Molecular and Functional Characterization of a Fads2 Orthologue in the Amazonian Teleost, Arapaima Gigas
Comparative Biochemistry and Physiology, Part B 203 (2017) 84–91 Contents lists available at ScienceDirect Comparative Biochemistry and Physiology, Part B journal homepage: www.elsevier.com/locate/cbpb Molecular and functional characterization of a fads2 orthologue in the Amazonian teleost, Arapaima gigas Mónica Lopes-Marques a,b, Rodrigo Ozório a,b, Ricardo Amaral c, Douglas R. Tocher d, Óscar Monroig d,⁎,1,L.FilipeC.Castroa,e,⁎,1 a CIIMAR – Interdisciplinary Centre of Marine and Environmental Research, U. Porto – University of Porto, Porto, Portugal b ICBAS - Institute of Biomedical Sciences Abel Salazar, U. Porto - University of Porto, Portugal c Universidade Federal do Acre, Brazil d Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, Scotland, UK e Department of Biology, Faculty of Sciences, U. Porto - University of Porto, Portugal article info abstract Article history: The Brazilian teleost Arapaima gigas is an iconic species of the Amazon. In recent years a significant effort has been Received 8 June 2016 put into the farming of arapaima to mitigate overfishing threats. However, little is known regarding the nutrition- Received in revised form 8 September 2016 al requirements of A. gigas in particular those for essential fatty acids including the long-chain polyunsaturated Accepted 27 September 2016 fatty acids (LC-PUFA) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The ability to biosynthesize Available online 29 September 2016 LC-PUFA is dependent upon the gene repertoire of fatty acyl desaturases (Fads) and elongases (Elovl), as well as their fatty acid specificities. In the present study we characterized both molecularly and functionally an Keywords: Arapaima gigas orthologue of the desaturase fatty acid desaturase 2 (fads2)fromA.
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