Anti-OXSR1 Antibody (ARG42121)

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Product datasheet [email protected] ARG42121 Package: 100 μl anti-OXSR1 antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes OXSR1 Tested Reactivity Hu, Ms Tested Application ICC/IF, WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name OXSR1 Antigen Species Human Immunogen Synthetic peptide within aa. 1-100 of Human OXSR1 (NP_005100.1). Conjugation Un-conjugated Alternate Names Serine/threonine-protein kinase OSR1; Oxidative stress-responsive 1 protein; OSR1; EC 2.7.11.1 Application Instructions Application table Application Dilution ICC/IF 1:50 - 1:200 WB 1:500 - 1:2000 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Positive Control Jurkat Calculated Mw 58 kDa Observed Size ~ 56 kDa Properties Form Liquid Purification Affinity purified. Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. Preservative 0.02% Sodium azide Stabilizer 50% Glycerol Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Gene Symbol OXSR1 Gene Full Name oxidative stress responsive 1 Background The product of this gene belongs to the Ser/Thr protein kinase family of proteins. It regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton. [provided by RefSeq, Jul 2008] Function Phosphorylates RELL1, RELL2 and RELT (PubMed:16389068, PubMed:28688764). Phosphorylates PAK1 (PubMed:14707132). Phosphorylates PLSCR1 in the presence of RELT (PubMed:22052202). [UniProt] Cellular Localization Cytoplasm. [UniProt] Images ARG42121 anti-OXSR1 antibody ICC/IF image Immunofluorescence: L929 cells stained with ARG42121 anti-OXSR1 antibody at 1:100 dilution. ARG42121 anti-OXSR1 antibody WB image Western blot: 25 µg of Jurkat cell lysate stained with ARG42121 anti- OXSR1 antibody at 1:1000 dilution. www.arigobio.com 2/2 Powered by TCPDF (www.tcpdf.org).

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OXSR1 (A-4): Sc-271707
SANTA CRUZ BIOTECHNOLOGY, INC. OXSR1 (A-4): sc-271707 BACKGROUND APPLICATIONS Oxidative stress-responsive 1 protein (OXSR1), a protein of 527 amino acids, OXSR1 (A-4) is recommended for detection of OXSR1 of mouse, rat and belongs to the STE20 subfamily. OXSR1 is one of two human homologs of human origin by Western Blotting (starting dilution 1:100, dilution range Fray, a serine/threonine kinase expressed in Drosophila. OXSR1 binds to and 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein phosphorylates p21-activated protein kinase (PAK1) and regulates downstream (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution kinases in response to environmental stress. Endogenous OXSR1 is activated range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution only by osmotic stresses, notably sorbitol and to a lesser extent NaCl. OXSR1 range 1:30-1:3000). may also play a role in regulating the Actin cytoskeleton. The chloride channel Suitable for use as control antibody for OXSR1 siRNA (h): sc-61273, OXSR1 proteins SLC12A1, SLC12A2 and SLC12A6 isoform 2 interact with OXSR1, siRNA (m): sc-61274, OXSR1 shRNA Plasmid (h): sc-61273-SH, OXSR1 but SLC12A4 and SLC12A7 do not. The WNK1 and WNK4 protein kinases shRNA Plasmid (m): sc-61274-SH, OXSR1 shRNA (h) Lentiviral Particles: activate OXSR1 by phosphorlating its T-loop. The OXSR1 protein is widely sc-61273-V and OXSR1 shRNA (m) Lentiviral Particles: sc-61274-V. expressed in mammalian tissues. Molecular Weight of OXSR1: 58 kDa. REFERENCES Positive Controls: OXSR1 (h3): 293 Lysate: sc-158793, HeLa whole cell lysate: 1.
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Page 1 Exploring the Understudied Human Kinome For
bioRxiv preprint doi: https://doi.org/10.1101/2020.04.02.022277; this version posted June 30, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Exploring the understudied human kinome for research and therapeutic opportunities Nienke Moret1,2,*, Changchang Liu1,2,*, Benjamin M. Gyori2, John A. Bachman,2, Albert Steppi2, Rahil Taujale3, Liang-Chin Huang3, Clemens Hug2, Matt Berginski1,4,5, Shawn Gomez1,4,5, Natarajan Kannan,1,3 and Peter K. Sorger1,2,† *These authors contributed equally † Corresponding author 1The NIH Understudied Kinome Consortium 2Laboratory of Systems Pharmacology, Department of Systems Biology, Harvard Program in Therapeutic Science, Harvard Medical School, Boston, Massachusetts 02115, USA 3 Institute of Bioinformatics, University of Georgia, Athens, GA, 30602 USA 4 Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA 5 Joint Department of Biomedical Engineering at the University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC 27599, USA Key Words: kinase, human kinome, kinase inhibitors, drug discovery, cancer, cheminformatics, † Peter Sorger Warren Alpert 432 200 Longwood Avenue Harvard Medical School, Boston MA 02115 [email protected] cc: [email protected] 617-432-6901 ORCID Numbers Peter K. Sorger 0000-0002-3364-1838 Nienke Moret 0000-0001-6038-6863 Changchang Liu 0000-0003-4594-4577 Ben Gyori 0000-0001-9439-5346 John Bachman 0000-0001-6095-2466 Albert Steppi 0000-0001-5871-6245 Page 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.04.02.022277; this version posted June 30, 2020.
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