OSR1 (Phospho Thr185) Antibody Cat

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OSR1 (phospho Thr185) Antibody Cat. No.: 79-859 OSR1 (phospho Thr185) Antibody Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using OSR1 (Phospho-Thr185) antibody (left) or the same antibody preincubated with blocking peptide (right). Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse OSR1 (phospho Thr185) antibody was raised against a peptide sequence around IMMUNOGEN: phosphorylation site of threonine185 (R-K-T (p)-F-V) derived from Human OSR1. TESTED APPLICATIONS: IHC, WB APPLICATIONS: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 The antibody detects endogenous levels of OSR1 only when phosphorylated at threonine SPECIFICITY: 185. PREDICTED MOLECULAR 65 kDa WEIGHT: September 29, 2021 1 https://www.prosci-inc.com/osr1-phospho-thr185-antibody-79-859.html Properties PURIFICATION: Antibodies were purified by affinity-chromatography using epitope-specific peptide. CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM BUFFER: NaCl, 0.02% sodium azide and 50% glycerol. CONCENTRATION: 1 mg/mL STORAGE CONDITIONS: Store antibody at -20˚C for up to one year. Additional Info OFFICIAL SYMBOL: OXSR1 ALTERNATE NAMES: OXSR1, kinase OSR1, Oxidative-stress responsive 1 ACCESSION NO.: NP_005100.1 PROTEIN GI NO.: 4826878 GENE ID: 9943 Background and References Oxidative-stress responsive 1 gene is located in the vicinity of three others genes - GOLGA4, ITGA9 and HYA22 on chromosome 3. These four genes are considered to be BACKGROUND: candidate tumor suppressors. Oxidative-stress responsive 1 protein has similarity to human Ste20/oxidant stress response kinase-1 and is thought to be involved in the response to oxidative stress REFERENCES: 1) Tamari M., J. Hum. Genet. 44:116-120(1999). 2) Kikuno R., DNA Res. 6-197-205(1999). 3) The MGC Project Team; Genome Res. 14:2121-2127(2004). ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 29, 2021 2 https://www.prosci-inc.com/osr1-phospho-thr185-antibody-79-859.html.

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OXSR1 (A-4): Sc-271707
SANTA CRUZ BIOTECHNOLOGY, INC. OXSR1 (A-4): sc-271707 BACKGROUND APPLICATIONS Oxidative stress-responsive 1 protein (OXSR1), a protein of 527 amino acids, OXSR1 (A-4) is recommended for detection of OXSR1 of mouse, rat and belongs to the STE20 subfamily. OXSR1 is one of two human homologs of human origin by Western Blotting (starting dilution 1:100, dilution range Fray, a serine/threonine kinase expressed in Drosophila. OXSR1 binds to and 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein phosphorylates p21-activated protein kinase (PAK1) and regulates downstream (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution kinases in response to environmental stress. Endogenous OXSR1 is activated range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution only by osmotic stresses, notably sorbitol and to a lesser extent NaCl. OXSR1 range 1:30-1:3000). may also play a role in regulating the Actin cytoskeleton. The chloride channel Suitable for use as control antibody for OXSR1 siRNA (h): sc-61273, OXSR1 proteins SLC12A1, SLC12A2 and SLC12A6 isoform 2 interact with OXSR1, siRNA (m): sc-61274, OXSR1 shRNA Plasmid (h): sc-61273-SH, OXSR1 but SLC12A4 and SLC12A7 do not. The WNK1 and WNK4 protein kinases shRNA Plasmid (m): sc-61274-SH, OXSR1 shRNA (h) Lentiviral Particles: activate OXSR1 by phosphorlating its T-loop. The OXSR1 protein is widely sc-61273-V and OXSR1 shRNA (m) Lentiviral Particles: sc-61274-V. expressed in mammalian tissues. Molecular Weight of OXSR1: 58 kDa. REFERENCES Positive Controls: OXSR1 (h3): 293 Lysate: sc-158793, HeLa whole cell lysate: 1.
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Page 1 Exploring the Understudied Human Kinome For
bioRxiv preprint doi: https://doi.org/10.1101/2020.04.02.022277; this version posted June 30, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Exploring the understudied human kinome for research and therapeutic opportunities Nienke Moret1,2,*, Changchang Liu1,2,*, Benjamin M. Gyori2, John A. Bachman,2, Albert Steppi2, Rahil Taujale3, Liang-Chin Huang3, Clemens Hug2, Matt Berginski1,4,5, Shawn Gomez1,4,5, Natarajan Kannan,1,3 and Peter K. Sorger1,2,† *These authors contributed equally † Corresponding author 1The NIH Understudied Kinome Consortium 2Laboratory of Systems Pharmacology, Department of Systems Biology, Harvard Program in Therapeutic Science, Harvard Medical School, Boston, Massachusetts 02115, USA 3 Institute of Bioinformatics, University of Georgia, Athens, GA, 30602 USA 4 Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA 5 Joint Department of Biomedical Engineering at the University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC 27599, USA Key Words: kinase, human kinome, kinase inhibitors, drug discovery, cancer, cheminformatics, † Peter Sorger Warren Alpert 432 200 Longwood Avenue Harvard Medical School, Boston MA 02115 [email protected] cc: [email protected] 617-432-6901 ORCID Numbers Peter K. Sorger 0000-0002-3364-1838 Nienke Moret 0000-0001-6038-6863 Changchang Liu 0000-0003-4594-4577 Ben Gyori 0000-0001-9439-5346 John Bachman 0000-0001-6095-2466 Albert Steppi 0000-0001-5871-6245 Page 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.04.02.022277; this version posted June 30, 2020.
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