Health Sciences Is It Possible to Prepare Deuterium Depleted Water

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Health Sciences Is It Possible to Prepare Deuterium Depleted Water Clinical and Experimental Health Sciences Letter to Editor / Editöre Mektup Is it Possible to Prepare Deuterium Depleted Water at Home? Döteryumu Azaltılmış Suyu Evde Hazırlamak Mümkün mü? Burcin Alev Tuzuner1, Aysen Yarat1 1 Marmara University, Department of Basic Medical Sciences, Biochemistry, Istanbul, Turkey ORCID ID of the authors: B.A.T. 0000-0001-5122-4977, A.Y. 0000-0002-8258-6118 Cite this article as: Tuzuner Alev B., Yarat A. Is it possible to prepare deuterium depleted water at home? Clin Exp Health Sci 2018; 8: 226-227. DOI: 10.5152/ Citeclinexphealthsci.2018.1236 this article as: Tuzuner Alev B., Yarat A. Is it possible to prepare deuterium depleted water at home? Clin Exp Health Sci 2018. DOI: 10.5152/clinexphealthsci.2018.1236 Deuterium (D), also known as heavy hydrogen, is an isotope of hydrogen. Deuterium is essential for the normal growth of animal and plant cells. In natural water, deuterium/hydrogen (D/H) ratio is approximately 1/6600 (150 ppm). For deuterium depleted water (DDW or light water), this ratio is lower than 150 ppm, while for heavy water, it is higher than that (1). Previously, DDW has been used between 0.01 ppm and 145 ppm (2,3). Reportedly, DDW consumption is beneficial for diseases associated with high oxidative stress, such as cancer and diabetes, and has a protective effect against the complications of these diseases (4). DDW consumption may stimulate skin renewal; reduce inflammation; slow aging; and alleviate harmful effects of radiation (nausea, pain, and hair loss) (5,6). Regular consumption of DDW leads to the normalization of carbohydrate and lipid metabolisms, weight improvement, and the elimination of toxins from organs (7). The deuterium concentration of natural water varies with geographical regions. Natural light water is found in mountain rivers and melting glaciers and is rare and extremely limited throughout the world (Hunza region in Pakistan and the Himalayas) (1). Deuterium depletion naturally occurs in water. Since evaporation favors hydrogen over the heavier deuterium, water vapors contain lower deuterium concentrations (deuterium evaporates last and condenses first). In areas with a greater degree of evaporation (equator and deserts), the deuterium concentration of the surface water is high. Conversely, in areas with the smaller degree of evaporation (Polar Regions and mountains), the deuterium concentration of the surface water is low. Normal water freezes at 0°C, whereas heavy water freezes at 3.8°C (8). Water without heavy components (light water) can be prepared by different methods, including (a) fractional distillation (low separation coefficient, multiple-stage process, and expensive); (b) crystallization method (deuterium concentration can be reduced at most to 136 ppm); (c) the method involving platinum catalyst, which quickly removes deuterium from water using cold and hot temperatures (deuterium concentration can be reduced to 125 ppm); (d) separation with membrane (expensive, requires extra pure initial water, and deuterium concentration can be reduced to 117 ppm); (e) isotopic vacuum distillation technique (deuterium concentration can be reduced to 25 ppm); and (f) electrolytic process (water electrolysis using nickel electrodes, followed by the synthesis of water from protium and oxygen, and deuterium concentration can be reduced to 10 ppm) (9,10). DDW is available at different prices and in different brands globally. It is very expensive, and the cost rises further with the addition of import prices. Thus, methods to prepare DDW at home have been continuously investigated. There are many websites related to this subject. We aimed to provide a scientific answer to this question and attempted to obtain DDW from drinking water. We stored 200 mL of water sample from 19 L of drinking water carboy as the initial sample at 4°C until analysis. To prepare DDW, the remaining water was placed in small plastic containers and stored in a deep freezer at −18°C. After approximately 2–3 h, the frozen part was discarded, and the non-frozen part (water) was collected in a separate container. The same procedure was applied to the whole water. The collected non- frozen water was then subjected to the same treatment again. This process was repeated six times. Then, the initial and final water samples were sent to the isotope laboratory for analysis at the Technical Research and Quality Control Division of the State Hydraulic Works (DSI), Correspondence Author/Sorumlu Yazar: Burcin Alev Tuzuner E-mail/E-posta: [email protected] ©Copyright by 2018 Journal of Marmara University Institute of Health Sciences 226 Clin Exp Health Sci 2018; 8: 226-227 Tuzuner et al. Deuterium depleted water preparation Ankara. Deuterium isotope was measured according to the Vienna REFERENCES Standard Mean Ocean Water using internal standard calibration with [1] Somlyai G. Defeating Cancer. The Biological Effect of Deuterium the international standard by an isotopic water analyzer (Picarro Depletion. 2nd ed. Budapest: HYD Ltd. for Research and Development; L2130-i, Santa Clara, California, USA). 2002. Deuterium isotope content in water samples are expressed as δ [2] Somlyai G. Use of deuterium depleted water for the treatment of insulin values (δ²H), which are permil deviations from an internationally resistance. 2014; U.S. Patent Application No. 13/808,074. accepted standard. The formula to convert δ value to ppm unit is [3] Proceedings of 2nd International Congress on Deuterium Depletion; shown below . 2012 May 17-18; Budapest, Hungary. In: Eur Chem Bull 2012; 1(1): 39-47. [4] Yarat A. The biological importance of deuterium in drinking water (in While the deuterium concentration of drinking water at the beginning Turkish). Proceedings of DSI, 4th.National Hydrology Isotope Technics was 147 ppm, this value decreased to 144 ppm at the end. Symposium; 2015 Oct 05-09; Istanbul, Turkey. 2015. p:211-221. In conclusion, it is possible to prepare DDW using a large quantity of [5] Ávila DS, Somlyai G, Somlyai I, Aschner M. Anti-aging effects of deuterium depletion on Mn-induced toxicity in a C. Elegans Model. water initially and by repeating the above mentioned process for more Toxicol Lett 2012; 211: 319– 324. than six times. However, considering that we drink 1.5–2 L water daily, this method is not practical since much water would be required. DDW [6] Mosin O, Ignatov I. Studying of isotopic effects of deuterium in obtained through this method can be used in scientific studies. biological objects. Eur Rev Chem Res 2015; 3(1): 25-42. [7] Yarat A, Saçan Ö, Tunalı Akbay T, Yanardağ R. Deuterium Ethics Committee Approval: None. concentrations of drinking waters in Turkey (in Turkish). DSI Tech Bull Informed Consent: None. 2016; 120: 35-40. [8] Goncharuk VV, Lapshin VB, Burdeinaya TN, Pleteneva TV, Peer-review: Externally peer-reviewed. Chernopyatko AS, Atamanenko ID, et al. Physicochemical properties Author contributions: Concept – A.Y., B.A.T.; Design – A.Y., B.A.T.; and biological activity of the water depleted of heavy isotopes. J Water Supervision – A.Y.; Materials – B.A.T., A.Y.; Data Collection and/or Chem Tech 2011; 33(1): 8-13. Processing – B.A.T., A.Y.; Analysis and/or Interpretation – B.A.T., [9] Mladin C, Popescu AL, Stefanescu I, Oubraham A. Deuterium depleted A.Y.; Literature Search B.A.T.; Writing – B.A.T., A.Y.; Critical Reviews water-new studies about isotopic distillation obtaining process. Asian J – A.Y., B.A.T. Chem 2013; 25(14):7976-7978. [10] Barishev MG, Dzhimak SS, Frolov VU, Bolotin SN, Dolgov MA. Conflict of Interest: None. Technologies for obtaining deuterium depleted water. Int J Eng Res Financial Disclosure: None. Appl 2013; 3(1):523-526. 227.
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