Electrochemical Analysis of Ki67 Protein As Pancreatic Cancer Biomarker Based on Graphene-Polydopamine Nanocomposite

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Electrochemical Analysis of Ki67 Protein As Pancreatic Cancer Biomarker Based on Graphene-Polydopamine Nanocomposite

Int. J. Electrochem. Sci., 12 (2017) 3040 – 3049 International Journal of ELECTROCHEMICAL SCIENCE www.electrochemsci.org Electrochemical Analysis of Ki67 Protein As Pancreatic Cancer Biomarker Based on Graphene-Polydopamine Nanocomposite Liguo Hao1,2*, Lijie Liu3, Xin Meng4, Hongsheng Cui4, Zixu Wang1 1 1.Medical Technology School of Qiqihar Medical University, No. 333 Bukui North Road, Jianhua District, Qiqihar City, Heilongjiang Province 161006, P.R. China 2 Academy of Medical Sciences of Qiqihar, No. 333 Bukui North Road, Jianhua District, Qiqihar City, Heilongjiang Province 161006, P.R. China 3 College of life Science and Agriculture Forestry, Qiqihar University, Qiqihar City, Heilongjiang Province 161006, P.R. China 4 Third Affiliated Hospital of Qiqihar Medical University, Qiqihar City, Heilongjiang Province 161006, P.R. China *E-mail: [email protected] doi: 10.20964/2017.04.66 Received: 24 January 2017 / Accepted: 3 March 2017 / Published: 12 March 2017 An electrochemical immunosensor was studied for sensitive detection of Ki-67 protein based on a dual amplification mechanism resulting from Au nanoparticles (AuNP)−polydopamine (PDA) as the sensor platform. By utilizing PDA, biomolecules was immobilized for both the construction of the sensor platform and the signal labeling. The particular platform of AuNP-PDA, as well as synthesizing horseradish peroxidase (HRP)-antibody (Ab2) functionalized AuNP-PDA@graphene was utilized to immensely enhance the sensitivity. The amperometry determination was used to obtain a linear response range of Ki-67 which was from 4 to 800 pg/mL, along with a very low detection limit (1.7 pg/mL) Keywords: Pancreatic neuroendocrine tumor; Ki-67; Immunosensor; Polydopamine; Graphene FULL TEXT © 2017 The Authors. Published by ESG (www.electrochemsci.org). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). .

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