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AVALIAÇÃO DE ATIVIDADES BIOLÓGICAS DA Turnera Subulata

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AVALIAÇÃO DE ATIVIDADES BIOLÓGICAS DA Turnera Subulata UNIVERSIDADE FEDERAL DE PERNAMBUCO CENTRO DE CIÊNCIAS BIOLÓGICAS MESTRADO EM BIOQUÍMICA E FISIOLOGIA AVALIAÇÃO DE ATIVIDADES BIOLÓGICAS DA Turnera subulata ALUNA: THASSIANY REBECA PAIVA MOURA DA SILVA ORIENTADORA: PROFª. DRª. MARIA TERESA JANSEM DE A. CATANHO CO-ORIENTADORA: PROFª. DRª SIMEY DE SOUZA LEÃO PEREIRA MAGNATA RECIFE, 2012 Avaliação de Atividades Biológicas da Turnera subulata SILVA, T.R.P.M. THASSIANY REBECA PAIVA MOURA DA SILVA AVALIAÇÃO DE ATIVIDADES BIOLÓGICAS DA TURNERA SUBULATA Dissertação apresentada para o cumprimento parcial das exigências para obtenção do Título de Mestre em Bioquímica e Fisiologia pela Universidade Federal de Pernambuco. RECIFE, 2012 2 Avaliação de Atividades Biológicas da Turnera subulata SILVA, T.R.P.M. Elaine Barroso CRB 1728 Silva, Thassiany Rebeca Paiva Moura da Avaliação de atividades biológicas da Turnera subulata / Thassiany Rebeca Paiva Moura da Silva. – Recife: O Autor, 2012. 51 folhas : il., fig., tab. Orientadora: Maria Teresa Jansem de A. Catanho Coorientadora: Simey de Souza Leão Pereira Magnata Dissertação (mestrado) – Universidade Federal de Pernambuco, Centro de Ciências Biológicas. Ciências Biológicas, 2012. Inclui bibliografia e anexos 1. Turneraceae 2. Matéria médica vegetal 3. Cicatrização de feridas I. Catanho, Maria Teresa Jansem de A. (orientadora) II. Magnata, Simey de Souza Leão Pereira (coorientadora) III. Título 583.626 CDD (22.ed.) UFPE/CCB-2012-208 3 Avaliação de Atividades Biológicas da Turnera subulata SILVA, T.R.P.M. ÍNDICE ANALÍTICO AGRADECIMENTOS ..................................................................................................................... 04 LISTA DE FIGURAS....................................................................................................................... 05 LISTA DE TABELAS....................................................................................................................... 06 RESUMO....................................................................................................................... ..................... 07 ABSTRACT......................................................................... ............................................................... 08 1.INTRODUÇÃO.............................................................................................................................. 09 1.1 TURNERACEAE. ............................. ............................................................................................ 10 1.2 TURNERA SUBULATA ................................................................................................................. 11 1.3 CICATRIZAÇÃO ..... .................................................................................................................... 13 1.4 FITOQUÍMICA ........................................................................................................................ .... 14 1.5 TOXICIDADE AGUDA ............................................................................................................... 15 1.6 FRAGILIDADE OSMÓTICA ....................................................................................... ............... 16 2.REFERÊNCIAS............................................................................................................................. 18 3.OBJETIVOS................................................................................................................................... 22 3.1GERAL 3.2 ESPECÍFICOS 4.ARTIGO......................................................................................................................................... 23 AVALIAÇÃO DE ATIVIDADES BIOLÓGICAS DA TURNERA SUBULATA RESUMO........................................................................................................................................... 24 ABSTRACT........................................................................................................................................ 25 4.1 INTRODUÇÃO............................................................................................................................ 26 5. MATERIAIS E MÉTODOS 5.1 MATERIAL BOTÂNICO ............................................................................................................. 28 5.2 PREPARO DO EXTRATO AQUOSO DE T. SUBULATA .......................................................... 28 5.3 ANÁLISE FITOQUÍMICA .... ....................................................................................................... 28 5.4 ANIMAIS EXPERIMENTAIS ..................................................................................................... 28 5.5 TOXICIDADE AG UDA .............................................................................................................. 29 5.6 PRODUÇÃO DO GEL. ................................................................................................................ 29 5.7 INDUÇÃO DE FERIDA ABERTA EM CAMUNDONGOS ...................................................... 29 5.8 TRATAMENTO DAS FERIDAS ABERTAS. ............................................................................ 30 5.9 AVALIAÇÃO MACROSCÓPICA DA FERIDA ... ..................................................................... 30 5.10 FRAGILIDADE OSMÓTICA .................................................................................................... 30 5.11 AVALIAÇÃO METABOLISMO BIOQUÍMICO ............. .......... ........................... ................. 31 5.12 ANÁLISE ESTATÍSTICA .......................................................................................................... 32 6. RESULTADOS 6.1 FITOQUÍMICA ............................... ............................................................................................. 32 6.2 TOXICIDADE AGUDA .............................................................................................................. 33 6.3 INDUÇÃO DE FERIDA A BERTA/ CICATRIZAÇÃO ............................................................. 33 6.4 FRAGILIDADE OSMÓTICA ...................................................................................................... 34 6.5 AVALIAÇÃO DAS ALTERAÇÕES DO METABOLI SMO BIOQUÍMICO .......... ................... 35 7.CONCLUSÕES.................................................................................................................. ............. 41 8. REFERÊNCIAS BIBLIOGRÁFICAS................................... ...................................................... 42 9.ANEXOS 9.1 SISTEMAS D E ANÁLISES FITOQUÍMICAS .. .. ....................................................................... 49 9.2 ACOMPANHAMENTO MACROSCÓPICO DO PROCESSO DE CICATRIZAÇÃO ................................................................................................................................ 49 9.3 ATIVIDADES PARALELAS ....................................................................................................... 51 4 Avaliação de Atividades Biológicas da Turnera subulata SILVA, T.R.P.M. AGRADECIMENTOS À Deus , o único, fortaleza minha, o meu rochedo, o meu lugar forte, em quem confio, o meu escudo, a força da minha salvação e o meu alto refúgio, meu pastor e guia, por todo seu amor incondicional. “Porque os meus pensamentos não são os vossos pensamentos, nem os vossos caminhos, os meus caminhos, diz o Senhor. Porque, assim como os céus são mais altos do que a terra, assim são os meus caminhos mais altos do que os vossos caminhos, e os meus pensamentos, mais altos do que os vossos pensamentos.” Isaías 55. 8,9 Aos meus Pais e Irmãos por todo amor, apoio, compreensão e renúncia. Vocês são muito especiais. “O filho sábio alegrará a seu pai, mas o homem insensato despreza a sua mãe.” Provérbios 15.20 Aos professores e Amigos por todo apoio, incentivo e paciência. “O Homem que tem muitos amigos pode congratular-se, mas há amigo mais chegado do que um irmão.” Provérbios 18. 24 E por fim a todos... “ O meu Deus, segundo as suas riquezas, suprirá todas as vossas necessidades em glória, por Cristo Jesus.” Filipenses 4.19 “Feliz o homem cuja força está em ti, em cujo coração estão os caminhos aplanados.” Salmos 84 .5 Cada dia aqui e eternamente... Muito Grata por tudo! 5 Avaliação de Atividades Biológicas da Turnera subulata SILVA, T.R.P.M. LISTA DE FIGURAS Figura 1: Foto de Arbustos de Turnera subulata : evidenciando as folhas e a flor.............12 Figura 2: Distribuição geográfica no Brasil da espécie Turnera subulata ..........................12 Figura 3: Gráfico da Fragilidade osmótica de amostras de sangue tratados e não tratados com extrato de T. subulata ....................................................................................................35 Figura 4 : Gráfico da cinética da glicose nos tempos de 0,2, 4, 8, 12 e 24horas..................36 Figura 5: Gráfico das concentrações séricas de colesterol em camundongos tratados com diferentes doses de extrato aquoso de T. subulata ...............................................................37 Figura 6: Gráfico das concentrações séricas de triglicerídeos em camundongos tratados com diferentes doses de extrato aquoso de T. subulata .................................................................................................................................38 Figura 7: Gráfico as concentrações séricas de cálcio em camundongos tratados com diferentes doses de extrato aquoso de T. subulata ...............................................................39 Figura 8: Gráfico as
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