Herpetology Notes, volume 12: 975-980 (2019) (published online on 10 October 2019)

First record of Rhabdias cf. breviensis (Rhabditoidea: Rhabdiasidae) parasitizing acuminatus (Anura: ) in the Southern Pantanal wetland,

Isabela Caroline Oliveira da Silva1,*, Priscilla Soares1,2, Maiara Cabrera Miguel1,2, Rodney Murillo Peixoto Couto2, Giovana Martins Miranda1, André Mota Alves3, and Fernando Paiva1

Parasitic nematodes of the genus Rhabdias Stiles There are also some taxa only at generic level, or needing et Hassall, 1905 are lungworms commonly found in more information to confirm the specific identification: and some reptiles (Kuzmin et al., 2016). Rhabdias sp., Rhabdias aff. sphaerocephala, Rhabdias These helminths have a complex life cycle that cf. breviensis, and Rhabdias [cf. elegans] (Kloss 1971; alternates from a free-living generation on the soil to Sueldo and Ramirez, 1976; Baker, 1987; Vicente et al., another that is a parasite in the lung of amphibians and 1991; González and Hamann, 2007; Santos et al., 2011; reptiles (Baker, 1979; Langford and Janovy, 2009). In Hamann et al., 2013; Nascimento et al., 2013; Draghi South America, 12 species of Rhabdias were reported et al., 2015; Kuzmin et al., 2016; Teles et al., 2018a; parasitizing amphibians: R. androgyna Kloss, 1971; Oliveira et al., 2019). Those taxa are distributed in R. breviensis Nascimento et al., 2013; R. elegans , , , , Uruguay, and Brazil Gutiérrez, 1945; R. fuelleborni Travassos, 1926; R. (González and Hamann, 2007; McAllister et al., 2010; galactonoti Kuzmin et al., 2016; R. hermafrodita Kloss, Toledo et al., 2017). In the latter country, those species 1971; R. mucronata Schuurmans-Stekhoven, 1952; R. were reported to occur in the states of Amazonas, Bahia, paraensis Santos et al., 2011; R. pseudosphaerocephala Ceará, Distrito Federal, Espírito Santo, Mato Grosso do Kuzmin et al., 2007; R. sphaerocephala Goodey, 1924; Sul, Minas Gerais, Pará, Paraná, Pernambuco, Piauí, R. stenocephala Kuzmin et al., 2016; and R. truncata Rio de Janeiro, Rio Grande do Sul, and São Paulo Schuurmans-Stekhoven, 1952. However, R. mucronata (Kloss, 1971; Vicente et al., 1991; Souza et al., 2016; and R. truncata are species inquirendae that are poorly Müller et al., 2018). described and difficult to recognize, especially since The Mato Grosso snouted treefrog, Scinax acuminatus only female and young individuals are known (Barrella (Cope, 1862) (Figure 1), is a medium-sized, arboreal et al., 2010).

1 Laboratório de Parasitologia , Instituto de Biociências, Universidade Federal de Mato Grosso do Sul, Avenida Costa e Silva s/n, Campo Grande, Mato Grosso do Sul 79070-900, Brazil. 2 Laboratório de Pesquisa em Herpetologia, Instituto de Biociências, Universidade Federal de Mato Grosso do Sul, Avenida Costa e Silva s/n, Campo Grande, Mato Grosso do Sul 79070-900, Brazil. 3 Laboratório de Biologia Tropical, Instituto de Tecnologia e Pesquisa, Universidade Tiradentes, Avenida Murilo Dantas, 300, Farolândia, Aracaju, Sergipe 49032-490, Brazil. Figure 1. Specimen of Scinax acuminatus. Photo by Rodney * Corresponding author. E-mail: [email protected] Murillo Peixoto Couto. 976 Isabela Caroline Oliveira da Silva et al. and nocturnal hylid distributed in Argentina, , surrounded by two lateral lips and four submedian lips. Paraguay and in Brazil on the West and Midwest Morphological dimensions are provided in Table 2. regions (Frost, 2019). It is found in open areas where Although these characteristics confirm the similarity males vocalize perched on shrubs and branches at the with R. breviensis, we decided to identify as Rhabdias edge of permanent and semi-permanent (Prado et cf. breviensis, due to the genus Rhabdias present al., 2005; Prado and Haddad, 2005). Previous records of species with very similar morphological characteristics, parasites in this host show occurrences of acanthocephala mainly when it comes to female individuals as found Acanthocephalus caspanensis Fernandez and Ibarra, (Martínez-Salazar et al., 2009). Further research using 1989 and nematodes such as Aplectana sp. Railliet more specimens of this parasite are needed for specific and Henry, 1916; Cosmocerca parva Travassos, 1925; confirmation. Oxyascaris caudacutus (Freitas, 1958) Baker and Vaucher, 1984; Physaloptera Rudolphi, 1819; and Rhabdochonidae Skrjabin, 1946, in Argentina, Brazil and Paraguay (Vicente et al., 1991; Smales, 2007; González and Hamann, 2008, 2010). In this context the aim of this study was present the first record of Rhabdias cf. breviensis parasitizing S. acuminatus in southern Pantanal wetland, Mato Grosso do Sul State, Brazil. In December 2014, 15 specimens of S. acuminatus were collected in temporary and permanent ponds at the “Fazenda Nhumirim” (Nhecolândia sub-region), Municipality of Corumbá, Mato Grosso do Sul State, Brazil (-18.988054°, -56.619056°, datum: WGS84). Collected specimens were transported alive to the laboratory, and euthanasia was performed with the topical use of Xylocaine® ointment 5% (CONCEA, 2013). Subsequently, the body cavities and internal organs were examined for parasites. The parasite was found in the host lung, and after its removal prepared on a temporary slide with glycerine clarification for a morphological identification. Morphological and morphometric characters were taken under a light microscope LEICA model DM5500™, equipped with computerized system for capturing and processing images (LAS 3.9™ LEICA). The measures for morphological identification were performed in micrometres. The examined anuran specimen was fixed in formalin 10%, and preserved in alcohol 70o GL. Anuran and parasite specimen were housed at the Zoology Reference Collection of the Universidade Federal de Mato Grosso do Sul (Scinax acuminatus: ZUFMS-AMP12432, Rhabdias cf. breviensis: ZUFMS- NEM00074). The identified parasite is an adult hermaphrodite female that differs morphologically from other Rhabdias species due to reduced body size, along with the characteristics Figure 2. Rhabdias cf. breviensis recovered in an adult male of cuticular swelling covering the body extending, from Scinax acuminatus. (A). Light microscopy image, side view the buccal opening to the posterior end of the tail (Fig. of the body. (B). Schematic drawing; a = total body view; b = 2); two pairs of submedian cephalic papillae and the one extremity anterior of the body; c = extremity posterior of the pair of lateral cephalic papillae. It also has oral aperture body. Photo by Priscilla Soares. First record of Rhabdias cf. breviensis parasitizing Scinax acuminatus, Brazil 977

Table 1. Records of species of Rhabdias in South American anurans published between 2015 and 2019. Abbreviations for all countriesTable 1. citedRecords are of species in parentheses: of Rhabdias in ArgentinaSouth American (ARG); anurans published Brazil between(BRA); 2015 Peru and 2019.(PER). Abbreviations for all countries cited are in parentheses: Argentina (ARG); Brazil (BRA); Peru (PER).

Host (family/species) Species of Rhabdias Locality (Country) Reference Bufonidae diptycha (Cope, 1862) Rhabdias breviensis Municipality of Castilho, São Paulo State (BRA) Müller et al. (2018) Rhabdias pseudosphaerocephala Municipality of Castilho, São Paulo State (BRA) Müller et al. (2018) Municipality of Porto Rico, Paraná State (BRA) Müller et al. (2018) Rhabdias sp. Municipality of Botucatu, São Paulo State (BRA) Müller et al. (2018) (Spix, 1824) Rhabdias androgyna Municipality of Aiuaba, Ceará State (BRA) Teles et al. (2018a) Rhabdias breviensis Municipality of Picus, Piauí State (BRA) Müller et al. (2018) (Spix, 1824) Rhabdias fuelleborni Municipality of Botucatu, São Paulo State (BRA) Moretti et al. (2017) (Stevaux, 2002) Rhabdias pseudosphaerocephala Municipalities of Santana do Cariri and Farias Müller et al. (2018) Brito, Ceará State (BRA) Municipality of Picus, Piauí State (BRA) Müller et al. (2018) Rhinella major (Müller and Hellmich, 1936) Rhabdias elegans Chaco Province (ARG) Hamann and González (2017) Rhabdias pseudosphaerocephala Chaco Province (ARG) Hamann and González (2017) Rhinella marina (Linnaeus, 1758) Municipality of Manaus, Amazonas State (BRA) Müller et al. (2018) Municipality of Tarapoto, San Martín Department Toledo et al. (2017) (PER) Dendrobatidae Adelphobates galactonotus (Steindachner, 1864) Rhabdias galactonoti Municipality of Melgaço, Pará State (BRA) Kuzmin et al. (2016) Hylidae Boana raniceps (Cope, 1862) Rhabdias pseudosphaerocephala Municipality of Porto Rico, Paraná State (BRA) Müller et al. (2018) Rhabdias sp. Municipality of Selvíria, Mato Grosso do Sul State Souza et al. (2016) (BRA) Scinax fuscovarius (Lutz, 1925) Rhabdias breviensis Municipality of Castilho, São Paulo State (BRA) Müller et al. (2018) Rhabdias sp. Municipality of Diamante do Norte, Paraná State da Graça et al. (2017) (BRA) fuscus (Schneider, 1799) Rhabdias breviensis Municipality of Castilho,Barro, Ceará São StatePaulo (BRA) State (BRA) Müller et al. (2018) Leptodactylus macrosternum (Miranda-Ribeiro, Rhabdias cf. stenocephala Municipalities of Barro and Farias Brito, Ceará Müller et al. (2018) 1926) State (BRA) Rhabdias sp. Municipality of Aiuaba, Ceará State (BRA) Teles et al. (2018b) Leptodactylus pentadactylus (Laurenti, 1768) Rhabdias stenocephala Municipality of Melgaço, Pará State (BRA) Kuzmin et al. (2016) Rhabdias sp. Municipality of Corrientes, Corrientes Province González and Hamann (2016) (ARG) Leptodactylus podicipinus (Cope, 1862) Rhabdias breviensis Municipality of Castilho, São Paulo State (BRA) Müller et al. (2018) Leptodactylus vastus (Lutz, 1930) Rhabdias cf. stenocephala Municipalities of Aiuaba, Barro and Farias Brito Müller et al. (2018) Ceará State (BRA) Physalaemus albifrons (Spix, 1824) Rhabdias cf. breviensis Municipality of Farias Brito, Ceará State (BRA) Oliveira et al. (2019) Physalaemus cuvieri (Fitzinger, 1826) Rhabdias sp. Municipalities of São Luis do Paraitinga, Cunha, Aguiar et al. (2015) Ubatuba, and Natividade da Serra, São Paulo State (BRA) Municipality of Campina Grande do Sul, Paraná Leivas et al. (2018) State (BRA) Pseudopaludicola pocoto (Magalhães et al., 2014) Municipality of Aiuaba, Ceará State (BRA) Silva et al. (2018) Odontophrynidae Odontophrynus americanus (Duméril and Rhabdias sp. (cf. elegans) Municipality of Bonito, Mato Grosso do Sul State Silva et al. (2018) Bibron, 1841) (BRA) Proceratophrys aridus (Cruz et al., 2012) Rhabdias breviensis Municipality of Aiuaba, Ceará State (BRA) Müller et al. (2018) Phlyllomedusidae Pithecopus nordestinus Caramaschi, 2006 Rhabdias sp. Municipality of Jardim, Ceará State (BRA) Sena et al. (2018)

Rhabdias breviensis has been previously recorded in et al., 2013; Müller et al., 2018). Oliveira et al. (2019) eight anuran species: Leptodactylus fuscus (Schneider, found Rhabdias cf. breviensis parasitizing Physalaemus 1799); L. macrosternum (Miranda-Ribeiro, 1926); L. albifrons from the Municipality of Farias Brito, Ceará petersii (Steindachner, 1864); L. podicipinus (Cope, State. Therefore, we report the first occurrence of 1862); Proceratophrys aridus (Cruz et al., 2012); Rhabdias cf. breviensis parasitizing S. acuminatus in (Cope, 1862); R. granulosa (Spix, southern Pantanal wetland, Mato Grosso do Sul State, 1824) and Scinax fuscovarius (Lutz, 1925) (Nascimento Brazil. 978 Isabela Caroline Oliveira da Silva et al.

Table 2. A comparison of the morphometrics of Rhabdias breviensis collected in Pará State, with a female Rhabdias cf. breviensis collected inTable Mato 2. GrossoA comparison do Sul of theState, morphometrics Brazil. Measurements of Rhabdias breviensis are in collected micrometres in Pará State, (μm). with a female Rhabdias cf. breviensis collected in Mato Grosso do Sul State, Brazil. Measurements are in micrometres (µm).

Corumbá Municipality, Mato Grosso Breves Municipality, Marajó Island, do Sul State, Brazil (Present study) Pará State, Brazil (Nascimento et al., 2013) Host(s) Scinax acuminatus Leptodactylus petersii and L. macrosternum Characters An individual Type series Minimum – maximum (mean ± standard deviation) Total length 4.038,057 2.63–3.63 (3.14 ± 0.34) Greatest width 464,697 370–543 (423 ± 59) Buccal capsule (length x width) 9,135 × 12,555 4–9 (6 ± 1) x 7–13 (11 ± 2) Oesophageal (length x width) 446,165 × 54,477 238–410 (355 ± 44)× 45–54 (51 ± 2) Nerve-ring* 116,501 41-84 (61 ± 10) Vulva post-equatorial* 2.554,997 1.870–2.348 (1.988 ± 478) Tail length 158,277. 139-191 (160 ± 14) Eggs (length x width) 71,120 (77,845 – 65,744 ± 6,162) × 81 ± 4 × 43 ± 0.3 39,486 (43,638 – 34,621 ± 4,550) * Distance from anterior end.

Rhabdias occurrences in arboreal anurans are less (Araujo et al., 2015). The present first occurrence of common than in terrestrial species (Souza et Rhabdias cf. breviensis on S. acuminatus could be al., 2016; da Graça et al., 2017; Müller et al., 2018), associated to the fact that this parasite has been found this fact may be due treefrogs during the day remain in host sympatric species of S. acuminatus in Pantanal sheltered in the vegetation or in burrows, but at night wetland: Rhinella diptycha, Leptodactylus fuscus, and they exhibit terrestrial activity. The adults leave the L. podicipinus (Müller et al., 2018), which facilitate arboreal stratum to choose the breeding sites and deposit parasitic sharing as reported for other Rhabdias species their eggs in the water (Prado et al., 2005), during this (Junker et al., 2010; Langford and Janovy, 2013). course they can remain in the soil and come in contact with the infecting larvae (Pizzatto et al., 2014). Scinax Acknowledgement. The authors would like to thank the Embrapa Pantanal, who granted us access to Fazenda Nhumirim, and for acuminatus have a general diet (Duré, 2004) and during having provided support during the fieldwork. We also would like its foraging, this fact may allow the ingestion of infective to thank N. Mangini for reviewing the English. ICOS, MCM, and larvae of Rhabdias breviensis that are in the ground, or AMA received fellowships from Coordenação de Aperfeiçoamento present externally on the body of the prey (Langford de Pessoal de Nível Superior. PS received Fundação de Apoio ao and Janovy, 2009). Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Such factor may be linked to possible other host species Mato Grosso do Sul. GMM received fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico. The harbouring R. breviensis at the locality of study, due to Instituto Chico Mendes de Conservação da Biodiversidade/ the incorporation of this parasite in a new host being Sistema de Autorização e Informação em Biodiversidade (process related to infected species that are present in the same nº 57379-1) kindly provided a collecting permit. locality in which S. acuminatus was collected. Factor associated with the transmission of Rhabdias species References that occurs when infected hosts defecate infective larvae Aguiar, A., Toledo, G.M., Anjos, L.A., Silva, R.J. (2015): Helminth in the soil, so that they may come into contact with a parasite communities of two Physalaemus cuvieri Fitzinger, 1826 new host, either by active penetration into the skin or (Anura: Leiuperidae) populations under different conditions of by accidental ingestion of the larval forms present in integrity in the Atlantic Rain of Brazil. Brazilian the soil at the moment of foraging of the host (Baker, Journal of Biology 75: 963–968. 1979; Kuzmin et al., 2016). Therefore, the transmission Araujo, S.B.L., Braga, M.P., Brooks, D.R., Agosta, S.J., Hoberg, E.P., Hartentha, F.W., Boeger, W.A. (2015): Understanding host- is directly related to the existence of infected hosts in switching by ecological fitting. PLoS One 10: e0139225. the environment, which perpetuates colonization of the Baker, M.R. (1979): The free-living and parasitic development parasite in other species, a factor that is favoured also by of Rhabdias spp. (Nematoda: Rhabdiasidae) in amphibians. similar resources that the new host offers to the parasite Canadian Journal of Zoology 57: 161–178. First record of Rhabdias cf. breviensis parasitizing Scinax acuminatus, Brazil 979

Baker, M.R. (1987): Synopsis of the nematoda parasitic in Rhabdiaisidae): lungworms from snakes and anurans. Journal of amphibians and reptiles. Memorial University of Newfoundland. Parasitology 95: 1145–1155. Occasional Papers in Biology 11: 1–325. Langford, G.J., Janovy, J. (2013): Host Specificity of North Barrella, T.H, dos Santos, da Silva, R.J. (2010): Rhabdias American Rhabdias spp. (Nematoda: Rhabdiasidae): combining filicaudalis n. sp. (Nematoda: Rhabdiasidae) from the snake field data and experimental infections with a molecular Spilotes pullatus (Serpentes: Colubridae) in Brazil. Journal of phylogeny. Journal of Parasitology 99: 277–286. Helminthology 84: 292–296. Leivas, P.T., Leivas, F.W.T., Campião, K. (2018): Diet and parasites CONCEA - Conselho Nacional de Controle de Experimentação of the anuran Physalaemus cuvieri Fitzinger, 1826 (Leiuperidae) Animal (2013): Diretrizes da Prática de Eutanásia do from an Atlantic Forest fragment. Herpetology Notes 11: 109– CONCEA. 113. da Graça, R.J., Oda, F.H., Lima, F.S., Guerra V., Gambale, P.G., Martínez-Salazar, E.A., Pérez-Ponce de León, G.P.P., Olea, Takemoto, R.M. (2017): Metazoan endoparasites of 18 anuran G.P. (2009): First record of the genus Rhabdias (Nematoda: species from the mesophytic semideciduous Atlantic Forest in Rhabdiasidae), endoparasite from Scinax staufferi (Anura: southern Brazil. Journal of Natural History 51: 705–729. Hylidae) in . Revista Mexicana de Biodiversidad 80: Draghi, R., Lunaschi, L.I., Drago, F.B. (2015): First report of 861–865. helminth parasitizing Trachycephalus typhonius (Anura: McAllister, C.T., Bursey, C.R., Freed, P.S. (2010): Helminth Hylidae) from northeastern Argentina. Revista Mexicana de parasites of herpetofauna from the Rupunini District, Biodiversidad 86: 255–261. Southwestern Guyana. Comparative Parasitology 77: 184–201. Duré, M.I. (2004): Estructura trófica y aspectos ecológicos de Moretti, E.H., Titon Jr, B., Madelaire, C.B., Arruda, R., Alvareza, los gremios de una comunidad de anfibios de la provincia de T., Gomes, F.R. (2017): Behavioral, physiological and Corrientes. Unpublished PhD thesis, Universidad Nacional de morphological correlates of parasite intensity in the wild Cururu La Plata, La Plata, Argentina. (Rhinella icterica). International Journal for Parasitology: Frost, D.R. (2019): Amphibian Species of the World. American Parasites and Wildlife 6: 146–154. Museum of Natural History. Available at: http://research.amnh. Müller, M.I, Morais, D.H., Costa-Silva, G.J., Aguiar, A., Ávila, org/herpetology/amphibia/index.html. Accessed on 17 March R.W., Silva, R.J. (2018): Diversity in the genus Rhabdias 2019. (Nematoda, Rhabdiasidae): evidence for cryptic speciation. González, C.E., Hamann, M.I. (2007): Natural history notes. Zoologica Scripta 47: 595–607. Chaunus bergi (NCN). Endoparasites. Herpetological Review Nascimento, L.C.S., Gonçalves, E.C., Melo, F.T., Giese, E.G., 38: 181. Furtado, A.P., Santos, J.N. (2013): Description of Rhabdias González, C.E., Hamann, M.I. (2008): Nematode parasites of two breviensis n. sp. (Rhabditoidea: Rhabdiasidae) in two Neotropical anuran species Rhinella schneideri (Bufonidae) and Scinax species. Systematic Parasitology 86: 69 –75. acuminatus (Hylidae) from Corrientes, Argentina. Revista de Oliveira, C.R., Ávila, R.W., Morais, D.H. (2019): Helminths Biología Tropical 56: 2147–2161. associated with three Physalaemus species (Anura: González, C.E., Hamann, M.I. (2010): Larval nematodes found in Leptodactylidae) from Caatinga Biome, Brazil. Acta amphibians from northeastern Argentina. Brazilian Journal of Parasitologica 4: 205–212. Biology 70: 1089–1092. Pizzatto, L., Both, C., Shine, R. (2014): Quantifying anuran González, C.E., Hamann, M.I. (2016): Nematode parasites of microhabitat use to infer the potential for parasite transmission Leptodactylus elenae and Leptodactylus podicipinus (Anura: between invasive cane and two species of Australian Leptodactylidae) from Corrientes, Argentina. Comparative native . PloS One 9: e106996. Parasitology 83: 117–121. Prado, C.P.A., Haddad, C.F.B. (2005): Size-fecundity relationships Hamann, M.L., Kehr, A.L., González, C.E. (2013): Helminth and reproductive investment in female frogs in the Pantanal, communities in the burrowing toad, , from south-western Brazil. Herpetological Journal 15: 181–189. Northeastern Argentina. Biologia 68: 1155–1162. Prado, C.P.A., Uetanabaro, M., Haddad, C.F.B. (2005): Breeding Hamann, M.L., González, C.E. (2017): Helminth parasites in the activity patterns, reproductive modes, and habitat use by anurans toad Rhinella major (Bufonidae) from Chaco region, Argentina. (Amphibian) in a seasonal environment in the Pantanal, Brazil. Acta Herpetologica 10: 93–101. Amphibia-Reptilia 26: 211–221. Junker, K., Lhermitte-Vallarino, N., Barbuto, M., Ineich, I., Wanji, Santos, J.N., Melo, F.T.V., Nascimento, L.C.S., Nascimento, S., Bain, O. (2010): New species of Rhabdias (Nematoda: D.E.B., Giese, E.G., Furtado, A.P. (2011): Rhabdias paraensis Rhabdiasidae) from Afrotropical anurans, including molecular sp. nov.: a parasite of the lungs of Rhinella marina (Amphibia: evidence and notes on biology. Folia Parasitologica 57: 47–61. Bufonidae) from Brazilian Amazônia. Memórias do Instituto Kloss, G.R. (1971): Alguns Rhabdias (Nematoda) de Bufo no Oswaldo Cruz 106: 433–440. Brasil. Papéis Avulsos de Zoologia 24: 1–52. Sena, P.A., Conceição, B.M., Silva, P.F., Silva, W.G.O., Ferreira, Kuzmin, Y., Melo, F.T.V., Silva Filho, H.F., Santos, J.N. (2016): W.B., Silva Júnior, V.A., Moura, G.J.B., Oliveira, J.B. (2018): Two new species of Rhabdias Stiles et Hassall, 1905 (Nematoda: Helminth communities of Pithecopus nordestinus (Anura: Rhabdiasidae) from anuran amphibians in Pará, Brazil. Folia Phyllomedusidae) in forest remnants, Brazil. Herpetology Notes Parasitologica 63: 15. 11: 565–572. Langford, G.J., Janovy, J. (2009): Comparative life cycles and Silva, C.S., Ávila, R.W., Morais, D.H. (2018): Helminth community life histories of North American Rhabdias spp. (Nematoda: dynamics in a population of Pseudopaludicola pocoto 980 Isabela Caroline Oliveira da Silva et al.

(Leptodactylidae: Leiuperinae) from Northeast-Brazilian. Helminthologia 55: 292–305. Silva, I.C.O., Pacheco, E.O., Silva, L.A., Carvalho, P.S., Santana, D.J., Tavares, L.E.R. (2018): Metazoan parasites of Odontophrynus americanus (Anura: Odontophrynidae) from the Serra da Bodoquena mountain range, Mato Grosso do Sul, Brazil. Herpetology Notes 11: 911–913. Smales, L.R. (2007): Acanthocephala in amphibians (Anura) and reptiles (Squamata) from Brazil and Paraguay with description of a new species. Journal of Parasitology 93: 392–398. Souza, F.C., Forster, O.C., Queiroz, M.S., Anjos, L.A. (2016): First report of Rhabdias (Nematoda: Rhabdiasidae) in Hypsiboas raniceps, Cerrado, Brazil. Neotropical Helminthology 10: 295–300. Sueldo, C., Ramirez, V.G. (1976): Aportes sobre parásitos de Bufo arenarum de la provincia de Salta (Nematoda). Neotropica 22: 105–106. Teles, D.A., Brito, S.V., Filho, J.A.A., Ribeiro, S.C., Teixeira, A.A.M., Mesquita, D.O., Almeida, W.O. (2018a): Nematodes of the Rhinella granulosa Spix, 1824 (Anura: Bufonidae) from the Semiarid Northeastern Caatinga region of Brazil. Comparative Parasitology 85: 208–211. Teles, D. A., Pinto, C.L.M., Teixeira, A.A.M., Filho, J.A.A. (2018b): First report of Rhabdias sp. infecting Leptodactylus macrosternum from the Caatinga domain, Neotropical region. Cuadernos de herpetología 32: 117–118. Toledo, G.M., Fonseca, M.G., Iannacone, J., Callirgos, J.M.C., Vidaurre, C.U.M., Silva, R.J. (2017): Helminth parasites of Rhinella marina (Linnaeus, 1758) (Anura: Bufonidae) from Tarapoto, Peru. The Biologist (Lima) 15: 459–468. Vicente, J.J., Rodrigues, H.O., Gomes, D.C., Pinto, R.M. (1991): Nematoides do Brasil. 2a parte: Nematóides de Anfíbios. Revista Brasileira de Zoologia 7: 549–626.

Accepted by Fabrício Oda