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Biography: ALN Rao, UC-Riverside Biography: A.L.N. Rao, UC-Riverside BIOGRAPHY A. Professional Preparation Name: A. L.N. Rao Institution Major Degree Year A.P. Agricultural University, India Agriculture B.Sc(Ag) 1972 Indian Agricultural Research Institute Plant Pathology M.Sc 1976 University of Adelaide, South Australia Plant Pathology Ph.D 1982 B. Appointments 2004- present Professor, Dept. Plant Pathology & Microbiology, University of California, Riverside, 1997-2004 Associate Professor, Dept. Plant Pathology & Microbiology, University of California, Riverside, 1993-1997 Assistant Professor, Dept. of Plant Pathology, University of California, Riverside, CA. 1986-1993 Research Associate, Texas A & M University, College Station, Texas. 1982-1986 Postdoctoral Fellow, University of Alberta, Edmonton, Alberta, Canada. C. Research Interests A fundamental question in virology is how viruses package their genomes into stable virions. The major focus of research in my laboratory is to understand the replication and mechanism of genome packaging in RNA viruses of plants and insects with divided genomes. To this end, we are using Brome mosaic virus (BMV), a tripartite plant infecting RNA virus as a model system for analyzing various molecular interactions between coat protein and RNA leading to assembly of mature infectious virions. In addition, we are also applying molecular and proteomics approaches for dissecting the mechanism regulating the nuclear localization and replication of a satellite RNA of Cucumber mosaic virus and replication of Satellite tobacco mosaic virus (STMV). More recently, we initiated a projected to initiate the assembly of virus like particles of Human Immuno deficiency Virus (HIV) and Ebola Virus (EBV) in plants with an eventual aim of developing novel strategies of vaccine development. D. Synergistic activities 2015- Member, NSF, GRFP Grant Review Panel 2013- Editorial Board, Virology 2013- Academic Editor, PLoS ONE 2006-2009 Senior Editor (Virology), Phytopathology 2006 Ad Hoc reviewer, NIH Study Section, Membrane Biology & Protein Processing 2002 Chairman and Organizer Plant Virology Satellite Symposium at ASV 2003 Chairman and Organizer Plant Virology Satellite Symposium at ASV 1 Biography: A.L.N. Rao, UC-Riverside 2004 Chairman and Organizer Plant Virology Satellite Symposium at ASV 2005 Chairman and Organizer Plant Virology Satellite Symposium at ASV E. Journal articles (JA) (Referred) 1. Sonali Chaturvedi and A. L. N. Rao (2017). Molecular and biological factors regulating the genome packaging in single-strand positive-sense tripartite RNA plant viruses. Current Opinion in Virology (In Press). 2. Sonali Chaturvedi and A. L. N. Rao (2017). Riboproteomics: A versatile approach for the identification of host protein interaction network in plant pathogenic noncoding RNAs. PLoS ONE 12(10) https://doi.org/10.1371/journal.pone.0186703. 3. Venkatesh Sivanandam, Deborah Mathews, Rees Garmann, Gonca Erdemci- Tandogan, Roya Zandi and A. L. N. Rao (2016). Functional analysis of the N- terminal basic motif of a eukaryotic satellite RNA virus capsid protein in replication and packaging. Scientific Reports. 6, 26328. 4. Sonali Chaturvedi., Jang-Kyun Seo and A. L. N. Rao (2016). Functionality of host proteins in Cucumber mosaic virus replication: GAPDH is obligatory to promote interaction between replication-associated proteins. Virology 494: 47-55. 5. Sonali Chaturvedi and A. L. N. Rao (2016). A shift in plant proteome profile for a Bromodomain containing RNA binding Protein (BRP1) in plants infected with cucumber mosaic virus and its satellite RNA. Journal of Proteomics 131: 1-7. 6. Sivanandam, V., Mathews, D and A.L.N. Rao (2015) Properties of Satellite tobacco mosaic virus phenotypes expressed in the presence and absence of helper virus. Virology 483: 163-173. 7. Venkatesh Sivanandam, Erika Varady and A. L. N. Rao (2015) Heterologous replicase driven 3' end repair of Cucumber mosaic virus satellite RNA. Virology: 478: 18-26. 8. Dong Gui, Sharad Gupta, Jun Xu, Roya Zandi, Sarjeet Gill, I-Chueh Huang, A.L.N. Rao and Umar Mohideen (2014). A novel minimal in vitro system for analyzing HIV-1 Gag mediated budding. J. Biological Physics. 41(2) 135-149. 9. Gopal, A.J., Egecioglu, D., Yoffe, A, Ben-Shaul. A., Rao, A., Knobler, C., and Gelbart, W (2014). Viral RNAs are unusually Compact. PLOS ONE 9 (9) e105875. 10. Sonali Chaturvedi and A.L.N. Rao (2014) Live Cell Imaging of Interactions Between Replicase and Capsid Protein of Brome Mosaic Virus using Bimolecular Fluorescence Complementation: Implications for Replication and Genome Packaging. Virology 464-465: 67-75. 11. Sonali Chaturvedi, Kriton Kalantidis and A. L. N. Rao (2014). A bromodomain containing host protein mediates the nuclear import of a satellite RNA of cucumber mosaic virus. Journal of Virology 88(4) 1890-1896. 2 Biography: A.L.N. Rao, UC-Riverside 12. Sun-Jung Kwon, Sonali Chaturvedi and A. L. N. Rao (2014). Repair of the 3' proximal and internal deletions of a satellite RNA associated with Cucumber mosaic virus is directed toward restoring structural integrity. Virology 450-451: 222-232. 13. Bamunusinghe, D., Sonali Chaturvedi, Jang-Kyun Seo and A. L. N. Rao (2013). Mutations in the Capsid Protein of Brome Mosaic Virus Affecting Encapsidation Eliminate Vesicle Induction in planta: Implications for Virus Cell-to-Cell Spread. Journal of Virology 87:8982-892. 14. Sharad Gupta, Muhammad R. Chatni , Ayala L. N. Rao , Valentine I. Vullev , Lihong V. Wang and Bahman Anvari (2013). Virus-mimicking nano- constructs as a contrast agent for near infrared photoacoustic imaging. Nanoscale 5: 1772-1776. 15. Jang-Kyun Seo, Sun-Jung Kwon, Sonali Chaturvedi and A.L.N. Rao (2013). Functional significance of a hepta nucleotide motif present at the junction of cucumber mosaic virus satellite RNA multimers in helper-virus-dependent replication. Virology 435: 214-219. 16. Mauricio Comas-Garcia, Ruben D. Cadena-Nava, A. L. N. Rao, Charles M. Knobler, and William M. Gelbart (2012) In Vitro Quantification of the Relative Packaging Efficiencies of Single-Stranded RNA Molecules by Viral Capsid Protein. J. Virol. 86: 12271-12282. 17. Jang-Kyun Seo, Sun-Jung Kwon and A.L.N. Rao (2012). Molecular dissection of Flock house virus protein B2 reveals that electrostatic interactions between N- terminal domains of B2 monomers are critical for dimerization. Virology 432: 296- 305. 18. Jang-Kyun Seo, Sun-Jung Kwon and A.L.N. Rao (2012). A physical interaction between viral replicase and capsid protein is required for genome packaging specificity in an RNA virus. Journal of Virology 86(11): 6210-6221. 19. Soon Ho Choi, Jang-Kyun Seo, Sun-Jung Kwon and A. L. N. Rao (2012). Helper virus independent transcription and multimerization of a satellite RNA associated with cucumber mosaic virus. Journal of Virology 86(9): 4823-4832. 20. Sun-Jung Kwon and A. L.N. Rao (2012). Emergence of distinct brome mosaic virus recombinants is determined by the polarity of the inoculum RNA. Journal of Virology 86(9): 5204-5220. 21. Cadena-Nava, R., Mauricio Comas-Garcia., Rees Garmann., A.L.N. Rao., Charles Knobler and William Gelbart (2012). Self-assembly of viral capsid protein and RNA molecules of different sizes: requirement for a specific high protein/RNA mass ratio. Journal of Virology 86(6): 3318-3326. 22. Chaturvedi, S., S. Gupta, B. Jung., B. Anvari and A. L. N. Rao (2012). A simple and robust in vivo and in vitro approach for studying assembly in RNA viruses. Journal of Visualization Experiment. March 2012, vol 61:e3645. 23. Jung, B., A. L. N. Rao and Bahman Anvari (2011). Optical nano constructs composed of genome depleted brome mosaic virus doped with a near infrared chromophore for potential biomedical applications. ACS Nano 5(2) 1243-1252. (Featured as cover article). 3 Biography: A.L.N. Rao, UC-Riverside 24. Bamunusinghe, D., Jang-Kyun Seo and A. L. N. Rao (2011). Subcellular localization and rearrangement of endoplasmic reticulum by brome mosaic virus capid protein. Journal of Virology 85(6) 2953-2963. 25. de Wispelaere, M and A. L. N. Rao. (2011). Packaging and structural phenotype of brome mosaic virus capsid protein with altered N-terminal β-hexamer structure. Virology 419: 17-23. 26. de Wispelaere, M and A. L. N. Rao. (2009). Production of cucumber mosaic virus RNA5 and its role in recombination. Virology 384: 179-191. 27. Annamalai, P. Fady Rofail, D.A. Demason and A.L.N. Rao, 2008. Replication coupled packaging mechanism in positive-strand RNA viruses; Synchronized co- expression of functional multigenome components of an animal and plant virus in Nicotiana benthamiana cells by agroinfiltration. Journal of Virology 82: 1484- 1495. This paper was selected as “Articles of Significant Interest (Spot light)” by the Journal of Virology Editorial Board. 28. Calhoun, S.L. and A. L.N. Rao. 2008. Functional analysis of brome mosaic virus coat protein-RNA interacting domains. Archives of Virology 153: 231-245. 29. Annamalai Padmanaban and A.L.N. Rao. 2007. In vivo packaging of brome mosaic virus RNA3, but not RNAs 1 and 2, is dependent on a cis-acting 3’ tRNA- like structure. Journal of Virology 81: 173-181. 30. Calhoun, S.L., Speir, J.A., A. L. N. Rao. 2007. In vivo polymorphism results from deletion of a N-terminal peptide molecular switch in brome mosaic virus capsid protein. Virology 364: 407-421. 31. Annamalai Padmanaban and A.L.N. Rao. 2006. Packaging of brome mosaic virus subgenomic RNA is functionally coupled to replication-dependent transcription and translation of coat protein. Journal of Virology 80:10096-10108. This paper was selected as “Articles of Significant Interest (Spot light)” by the Journal of Virology Editorial Board. 32. Rao, A.L.N. 2006. Sensitivity of brome mosaic virus replication to 3’ mutations in RNA1 implies a requirement for sustained synthesis of 1a replicase protein. Arch. Virol. 151: 721-733. 33. Rao, A.L.N. and Bret Cooper. 2006. Capsid protein gene and the type of host plant differentially modulate cell-to-cell movement of cowpea chlorotic mottle virus.
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