PLOS ONE RESEARCH ARTICLE Behavioural and cognitive changes in aged pet dogs: No effects of an enriched diet and lifelong training 1,2 3,4 1,2 5 Durga ChapagainID *, Lisa J. Wallis , Friederike Range , Nadja Affenzeller , Jessica Serra6, Zso fia ViraÂnyi1 1 Clever Dog Lab, Comparative Cognition, Messerli Research Institute, University of Veterinary Medicine Vienna, Medical University of Vienna, University of Vienna, Vienna, Austria, 2 Domestication Lab, Konrad Lorenz Institute of Ethology, University of Veterinary Medicine Vienna, Vienna, Austria, 3 Department of a1111111111 Livestock and One Health, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, a1111111111 Liverpool, United Kingdom, 4 Department of Ethology, EoÈtvoÈs LoraÂnd University, Budapest, Hungary, a1111111111 5 Department/Clinic for Companion Animals and Horses, University of Veterinary Medicine Vienna, Vienna, a1111111111 Austria, 6 Royal Canin Research Centre, Aimargues, France a1111111111 *
[email protected] Abstract OPEN ACCESS Dogs demonstrate behavioural changes and cognitive decline during aging. Compared to Citation: Chapagain D, Wallis LJ, Range F, Affenzeller N, Serra J, ViraÂnyi Z (2020) Behavioural laboratory dogs, little is known about aging in pet dogs exposed to different environments and cognitive changes in aged pet dogs: No effects and nutrition. In this study, we examined the effects of age, an enriched diet and lifelong of an enriched diet and lifelong training. PLoS ONE training on different behavioural and cognitive measures in 119 pet dogs (>6yrs). Dogs were 15(9): e0238517. https://doi.org/10.1371/journal. pone.0238517 maintained on either an enriched diet or a control diet for one year. Lifelong training was cal- culated using a questionnaire where owners filled in their dog's training experiences to date.