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Contents • March 2020 • Volume 94, No. 5 CONTENTS • MARCH 2020 • VOLUME 94, NO. 5 COVER IMAGE Cover photograph: All members of the Herpesviridae encode the conserved herpesvirus protein kinase (CHPK). Mutation of the invariant lysine required for kinase activity of Marek’s disease alphaherpesvirus (MDV) CHPK abrogates interindividual spread in chickens. MDV spreads from chicken-to-chicken in a fashion similar to that of varicella-zoster virus in humans in that it is shed from the skin of infected hosts and enters the naive host through the respiratory route. We found that without CHPK activity, late gene expression is severely affected in the skin of infected chickens, suggesting that CHPK is required for generation of infectious virus in the skin. (See related article at e01522-19.) (Copyright © 2020 American Society for Microbiology. All Rights Reserved.) SPOTLIGHT Rozanne M. R Articles of Significant Interest in This Issue e02146-19 Sandri-Goldin Spotlight STRUCTURE AND ASSEMBLY Tom Gallagher T Molecular Basis of Binding between Middle East Respiratory Syndrome e01387-19 Structure Coronavirus and CD26 from Seven Bat Species Yuan Yuan, Jianxun Qi, Ruchao Peng, Chunrui Li, Guangwen Lu, Jinghua Yan, Qihui Wang, George Fu Gao Frank Kirchhoff T Biochemical Reconstitution of HIV-1 Assembly and Maturation e01844-19 Structure Iga Kucharska, Pengfei Ding, Kaneil K. Zadrozny, Robert A. Dick, Michael F. Summers, Barbie K. Ganser-Pornillos, Owen Pornillos Rozanne M. T Glycoprotein C of Herpes Simplex Virus 1 Shields Glycoprotein B from Antibody e01852-19 Sandri-Goldin Structure Neutralization Tri Komala Sari, Katrina A. Gianopulos, Anthony V. Nicola GENOME REPLICATION AND REGULATION OF VIRAL GENE EXPRESSION Julie K. Pfeiffer T Different Degrees of 5’-to-3’ DAR Interactions Modulate Zika Virus Genome e01602-19 Replication Cyclization and Host-Specific Replication Xiao-Dan Li, Cheng-Lin Deng, Zhi-Ming Yuan, Han-Qing Ye, Bo Zhang Lawrence Banks T The Ubiquitin-Specific Protease Usp7, a Novel Merkel Cell Polyomavirus Large e01638-19 Replication T-Antigen Interaction Partner, Modulates Viral DNA Replication Manja Czech-Sioli, Svenja Siebels, Sonja Radau, René P. Zahedi, Claudia Schmidt, Thomas Dobner, Adam Grundhoff, Nicole Fischer Rozanne M. T RNA Polymerase II Promoter-Proximal Pausing and Release to Elongation Are e02035-19 Sandri-Goldin Replication Key Steps Regulating Herpes Simplex Virus 1 Transcription Claire H. Birkenheuer, Joel D. Baines GENETIC DIVERSITY AND EVOLUTION Colin R. Parrish T Evolutionary Dynamics of Oropouche Virus in South America e01127-19 Diversity & Evol Bernardo Gutierrez, Emma L. Wise, Steven T. Pullan, Christopher H. Logue, Thomas A. Bowden, Marina Escalera-Zamudio, Gabriel Trueba, Marcio R. T. Nunes, Nuno R. Faria, Oliver G. Pybus Guido Silvestri T Impact of Suboptimal APOBEC3G Neutralization on the Emergence of HIV Drug e01543-19 Diversity & Evol Resistance in Humanized Mice Matthew M. Hernandez, Audrey Fahrny, Anitha Jayaprakash, Gustavo Gers-Huber, Marsha Dillon-White, Annette Audigé, Lubbertus C. F. Mulder, Ravi Sachidanandam, Roberto F. Speck, Viviana Simon Instructions to Authors are available on the journal website. CONTENTS • MARCH 2020 • VOLUME 94, NO. 5 Colin R. Parrish T Influenza B Viruses Exhibit Lower Within-Host Diversity than Influenza A Viruses e01710-19 Diversity & Evol in Human Hosts Andrew L. Valesano, William J. Fitzsimmons, John T. McCrone, Joshua G. Petrie, Arnold S. Monto, Emily T. Martin, Adam S. Lauring Guido Silvestri T Genetic Diversity, Compartmentalization, and Age of HIV Proviruses Persisting in e01786-19 Diversity & Evol CD4؉ T Cell Subsets during Long-Term Combination Antiretroviral Therapy Bradley R. Jones, Rachel L. Miller, Natalie N. Kinloch, Olivia Tsai, Hawley Rigsby, Hanwei Sudderuddin, Aniqa Shahid, Bruce Ganase, Chanson J. Brumme, Marianne Harris, Art F. Y. Poon, Mark A. Brockman, Rémi Fromentin, Nicolas Chomont, Jeffrey B. Joy, Zabrina L. Brumme VIRUS-CELL INTERACTIONS Lawrence Banks T JC Polyomavirus Infection Reveals Delayed Progression of the Infectious Cycle in e01331-19 Interactions Normal Human Astrocytes Michael P. Wilczek, Jeanne K. DuShane, Francesca J. Armstrong, Melissa S. Maginnis Richard M. T Kaposi’s Sarcoma-Associated Herpesvirus Viral Interleukin-6 Signaling e01384-19 Longnecker Interactions Upregulates Integrin ␤3 Levels and Is Dependent on STAT3 Ricardo Rivera-Soto, Nathan J. Dissinger, Blossom Damania Richard M. T Endoplasmic Reticulum (ER) Reorganization and Intracellular Retention of CD58 e01435-19 Longnecker Interactions Are Functionally Independent Properties of the Human Cytomegalovirus ER-Resident Glycoprotein UL148 Christopher C. Nguyen, Anthony J. Domma, Hongbo Zhang, Jeremy P. Kamil J.-H. James Ou T PACSIN2 Interacts with Nonstructural Protein 5A and Regulates Hepatitis C Virus e01531-19 Interactions Assembly Lap P. Nguyen, Si C. Tran, Shiro Suetsugu, Yun-Sook Lim, Soon B. Hwang Jae U. Jung T Induction of Kaposi’s Sarcoma-Associated Herpesvirus-Encoded Thymidine Kinase e01555-19 Interactions (ORF21) by X-Box Binding Protein 1 Victoria Wang, David A. Davis, Claire Deleage, Catherine Brands, Hong S. Choi, Muzammel Haque, Robert Yarchoan Jae U. Jung T Kaposi’s Sarcoma-Associated Herpesvirus LANA Modulates the Stability of the E3 e01578-19 Interactions Ubiquitin Ligase RLIM Hagar Tadmor, Melanie Greenway, Anuj Ahuja, Ola Orgil, Gangling Liao, Richard F. Ambinder, S. Diane Hayward, Meir Shamay Tom Gallagher T Trypsin Treatment Unlocks Barrier for Zoonotic Bat Coronavirus Infection e01774-19 Interactions Vineet D. Menachery, Kenneth H. Dinnon III, Boyd L. Yount, Jr., Eileen T. McAnarney, Lisa E. Gralinski, Andrew Hale, Rachel L. Graham, Trevor Scobey, Simon J. Anthony, Lingshu Wang, Barney Graham, Scott H. Randell, W. Ian Lipkin, Ralph S. Baric Rozanne M. T ␤-Catenin Is Required for the cGAS/STING Signaling Pathway but Antagonized e01847-19 Sandri-Goldin Interactions by the Herpes Simplex Virus 1 US3 Protein Hongjuan You, Yingying Lin, Feng Lin, Mingyue Yang, Jiahui Li, Rongzhao Zhang, Zhiming Huang, Qingtang Shen, Renxian Tang, Chunfu Zheng Stacey T Influenza Virus Hemagglutinins H2, H5, H6, and H11 Are Not Targets of Pulmonary e01951-19 Schultz-Cherry Interactions Surfactant Protein D: N-Glycan Subtypes in Host-Pathogen Interactions Lisa M. Parsons, Yanming An, Li Qi, Mitchell R. White, Roosmarijn van der Woude, Kevan L. Hartshorn, Jeffery K. Taubenberger, Robert P. de Vries, John F. Cipollo iv jvi.asm.org Journal of Virology Volume 94 Issue 5 March 2020 CONTENTS • MARCH 2020 • VOLUME 94, NO. 5 Tom Gallagher T Molecular Mechanism for Antibody-Dependent Enhancement of Coronavirus e02015-19 Interactions Entry Yushun Wan, Jian Shang, Shihui Sun, Wanbo Tai, Jing Chen, Qibin Geng, Lei He, Yuehong Chen, Jianming Wu, Zhengli Shi, Yusen Zhou, Lanying Du, Fang Li Rozanne M. T Invasion of Herpes Simplex Virus 1 into Murine Dermis: Role of Nectin-1 and e02046-19 Sandri-Goldin Interactions Herpesvirus Entry Mediator as Cellular Receptors during Aging Lisa Wirtz, Maureen Möckel, Dagmar Knebel-Mörsdorf CELLULAR RESPONSE TO INFECTION Julie K. Pfeiffer T The IFNL4 Gene Is a Noncanonical Interferon Gene with a Unique but e01535-19 Response Evolutionarily Conserved Regulation Hao Zhou, Michelle Møhlenberg, Ewa Terczyn´ska-Dyla, Kasper Grønbjerg Winther, Nanna Hougaard Hansen, Johan Vad-Nielsen, Laura Laloli, Ronald Dijkman, Anders Lade Nielsen, Hans Henrik Gad, Rune Hartmann VACCINES AND ANTIVIRAL AGENTS Julie K. Pfeiffer T Astrovirus Replication Is Inhibited by Nitazoxanide In Vitro and In Vivo e01706-19 Vaccines Virginia Hargest, Bridgett Sharp, Brandi Livingston, Valerie Cortez, Stacey Schultz-Cherry Stacey T Monoclonal Antibodies with Neutralizing Activity and Fc-Effector Functions e01741-19 Schultz-Cherry Vaccines against the Machupo Virus Glycoprotein Fatima Amanat, James Duehr, Cheng Huang, Slobodan Paessler, Gene S. Tan, Florian Krammer Richard M. T Targeting Kaposi’s Sarcoma-Associated Herpesvirus ORF21 Tyrosine Kinase and e01791-19 Longnecker Vaccines Viral Lytic Reactivation by Tyrosine Kinase Inhibitors Approved for Clinical Use Guillaume Beauclair, Eleonora Naimo, Tatyana Dubich, Jessica Rückert, Sandra Koch, Akshay Dhingra, Dagmar Wirth, Thomas F. Schulz PATHOGENESIS AND IMMUNITY Richard M. T Herpes Simplex Virus 1-Specific CD8؉ T Cell Priming and Latent Ganglionic e01193-19 Longnecker Pathogenesis Retention Are Shaped by Viral Epitope Promoter Kinetics Benjamin R. Treat, Sarah M. Bidula, Anthony J. St. Leger, Robert L. Hendricks, Paul R. Kinchington Guido Silvestri T A MUC16 IgG Binding Activity Selects for a Restricted Subset of IgG Enriched for e01246-19 Pathogenesis Certain Simian Immunodeficiency Virus Epitope Specificities Jeffrey R. Schneider, Xiaoying Shen, Chiara Orlandi, Tinashe Nyanhete, Sheetal Sawant, Ann M. Carias, Archer D. Smith IV, Neil L. Kelleher, Ronald S. Veazey, George K. Lewis, Georgia D. Tomaras, Thomas J. Hope Stacey T Mutations in the Neuraminidase-Like Protein of Bat Influenza H18N11 Virus e01416-19 Schultz-Cherry Pathogenesis Enhance Virus Replication in Mammalian Cells, Mice, and Ferrets Gongxun Zhong, Shufang Fan, Masato Hatta, Sumiho Nakatsu, Kevin B. Walters, Tiago J. S. Lopes, Jessica I-Hsuan Wang, Makoto Ozawa, Alexander Karasin, Yan Li, Suxiang Tong, Ruben O. Donis, Gabriele Neumann, Yoshihiro Kawaoka Richard M. T Expression of the Conserved Herpesvirus Protein Kinase (CHPK) of Marek’s e01522-19 Longnecker Pathogenesis Disease Alphaherpesvirus in the Skin Reveals a Mechanistic Importance for CHPK during Interindividual Spread in Chickens Andrea Krieter, Nagendraprabhu
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