DOCSLIB.ORG

Towards the Development of Soybean Resistance to Dectes

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Towards the Development of Soybean Resistance to Dectes Towards the development of soybean resistance to Dectes texanus LeConte (Coleoptera: Cerambycidae): Evaluation of conventional soybean resistance in the soybean plant introduction 165673, transcriptomic analyses, and gene silencing by RNA interference by Lina Maria Aguirre Rojas B. Sc., Universidad del Valle, Colombia, 2010 M. Sc., Kansas State University, 2013 AN ABSTRACT OF A DISSERTATION submitted in partial fulfillment of the requirements for the degree DOCTOR OF PHILOSOPHY Department of Entomology College of Agriculture KANSAS STATE UNIVERSITY Manhattan, Kansas 2019 ABSTRACT Dectes texanus (Coleoptera:Cerambyciade) larvae devastate soybeans in the U. S. midwestern states by girdling and tunneling inside the stems. Reduction of natural sunflower and ragweed hosts may have promoted the use soybean as a host since the late 1950s. Development of soybean varieties resistant to D. texanus is of importance to manage this pest since harvesting as soon as possible is the only option available to farmers to reduce yield losses. The soybean plant introduction (PI) 165673 reduces the number of D. texanus larvae, but survivors are still found at 21 d post infestation that may damage and girdle the PI165673 stems at the end of the growing season. Soybean resistance to D. texanus can be enhanced with the delivery of double stranded RNA (dsRNA) in planta to suppress gene expression by RNA interference (RNAi). DsRNA expressed in planta can be designed specifically to target and silence D. texanus genes important for development. D. texanus genes uniquely or highly expressed when fed soybean compared to those fed their natural hosts or artificial diet can be exploited to impair the development of D. texanus with RNAi. Although, dsRNA-based silencing is successful in other cerambycids, its use with D. texanus is untested. The objectives of this dissertation are: 1) to evaluate stem girdling and tunneling by D. texanus in soybean PI165673 at the end of the 2014 growing season in Kansas; 2) to compare the transcriptomes of D. texanus larvae fed soybean, wild sunflower, giant ragweed or artificial diet; and 3) to silence the Laccase2 (Lac2) and Chitin synthase2 (CHS2) genes in D. texanus by feeding larvae artificial diet coated with Lac2 or CHS2-dsRNA. The stems of infested PI165673 had 20% less tunneling by D. texanus larvae compared to the susceptible control K07_1544 at the end of the growing season in 2014 in Kansas. However, this result may be overestimated since the PI16563 plants had not reached full maturity and development compared to K07_1544. Therefore, assessment of stem tunneling (%) and girdling damage should be conducted in Southern States where the PI165637 can finish their development. The PI165673 resistance negatively affected the development and survival of D. texanus first instar larvae, but surviving larvae developed until the last instar stage before the end of the growing season in Kansas. These larvae can potentially gridle the PI165673 plants. A D. texanus de novo transcriptome was assembled for differential gene expression analyses. Five and six unigenes were commonly up-regulated and down-regulated in K07_1544- fed larvae compared to those fed wild sunflower, giant ragweed, or artificial diet (FDR < 0.05, fold change cut off ≥ ± 1.5). Unigenes coding for a lipocalin, an ecdysteroid kinase, and a major facilitator transporter were among the commonly up-regulated unigenes in soybean-fed larvae. Unigenes coding for two insect cuticle proteins, a glycosyl hydrolase 45, a transglutaminase, and a chitin binding peritrophin-A domain were among the commonly down-regulated unigenes in soybean fed larvae compared to either native host or artificial diet. Additionally, 41 and 13 unigenes were also up-regulated and down-regulated in larvae fed susceptible K07_1544 plants compared to those fed wild sunflower or giant ragweed, respectively. Cytochrome P450s, carboxylesterases, major facilitator transporters and glycoside hydrolases were the most represented protein families among the up-regulated unigenes in soybean-fed larvae compared to either native host. Up-regulation of unigenes involved in biotransformation of plant allelochemicals, transport of small solutes and hydrophobic molecules, and digestion of plant cell walls by larvae fed soybean may be an important factor in the ability of D. texanus to use soybean as a host. The Lac2 relative transcript level was at least 6 times lower in dsLac2-fed larvae compared to dsGFP and dsCHS2-fed larvae. However, there were no significant differences in abnormal adult morphology between dsRNA treatments. Differences in CHS2 transcript levels and larval chitin content were not detected between dsRNA treatments. Lack of treatment differences is most likely related to a small sample size in the experiment. In conclusion, the PI165673 resistance needs to be accompanied by other sources of resistance to reduce and prevent damage caused by D. texanus surviving larvae. Genes within the up-regulated protein families in larvae fed soybean could be used as potential targets for RNAi. However, further gene silencing studies are needed before soybeans expressing D. texanus targeted-dsRNA can be available. Towards the development of soybean resistance to Dectes texanus LeConte (Coleoptera: Cerambycidae): Evaluation of conventional soybean resistance in the soybean plant introduction 165673, transcriptomic analyses, and gene silencing by RNA interference by Lina Maria Aguirre Rojas B. Sc., Universidad del Valle, Colombia, 2010 M. Sc., Kansas State University, 2013 A DISSERTATION submitted in partial fulfillment of the requirements for the degree DOCTOR OF PHILOSOPHY Department of Entomology College of Agriculture KANSAS STATE UNIVERSITY Manhattan, Kansas 2019 Approved by: Major professor C. Michael Smith Copyright © Lina Maria Aguirre Rojas 2019. ABSTRACT Dectes texanus (Coleoptera:Cerambyciade) larvae devastate soybeans in the U. S. midwestern states by girdling and tunneling inside the stems. Reduction of natural sunflower and ragweed hosts may have promoted the use soybean as a host since the late 1950s. Development of soybean varieties resistant to D. texanus is of importance to manage this pest since harvesting as soon as possible is the only option available to farmers to reduce yield losses. The soybean plant introduction (PI) 165673 reduces the number of D. texanus larvae, but survivors are still found at 21 d post infestation that may damage and girdle the PI165673 stems at the end of the growing season. Soybean resistance to D. texanus can be enhanced with the delivery of double stranded RNA (dsRNA) in planta to suppress gene expression by RNA interference (RNAi). DsRNA expressed in planta can be designed specifically to target and silence D. texanus genes important for development. D. texanus genes uniquely or highly expressed when fed soybean compared to those fed their natural hosts or artificial diet can be exploited to impair the development of D. texanus with RNAi. Although, dsRNA-based silencing is successful in other cerambycids, its use with D. texanus is untested. The objectives of this dissertation are: 1) to evaluate stem girdling and tunneling by D. texanus in soybean PI165673 at the end of the 2014 growing season in Kansas; 2) to compare the transcriptomes of D. texanus larvae fed soybean, wild sunflower, giant ragweed or artificial diet; and 3) to silence the Laccase2 (Lac2) and Chitin synthase2 (CHS2) genes in D. texanus by feeding larvae artificial diet coated with Lac2 or CHS2-dsRNA. The stems of infested PI165673 had 20% less tunneling by D. texanus larvae compared to the susceptible control K07_1544 at the end of the growing season in 2014 in Kansas. However, this result may be overestimated since the PI16563 plants had not reached full maturity and development compared to K07_1544. Therefore, assessment of stem tunneling (%) and girdling damage should be conducted in Southern States where the PI165637 can finish their development. The PI165673 resistance negatively affected the development and survival of D. texanus first instar larvae, but surviving larvae developed until the last instar stage before the end of the growing season in Kansas. These larvae can potentially gridle the PI165673 plants. A D. texanus de novo transcriptome was assembled for differential gene expression analyses. Five and six unigenes were commonly up-regulated and down-regulated in K07_1544- fed larvae compared to those fed wild sunflower, giant ragweed, or artificial diet (FDR < 0.05, fold change cut off ≥ ± 1.5). Unigenes coding for a lipocalin, an ecdysteroid kinase, and a major facilitator transporter were among the commonly up-regulated unigenes in soybean-fed larvae. Unigenes coding for two insect cuticle proteins, a glycosyl hydrolase 45, a transglutaminase, and a chitin binding peritrophin-A domain were among the commonly down-regulated unigenes in soybean fed larvae compared to either native host or artificial diet. Additionally, 41 and 13 unigenes were also up-regulated and down-regulated in larvae fed susceptible K07_1544 plants compared to those fed wild sunflower or giant ragweed, respectively. Cytochrome P450s, carboxylesterases, major facilitator transporters and glycoside hydrolases were the most represented protein families among the up-regulated unigenes in soybean-fed larvae compared to either native host. Up-regulation of unigenes involved in biotransformation of plant allelochemicals, transport of small solutes and hydrophobic molecules, and digestion of plant cell
Load more

Recommended publications
  • 4Th National IPM Symposium
    contents Foreword . 2 Program Schedule . 4 National Roadmap for Integrated Pest Management (IPM) . 9 Whole Systems Thinking Applied to IPM . 12 Fourth National IPM Symposium . 14 Poster Abstracts . 30 Poster Author Index . 92 1 foreword Welcome to the Fourth National Integrated Pest Management The Second National IPM Symposium followed the theme “IPM Symposium, “Building Alliances for the Future of IPM.” As IPM Programs for the 21st Century: Food Safety and Environmental adoption continues to increase, challenges facing the IPM systems’ Stewardship.” The meeting explored the future of IPM and its role approach to pest management also expand. The IPM community in reducing environmental problems; ensuring a safe, healthy, has responded to new challenges by developing appropriate plentiful food supply; and promoting a sustainable agriculture. The technologies to meet the changing needs of IPM stakeholders. meeting was organized with poster sessions and workshops covering 22 topic areas that provided numerous opportunities for Organization of the Fourth National Integrated Pest Management participants to share ideas across disciplines, agencies, and Symposium was initiated at the annual meeting of the National affiliations. More than 600 people attended the Second National IPM Committee, ESCOP/ECOP Pest Management Strategies IPM Symposium. Based on written and oral comments, the Subcommittee held in Washington, DC, in September 2001. With symposium was a very useful, stimulating, and exciting experi- the 2000 goal for IPM adoption having passed, it was agreed that ence. it was again time for the IPM community, in its broadest sense, to come together to review IPM achievements and to discuss visions The Third National IPM Symposium shared two themes, “Putting for how IPM could meet research, extension, and stakeholder Customers First” and “Assessing IPM Program Impacts.” These needs.
    [Show full text]
  • The Genus Canidia Thomson, 1857 (Coleoptera: Cerambycidae, Lamiinae, Acanthocinini)
    Zootaxa 927: 1–27 (2005) ISSN 1175-5326 (print edition) www.mapress.com/zootaxa/ ZOOTAXA 927 Copyright © 2005 Magnolia Press ISSN 1175-5334 (online edition) The genus Canidia Thomson, 1857 (Coleoptera: Cerambycidae, Lamiinae, Acanthocinini) JAMES E. WAPPES¹ & STEVEN W. LINGAFELTER² ¹ American Coleoptera Museum, 179 Fall Creek, Bulverde, TX 78163 U. S. A. [email protected] ² Systematic Entomology Lab, Plant Sciences Institute, Agriculture Research Service, USDA, National Museum of Natural History, Smithsonian Institution, MRC-168, Washington, DC 20013-7012 U. S. A. [email protected] Abstract The lamiine genus Canidia Thomson is redefined with Canidiopsis Dillon and Pseudocanidia Dil- lon as new synonyms. Three new species from Mexico are described and illustrated: Canidia chemsaki, C. giesberti, and C. turnbowi. The following new synonymies are proposed: Canidiop- sis similis Dillon, 1955 and Canidiopsis hebes Dillon, 1955 = Canidia mexicana Thomson, 1860; Pseudocanidia cuernavacae Dillon, 1955 = Dectes spinicornis Bates, 1881; and Dectes (Canidia) balteata var. inapicalis Tippmann, 1960 = Dectes balteatus Lacordaire, 1872. A key to the eight species and one subspecies is presented. Key words: Insecta, Coleoptera, Cerambycidae, Lamiinae, Acanthocinini, Canidia, Dectes, Can- idiopsis, Pseudocanidia, new species, key Resumen: Se redefine el género Canidia Thomson con Canidiopsis Dillon y Pseudocanidia Dillon como sinónimos nuevos. Describimos e ilustramos tres especies nuevas de México: Canidia chemsaki, C. giesberti y C. turnbowi. Se proponen los siguientes sinónimos nuevos: Canidiopsis similis Dillon, 1955 y Canidiopsis hebes Dillon 1955 = Canidia mexicana Thomson, 1860; Pseudocanidia cuernavacae Dillon, 1955 = Dectes spinicornis Bates, 1881; y Dectes (Canidia) balteata inapicalis Tippmann, 1960 = Dectes balteatus Lacordaire, 1872. Se incluye una clave para separar las ocho especies y una subespecie.
    [Show full text]
  • 4 Reproductive Biology of Cerambycids
    4 Reproductive Biology of Cerambycids Lawrence M. Hanks University of Illinois at Urbana-Champaign Urbana, Illinois Qiao Wang Massey University Palmerston North, New Zealand CONTENTS 4.1 Introduction .................................................................................................................................. 133 4.2 Phenology of Adults ..................................................................................................................... 134 4.3 Diet of Adults ............................................................................................................................... 138 4.4 Location of Host Plants and Mates .............................................................................................. 138 4.5 Recognition of Mates ................................................................................................................... 140 4.6 Copulation .................................................................................................................................... 141 4.7 Larval Host Plants, Oviposition Behavior, and Larval Development .......................................... 142 4.8 Mating Strategy ............................................................................................................................ 144 4.9 Conclusion .................................................................................................................................... 148 Acknowledgments .................................................................................................................................
    [Show full text]
  • Program Book
    NORTH CENTRAL BRANCH Entomological Society of America 59th Annual Meeting March 28-31, 2004 President Rob Wiedenmann The Fairmont Kansas City At the Plaza 401 Ward Parkway Kansas City, MO 64112 Contents Meeting Logistics ................................................................ 2 2003-2004 Officers and Committees, ESA-NCB .............. 4 2004 North Central Branch Award Recipients ................ 8 Program ............................................................................. 13 Sunday, March 28, 2004 Afternoon ...............................................................13 Evening ..................................................................13 Monday, March 29, 2004 Morning..................................................................14 Afternoon ...............................................................23 Evening ..................................................................42 Tuesday, March 30, 2004 Morning..................................................................43 Afternoon ...............................................................63 Evening ..................................................................67 Wednesday, March 31, 2004 Morning..................................................................68 Afternoon ...............................................................72 Author Index ..............................................................73 Taxonomic Index........................................................84 Key Word Index.........................................................88
    [Show full text]
  • BIOLOGY and MANAGEMENT of the SOYBEAN STEM BORER, Dectes Texanus Leconte, in KENTUCKY
    University of Kentucky UKnowledge Theses and Dissertations--Entomology Entomology 2019 BIOLOGY AND MANAGEMENT OF THE SOYBEAN STEM BORER, Dectes texanus LeConte, IN KENTUCKY Izabela Gomes University of Kentucky, [email protected] Digital Object Identifier: https://doi.org/10.13023/etd.2019.448 Right click to open a feedback form in a new tab to let us know how this document benefits ou.y Recommended Citation Gomes, Izabela, "BIOLOGY AND MANAGEMENT OF THE SOYBEAN STEM BORER, Dectes texanus LeConte, IN KENTUCKY" (2019). Theses and Dissertations--Entomology. 49. https://uknowledge.uky.edu/entomology_etds/49 This Master's Thesis is brought to you for free and open access by the Entomology at UKnowledge. It has been accepted for inclusion in Theses and Dissertations--Entomology by an authorized administrator of UKnowledge. For more information, please contact [email protected]. STUDENT AGREEMENT: I represent that my thesis or dissertation and abstract are my original work. Proper attribution has been given to all outside sources. I understand that I am solely responsible for obtaining any needed copyright permissions. I have obtained needed written permission statement(s) from the owner(s) of each third-party copyrighted matter to be included in my work, allowing electronic distribution (if such use is not permitted by the fair use doctrine) which will be submitted to UKnowledge as Additional File. I hereby grant to The University of Kentucky and its agents the irrevocable, non-exclusive, and royalty-free license to archive and make accessible my work in whole or in part in all forms of media, now or hereafter known.
    [Show full text]
  • Open Thesis Maya E. Nehme.Pdf
    The Pennsylvania State University The Graduate School Department of Entomology DEVELOPING MONITORING TRAPS FOR THE ASIAN LONGHORNED BEETLE A Dissertation in Entomology & Comparative and International Education by Maya E. Nehme © 2009 Maya E. Nehme Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy August 2009 The dissertation of Maya E. Nehme was reviewed and approved* by the following: Kelli Hoover Associate Professor of Entomology Dissertation Advisor Co-Chair of Committee Edwin Rajotte Professor of Entomology, IPM Coordinator and CI ED joint faculty Co-Chair of Committee Thomas Baker Professor of Entomology Melody Keena US Forest Service Research Entomologist and Adjunct Faculty of Entomology Special member David Baker Professor of Education, Professor of Sociology Gary Felton Professor of Entomology Head of the Department of Entomology *Signatures are on file in the Graduate School ii ABSTRACT Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae: Lamiinae), commonly known as the Asian longhorned beetle, is a wood-boring invasive species introduced from Asia to North America and Europe through solid wood packing material. A. glabripennis is a serious pest both in China and the U.S. This research project was developed in response to the need for efficient monitoring traps to assess population density and dispersal in the field and to detect new introductions at ports of entry. The first stages of the project aimed at filling the gaps in our knowledge of the effect of semiochemicals on A. glabripennis adult behavior and exploring potential use of these chemicals for monitoring purposes. Semiochemicals studied were the male- produced putative volatile pheromone vbutan-1-ol and 4-(n-heptyloxy)butanal) and plant volatiles.
    [Show full text]
  • Inheritance of Antibiosis Resistance to the Dectes Stem Borer, Dectes Texanus, in Soybean PI165673
    agronomy Article Inheritance of Antibiosis Resistance to the Dectes Stem Borer, Dectes texanus, in Soybean PI165673 Lina M. Aguirre-Rojas 1, Lawrent L. Buschman 2, Brian McCornack 2, William T. Schapaugh 3, Erin D. Scully 4, Kun Yan Zhu 2 , Harold N. Trick 5 and Charles Michael Smith 2,* 1 Department of Botany and Plant Sciences, University of California Riverside, Riverside, CA 92506, USA; [email protected] 2 Department of Entomology, Kansas State University, Manhattan, KS 66506, USA; [email protected] (L.L.B.); [email protected] (B.M.); [email protected] (K.Y.Z.) 3 Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA; [email protected] 4 Stored Product Insect and Engineering Research Unit, USDA-ARS-CGAHR, Manhattan, KS 66502, USA; [email protected] 5 Department of Plant Pathology, Kansas State University, Manhattan, KS 66502, USA; [email protected] * Correspondence: [email protected] Abstract: Dectes texanus larvae girdles the stems of soybean and cultivated sunflowers causing significant yield losses in North America. The soybean Plant Introduction (PI) 165673 exhibits antibiosis resistance to the larval stage. The objectives of this study were: (1) to determine the inheritance of D. texanus resistance in PI165673; (2) evaluate PI165673 antibiosis resistance before 21 d post infestation; (3) evaluate girdling damage in PI16563 at the end of the season. K07-1544/PI165673 F2 and F2:3 populations were tested for resistance to D. texanus in 2011 and 2012, and PI165673 antibiosis resistance and girdling damage were evaluated in 2014. Segregation for resistance to D. texanus and heritability estimates in the F2 and F2:3 populations indicated that resistance was Citation: Aguirre-Rojas, L.M.; controlled by two genes with dominant and recessive epistasis.
    [Show full text]
  • Samano Et Al 2012 Monochamus Pheromones CE
    821 2-Undecyloxy-1-ethanol in combination with other semiochemicals attracts three Monochamus species (Coleoptera: Cerambycidae) in British Columbia, Canada Jorge E. Macias-Samano,1 David Wakarchuk, Jocelyn G. Millar, Lawrence M. Hanks Abstract—Two species in the genus Monochamus Dejean (Coleoptera: Cerambycidae: Lamiinae) have recently been shown to have the same male-produced sex pheromone, 2-undecyloxy-1-ethanol (monochamol), suggesting that other congeners may share the same pheromone. We tested that hypothesis by conducting field bioassays of monochamol, in combination with bark-beetle pheromones and the host plant volatiles ethanol and a-pinene, in southern British Columbia, Canada. We captured 603 Monochamus clamator (LeConte), 63 Monochamus obtusus Casey, 245 Monochamus scutellatus (Say) (tribe Monochamini), and 42 Acanthocinus princeps (Walker) (tribe Acanthocinini). All three Mono- chamus species were significantly attracted to the combination of monochamol and host plant volatiles, whereas bark-beetle pheromones plus plant volatiles and plant volatiles alone were minimally attractive. Adding bark-beetle pheromones to the monochamol plus plant volatiles treatment synergised attraction of M. clamator, but not the other two Monochamus species. Acanthocinus princeps was most strongly attracted to the combination of bark-beetle pheromones and plant volatiles, and did not appear to be affected by the presence or absence of monochamol in baits. We conclude that monochamol is a likely pheromone component for the three Monochamus species, and that monochamol plus host plant volatiles is an effective attractant for these and perhaps other North American Monochamus species. Re´sume´—On a de´montre´re´cemment que deux espe`ces du genre Monochamus Dejean (Coleoptera: Cerambycidae: Lamiinae) posse´daient la meˆme phe´romone sexuelle maˆle, le 2-unde´cyloxy-1-e´thanol (monochamol), ce qui laisse croire que d’autres conge´ne`res pourraient aussi avoir cette meˆme phe´romone.
    [Show full text]
  • The Phylogeny and Evolutionary Biology of the Pimplinae (Hymenoptera : Ichneumonidae)
    THE PHYLOGENY AND EVOLUTIONARY BIOLOGY OF THE PIMPLINAE (HYMENOPTERA : ICHNEUMONIDAE) Paul Eggleton A thesis submitted for the degree of Doctor of Philosophy of the University of London Department of Entomology Department of Pure & Applied B ritish Museum (Natural H istory) Biology, Imperial College London London May 1989 ABSTRACT £ The phylogeny and evolutionary biology of the Pimplinae are investigated using a cladistic compatibility method. Cladistic methodology is reviewed in the introduction, and the advantages of using a compatibility method explained. Unweighted and weighted compatibility techniques are outlined. The presently accepted classification of the Pimplinae is investigated by reference to the diagnostic characters used by earlier workers. The Pimplinae do not form a natural grouping using this character set. An additional 22 new characters are added to the data set for a further analysis. The results show that the Pimplinae (sensu lato) form four separate and unconnected lineages. It is recommended that the lineages each be given subfamily status. Other taxonomic changes at tribal level are suggested. The host and host microhabitat relations of the Pimplinae (sensu s tr ic to ) are placed within the evolutionary framework of the analyses of morphological characters. The importance of a primitive association with hosts in decaying wood is stressed, and the various evolutionary pathways away from this microhabitat discussed. The biology of the Rhyssinae is reviewed, especially with respect to mating behaviour and male reproductive strategies. The Rhyssinae (78 species) are analysed cladistically using 62 characters, but excluding characters thought to be connected with mating behaviour. Morphometric studies show that certain male gastral characters are associated with particular mating systems.
    [Show full text]
  • ESA 2 0 14 9-12 March 2014 Des Moines, Iowa 2014 NCB-ESA Corporate Sponsors CONTENTS
    NCB ESA 2 0 14 9-12 March 2014 Des Moines, Iowa 2014 NCB-ESA Corporate Sponsors CONTENTS Meeting Logistics ....................................................1 2014 NCB-ESA Officers and Committees .................5 2014 Award Recipients ...........................................7 Sunday, 9 March 2014 At-a-Glance ..................................................18 Afternoon .....................................................19 Monday, 10 March 2014 At-a-Glance ..................................................23 Posters .........................................................25 Morning .......................................................30 Afternoon .....................................................35 Tuesday, 11 March 2014 At-a-Glance ..................................................45 Posters .........................................................47 Morning .......................................................51 Afternoon .....................................................55 Wednesday, 12 March 2014 At-a-Glance ..................................................60 Morning .......................................................61 Author Index ........................................................67 Scientific Name Index ...........................................77 Keyword Index ......................................................82 Common Name Index ...........................................83 Map of Meeting Facilities ..............inside back cover i MEETING LOGISTICS Registration All participants must register
    [Show full text]
  • Beiträge Zur Bayerischen Entomofaunistik 13: 67–207
    Beiträge zur bayerischen Entomofaunistik 13:67–207, Bamberg (2014), ISSN 1430-015X Grundlegende Untersuchungen zur vielfältigen Insektenfauna im Tiergarten Nürnberg unter besonderer Betonung der Hymenoptera Auswertung von Malaisefallenfängen in den Jahren 1989 und 1990 von Klaus von der Dunk & Manfred Kraus Inhaltsverzeichnis 1. Einleitung 68 2. Untersuchungsgebiet 68 3. Methodik 69 3.1. Planung 69 3.2. Malaisefallen (MF) im Tiergarten 1989, mit Gelbschalen (GS) und Handfänge 69 3.3. Beschreibung der Fallenstandorte 70 3.4. Malaisefallen, Gelbschalen und Handfänge 1990 71 4. Darstellung der Untersuchungsergebnisse 71 4.1. Die Tabellen 71 4.2. Umfang der Untersuchungen 73 4.3. Grenzen der Interpretation von Fallenfängen 73 5. Untersuchungsergebnisse 74 5.1. Hymenoptera 74 5.1.1. Hymenoptera – Symphyta (Blattwespen) 74 5.1.1.1. Tabelle Symphyta 74 5.1.1.2. Tabellen Leerungstermine der Malaisefallen und Gelbschalen und Blattwespenanzahl 78 5.1.1.3. Symphyta 79 5.1.2. Hymenoptera – Terebrantia 87 5.1.2.1. Tabelle Terebrantia 87 5.1.2.2. Tabelle Ichneumonidae (det. R. Bauer) mit Ergänzungen 91 5.1.2.3. Terebrantia: Evanoidea bis Chalcididae – Ichneumonidae – Braconidae 100 5.1.2.4. Bauer, R.: Ichneumoniden aus den Fängen in Malaisefallen von Dr. M. Kraus im Tiergarten Nürnberg in den Jahren 1989 und 1990 111 5.1.3. Hymenoptera – Apocrita – Aculeata 117 5.1.3.1. Tabellen: Apidae, Formicidae, Chrysididae, Pompilidae, Vespidae, Sphecidae, Mutillidae, Sapygidae, Tiphiidae 117 5.1.3.2. Apidae, Formicidae, Chrysididae, Pompilidae, Vespidae, Sphecidae, Mutillidae, Sapygidae, Tiphiidae 122 5.1.4. Coleoptera 131 5.1.4.1. Tabelle Coleoptera 131 5.1.4.2.
    [Show full text]
  • Cerambycidae of Tennessee
    Cerambycidae of Tennessee! Disteniinae: Disteniini! Parandrinae: Parandriini! Closed circles represent previously published county records, museum specimen records, and specimens examined. Open circles are county records reported in Jamerson (1973) for which a specimen could not be located. Future collections are needed to substantiate these accounts. Fig. 2. Elytrimitatrix (Elytrimitatrix) undata (F.)! Fig. 3. Neandra brunnea (F.)! Prioninae: Macrotomini! Prioninae: Meroscheliscini! Fig. 4. Archodontes melanoplus melanoplus (L.)! Fig. 5. Mallodon dasystomus dasystomus Say! Fig. 6. Tragosoma harrisii (LeConte)! Prioninae: Prionini! Fig. 7. Derobrachus brevicollis Audinet-Serville! Fig. 8. Orthosoma brunneum (Forster)! Fig. 9. Prionus (Neopolyarthron) imbricornis (L.)! Prioninae! : Solenopterini! Fig. 10. Prionus (Prionus) laticollis (Drury) ! Fig. 11. Prionus (Prionus) pocularis Dalman ! Fig. 12. Sphenosethus taslei (Buquet) ! Necydalinae: Necydalini! Spondylidinae: Asemini! Fig. 13. Necydalis melitta (Say)! Fig. 14. Arhopalus foveicollis (Haldeman)! Fig. 15. Arhopalus rusticus obsoletus (Randall)! ! ! Suppl. Figs. 2-15. Tennessee county collection localities for longhorned beetle (Cerambycidae) species: Disteniinae, Parandrinae, Prioninae, Necydalinae, Spondylinae: Asemini (in part). ! Spondylidinae: Asemini (ctd.)! Fig. 16. Asemum striatum (L.)! Fig. 17. Tetropium schwarzianum Casey! Fig. 18. Atimia confusa confusa (Say)! ! Spondylidinae: Saphanini! Lepturinae: Desmocerini! Lepturinae: Encyclopini! Fig. 19. Michthisoma heterodoxum LeConte
    [Show full text]