR3 , TLR6 CD209a (DC-SIGN) CD367 (DCIR/CLEC4A) DCs CD14– CD272 (BTLA)lo derived CD369 (Dectin-1/CLEC7A) DCs Common Ly-6C – + CD371 (CLEC12A) CD117 (c-kit) Lineage , CD45 , IL-1β, IL-6, IL-10, TLR1-6, TLR7-8, TLR10 Dendritic + lo CLEC6A – – CD135/FLT3 MHCII , CD11c IL-12, IL-23, TNF CD8a , CD14 IL-1β, IL-6 IL-10, Precursor TLR3lo, TLR4, TLR7, TLR8 CD45R (B220) IL-12, IL-23, TNF Plasmacytoid CD207 (Langerin)– Cells CD317 (BST-2) Common Lineage–, CD45+, FLT3L DCs Lineage–, CD45+, + Ly6C + lo/– CD207 IFN Type I + + Dendritic CD135/FLT3 MHCII (HLA-DR) , CD11c Lineage–, CD45+, IRF7, IRF8, BATF3hi Siglec-H MHCII (HLA-DR) , CD11c hi – + CD123 + + Dermal SpiB, Zbtb46 CD1a, CD64 CD1a Precursor CD117 (c-kit) MHCII , CD11c CD4 CD303 FLT3L CD11b Dermal Cells CD207 (Langerin) DCs CD172a (Sirp-α) CD304 lo CD1c CD11b IFN-I, IFN-III (IFN-λ) CD209a (DC-SIGN) DCs CD85g (ILT7) – CD172a (Sirp-α) IL-3 Plasmacytoid CD172a (Sirp-α) IL-6, IL-8 CD272 (BTLA) CD4 CD367 (DCIR/CLEC4A) DCs IP-10 (CXCL10) CD370 (Clec9a) CD45RA hi hi CD369 (Dectin-1/CLEC7A) Lineage–, CD45+, TNF TLR7 , TLR9 IRF7, IRF8, CD272 (BTLA) Pre- CD11b– CLEC6A MHCII+, CD11c+ SpiB CD367 (DCIR/CLEC4A) CD207– – TLR1–3, TLR6, TLR7, TLR10 ± Conventional CD14 CD371 (CLEC12A)± CD11b CD14– Dermal Dendritic IFN-I, IFN-III (IFN-λ) TLR7hi, TLR9hi CD172a (Sirp-α) CD207 (Langerin)– – IL-6, IL-8 CD11b– CD207 (Langerin) DCs Cell CD209 (DC-SIGN)– IP-10 (CXCL10) CD14– Lineage–, CD45+, CD324 (E-Cadherin)– TNF + MHCII+, CD11chi CD326 (EpCAM)– CD103 CD103 Conventional CD205 (DEC-205) Lineage–, CD45+, Migratory CD207 (Langerin) + + Lineage–, CD45+, MHCII (HLA-DR) , CD11c Lineage–, CD45+, + hi – + + +/lo CD11b DCs CD272 (BTLA) CD14 Lineage , CD45 , MHCII (HLA-DR) , CD11c + + MHCII , CD11c IRF8, BATF3, CD370 (Clec9a) CD209 (DC-SIGN) MHCII (HLA-DR)+, CD11c+ CD141hi Conventional CD11b Lineage–, CD45+, CD8a+ Lineage–, CD45+, Zbtb46 NECL2 CD11b CD1c CD370 (CLEC9A) Migratory + hi + hi + CD172a (Sirp-α) CD4+ MHCII , CD11c MHCII , CD11c TLR3, TLR4, TLR11 CD1c CD14+ CD1c CD172a (Sirp-α) NECL2 CD209a (DC-SIGN)± DCs Conventional IFN-I, IFN-III (IFN-λ) + lo CD4 CD8a XCR1 CD172a (Sirp-α) CLEC6A CD11b lo Conventional Conventional CD141+ CD272 (BTLA) IRF4, IRF2 CD11b Lymphoid CD205 (DEC-205) IL-12 CD4–, CD8a– Dermal CD11b+/lo CD205 (DEC-205)hi hi CD367 (DCIR/CLEC4A) F4/80 Zbtb46, BATF3 Lymphoid +/– CXCL-10 (IP-10) – – Myeloid DCs hi CD172a (Sirp-α) Resident DCs CD207 (Langerin) CD11b , CD14 CD369 (Dectin-1/CLEC7A) DCs CX3CR1 Conventional CD272 (BTLA) TNF TLR5, TLR7, TLR9 IL-1β, IL-6, IL-8 Resident DCs CD205 (DEC-205) IRF8, BATF3, CD272 (BTLA)hi F4/80– IRF4 CD272(BTLA) CD366 (TIM-3) TLR1–3, TLR6, TLR7 Myeloid DCs CX3CR1 IRF4, Zbtb46 lo Zbtb46 – – IL-10, IL-12 CD272 (BTLA) CD370 (Clec9a) CX3CR1 CD1a CD366 (TIM-3) CD367 (DCIR/CLEC4A) – – hi IRF8, BATF3 CD4 , CD8a IL-23 BATF3 ESAM NECL2 – IFN-I, IFN-III (IFN-λ) CD207 (Langerin) CD367 (DCIR/CLEC4A) CD369 (Dectin-1/CLEC7A) CD14– – IL-1β, IL-6, IL-8 TNF F4/80 TLR3, TLR4, TLR11 CD324 (E-Cadherin) CD369 (CLEC7A) CD371 (CLEC12A) – IL-1β, IL-6, IL-8 IL-12 IL-10, IL-12 XCR1 TLR5, TLR7, TLR9 XCR1 CD326 (EpCAM)– CD371 (CLEC12A) IFN-I, IFN-III (IFN-λ) TLR3, TLR8 IL-10, IL-12 CXCL-10 (IP-10) CX3CR1 CD4– IL-23 TLR3lo, TLR4lo, TLR8, TLR10lo CXCL-10 (IP-10) XCR1 IL-23 TNF CD8a– CD11b– TNF CD1a–, CD14lo/– TNF CD1a–, CD14– TNF CD14– CD14–

For Research Use Only. Not for use in diagnostic or therapeutic procedures. 23-19088-00 Core dendritic cell phenotyping markers Secreted cytokines – – – – 1 Lineage (Hu): CD3 , CD19 , CD20 , CD56 , CD14 Transcription factors Lineage (Ms): CD3–, CD19–, CD49b– or NK1.1–, CD14– BD Life Sciences, San Jose, CA, 95131, USA Markers on this schematic can be altered as a result of cellular Dendritic cell subset markers (positive) environment, differentiation state and other factors. DC KEY TLR7, TLR8 and TLR9 are endosomal and require biomarker profiles were obtained from RNA and protein level intracellular staining analysis provided in peer reviewed publications and public bdbiosciences.com Dendritic cell subset markers (negative) databases. Visit bdbiosciences.com/DC for more information.