RGS14 (56-207) (Part# 8007) 6X N-Terminal His Tagged Human RGS14 (56-207) Expressed in E.Coli

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RGS14 (56-207) (Part# 8007) 6X N-terminal His tagged Human RGS14 (56-207) expressed in E.Coli Purpose: Measuring RGS14 enzymatic activity in a multiwell format Readout: Fluorescence polarization, far red (633/670 nm) Applications: Testing the effects of agents or treatments on RGS14 (56-207) enzymatic activity; e.g., inhibitor screening or dose response measurements. Enzyme info: Human RGS14 is a member of the Regulator of G protein signaling (RGS) family that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gαi, Gαo and Gαq alpha subtypes by driving G proteins into their inactive GDP-bound forms. His tagged RGS14 (56-207) contains the RGS domain and has been validated to work with the RGScreen™ platform. RGScreen GAP Assays: The most direct way to detect RGS function is by measuring the increased GTPase activity of the associated Gα protein. However, the GTPase activity of isolated Gα proteins is limited by GDP dissociation, so RGS GAP activity cannot be measured using simple biochemical assays. The RGScreen™ platform overcomes this kinetic constraint by using proprietary Gα protein variants with altered GTP hydrolysis and GDP dissociation rates that enable detection of RGS GAP activity using the Transcreener® GDP Assay. Assay info: GDP formed by RGS/Gα enzymatically is detected with a competitive fluorescence polarization immunoassay using a highly specific antibody for GDP. Related documents: Transcreener GDP FP Assay Technical Manual; Transcreener GDP FI Assay Technical Manual; Application Note: Direct Detection of RGS GAP Activity using the RGScreen™ Platform to Discover Small Molecule Inhibitors for RGS Proteins. Sample Data A 200 150 Gαi1(R178M,A326S) + RGS14 100 RGS14 GAP Enhancement mP Δ Gαi1(R178M,A326S) 50 0 0 60 120 180 Time(min) B GTPase Rate GAP Enhancement Gαi1(R178M,A326S) 0.0001 Gαi1(R178M,A326S) + RGS14 0.0006 6 .

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Supplemental Table B ARGs in alphabetical order Symbol Title 3 months 6 months 9 months 12 months 23 months ANOVA Direction Category 38597 septin 2 1557 ± 44 1555 ± 44 1579 ± 56 1655 ± 26 1691 ± 31 0.05219 up Intermediate 0610031j06rik kidney predominant protein NCU-G1 491 ± 6 504 ± 14 503 ± 11 527 ± 13 534 ± 12 0.04747 up Early Adult 1G5 vesicle-associated calmodulin-binding protein 662 ± 23 675 ± 17 629 ± 16 617 ± 20 583 ± 26 0.03129 down Intermediate A2m alpha-2-macroglobulin 262 ± 7 272 ± 8 244 ± 6 290 ± 7 353 ± 16 0.00000 up Midlife Aadat aminoadipate aminotransferase (synonym Kat2) 180 ± 5 201 ± 12 223 ± 7 244 ± 14 275 ± 7 0.00000 up Early Adult Abca2 ATP-binding cassette, sub-family A (ABC1), member 2 958 ± 28 1052 ± 58 1086 ± 36 1071 ± 44 1141 ± 41 0.05371 up Early Adult Abcb1a ATP-binding cassette, sub-family B (MDR/TAP), member 1A 136 ± 8 147 ± 6 147 ± 13 155 ± 9 185 ± 13 0.01272 up Midlife Acadl acetyl-Coenzyme A dehydrogenase, long-chain 423 ± 7 456 ± 11 478 ± 14 486 ± 13 512 ± 11 0.00003 up Early Adult Acadvl acyl-Coenzyme A dehydrogenase, very long chain 426 ± 14 414 ± 10 404 ± 13 411 ± 15 461 ± 10 0.01017 up Late Accn1 amiloride-sensitive cation channel 1, neuronal (degenerin) 242 ± 10 250 ± 9 237 ± 11 247 ± 14 212 ± 8 0.04972 down Late Actb actin, beta 12965 ± 310 13382 ± 170 13145 ± 273 13739 ± 303 14187 ± 269 0.01195 up Midlife Acvrinp1 activin receptor interacting protein 1 304 ± 18 285 ± 21 274 ± 13 297 ± 21 341 ± 14 0.03610 up Late Adk adenosine kinase 1828 ± 43 1920 ± 38 1922 ± 22 2048 ± 30 1949 ± 44 0.00797 up Early
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