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1 1 a Transgenic Line That Reports CSF1R Protein Expression bioRxiv preprint doi: https://doi.org/10.1101/2020.07.09.196402; this version posted July 9, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 2 A transgenic line that reports CSF1R protein expression provides a definitive marker for 3 the mouse mononuclear phagocyte system. 4 5 Kathleen Grabert2*, Anuj Sehgal1*, Katharine M. Irvine1*, Evi Wollscheid-Lengeling2, Derya 6 D. Ozdemir2, Jennifer Stables1, Garry A. Luke3, Martin D. Ryan3, Antony Adamson4, Neil E. 7 Humphreys4, Cheyenne J. Sandrock1, Rocio Rojo5, Veera A. Verkasalo6, Werner Mueller4, 8 Peter Hohenstein2, Allison R. Pettit1, Clare Pridans*6 and David A. Hume*#1. 9 10 1. Mater Research Institute-University of Queensland, Translational Research Institute, 11 Woolloongabba, Brisbane, Qld 4102, Australia 12 2. The Roslin Institute, University of Edinburgh, Easter Bush, Midlothian EH259RG, Scotland, UK. 13 3. School of Biology, University of St Andrews, North Haugh, St Andrews, Scotland, UK 14 4. Faculty of Biology, Medicine & Health, School of Biological Sciences, Manchester, M13 9PT, 15 UK 16 5. Tecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, C.P. 64710, Monterrey, 17 N.L., Mexico 18 6. University of Edinburgh Centre for Inflammation Research, Little France, Edinburgh EH16 4TJ, 19 UK 20 # Corresponding author: [email protected] 21 22 23 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.09.196402; this version posted July 9, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 24 Abstract 25 The proliferation, differentiation and survival of cells of the mononuclear phagocyte system (MPS, 26 progenitors, monocytes, macrophages and classical dendritic cells) is controlled by signals from the 27 macrophage colony-stimulating factor receptor (CSF1R). Cells of the MPS lineage have been 28 identified using numerous surface markers and transgenic reporters but none is both universal and 29 lineage-restricted. Here we report the development and characterization of a novel CSF1R reporter 30 mouse. A Fusion Red (FRed) cassette was inserted in-frame with the C-terminus of CSF1R, 31 separated by a T2A-cleavable linker. The insertion had no effect of CSF1R expression or function. 32 CSF1R-FRed was expressed in monocytes and macrophages and absent from granulocytes and 33 lymphocytes. In bone marrow, CSF1R-FRed was absent in lineage-negative hematopoietic stem 34 cells (HSC), arguing against a direct role for CSF1R in myeloid lineage commitment. It was highly- 35 expressed in marrow monocytes and common myeloid progenitors (CMP) but significantly lower 36 in granulocyte-macrophage progenitors (GMP). In sections of bone marrow, CSF1R-FRed was also 37 detected in osteoclasts, CD169+ resident macrophages and, consistent with previous mRNA 38 analysis, in megakaryocytes. In lymphoid tissues, CSF1R-FRed highlighted diverse MPS 39 populations including classical dendritic cells. Whole mount imaging of non-lymphoid tissues in 40 mice with combined CSF1R-FRed/Csf1r-EGFP confirmed the restriction of CSF1R expression to 41 MPS cells. The two markers highlight the remarkable abundance and regular distribution of tissue 42 MPS cells including novel macrophage populations within tendon and skeletal muscle and 43 underlying the mesothelial/serosal/capsular surfaces of every major organ. The CSF1R-FRed mouse 44 provides a novel reporter with exquisite specificity for cells of the MPS. 45 Key words: macrophage, dendritic cell, markers, imaging, CSF1R, progenitors, myelopoiesis. 46 47 48 2 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.09.196402; this version posted July 9, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 49 Introduction 50 The proliferation, differentiation and survival of most tissue macrophages depends upon 51 signals from the macrophage colony-stimulating factor receptor (CSF1R). Homozygous 52 recessive mutations in the receptor in mice, rats and humans lead to loss of tissue macrophages 53 and osteoclasts and pleiotropic developmental abnormalities in many organs systems 54 (reviewed in (1)). Csf1r mRNa is expressed by all resident tissue macrophages (2). The 55 transcriptional regulation of the Csf1r gene has been studied extensively as a model of 56 macrophage differentiation (3). Deletion of a conserved enhancer in the first intron (Fms 57 intronic regulatory element, FIRE) leads to selective loss of Csf1r expression in tissue 58 macrophage populations including microglia in the brain (4). Transgenic reporters containing 59 FIRE have been generated in mice (5, 6) rats (7), chickens (8, 9) and sheep (10) and in each 60 species highlight the location and abundance of macrophage populations in every organ. 61 However, there are caveats to the utility and interpretation of these Csf1r reporters. Firstly, 62 based upon the phenotype of the Csf1rFIRE/FIRE mice, we identified regulatory elements 63 outside of the transgene construct, so that the absence of reporter gene detection in particular 64 cells may be difficult to interpret (4). In the chick, we found that Kupffer cells expressed Csf1r 65 mRNA and protein but did not express the Csf1r-mApple reporter transgene (11). Conversely, 66 mouse and human granulocytes express Csf1r mRNA but do not translate the protein (12) and 67 the Csf1r promoter also has detectable activity in B lymphocytes, which share expression of 68 the transcriptional regulator PU.1 (13, 14). Accordingly, the Csf1r reporter transgenes in 69 mouse, rat and chicken were detectable in both granulocytes and B cells. In many tissues this 70 is not a major issue; the macrophages have a characteristic location and stellate morphology 71 (15, 16). But in lymphoid tissues, and mucosal surfaces and inflammatory sites, it would be 72 desirable to separate these cell types. 3 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.09.196402; this version posted July 9, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 73 There have been a number of reports on expression of CSF1R in non-hematopoietic cells 74 including neurons and neuronal progenitors in the brain and epithelial cells in the gut and 75 kidney (17-19). In each case, the conclusion depends upon immunolocalization with poorly- 76 characterized polyclonal antibodies or conditional reporter transgenes and was not supported 77 by subsequent analyses (1). In the brain, the mouse Csf1r-EGFP reporter transgene and the 78 protein detected by anti-CD115 antibody were expressed exclusively in microglia (20, 21). A 79 further argument against functional expression of CSF1R in non-hematopoietic cells follows 80 from the complete rescue of the pleiotropic impacts of Csf1r knockouts in mice (22) by 81 postnatal adoptive transfer of wild-type bone marrow (BM) cells. 82 By contrast to surface markers such as F4/80, CD11b/c, CD163, CD68, CD169, CD64, CD206, 83 CX3CR1, LYVE1 and MHCII, which are expressed independently by subsets of tissue MPS 84 cells (2, 15), CSF1R is a potential universal marker for cells of the mononuclear phagocyte 85 system (MPS). There are available monoclonal antibodies against mouse CSF1R protein 86 (CD115). Two different antibodies have been described that block CSF1 binding to the mouse 87 receptor and can deplete tissue macrophages (23, 24). Anti-CD115 antibodies can detect cell 88 surface expression on at least some myeloid progenitors in the BM (25) and on isolated blood 89 monocytes (6) and can compete for binding of labelled CSF1 protein to tissue macrophages in 90 vivo (5). However, CSF1R protein is not readily detected on most tissue macrophages by 91 immunohistochemistry using monoclonal anti-CD115. The lack of detection is most likely a 92 consequence of competition with endogenous ligand (the available antibodies compete for 93 CSF1 binding) and the down-regulation of the receptor from the cell surface by ligand. CSF1 94 is internalized by receptor-mediated endocytosis and both receptor and ligand are degraded 95 (26). Accordingly, signaling requires continuous synthesis of new receptors. The surface 96 receptor is also subject to rapid proteolytic cleavage in response to signals that activate 97 macrophages (27, 28) and it is likely cleaved from the surface directly or indirectly during 4 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.09.196402; this version posted July 9, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 98 tissue disaggregation to isolate macrophages. All cell isolation procedures lead to activation of 99 inflammatory genes in macrophages (2). 100 Classical dendritic cells (cDC) were originally defined by their unique ability to present antigen 101 to naïve T cells. Class II MHC+ migratory monocytes, monocyte-derived cells and subsets of 102 resident tissue macrophages also possess active antigen presentation activity (29-31). cDC and 103 monocytes share a committed progenitor in the BM that expresses CSF1R(32). cDC have been 104 classified separately from macrophages in different tissues and contexts based upon the lack of 105 certain surface markers, including CD64 (Fcgr1) and MERTK (33).
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