Bioscience Reports (2019) 39 BSR20182214 https://doi.org/10.1042/BSR20182214 Research Article Elevated microRNA-145 inhibits the development of oral squamous cell carcinoma through inactivating ERK/MAPK signaling pathway by down-regulating HOXA1 Downloaded from http://portlandpress.com/bioscirep/article-pdf/39/6/BSR20182214/846268/bsr-2018-2214.pdf by guest on 01 October 2021 Junhai Ding1,*, Dubin Sun2,* and Pengfeng Xie3 1Department of Stomatology, Affiliated Hospital of Jining Medical University, Jining 272100, P.R. China; 2Department of Stomatology, Zoucheng People’s Hospital, Zoucheng 273500, P.R. China; 3Special Functions Section, Jinan Stomatological Hospital, Jinan 250001, P.R. China Correspondence: Pengfeng Xie (
[email protected]) Background: Oral cancer is one of the most frequent solid cancers worldwide, and oral squamous cell carcinoma (OSCC) constitutes approximately 90% of oral cancers. The dis- covery of reliable prognostic indicators would be a potential strategy for OSCC treatment. In the present study, we aim to explore the underlying mechanism by which microRNA-145 (miR-145) affected OSCC. Methods: Forty-eight patients diagnosed with OSCC were en- rolled to obtain the OSCC tissues and adjacent normal tissues. The targeting relationship between miR-145 and Homeobox A1 (HOXA1) was verified. In order to assess the effects of miR-145 in OSCC and the detailed regulatory mechanism, the SCC-9 cell line was adopted, in which expression of miR-145 and HOXA1 were altered by transfection. Then, a series of in vitro and in vivo experiments were performed to evaluate the cell viability, migration, in- vasion, and tumor growth. Results: miR-145 was poorly expressed and HOXA1 was highly expressed in OSCC.