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PRODUCT WATER COCONUT SAP/TODDY DESCRIPTION PRODUCTION VINEGAR PRODUCTION

Coconut vinegar is a natural product obtained from Materials: Materials: a natural process of of coconut sap or 4 cups coconut water 1/4 tsp yeast Fresh coconut sap/toddy coconut water, with no preservatives or chemicals 3/4 1 cup vinegar starter added. Vinegar production involves the Utensils: fermentation of sugar (10-12%) into ethanol Utensils: stainless casserole (alcoholic) by Saccharomyces cerevisiae (yeast) and stainless casserole stainless/wooden spoon stainless ladle the oxidation of ethanol into acetic acid (acetous) stove wide mouth container stove by Acetobacter aceti (bacteria). dry cheesecloth plastic twine/rubber wide large container band dry cheesecloth Sukang tuba is a product of natural alcoholic and rubber tubing plastic twine/rubber band subsequent acetous fermentation of sap from the rubber tubing inflorescence of the coconut palm. Coconut water Procedure: thermometer vinegar is the product of alcoholic fermentation and 1. Strain freshly collected coconut water using a glass bottles with lid acetous fermentation of sugared coconut water cheesecloth. (Sanchez, 2008). 2. Dissolve 3/4 cups sugar in 4 cups coconut water. ° Procedure: 3. Pasteurize by heating at 60-65 C for 10-15 minutes 1. Collect the coconut sap by tapping (making a small Table 1. Physico-chemical Characteristics of Coconut Water Vinegar to kill the microorganisms. incision) on the tender unopened inflorescence. PARAMETER VALUE 4. Transfer in sterilized glass jars, half filled. Cool and For easy collection, the inflorescence is slowly Moisture 98% add 1/4 teaspoon dry yeast. bent downward. 0.1% 5. Cover the container with a clean cheesecloth. 2. Pour the collected sap in a wide large container. Ash 0.3% 6. Allow the sugar solution to ferment for 2-3 weeks Cover with a clean net to allow aeration and or until there are no more bubbles of carbon diox- Total 1.4% prevent contamination. Allow the sap to ferment ide formed to allow alcoholic fermentation. naturally for 60 days. Calcium 24 mg 7. Decant the alcoholic solution to remove the yeast 3. Pasteurize the mixture by boiling for 5-10 minutes 34 mg and other solid materials. Pasteurize the alcoholic at 60-65°C to maintain the desired quality of the Iron 0.1 mg solution and cool immediately. matured vinegar with at least pH 4. Riboflavin 0.01 mg 8. To 4 cups alcoholic solution, add 1 cup vinegar 4. Allow to cool before placing in sterile bottles. starter. Cover with clean cloth/paper to start acetic Source: Coconut as , Banzon, J. et al., 1990 Cover tightly and seal. acid formation. 5. Age the sap vinegar for one year. The color of the Table 2. Physico-chemical Characteristics of Coconut Sap/Toddy Vinegar 9. Set aside for 1 month or until maximum sourness is vinegar changes as it ages, from cloudy white to PARAMETER VALUE obtained. For the development of desirable aroma light yellow to a clear light brown as it further Acidity 5.0-6.0 % and flavor, allow the vinegar to age in barrels or matures. earthen jars which are filled to full capacity. Alcohol 0.5 % 10. Filter the vinegar and then pasteurize before Total Solids 0.77-1.33 % bottling the product. To clear the vinegar, stir it Ash 0.37-0.45 % with well-beaten egg white and heat until egg white Potash 0.15-0.22 % coagulates (optional). Filter vinegar. Density 1.010-1.015

Source: Technical Data Handbook on Coconut, 2013 FPDD Guide No. 8 - Rev. 3, Series of 2019 MOTHER VINEGAR USES AND APPLICATIONS PHILIPPINE PREPARATION  Food preparation As a seasoning for meat, fish, COCONUT Materials: and vegetables on the table or during AUTHORITY 4 cups coconut water 1/4 tsp yeast cooking. 3/4 cups sugar 1 cup Acetobacter  Food preservation Used in the manufacture of vegetable ALBAY RESEARCH Procedure: pickles, catsup and other tomato CENTER A. Alcoholic Fermentation products, , , dre- Perform steps 1-5 on the coconut vinegar pro- ssings, sauces, and additive in many duction and allow to ferment for 4-7 days. manufactured to enhance flavor. COCONUT PROCESSING

 Medicinal use TECHNOLOGIES B. Acetification Used as an antimicrobial agent to treat 1 test tube culture of 3-day old Acetobacter infections. Lowers and aids aceti per 250 ml fermented coconut water in weight management  Cleaning agent 1. Decant the fermented coconut water into two For toilets, kitchen sinks, pots, wood COCONUT sterilized 1000 ml bottle. panels, windows, grills, greasy ovens and hardened paintbrushes. 2. Aseptically add approximately 2 tsp (10 ml)  Deodorizer sterile fermented coconut water to sterile test VINEGAR For refrigerator and microwave ovens tube.

3.Scrape the surface using an inoculating rod and dispense to 1 cup fermented coconut water. STANDARD FOR VINEGAR

4. Add another 2 tsp fermented coconut water, Titratable Acidity = 4% shake to get the remaining organism (A. aceti) (4 grams acetic acid/ 100 mL vinegar) and transfer the same to the batch aseptically in an inoculating chamber or a clean enclosed Source: Philippine Food and Drug Administration room.

5. After 3 days, the implanted fermented coconut water can be used as mother vinegar. Two weeks should be the maximum fermenta- FOOD PRODUCT DEVELOPMENT DIVISION tion period. Albay Research Center Research Development Branch 6. To reproduce mother vinegar, repeat the Philippine Coconut Authority alcoholic fermentation steps and mix this with Banao, Guinobatan, Albay an equal amount of 3-14 day old mother E-mails: [email protected] vinegar. Use this initially prepared mother [email protected] vinegar for the mass production. 0917-558-1447