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Preparation of Saussurea Costus Traditional Oil and Investigation of Different Parameters for Standardization

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Preparation of Saussurea Costus Traditional Oil and Investigation of Different Parameters for Standardization Research Journal of Pharmacognosy 5(2), 2018: 51-56 Received: 30 Oct 2017 Accepted: 10 Mar 2018 Published online: 13 Mar 2018 DOI: 10.22127/RJP.2018.58507 Original article Preparation of Saussurea costus Traditional Oil and Investigation of Different Parameters for Standardization Sahar Bagheri1, Nastaran Ebadi1, Zahra Taghipour2, Azadeh Manayi3, Tayebeh Toliyat4, Mehran Mirabzadeh Ardakani1* 1Department of Traditional Pharmacy, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran. 2School of Pharmacy, Tehran University of Medical Science, Tehran, Iran. 3Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. 4Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Science, Tehran, Iran. Abstract Background and objective: Medicinal oils are one of the most common and special dosage forms in oral and topical therapies of Persian medicine (PM). The oil of Saussurea costus (bitter qust) root is prominent topical oil with different applications in PM. In this study, the oil of bitter qust was prepared according to ancient Persian medical texts. Methods: To prepare traditional qust oil, 100 g of the root was soaked in 600 mL aqueous ethanol 25% overnight. The supernatant was then filtered and boiled in 800 g sesame oil until all water was evaporated. The essential oil of the root and volatile components of its traditional oil were extracted using hydro-distillation method in a Clevenger-type apparatus and were analyzed by gas chromatography-mass spectrometry (GC-MS) method. Total phenolics, flavonoids, tannins and polysaccharides were determined by spectrophotometric methods to evaluate the chemical parameters of traditional bitter qust oil. Results: The content of volatile compounds in both investigated samples was determined (0.5% and 0.1% (v/w), respectively). Dehydrocostus lactone and 1, 3-cyclooctadiene were two similar main compounds in the both analyzed samples. Total phenolics (788.290±0.61 mg/L gallic acid equivalent (GAE)), flavonoids (303.2±2.52 mg/L catechin equivalent (CE)), tannins (23.97±0.52 mg/L GAE) and polysaccharides (9.240±0.13 mg/L dextrose equivalent (DE)) contents were determined. Conclusion: According to the obtained data, dehydrocostus lactone could be used for determination and evaluation of traditional bitter qust oil. Keywords: bitter qust oil; costus root; Persian medicine; Saussurea costus; traditional oil Citation: Bagheri S, Ebadi N, Taghipour Z, Manayi A, Toliyat T, Mirabzadeh Ardakani M. Preparation of Saussurea costus traditional oil and investigation of different parameters for standardization. Res J Pharmacogn. 2018; 5(2): 51-56 Introduction Oils are one of the oldest dosage forms in ancient disease prevention in the first step and treatment medical systems such as Persian medicine (PM) of different disorders at the next stage [4]. [1,2]. Persian medicine is a prominent, popular Medicinal herbs are mainly recruited in PM to and historical medicinal system similar to other treat diseases [5] while oils are one of the most various traditional systems of medicine like common preparations [6]. Various topical and Chinese medicine, Ayurveda and Homeopathy systematic applications of herbal oils have been [3]. PM encompasses two fundamental parts introduced in PM medical texts [5,7]. Traditional *Corresponding author: [email protected] © 2018. Open access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) Bagheri S. et al. oils are called ''Adhan'' (singular form: “Dohn”) Herbarium of Faculty of Pharmacy, Tehran in pharmaceutical books of PM and their University of Medical Sciences, Tehran, Iran therapeutic usage and preparation procedures with the voucher number of PMP-240. have been explained in detail [8]. More than thirty species of plants have been Chemicals introduced in PM to prepare herbal oils. These All chemical substances were of analytical grade drugs were administered in various diseases and obtained from Merck Company (Germany). especially arthritis, sciatica and muscle aches [9]. Traditional oils are divided into two categories; Preparation of TQO those which are taken directly from the oily parts To prepare TQO, 100 g of plant's root coarse of the plants (sesame seed, olive fruits) and the powder was soaked overnight in 600 mL aqueous others obtained from extraction of non-oily parts ethanol 25%. The supernatant was then filtered of the plants in oil vehicle that causes trapping of using whatman filter paper (No.1, Sigma- hydrophobic and hydrophilic agents in the Aldrich, Germany) and boiled in 800 g sesame vehicle [1,2,10,11]. The mentioned oils are both oil (Omid Company, Iran) until all water was categorized as fixed oil. Saussurea costus (Falc.) evaporated [5,6]. Lipsch. (bitter qust ) oil is an example of the second mentioned category that has different Extraction and analysis of essential oils therapeutic applications in the traditional The oil from the powdered roots of the plant (100 medicine of Iran and other countries such as g) and TQO (100 mL) were extracted using India and China [12]. hydro-distillation method in a Clevenger type Saussurea costus, is one of the main species of apparatus for 4 h at room temperature. The the genus Sausssurea (Asteraceae family) [13]. obtained volatile fractions were separately The reported active ingredients of this well- collected and dried using sodium sulphate known medicinal plant are mainly terpenes, while anhydrous [17]. different amounts of flavonoids, anthraquinones, alkaloids, tannins and inulin were reported in the Gas chromatography-mass spectrometry (GC- previous studies from the plant [14-16]. MS) Sesquiterpene lactones, such as custonolide and The volatile compounds were analyzed using an dehydrocostus lactone are the major components Agilent Technologies Gas chromatography of S. costus with several pharmacologic effects device connected to Mass system (Agilent, USA) including anti-inflammatory, antiulcer, anti- with DB-5 fused silica column (30 m×0.25 mm cancer and hepatoprotective activities which were i.d., film thickness 0.25 µm). The oven demonstrated in the various experiments [14]. temperature was held at 50 ºC for 5 min and The present study has aimed to prepare raised to 280 °C at a rate of 10°C/min. Helium traditional bitter qust oil (TQO) based on was used as the carrier gas at a flow rate of 1 traditional methods of Persian medicine and mL/min. The injector and detector temperatures determine some active ingredients of the oil to were 280 ºC. Ion source temperature was 150 °C conduct the oil standardization. Further, chemical and scan mass range of m/z was 50-550.The composition of essential oil of the plants root was compounds were identified by comparison of analyzed, since relatively non-polar constituents of the essential oil could trap in the fixed oil their mass spectra with the Wiley libraries and during preparation of TQO. As it has been retention indices with those reported in the mentioned before [1,2,11], other hydrophilic literature. compounds like phenol, tannins, flavonoids and polysaccharides may be extracted in traditional Methanol extraction of the TQO oils, therefore these compounds were detected in The methanol extraction of TQO was needed for the TQO. further experiments. TQO (10 mL) was mixed with methanol (10 mL) and after complete Material and Methods mixing, the methanol phase was separated by Plant material decantation funnel, the procedure was repeated Roots of S. costus were purchased from herbal three times to optimize extraction. The obtained market (Tehran, Iran, 2016) and identified at the methanol phase was then centrifuged for 8 min at 52 Res J Pharmacogn 5(2): 51-56 Saussurea costus traditional oil 4000 rpm (Megafuge 1.0, Germany) to remove polyvinyl poly pyrrolidone (100 mg), and oil droplets and the remained methanol was distilled water (1 mL). The mixture was removed using rotary evaporator (Heidolph, refrigerated for 15 min at 4 °C. Then it was Germany). The extraction yield was 1.1% and the centrifuged for 10 min at 3000 rpm to precipitate concentration of 1 mg/mL in methanol was used tannins. The supernatant (0.2 mL) was mixed for analysis. with distilled water (0.3 mL), Folin-Ciocalteu (0.25 mL), sodium bicarbonate (1.25 mL) and the Quantification of total flavonoids content absorbance of sample was measured at 725 nm Determination of total flavonoids was based on after 40 min. The total tannins content was complex creation by the AlCl3 in a methanol estimated with subtracting the first and second medium [18]. For the measurement, sodium absorption values. nitrate (0.15 mL, 5% in water) and AlCl3 (0.15 mL, 10% in methanol) were added to 1 mL of the Statistical analysis methanol extract of TQO and standard solutions All tests were performed in triplicate and the data of catechin (50, 75, 125, 150 µg/mL). Sodium were reported as mean±standard deviation (SD). hydroxide 4% (2 mL) was then added to the experimental system after 6 min and the volume Results and Discussion reached to 5 mL with distilled water. Eventually, The TQO of the S. costus root was successfully passing 15 min, the absorbance was measured at prepared from aqueous ethanol (25%) extract of 510 nm by UV-visible spectrophotometer the plant regarding the Persian medicine (Camag, Korea). instructions. According to the Persian traditional text, by evaporation of the aqueous phase, the Quantification of total polysaccharide content constituents of the extract would be trapped in Inulin is a primary storage polysaccharide in the the sesame oil (vehicle) [1]. The prepared TQO roots of Asteraceae members and some of was subjected to essential oil extraction which monocotyledons [16]. Saussurea costus is one of yielded 0.1% v/w volatile oil. The essential oil of the sources of inulin therefore, polysaccharide S. costus roots was subsequently extracted to determination is a useful test for the TQO.
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