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Datasheet A11886-3 Anti-NDUFB10 Antibody

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Datasheet A11886-3 Anti-NDUFB10 Antibody Product datasheet Anti-NDUFB10 Antibody Catalog Number: A11886-3 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Basic Information Product Name Anti-NDUFB10 Antibody Gene Name NDUFB10 Source Rabbit IgG Species Reactivity human, mouse, rat Tested Application WB,IHC-P,ICC/IF,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human NDUFB10 recombinant protein (Position: D36-S172). Purification Immunogen affinity purified. Observed MW 22KD Dilution Ratios Western blot: 1:1000-5000 Immunohistochemistry in paraffin section IHC-(P)： 1:50-400 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing Background Information NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 is an enzyme that in humans is encoded by the NDUFB10 gene. The protein encoded by this gene is an accessory subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I) that is not directly involved in catalysis. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein complex has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.[7] Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix. Reference FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-NDUFB10 Antibody Catalog Number: A11886-3 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Anti-NDUFB10 Antibody被引用在0文献中。 暂无引用 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-NDUFB10 Antibody Catalog Number: A11886-3 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Selected Validation Data Figure 1. Western blot Figure 2. IHC analysis analysis of anti- using anti- NDUFB10 NDUFB10 antibody antibody (A11886-3). (A11886-3). The sample detected in paraffin- well of each lane was embedded section of loaded with 50ug of mouse skeletal muscle sample under reducing tissue. Biotinylated goat conditions. Lane 1: anti-rabbit IgG was used human HELA whole cell as secondary antibody. lysates, Lane 2: human The tissue section was HEPG2 whole cell lysates, developed using Strepavi Lane 3: human Jurkat din-Biotin-Complex whole cell lysates, Lane 4: (SABC) (Catalog # human HEK293 whole SA1022) with DAB as the cell lysates, Lane 5: chromogen. human MCF-7 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: human HL-60 whole cell lysates, Lane 9: rat liver tissue lysates, Lane 10: mouse liver tissue lysates. Use rabbit anti- NDUFB10 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for NDUFB10 at approximately 22KD. The expected band size for NDUFB10 at 22KD. Figure 3. IHC analysis Figure 4. IHC analysis using anti- NDUFB10 using anti- NDUFB10 antibody (A11886-3). antibody (A11886-3). detected in paraffin- detected in paraffin- embedded section of rat embedded section of skeletal muscle tissue. human prostate cancer Biotinylated goat anti- tissue. Biotinylated goat rabbit IgG was used as anti-rabbit IgG was used FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3 Product datasheet Anti-NDUFB10 Antibody Catalog Number: A11886-3 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] secondary antibody. The as secondary antibody. tissue section was The tissue section was developed using Strepavi developed using Strepavi din-Biotin-Complex din-Biotin-Complex (SABC) (Catalog # (SABC) (Catalog # SA1022) with DAB as the SA1022) with DAB as the chromogen. chromogen. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 4 Powered by TCPDF (www.tcpdf.org).
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