The Man-Eating Lions of Tsavo
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RESEARCH ARTICLE Anti-Glioma Effect of Pseudosynanceia
DOI:10.31557/APJCP.2021.22.7.2295 Effect of Pseudosynanceia Melanostigma Venom on Glioblastoma Cells RESEARCH ARTICLE Editorial Process: Submission:02/14/2021 Acceptance:07/15/2021 Anti-Glioma Effect of Pseudosynanceia Melanostigma Venom on Isolated Mitochondria from Glioblastoma Cells Maral Ramezani, Fatemeh Samiei, Jalal Pourahmad* Abstract Background: Glioblastoma is the most common primary malignant tumor of the central nervous system that occurs in the spinal cord or brain. Pseudosynanceia Melanostigma is a venomous stonefish in the Persian Gulf, which our knowledge about is little. This study’s goal is to investigate the toxicity of stonefish crude venom on mitochondria isolated from U87 cells. Methods: In the first stage, we extracted venom stonefish and then isolated mitochondria have exposed to different concentrations of venom. Finally, mitochondrial toxicity parameters (Succinate dehydrogenase (SDH) activity, Reactive oxygen species (ROS), cytochrome c release, Mitochondrial Membrane Potential (MMP), and mitochondrial swelling) have evaluated. Results: To determine mitochondrial parameters, we used 115, 230, and 460 µg/ml concentrations. The results of our study show that the venom of stonefish selectively increases upstream parameters of apoptosis such as mitochondrial swelling, cytochrome c release, MMP collapse and ROS. Conclusion: This study suggests that Pseudosynanceia Melanostigma crude venom has selectively caused toxicity by increasing active mitochondrial oxygen radicals. This venom could potentially be a candidate for the treatment of glioblastoma. Keywords: Mitochondria- glioblastoma- fish venoms- cell line- tumor- toxicity-Persian Gulf Asian Pac J Cancer Prev, 22 (7), 2295-2302 Introduction freshwater or saltwater. Marine animals most commonly used for animals live in saltwater, i.e. in oceans, seas, Glioblastoma multiform is the most common group of etc. -
Spider Bites
Infectious Disease Epidemiology Section Office of Public Health, Louisiana Dept of Health & Hospitals 800-256-2748 (24 hr number) www.infectiousdisease.dhh.louisiana.gov SPIDER BITES Revised 6/13/2007 Epidemiology There are over 3,000 species of spiders native to the United States. Due to fragility or inadequate length of fangs, only a limited number of species are capable of inflicting noticeable wounds on human beings, although several small species of spiders are able to bite humans, but with little or no demonstrable effect. The final determination of etiology of 80% of suspected spider bites in the U.S. is, in fact, an alternate diagnosis. Therefore the perceived risk of spider bites far exceeds actual risk. Tick bites, chemical burns, lesions from poison ivy or oak, cutaneous anthrax, diabetic ulcer, erythema migrans from Lyme disease, erythema from Rocky Mountain Spotted Fever, sporotrichosis, Staphylococcus infections, Stephens Johnson syndrome, syphilitic chancre, thromboembolic effects of Leishmaniasis, toxic epidermal necrolyis, shingles, early chicken pox lesions, bites from other arthropods and idiopathic dermal necrosis have all been misdiagnosed as spider bites. Almost all bites from spiders are inflicted by the spider in self defense, when a human inadvertently upsets or invades the spider’s space. Of spiders in the United States capable of biting, only a few are considered dangerous to human beings. Bites from the following species of spiders can result in serious sequelae: Louisiana Office of Public Health – Infectious Disease Epidemiology Section Page 1 of 14 The Brown Recluse: Loxosceles reclusa Photo Courtesy of the Texas Department of State Health Services The most common species associated with medically important spider bites: • Physical characteristics o Length: Approximately 1 inch o Appearance: A violin shaped mark can be visualized on the dorsum (top). -
Violent Incidents Between Humans and Orcas in Captivity
Violent incidents between humans and orcas in captivity Several accounts of violent incidents with humans have appeared in books and news clips, with little information on the dates or details of those incidents. Other descriptions have made headlines, and some were captured on video tape. There are also anecdotal reports of incidents that were never officially documented. NO. DATE AQUARIUM WHALEs INCIDENT SOURCE early years New York When water level was lowered for pool cleaning, young female Lupa sent Edward R. Riciuti, , New #1 1968 Lupa York, Walker & Co., 1973, Aquarium, USA trainers scrambling from the pool, snapping her jaws threatening. pp. 227-228. Edward R. Riciuti, Killers of the Sea, New York, Young male Cuddles became so increasingly aggressive, having a hold of at Walker & Co., 1973, pp. Flamingo Park, least two trainers, that keepers had to clean the pool from the protection of a 227-228; Reading #2 1969-1970 Cuddles England shark cage. Cuddles also dragged keeper Don Robinson into the pool when he Eagle, August 15, was at Dudley Zoo but that was possibly a PR stunt. 1971; Doug Cartlidge, personal communication, March 2010. Karen Pryor writes, "I have since heard... of at least one killer whale which Karen Pryor, Lads Before the Wind, New York, #3 1970s unknown unknown launched an unprovoked attack on a favorite trainer, in normal circumstances, Harper & Row, 1976, p. savaged him very badly, and nearly killed him." 220. Vancouver Trainer Doug Pemberton described young female Skana as the dominant Cranky killer whales put trainers through their #4 1970's Aquarium, Skana animal in the pool. -
The Status of Kenya's Elephants
The status of Kenya’s elephants 1990–2002 C. Thouless, J. King, P. Omondi, P. Kahumbu, I. Douglas-Hamilton The status of Kenya’s elephants 1990–2002 © 2008 Save the Elephants Save the Elephants PO Box 54667 – 00200 Nairobi, Kenya first published 2008 edited by Helen van Houten and Dali Mwagore maps by Clair Geddes Mathews and Philip Miyare layout by Support to Development Communication CONTENTS Acknowledgements iv Abbreviations iv Executive summary v Map of Kenya viii 1. Introduction 1 2. Survey techniques 4 3. Data collection for this report 7 4. Tsavo 10 5. Amboseli 17 6. Mara 22 7. Laikipia–Samburu 28 8. Meru 36 9. Mwea 41 10. Mt Kenya (including Imenti Forest) 42 11. Aberdares 47 12. Mau 51 13. Mt Elgon 52 14. Marsabit 54 15. Nasolot–South Turkana–Rimoi–Kamnarok 58 16. Shimba Hills 62 17. Kilifi District (including Arabuko-Sokoke) 67 18. Northern (Wajir, Moyale, Mandera) 70 19. Eastern (Lamu, Garissa, Tana River) 72 20. North-western (around Lokichokio) 74 Bibliography 75 Annexes 83 The status of Kenya’s elephants 1990–2002 AcKnowledgemenTs This report is the product of collaboration between Save the Elephants and Kenya Wildlife Service. We are grateful to the directors of KWS in 2002, Nehemiah Rotich and Joseph Kioko, and the deputy director of security at that time, Abdul Bashir, for their support. Many people have contributed to this report and we are extremely grateful to them for their input. In particular we would like to thank KWS field personnel, too numerous to mention by name, who facilitated our access to field records and provided vital information and insight into the status of elephants in their respective areas. -
Awareness, Prevention and Treatment of World-Wide Marine Stings and Bites
Awareness, Prevention and Treatment of world-wide marine stings and bites Dr Peter Fenner Honorary Medical Officer, Surf Life Saving Australia International Life Saving Federation Medical/Rescue Conference Proceedings September 1997 Abstract The most common world-wide first aid treatment used by the average lifesaver/lifeguard is the treatment of marine envenomation, especially the treatment of jellyfish stings. It is important to use the correct first aid treatment for each type of envenomation. This study provides a simplified protocol for: - 1. Awareness of the geographical distribution and possibilities of envenomation enabling: - 2. Preventative strategies to reduce morbidity and mortality from marine envenomation 3. First aid treatment of marine envenomation by jellyfish or other marine animals This discussion is based on protocols developed for Surf Life Saving Australia and other first aid providers in Australia over the past ten years. Their success has been proven by a 30% reduction in the number of stings over the past 10 years (statistics from the author’s records). Information for this article has been taken from: - 1. Venomous and poisonous marine animals: a medical and biological handbook produced by Surf Life Saving Queensland 2. The global problem of cnidarian stinging. MD Thesis by the author for the University of London. Introduction The global problem of marine envenomation is not fully appreciated. Each year hundreds of deaths occur from poisoning (by ingestion or eating) or by envenomation (stinging by jellyfish, or biting by venomous marine animals). The morbidity is even greater with jellyfish stings world-wide being numbered in their millions. Each summer it is estimated that up to half a million stings occur on the east coast of the United States from the Portuguese man-o’-war (Physalia physalis). -
A Guide to Harmful and Toxic Creatures in the Goa of Jordan
Published by the Royal Marine Conservation Society of Jordan. P. O. Box 831051, Abdel Aziz El Thaalbi St., Shmesani 11183. Amman Copyright: © The Royal Marine Conservation Society of Jordan Reproduction of this publication for educational and other non- commercial purposes is authorized without prior written approval from the copyright holder provided the source is fully acknowledged. ISBN: 978-9957-8740-1-8 Deposit Number at the National Library: 2619/6/2016 Citation: Eid, E and Al Tawaha, M. (2016). A Guide to Harmful and Toxic Creature in the Gulf of Aqaba of Jordan. The Royal Marine Conservation Society of Jordan. ISBN: 978-9957-8740-1-8. Pp 84. Material was reviewed by Dr Nidal Al Oran, International Research Center for Water, Environment and Energy\ Al Balqa’ Applied University,and Dr. Omar Attum from Indiana University Southeast at the United State of America. Cover page: Vlad61; Shutterstock Library All photographs used in this publication remain the property of the original copyright holder, and it should not be reproduced or used in other contexts without permission. 1 Content Index of Creatures Described in this Guide ......................................................... 5 Preface ................................................................................................................ 6 Part One: Introduction ......................................................................................... 8 1.1 The Gulf of Aqaba; Jordan ......................................................................... 8 1.2 Aqaba; -
Dietary Behaviour of Man-Eating Lions As Revealed by Dental Microwear Textures Received: 29 November 2016 Larisa R
www.nature.com/scientificreports OPEN Dietary behaviour of man-eating lions as revealed by dental microwear textures Received: 29 November 2016 Larisa R. G. DeSantis1 & Bruce D. Patterson2 Accepted: 20 March 2017 Lions (Panthera leo) feed on diverse prey species, a range that is broadened by their cooperative Published: xx xx xxxx hunting. Although humans are not typical prey, habitual man-eating by lions is well documented. Fathoming the motivations of the Tsavo and Mfuwe man-eaters (killed in 1898 in Kenya and 1991 in Zambia, respectively) may be elusive, but we can clarify aspects of their behaviour using dental microwear texture analysis. Specifically, we analysed the surface textures of lion teeth to assess whether these notorious man-eating lions scavenged carcasses during their depredations. Compared to wild-caught lions elsewhere in Africa and other large feliforms, including cheetahs and hyenas, dental microwear textures of the man-eaters do not suggest extreme durophagy (e.g. bone processing) shortly before death. Dental injuries to two of the three man-eaters examined may have induced shifts in feeding onto softer foods. Further, prompt carcass reclamation by humans likely limited the man-eaters’ access to bones. Man-eating was likely a viable alternative to hunting and/or scavenging ungulates due to dental disease and/or limited prey availability. Lions (Panthera leo) once inhabited much of Africa, southeastern Europe, and southwestern Asia1. Currently, lions (Panthera leo) occupy savannas and deserts in sub-Saharan Africa (excluding rainforests and the Sahara), with an isolated population located in the Gir Forest of India. They are highly social, and males and females each live in persistent bonded groups2. -
Dangerous Creatures of the Maltese Sea: Injuries and Treatment - Part 2
REVIEW ARTICLE Dangerous creatures of the Maltese sea: Injuries and treatment - Part 2 DR DAVID JAMES SAMMUT ABSTRACT deeper than the epidermis. The common Mediterranean This is the second of a two-part article intended sea urchin does not possess any venom in its spines, thus to give information about different organisms which injury is often of a minor nature. inhabit the Maltese sea and which are potentially harmful. Doctors working in the primary health setup Treatment and sometimes also in secondary care are often faced Treatment options vary. Embedded spines can either with injuries related to these organisms. The nature of be removed immediately, after a few days, or left to come the injury and its treatment is then discussed. Treatment out spontaneously. Any large projecting spines should however is not evidenced based as little if any studies be carefully removed with tweezers (Drobina, 2008). have been conducted in this field of medicine. After the Deeper spines are more difficult to remove. Removing first article considered venomous organisms, this second them immediately is often time consuming, painful and article will review sea creatures that may cause injury may cause more trauma to the site. Superficial spines are through bites, spines and electricity. best left undisturbed, as they will eventually be rejected spontaneously as the epidermis renews itself. Deeper KEY WORDS spines will come out easier if left for a few days before Bites, moray eel bites, sea urchin puncture attempting to remove them. The skin over the spine is broken with a needle and the spine is easily removed by INTRODUCTION applying pressure at the sides of the spine. -
A Summary of the Effects of Captivity on Orcas
A Summary of the Effects of Captivity on Orcas PEOPLE FOR THE ETHICAL TREATMENT OF ANIMALS Contents The Eff ects of Captivity on Tilikum and Orcas Generally at SeaWorld…………..................................…………......3 I. Orcas Are Extremely Intelligent Mammals Whose Brains Are Highly Developed in Areas Responsible for Complex Cognitive Functions, Including Self-Awareness, Social Cognition, Culture, and Language …………………………………………...............................................................................................…...4 II. Tilikum Is Deprived of Every Facet of His Culture and the Opportunity to Engage in Natural Behavior, Causing Extreme Stress and Suff ering….…………….….......................................................5 A. The Tanks at SeaWorld Provide Inadequate Space and Result in Stress……….…...........................5 B. SeaWorld’s Constant Manipulation of Tilikum’s Social Structure Results in Stress.................7 C. The Tanks at SeaWorld Create a Distressing Acoustic Environment…….………..….........................9 III. The Stressors of the Captive Environment at SeaWorld Result in Aggressiveness, Self- Injury, and Other Physical and Behavioral Abnormalities………………….……..............................................10 A. Aggression Between Orcas and Between Orcas and Humans……..……………..............................……10 B. Stereotypic Behavior………………….……………………………………….......................................................................….…..13 1. Painful Dental Problems Caused by Chewing Metal Gates and Concrete Tanks.....14 2. -
Investigative Guidelines: Animal Bites and Rabies
PUBLIC HEALTH DIVISION Acute and Communicable Disease Prevention Animal Bites and Rabies Investigative Guidelines December 2018 1. DISEASE REPORTING 1.1 Purpose of Reporting and Surveillance 1. To assess the risk of rabies in persons bitten or otherwise possibly exposed to recommend rabies post-exposure prophylaxis (RPEP) to those who need it, and to provide counseling and reassurance to those who don’t. 2. As necessary to arrange for the capture and either confinement (10-day observation) of a live dog, cat or ferret, or the laboratory examination of an animal head. This may involve coordination with other agencies, e.g., the Humane Society, county sanitarians, animal control and local law enforcement. 3. To identify zoonotic sources of infection. 1.2 Reporting Requirements 1. Anyone with knowledge of humans being bitten by potentially rabid animals (e.g., physicians, veterinarians, animal control personnel, law-enforcement officials, or animal owners), is required to report such incidents to the Local Health Department (LHD) within one working day. 2. Laboratories: Any confirmed case of rabies in an animal and any suspected or confirmed case of human rabies must be reported immediately (day or night) to the LHD. If the LHD cannot be reached, the Acute and Communicable Disease Prevention (ACDP) Section of Oregon Health Authority (OHA) should be contacted at 971-673-1111. 1.3 Local Health Department Reporting and Follow-Up Responsibilities 1. Investigate all reports of animal bites, on the day of the report whenever possible. 2. Determine, in consultation with OHA on-call staff as necessary, whether the exposure constitutes a significant risk for rabies, in which either empiric RPEP or testing of the animal is to be recommended. -
Petition to Reclassify and Uplist African Elephants
BEFORE THE SECRETARY OF THE INTERIOR Photo by Tara Easter PETITION TO RECLASSIFY AND UPLIST AFRICAN ELEPHANTS FROM THREATENED TO ENDANGERED UNDER THE ENDANGERED SPECIES ACT AS TWO SEPARATE SPECIES: FOREST ELEPHANTS (Loxodonta cyclotis) AND SAVANNAH ELEPHANTS (Loxodonta africana) JUNE 10, 2015 CENTER FOR BIOLOGICAL DIVERSITY NOTICE OF PETITION Sally Jewell, Secretary U.S. Department of the Interior 1849 C Street NW Washington, D.C. 20240 [email protected] Dan Ashe, Director U.S. Fish and Wildlife Service 1849 C Street NW Washington, D.C. 20240 [email protected] Douglas Krofta, Chief Branch of Listing, Endangered Species Program U.S. Fish and Wildlife Service 4401 North Fairfax Drive, Room 420 Arlington, VA 22203 [email protected] PETITIONER The Center for Biological Diversity (Center) is a non-profit, public interest environmental organization dedicated to the protection of native species and their habitats through science, policy, and environmental law. The Center is supported by more than 900,000 members and activists throughout the United States. The Center and its members are concerned with the conservation of endangered species and the effective implementation of the Endangered Species Act. Submitted this 10th day of June, 2015 Pursuant to Section 4(b) of the Endangered Species Act (ESA), 16 U.S.C. § 1533(b); section 553(e) of the Administrative Procedure Act (APA), 5 U.S.C. § 553(e); and 50 C.F.R. § 424.14(a), the Center for Biological Diversity hereby petitions the Secretary of the Interior, through the U.S. Fish and Wildlife Service (FWS or Service), to reclassify and uplist African elephants from Threatened to Endangered as two separate species: forest elephants, (Loxodonta cyclotis) and savannah elephants (Loxodonta africana). -
PRICK TESTING in INSECT BITE REACTION Dissertation Submitted
PRICK TESTING IN INSECT BITE REACTION Dissertation submitted to The Tamil Nadu Dr. M.G.R Medical University, Chennai In fulfilment of the requirements for the award of the degree of Doctor of Medicine in Dermatology, Venereology and Leprology Under the guidance of Dr. SHANMUGA SEKAR .C, MD., Department of Dermatology, Venereology and Leprology PSG INSTITUTE OF MEDICAL SCIENCES & RESEARCH, COIMBATORE THE TAMILNADU DR. M.G.R MEDICAL UNIVERSITY, CHENNAI, TAMILNADU MAY 2018 CERTIFICATE This is to certify that the thesis entitled “PRICK TESTING IN INSECT BITE REACTION” is a bonafide work of Dr. IYSHWARIYA SIVADASAN done under the direct guidance and supervision of Dr.C.R. SRINIVAS, MD and Dr. SHANMUGA SEKAR .C, MD, in the department of Dermatology, Venereology and Leprology, PSG Institute of Medical Sciences and Research, Coimbatore in fulfillment of the regulations of The Tamil Nadu Dr.MGR Medical University for the award of MD degree in Dermatology, Venereology and Leprology. Dr. REENA RAI Dr. RAMALINGAM Professor & Head of Department DEAN Department of DVL DECLARATION I hereby declare that this dissertation entitled “PRICK TESTING IN INSECT BITE REACTION” was prepared by me under the direct guidance and supervision of Dr.C.R.SRINIVAS, MD and Dr. SHANMUGA SEKAR C., MD, PSG Institute of Medical Sciences and Research, Coimbatore. The dissertation is submitted to The Tamil Nadu Dr. MGR Medical University in fulfillment of the University regulation for the award of MD degree in Dermatology, Venereology and Leprology. This dissertation has not been submitted for the award of any other Degree or Diploma. Dr. IYSHWARIYA SIVADASAN CERTIFICATE BY THE GUIDE This is to certify that the thesis entitled “PRICK TESTING IN INSECT BITE REACTION” is a bonafide work of Dr.