Supplementary Information

Table S1. Phylum level composition of bacterial communities in eight New Brunswick sediments. Phylum IB * IB% 2B* 2B% 3B * 3B% 4B * 4B% 5B * 5B% 6B * 6B% 7B * 7B% 8B * 8B% 270 4.0 248 5.3 383 6.4 81 1.0 63 1.4 73 1.4 474 7.5 542 8.0 111 2.4 181 3.0 168 2.7 1882 27.7 1133 24.4 1196 20.1 1645 20.9 879 20.1 990 18.7 1450 39.5 2012 32.0 Chlorobi 54 1.2 66 1.2 88 1.3 2284 33.6 1851 39.9 2468 41.4 4349 55.4 2430 55.4 3020 56.9 1523 41.5 2510 39.9 307 4.5 133 3.6 65 1.0 Unclassified 1367 20.1 1205 26.0 1581 26.5 1474 18.8 824 18.8 1058 19.9 487 13.3 926 14.7 “Rare Phyla” 51 0.8 95 2.0 148 2.5 223 2.8 133 3.0 99 1.9 79 2.2 128 2.0 Total 6791 100.0 4643 100.0 5957 100.0 7856 100.0 4383 100.0 5306 100.0 3672 100.0 6283 100.0 * Denotes number of sequences.

Table S2. Number of sequences represented in the “Rare Phyla” group presented in Table S1. Phylum IB 2B 3B 4B 5B 6B 7B 8B Acidobacteria 5 Actinobacteria 62 39 33 13 1 9 Chlorobi 13 2 5 5 3 4 4 12 27 8 6 20 3 1 1 1 9 1 1 9 Lentisphaerae 4 2 2 2 12 4 Nitrospira 5 1 22 2 36 Planctomycetes 37 55 52 18 21 9 28 2 1 Verrucomicrobia 46 51 73 33 26

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Table S2. Cont. Cyanobacteriachloroplast 7 12 2 4 16 9 9 22 10 OD1 2 1 3 TM7 2 1 1 4 WS3 1 3 3 2 1 Total 51 95 148 223 133 99 79 128

Table S3. Family level taxonomic composition with in the Order . FamilywithintheOrder 1B * 1B% 2B * 2B% 3B * 3B% 4B * 4B% 5B * 5B% 6B * 6B% 7B * 7B% 8B * 8B% Actinomycetales Cellulomonadaceae 4.0 1.0 1.0 2.0 6.0 40.0 Demequinaceae 17.0 4.2 2.0 4.0 1.0 2.3 Geodermatophilaceae 4.0 1.0 1.0 2.0 4.0 3.8 2.0 4.5 Intrasporangiaceae 10.0 2.5 1.0 2.0 3.0 6.8 Kineosporiaceae 2.0 4.5 Microbacteriaceae 116.0 28.9 3.0 6.0 14.0 13.2 6.0 13.6 Micrococcaceae 7.0 1.7 3.0 6.0 6.0 5.7 1.0 6.7 1.0 2.3 Nakamurellaceae 1.0 2.0 Nocardioidaceae 122.0 30.3 9.0 18.0 31.0 29.2 6.0 40.0 16.0 36.4 Propionibacteriaceae 5.0 1.2 2.0 1.9 Pseudonocardiaceae 7.0 1.7 6.0 1.5 UnclassifiedActinomycetales 100.0 24.9 29.0 58.0 47.0 44.3 2.0 13.3 5.0 100.0 3.0 100.0 1.0 100.0 13.0 29.5 “RareFamily” 4.0a 1.0 2.0b 1.9 Total 402.0 100.0 50.0 100.0 106.0 100.0 15.0 100.0 5.0 100.0 3.0 100.0 1.0 100.0 44.0 100.0 * Denotes number of sequences; a 1B rare family: 1 Micromonosporaceae, 1 Kineosporiaceae, 1 Nakamurellaceae, 1 Nocardiaceae; b 3B rare family: 1 Pseudonocardiaceae, 1 Intrasporangiaceae. Mar. Drugs 2014, 12 3

Table S4. Closest GenBank match of sequence groups (>99% sequence identity) and unique isolates. * Isolate name in brackets indicates the reference isolate from each sequence group that was deposited in GenBank.

Sequence Sequence Number Closest GenBank Match % Query Genbank accession Group/Strain length % Identity of strains (Accession number) coverage number * Designation (bp) 1 123 508 griseus strain ABRIINW 13 16S ribosomal RNA gene, partial sequence (HQ597007.1) 100 89 KF646049 (6BA6) 2 2 461 Streptomyces sp. 220297 16S ribosomal RNA gene, partial sequence (GU130099.1) 99 98 KF646050 (1BA12) 3 5 462 Streptomyces caeruleus strain NRRL 2449 16S ribosomal RNA gene, partial sequence (EF178698.1) 98 99 KF646051 (3BD4) 4 19 458 Streptomyces sp. SXY49 16S ribosomal RNA gene, partial sequence (GU045532.1) 99 98 KF646052 (4BD8) 5 4 454 Streptomyces azureus strain NRRL B-2655 16S ribosomal RNA gene, partial sequence (NR_044136.1) 99 99 KF646053 (1BB9) 6 53 465 Streptomyces albidoflavus strain HD-109 16S ribosomal RNA gene, partial sequence (EF620361.1) 99 97 KF646054 (5BC6) 7 7 459 Streptomyces sp. SXY124 16S ribosomal RNA gene, partial sequence (GU045539.1) 99 98 KF646055 (7BF5) 8 13 458 Streptomyces drozdowiczii partial 16S rRNA gene, isolate PhyCEm-1349 (AM921646.1) 99 98 KF646056 (3BC11) 9 20 454 Streptomyces baarnensis strain NRRL B-1902 16S ribosomal RNA gene, partial sequence (EF178688.1) 99 100 KF646059 (2BA6) 10 7 451 Streptomyces sp. SirexAA-E, complete genome (CP002993.1) 99 99 KF646079 (6BC12) 11 2 844 Streptomyces sp. BK178 partial 16S rRNA gene, strain BK178 (FR692110.1) 99 99 KF646064 (3BG7) 12 7 453 Streptomyces albidoflavus strain HD-109 16S ribosomal RNA gene, partial sequence (EF620361.1) 99 99 KF646067 (4BC6) 13 11 459 Streptomyces sp. SirexAA-E, complete genome (CP002993.1) 99 97 KF646085 (7BF12) 14 2 455 Streptomyces lavendulae strain NRRL B-1230 16S ribosomal RNA gene, partial sequence (HQ426713.1) 99 100 KF646063 (3BG2) 15 5 449 Streptomyces sp. HB108 16S ribosomal RNA gene, partial sequence (GU213496.1) 99 98 KF646070 (5BA5) 16 5 454 Streptomyces flavogriseus partial 16S rRNA gene, type strain CBS 101.34T (AJ494864.1) 99 98 KF646061 (2BG3) 17 4 449 Streptomyces drozdowiczii partial 16S rRNA gene, isolate PhyCEm-1258 (AM921645.1) 100 99 KF646075 (5BC2) 18 3 453 Streptomyces sp. AKB-2008-ET11 partial 16S rRNA gene, strain AKB-2008-ET11 (AM988883.1) 99 99 KF646078 (6BC5) 19 12 458 Streptomyces sp. HBUM80670 16S ribosomal RNA gene, partial sequence (EU119202.1) 99 98 KF646076 (6BA9) 20 5 448 Streptomyces drozdowiczii partial 16S rRNA gene, isolate PhyCEm-1258 (AM921645.1) 99 100 KF646065 (4BC2)

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Table S4. Cont.

21 2 875 Streptomyces caeruleus strain NRRL 2449 16S ribosomal RNA gene, partial sequence (EF178698.1) 98 99 KF646068 (4BD5) 22 2 446 Streptomyces sp. DSM 21069 16S ribosomal RNA gene, partial sequence (FJ872524.1) 99 100 KF646069 (4BD7) 23 6 454 Streptomyces sp. ME02-6978.2d 16S ribosomal RNA gene, complete sequence (EU080941.1) 99 98 KF646060 (2BB1) 24 3 449 Streptomyces phaeochromogenes strain NRRL B-2031 16S ribosomal RNA gene, partial sequence 98 100 KF646082 (7BE9) 25 2 418 Streptomyces peucetius gene for 16S rRNA, partial sequence (AB249907.1) 99 100 KF646071 (5BA11) 26 11 451 Streptomyces sp. HB116 16S ribosomal RNA gene, partial sequence (GU213500.1) 100 99 KF646083 (7BE12) 27 2 455 Streptomyces sp. SirexAA-E, complete genome (CP002993.1) 99 98 KF646074 (5BC1) 28 3 452 Streptomyces sp. VTT E-99-1326 (A4) 16S ribosomal RNA gene, partial sequence (AF429390.1) 100 99 KF646077 (6BB7) 29 14 456 Streptomyces bottropensis strain cfcc3173 16S ribosomal RNA gene, partial sequence (GQ258687.1) 99 99 KF646058 (2BA2) 30 2 447 Streptomyces globosus strain 12620-1 16S ribosomal RNA gene, partial sequence (EF371433.1) 99 100 KF646081 (7BE6) 31 6 461 Streptomyces sp. AKB-2008-ET11 partial 16S rRNA gene, strain AKB-2008-ET11 (AM988883.1) 99 98 KF646087 (8BC10) 32 4 450 Streptomyces caeruleus strain NRRL 2449 16S ribosomal RNA gene, partial sequence (EF178698.1) 99 98 KF646072 (5BB1) 33 6 517 Streptomyces sp. 219840 16S ribosomal RNA gene, partial sequence (HQ992732.1) 99 87 KF646086 (8BA6) 34 7 456 Streptomyces sp. 219840 16S ribosomal RNA gene, partial sequence (HQ992732.1) 99 99 KF646057 (1BD9) 35 9 463 Streptomyces griseus strain ABRIINW 13 16S ribosomal RNA gene, partial sequence (HQ597007.1) 99 98 KF646062 (3BD11) 36 20 544 Streptomyces sp. ME01-21h 16S ribosomal RNA gene, complete sequence (EU080953.1) 100 82 KF646073 (5BB10) 37 4 439 Nocardiopsis dassonvillei subsp. dassonvillei DSM 43111 chromosome 2, complete sequence (CP002041.1) 99 100 KF646066 (4BC3) Streptomyces peucetius strain JCM 9920 16S ribosomal RNA, 38 3 450 99 96 KF646080 (7BB10) partial sequence >dbj|AB045887.1| Streptomyces peucetius gene for 16S Rrna 39 2 510 Streptomyces flavogriseus ATCC 33331, complete genome (CP002475.1) 100 86 KF646084 (7BF2) 1BA3 1 452 Streptomyces tritolerans strain DAS 165 16S ribosomal RNA gene, partial sequence (DQ345779.2) 99 100 KF646009 1BB1 1 1515 Streptomyces sp. ME01-21h 16S ribosomal RNA gene, complete sequence (EU080953.1) 99 100 KF646008 1BC7 1 450 Streptomyces sp. HB110 16S ribosomal RNA gene, partial sequence (GU213497.1) 99 99 KF646001

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Table S4. Cont.

1BD6 1 461 Streptomyces sp. AKB-2008-ET11 partial 16S rRNA gene, strain AKB-2008-ET11 (AM988883.1) 99 98 KF646002 1BE1 1 438 Streptomyces griseus strain ABRIINW 13 16S ribosomal RNA gene, partial sequence (HQ597007.1) 98 100 KF646003 1BE3 1 461 Streptomyces albidoflavus strain HD-109 16S ribosomal RNA gene, partial sequence (EF620361.1) 98 98 KF646004 1BE5 1 435 Streptomyces sp. HB184 16S ribosomal RNA gene, partial sequence (GQ863916.1) 99 100 KF646005 1BE11 1 455 Streptomyces sp. ME01-21h 16S ribosomal RNA gene, complete sequence (EU080953.1) 99 99 KF646006 2BA12 1 456 Streptomyces bikiniensis strain DSM 40581 16S ribosomal RNA, partial (NR_026177.1) 98 98 KF646007 2BB11 1 446 Streptomyces albidoflavus strain HD-109 16S ribosomal RNA gene, partial (EF620361.1) 99 98 KF646010 2BC11 1 443 Streptomyces badius strain NRRL B-2567 16S ribosomal RNA, partial sequence (NR_043350.1) 98 97 KF646011 2BD2 1 318 Streptomyces sp. JJB-6 16S ribosomal RNA gene, partial sequence (GU132497.1) 99 100 KF646012 2BD3 1 320 Streptomyces flavogriseus ATCC 33331, complete genome (CP002475.1) 98 100 KF646013 2BD11 1 386 Streptomyces violascens strain S11A-1 16S ribosomal RNA gene, partial (HQ238294.1) 99 100 KF646014 2BE8 1 453 Streptomyces sp. ME01-21h 16S ribosomal RNA gene, complete sequence (EU080953.1) 99 98 KF646015 2BF1 1 448 Streptomyces sp. MI-BOB partial 16S rRNA gene, isolate MI-BOB (FN550147.1) 99 99 KF646016 2BMA3 1 361 Nocardiopsis sp. TFS72-17 16S ribosomal RNA gene, partial sequence (HM001279.1) 99 100 KF646017 3BA6 1 455 Streptomyces parvus partial 16S rRNA gene, strain moss18, isolate (FR846235.1) 99 98 KF646018 3BA9 1 453 Streptomyces sp. 220297 16S ribosomal RNA gene, partial sequence (GU130099.1) 99 99 KF646019 3BA11 1 364 Streptomyces violascens strain S11A-1 16S ribosomal RNA gene, partial (HQ238294.1) 99 100 KF646020 3BB11 1 366 Streptomyces sp. KLBMP 1072 16S ribosomal RNA gene, partial sequence (HM153776.1) 99 100 KF646021 3BD2 1 446 Streptomyces flavogriseus partial 16S rRNA gene, strain 10, clone chit_5 (FR832623.1) 99 100 KF646022 3BD3 1 381 Streptomyces sp. TN256 partial 16S rRNA gene, strain TN256 (FN687758.1) 99 100 KF646023 3BD8 1 456 Streptomyces sp. AKB-2008-LO6 partial 16S rRNA gene, strain AKB- (AM988888.1) 99 96 KF646024 3BD10 1 448 Streptomyces drozdowiczii strain NRRL B-24297 16S ribosomal RNA gene (EF654097.1) 99 99 KF646025 3BE9 1 451 Streptomyces subrutilus strain 173270 16S ribosomal RNA gene, partial (EU570663.1) 99 98 KF646026 3BF2 1 444 Promicromonospora sp. ME02-6983B 16S ribosomal RNA gene, complete (EU080946.1) 99 100 KF646027 3BF6 1 1491 Streptomyces sp. VTT E-99-1335 (B323) 16S ribosomal RNA gene, partial sequence (AF429399.1) 99 100 KF646028 3BG8 1 376 Actinoalloteichus sp. HB272 16S ribosomal RNA gene, partial sequence (GQ863923.1) 99 99 KF646029 3BG9 1 446 Streptomyces sp. SXY49 16S ribosomal RNA gene, partial sequence (GU045532.1) 99 99 KF646030 4BA12 1 455 Streptomyces sp. AKB-2008-ET11 partial 16S rRNA gene, strain AKB (AM988883.1) 99 98 KF646031

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Table S4. Cont.

4BB1 1 447 Streptomyces flavogriseus partial 16S rRNA gene, strain 10, clone chit_5 (FR832623.1) 99 100 KF646032 4BB9 1 450 Streptomyces albus subsp. albus gene for 16S Rrna (AB045884.1) 99 97 KF646033 4BD1 1 375 Streptomyces violascens strain G8A-24 16S ribosomal RNA gene, partial sequence (HQ238409.1) 99 100 KF646034 5BA3 1 1494 Streptomyces sp. VTT E-99-1336 (B329) 16S ribosomal RNA gene, partial sequence (AF429400.1) 99 100 KF646035 5BB4 1 447 Streptomyces sp. SXY83 16S ribosomal RNA gene, partial sequence (GU045536.1) 99 100 KF646036 5BC4 1 407 Streptomyces griseus strain ABRIINW 13 16S ribosomal RNA gene, partial (HQ597007.1) 99 100 KF646037 5BMB1 1 514 Promicromonospora sp. ME02-6983B 16S ribosomal RNA gene, complete sequence (EU080946.1) 99 87 KF646038 6BA10 1 436 Streptomyces griseus strain DSM 40931 16S ribosomal RNA gene, partial (GU383217.1) 99 99 KF646039 6BC4 1 447 Streptomyces flavogriseus partial 16S rRNA gene, strain 10, clone chit (FR832623.1) 99 100 KF646040 6BMA7 1 401 Streptomyces sp. HB110 16S ribosomal RNA gene, partial sequence (GU213497.1) 99 100 KF646041 7BA11 1 441 Streptomyces sp. HB132 16S ribosomal RNA gene, partial sequence (GQ863908.1) 98 100 KF646042 7BB12 1 448 Streptomyces sp. ME02-6979.3a 16S ribosomal RNA gene, complete (EU080943.1) 99 98 KF646043 7BC7 1 440 Streptomyces sp. L083 16S ribosomal RNA gene, partial sequence (EU135902.1) 99 99 KF646044 7BD3 1 382 Streptomyces sp. MS218 16S ribosomal RNA gene, partial sequence (EU908199.1) 98 100 KF646045 7BD4 1 509 Streptomyces luridiscabiei strain S63 16S ribosomal RNA, complete sequence (NR_025155.1) 100 87 KF646000 8BA1 1 459 Streptomyces albidoflavus strain HD-109 16S ribosomal RNA gene, partial (EF620361.1) 99 99 KF646046 8BC4 1 458 Streptomyces sp. MS1/7 16S ribosomal RNA gene, partial sequence (AY550275.2) 98 99 KF646047 8BC6 1 452 Streptomyces drozdowiczii partial 16S Rrna gene, isolate PhyCEm-1258 (AM921645.1) 99 99 KF646048

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Table S5. Composition of sequence groups consisting of strains which share >99% 16S rRNA sequence identity.

Sequence Number of Strains in sequence group group strains Isolate location denoted by first two characters of strain name 1BA1, 1BA4, 1BA5, 1BA6, 1BA7, 1BA9, 1BA11, 1BB4, 1BB5, 1BB6, 1BB10, 1BB11, 1BC11, 1BC2, 1BC3, 1BC5, 1BD1, 1BD7, 1BD10, 1BE7, 1BF1, 2BA4, 2BA5, 2BA7, 2BA10, 2BB4, 2BB6, 2BB12, 2BC2, 2BC3, 2BC7, 2BC8, 2BC12, 2BD4, 2BD9, 2BD10, 2BE2, 2BE3, 2BE4, 2BE5, 2BE7, 2BE9, 2BF3, 2BF5, 2BF8, 2BF9, 2BF12, 2BG1, 3BA1, 3BA4, 3BA12, 3BB7, 3BB8, 3BC3, 3BC4, 3BD1, 3BE2, 3BE3, 3BE4, 3BE5, 3BE11, 3BF1, 3BF3, 3BF5, 3BG3, 3BG6, 3BF10, 4BA5, 1 123 4BA9, 4BB2, 4BB12, 4BC4, 4BC5, 4BC7, 4BC8, 4BC10, 4BD3, 4BD4, 4BD9, 5BA8, 5BA10, 5BB7, 5BC5, 5BC7, 5BC9, 5BD2, 5BD5, 5BD6, 5BD7, 6BA6, 6BB2, 6BC1, 6BC2, 6BC10, 6BC11, 6BMA8, 7BB1, 7BC5, 7BC9, 7BC12, 7BD5, 7BD6, 7BD8, 7BD11, 7BD12, 7BE3, 7BE4, 7BE11, 7BF4, 7BF6, 7BF10, 7BG3, 7BG4, 7BG10, 7BMA9, 8BA3, 8BA4, 8BA5, 8BA11, 8BB11, 8BB12, 8BC2, 8BC3 2 2 1BA12, 1BD8 3 5 1BA2, 1BA8, 3BD4, 7BF3, 8BB5 4 19 1BB12, 1BE10, 2BA1, 2BA8, 3BA5, 3BC7, 3BG10, 4BA7, 4BA11, 4BD10, 4BD8, 5BA2, 5BB8, 5BB11, 6BA1, 6BC7, 7BG6, 8BB1, 8BB4 5 4 1BB7, 1BB9, 7BC8, 7BG8 1BA10, 1BB8, 1BC4, 1BC6, 1BC9, 1BD12, 1BE2, 1BE4, 2BA9, 2BA11, 2BB8, 2BC9, 2BD8, 2BD12, 2BE1, 2BE10, 2BE12, 2BF10, 3BA3, 3BA7, 3BA8, 3BB5, 6 53 3BB9, 3BB10, 3BC6, 3BC12, 3BD5, 4BA6, 4BC11, 4BD6, 5BA4, 5BC6, 5BC10, 5BMA6, 6BA2, 6BA4, 6BA8, 6BB5, 6BC9, 7BA4, 7BA6, 7BA7, 7BB2, 7BB6, 7BD2, 7BF1, 7BF8, 7BF9, 7BG5, 7BG7, 8BA7, 8BA8, 8BC7 7 7 1BD4, 4BB10, 4BE1, 5BA12, 7BA8, 7BA9, 7BF5 8 13 1BB3, 1BD5, 3BB4, 3BC11, 3BE10, 4BB7, 4BC1, 4BE2, 5BB3, 5BC11, 5BD8, 6BB1, 6BC3 1BC8, 1BE6, 1BE8, 1BE12, 2BA6, 2BC1, 2BD1, 2BF7 9 20 3BG4, 4BB11, 5BA9, 5BB9, 6BB4, 6BC8, 7BA3, 7BB8, 7BC11, 7BD7, 7BE10, 7BG2 10 7 2BA3, 2BB2, 3BB2, 3BD12, 3BF12, 6BA11, 6BC12 11 2 2BB7, 3BG7 12 7 3BA10, 3BB3, 3BE1, 3BF9, 3BG5, 4BC6, 5BB2 13 11 2BC5, 2BD6, 2BF4, 3BA2, 3BB1, 3BB6, 3BB12, 4BA10, 4BD12, 5BC12, 7BF12 14 2 3BC5, 3BG2 15 5 3BC8, 4BD11, 5BA5, 6BB3, 6BB11 16 5 2BG3, 3BC9, 3BD6, 3BE12, 6BA5 17 4 3BD9, 3BE8, 5BB5, 5BC2 18 3 2BC4, 3BF8, 6BC5

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Table S5. Cont.

19 12 2BB10, 3BD7, 3BE7, 3BF7, 4BB5, 4BB6, 4BC12, 5BA7, 5BB12, 5BD1, 6BA9, 6BC6 20 5 4BC2, 6BA3, 6BA12, 6BB8, 6BB9 21 2 4BB8, 4BD5 22 2 1BMA2, 4BD7 23 6 2BB1, 2BE6, 3BF4, 4BA3, 5BA1, 5BC8 24 3 4BD2, 5BD4, 7BE9 25 2 5BA11, 6BA7 26 11 2BB3, 2BC6, 2BF6, 5BD3, 6BB12, 7BB7, 7BD10, 7BE2, 7BE7, 7BE12, 7BG1 27 2 5BC1, 6BB6 28 3 6BB7, 7BA1, 7BA12 29 14 2BA2, 2BB9, 3BE6, 4BA1, 4BA8, 5BA6, 5BC3, 6BB10, 7BA10, 7BB5, 7BB11, 7BC4, 7BC6, 7BC10 30 2 7BD9, 7BE6 31 6 1BD3, 8BA9, 8BB6, 8BB8, 8BC9, 8BC10 32 4 5BB1, 7BE8, 7BF11, 8BB10 33 6 3BC10, 7BC1, 7BE5, 7BD1, 8BA6, 8BB2 34 7 1BD9, 2BC10, 2BG2, 5BB6, 7BC2, 7BE1, 8BB3 35 9 1BB2, 3BC1, 3BC2, 3BD11, 8BA10, 8BB7, 8BB9, 8BC1, 8BC5 36 20 1BC12, 1BE9, 1BMA1, 2BB5, 2BD5, 2BF2, 2BE11, 3BG1, 3BMA4, 4BA4, 4BC9, 5BB10, 5BMB2, 7BA2, 7BA5, 7BB3, 7BB9, 7BG9, 8BA12, 8BC8 37 4 3BF11, 4BC3, 7BB4, 7BC3 38 3 4BB3, 4BB4, 7BB10 39 2 2BF11, 4BA2

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Figure S1. Canonical correspondence analysis (CCA) of the relationship between culture-independent level community composition

(in black: OTUs identified using mothur) and sediment parameters (in red: % Organic Matter (OM), pH, P2O2, K2O, Ca and Mg) by site (in green: Black Beach (1B, 2B, 3B), Chance Harbour (4B, 5B, 6B), Pocologan (7B) and Mispec Bay (8B)).

CCA Map / Symmetric (axes F1 and F2: 53.17 %) 3

2

Site 5B Site 6B 111312411473159320332073218528493153319332093313332136013625364936813705371337213729385738894025403340414049406540814089409741054113412141294137414541614169428142894297430543134329434543534361437743854393440144094417442544334441444944574465447344814489449745054513452145294537454545534561456945774585459346014609461746254633464146494657466546734681468946974705471347214737474547534761476947774785479348014809481748254833484148494857486548734881488948974905491320012057206526172625265727052721280928257371329197710172329136112968910971793180923452353264129773009301730653073405740734153417742014321433743694729591359535961596959775985599360016137614561536161616961776185619362016209621762256233624162496257626562736281628962976305631363216329633763456353636163696841918491897144915772731145136973228956929052969 Site 4B 2857286529212945295329853041309710731931531193137181718254332572305244163313135372415778091201155324092569266526732681271328972961299330013057588958975905592159295937594560096017602560336041604960576065607360816089609761056113612161296617662566336641664966576665667366816689669767056713672167296737674567536761677767856793680168096817682568336849 1 1 65161 108147335333937 401 17771649 841 361 265 13371633

%) 929 2017 785793 16894251569 593 1985 1665 5055851889 10332457 1841 Ph 211326492817 (24.00 134512891417287325453081 F2 321 17 1217 3305 1833 15457531385219315053113316932652577 32333881 0 1865 121 145 4891009 P2O2 1105 Site 7B 1657 3454572891353 889 913 281 905 Mg Ca 817 85717691745 104911611169118512651297140914251433146514811785180119292049208121052337241724652481248925052553256125933289375337613769379338093817383338413849386539053945395339613969398540014009492149294937494549694977498549935001500950175025503351695177518551935201520952175225523352415249525752655273528152895297530553135321532953375345535353615369537753855393540158736377638563936401640964176425643364416449645764656473648164896497650565136521652965376545655365616569657765856593660166096769169177225305441601617761873201 2321 649 8811993 12811025225723131177 745 449 16091913243324732521252925853801382539293937397739936873690569216961697769857001700971377161716972097273740974418137814581698177819382018241824982898297830583138353836183778385839384018409843384738481849785058521853785538561856985778585859386338649865786658689872187298769877787858841886588738881888989291057521673 8652097 K2O 172911291209 173770528012929303332973513352935373545377749215322092233 3121 18571137200960929721752957897 Site 2B 18731905 1089162525372609268928412889293730493105227314571225769641106513771529160119451969212121292145217722012377251326012633269727292737274527532761276927772785279328332881302531773873389741854193420942174225423342414249425742654273685768656881688968976913692969536969699370177025704170497057712171297153717772337241725772657281731373297337735373617369739374177433744974657473748175297553756175697585760976497753781778257857788979217937795379697985801780258057806580978105811381218161818582178225823382578265827383298337834583698417842584418457848985138529860186098617862586418673868186978713875387618793880188178825884988578897890589138921 1673168110417132491561291354510012497140115851753369 681 19619779854140999332919212025 Site 3B 1721825385481255616653281332933693489357737853913 96120920419219733734813633 ‐1 OM 132115211641185105113513657953161722492401892169 115312491393169717131881195321372217222522412265308931453185322532413257327333373393342534333441344934573465347334973553356135933665367336893697373737453921401769376945703370657073708170897097710571137145718571937201721772257249728972977305732173457377738574017425745774897497750575137521753775457577759376017617762576337641765776657673768176897697770577137721772977377745776177697777778577937801780978337841784978657873788178977905791379297945796179777993800180098033804180498073808180898129815382098281832184498465854587058737874588098833393697721811257553945 1937216132013617123313054652281833729127377741711211441148914971513153717051761208922972361236923852393242524493129313731613217324933453353336133773385340134093417350535213569358536093641365749534961504150495057506550735081508950975105511351215129513751455153516154095417542554335441544954575465547354815489549755055513552155295537554555535561556955775585559356015609561756255633564156495657566556735681568956975705571357215729573757455753576157695777578557935801580958175825583358415849585758655881625377313233497801849969 Site 1B Site 8B

‐2 ‐4 ‐3 ‐2 ‐1 0 1 2 3 4 5 F1 (29.17 %)

Sites Objects Variables

Mar. Drugs 2014, 12 10

Figure S2. Canonical correspondence analysis (CCA) of the relationship between culture-independent species level community composition (in black: OTUs identified using mothur) and the sediment parameters (in red: B, Cu, Fe, Na, Zn, S) by site (in green: Black Beach (1B, 2B, 3B), Chance Harbour (4B, 5B, 6B), Pocologan (7B) and Mispec Bay (8B)).

CCA Map / Symmetric (axes F1 and F2: 52.82 %)

2

1201 1193537577241633809 11459107373433137155324092569266526732681271328972961299330013057588958975905592159295937594560096017602560336041604960576065607360816089609761056113612161296617662566336641664966576665667366816689669767056713672167296737674567536761677767856793680168096817682568336849 109710175692731313 689290529697371292305244118171825257153 Site 6B 228918491897136928572865292129452953298530413097193937 1 17931809234523532641297730093017306530734057407341534177420143214337436947295913595359615969597759855993600161376145615361616169617761856193620162096217622562336241624962576265627362816289629763056313632163296337634563536361636968411113124113611449157713292329 1977200120572065261726252657270527212809282516133 Site 5B 1473159320332073218528493153319332093313332136013625364936813705371337213729385738894025403340414049406540814089409741054113412141294137414541614169428142894297430543134329434543534361437743854393440144094417442544334441444944574465447344814489449745054513452145294537454545534561456945774585459346014609461746254633464146494657466546734681468946974705471347214737474547534761476947774785479348014809481748254833484148494857486548734881488948974905491365353 401 473265 1 361 1081 793 841 Site 4B 593 16491569 785929 425 S 17771633 2457 5851889 20171689 1033 321 1337505 254530811217 1665 1985184117 2577 211326492817128914172873 1345150531133169326510091865 3305 Na 1833 1385 0 154521931353 32333881

753 1657 145 289 Site 7B 121 11051745 %) 489457 Fe 1769 12651297140914251433146514811785180119292049208121052337241724652481248925052553256125933289375337613769379338093817383338413849386539053945395339613969398540014009492149294937494549694977498549935001500950175025503351695177518551935201520952175225523352415249525752655273528152895297530553135321532953375345535353615369537753855393540158736377638563936401640964176425643364416449645764656473648164896497650565136521652965376545655365616569657765856593660166096769 889 913 B 281 345128122572313 905 232113771529160119451969212121292145217722012377251326012633269727292737274527532761276927772785279328332881302531773873389741854193420942174225423342414249425742654273685768656881688968976913692969536969699370177025704170497057712171297153717772337241725772657281731373297337735373617369739374177433744974657473748175297553756175697585760976497753781778257857788979217937795379697985801780258057806580978105811381218161818582178225823382578265827383298337834583698417842584418457848985138529860186098617862586418673868186978713875387618793880188178825884988578897890589138921 1993 817 10251177 1049116111691185169177225305441601617761873201 2097 857215322092233 (23.71 649 745 280129293033329735133529353735453777 Site 3B 881 3121 449 172952167310651401158517532025 865 160919132433247325212529258538013825392939373977399368736905692169616977698570017009713771617169720972737409744181378145816981778193820182418249828982978305831383538361837783858393840184098433847384818497850585218537855385618569857785858593863386498657866586898721872987698777878588418865887388818889892949145789724971921369 F2 Zn 10571129173712092009227364134813633 Site 2B 18731905 Cu 18572175291225571561291376932813329336934893577378539132169 70516252537260926892841288929373049310516731681100122492401 60929720413293371257 11371961249545152116419936651617 ‐1 108910411721414091321561979218912731441148914971513153717051761208922972361236923852393242524493129313731613217324933453353336133773385340134093417350535213569358536093641365749534961504150495057506550735081508950975105511351215129513751455153516154095417542554335441544954575465547354815489549755055513552155295537554555535561556955775585559356015609561756255633564156495657566556735681568956975705571357215729573757455753576157695777578557935801580958175825583358415849585758655881 Site 8B 681 713385481253201361713052281945625 9779851937216181553313 825209124913931697171318811953213722172225224122653089314531853225324132573273333733933425343334413449345734653473349735533561359336653673368936973737374539214017693769457033706570737081708970977105711371457185719372017217722572497289729773057321734573777385740174257457748974977505751375217537754575777593760176177625763376417657766576737681768976977705771377217729773777457761776977777785779378017809783378417849786578737881789779057913792979457961797779938001800980338041804980738081808981298153820982818321844984658545870587378745880988336579537774171121377233497801849969 Site 1B 9611055131851137211233465833729 1153393697

‐2

‐3 ‐5 ‐4 ‐3 ‐2 ‐1 0 1 2 3 4 5 F1 (29.12 %)

Sites Objects Variables

Mar. Drugs 2014, 12 11

Figure S3. Canonical correspondence analysis (CCA) of the relationship between culture-independent species level community composition (in black: OTUs identified using mothur) and the sediment parameters (in red: sediment particle size: size 1 (<0.6 mm), size 2 (0.6–1 mm), size 3 (1–2 mm) and size 4 (>2 mm)) by site (in green: Black Beach (1B, 2B, 3B), Chance Harbour (4B, 5B, 6B), Pocologan (7B) and Mispec Bay (8B)).

CCA Map / Symmetric (axes F1 and F2: 64.34 %)

3

2

Site 6B 1201155324092569266526732681271328972961299330013057588958975905592159295937594560096017602560336041604960576065607360816089609761056113612161296617662566336641664966576665667366816689669767056713672167296737674567536761677767856793680168096817682568336849739137 1 13138093318171825129 230524415375771241 10736334331193 28572865292129452953298530413097 25453081 Site 5B 2289290529691145136918491897 2731217 10172329 18891865

144915775692571361652656891329153 737 1353 106513771529160119451969212121292145217722012377251326012633269727292737274527532761276927772785279328332881302531773873389741854193420942174225423342414249425742654273685768656881688968976913692969536969699370177025704170497057712171297153717772337241725772657281731373297337735373617369739374177433744974657473748175297553756175697585760976497753781778257857788979217937795379697985801780258057806580978105811381218161818582178225823382578265827383298337834583698417842584418457848985138529860186098617862586418673868186978713875387618793880188178825884988578897890589138921 1505311331693265 10971793180923452353264129773009301730653073405740734153417742014321433743694729591359535961596959775985599360016137614561536161616961776185619362016209621762256233624162496257626562736281628962976305631363216329633763456353636163696841164919779373532617262526572705272128092825 193200120572065161 1081 184116891113124114731593203320732185284931533193320933133321360136253649368137053713372137293857388940254033404140494065408140894097410541134121412941374145416141694281428942974305431343294345435343614377438543934401440944174425443344414449445744654473448144894497450545134521452945374545455345614569457745854593460146094617462546334641464946574665467346814689469747054713472147374745475347614769477747854793480148094817482548334841484948574865487348814889489749054913 Site 3B

%) SIZE3

473 401321 1105 Site 4B 793 841361 1777585425 2457593 2017 215322092233897 929785 1417287316331985 1009289 1177 (26.81 1451569

2649281717505 14011585175313451337369 1665 28012929303332973513352935373545377749 F2 1033 219312091545 217128112251921 1833 172910251457 3305 745 67352976910012113 0 1745 SIZE2 1609191324332473252125292585380138253929393739773993687369056921696169776985700170097137716171697209727374097441813781458169817781938201824182498289829783058313835383618377838583938401840984338473848184978505852185378553856185698577858585938633864986578665868987218729876987778785884188658873888188898929857 2025 Site 7B 913 22733281332933693489357737853913 8891657 13856097531057 905 8811993 121 817 57 104911611169118512651297140914251433146514811785180119292049208121052337241724652481248925052553256125933289375337613769379338093817383338413849386539053945395339613969398540014009492149294937494549694977498549935001500950175025503351695177518551935201520952175225523352415249525752655273528152895297530553135321532953375345535353615369537753855393540158736377638563936401640964176425643364416449645764656473648164896497650565136521652965376545655365616569657765856593660166096769169177225305441601617761873201 281489 257717371289345545 32333881 Site 2B 649 45718575212497 641 11372971041993 11293852041 2097 108916252537260926892841288929373049310571348132934813633 SIZE1 2009 865 24915211641 449 16731681409665 18731905 196197798541132165722492401 5131617 17691561291317218252321 3121 SIZE4 705 681 961209953972169 ‐1 25185113721 56110522572313 Site 1B 1153124913931697171318811953213722172225224122653089314531853225324132573273333733933425343334413449345734653473349735533561359336653673368936973737374539214017693769457033706570737081708970977105711371457185719372017217722572497289729773057321734573777385740174257457748974977505751375217537754575777593760176177625763376417657766576737681768976977705771377217729773777457761776977777785779378017809783378417849786578737881789779057913792979457961797779938001800980338041804980738081808981298153820982818321844984658545870587378745880988333931257 921337 69789 81 19372161553945 32013617465 625 123313052281833729 Site 8B 1273777417313 11211441148914971513153717051761208922972361236923852393242524493129313731613217324933453353336133773385340134093417350535213569358536093641365749534961504150495057506550735081508950975105511351215129513751455153516154095417542554335441544954575465547354815489549755055513552155295537554555535561556955775585559356015609561756255633564156495657566556735681568956975705571357215729573757455753576157695777578557935801580958175825583358415849585758655881377233497801849969 ‐2 ‐4 ‐3 ‐2 ‐1 0 1 2 3 4 5 F1 (37.53 %)

Sites Objects Variables

Mar. Drugs 2014, 12 12

Figure S4. Canonical correspondence analysis (CCA) of the relationship between culture-dependent species level community composition

(in black: OTUs based on 99% sequence identity) and the sediment parameters (in red: % Organic Matter (OM), pH, P2O2, K2O, Ca and Mg) by site (in green: Black Beach (1B, 2B, 3B), Chance Harbour (4B, 5B, 6B), Pocologan (7B) and Mispec Bay (8B)).

CCA Map / Symmetric (axes F1 and F2: 45.93 %)

3

OTU14OTU57OTU58OTU59OTU60OTU61OTU62OTU63OTU64OTU65OTU66OTU67OTU68OTU69 2

OTU12

OTU11 1 OTU17 OTU21 OTU70OTU71OTU72OTU73 OTU13 Site 3B

%) OTU39OTU23 OTU16 Site 4B OTU38 OTU37 OTU35

(17.53 OTU19 OTU74OTU75OTU76OTU77OTU24 OTU8OTU48OTU49OTU50OTU51OTU52OTU53OTU54OTU55OTU56 F2 OTU22OTU4 OTU36 Site 5B Ph 0 Site 2B K2O OM OTU10 OTU1 OTU86OTU87OTU88 Mg OTU7Ca OTU33Site 8B OTU31 OTU29 OTU6 OTU3 OTU18 P2O2 OTU34 OUT32 OTU15 Site 7B OTU9Site 1B OTU26 OTU25OTU27 OTU2OTU40OTU41OTU42OTU43OTU44OTU45OTU46OTU47 Site 6B OTU5 ‐1 OTU30OTU81OUT82OTU83OTU84OTU85

OTU20 OTU28

OTU78OTU79OTU80

‐2 ‐4 ‐3 ‐2 ‐1 0 1 2 3 4 5 F1 (28.39 %)

Sites Objects Variables

Mar. Drugs 2014, 12 13

Figure S5. Canonical correspondence analysis (CCA) of the relationship between culture-dependent species level community composition (in black: OTUs based on 99% sequence identity) and the sediment parameters (in red: B, Cu, Fe, Na, Zn, S) by site (in green: Black Beach (1B, 2B, 3B), Chance Harbour (4B, 5B, 6B), Pocologan (7B) and Mispec Bay (8B)).

CCA Map / Symmetric (axes F1 and F2: 46.53 %)

4

2

OTU78OTU79OTU80OTU28 OTU30OTU81OUT82OTU83OTU84OTU85OTU5 OTU2OTU40OTU41OTU42OTU43OTU44OTU45OTU46OTU47 OTU20 Site 7B S Na OTU26 OTU9 OTU25OTU27 Fe Site 1B Site 6B OTU7OTU34 OTU29OTU22OTU6 OUT32 OTU3 %) OTU15 B

OTU18 OTU51 OTU1OTU48OTU49OTU50OTU52OTU53OTU54OTU55OTU56OTU36Site 2B 0 Zn Site 8B (18.02 Site 5B OTU33 OTU24Cu F2 OTU10 OTU8OTU37OTU4 OTU31 OTU38 OTU86OTU87OTU88 OTU19OTU74OTU75OTU76OTU77OTU39 Site 4B OTU23 OTU35 OTU16OTU21 OTU70OTU71OTU72OTU73 OTU13 OTU17 OTU11 Site 3B OTU12

‐2 OTU14OTU57OTU58OTU59OTU60OTU61OTU62OTU63OTU64OTU65OTU66OTU67OTU68OTU69

‐4 ‐10 ‐8 ‐6 ‐4 ‐2 0 2 4 6 8 10 F1 (28.51 %)

Sites Objects Variables

Mar. Drugs 2014, 12 14

Figure S6. Canonical correspondence analysis (CCA) of the relationship between culture-dependent species level community composition (in black: OTUs based on 99% sequence identity) and the sediment parameters (in red: sediment particle size: size 1 (<0.6 mm), size 2 (0.6–1 mm), size 3 (1–2 mm) and size 4 (>2 mm)) by site (in green: Black Beach (1B, 2B, 3B), Chance Harbour (4B, 5B, 6B), Pocologan (7B) and Mispec Bay (8B)).

CCA Map / Symmetric (axes F1 and F2: 64.46 %)

3

2

OTU86OTU87OTU88 OTU14OTU57OTU58OTU59OTU60OTU61OTU62OTU63OTU64OTU65OTU66OTU67OTU68OTU69 1 OTU78OTU79OTU80 OTU20 OTU35 OTU15OTU25OTU27OTU12 OTU31 OTU17 OTU16 %) OTU19 OTU74OTU75OTU76OTU77SIZE3 OTU18OTU10 OTU21OTU70OTU71OTU72OTU73 SIZE4 Site 8B Site 6B OTU8Site 3BOTU4

(25.40 OTU33 OTU23 OTU13 OUT32 F2 OTU24OTU11 Site 4B SiteOTU38 5B 0

OTU39 OTU28OTU29 OTU1 OTU6 OTU37 OTU36 Site 2B OTU34 OTU3 SIZE2 OTU7 OTU9 OTU26OTU22Site 7B OTU48OTU49OTU50OTU51OTU52OTU53OTU54OTU55OTU56 Site 1B ‐1 SIZE1

OTU30OTU81OUT82OTU83OTU84OTU85

OTU5

OTU2OTU40OTU41OTU42OTU43OTU44OTU45OTU46OTU47 ‐2 ‐4 ‐3 ‐2 ‐1 0 1 2 3 4 5 F1 (39.06 %)

Sites Objects Variables

© 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).