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䖜 cp4 epsps สഐ㙀䲔㥹ࡲ⦹㊣ MON 87427 䘋ਓ⭘ ࣐ᐕᯉⲴᆹޘ䇱Җ
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亩ⴞ〠˖ 䖜 cp4 epsps สഐ㙀䲔㥹ࡲ⦹㊣ MON 87427 䘋ਓ⭘࣐ ᐕᯉⲴᆹޘ䇱Җ
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ᵜ⭣䈧ҖਇѝॾӪ≁ޡ઼ഭ㪇ᵳ⌅ǃц⭼⸕䇶ӗᵳ㓴㓷˄WIPO˅㪇ᵳޜ㓖઼ ˈᣔDŽᵜ⭣䈧Җ৺ަ䱴ᑖᶀᯉӵ᧕᭦Ⲵ⌅㿴ᵪᶴᡰ⭘؍㓖Ⲵޜ૱WIPO 㺘╄৺ᖅ丣ࡦ ᒦӵ⭘Ҿ᭟ᤱᆏኡ䜭ޜਨⲴᓄ⭣䈧DŽ䲔䶎ޣ⌅ᖻ⌅㿴৺⌅㿴ѫ㇑䜘䰘㿴ᇊˈަԆ ԫօሩᵜ⭣䈧Җ৺ަ䱴ᑖ䍴ᯉⲴަԆᓄ⭘ǃᤧ䍍৺वᤜӂ㚄㖁ޜᐳ൘ⲴՐ䜭ᗵ享 ਨⲴҖ䶒਼DŽᵜ⭣䈧ѝ᧿䘠Ⲵᡆ䱴ᑖⲴ⸕䇶ӗᵳǃؑԕ৺ᶀᯉޜһݸᗇࡠᆏኡ䜭 ᐢ㓿⭡ᆏኡ䜭ޜਨ⭣ᣕу࡙ᡆ㘵㧧ᗇу࡙ˈѪᆏኡ䜭ޜਨᡰᴹDŽᆏኡ䜭ޜਨᨀӔᵜ⭣ ਨሶᵜ⭣䈧Җѝ᧿䘠ᡆवਜ਼Ⲵᶀᯉǃؑᡆ⸕䇶ӗᵳᦸᵳ㔉ަԆޜ䈧Җн㺘⽪ᆏኡ䜭 ԫօњӪᡆᇎփDŽ ມ ߏ 䈪 ᱄ ㇗⨼ᶗׁɆȽɅߒѐ䖢ޞ1κ൞ມߏ⭩ᣛҜҁࢃθᓊ䇚ⵕ䰻䈱Ʌߒѐ䖢ะഖ⭕⢟ᆿ ะഖ⭕⢟ᆿޞ䇺ԭ㇗⨼ࣔ⌋ɆȽɅߒѐ䖢ะഖ⭕⢟䘑ਙᆿޞ㇗⨼ࣔ⌋Ɇㅿᴿީ⌋㿺θ Ҽ䀙ީ㾷≸Ⱦ 2κ↚⭩ᣛҜੂṭ䘸⭞ӄ⌋㿺㿺ᇐⲺᣛ㊱ᇔ僂⹊ガૂѣ䰪䈋僂Ⲻᣛθ〦࠼ࡡ ᭯Ѱ“ߒѐ䖢ะഖ⭕⢟ᇔ僂⹊ガᣛҜ ”ૂ“ߒѐ䖢ะഖ⭕⢟ѣ䰪䈋僂ᣛҜ ”Ⱦ 3κ⭩ᣛҜᇯᓊᖉऻᤢԛс䜞࠼φ⭩䈭㺞Ƚ亯ⴤᇯ㾷Ƚᐛ֒ⴤⲺૂᝅѿȽള ཌ⹊ガⲺީ㜂Ქ䍺ᯏȽᆿޞᙝ䇺ԭȽ䈋僂ᯯṾȽީ䱺Ԭ䍺ᯏȽᵢঋփߒѐ䖢ะ ഖ⭕⢟ᆿޞቅ㓺ᇗḛᝅ㿷Ƚᵢঋփᇗḛᝅ㿷Ƚᡶ൞ⴷδᐸȽ㠠⋱॰εⲺߒѐ㺂᭵ѱ㇗ 䜞䰞Ⲻᇗḛᝅ㿷Ⱦ 4κ⭩䈭ᇔ僂⹊ガⲺθ⭩䈭㺞㺞 2 ມߏθ亯ⴤ〦ᓊऻ䖢ะഖᙝ⣬Ƚ։⭕⢟ 〦Ƚᇔ僂⹊ガᡶ൞ⴷδᐸȽ㠠⋱॰ε〦ૂᇔ僂⹊ガ䱬⇫ㅿᇯθྸ “䖢ะഖᣍ㲡ỿ 㣧൞⋩्ⴷⲺᇔ僂⹊ガ ”ȾжԳ⭩ᣛҜਠ㜳ऻੂж⢟〃Ⲻ։⭕⢟ૂੂⲺ䖢ะഖᙝ ⣬Ⱦ“ᆿޞᙝ䇺ԭ ”ਥуມߏȾ “䈋僂ᯯṾ”ऻᤢ⹊ガⴤḽȽཌⓆะഖⲺ〦ૂᶛⓆȽ䖳 ։ᶺᔰഴ䉧Ƚ䖢ौᯯ⌋ૂ㿺⁗Ƚ൦⛯Ƚᆿޞ᧠᯳ㅿȾ “ީ䱺Ԭ䍺ᯏ ”ऻᤢ⌋Ӱ䇷 Ҝૂ㩛ѐᢝ➝ⲺགྷদԬθ 㤛⎿ਾ֒亯ⴤθ ᓊᨆӚਂᯯਾ֒ᡌ䖢䇟অ䇤ㅿȾ “ᡶ൞ⴷδᐸȽ 㠠⋱॰εⲺߒѐ㺂᭵ѱ㇗䜞䰞Ⲻᇗḛᝅ㿷 ”ਥуມߏθ⭩ᣛᶆᯏᣛ䘷ߒѐ䜞ᰬθᢺ䘷ᇔ 僂⹊ガᡶ൞ⴷδᐸȽ㠠⋱॰εⲺߒѐ㺂᭵ѱ㇗䜞䰞Ⱦ 5κ⭩䈭ѣ䰪䈋僂Ⲻθ⭩䈭㺞㺞 2 ມߏȾ⭩ᣛҜѣ“ᆿޞᙝ䇺ԭ ”Ƚ“䈋僂ᯯṾ ”ૂ 䇺ԭ㇗⨼ࣔ⌋Ɇ䱺ᖋ IȽIIȽIII 㾷≸䙆ᶗມޞɅߒѐ䖢ะഖ⭕⢟ᆿ➝ד” ީ䱺Ԭ䍺ᯏ“ ߏȾ“ᡶ൞ⴷδᐸȽ㠠⋱॰ε㓝ߒѐ㺂᭵ѱ㇗䜞䰞Ⲻᇗḛᝅ㿷 ”ਥуມߏθ⭩ᣛᶆᯏᣛ 䘷ߒѐ䜞ᰬθᢺ䘷ѣ䰪䈋僂ᡶ൞ⴷδᐸȽ㠠⋱॰ε㓝ߒѐ㺂᭵ѱ㇗䜞䰞Ⱦ 6κ⭩䈭⧥ູ䠀᭴ૂ⭕ӝᙝ䈋僂Ⲻθ⭩䈭㺞㺞 2 ມߏχ⭩ᣛҜѣ “ᆿޞᙝ䇺ԭ ”Ƚ 䇺ԭ㇗⨼ࣔ⌋Ɇ䱺ᖋ IȽIIȽޞɅߒѐ䖢ะഖ⭕⢟ᆿ➝ד” 䈋僂ᯯṾ ”ૂ“ީ䱺Ԭ䍺ᯏ“ III 㾷≸䙆ᶗມߏȾሯӄᐨ䗽Ⲻ⧥ູ䠀᭴ᡌ⭕ӝᙝ䈋僂θ൞䈋僂㔉ᶕθ㤛ੂж䖢 ะഖ⭕⢟൞൦⛯ԛੂ㿺⁗䠃གྷ䈋僂θ൞⭩ᣛᰬ “ᆿޞᙝ䇺ԭ ”䜞࠼ਥⴷ⮛Ⱦ 7κ⭩䈭ߒѐ䖢ะഖ⭕⢟ᆿޞ䇷ҜⲺθ⭩䈭㺞㺞 3 ມߏχ⭩ᣛҜѣ “ᆿޞᙝ䇺ԭ ” 䇺ԭ㇗⨼ࣔ⌋Ɇ䱺ᖋ IȽIIȽIII 㾷≸䙆ᶗޞɅߒѐ䖢ะഖ⭕⢟ᆿ➝ד” ީ䱺Ԭ䍺ᯏ“ૂ ມߏθ“䈋僂ᯯṾ ”у⭞ມߏχ 䖢ะഖὃ⢟Ⲻ〃ળᓊㅜਾ Ʌߒѐὃ⢟〃ળ㿺ᇐɆ χ Ʌߒѐ䖢ะഖב䇷ҜⲺθ䴶ᨆޞ俌⅗⭩䈭ߒѐ䖢ะഖ⭕⢟〃δᡌ㌱ε⭕ӝᓊ⭞ᆿ ⭕⢟〃δᡌ㌱ε⭕ӝᓊ⭞㔲ਾ䇺ԭᣛɆθѱ㾷ऻᤢሯᡇള⭕ӝȽ䍮᱉Ƚ⽴Րㅿ ᯯ䶘ᖧଃȾ 䇷ҜⲺθ൞⭩䈭ᡠ↘ᰛ 30 Ѡᐛ֒ޞ8κ俌⅗⭩䈭ߒѐ䖢ะഖ⭕⢟⭕ӝᙝ䈋僂ૂᆿ ᡶ⭩ᣛ䖢ะഖ⭕⢟⍱ᙝṭૂᢶᵥ䍺ᯏȾṭ㾷≸φㅜਾ㾷≸Ⲻθᴿ⍱ᙝⲺבᰛࢃᨆ ὃ⢟〃ᆆ 2.5 kgθࣞ⢟ 5ml 㹶ṭᡌ 3g 㓺㓽пԳθᗤ⭕⢟ 3 ⅗θ 10 ㇗ṭȾᢶ ཌⓆ⡽⇫᮪ਾ䘑ะഖ㓺Ⲻ Southern ᵸӚ㔉᷒Ƚ䍓בᵥ䍺ᯏ㾷≸φ⭩䈭⭕ӝᙝ䈋僂ᨆ ⌊ؗᚥ䖢ौ։⢯ᔸᙝ PCR Ỷ⎁ᯯ⇫⡽ޛཌⓆᨈב䇷ҜᨆޞᮦỶ⎁ᯯ⌋ㅿχ ⭩䈭ᆿ ㅿȾ 9κ⭩ᣛҜᓊᖉ⭞ѣᮽມߏθжᕅॷԳθжᗁֵ⭞ A4 㓮θ↙ᮽ⭞ቅᇁ։ᢉদθ ݿⴎȾሯӄуㅜਾ㾷≸Ⲻ⭩ᣛҜθуҾ⨼Ⱦב㺂䐓θᒬᨆكḽᆍ䰪䐓ૂঋ 10κ⭩䈭㘻ਥԛ⌞᱄ଠӑ䍺ᯏ䴶㾷ؓᇼᒬ䈪᱄⨼⭧Ⱦ 11κ⨼ᰬ䰪φ∅ᒪ 3 ⅗θᡠ↘ᰛᵕ࠼ࡡѰ 3 ᴾνᰛȽ 7 ᴾ 1 ᰛૂ 11 ᴾ 1 ᰛȾ 12κ⨼ঋփφߒѐ䜞㺂᭵ᇗ㔲ਾࣔޢᇚ〇ᮏネਙȾ൦൶φ्Ӣᐸᵓ䱩॰ߒኋ 侼䠂 11 ਭȾ䛤᭵㕌⸷φ 100125Ⱦ᭬ⅴঋփφߒѐ䜞〇ᢶਇኋѣᗹχᔶᡭ㺂φѣؗ䬬 㺂्Ӣᵓ䱩᭥㺂χᑆਭφ 7111110189800000419Ⱦ ⴞ ᖅ
аǃ⭣䈧㺘...... 1 Ҽǃ亩ⴞᇩ᪈㾱...... 9 йǃᐕⴞⲴ઼ѹ ...... 12 ഋǃഭཆޣ⹄ウⲴ㛼Ჟ䍴ᯉ ...... 13 ӄǃ䖜สഐἽ⢙Ⲵᆹޘᙗ䇴ԧ ...... 20 1. ։ὃ⢟Ⲻᆿޞᙝ䇺ԭ...... 20 ᙝ䇺ԭ...... 35ޞะഖᬃ֒Ⲻᆿ .2 3. 䖢ะഖὃ⢟ᆿޞᙝ䇺ԭ...... 68 4. 䖢ะഖὃ⢟ӝᆿޞᙝ䇺ԭ...... 128 ޝǃޣ䱴Ԧ䍴ᯉ...... 141 1. ⴤⲺะഖⲺṮ㤭䞮ᓅࡍެ᧞ሲⲺ≞ะ䞮ᓅࡍ...... 142 2. ⴤⲺะഖф䖳։ᶺᔰⲺഴ䉧...... 143 3. ⴤⲺะഖфὃ⢟ะഖ㓺᮪ਾެ㺞䗴Ⲻ࠼ᆆỶ⎁ᡌ䢪ᇐ㔉᷒...... 146 4. 䖢ะഖᙝ⣬ӝ⢟ⲺỶ⎁ૂ䢪ᇐᢶᵥ...... 171 5. 䈋僂䱬⇫ᇗҜⲺགྷদԬ...... 174 6. 䈋僂䱬⇫Ⲻᆿޞᙝ䇺ԭ䈋僂ᙱ㔉ᣛ...... 174 7. 䖢ะഖὃ⢟ሯ⭕ᘷ⧥ູᆿޞᙝⲺ㔲ਾ䇺ԭᣛ...... 174 8. 伕ᆿޞᙝⲺ㔲ਾ䇺ԭᣛ...... 174 9. 䈛㊱䖢ะഖὃ⢟ളཌ⭕ӝᓊ⭞Ᾰ߫...... 182 10. ⭦䰪ⴇᯯṾ...... 268 11. ᇗḛᡶ䴶Ⲻެᆹީ䍺ᯏ ...... 269 䖢ะഖ⭕⢟൞䗉࠰ളᇬᡌ൦॰ᐨ㔅ݷ䇮֒Ѱᓊ⭞䙊ᒬᣋ᭴ᐸ൰Ⲻ .12 䇷᱄ᮽԬ...... 271 13. 䗉࠰ളᇬᡌ൦॰㔅䗽〇ᆜ䈋僂䇷᱄ሯӰ㊱Ƚࣞὃ⢟ૂ⭕ᘷ⧥ູᰖᇩⲺ 䍺ᯏ...... 290 гǃᵜঅսߌъ䖜สഐ⭏⢙ᆹޘሿ㓴ᇑḕ㿱...... 292 ޛǃᵜঅսᇑḕ㿱 ...... 293 ҍǃᡰ൘ⴱ˄ᐲǃ㠚⋫४˅Ⲵߌъ㹼᭯ѫ㇑䜘䰘Ⲵᇑḕ㿱...... 294 ߌъ䜘ޣҾMON 87427ᆹޘ䇴ԧ⭣䈧Ⲵഎ༽࠭ ˄2017ᒤ6ᴸ12ᰕ˅
I ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ҿߌъ䜘ᢩ༽ؑ࠭Ⲵ䈤᰾ޣਨޜᆏኡ䜭
䰞仈˖ሩҾ 2016 ᒤ 12 ᴸᢩ༽᮷ԦⲴഎ༽ᇩѝˈᵚᨀ㖾ഭᐢਁ⧠Ⲵ 17 㥹 ⭈㟖ᣇᙗᵲ㥹Ⲵ〠ǃᣇᙗᵲ㥹Ⲵ䲔㥹ࡲ⭘䟿৺ަ↻⮉䟿ਈॆDŽ 䈤᰾˖ᵲ㥹ሩ䲔㥹ࡲⲴᣇᙗаⴤᱟߌъ⭏ӗѝⲴаབྷ䰞仈DŽሩҾԫօа䲔㥹ࡲ 㘼䀰ˈ৽༽䮯ᵏঅа֯⭘㘼н䖵ԕަԆᵲ㥹᧗ࡦ⇥ᴰ㓸䜭Պሬ㠤ᣇᙗᵲ㥹㗔փ Ⲵӗ⭏DŽᵲ㥹Ⲵ䲔㥹ࡲᣇᙗ䰞仈㠚кц㓚ӄॱᒤԓ䎧ቡᕅ䎧Ҷޣ⌘ˈᒦ䶎ᱟ䲿⵰ 㙀䲔㥹ࡲ䖜สഐ⢙㘼ࠪ⧠Ⲵᯠ䰞仈DŽ ⴞࡽ൘㖾ഭᣕਁ⧠Ⲵ㥹⭈㟖ᣇᙗᵲ㥹ޡ 17 ˈަ〠䈧㿱л㺘DŽ㔏䇑ᮠ ᦞᶕⓀҾഭ䱵䲔㥹ࡲᣇᙗᵲ㥹䈳ḕ㖁ㄉ http://www.weedscience.org/DŽ
бᆖ 㤡᮷ ѝ᮷ ਁ⧠ൠ⢙㊫ර 1 Amaranthus palmeri Palmer 䮯㣂㣻 ⦹㊣ǃỹ㣡ǃབྷ䉶 Amaranth 2 Amaranthus spinosus Spiny Amaranth ࡪ㣻 ỹ㣡 3 Amaranthus Tall Waterhemp ㌉᷌㣻 ⦹㊣ǃỹ㣡ǃབྷ䉶ǃ tuberculatus ˄=㾯䜘㣻˅ 儈㋡ (=A.rudis) 4 Ambrosia Common 䊊㥹 ⦹㊣ǃỹ㣡ǃབྷ䉶 artemisiifolia Ragweed 5 Ambrosia trifida Giant Ragweed й㻲ਦ䊊㥹 ⦹㊣ǃỹ㣡ǃབྷ䉶 6 Conyza bonariensis Hairy Fleabane 俉э㥹 ⦹㊣ǃ㪑㨴ǃ᷌ഝ 7 Conyza canadensis Horseweed ሿ㬜㥹 ⦹㊣ǃỹ㣡ǃབྷ䉶ǃ ሿ哖ǃᵿӱ Echinochloa colona Junglerice ݹཤぇ ⦹㊣ǃỹ㣡ǃ㪑㨴ǃ 8 ᷌ഝ 9 Eleusine indica Goosegrass ⢋ㅻ㥹 ỹ㣡ǃབྷ䉶ǃ䉧⢙ 10 Helianthus annuus Common ੁᰕ㪥 ⦹㊣ Sunflower 11 Kochia scoparia Kochia ൠ㛔 ⦹㊣ǃỹ㣡ǃབྷ䉶ǃ ⭈㭇 12 Lolium perenne ssp. Italian Ryegrass ཊ㣡唁哖㥹 ⦹㊣ǃỹ㣡ǃབྷ䉶ǃ multiflorum ሿ哖ǃ᷌ഝǃ㪑㨴ǃ 㤌㬯 13 Lolium rigidum Rigid Ryegrass ⺜ⴤ唁哖㥹 ᵿӱǃ᷌ഝ 14 Poa annua Annual ᰙ⟏ ᵿӱǃ㥹ක Bluegrass 15 Parthenium Ragweed 䬦㜦㧺 䶎⢙Ἵ४˄ྲ
II ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 hysterophorus Parthenium 䐟䗩ǃ䫱䖘˅ 㖇ᯟ㬏 ሿ哖״ Salsola tragus Russian-thistle 16 17 Sorghum halepense Johnsongrass ⸣㤵 བྷ䉶
к㺘ѝᡰࡇⲴ㥹⭈㟖ᣇᙗᵲ㥹བྷ䜘࠶ᱟ൘䮯ᵏԕḷㆮࡲ䟿௧ᯭ㥹⭈㟖Ⲵߌ ⭠ѝਁ⧠Ⲵˈ㥹⭈㟖Ⲵާփ௧ᯭࡲ䟿ഐߌ⭠ѝἽⲴ⢙㘼ᔲˈঅ⅑௧ᯭᴰ儈ࡲ 䟿ਟ䗮 1.5 lbs. a.e./A.DŽл㺘ѝᾲ䘠Ҷн਼ᣇ㥹⭈㟖⢙Ⲵḷㆮ௧ᯭᴰ儈ࡲ䟿˖
অ⅑௧ᯭᴰ儈ࡲ䟿 ᙫ௧ᯭᴰ儈ࡲ䟿 ᣇ㥹⭈㟖⢙ ˄⢙㹼䰤/䎺亦௧ᯭ˅ ˄⢙㹼䰤/䎺亦௧ᯭ˅ (lb. a.e./A) (lb. a.e./A) ᣇ㥹⭈㟖㤌㬯 1.55 4.61 ᣇ㥹⭈㟖⋩㨌 0.56 0.77 ˄᱕⋩㨌˅ ᣇ㥹⭈㟖⋩㨌 0.77 1.55 ˄ߜ⋩㨌˅ ᣇ㥹⭈㟖⋩㨌 1.55 1.55 ˄MON 88302˅ ᣇ㥹⭈㟖བྷ⭠⦹ 1.125 2.25 ㊣˄NK603˅ 0.77˄᭦㧧ࡽ௧ᯭ˅ 2.32˄ྲ᷌᭦㧧ࡽ࣐௧а⅑˅ ᣇ㥹⭈㟖⭌⦹㊣ 1.55 4.64 ˄NK603˅ ᣇ㥹⭈㟖ỹ㣡 0.77 2.8125 ˄MON 1445˅ 1.125˄ӊ࡙ẁ䛓ᐎ˅ 4.359˄ྲ᷌᭦㧧ࡽ࣐௧а⅑˅ 1.55˄᭦㧧ࡽ௧ᯭ˅ ᣇ㥹⭈㟖ỹ㣡 1.125 ˄MON 88913˅ 1.55˄ӊ࡙ẁ䛓ǃᯠ໘㾯 4.50 ˅କᐎǃ㾯ᗧݻ㩘ᯟᐎ˅ 6.05˄ྲ᷌᭦㧧ࡽ࣐௧а⅑ 1.55˄᭦㧧ࡽ௧ᯭ˅ ᣇ㥹⭈㟖བྷ䉶 1.55 2.25 ˄MON 89788˅ ᣇ㥹⭈㟖⭌㨌 1.125 3.5156
ᵲ㥹Ⲵ䲔㥹ࡲᣇᙗ㻛ᇊѹѪḀ䲔㥹ࡲⴞḷᵲ㥹ѝⲴḀа㗔փ㧧ᗇҶ㙀ਇ 䘉䲔㥹ࡲⲴ㜭࣋ˈ൘ԕᖰⲴ௧ᯭࡲ䟿л䈕䲔㥹ࡲሩ⢩ᇊ㗔փᵲ㥹нᴹ᭸ ˄WSSA, 2011˗HRAC, 2016˅ˈࡽ㺘ѝᡰࡇᵲ㥹ণᱟ൘ḷㆮࡲ䟿௧ᯭਾᆈ⍫Ⲵ ᣇᙗ㗔փDŽ᤹➗ഭ䱵䲔㥹ࡲᣇᙗᵲ㥹䈳ḕ㖁ㄉⲴ༠᰾ˈᨀࡠ䈕㖁ㄉᒦ༠〠Ѫ“ᣇ ᙗᵲ㥹”Ⲵ㗔փᗵ享㓿䗷䘋а↕Ⲵ⍻䈅ˈ᧘㦀᧗ࡦᶑԦ˄ษޫ㇡ǃᇔ˅лⲴἽ Ṛࡲ䟿૽ᓄ䈅傼˄http://www.weedscience.org/Documents/ResistanceCriterion.pdf˅DŽ 䘹㗔փ࠶࡛⭘վҾ઼儈ҾḷㆮⲴࡲ䟿௧ᯭⴞḷ䲔ى൘ࡲ䟿૽ᓄ⹄ウѝˈᣇᙗᵲ㥹 III ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㥹ࡲˈ䇴ՠᵲ㥹ἽṚⲴᆈ⍫㺘⧠DŽࡽ㺘ѝᡰࡇ㖁ㄉѝⲴᵲ㥹ᮠᦞ⭡н਼Ⲵ⹄ウ㘵 ᨀˈަ⹄ウᰦ֯⭘Ⲵ௧ᯭࡲ䟿ҏнቭ਼DŽ∄ྲˈNandula ㅹӪሩ㌉᷌㣻 ˄Amaranthus tuberculatus˅䘋㹼Ⲵ⹄ウѝˈ㥹⭈㟖௧ᯭࡲ䟿㤳തѪ 0.21-3.36 kg a.e. ha-1˄http://www.bioone.org/doi/full/10.1614/WS-D-12-00155.1˅ˈ㘼 Bell ㅹӪ ਼ṧሩ㌉᷌㣻䘋㹼Ⲵ⹄ウѝˈ㥹⭈㟖Ⲵ௧ᯭࡲ䟿㤳തࡉѪ 0.052 to 6.7 kg a.e. ha-1 http://pubs.acs.org/doi/abs/10.1021/jf103797n˅DŽє亩⹄ウ൷㺘᰾ˈণ֯൘⍻䈅˄ ᡰ⭘ᴰ儈ࡲ䟿лˈ㥹⭈㟖ሩ䘉Ӌᵲ㥹Ⲵ䲔㥹᭸᷌൷∄ሩ➗㓴ᴹᡰ߿ᕡDŽᰐ䇪ᱟ൘ ࡲ䟿૽ᓄ⹄ウ઼ᑨ㿴Ⲵ䲔㥹ࡲ㦟᭸઼ᣇᙗ⹄ウѝˈ䙊ᑨ䜭нՊሩᵲ㥹ѝⲴ䲔㥹ࡲ ↻⮉䟿䘋㹼Ự⍻ˈަѝа䜘࠶ഐᱟ᱃ᝏᵲ㥹Պྲ亴ᵏ㻛䲔㥹ࡲᵰ↫ˈ㘼䲔㥹ࡲ 㦟᭸઼Ἵ⢙䮯࣯ࡉᱟєњᴤᑨ㿱Ⲵ⍻䟿ᤷḷDŽഐ↔ˈ㥹⭈㟖൘ᵲ㥹ѝ↻⮉䟿ⲴỰ ⍻ᮠᦞ䶎ᑨᴹ䲀DŽ Ѫ⺞ᇊ⢩ᇊ䲔㥹ࡲ൘ḀἽ⢙ѝⲴ↻⮉䟿ˈỰ⍻ᯩ⌅ᗵ享㓿䗷傼䇱ˈ⎸䲔 н਼ส䍘ӗ⭏Ⲵ䈟ᐞˈԕ㧧ᗇਟ䶐ⲴỰ⍻㔃᷌DŽ❦㘼ˈสҾᡁԜӾк䘠ഭ䱵䲔 㥹ࡲᣇᙗᵲ㥹䈳ḕ㖁ㄉѝ㧧ᗇⲴؑˈ䪸ሩ䘉 17 㻛ᣕѪާᴹ㥹⭈㟖ᣇᙗⲴ ᵲ㥹ˈ勌ᴹ⢩࡛䪸ሩḀᵲ㥹㘼䇮䇑Ⲵ䲔㥹ࡲ↻⮉Ự⍻ᯩ⌅DŽᵲ㥹、ᆖ亶ฏⲴ ⹄ウ㘵ਟ㜭Պሩᣇᙗᵲ㥹ѝ䲔㥹ࡲⲴ↻⮉䟿ᝏޤ䏓ᒦ䘋㹼⍻䈅ˈնሩҾ䘋ਓⲴ ˈⲴ䇱ᦞޣᙗޘབྷᇇߌӗ૱㘼䀰ˈ䘉㊫ᮠᦞᰐ⌅ᨀᴤཊо䖜สഐ⢙伏⭘ᆹ 䘉ҏᱟ㥹⭈㟖൘ᵲ㥹ѝ↻⮉䟿ᮠᦞ䶎ᑨቁⲴഐѻаDŽ 㲭❦㥹⭈㟖ᱟ䶎䘹ᤙᙗ䲔㥹ࡲˈնྲࡽᡰ䘠ˈ䮯ᵏ৽༽֯⭘অа䲔㥹ࡲᴰ㓸 ∄˄䜭Պሬ㠤ᣇᙗᵲ㥹Ⲵӗ⭏DŽᖃߌ≁䇶ࡠ㔗㔝֯⭘ḷㆮࡲ䟿௧ᯭḀ䲔㥹ࡲ ྲ㥹⭈㟖˅൘㠚ᇦߌ⭠Ⲵ䲔㥹᭸᷌ᐢнྲԕᖰᰦˈԆԜᗵ享䘹ᤙަԆᯩᔿ৫䲔䘉 Ӌ亭പⲴᵲ㥹ˈवᤜ֯⭘ަԆ㊫රⲴ䲔㥹ࡲᡆᱟ֯⭘⢙⨶䲔㥹ᯩᔿˈ∄ྲ㘫㙅ᡆ 䬴㥹DŽ㥹⭈㟖ሩߌ⭠ѝަԆⲴ᱃ᝏᵲ㥹ӽ❦ᴹ᭸ˈնণ֯ߌ≁൘䘹ᤙަԆ䲔㥹ᯩ ˈᔿⲴ਼ᰦ㔗㔝֯⭘㥹⭈㟖ˈԆԜҏнՊ䙊䗷ᨀ儈௧ᯭࡲ䟿ᶕ䈅മᵰ↫ᣇᙗᵲ㥹 ↔䇱ᣇᙗᵲ㥹аᇊՊ㻛ᵰ↫DŽ؍⌅ҏᰐˈ؍⧟ഐѪᨀ儈ࡲ䟿Ⲵᯩ⌅ᰒн㓿⍾ҏн ተѕṬ㇑⨶ᡰᴹߌ㦟Ⲵ֯⭘ḷㆮˈᤷᇊ⇿аߌ㦟ӗ૱൘ⴞⲴ⭘䙄؍⧟ཆˈ㖾ഭ ⌅кⲴ֯⭘ᯩ⌅ˈवᤜᴰվ઼ᴰ儈֯⭘ࡲ䟿DŽԫօнㅖਸḷㆮ㿴ᇊⲴ֯⭘൷Ѫ䘍 㹼ѪDŽഐ↔ˈ൘ਸ⌅֯⭘Ⲵࡽᨀлˈਟ亴ᵏ⢙᭦㧧㊭㋂ᡆᱟᣇᙗᵲ㥹ѝⲴ䲔㥹 ࡲ↻⮉䟿ᒦнՊഐѪߌ⭠ѝᵲ㥹ᱟާᴹᣇᙗ㘼ਈॆDŽ MON 87427 ᱟᆏኡ䜭ޜਨ᧘ࠪⲴ㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣૱ˈަ䳴ᙗಘ ᇈᒦн㙀ਇ㥹⭈㟖ˈഐ↔௧ᯭਜ਼ᴹ㥹⭈㟖Ⲵ䲔㥹ࡲਾਟ䗮ࡠᘛ䙏ᖫᓅⲴ৫䳴᭸᷌DŽ ަӗ૱ⴞⲴᱟѪ⇽ᵜ⭘Ҿࡦˈ㘼䶎⭠䰤Ἵˈഐ↔ᒦнՊ໎࣐ߌ⭠ѝ⧠ᴹ㥹 ⭈㟖ᣇᙗᵲ㥹Ⲵ䘹ᤙ࣋DŽ਼ṧˈ൘ԕ MON 87427 Ѫ⇽ᵜᗇࡠⲴᵲӔਾԓ⭏ ⢙ተᢩ߶Ⲵ㥹⭈㟖ḷㆮ֯⭘ࡲ䟿䘋㹼؍⧟䮯Ⲵߌ⭠ѝˈ㖾ഭߌ≁ᗵ享᤹➗㖾ഭ 㹼䰤/䎺亦௧ᯭ䲔㥹ˈഐ↔ˈ⦹㊣㊭㋂৺ަ࣐ᐕ㓴࠶ѝⲴ㥹⭈㟖↻⮉䟿亴ᵏнՊ ഐᆈ൘ᣇᙗᵲ㥹㘼ਁ⭏ਈॆˈߌ⭠ѝԫօਟ㜭ᆈ൘Ⲵ㥹⭈㟖ᣇᙗᵲ㥹ѝⲴ↻⮉䟿 ҏᱟྲ↔DŽ ᆏኡ䜭ޜਨ࡙⭘㓿傼䇱ᴹ᭸Ⲵ⍻䈅ᯩ⌅Ự⍻Ҷਜ਼ᴹ MON 87427 ᙗ⣦Ⲵ⦹㊣ ㊭㋂ѝ㥹⭈㟖Ⲵ↻⮉≤ᒣDŽѪᗇࡠ⦹㊣⋩઼⦹㊣㊹ѝ㥹⭈㟖↻⮉≤ᒣሩҾ㊭㋂ Ⲵ⎃㕙㌫ᮠ˄CF˅ˈ⢩࡛ሶᇎ傼ᶑԦѝⲴ௧ᯭࡲ䟿࣐བྷ˄~2 ؽ˅ˈণ֯൘䘉
IV ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ࣐བྷࡲ䟿ᶑԦлˈਜ਼ᴹ MON 87427 ᙗ⣦Ⲵ⦹㊣㊭㋂ѝ㥹⭈㟖Ⲵ↻⮉䟿Ѫ 0.91 ppmˈ䘌վҾ CODEX ൘⦹㊣㊭㋂к䇮ᇊⲴ㥹⭈㟖↻⮉䲀䟿ḷ߶˄5 ppm˅˄Codex Alimentarius, 2016˅ˈ䈧㿱ᢰᵟᣕ 16bDŽഐ↔ˈণ֯ᴹњ࡛ߌ≁䈅മ䙊䗷ᨀ儈 ࡲ䟿ᶕᵰ↫ᣇᙗᵲ㥹ˈ亴ᵏ⦹㊣㊭㋂ᡆᵲ㥹ѝⲴ㥹⭈㟖↻⮉≤ᒣҏнՊ䎵䗷 ٬DŽ CODEX 䇮ᇊⲴ MRL ࡽᡰ䘠ˈሩҾԫօа䲔㥹ࡲ㘼䀰ˈ৽༽䮯ᵏঅа֯⭘㘼н䖵ԕަԆᵲ㥹ྲ ᧗ࡦ⇥ᴰ㓸䜭Պሬ㠤ᣇᙗᵲ㥹㗔փⲴӗ⭏DŽഐ↔ˈᣇཊ䲔㥹ࡲⲴ༽ਸᙗ⣦䖜 สഐ૱ҏᱟаᴹ᭸䱢→ᣇᙗᵲ㥹ӗ⭏Ⲵ䖳ྭ䘹ᤙˈⴞࡽᐢᴹ䎺ᶕ䎺ཊⲴ䘉㊫ ӗ૱䰞цDŽ㘼 MON 87427 ਚᱟᆏኡ䜭ޜਨ᧘ࠪⲴ㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣૱ˈ ަ䳴ᙗಘᇈᒦн㙀ਇ㥹⭈㟖ˈഐ↔௧ᯭਜ਼ᴹ㥹⭈㟖Ⲵ䲔㥹ࡲਾਟ䗮ࡠᘛ䙏ᖫᓅⲴ ৫䳴᭸᷌DŽ㲭❦ MON 87427 ⦹㊣ҏ㜭ཏ㺘䗮 CP4 EPSPS 㳻ⲭˈնަӗ૱ⴞⲴᒦ 䶎ѪҶ൘୶ъॆἽᰦ䙊䗷௧ᯭ㥹⭈㟖᧗ࡦߌ⭠ᵲ㥹ˈ㘼ਚᱟѪ⇽ᵜ⭘Ҿࡦˈ ഐ↔ᒦнՊ໎࣐ߌ⭠ѝ⧠ᴹ㥹⭈㟖ᣇᙗᵲ㥹Ⲵ䘹ᤙ࣋DŽ
V ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߌъ䜘ޣҾMON 87427ᆹޘ䇴ԧ⭣䈧Ⲵഎ༽࠭ ˄2016ᒤ12ᴸ31ᰕ˅
VI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ҿߌъ䜘ᢩ༽ؑ࠭Ⲵ䈤᰾ޣਨޜᆏኡ䜭
䰞仈 1˖ ᵚᨀ㖾ഭߌ⭠ᵲ㥹ሩ㥹⭈㟖䲔㥹ࡲ㙀ਇᙗ⧠⣦᧿䘠઼࠶᷀Ⲵ䈖㓶 䍴ᯉ˗
䈤᰾˖㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣ MON 87427 ѝᕅޕҶⓀ㠚Ҿ൏༔ߌᵶ㧼 CP4 㧼 ṚⲴ cp4 epsps 㕆⸱ᒿࡇˈަ㺘䗮ӗ⢙ 5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦 ˄CP4 EPSPS˅ሩ㥹⭈㟖ⲴӢ઼ᙗ䶎ᑨվˈഐ↔㜭ཏ㙀ਇ㥹⭈㟖㊫䲔㥹ࡲDŽ
㥹⭈㟖㊫䲔㥹ࡲᐢ㓿୶ъॆᓄ⭘Ҷ䎵䗷40ᒤˈ⢩࡛ᱟ㠚кц㓚90ᒤԓԕᶕˈ 䲿⵰ᣇ㥹⭈㟖⢙୶ъॆἽⲴнᯝᢙབྷˈ㥹⭈㟖ᐢ㓿ᡀѪߌ≁ᴰѫ㾱ǃᴰᴹ᭸ Ⲵߌ⭠ᵲ㥹᧗ࡦ⇥ѻаDŽդ䲿⵰㥹⭈㟖Ⲵ䮯ᵏᓄ⭘ˈṩᦞHeap(2016a,b,c)Ⲵ ᣕ䚃ˈवᤜ㖾ഭ൘Ⲵཊњഭᇦҏࠪ⧠Ҷ㥹⭈㟖ᣇᙗᵲ㥹Ⲵᣕˈᴹ17㥹⭈㟖 ᣇᙗᵲ㥹൘㖾ഭⲴ38њᐎᴹᣕDŽ
㲭❦ԫօа䲔㥹ࡲ䮯ᵏᓄ⭘ᴰ㓸䜭Պሬ㠤ᵲ㥹ᣇᙗӗ⭏ˈնᱟᵲ㥹ᣇᙗӗ ⭏ҏᱟਟԕ䙊䗷аӋ᧚ᯭᔦ㕃ᒦ᧗ࡦⲴˈ⢩࡛ᱟᖃ䘉Ӌ᧚ᯭоާᴹн਼⭘ᯩᔿ Ⲵཊн਼䲔㥹ࡲ༽ਸ֯⭘ᰦˈᴤ㜭བྷབྷ䱽վᣇᙗἽṚᆈ⍫৺䘋а↕㑱㹽Ⲵਟ㜭DŽ ˖ؑޣԕлѪᵲ㥹ᣇᙗⲴаӋ
ᵲ㥹ᱟᖡ૽⢙ӗ䟿ᴰ䟽㾱Ⲵഐ㍐ѻаˈਟሬ㠤ѕ䟽Ⲵӗ䟿ᦏཡ઼㓿⍾ᆖᖡ ૽˄Ross and Lembi, 1985; Aref and Pike, 1998; Dalley et al., 2001˅DŽഐ↔ˈᵲ㥹 䱢䲔ᱟߌъ⭏ӗѝ䶎ᑨ䟽㾱Ⲵа䜘࠶DŽⴞࡽᑨ⭘Ⲵ䲔㥹ᯩ⌅ѫ㾱ᴹഋ˖㙅᧚ ᯭǃ⢙⨶䲔㥹ǃॆᆖ䲔㥹઼⭏⢙䲔㥹˄Knezevic and Cassman, 2003, HRAC, 2016˅˖
ᓧ≤ᒣ઼ᣁࡦᵲ㥹⭏䮯㘼䟷ਆⲴњ㌫ࡇ㙅ڕ⢙㙅᧚ᯭ䲔㥹ᱟѪᨀ儈 - ᯩ⌅ᙫ઼ˈवᤜ䘲ᖃ♼Ⓓǃᯭ㛕ǃ䘹ᤙ䘲ᓄᓖྭⲴ૱ǃ䘲ᇌⲴ䰤 ⢙ᣔㅹüüަⴞⲴ䜭ᱟѪҶᑞࣙ؍⢙䐍઼ᇶᓖǃ䟷⭘䖞઼㾶൏ 䮯࣯䎵䗷ᵲ㥹ᡆᣁࡦᵲ㥹⭏䮯DŽ
䲔㥹वᤜ֯⭘⢙⨶ᯩ⌅ᒢᢠᵲ㥹⭏䮯ˈवᤜᵪỠ㘫㙅ǃ࠸ࢢˈᡆࣘ⨶⢙ - 䲔㥹DŽ
ᆖ䲔㥹वᤜ֯⭘䲔㥹ࡲ᧗ࡦᵲ㥹DŽॆ -
⭏⢙䲔㥹वᤜ֯⭘ᰶ㲛ǃᗞ⭏⢙઼伏㥹ࣘ⢙ᶕ᧗ࡦᵲ㥹DŽн䗷བྷ䉶ǃ⦹㊣ - ỹ㣡ㅹаᒤ⭏⢙Ⲵ⭏ӗѝᖸቁ֯⭘⭏⢙䲔㥹᧚ᯭDŽ઼
ᡰᴹഋᯩ⌅䜭㜭ཏӾаᇊ〻ᓖкᴹ᭸䱢⋫ᵲ㥹ˈ൘ᇎ䱵ѝ䙊ᑨ㓴ਸ ⭘DŽֻྲˈ䲔㥹ࡲᑨᑨо㙅᧚ᯭа䎧֯⭘ˈ䇨ཊᛵߥлˈ㘫㙅઼⭏⢙᧚ᯭҏ֯ ˈ⌅㻛䇔Ѫᱟ֯⭘䲔㥹ࡲㅹѫ㾱䲔㥹᧚ᯭⲴ䖵ࣙ⇥˄Beckie, 2006˅DŽॆᆖᯩ ࡛ᱟ䲔㥹ࡲо㙀䲔㥹ࡲ⢙䝽ਸ֯⭘ˈਟѪߌ≁ᨀᴹ᭸Ⲵᵲ㥹᧗ࡦ⇥ˈ䘹⢩ ᤙᙗൠ৫䲔ᵲ㥹ˈ㘼䇙⢙⭏䮯ᴤ࣐ᰪⴋ˄Knezevic and Cassman, 2003˅DŽᦞՠ ㇇ˈ൘௧ᯭ䲔㥹ࡲⲴᶑԦлˈ⭡ᵲ㥹ᕅ䎧Ⲵӗ䟿ᦏཡӾ1%㠣20%нㅹˈ㘼ྲ᷌
VII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 н௧ᯭ䲔㥹ࡲˈӗ䟿ᦏཡࡉՊ໎࣐㠣10㠣75%˄Dalley et al., 2001, Dille et al., 2015, Dille et al., 2016˅DŽ
⭘䲔㥹ࡲሩᵲ㥹䘋㹼᧗ࡦ㜭ཏᴹ᭸Ⲵ߿ቁӗ䟿ᦏཡˈѪᒯབྷߌ≁ᑖᶕҶ֯ ਟ㿲Ⲵ᭦⳺DŽ֯⭘䲔㥹ࡲሩᵲ㥹䘋㹼᧗ࡦҏ߿ቁҶ⭘Ҿ㘫㙅ⲴӪᐕǃ⟳ᯉ઼ᵪỠ ,ᤱර㙅ൠ४ᒯ⌋䟷⭘˄Fernandez-Cornejo and McBride؍䇮༷ˈഐ↔㻛≤൏ ᤱර؍Gianessi, 2005; Gianessi and Reigner, 2006˅DŽྲ᷌⋑ᴹ䲔㥹ࡲˈ≤൏ ;2002 ,ᤱⲴⴞḷ˄CTIC؍㲰઼໎ᕪ≤൏ץ㙅ሶᰐ⌅ᇎ⧠ˈҏቡᰐ⌅ᇎ⧠߿ቁ൏༔ 2016˅DŽ䲔㥹ࡲᱟਟᤱ㔝ߌъѝнਟᡆ㕪Ⲵ䟽㾱㓴ᡀ䜘࠶˄Knezevic and Cassman, 2003˅DŽᵲ㥹㇑⨶઼ᣇᙗᵲ㥹Ⲵ⋫⨶ҏᱟਟᤱ㔝ߌъᇎ䐥ѝ䶎ᑨ䟽㾱Ⲵ㓴ᡀ䜘࠶DŽ ᵲ㥹ᣇᙗਟᇊѹѪḀ䲔㥹ࡲⴞḷᵲ㥹ѝⲴḀа㗔փ㧧ᗇҶ㙀ਇ䘉䲔㥹 ࡲⲴ㜭࣋ˈ௧ᯭ䈕䲔㥹ࡲሩ⢩ᇊ㗔փᵲ㥹нᴹ᭸˄WSSA, 2011˗HRAC, 2016˅DŽ ᵲ㥹Ⲵ䲔㥹ࡲᣇᙗᒦ䶎ᯠޤ䰞仈ˈᆳᱟа㠚❦ਁ⭏Ⲵ䗷〻DŽἽ⢙㗔փਟ㜭Պᆈ ൘ḀӋቁᮠњփˈഐㅹսสഐᐞᔲ㘼ሩ㠤↫ර䲔㥹ࡲާᴹཙ❦㙀ਇᙗˈสҾ㠚❦ 䘹ᤙ⨶䇪ˈ௧ᯭ䲔㥹ࡲਾˈᝏἽṚ㗔փ㻛䲔㥹ࡲᵰ↫ˈӵᴹ䘉Ӌቁᮠ㙀ਇරњ փᆈ⍫лᶕDŽྲ᷌৽༽֯⭘਼а䲔㥹ࡲ㘼н࣐ԕަԆᵲ㥹᧗ࡦ᧚ᯭ˄ྲ֯⭘ ˈ˅⭘ᵪࡦн਼ⲴަԆॆᆖᯩᔿ䲔㥹ˈᡆ֯⭘ᵪỠ䲔㥹ǃ㘫㙅䲔㥹ǃᡆ⭏⢙䲔㥹 ԕᰦᰕˈ䘉Ӌཙ❦ᩪᑖᴹᣇᙗสഐⲴቁᮠњփՊ䙀⑀ਁኅᡀѪѫሬ㗔փٷ ˄Beckie and Reboud, 2009; WSSA, 2011; HRAC, 2016˅DŽሩԫօа䲔㥹ࡲᶕ䈤ˈ ৽༽䮯ᵏঅа֯⭘䜭ᴹਟ㜭ӗ⭏ᣇᙗᵲ㥹㗔փˈަᣇᙗ╄ॆⲴᵪࡦᱟањ༽ᵲⲴ 䗷〻ˈн䟷⭘ަԆᵲ㥹⋫⨶ᶑԦлˈ⭡ᣇᙗㅹսสഐਁ⭏仁⦷ǃᣇᙗᵪࡦǃᱮᙗ /䳀ᙗᣇᙗㅹսสഐǃᣇᙗሿⲴሩ䘲ਸᓖԕ৺䲔㥹ࡲⲴ௧ᯭ仁⦷઼ᤱ㔝ᴹ᭸ ᰦ䮯ㅹഐ㍐㔬ਸߣᇊ˄Jasieniuk et al., 1996; Beckie and Reboud, 2009; Sammons et al., 2007˅DŽሩн਼䲔㥹ࡲᶕ䈤ˈަӗ⭏ᣇᙗᵲ㥹Ⲵਟ㜭ᙗҏнቭ਼ˈḀӋ䲔 㥹ࡲ˄ྲALS઼ACCase㊫˅оަԆ䲔㥹ࡲ˄ྲ⭏䮯㍐ǃ㥹⭈㟖ǃ㥹䬥㟖઼҉䞠 㤟㜪㊫˅∄ˈࠪ⧠ᣇᙗᵲ㥹Ⲵ䙏ᓖᴤᘛDŽ䲔㥹ࡲᣇᙗⲴ䰞仈ҏнᱟ㖾ഭᡰ⢩ᴹ Ⲵ⧠䊑ˈޘ⨳㤳തሩḀӋ䲔㥹ࡲᴹ㙀ਇᙗⲴᵲ㥹઼ᣇᙗᵲ㥹㓖ᴹ250ཊˈ࠶ ᐳҾ66њഭᇦ˄Heap, 2016a; b˅DŽлമᡰ⽪Ѫޘ⨳㤳ത⺞䇔Ⲵн਼䲔㥹ࡲᇦ᯿ ᣇᙗᵲ㥹㊫ᮠⴞਈॆⲴᴢ㓯ˈн਼仌㢢ԓ㺘н਼Ⲵ䲔㥹ࡲᇦ᯿ˈᯌ⦷ԓ㺘ᣇᙗ ᵲ㥹ӗ⭏Ⲵ䙏ᓖDŽӾлമਟԕⴻࠪˈн਼䲔㥹ࡲᇦ᯿ᣇᙗᵲ㥹ӗ⭏Ⲵ䙏ᓖн਼DŽ 䘁ᒤᶕˈჂփᶌࠪањᯠ䇽Ā䎵㓗ᵲ㥹āˈ䘉њ䇽ަᇎᒦ⋑ᴹ、ᆖⲴᇊ ѹˈնᆳᑨᑨ㻛⭘ᶕᤷԓ䛓Ӌ⭡Ҿ৽༽֯⭘Ḁ䲔㥹ࡲˈ൘ᤱ㔝࣋лӗ⭏ᣇᙗ Ⲵᵲ㥹ˈ∄ྲᣇᙗ⭏⢙රᡀѪߌ⭠ѝᑨ㿱ᵲ㥹Ⲵ⧠䊑˄WSSAˈ2016˅DŽഐ↔ˈ Āᣇᙗᵲ㥹āDŽྲࡽᡰ䘠ˈ䲔㥹ࡲᣇᙗ䰞仈ᒦڊᡰ䉃Ā䎵㓗ᵲ㥹āᇎ䱵кᓄ䈕ਛ 䶎ᯠޤһ⢙ˈҏᒦ䶎Ḁᵲ㥹⢩ᴹⲴㄎҹᙗ⢩ᖱDŽ㲭❦൘䗷৫Ⲵॱࠐᒤѝˈ⭡Ҿ 䎺ᶕ䎺ཊⲴߌ≁ӵӵ䎆Ҿঅа⭘ᵪࡦⲴᵲ㥹⋫⨶⇥ˈᡁԜⴻࡠ䎺ᶕ䎺ཊⲴ ߌ⭠ᔰࠪ⧠ᣇᙗᵲ㥹䰞仈ˈն䘉ᣇᙗᵲ㥹䰞仈ᒦ䶎ӵ䲀Ҿቁᮠ䲔㥹ࡲˈᇎ䱵 кˈ൘䗷৫40ᒤѝˈᖸཊ䲔㥹ࡲ䜭ᴹᓄⲴᣇᙗᵲ㥹㻛ᣕ䚃˄ྲлമᡰ⽪˅ˈḀ Ӌᵲ㥹⭊㠣䘋ॆࠪሩєᡆєԕк䲔㥹ࡲⲴᣇᙗ˄WSSAˈ2016˅DŽ
VIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 н਼䲔㥹ࡲӗ⭏ᣇᙗᵲ㥹Ⲵᮠ䟿䲿ᰦ䰤ਈॆᴢ㓯 ྲ᷌н㜭⭘ަԆ㊫රⲴ䲔㥹ࡲᡆ䟷⭘ަԆ⇥ᶕ᧗ࡦᣇᙗᵲ㥹㗔փˈ䲔㥹ࡲ ᣇᙗᵲ㥹ᖸᴹਟ㜭ՊᡀѪᖡ૽ߌъ⭏ӗѝⲴ䲀ࡦᙗഐ㍐˄Norsworthy et al., 2012˅ˈ նⴞࡽ䘈⋑ᴹԫօа䲔㥹ࡲࠪ⧠Ҷ䘉Ѹѕ䟽Ⲵ䰞仈DŽ↔ཆˈ⹄ਁ㘵ǃ᭯ᓌ઼ ޣ、⹄ᵪᶴҏޡ਼ࣚ࣋ˈࡦᇊࠪҶаӋ㢟ྭⲴᵲ㥹䱢⋫᧚ᯭᶕᑞࣙᔦ㕃ᵲ㥹ᣇᙗ Ⲵӗ⭏ˈ䜘䰘ҏ൘〟ᶱ᧘㹼䘉Ӌᵲ㥹䱢⋫᧚ᯭ˄Norsworthy et al., 2012; WSSA, HRAC, 2016˅DŽᴰ֣Ⲵᵲ㥹䱢⋫᧚ᯭवᤜ⢙䖞ǃ䘲ᖃ♼Ⓓǃ䘲ᇌ㹼䐍 ;2015 ઼ᇶᓖㅹ㙅᧚ᯭˈԕ৺㘫㙅ǃ⭠䰤㿲ሏ઼ࣘ䲔㥹ㅹᵪỠ᧚ᯭDŽ䶒ሩ∄䖳 亭പⲴᵲ㥹ᰦˈ䘉Ӌᵲ㥹䱢⋫᧚ᯭ䘈䴰㾱оᴹ᭸ᡀ࠶н਼Ⲵཊ㊫䲔㥹ࡲ㔃ਸ֯⭘DŽ н਼⭘ᵪࡦⲴ䲔㥹ࡲԕ᧘㦀ࡲ䟿਼ᰦǃ亪ᒿǃᡆ䳄ᒤ֯⭘ˈ䘉Ӌ㓴ਸਟᶱབྷൠ ߿ቁᣇᙗᵲ㥹ӗ⭏઼⭏Ⲵਟ㜭ᙗ˄Norsworthy et al., 2012˅DŽ 㲭❦Ḁ䲔㥹ࡲⲴ֯⭘ᴰ㓸䜭ᴹਟ㜭Պӗ⭏ᣇᙗᵲ㥹ˈն䘉ᣇᙗⲴࠪ⧠㜭 ཏ䙊䗷㢟ྭⲴ䱢⋫᧚ᯭǃ⹄ウ઼ᮉ㛢㘼㻛ᔦ㕃ǃ᧗ࡦ઼㇑⨶DŽ䲔㥹ࡲӽᱟⴞࡽᴰ ѫ㾱Ⲵᵲ㥹䱢⋫⇥ˈ֯⭘⭘ᵪࡦн਼㘼䲔㥹䉡ᴹ䟽ਐⲴ䲔㥹ࡲᴹࣙҾ߿㕃ᵲ 㥹ᣇᙗⲴӗ⭏˄Beckie, 2006; HRAC, 2016˅DŽ∄ྲˈྲ᷌⢙ਟ਼ᰦ㙀ਇཊ䲔 㥹ࡲˈণ֯⭠䰤ᵲ㥹ሩަѝḀа㊫䲔㥹ࡲӗ⭏Ҷᣇˈ䈕ᵲ㥹ҏਟԕ㻛ަԆ㊫䲔㥹 ࡲᵰ↫DŽഐ↔ˈᣇཊн਼⭘ᵪࡦ䲔㥹ࡲⲴ⢙ᴹࣙҾ൘ߌ≁Ⲵᵲ㥹䱢⋫փ㌫ ѝᕅޕᯠⲴ䲔㥹ࡲˈӾ㘼ᴹਟ㜭߿㕃ᵲ㥹ᣇᙗⲴ䘋а↕ਁኅDŽ
IX ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⴞࡽᐢ㓿ᴹаӋሩཊ䲔㥹ࡲާᴹᣇᙗⲴӗ૱᧘ੁᐲ൪ˈѪߌ≁ᨀᴤཊᵲ 㥹䱢⋫ᯩ⌅ˈԕਟᤱ㔝ਁኅⲴᯩᔿᕪॆᵲ㥹㇑⨶᧚ᯭ˄USDA-APHIS, 2014; 2015a; ᤱ⧠ᴹ؍b and 2016˅DŽᵲ㥹㇑⨶㌫㔏ᡀ࣏о䎆Ҿਟ⭘䲔㥹ᯩᔿⲴཊṧᙗDŽ 䲔㥹ࡲ˄ྲ㥹⭈㟖઼㥹䬥㟖˅Ⲵᴹ᭸ᙗˈ਼ᰦнᯝᕅޕᢰᵟࡋᯠӗ૱˄ྲᣇ哖㥹 ߌ≁ᤕᴹ䏣ཏᕪབྷⲴᐕާᶕ⋫⨶ᵲ㥹ᒦ䙊䗷ཊṧॆⲴᵲ㥹䱢⋫؍⺞⭿⢙˅ਟ ⇥ᔦ㕃ᵲ㥹ᣇᙗⲴӗ⭏DŽ
৲㘳᮷⥞: 1. Aref, S. and D.R. Pike. 1998. Midwest farmers’ perceptions of crop pest infestations. Agronomy Journal 90:819-825. 2. Beckie, H.J. 2006. Herbicide-resistant weeds: Management tactics and practices. Weed Technology 20: 793-814. 3. Beckie, H.J., and X. Reboud. 2009. Selecting for weed resistance: Herbicide rotation and mixture. Weed Technology 23:363-370. 4. CTIC. 2016. Top 10 conservation tillage benefits: Conservation for agriculture's future. 5. Conservation Technology Information Center, West Lafayette, Indiana. 6. http://www.ctic.purdue.edu/resourcedisplay/293/ [Accessed September, 2016]. 7. Dalley, C.D., K.A. Renner, and J.J. Kells. 2001. Weed competition in Roundup Ready soybean and corn. Michigan Soybean Promotion Committee, Corn Marketing Program of Michigan, East Lansing, Michigan. 8. Dille, J.A., Sikkema, P.H., Everman, W.J., Davis, V.M. and I.C. Burke. 2015. Perspecitves on corn yield losses due to weeds in North America. Weed Science Society of America., Champaign, Illinois. http://wssa.net/wp-content/uploads/WSSA-2015-Corn-Yield-Loss-poster-updated-calc.p df [Accessed September, 2016] 9. Dille, J.A., Sikkema, P.H., Everman, W.J., Davis, V.M. and I.C. Burke. 2016. Perspecitves on soybean yield losses due to weeds in North America. Weed Science Society of America., Champaign, Illinois. http://wssa.net/wp-content/uploads/WSSA-2016-Soybean-Yield-Loss-poster.pdf [Accessed September, 2016] 10. Fernandez-Cornejo, J. and W.D. McBride. 2002. Adoption of bioengineered crops. U.S. Department of Agriculture, Washington, D.C. http://www.ers.usda.gov/publications/aer810/ [Accessed September, 2016] 11. Gianessi, L.P. 2005. Economic and herbicide use impacts of glyphosate-resistant crops. Pest Management Science 61: 241-245. 12. Gianessi, L. and N. Reigner. 2006. The value of herbicides in U.S. crop production: 2005 update. CropLife Foundation, Washington, D.C. 13. Heap, I. 2014. Global perspectives of herbicide-resistant weeds. Pest Management Science 70: 1306-1315. 14. Heap, I. 2016a.The International Survey of Herbicide Resistant Weeds: herbicide resistant weeds by species and site of action. WeedScience.org, Corvallis, Oregon. http://www.weedscience.org/Summary/SpeciesbySOATable.aspx [Accessed September, 2016]. 15. Heap, I. 2016b.The International Survey of Herbicide Resistant Weeds: Herbicide resistant weeds by country and site of action. WeedScience.org, Corvallis, Oregon. http://www.weedscience.org/Summary/CountrySummary.aspx [Accessed September, 2016]. 16. Heap, I. 2016c. The International Survey of Herbicide Resistant Weeds: Weeds Resistant to EPSP synthase inhibitors. WeedScience.org, Corvallis, Oregon.
X ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 http://www.weedscience.org/Summary/MOA.aspx [Accessed February, 2017]. 17. HRAC. 2016. Guideline to the management of herbicide resistance. Herbicide Resistance Action Committee. [Accessed September, 2016] http://hrac.tsstaging.com/files/Management-of-Herbicide-Resistance.pdf 18. Jasieniuk, M., A.L. Brûlé-Babel, and I.N. Morrison. 1996. The evolution and genetics of herbicide resistance in weeds. Weed Science 44:176-193. 19. Knezevic, S.Z. and K.G. Cassman. 2003. Use of herbicide-tolerant crops as a component of an integrated weed management program. Crop Management Online, St. Paul, Minnesota. 20. Norsworthy, J.K., S.M. Ward, D.R. Shaw, R.S. Llewellyn, R.L. Nichols, T.M. Webster, K.W. Bradley, G. Frisvold, S. B. Powles, N. R. Burgos, W.W. Witt, and M. Barrett. 2012. Reducing the risks of herbicide resistance: Best management practices and recommendations. Weed Science: Special Issue: 31-62. 21. Ross, M.A. and C.A. Lembi. 1985. Applied Weed Science. Burgess Publishing Co., Minneapolis. 22. Sammons, R.D., D.C. Heering, N. DiNicola, H. Glick, and G.A. Elmore. 2007. Sustainability and stewardship of glyphosate and glyphosate-resistant crops. Weed Technology 21:347-354. 23. USDA-APHIS. 2014. Determination of Nonregulated Status for Dow AgroSciences’ DAS-40278-9 Corn. U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Washington, D.C. 24. USDA-APHIS. 2015a. Determination of Nonregulated Status for Monsanto Company MON 87708 Soybean. U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Washington, D.C. 25. USDA-APHIS. 2015b. Determination of Nonregulated Status for Monsanto Company MON 88701 Cotton. U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Washington, D.C. 26. USDA-APHIS. 2016. Determination of Nonregulated Status for Monsanto MON 87419 Maize. U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Washington, D.C. 27. WSSA. 2011. Herbicide Resitance Management Lesson 3. Weed Science Society of America., Champaign, Illinois. http://wssa.net/wp-content/uploads/resistancemodules/three/index.htm 28. WSSA. 2015. WSSA lesson module: Herbicide resistant weeds. Weed Science Society of America, Champaign, Illinois http://wssa.net/wpcontent/uploads/resistancemodules/five/index.htm 29. WSSA. 2016. WSSA Fact Sheet: Dispelling Common Misconceptions about Superweeds. Public Awareness Committee of the Weed Science Society of America., Champaign, Illinois. http://wssa.net/wp-content/uploads/WSSA-Weed-Science-Definitions.pdf
XI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䰞仈 2˖ ⦹㊣㣡㊹⍫࣋Ⲵ⍻ᇊᯩ⌅ᰐ⌅⺞ᇊ⦹㊣ᮓ㊹ਾн਼ᰦᵏⲴ㣡㊹⍫࣋ˈ ф㕪ቁާփⲴỰ⍻ᮠᦞ˗ 䈤᰾˖ MON 87427 ࡙⭘Ҷ⢩ᇊⲴࣘᆀ-ਜ਼ᆀ㓴ਸˈഐ↔䳴ᙗಘᇈн㜭㙀ਇ 㥹⭈㟖DŽ൘⦹㊣ᵲӔࡦ䗷〻ѝˈਟԕ⭘ਜ਼ᴹ MON 87427 䖜ॆփⲴ⦹㊣㠚Ӕ ㌫⇽ᵜˈُࣙҾ㥹⭈㟖䲔㥹ࡲⲴ௧ᯭӗ⭏䳴ᙗн㛢ˈݽ৫Ӫᐕᣭ䳴Ⲵ↕僔ˈབྷ བྷ㢲㓖ҶࡦᡀᵜDŽ ൘ӗ૱ᔰਁѻࡍˈᆏኡ䜭ޜਨሩ MON 87427 䘋㹼Ҷवᤜ㣡㊹⍫࣋൘Ⲵޘ 䶒⧟ຳᆹޘᙗ䇴ԧDŽ⧟ຳᆹޘ䇴ԧⲴ㔃᷌䇱ᇎˈоᑨ㿴⦹㊣∄ˈMON 87427 ⦹㊣䖜ਈѪᴹᇣ⭏⢙઼ᵲ㥹ॆⲴ▌࣯ᒦ⋑ᴹ໎࣐DŽަѝˈሩ MON 87427 ⦹㊣㣡 ㊹⍫࣋ⲴỰ⍻ᴰࡍ⭘ӊশኡབྷḃ㢢⌅䘋㹼ˈ䈖㓶ⲴỰ⍻ᯩ⌅৺㔃᷌㿱ᵜ⭣䈧ҖⲴ 72 亥DŽӊশኡབྷḃ㢢Ⲵ㔃᷌㺘᰾ MON 87427 㣡㊹Ⲵ⍫઼࣋ᖒᘱоᑨ㿴⦹㊣∄ 䜭⋑ᴹާᴹ⭏⢙ᆖѹⲴᐞᔲDŽ ṩᦞߌъ䜘৽侸㿱ˈᆏኡ䜭ޜਨҾ 2017 ᒤࡍ൘㖾ഭ༿ေཧἽҶਜ਼ᴹ MON 87427 䖜ॆփⲴ⦹㊣ᵲӔ઼нਜ਼䈕䖜ॆփⲴᑨ㿴ᵲӔሩ➗ˈ਼ᰦ䘋㹼 ⍻䈅Ⲵ䘈ᴹ 5 њ৲➗૱ˈ⭘Ҿᗇࡠ㣡㊹⍫࣋ਈॆⲴཙ❦ਈᔲ㤳തDŽ䈅傼䟷⭘ᆼ ޘ䲿ᵪ४㓴䇮䇑ˈ 4 њ䟽༽DŽ䈅傼⭠ሿ४ἽṚᔰᮓ㊹ਾˈ䘹ᤙ൘ѝॸѻࡽ 䟷䳶㣡㊹ṧ૱ˈ㣡㊹䟷䳶ᰦᓖᒣ൷Ѫ 21.7°CDŽሶ਼аሿ४н਼ἽṚк᭦䳶Ⲵ㣡 ㊹ਸਾ⭘Ҿ㣡㊹㨼ਁ䈅傼ˈሶ㣡㊹㖞Ҿ㨼ਁษޫสࡽҾᇔ˄~25°C˅ᆈ᭮ˈ ᇔᆈ᭮ᰦ䰤࠶࡛Ѫ 0ǃ2ǃ3.5 ઼ 5 ሿᰦDŽ 㣡㊹㨼ਁ䈅傼ᡰ⭘ษޫสѪՈॆⲴ㚊҉Ҽ䞷˄PEG˅ѪสⲴ⭏䮯ӻ䍘 ˄Subbaiah, 1984; Zhang et al., 1997; Tandon et al., 1999˅ˈަѝਜ਼ᴹ 10%㭇㌆ǃ 6% PEGǃ0.6%Ἵ⢙⩬㜲ǃ0.005%⺬䞨ǃ0.1%≟ॆ䫉઼ 0.001%⼧䞨䫮DŽሶ㣡㊹㖞 Ҿษޫ⳯ѻਾ൘ᇔ᭮㖞㠣ቁ 2 ሿᰦਾ䘋㹼㣡㊹㨼ਁ䇑ᮠDŽ ⭘ AmScope Simul-Focal йⴞᱮᗞ䮌˄60×᭮བྷ˅䘋㹼㣡㊹㨼ਁ䇑ᮠˈᖃ㣡 ㊹㇑䮯ᓖı㣡㊹ⴤᖴᰦ㿶Ѫ㨼ਁ˄Kakani et al., 2002˅DŽ⇿њᰦ䰤⛩Ӿ⇿ањሿ ४᭦䳶Ⲵ㣡㊹㠣ቁ䇑ᮠ 125 ㋂ˈ㨼ਁ⦷⭡㨼ਁⲴ㣡㊹ᮠⴞ䲔ԕ䇑ᮠⲴޘ䜘㣡㊹ᮠ ⴞਾ҈ԕ 100 ᗇࡠDŽᓄ⭘ SAS PROC MIXED (SAS, 2012)㔏䇑ᆖ䖟Ԧሩ㨼ਁ⦷ᮠ ᦞ䘋㹼 ANOVA ࠶᷀DŽ⭘ᱮ㪇ᙗ≤ᒣѪ 5%Ⲵ t-test ሩ⇿њᰦ䰤⛩ MON 87427 ઼ ᑨ㿴ሩ➗൷䘋㹼ᡀሩ∄䖳DŽ䈅傼㔃᷌㿱㺘 1 ઼㺘 2DŽ ⭡Ҿਇ䚇Ր઼⧟ຳㅹཊഐ㍐Ⲵᖡ૽˄Fonseca and Westgate, 2005; Nielsen, 2010˅ˈа㡜㘼䀰㣡㊹㨼ਁ䈅傼㔃᷌Ⲵਈᔲ㤳തᖸབྷˈ㨼ਁ⦷㤳തਟӾ 13%ࡠ 75%нㅹ˄Fonseca and Westgate, 2005˅DŽо᮷⥞ᣕ䚃а㠤ˈᵜ⹄ウѝн਼৲㘳 ૱н਼ᰦ䰤⛩Ⲵ㣡㊹㨼ਁ⦷Ⲵਈᔲ㤳തҏ䖳བྷ˄㺘 2˅DŽ㔏䇑ᆖ࠶᷀㔃᷌㺘᰾ˈ ൘⇿њᰦ䰤⛩ˈMON 87427 оᑨ㿴ሩ➗Ⲵ㨼ਁ⦷൷ᰐ㔏䇑ᆖᱮ㪇ᙗᐞᔲ˄㺘 1˅ˈ ф MON 87427 н਼ᰦ䰤⛩㣡㊹㨼ਁ⦷Ⲵ൷٬սҾ৲㘳૱Ⲵ㠚❦ਈᔲ㤳തDŽ ᵜ⹄ウⲴ㔃᷌оѻࡽӊশኡབྷ⌅㣡㊹ḃ㢢Ⲵ㔃᷌а㠤ˈ൷᭟ᤱ MON 87427 оᑨ㿴ሩ➗㣡㊹⍫࣋ᖃⲴ㔃䇪DŽ↔ཆˈ2008 ᒤ㖾ഭ⭠䰤䈅傼઼ 2014 ᒤ൘ਹ᷇ ⴱ䘋㹼Ⲵഭ⧟ຳᆹޘỰ⍻Ⲵ㔃᷌൷㺘᰾ MON 87427 ઼ᑨ㿴ሩ➗൘㺘ර৺ߌ㢪
XII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᙗ⣦઼о⧟ຳӂㅹᯩ䶒൷ᖃˈᵚ㿲ሏࡠԫօᴹ⭏⢙ᆖѹⲴᐞᔲDŽ㺘᰾оᑨ 㿴ሩ➗∄ˈMON 87427 䖜ਈѪᴹᇣ⭏⢙ᡆᵲ㥹Ⲵ▌࣯ᒦ⋑ᴹ໎࣐DŽ↔ཆˈਜ਼ ᴹ MON 87427 Ⲵ⦹㊣㊭㋂ӗ૱ӵݱ䇨Ѫ࣐ᐕᯉ䘋ਓࡠѝഭˈнՊ൘ѝഭຳ 䘋㹼Ἵˈഐ↔ MON 87427 ἽṚ৺ަᡰӗ㣡㊹Ⲵ⧟ຳ᳤䵢䟿ᶱվDŽ
˅SD±㺘 1. MON 87427 ઼ᑨ㿴ሩ➗㣡㊹⍫࣋Ⲯ࠶ᮠᙫ㔃㺘˄൷٬ T11 T22 T33 T44 ᶀᯉ ᶀᯉ㊫ර ˄%˅ ˄%˅ ˄%˅ ˄%˅ MON 87427 ⍻䈅⢙ 66.5 (3.6) 70.6 (4.4) 70.7 (2.8) 68.1 (3.1) LH198+LH287 ᑨ㿴ሩ➗ 57.5 (1.3) 63.6 (2.4) 60.6 (3.1) 59.6 (3.1) ৲㘳㤳ത 5 ৲㘳㤳ത 54.7 – 74.5 56.5 – 75.1 48.2 – 72.7 49.4 – 72.5 1 ᭦䳶ਾ・ণ䇑ᮠ˗2 ᭦䳶ਾᇔ᭮㖞 2 ሿᰦ˗3 ᭦䳶ਾᇔ᭮㖞 3.5 ሿᰦ˗4 ᭦䳶ਾᇔ ᭮㖞 5 ሿᰦ˗5 5 њ৲㘳૱Ⲵᴰབྷ٬઼ᴰሿ٬DŽ
˅SD±㺘 2. ৲㘳૱㣡㊹⍫࣋Ⲯ࠶ᮠᙫ㔃㺘˄൷٬ T11 T22 T33 T44 ᶀᯉ ᶀᯉ㊫ර ˄%˅ ˄%˅ ˄%˅ ˄%˅ Stewart S750 ৲㘳૱ 57.8 (3.2) 58.7 (3.3) 52.2 (4.1) 49.4 (3.1) Stine 9628 ৲㘳૱ 54.7 (5.9) 56.5 (8.0) 48.2 (6.1) 50.3 (7.1) DKC64-85 ৲㘳૱ 70.8 (5.1) 75.0 (3.4) 72.6 (5.5) 66.3 (3.8) Lewis 1613 ৲㘳૱ 74.5 (2.1) 68.1 (7.1) 57.4 (6.9) 56.6 (11.1) HCL654+LH244 ৲㘳૱ 69.2 (1.0) 75.1 (2.6) 72.7 (3.7) 72.5 (1.1) 1 ᭦䳶ਾ・ণ䇑ᮠ˗2 ᭦䳶ਾᇔ᭮㖞 2 ሿᰦ˗3 ᭦䳶ਾᇔ᭮㖞 3.5 ሿᰦ˗4 ᭦䳶ਾᇔ ᭮㖞 5 ሿᰦ˗5 5 њ৲㘳૱Ⲵᴰབྷ٬઼ᴰሿ٬DŽ
XIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䰞仈 3˖ 㕪ቁ䖜สഐ⦹㊣ሩ劬㊫ǃ㊫ᖡ૽Ⲵ∂⨶ᆖ䇴ԧ䍴ᯉDŽ 䈤᰾˖൘ӗ૱ᔰਁѻࡍˈᆏኡ䜭ޜਨԕ MON 87427 ⦹㊣㊭㋂Ѫ侢ᯉᡀ࠶ˈ࠶࡛ ሩ劬㊫઼㊫䘋㹼Ҷ㩕ޫᆖ䇴ԧˈᒦоᑨ㿴ሩ➗⦹㊣ᡆ৲➗⦹㊣㊭㋂ࡦᡀⲴ侢ᯉ லޫ㔃᷌䘋㹼∄䖳DŽ䙊䗷∄䖳࣐ᴹ䖜สഐ⦹㊣Ⲵ侢ᯉ઼࣐ᴹᑨ㿴⦹㊣Ⲵ侢ᯉ Ⲵᇎ傼ࣘ⢙൘⭏䮯઼ᆈ⍫⦷ᯩ䶒ᱟᆈ൘ᐞᔲˈӾ㘼⺞ᇊ䖜สഐ⦹㊣൘㩕ޫᆖᯩ 䶒ᱟоᑨ㿴⦹㊣ᆈ൘ᐞᔲDŽ⹄ウᣕ䈧㿱ᵜ⭣䈧䍴ᯉл䱴Ԧᢰᵟᣕ 17 ઼ ᢰᵟᣕ 18DŽ 劬㊫லޫᇎ傼 ⭘Ҿ⍻䈅Ⲵ劬㊫Ѫᯁ⛩৹ቮ几˄Ictalurus punctatus˅ˈᱟаӗҾे㖾⍢ Ⲵབྷර␑≤劬㊫ˈഐާᴹ伏ᵲᙗǃ⭏䮯ᘛǃ䘲ᓄᙗᕪㅹ⢩⛩㘼ᑨᑨ㻛⭘㩕ޫ⹄ ウ⍻䈅⭘劬㊫DŽ劬侢ᯉѝᡰਜ਼⦹㊣㊭㋂Ⲵ∄ֻ˄30%˅ᖃҾᡆ儈Ҿᐲ劬侢ᯉ ѝⲴ⦹㊣ਜ਼䟿DŽ࣐ᴹ䖜สഐ⦹㊣㊭㋂ǃሩ➗⦹㊣㊭㋂ᡆ৲➗⦹㊣㊭㋂Ⲵ劬侢ᯉˈ ަᙫփᡀ࠶оᐲᯁ⛩৹ቮ几劬Ⲵ侢ᯉᡀ࠶լDŽ侢ᯉ㓿䝽ࡦਾަ㋇㳻ⲭ䍘ਜ਼䟿 㓖Ѫ 32%ˈᒦਜ਼ᴹㅹ䟿≞DŽሩ劬侢ᯉ䘋㹼࠶᷀ˈᵚਁ⧠н਼㊫ර劬侢ᯉѻ䰤൘ᑨ 㿴㓴࠶ਜ਼䟿кᆈ൘ᱮ㪇ᐞᔲDŽ
⹄ウ⭘劬䍝㠚ᇶ㣿䟼ᐎྕຎཷ⎧┙Ⲵ Osage Catfisheries ⑄൪ѝ 2010 ᒤ 7 ᴸᆥ ॆⲴ䖳བྷаネ劬ˈഐަᮠ䟿䖳བྷˈнሩњփаа䘋㹼ᙗ࡛䢤ᇊDŽ䘀䘱ࡠ⍻䈅ᵪᶴ ਾ䇙劬㗔൘བྷර≤㇡ѝ䘲ᓄDŽ䘲ᓄᵏ䰤ˈᑨ㿴ல伏ᒦ䘋㹼≤䍘ⴁ⍻ˈ劬㗔ԕᐲ劬 侢ᯉѪ伏ˈல伏䟿㓖Ѫ⇿ཙ 3%փ䟽DŽ䘲ᓄйઘਾˈԫ࠶ࠪа㓴劬ˈޡ䇑 800 ᶑ 劬㻛䖜〫ࡠ⍻䈅⭘Ⲵ 20 њ≤㇡ѝ䘋㹼傟ॆDŽ䘲ᓄ傟ॆ䗷〻ᆼᡀਾˈњ≤㇡ѝⲴ 劬᤹➗փරབྷሿ࠶㓴DŽ⇿ 20 ᶑլփරⲴ劬˄Ѫ਼а㓴˅䘋㹼〠䟽ˈ❦ਾ᭮ എ≤㇡DŽ≤㇡ѻ䰤劬㗔њփⲴ䎧ᒣ൷փ䟽Ѫ 7.0-7.3 gDŽ20 њ⍻䈅⭘≤㇡㻛 䲿ᵪ࠶Ѫ 5 њ侢ல㓴ˈ⇿㓴 4 њ≤㇡ˈ⇿њ≤㇡ѝ 40 ᶑ劬DŽ ᡰᴹ劬侢ᯉࡦᡀਟл⊹Ⲵ仇㋂⣦ˈӾㅜ 0 ཙᔰˈ䘎㔝 8 ઘ⇿ཙੁ劬㗔ᣅல 䇱 40 min؍ᤱа㠤DŽᣅ᭮侢ᯉⲴ䗷〻؍ᤷᇊⲴ⍻䈅侢ᯉˈ⇿ཙⲴᣅலᰦ䰤ቭ䟿 䇱ᡰᴹⲴ劬䜭㜭൘ 40؍லᆼᡰᴹ≤㇡䟼Ⲵ劬ˈ⇿⅑侢ᯉᣅலⲴ䟿઼ᰦ䰤䰤䳄 min Ⲵᣅல䰤䳄䗮ࡠ価伏⣦ᘱDŽ൘⹄ウᔰࡽаཙǃ⹄ウㅜഋઘ઼ㅜޛઘᆼᡀ ਾ࠶࡛䘋㹼䇑ᮠ઼〠䟽DŽ⇿ᰕ䇠ᖅ劬Ⲵ侢ᯉ⎸㙇䟿ԕ৺ᇎ傼ࣘ⢙↫ӑᛵߥDŽ ሩ劬㊫Ⲵ⍻䈅㔃᷌䈧㿱ᵜ⭣䈧䍴ᯉл䱴Ԧᢰᵟᣕ 17DŽ⹄ウᮠᦞ㺘᰾ˈ 䈅㓴Ⲵᆈ⍫⦷Ѫ 100%ˈሩ➗㓴ѝᴹаֻ↫ӑˈᵚ㿲ሏࡠަԆ劬㗔Ⲵ↫ӑᡆᔲ⍻ ᑨ㹼ѪDŽᶕⓀҾ MON 87427 ⦹㊣㊭㋂Ⲵ劬侢ᯉо᧪ᴹᑨ㿴ሩ➗⦹㊣ᡆᐲ⦹㊣ Ⲵ劬侢ᯉ∄ˈሩᯁ⛩৹ቮ几劬Ⲵ侢ᯉ⎸㙇ǃփ䟽໎࣐ǃ侢ᯉ䖜ᦒ∄ǃᆈ⍫⦷৺ 㹼Ѫ㺘⧠⋑ᴹн㢟ᖡ૽DŽสҾ㿲ሏ㔃᷌ˈ䇔Ѫ MON 87427 ⦹㊣㊭㋂൘侢லᯁ⛩ ৹ቮ几劬ᰦˈоᑨ㿴⦹㊣㊭㋂൘㩕ޫᆖᯩ䶒ᇎ䍘ㅹ਼DŽ ㊫லޫᇎ傼 ⭘Ҿ⍻䈅Ⲵ㊫Ѫ୶⭘㚹呑૱˄Gallus domesticus˅ˈᱟа⭏䮯䙏ᓖ䖳 ᘛⲴᑨ㿱ᇦˈ䘲⭘Ҿ䘋㹼㩕ޫᆖ⹄ウDŽ⍻䈅ࣘ⢙൘᭦ࡠᰦ൷Ѫ 1 ᰕ喴㚹呑૱ Cobb × Cobb 500ˈ䳼䳴䲿ᵪDŽ䟷⭘䲿ᵪᆼޘ४㓴䇮䇑ሶ༴⨶࠶䝽㠣呑ㅬDŽሶ
XIV ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 MON 87427 ⦹㊣ǃᑨ㿴ሩ➗⦹㊣ԕ৺ 6 ৲➗⦹㊣૱Ⲵ㊭㋂࠶࡛࣐ᐕᡀ㊹ᵛˈ ԕ呑侢ᯉᴰ儈⦹㊣࣐ࡲ䟿ޕ侢ᯉѝ䘋㹼 42 ཙலޫDŽާփ࠶㓴ྲл㺘ᡰ⽪˖ ༴⨶1 ⦹㊣ḷ䇶 ⇿ᙗ࡛ ޜ呑ᮠⴞ ⇽呑ᮠⴞ ᙫ呑ᮠ/ ᙫ呑ᮠ/ ㅬᮠ /ㅬ2 /ㅬ2 ᙗ࡛ ༴⨶ 1 Pioneer 34P88 5 10 10 50 100 2 NK N72-G8 5 10 10 50 100 3 MON 87427 5 10 10 50 100 4 Middlekoop 4413 5 10 10 50 100 5 Garst 8450 5 10 10 50 100 6 Golden Harvest 5 10 10 50 100 H8920 7 DKC62-30 5 10 10 50 100 8 ᑨ㿴ሩ➗ 5 10 10 50 100
1 ⴤࡠᵜ⹄ウ⭏ᆈ䱦⇥㢲⇥ᆼᡀѻࡽ༴⨶ḷ䇶аⴤн⸕ᲃDŽ 2 ᇎ傼ᔰᰦ⇿ਚㅬᆀ൷ᴹ2ਚཊ։Ⲵ䳿呑ˈԕᕕ㺕ࡽ7ཙ⭡Ҿ↫ӑǃ侯↫઼〫ࠪⲴ䳿呑DŽ ྲIII. Cа㢲ᡰ䘠ˈԫօཊ։Ⲵ䳿呑൘ㅜ7ཙ〫ࠪDŽᖃ侢ᯉ䖜ॆ⦷઼փ䟽Ѫѫ㾱⹄ウᮠ DŽ൘㚹呑侢லᇎ傼ѝᑨᑨਁ⭏ഐ侕侯㘼↫઼〫ࠪ䳿呑⌅ڊᦞᰦˈ䘉ᱟ䈕⹄ウᇎ傼Ⲵḷ߶ ⲴᛵߥDŽ
䘎㔝லޫਾҾ 42 ᰕ喴〠䟽ˈ43-44 ᰕ喴ᰦ䟷⭘亸Ἶ㝡ս⌅༴↫ˈ䘋㹼࠶᷀DŽ ൘ᇎ傼ㅜ 0 ཙ˄᧕᭦ᖃཙ˅઼ 42 ཙ˄⭏ӗᙗ㜭䇴ԧ䱦⇥㔃ᶏ˅᤹ㅬѪ呑〠䟽DŽ 〠呑ㅬ᤹㓴࡛〠䟽ˈє㓴൘਼аཙ〠䟽DŽ䳿呑֙ᡤ㗵ḷˈነᇠ࣐ᐕѻࡽ・ণ䙀а 䟽DŽㅜ 21 ཙ઼ㅜ 42 ཙல伏ಘѝ࢙։Ⲵ侢ᯉ〠䟽ˈ呑ㅬѝⲴ侢ᯉᙫ䟿߿৫呑ㅬѝ Ⲵ侢ᯉˈ䇑㇇䟷伏䟿DŽ䇑㇇⭏ӗᙗ㜭ᮠᦞˈᙫ㔃ㅜ 42 ཙ⇿ਚ呑Ⲵᒣ൷໎䟽DŽᙫ 䟷伏䟿䲔ԕ䈕ㅬᆈ⍫呑Ⲵᙫ䟽䟿ˈᗇࠪㅜ 0-42 ཙⲴᒣ൷侢ᯉ:໎䟽∄DŽᙫ䟷伏䟿 䲔ԕᆈ⍫呑Ⲵ໎䟽࣐к↫ӑ⍫Ӿ䈕ㅬѝ〫ࠪⲴ呑໎䟽䇑㇇䈳ᮤ侢ᯉ:໎䟽∄ˈণ˖ ㅜ 0-42 ཙ䈳ᮤ侢ᯉ:໎䟽∄ = ㅜ 0-42 ཙ䟷伏䟿 ÷[(ㅜ 42 ཙㅬ䟽 – ㅜ 0 ཙㅬ䟽) +(ㅜ 0-42 ཙ↫ӑ/〫ࠪ呑Ⲵփ䟽 – ㅜ 0 ཙᒣ൷փ䟽)DŽ ሩ䇠ᖅ㔃᷌䘋㹼䲿ᵪᆼޘ४㓴䇮䇑ⲴҼഐ㍐ᯩᐞ࠶᷀ˈєњഐ㍐ᤷ侢ᯉ㊫ ර઼呑Ⲵᙗ࡛DŽާփ࠶᷀㔃᷌䈧㿱ᵜ⭣䈧䍴ᯉл䱴Ԧᢰᵟᣕ 18DŽ֯⭘ MON 87427ǃᑨ㿴ሩ➗ᡆ 6 њᑨ㿴৲➗⦹㊣ᵲӔ㊭㋂䝽ࡦⲴ侢ᯉ䘎㔝லޫ㚹呑 ⧠ཙˈަ䓛փᙗ㜭Ⲵ⍻ᇊ٬լˈণн਼ᶕⓀ⦹㊣侢ᯉሩ㚹呑Ⲵ䓛փᙗ㜭㺘 42 ⋑ᴹਁ⧠䶎亴ᵏⲴн㢟ᖡ૽DŽ༴↫ਾቨ䀓࠶᷀㔃᷌ҏ㺘᰾ˈԕн਼ᶕⓀ⦹㊣ᵲӔ ᒣᖃDŽ≥㊭㋂䝽ࡦⲴ侢ᯉ䘎㔝லޫ㚹呑 42 ཙˈަᇠᵰਾ䓛փ⍻ᇊ٬Ⲵᮠ٬ 㔬кˈ侢லਜ਼ MON 87427 侢ᯉ઼侢லਜ਼ᑨ㿴ሩ➗⦹㊣Ⲵ㚹呑䓛փᙗ㜭ǃነᇠ⦷ ᡆ㚹ᡀ࠶кнᆈ൘⭏⢙ޣᐞᔲDŽਜ਼ MON 87427 Ⲵ侢ᯉо࡙⭘ᑨ㿴ሩ➗ᡆ୶૱ DŽṩᦞ䘉Ӌ䍴ᯉޘڕ➗⦹㊣㊭㋂䝽ࡦⲴ侢ᯉ൘᭟ᤱ㚹呑ᘛ䙏⭏䮯㜭࣋ᯩ䶒аṧ৲ ਟԕᗇࠪ㔃䇪ᱟ MON 87427 оᑨ㿴⦹㊣൘㩕ޫкᱟ਼ⲴDŽ
XV ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߌъ䜘ޣҾMON 87427ᆹޘ䇴ԧ⭣䈧Ⲵഎ༽࠭ ˄2015ᒤ12ᴸ31ᰕ˅
XVI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ҿߌъ䜘ᢩ༽ؑ࠭Ⲵ䈤᰾ޣਨޜᆏኡ䜭
䰞仈˖ᙕᙗ∂ᙗ䈅傼ᵚ᤹➗ḷ߶lj㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼NJ˄ߌъ䜘 2031 ਧޜ -16-2013˅Ⲵ㾱≲ᆼᡀDŽ
䈤᰾˖㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣ MON 87427 ѝᕅޕҶⓀ㠚Ҿ൏༔ߌᵶ㧼 CP4 㧼 ṚⲴ cp4 epsps 㕆⸱ᒿࡇˈަ㺘䗮ӗ⢙ 5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦 ˄CP4 EPSPS˅ሩ㥹⭈㟖ⲴӢ઼ᙗ䶎ᑨվˈഐ↔㜭ཏ㙀ਇ㥹⭈㟖㊫䲔㥹ࡲDŽ ᆏኡ䜭ޜਨ൘ᔰਁ MON 87427 㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣Ⲵ䗷〻ѝˈ䘋㹼Ҷ བྷ䟿Ⲵᆹޘᙗ䇴ԧˈ䘉Ӌ䍴ᯉҾ 2012 ᒤ 7 ᴸ俆⅑ᨀӔ㔉ѝഭߌъ䜘ᇑṨDŽሩҾ ᵜ⅑᭦ࡠⲴޣҾᙕᙗ∂ᙗ䈅傼Ⲵ䰞仈ˈᡁޜਨ䲔ሩᴹᢰᵟ䍴ᯉ䘋а↕▴ཆˈ 䘈㺕ݵᨀӔҶᯠⲴ䈅傼ᮠᦞ˖࡙⭘བྷ㛐ᵶ㧼㌫㔏㺘䗮㓟ॆⲴ CP4 EPSPS 㳻ⲭሩ ሿ啐䘋㹼Ⲵᙕᙗ㓿ਓ∂ᙗ⹄ウDŽ䈕⹄ウᣕޘ᮷䈧㿱ᵜ⭣䈧Җлᢰᵟᣕ 15DŽ
ᴰᯠᆼᡀⲴCP4 EPSPSᙕᙗ∂ᙗ⹄ウ⭡SmedleyㅹӪҾ2016ᒤ2ᴸᆼᡀˈ䈅 ḷ߶ޣ傼䇮䇑ѫ㾱ᦞEPA OPPTS 870.1100 ˈҏ┑䏣ߌъ䜘亱ਁⲴ NY-2031-16-2013DŽ൘ᔪ・ҶἽ⢙ᶕⓀ㳻ⲭоབྷ㛐ᵶ㧼㺘䗮㌫㔏㓟ॆ㳻ⲭѻ䰤ᇎ 䍘ㅹ਼ᙗⲴสк˄ᢰᵟᣕ5˅DŽ⍻ᇊᗇࡠⲴབྷ㛐ᵶ㧼㺘䗮㓟ॆⲴCP4 EPSPS 㳻ⲭ㓟ᓖѪ92%ˈ㓿㓟ᓖṑ↓ਾⲴ♼㛳ⓦ⏢ѝCP4 EPSPS㳻ⲭⲴᇎ䱵⎃ᓖѪ83.9 mg/mLˈ䙊䗷㓿ਓᯩᔿঅᰕє⅑♼㛳䳼䳴10ਚCD-1ሿ啐ˈ⇿⅑♼㛳փ〟Ѫ33.3 mL/kgփ䟽˄䰤䳄བྷ㓖3-4ሿᰦ˅ˈԕ䗮ࡠ5000 mg/kgփ䟽Ⲵᙫࡲ䟿DŽ਼ᰦ䘋㹼䈅 傼Ⲵ䘈ᴹⓦࡲሩ➗㓴ԕ৺ԕ਼ㅹࡲ䟿ሿ⢋㹰㳻ⲭ˄BSA˅♼㛳Ⲵ㳻ⲭሩ➗㓴DŽ ᡰᴹࣘ⢙൘♼㛳ࡽ൷⾱伏༴⨶ˈ♼㛳ਾ⇿ཙє⅑㿲ሏሿ啐ᱟ↫ӑᡆᱟ㺘⧠ࠪѝ ∂⯷⣦ˈ㿲ሏᵏѪ14ཙDŽ䈖㓶ⲴѤᒺ㿲ሏᮠᦞवᤜ˖൘䲿ᵪ࠶㓴ѻᰕ˄ㅜ-2ཙ˅ 㿲ሏ1⅑ǃㅜ0ཙ♼㛳ࡽ㿲ሏ1⅑ǃㅜ0ཙ♼㛳ਾ㠣ቁ㿲ሏ2⅑˄ㅜа⅑♼㛳1ሿᰦ 1⅑ˈㅜҼ⅑♼㛳ਾ㓖2ሿᰦ1⅑˅ˈѻਾ˄ㅜ1~14ཙ˅⇿ཙ㠣ቁ㿲ሏ2⅑DŽ൘䲿ᵪ ࠶㓴ᖃཙ˄ㅜ-2ཙ˅ǃ⾱伏ࡽ˄ㅜ0ཙ˅ǃ♼㛳ࡽ˄ㅜ0ཙˈ⾱伏˅઼ㅜ7઼14ཙˈ ሩ⇿ਚሿ啐〠䟽DŽ൘ㅜ0~7ཙ઼ㅜ7~14ཙᇊ䟿⍻ᇊ⇿ਚሿ啐Ⲵਆ伏䟿DŽㅜ14ཙᰦ ሶᡰᴹࣘ⢙༴↫ˈ䘋㹼བྷփ䀓ࢆˈवᤜቨփ઼㚼㚹僘僬㌫㔏Ⲵ䇴ԧǃᡰᴹཆ㺘䶒 ઼ᆄਓǃ亵㞄઼བྷ㝁Ⲵཆ㺘䶒ˈԕ৺㜨㞄ǃ㞩㞄઼㞄оަޣⲴಘᇈ઼㓴㓷DŽ
⹄ウ㔃᷌㺘᰾ˈᇎ傼ᵏ䰤⋑ᴹਁ⭏↫ӑ⧠䊑ˈҏ⋑ᴹ㿲ሏࡠоਇ䈅⢙ޣ ⲴѤᒺ⯷⣦ˈᒦф൘փ䟽ǃփ䟽ਈॆǃᩴ伏䟿ᡆབྷփቨỰ㺘⧠ᯩ䶒⋑ᴹਁ⧠о CP4 EPSPSޣⲴᐞᔲDŽ⭡Ҿ൘ሿ啐ᆈ⍫⦷ǃѤᒺ㺘⧠ǃփ䟽ǃփ䟽໎࣐ǃ伏⢙ ⎸㙇ᡆབྷփ⯵⨶ᆖᯩ䶒൷ᵚਁ⧠о༴⨶ޣⲴн㢟৽ᓄˈഐ↔⺞ᇊCP4 EPSPS㳻 ⲭⲴᰐ᰾ᱮᦏᇣ⭘ࡲ䟿˄NOAEL˅㠣ቁѪ䈅傼ᴰ儈ࡲ䟿ণ5000 mg/kgփ䟽DŽ
ᓧ⣦ߥᶑḕᣕѻҼڕṩᦞ㘏ࠔ㤡઼ᶘᲃݹ˄2006˅൘ljѝഭት≁㩕ޫо ˄2002˅üü㟣伏о㩕ޫ㍐ᩴޕ⣦ߥNJࡇࠪⲴѝഭӪᒣ൷⎸䍩⦹㊣Ⲵ䈳ḕᛵߥˈ ѝഭӪ⇿ཙ⇿Ӫᒣ൷Ⲵ⦹㊣⎸䍩䟿Ѫ9.9 gˈṩᦞѝഭӪᒣ൷փ䟽㓖50 kgˈᣈ㇇ ᡀ⇿ཙ⇿ޜᯔփ䟽⎸䍩䟿ࡉѪ0.2 gDŽ㔃ਸMON 87427⦹㊣㊭㋂ѝCP4 EPSPS㳻ⲭ Ⲵᒣ൷㺘䗮≤ᒣ˄3.6 μg/g㓴㓷勌䟽˅ˈ⭡↔ՠ㇇ѝഭӪ㗔䙊䗷㟣伏ᩴޕ XVII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 MON 87427⦹㊣㊭㋂㘼ӗ⭏ⲴCP4 EPSPS㳻ⲭ᳤䵢MOE儈䗮6.9 × 106DŽഐ↔ˈᯠ 䘁ᆼᡀⲴሿ啐ᙕᙗ㓿ਓ♼㛳⹄ウ⅑䇱ᇎˈCP4 EPSPS㳻ⲭሩ䈅傼ሿ啐⋑ᴹн㢟 ᖡ૽ˈ䈕ᆹޘ♼㛳ࡲ䟿ᖃҾањᲞ䙊ᡀᒤӪ⇿ཙ伏⭘㓖69ⲴMON 87427⦹㊣ ㊭㋂㜭䗮ࡠⲴCP4 EPSPS㳻ⲭ᳤䵢≤ᒣDŽ
㠚Ӿ1996ᒤᣇ㥹⭈㟖བྷ䉶୶ъॆἽԕᶕˈਜ਼ᴹCP4 EPSPS㳻ⲭⲴߌӗ૱ 伏⭘DŽਜ਼ᴹCP4 EPSPSⲴᣇޘ伏⢙ᓄ䬮ѝᆈ൘ᒦ㻛Ӫ㊫઼ࣘ⢙ᆹ⨳ޘቡаⴤ൘ 㥹⭈㟖⢙⇿ᒤ䜭ᴹ30ཊњӗ૱൘13њഭᇦἽ䶒〟䎵䗷7400зޜ产˄Dill et al., 2008˅DŽ㠚1996ᒤᣇ㥹⭈㟖⢙୶ъॆἽԕᶕˈᐢᴹ䎵䗷1000ӯཤࣘ⢙㻛侢ல ⦷ᓧ઼⭏ӗڕ⢙ਜ਼ᴹ䖜สഐ⢙˄བྷ䜘࠶ᱟᣇ㥹⭈㟖⢙˅Ⲵ侢ᯉˈ⋑ᴹᖡ૽ࣘ ⲴᣕDŽаㇷ㓿䗷਼㹼䇴䇞ⲴޣҾ侢லਜ਼ᴹ䖜สഐ⢙˄वᤜᣇ㥹⭈㟖⢙˅侢 ᓧᖡ૽Ⲵ᮷⥞㔬䘠˄Eenennaam & Young, 2014˅ҏᤷࠪˈⴞڕᯉሩࣘ⢙⭏䮯઼ ᓧӗ⭏н࡙ᖡ૽DŽڕࡽ䘈⋑ᴹԫօֻᆀ㺘᰾ਜ਼ᴹ䖜สഐ⢙侢ᯉՊሩࣘ⢙⭏䮯઼ ൘䗷৫20ᒤˈޘ⨳ᐢ㓿ᴹ488亩⅑ޣҾCP4 EPSPS㳻ⲭᆹޘᙗⲴᇑḕᡆ䇴ᇑˈᗇ ᓧ⋑ᴹн㢟ᖡ૽DŽڕ⢙ࠪⲴ㔃䇪൷䇔ѪCP4 EPSPS㳻ⲭሩӪ㊫઼ࣘ
MON 87427⦹㊣ѝୟаᕅޕⲴᯠ㺘䗮ᱟCP4 EPSPS㳻ⲭˈоަԆᐢ㓿୶ъ ॆⲴ30ཊᣇ㥹⭈㟖⢙ѝ㺘䗮ⲴCP4 EPSPS㳻ⲭᆼޘ਼DŽᆏኡ䜭ޜਨ࡙⭘ሩ བྷ㛐ᵶ㧼㺘䗮㓟ॆⲴCP4 EPSPS㳻ⲭ䘋㹼Ҷཊ⅑ሿ啐ᙕᙗਓᴽ∂ᙗ⹄ウDŽ䘉Ӌ⹄ ウᒦ䶎ࠪҾሩCP4 EPSPS㳻ⲭᆹޘᙗᆈ⯁ˈ㘼ᱟࠪҾ䟽㿶ߌъ䜘уᇦ൘৽侸㿱 ѝᨀࠪⲴሩнㅖਸ䈅傼ḷ߶Ⲵᙕᙗ⹄ウ∂ᙗ⹄ウᯩ⌅Ⲵ⯁㲁DŽᡰᴹⲴᙕᙗਓᴽ∂ ᓧ⋑ᴹԫօн㢟ᖡ૽DŽڕᙗ⹄ウ㔃᷌৽༽䇱ᇎˈCP4 EPSPS㳻ⲭሩପңࣘ⢙Ⲵ
৲㘳᮷⥞˖ ᓧ⣦ߥᶑḕᣕѻҼ˄2002˅ˉ㟣伏о㩕ޫڕ㘏ࠔ㤡, ᶘᲃݹ. 2006. ѝഭት≁㩕ޫо .1 ㍐ᩴޕ⣦ߥ, pp. 21-33, Ӫ≁ছ⭏ࠪ⡸⽮. 2. lj䖜สഐἽ⢙ᆹޘ䇴ԧᤷইNJ˄ѝഭߌъ䜘ˈ2010˅ 3. Clive, J. 2014a. Global status of commercialized biotech/GM crops: 2014. ISAAA Brief No. 49. International Service for the Acquisition of Agri-Biotech Applications. Ithaca, NY. USA. 4. Codex Alimentarius (2009). Foods derived from modern biotechnology. Rome, Italy, Codex Alimentarius Commission, Joint FAO/WHO Food Standards Programme, Food and Agriculture Organization of the United Nations. Second Edition. 5. Eenennaam, A. L. v. and A. E. Young (2014). "Prevalence and impacts of genetically engineered feedstuffs on livestock populations." Journal of Animal Science 92(10): 4255-4278. 6. Kuiper, H. A., et al. (2013). "New EU legislation for risk assessment of GM food: no scientific justification for mandatory animal feeding trials." Plant Biotechnology Journal 11(7): 781-784.
XVIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߌъ䜘ޣҾMON 87427ᆹޘ䇴ԧ⭣䈧Ⲵഎ༽࠭ ˄2015ᒤ9ᴸ30ᰕ˅
XIX ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ҿߌъ䜘ᢩ༽ؑ࠭Ⲵ䈤᰾ޣਨޜᆏኡ䜭
䰞仈˖ᙕᙗ∂ᙗ䈅傼ᵚ᤹➗ḷ߶lj㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼NJ˄ߌъ䜘 2031 ਧޜ -16-2013˅Ⲵ㾱≲ᆼᡀDŽ
䈤᰾˖㠚 1996 ᒤㅜаֻ䖜สഐᣇ㥹⭈㟖བྷ䉶୶ъॆԕᶕˈCP4 EPSPS 㳻ⲭ䗴Ӻ Ⲵᯠ㺘䗮ᱟޕ⭘শਢDŽMON 87427 ⦹㊣ѝୟаᕅ֯ޘѪ→ᐢᴹ䘁 20 ᒤⲴᆹ CP4 EPSPS 㳻ⲭˈоަԆᐢ㓿୶ъॆⲴ 30 ཊᣇ㥹⭈㟖⢙ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭᆼޘ਼DŽ ڕ⢙㲭❦䘁 20 ᒤⲴ୶ъॆᓄ⭘䗷〻ѝᒦᵚਁ⧠ CP4 EPSPS 㳻ⲭሩӪ㊫઼ࣘ ᙗ䇴ԧ䍴ᯉˈ൘䇱ᇎབྷ㛐ᵶ㧼ޘ䶒Ⲵᆹޘᓧᴹԫօн࡙ᖡ૽ˈн䗷ˈѪҶᨀᴤ 㺘䗮㌫㔏ӗ⭏㳻ⲭо MON 87427 ⦹㊣ѝᯠ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭѻ䰤ާᴹㅹ਼ ᙗⲴสкˈᆏኡ䜭ޜਨ࡙⭘ሩབྷ㛐ᵶ㧼㺘䗮㓟ॆⲴ CP4 EPSPS 㳻ⲭ䘋㹼Ҷཊ ⅑ሿ啐ᙕᙗਓᴽ∂ᙗ⹄ウDŽ䘉Ӌ⹄ウᒦ䶎ࠪҾሩ CP4 EPSPS 㳻ⲭᆹޘᙗᆈ⯁ˈ ڕ৽ˈᡰᴹⲴᙕᙗਓᴽ∂ᙗ⹄ウ㔃᷌৽༽䇱ᇎˈCP4 EPSPS 㳻ⲭሩପңࣘ⢙Ⲵ ᓧ⋑ᴹԫօн㢟ᖡ૽DŽަѝˈᴰᯠᆼᡀⲴ⹄ウᦞ EPA OPPTS 870.1100 ઼ OECD Ự⍻ᤷই TG-425 䘋㹼DŽབྷ㛐ᵶ㧼㺘䗮㓟ॆⲴ CP4 EPSPS 㳻ⲭ䙊䗷㓿ਓᯩᔿԕ 2000 mg/kg փ䟽ࡲ䟿অ⅑♼㛳䳼䳴 10 ਚ CD-1 ሿ啐DŽ⭡Ҿ൘ሿ啐ᆈ⍫⦷ǃѤ ᒺ㺘⧠ǃփ䟽ǃփ䟽໎࣐ǃ伏⢙⎸㙇ᡆབྷփ⯵⨶ᆖᯩ䶒൷ᵚਁ⧠о༴⨶ޣⲴн 㢟৽ᓄˈഐ↔⺞ᇊ CP4 EPSPS 㳻ⲭⲴᰐ᰾ᱮᦏᇣ⭘ࡲ䟿˄NOAEL˅㠣ቁѪ䈅 傼ᴰ儈ࡲ䟿ণ 2000 mg/kg փ䟽DŽ 䪸ሩߌъ䜘Ҿ 2015 ᒤ 6 ᴸ઼ 9 ᴸє⅑ޣҾሿ啐ᙕᙗ∂ᙗ䈅傼ᮠᦞⲴ਼ṧ⯁ 䰞ˈᆏኡ䜭ޜਨ⅑䇔ⵏ⹄ウҶߌъ䜘 2031 ਧޜѝޣҾ㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅 傼Ⲵޣ㾱≲DŽᡁԜ⌘ࡠˈỰ⍻ḷ߶ѝ㿴ᇊҶᴰབྷ㙀ਇࡲ䟿⌅˄MTD˅઼ॺ ᮠ㠤↫ࡲ䟿⌅˄LD50˅DŽสҾ䇱ᦞᵳ䟽⌅ˈབྷ䟿Ⲵᆹޘᙗ䇴ԧ䍴ᯉԕ৺㠚 1996 ᒤԕᶕᣇ㥹⭈㟖⢙൘ޘц⭼㤳തⲴབྷ䶒〟Ἵ൷ਟ䇱ᇎ CP4 EPSPS 㳻ⲭⲴ 伏⭘ᆹޘᙗDŽഐ↔ˈLD50 ᯩ⌅ᒦн䘲⭘Ҿ䈨ྲ CP4 EPSPS 䘉ṧᰐᇣⲴ㳻ⲭDŽᆏ ኡ䜭ޜਨ৲➗Ҷഭ䱵к䙊㹼Ⲵ OECD Ự⍻ᤷই TG 425 ઼ EPA OPPTS 870.1100 ѝ᧕䘁Ҿᴰབྷ㙀ਇࡲ䟿⌅Ⲵ䲀ᇊࡲ䟿⌅˄Limit Dose˅ᶕ䘋㹼ᙕᙗ∂ᙗ䈅傼DŽ ؍OECD Ự⍻ᤷই TG 425 ᰾⺞ᤷࠪ˖ĀࠪҾሩࣘ⢙⾿࡙Ⲵ㘳㲁ˈ䲔䶎䈕䈅傼о ㌫ˈࡉн啃࣡֯⭘ GHS ⴞᖅ 5 㤳ത˄2000~5000ޣᣔӪ㊫ǃࣘ⢙৺⧟ຳᴹⴤ᧕ ਨ⋑ᴹ֯⭘ 2000 mg/kgޜmg/kg˅ᶕ䘋㹼ࣘ⢙䈅傼DŽā㘳㲁ࡠԕлһᇎˈᆏኡ䜭 ԕкⲴ♼㛳ࡲ䟿䘋㹼ሿ啐Ⲵᙕᙗ㓿ਓ∂ᙗ⹄ウ˖ ᓧᒦᰐডᇣDŽ㠚ڕ⢙ཊᒤⲴ୶ъॆᓄ⭘ᮠᦞ㺘᰾ CP4 EPSPS 㳻ⲭሩӪ઼ࣘ˅1 Ӿ 1996 ᒤᣇ㥹⭈㟖བྷ䉶୶ъॆἽԕᶕˈਜ਼ᴹ CP4 EPSPS 㳻ⲭⲴߌӗ૱ ˈ伏⭘DŽ൘䗷৫ 20 ᒤޘ伏⢙ᓄ䬮ѝᆈ൘ᒦ㻛Ӫ㊫઼ࣘ⢙ᆹ⨳ޘቡаⴤ൘ ޘ⨳ᐢ㓿ᴹ 488 亩⅑ޣҾ CP4 EPSPS 㳻ⲭᆹޘᙗⲴᇑḕᡆ䇴ᇑˈᗇࠪⲴ㔃 ᓧ⋑ᴹн㢟ᖡ૽DŽṩᦞഭ䱵ߌъڕ⢙䇪൷䇔Ѫ CP4 EPSPS 㳻ⲭሩӪ㊫઼ࣘ ⭏⢙ᢰᵟ㓴㓷˄ISAAA˅ᴰᯠⲴᙫ㔃ᣕˈࡠ 2014 ᒤˈঅ㓟ᣇ䲔㥹ࡲ ᙗ⣦Ⲵ䖜สഐ⢙Ἵ䶒〟儈䗮 1.03 ӯޜ产ˈਖᴹ 0.51 ӯޜ产ᣇ㲛ᣇ䲔㥹
XX ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ࡲ༽ਸᙗ⣦⢙ˈޡ䇑ঐޘ⨳䖜สഐ⢙Ἵᙫ䶒〟Ⲵ㓖 85%ˈᒦᴹ䎵䗷 1000 ӯཤࣘ⢙㻛侢லਜ਼ᴹ䖜สഐ⢙˄བྷ䜘࠶ᱟᣇ㥹⭈㟖⢙˅Ⲵ侢ᯉDŽ 2˅䲿ᵜ⭣䈧ҖᨀӔⲴབྷ䟿ᆹޘᙗ䇴ԧᮠᦞҏ䏣ԕ䇱ᇎ CP4 EPSPS 㳻ⲭⲴᆹޘ ᓧᴹн࡙ڕᙗ˖CP4 EPSPS оᐢ⸕Ⲵ∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃ䗷ᡆሩ ᖡ૽Ⲵ⭏⢙⍫ᙗ㳻ⲭѻ䰤൷нާᴹ㔃ᶴլᙗ˗CP4 EPSPS оӪ㊫઼ࣘ⢙ ᰕᑨ伏⭘Ⲵᒦᐢ䇱᰾ᱟᆹޘⲴἽ⢙㳻ⲭ˄ྲἽ⢙Ⓚ EPSPS 㳻ⲭ˅ᴹᖸ儈 ਼Ⓚᙗˈ㘼䘉Ӌ㳻ⲭ൷ާᴹ䮯ᵏᆹޘ伏⭘শਢ˗CP4 EPSPS 㳻ⲭሩ✝нっ ᇊˈ䶎ᑨᇩ᱃൘⦹㊣✩ࡦ䗷〻ѝཡ⍫ˈᒦ൘⎸ॆ⏢ѝՊ㻛䗵䙏⎸ॆᦹˈн ާᴹ㌫㔏ᙗ᳤䵢䰞仈DŽ 3˅ṩᦞѝഭӪᒣ൷⎸䍩⦹㊣Ⲵ䈳ḕᛵߥ˄↓᮷ㅜ 95 亥㺘 21˅ˈԕѝഭӪᒣ൷ փ䟽㓖 50 kg 䇑ˈ⇿ཙ⇿ޜᯔփ䟽Ⲵ⦹㊣⎸䍩䟿ࡉѪ 0.2 gDŽ㔃ਸ MON 87427 ⦹㊣㊭㋂ѝ CP4 EPSPS 㳻ⲭⲴᒣ൷㺘䗮≤ᒣ˄3.6 μg/g 㓴㓷勌䟽˅ˈ⭡↔ՠ ㇇ѝഭӪ㗔䙊䗷㟣伏ᩴޕ MON 87427 ⦹㊣㊭㋂㘼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ нਟ㜭䗮ࡠޕ᳤䵢 MOE 儈䗮 2.76 × 106DŽ䘉аᮠ٬ᖃབྷˈ䙊ᑨⲴ㟣伏ᩴ 䈕ᮠ䟿㓗Ⲵ᳤䵢䟿DŽ ഐ↔ˈਜ਼ᴹ CP4 EPSPS 㳻ⲭⲴߌӗ૱ᐢ㓿൘ޘ⨳㤳ത㻛ᆹޘᓄ⭘Ҷ䘁 20 ᙗ䇱ޘҾ CP4 EPSPS Ⲵᆹޣᒤˈᴤ儈♼㛳ࡲ䟿Ⲵᙕᙗ∂ᙗ⹄ウᒦн㜭ᨀᴤཊ ᦞDŽ↔ཆˈ儈ࡲ䟿♼㛳ᖰᖰ൘ᢰᵟк䳮ԕᇎ⧠˖ഐ㳻ⲭ䍘ⓦ䀓ᓖᴹ䲀ˈ㾱䗮ࡠ儈 ࡲ䟿♼㛳ᗵ享ᨀ儈♼㛳փ〟ᡆᱟ໎࣐♼㛳⅑ᮠˈ㘼䘉єᯩᔿਟ㜭䜭Պᕅ䎧ᇎ傼 ࣘ⢙нᗵ㾱Ⲵᓄ◰࣋ˈᖸᴹਟ㜭䇙ᇎ傼㔃᷌⏶нDŽ㔬кᡰ䘠ˈޘ䶒Ⲵ伏⭘ ⭘শਢ൷ᐢ䇱ᇎҶ CP4 EPSPS 㳻ⲭⲴ伏⭘઼侢⭘ᆹ֯ޘᙗ䇴ԧ઼䮯ᵏⲴᆹޘᆹ ޘᙗˈᯠ䘁ᆼᡀⲴሿ啐ᙕᙗ㓿ਓ∂ᙗ⹄ウ˄ᢰᵟᣕ 14˅ҏа⅑䇱ᇎҶ䖜ส ᓧᒦ⋑ᴹн㢟ᖡ૽DŽڕ⢙ഐ⦹㊣ MON 87427 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭሩӪ㊫઼ࣘ
৲㘳᮷⥞˖ ᓧ⣦ߥᶑḕᣕѻҼ˄2002˅ˉ㟣伏о㩕ޫڕ㘏ࠔ㤡, ᶘᲃݹ. 2006. ѝഭት≁㩕ޫо .1 ㍐ᩴޕ⣦ߥ, pp. 21-33, Ӫ≁ছ⭏ࠪ⡸⽮. 2. lj䖜สഐἽ⢙ᆹޘ䇴ԧᤷইNJ˄ѝഭߌъ䜘ˈ2010˅ 3. Clive, J. 2014a. Global status of commercialized biotech/GM crops: 2014. ISAAA Brief No. 49. International Service for the Acquisition of Agri-Biotech Applications. Ithaca, NY. USA. 4. Codex Alimentarius (2009). Foods derived from modern biotechnology. Rome, Italy, Codex Alimentarius Commission, Joint FAO/WHO Food Standards Programme, Food and Agriculture Organization of the United Nations. Second Edition. 5. Kuiper, H. A., et al. (2013). "New EU legislation for risk assessment of GM food: no scientific justification for mandatory animal feeding trials." Plant Biotechnology Journal 11(7): 781-784.
XXI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߌъ䜘ޣҾMON 87427ᆹޘ䇴ԧ⭣䈧Ⲵഎ༽࠭ ˄2015ᒤ6ᴸ24ᰕ˅
XXII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ҿߌъ䜘ᢩ༽ؑ࠭Ⲵ䈤᰾ޣਨޜᆏኡ䜭
䰞仈˖ᙕᙗ∂ᙗ䈅傼ᵚ᤹➗ḷ߶lj㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼NJ˄ߌъ䜘 2031 ਧޜ -16-2013˅Ⲵ㾱≲ᆼᡀDŽ
䈤᰾˖㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣ MON 87427 ѝᕅޕҶⓀ㠚Ҿ൏༔ߌᵶ㧼 CP4 㧼 ṚⲴ cp4 epsps 㕆⸱ᒿࡇˈަ㺘䗮ӗ⢙ 5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦 ˄CP4 EPSPS˅ሩ㥹⭈㟖ⲴӢ઼ᙗ䶎ᑨվˈഐ↔㜭ཏ㙀ਇ㥹⭈㟖㊫䲔㥹ࡲDŽᆏኡ 䜭ޜਨ൘ᔰਁ MON 87427 㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣Ⲵ䗷〻ѝˈ䘋㹼Ҷབྷ䟿Ⲵᆹ ޘᙗ䇴ԧˈ䘉Ӌ䍴ᯉҾ 2012 ᒤ 7 ᴸ俆⅑ᨀӔ㔉ѝഭߌъ䜘ᇑṨDŽሩҾᵜ⅑᭦ࡠ ਨ䲔ሩᴹᢰᵟ䍴ᯉ䘋а↕▴ཆˈ䘈㺕ݵᨀޜҾᙕᙗ∂ᙗ䈅傼Ⲵ䰞仈ˈᡁޣⲴ ӔҶᯠⲴ䈅傼ᮠᦞ˖࡙⭘བྷ㛐ᵶ㧼㌫㔏㺘䗮㓟ॆⲴ CP4 EPSPS 㳻ⲭሩሿ啐䘋㹼 Ⲵᙕᙗ㓿ਓ∂ᙗ⹄ウDŽ䈕⹄ウᣕޘ᮷䈧㿱ᵜ⭣䈧Җлᢰᵟᣕ 14DŽ 䈕亩ᯠⲴ CP4 EPSPS ᙕᙗ∂ᙗ⹄ウ⭡ Smedley ㅹӪҾ 2015 ᒤ 6 ᴸᆼᡀˈ䈅 ┑㾱≲а㠤ˈҏޣ傼䇮䇑ѫ㾱ᦞ EPA OPPTS 870.1100ˈо OECD TG 425 ѝ 䏣ߌъ䜘亱ਁⲴޣḷ߶ NY-2031-16-2013DŽߌъ䜘ޣҾ㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼 Ⲵḷ߶ѝ᧿䘠Ҷєᯩ⌅˖LD50 ઼ MTDDŽަѝˈLD50 ⌅ᒦн䘲⭘Ҿ䖜สഐ⢙ ѝᯠ㺘䗮㳻ⲭⲴᆹޘᙗ䇴ԧDŽѪޣ䭞Ⲵ㩕ޫ⢙䍘ˈ㔍བྷ䜘࠶㳻ⲭ䍘䜭ᱟᰐ∂ⲴDŽ བྷ䜘࠶ᩴޕⲴ㳻ⲭ䍘䜭Պ㓿䗷≤䀓઼㳻ⲭ䞦Ⲵ䱽䀓䗷〻ˈਈᡀঅњ≘ส䞨ᡆ⸝䬮 㛭⇥ˈᴰ㓸൘փ⭘Ҿ㳻ⲭ䍘ⲴਸᡀᡆަԆԓ䉒䙄ᖴ˄Delaney, Astwood et al. 2008˅DŽ伏⢙ѝⲴ㔍བྷཊᮠ㳻ⲭ䍘䜭䘋㹼ҶᆹޘᙗỰ⍻ˈ൷ᵚਁ⧠ާᴹ∂ᙗ ˄Hammond and Cockburn 2008˅DŽഐ↔ˈӪ㊫㟣伏ѝࣘ⢙઼Ἵ⢙ᶕⓀⲴ㳻ⲭ䍘 ൷ާᴹ䮯ᵏᆹޘ伏⭘শਢDŽᒦфˈ㠚 1996 ᒤ䎧ᐢሩ䎵䗷 25 н਼Ⲵ䖜สഐ㳻ⲭ 䘋㹼Ҷᙕᙗ∂ᙗ⹄ウˈᵚਁ⧠о⍻䈅⢙ޣⲴሿ啐䶎↓ᑨ↫ӑ˄CERA, 2015˅ ٬DŽഐ↔ˈMTD ᯩ⌅ᱟ⭘Ҿ䇴ՠ䖜 㘼ᵚ㿲ሏࡠࣘ⢙↫ӑণણ⵰ᰐ⌅⍻ᇊ LD50 สഐ⢙ѝ㳻ⲭ䍘ԫօ▌൘ডᇣⲴᴤѪ、ᆖⲴᯩ⌅DŽ NY-2031-16-2013 ѝޣҾ MTD Ⲵᯩ⌅ᒦ⋑ᴹ㿴ᇊᴰվਟ᧕ਇࡲ䟿≤ᒣˈ䘉 оഭཆⲴ䈅傼ḷ߶઼ᤷইᱟа㠤ⲴDŽ൘ᯠⲴᙕᙗ∂ᙗ⹄ウⲴࡲ䟿䇮ᇊᰦˈᡁԜ ৲㘳Ҷ EPA OPPTS 870.1100 ઼ OECD TG 425 ѝⲴ䲀ᇊࡲ䟿⌅˄limit dose˅DŽ EPA OPPTS 870.1100 ઼ OECD TG 425 ൷Ѫ䙊⭘Ⲵᙕᙗ∂ᙗ䈅傼ᤷইˈ㘼нᱟ⢩ ࡛䪸ሩ㳻ⲭᙕᙗ∂ᙗ⹄ウࡦᇊⲴDŽOECD TG 425 ѝ⢩࡛ᤷࠪˈࠪҾࣘ⢙⾿࡙Ⲵ ᓧᴹⴤ᧕ᖡ૽ˈнڕ⢙㘳㲁ˈྲ᷌ᴹ䍴ᯉ㜭ཏ㺘᰾⍻䈅⢙䍘нཚਟ㜭ሩӪ㊫ᡆࣘ 啃࣡䘋㹼䎵䗷 2000 mg/kg փ䟽Ⲵ䲀ᇊࡲ䟿⌅⹄ウDŽ䲔Ҷࣘ⢙⾿࡙ഐ㍐ཆˈаӋ о㳻ⲭⓦ䀓ᓖ઼♼㛳ࡦࡲࡦ༷ޣⲴᢰᵟᙗഠ䳮ҏਟ㜭䲀ࡦᙕᙗ∂ᙗ䈅傼ѝ㳻 ⲭⲴᴰ儈♼㛳ࡲ䟿˄ণׯᱟ䗮ࡠ 2000 mg/kg bw ҏᖸഠ䳮˅DŽ儈ࡲ䟿♼㛳䴰㾱ࡦ ࡠDŽ䘉ᛵߥлᓄᖃቭᴰབྷਟ㜭ڊ儈ᓖ⎃㕙ⲴࡦࡲˈሩᴹӋ㳻ⲭ㘼䀰ਟ㜭ᖸ䳮༷ MOE˄Bushey, Bannon et al. 2014˅DŽ↔ཆˈ䘈ᴹަԆаӋо֯ 䲀٬ޘൠᨀ儈ᆹ ⭘儈ᓖ⎃㕙㳻ⲭޣⲴᢰᵟ䳮仈DŽ∄ྲḀӋ㳻ⲭ൘♼㛳ⓦࡲѝⲴⓦ䀓ᓖᴹ䲀ˈᰐ ⌅ᙫᱟ䗮ࡠ g/kg ᮠ䟿㓗Ⲵࡲ䟿≤ᒣㅹㅹDŽ⭡Ҿབྷ䟿Ⲵᆹޘᙗ䇴ԧ䍴ᯉ൷㺘᰾ˈ ᓧ⋑ᴹн㢟ᖡ૽ˈഐ↔ˈᡁԜ䘹ᤙҶ 2000 mg/kg փڕ⢙CP4 EPSPS ሩӪ㊫઼ࣘ 䟽Ⲵ♼㛳ࡲ䟿⅑ሩ CP4 EPSPS 䘋㹼ᙕᙗ∂ᙗ⹄ウDŽᒦфˈ䈕ࡲ䟿ᖃҾᡀᒤ
XXIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ӫ⇿ᰕ伏⭘㓖 27.6 Ⲵ MON 87427 ⦹㊣㊭㋂ˈ䘉аࡲ䟿ԓ㺘Ҷ㟣伏ᩴޕнਟ㜭 䗮ࡠⲴ≤ᒣDŽ Smedley˄2015˅⹄ウᱟ൘ᔪ・Ҷབྷ㛐ᵶ㧼㺘䗮㌫㔏ӗ⭏㳻ⲭо MON 87427 ⦹㊣ѝᯠ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭѻ䰤Ⲵㅹ਼ᙗสк˄ᢰᵟᣕ 5˅ˈ࡙⭘བྷ㛐 ᵶ㧼㺘䗮㓟ॆⲴ CP4 EPSPS 㳻ⲭԕ㓿ਓᯩᔿԕ 2000 mg/kg փ䟽ࡲ䟿অ⅑♼㛳䳼 䳴 10 ਚ CD-1 ሿ啐DŽ਼ᰦ䘋㹼䈅傼Ⲵ䘈ᴹⓦࡲሩ➗㓴ԕ৺ԕ਼ㅹࡲ䟿ሿ⢋㹰 㳻ⲭ˄BSA˅♼㛳Ⲵ㳻ⲭሩ➗㓴DŽᡰᴹࣘ⢙൘♼㛳ࡽ൷⾱伏༴⨶ˈ♼㛳ਾ⇿ཙ є⅑㿲ሏሿ啐ᱟ↫ӑᡆᱟ㺘⧠ࠪѝ∂⯷⣦ˈ㿲ሏᵏѪ 14 ཙDŽㅜ 14 ཙᰦሶᡰᴹ ࣘ⢙༴↫ˈ䘋㹼བྷփ䀓ࢆDŽ⹄ウ㔃᷌㺘᰾ˈᰐ䇪Ӿሿ啐ᆈ⍫⦷ǃѤᒺ㺘⧠ǃփ䟽ǃ փ䟽໎࣐ǃ伏⢙⎸㙇ᡆབྷփ⯵⨶ᆖᯩ䶒ⴻˈ൷ᵚਁ⧠о༴⨶ޣⲴн㢟৽ᓄDŽഐ ↔ˈ䙊䗷䈕ሿ啐ᙕᙗ∂ᙗ⹄ウᗇࡠⲴ CP4 EPSPS 㳻ⲭⲴᰐ᰾ᱮᦏᇣ⭘ࡲ䟿 ˄NOAEL˅㠣ቁѪ⹄ウᴰ儈ࡲ䟿 2000 mg/kg փ䟽ˈণ CP4 EPSPS 㳻ⲭⲴ㓿ਓᙕ ᙗ∂ᙗᴰབྷ㙀ਇࡲ䟿Ѫ 2000 mg/kg փ䟽DŽṩᦞ㘏ࠔ㤡઼ᶘᲃݹ˄2006˅൘ljѝ ߥNJࡇࠪ⣦ޕᓧ⣦ߥᶑḕᣕѻҼ˄2002˅üü㟣伏о㩕ޫ㍐ᩴڕഭት≁㩕ޫо ⲴѝഭӪᒣ൷⎸䍩⦹㊣Ⲵ䈳ḕᛵߥˈѝഭӪ⇿ཙ⇿Ӫᒣ൷Ⲵ⦹㊣⎸䍩䟿Ѫ 9.9 gˈ ṩᦞѝഭӪᒣ൷փ䟽㓖 50 kgˈᣈ㇇ᡀ⇿ཙ⇿ޜᯔփ䟽⎸䍩䟿ࡉѪ 0.2 gDŽ㔃ਸ MON 87427 ⦹㊣㊭㋂ѝ CP4 EPSPS 㳻ⲭⲴᒣ൷㺘䗮≤ᒣ˄3.6 μg/g 㓴㓷勌䟽˅ˈ ⭡↔ՠ㇇ѝഭӪ㗔䙊䗷㟣伏ᩴޕ MON 87427 ⦹㊣㊭㋂㘼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ ᳤䵢 MOE 儈䗮 2.76 × 106DŽഐ↔ˈᯠ䘁ᆼᡀⲴሿ啐ᙕᙗ㓿ਓ♼㛳⹄ウ⅑䇱ᇎˈ CP4 EPSPS 㳻ⲭሩ䈅傼ሿ啐⋑ᴹн㢟ᖡ૽ˈ䈕ᆹޘ♼㛳ࡲ䟿ᖃҾањᲞ䙊ᡀᒤ Ӫ⇿ཙ伏⭘㓖 27.6 Ⲵ MON 87427 ⦹㊣㊭㋂㜭䗮ࡠⲴ CP4 EPSPS 㳻ⲭ᳤䵢 ≤ᒣDŽ ሩҾ䖜สഐ⢙Ⲵᆹޘᙗ䇴ԧˈवᤜѝഭ൘Ⲵޘ⨳ⴁ㇑ᵪᶴⲴ⌅㿴ᤷই ѝ൷ᤷࠪˈᦞњṸ࠶᷀ࡉᨀᙕᙗ∂⨶ᆖ䍴ᯉˈᖃ䇱ᦞᵳ䟽࠶᷀ᯩ⌅㜭ཏ㺘 ⭘শਢᰦˈᙕᙗ∂⨶ᆖ䍴ᯉᒦ䶎ᗵ䴰DŽྲ֯ޘ᰾䖜สഐ⭏⢙ѝᯠ㺘䗮㳻ⲭާᴹᆹ ߌъ䜘亱ᐳⲴlj䖜สഐἽ⢙ᆹޘ䇴ԧᤷইNJ˄2010˅ѝ㿴ᇊĀᖃᯠ㺘䗮㳻ⲭ䍘ᰐ ᙗ䍴ᯉн䏣ᰦˈᗵ享ᨀᙕᙗ㓿ਓ∂ᙗ䍴ᯉāDŽ਼ṧˈ䍏䍓ޘ伏⭘শਢˈᆹޘᆹ ᢗ㹼㋞ߌ㓴㓷/ц⭼ছ⭏㓴㓷㚄ਸ伏૱ḷ߶䇑ࡂⲴ伏૱⌅ިငઈՊ Codex ҏਁᐳ Ҷഭ䱵Პ䙽䟷⭘Ⲵᤷই˄Codex Alimentarius 2009˅ˈ᧘㦀֯⭘䇱ᦞᵳ䟽⌅ሩ䖜 สഐἽ⢙ᶕⓀ伏૱䘋㹼ᆹޘᙗ䇴ՠDŽ2009 ᒤ⡸Ⲵ Codex ޡ䇶᮷Ԧѝᤷࠪ˖Āྲ ᷌伏⢙ѝᆈ൘Ⲵ㳻ⲭоᐢ⸕ਟᆹޘ伏⭘㳻ⲭѻ䰤ᒦᰐլᙗˈਟ㜭䴰㾱䘋㹼䘲ᖃ Ⲵ㓿ਓ∂⨶ᆖ⹄ウāDŽѪޣ䭞Ⲵ㩕ޫ⢙䍘ˈ㔍བྷ䜘࠶㳻ⲭ䍘䜭ᱟᰐ∂ⲴDŽਚᴹ ḀӋ⭏⢙փӗ⭏Ⲵᶱቁᮠ㳻ⲭ䍘ᱟᐢ⸕ᴹ∂ⲴDŽབྷ䜘࠶ᴹ∂㳻ⲭ䜭ᱟ䙊䗷ᙕᙗ ⭘ᵪࡦӗ⭏∂ᙗ˄Sjoblad, McClintock et al. 1992, Hammond and Fuchs 1998, Pariza and Johnson 2001˅DŽҏᴹᶱቁᮠ㳻ⲭֻཆˈѫ㾱ᱟḀӋᣇ㩕ޫ㳻ⲭˈྲἽ⢙ࠍ ↺⢩䳶㍐DŽн䗷ˈ䙊䗷⭏⢙ؑᆖᯩ⌅ਟԕ⺞䇔䖜สഐ⢙ѝᯠ㺘䗮㳻ⲭо䘉Ӌ 㳻ⲭᱟާᴹ㔃ᶴլᙗ˄Hammond, et al. 2013˅DŽ Smedley˄2015˅⹄ウᒦ䶎⭡Ҿሩ CP4 EPSPS 㳻ⲭⲴᆹޘᙗᆈ⯁ˈ㘼ᱟࠪҾ 䟽㿶ߌъ䜘уᇦ൘৽侸㿱ѝᨀࠪⲴሩнㅖਸ䈅傼ḷ߶Ⲵᙕᙗ⹄ウ∂ᙗ⹄ウᯩ ⌅Ⲵ⯁㲁DŽо亴ᵏ㔃᷌а㠤ˈSmedley˄2015˅Ⲵ⹄ウ㔃᷌䘋а↕䇱ᇎҶѻࡽᐢ ᙗ㔃䇪DŽޘҾ CP4 EPSPS 㳻ⲭⲴᆹޣ㓿ᨀ㔉ߌъ䜘Ⲵєԭᙕᙗ∂ᙗ⹄ウᣕѝ XXIV ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ѻࡽሩ CP4 EPSPS 㳻ⲭ䘋㹼Ⲵᙕᙗ∂ᙗ⹄ウ˄࠶࡛Ѫ 1993 ᒤ Hammond Ⲵ ⹄ウ઼ 2002 ᒤ Bonnette Ⲵ⹄ウ˅൷ᦞ EPA OPPTS 870.1100 ᤷই઼ഭ䱵䙊⭘ Ⲵ OECD Test Guideline 425 ᤷই䘋㹼DŽHammond Ҿ 1993 ᒤᆼᡀⲴ⹄ウѝˈCP4 EPSPS 㳻ⲭԕঅаࡲ䟿ਓᴽ♼㛳а㓴䳼䳴 10 ਚ CD-1 ሿ啐ˈ਼ᰦ䇮а㓴լ 㳻ⲭࡲ䟿≤ᒣⲴሩ➗ሿ㓴ˈԕሿ⢋㹰㳻ⲭ˄BSA˅ਓᴽ♼㛳ሿ啐˗♼㛳ࡽ䇙ሿ 啐⾱伏オ㞩ˈ⇿ཙє⅑㿲ሏᱟᴹሿ啐↫ӑᡆᱟѝ∂⯷⣦ˈ㿲ሏᵏ࠶࡛Ѫ㔉㦟ਾ 7 ཙ˄Hammond, 1993˅઼ 14 ཙ˄Bonnette, 2002˅˗⍫փ㿲ሏ㔃ᶏਾˈሶሿ啐༴ ↫ᒦ䘋㹼བྷփ䀓ࢆDŽHammond˄1993˅ᙕᙗ∂ᙗ⹄ウѝ䘹ᤙ 572 mg/kg bw Ⲵࡲ ᆸՠ㇇Ⲵ⇿ᰕ᳤䵢䟿ᶑԦлӽ❦ާᴹ䏣ཏབྷⲴ᳤䵢؍൘儈ᓖ؍⺞䟿≤ᒣᱟѪҶ ᆸՠ㇇ѝᒦ⋑ᴹवᤜ伏⢙൘࣐ᐕǃ✩ࡦԕ৺⎸ॆ䗷〻؍㘼൘⇿ᰕ᳤䵢䟿Ⲵˈ䲀٬ ѝਁ⭏䱽䀓ሬ㠤ᴰਾӪփᖸ䳮ሩᆼᮤ㳻ⲭާᴹᴹѹⲴ᳤䵢DŽਖа亩ޣҾ CP4 EPSPS 㳻ⲭⲴᙕᙗ⹄ウᱟ NK063 ѝ㺘䗮Ⲵ CP4 EPSPS L214P 㳻ⲭˈަ⹄ウᣕ ᙗᨀᴤཊޘҏҾ 2015 ᒤ 3 ᴸᨀӔ㔉ѝഭߌъ䜘ˈ⭘ҾѪ CP4 EPSPS 㳻ⲭⲴᆹ Ⲵ䇱ᦞ˄Bonnette, 2002˅DŽ䘉а⹄ウਟⴤ᧕⭘Ҿր䇱 MON 87427 ⦹㊣ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭˈഐѪє㘵ӵᴹањ≘ส䞨Ⲵᐞᔲˈ㕆⸱ᒿࡇլᙗ DŽ ൘䖳ᯠⲴ⹄ウ˄Bonnette, 2002˅ѝˈ㿲ሏᵏѪ 14 ཙˈ♼㛳ࡲ䟿ҏᴤ儈˄817 mg/kg bw˅DŽ䘉Ӌ⹄ウᒦн䘍㛼ѝഭޣᤷই NY-2031-16-2013 ѝሩ䖜สഐ⭏⢙ᯠ㺘 䗮㳻ⲭᙕᙗ∂ᙗ࠶᷀Ⲵ䈅傼䇮䇑㾱≲˖䘉Ӌ⹄ウ⺞ᇊᴰ儈♼㛳ࡲ䟿Ѫᴰབྷ㙀ਇࡲ 䟿˄MTD˅ˈᒦሩѻࡽᐢ⭡䇱ᦞᵳ䟽࠶᷀⌅⺞ᇊⲴ MON 87427 ѝ㺘䗮 CP4 EPSPS ᙗᨀҶ䘋а↕䇱ᦞDŽޘ㳻ⲭⲴᆹ བྷ䟿Ⲵᆹޘᙗ䇴ԧ䍴ᯉԕ৺㠚 1996 ᒤԕᶕᣇ㥹⭈㟖⢙൘ޘц⭼㤳തⲴ བྷ䶒〟Ἵ൷ਟ䇱ᇎ CP4 EPSPS 㳻ⲭⲴ伏⭘ᆹޘᙗDŽᵜ⭣䈧ҖѝสҾ䇱ᦞᵳ䟽 ⌅ሩ MON 87427 ⦹㊣䘋㹼Ⲵ伏⭘ᆹޘᙗ䇴ԧ䍴ᯉवᤜ˖ ⭏⢙ؑᆖ࠶᷀㺘᰾ˈCP4 EPSPS оᐢ⸕∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃ䗷˅1 ˗˅ᓧᴹн࡙ᖡ૽Ⲵ⭏⍫⍫ᙗ㳻ⲭѻ䰤нާᴹ㔃ᶴլᙗ˄ᢰᵟᣕ 6ڕᡆሩ 2˅CP4 EPSPS оӪ㊫઼ࣘ⢙ᰕᑨ伏⭘Ⲵᒦᐢ䇱᰾ᱟᆹޘⲴἽ⢙㳻ⲭ˄ྲἽ ⢙Ⓚ EPSPS 㳻ⲭ˅ᴹᖸ儈਼Ⓚᙗˈ㘼䘉Ӌ㳻ⲭ൷ާᴹ䮯ᵏᆹޘ伏⭘শਢ˗ 3˅փཆ⎸ॆ䈅傼㺘᰾ˈCP4 EPSPS 㳻ⲭ൘⁑ᤏ㛳⏢˄SGF˅ѝՊ㻛䗵䙏⎸ ॆᦹˈнާᴹ㌫㔏ᙗ᳤䵢䰞仈˄ᢰᵟᣕ 8˅DŽ 4˅CP4 EPSPS 㳻ⲭሩ✝нっᇊˈ㘼ᰕᑨ㟣伏ѝ⦹㊣䙊ᑨ䴰㾱✩ࡦˈ߿ቁҶ ާᴹᆼᮤ࣏㜭 CP4 EPSPS 㳻ⲭ൘㟣伏ѝⲴ᳤䵢ࠐ⦷˄Hammond and Jez, 2011˅DŽ䈅傼㔃᷌㺘᰾ˈCP4 EPSPS 㳻ⲭ൘ᓖ䎵䗷 75°C ᰦ࣏㜭⍫ᙗᆼޘ їཡ˄ᢰᵟᣕ 8˅DŽ 5˅CP4 EPSPS 㳻ⲭ൘ MON 87427 ⦹㊣㊭㋂ѝⲴ㺘䗮䟿ᖸվ˄3.6 μg/g 㓴㓷 勌䟽ˈ㓖ঐ⦹㊣ᙫ㳻ⲭⲴ 0.004%˅ˈഐ↔䲀ࡦҶ䙊䗷ᰕᑨ⎸䍩 MON 87427 ⦹㊣ሩ CP4 EPSPS 㳻ⲭⲴ᳤䵢≤ᒣDŽṩᦞ Hammond˄1993˅ሿ啐ᙕᙗ∂ᙗ ⹄ウ㔃᷌ NOAEL 㠣ቁѪ 572 mg/kg bwˈՠ㇇ MON 87427 ⦹㊣ѝ CP4 EPSPS 㳻ⲭሩѝഭӪ㗔㘼䀰ަ MOE Ѫ㠣ቁ 1.4 × 104 㠣 7.9 × 105ˈ˄㿱ᵜ⭣䈧Җㅜ 95 亥Ӫ㗔㟣伏ᩴޕ仾䲙䇴ՠ˅ˈᡰԕᴹ⨶⭡ᶕ᧘ᯝ伏⢙઼侢ᯉѝⲴ
XXV ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 CP4 EPSPS нՊሩӪ㊫઼ࣘ⢙䙐ᡀডᇣDŽቡ MON 87427 ⦹㊣ѝⲴ CP4 EPSPS 㳻ⲭ㘼䀰ˈањփ䟽Ѫ 50 kg ⲴӪ䴰㾱⇿ཙ⎸䍩㓖 7900 kg ˄䎵 ˅䗷㠚䓛փ䟽 150 ؽ˅Ⲵ MON 87427 ⦹㊣㊭㋂㜭䗮ࡠо Hammond˄1993 ሿ啐ᙕᙗ∂ᙗ⹄ウᡰ⭘ࡲ䟿བྷ㠤ᖃⲴ CP4 EPSPS 㳻ⲭ᳤䵢≤ᒣDŽ㘼 2015 ٬ᨀ儈ࡠ 2000 mg/kg ᒤᯠᆼᡀⲴ CP4 EPSPS ሿ啐ᙕᙗ∂ᙗ⹄ウሶ NOAEL bwˈᦞ↔䇑㇇ࠪⲴѝഭӪ㗔䙊䗷㟣伏ᩴޕ MON 87427 ⦹㊣㊭㋂㘼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ᳤䵢 MOE 儈䗮 2.76 × 106DŽ ↔ཆˈᦞ ISAAA 㔏䇑ˈᡚ→ 2014 ᒤˈޘ⨳ᴹ䎵䗷 170 䖜สഐ⢙ӗ૱㧧 ᗇҶἽᢩ߶ˈ䘉Ӌӗ૱ѝѫ㾱वᤜ CP4 EPSPSǃPAT ઼ Bt 㳻ⲭㅹ㻛ӪԜ⟏⸕ Ⲵ㳻ⲭ˄Clive, 2014b; CERA, 2015˅DŽᡰᴹ䖜สഐ⢙൘୶ъॆᓄ⭘ࡽ൷ᦞ䇱 ᦞᵳ䟽Ⲵᯩ⌅䘋㹼Ҷޘ䶒Ⲵᆹޘᙗ䇴ԧˈᒦ㓿䗷Ҷཊഭ⌅㿴ᵪᶴѕṬⲴᢰᵟᇑḕDŽ 2014 ᒤޘ⨳䖜สഐ⢙˄ѫ㾱ᱟ㙀䲔㥹ࡲ઼ᣇ㲛⢙˅ⲴἽ䶒〟ᐢ䗮 1.8 ӯޜ 产ˈঐޘ⨳ᙫ䶒〟Ⲵ 49%ˈ1996-2014 ᒤ䰤䖜สഐ⢙Ⲵ㍟〟Ἵ䶒〟ᴤ䗮 产ѻཊ˄Clive, 2014a˅DŽ㘼Ѫㅜаֻ୶ъॆⲴᙗ⣦ˈCP4 EPSPS 㳻ⲭޜӯ 18 ާᴹ൘ޘ⨳伏⢙䬮ѝ䮯ᵏᆹޘ⎸䍩ⲴশਢDŽ㠚Ӿ 1996 ᒤᣇ㥹⭈㟖བྷ䉶୶ъॆ 伏⢙ᓄ䬮ѝᆈ൘ᒦ㻛Ӫ⨳ޘἽԕᶕˈਜ਼ᴹ CP4 EPSPS 㳻ⲭⲴߌӗ૱ቡаⴤ൘ ㊫઼ࣘ⢙ᆹޘ伏⭘DŽਜ਼ᴹ CP4 EPSPS Ⲵᣇ㥹⭈㟖⢙⇿ᒤ䜭ᴹ 30 ཊњӗ૱൘ 13 њഭᇦἽ䶒〟䎵䗷 7400 зޜ产˄Dill et al., 2008˅DŽ㠚 1996 ᒤᣇ㥹⭈㟖⢙ ୶ъॆἽԕᶕˈᐢᴹ䎵䗷 1000 ӯཤࣘ⢙㻛侢லਜ਼ᴹ䖜สഐ⢙˄བྷ䜘࠶ᱟᣇ ᓧ઼⭏ӗ⦷ⲴᣕDŽаㇷ㓿䗷਼㹼䇴䇞Ⲵڕ⢙㥹⭈㟖⢙˅Ⲵ侢ᯉˈ⋑ᴹᖡ૽ࣘ ᓧᖡ૽Ⲵ᮷ڕҾ侢லਜ਼ᴹ䖜สഐ⢙˄वᤜᣇ㥹⭈㟖⢙˅侢ᯉሩࣘ⢙⭏䮯઼ޣ 㔬䘠˄Eenennaam & Young, 2014˅ҏᤷࠪˈⴞࡽ䘈⋑ᴹԫօֻᆀ㺘᰾ਜ਼ᴹ䖜⥞ ᐢ㓿ᴹ 488⨳ޘˈᓧӗ⭏н࡙ᖡ૽DŽ൘䗷৫ 20 ᒤڕสഐ⢙侢ᯉՊሩࣘ⢙⭏䮯઼ 亩⅑ޣҾ CP4 EPSPS 㳻ⲭᆹޘᙗⲴᇑḕᡆ䇴ᇑˈᗇࠪⲴ㔃䇪൷䇔Ѫ CP4 EPSPS ᓧ⋑ᴹн㢟ᖡ૽DŽڕ⢙㳻ⲭሩӪ㊫઼ࣘ ⭘শਢԕ৺ཊ⅑䘋㹼Ⲵሿ֯ޘᙗ䇴ԧǃ䮯ᵏⲴᆹޘ䶒Ⲵ伏⭘ᆹޘˈ㔬кᡰ䘠 啐ਓᴽ∂⨶ᆖ⹄ウ㔃᷌൷䇱ᇎҶ CP4 EPSPS 㳻ⲭⲴ伏⭘઼侢⭘ᆹޘᙗDŽ
৲㘳᮷⥞˖ ᓧ⣦ߥᶑḕᣕѻҼ˄2002˅ˉ㟣伏оڕ㘏ࠔ㤡, ᶘᲃݹ. 2006. ѝഭት≁㩕ޫо .1 㩕ޫ㍐ᩴޕ⣦ߥ, pp. 21-33, Ӫ≁ছ⭏ࠪ⡸⽮. 2. lj䖜สഐἽ⢙ᆹޘ䇴ԧᤷইNJ˄ѝഭߌъ䜘ˈ2010˅ 3. Bushey, D. F., et al. (2014). "Characteristics and safety assessment of intractable proteins in genetically modified crops." Regulatory Toxicology and Pharmacology 69(2): 154-170. 4. Center for Environmental Risk Assessment (CERA). 2015. GMO crop database. [accessed Jun 10, 2015] 5. Clive, J. 2014a. Global status of commercialized biotech/GM crops: 2014. ISAAA Brief No. 49. International Service for the Acquisition of Agri-Biotech Applications. Ithaca, NY. USA. 6. Clive, J. 2014b. Executive summary. Global status of commercialized biotech/GM crops: 2014. ISAAA Brief No. 49. International Service for the Acquisition of
XXVI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Agri-Biotech Applications. Ithaca, NY. USA. 7. http://www.isaaa.org/resources/publications/briefs/49/executivesummary/default.asp 8. Codex Alimentarius (2009). Foods derived from modern biotechnology. Rome, Italy, Codex Alimentarius Commission, Joint FAO/WHO Food Standards Programme, Food and Agriculture Organization of the United Nations. Second Edition. 9. Delaney, B., et al. (2008). "Evaluation of protein safety in the context of agricultural biotechnology." Food and Chemical Toxicology 46(Supplement 2): S71-S97. 10. Dill, G. M., et al. (2008). "Glyphosate-resistant crops: Adoption, use and future considerations." Pest Management Science 64: 326-331. 11. Eenennaam, A. L. v. and A. E. Young (2014). "Prevalence and impacts of genetically engineered feedstuffs on livestock populations." Journal of Animal Science 92(10): 4255-4278. 12. EFSA (2009). "Scientific opinion of the scientific committee: "Existing approaches incorporating replacement, reduction and refinement of animal testing: Applicability in food and feed risk assessment"." EFSA Journal 1052: 1-77. 13. Faustman, E. M. and G. S. Omenn (2008). Risk assessment. Casarett and Doull's Toxicology - The Basic Science of Poisons. C. D. Klassen. New York, New York, McGraw-Hill: 107-128. 14. Hammond, B. G. and A. Cockburn (2008). The safety assessment of proteins introduced into crops developed through agricultural biotechnology: A consolidated approach to meet current and future needs. Food Safety of Proteins in Agricultural Biotechnology. Boca Raton, Florida, CRC Press, Inc.: 259-288. 15. Hammond, B. and R. L. Fuchs (1998). "Safety evaluation of new varieties of food crops developed through biotechnology." Biotechnology and Safety Assessment.: 61-79. 16. Hammond, B. G. and J. M. Jez (2011). "Impact of food processing on the safety assessment for proteins introduced into biotechnology-derived soybean and corn crops." Food and Chemical Toxicology 49(4): 711-721. 17. Hammond, B., et al. (2013). "Toxicological evaluation of proteins introduced into food crops." Crit Rev Toxicol 43: 25-42. 18. ISAAA. 2014. Global biotech/GM crop plantings increase 100-fold from 1996. International Service for the Acquisition of Agri-Biotech Applications. Manila, Philippines. 19. Kuiper, H. A., et al. (2013). "New EU legislation for risk assessment of GM food: no scientific justification for mandatory animal feeding trials." Plant Biotechnology Journal 11(7): 781-784. 20. Liener, I. E. (1994). "Implications of antinutritional components in soybean foods." Critical Reviews in Food Science and Nutrition 34: 31-67. 21. Pariza, M. W. and E. A. Johnson (2001). "Evaluating the safety of microbial enzyme preparations used in food processing: update for a new century." Regul Toxicol Pharmacol 33(2): 173-186. 22. Sjoblad, R. D., et al. (1992). "Toxicological considerations for protein components of biological pesticide products." Regul. Toxicol. Pharmacol. 15(1): 3-9.
XXVII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߌъ䜘ޣҾMON 87427ᆹޘ䇴ԧ⭣䈧Ⲵഎ༽࠭ ˄2014ᒤ12ᴸ11ᰕ˅
XXVIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ҿߌъ䜘ᢩ༽ؑ࠭Ⲵ䈤᰾ޣਨޜᆏኡ䜭
䰞仈 1˖ ᙕᙗ∂ᙗ䈅傼ᵚ᤹ߌъ䜘 2031 ਧޜ-16-2013Ā䖜สഐ⭏⢙৺ަӗ૱ 伏⭘ᆹޘỰ⍻üü㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼ā䘋㹼˖ᯠ㺘䗮㳻ⲭ EPSPS ᙕᙗ∂ᙗ䈅傼Ⲵ㿲ሏᵏਚᴹ 7 ཙDŽ
䈤᰾˖ᣇ㥹⭈㟖⦹㊣ MON 87427 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭоᣇ㥹⭈㟖བྷ䉶 GTS40-3-2 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭᱟаṧˈ൷ᶕⓀҾ൏༔ߌᵶ㧼 CP4 㧼ṚDŽ GTS40-3-2 ᱟޘ⨳ㅜањ㻛ᢩ߶୶ъॆἽⲴᣇ㥹⭈㟖⢙ˈҾ 1996 ᒤ൘㖾ഭ ᔰ୶ъॆἽˈ䲿ਾࠐᒤⲴἽ㤳ത䗵䙏ᢙኅࡠे㖾৺ই㖾Ⲵ࣐བྷǃ䱯ṩ ᔧǃᐤ㾯ǃᐤ൝ㅹഭᇦ઼ൠ४DŽࡠⴞࡽѪ→ˈᐢ㓿୶ъॆᓄ⭘Ⲵ䖜 cp4 epsps สഐᣇ㥹⭈㟖⢙ᴹ 6 ˈवᤜ㤌㬯ǃ⋩㨌ǃ⦹㊣ǃỹ㣡ǃབྷ䉶઼⭌㨌˄㿱л 㺘˅DŽ䘉Ӌ㺘䗮 CP4 EPSPS 㳻ⲭⲴᣇ㥹⭈㟖⢙൘ޘ⨳ᒯ⌋Ἵ઼⎸䍩ˈᡀѪ ᓄ⭘䶒〟ᴰབྷⲴа㊫䖜สഐ⢙DŽ
ᐢ㓿ᢩ߶ᒦ൘ޘ⨳ᒯ⌋ᓄ⭘Ⲵਜ਼ CP4 EPSPS 㳻ⲭⲴ䖜สഐ⢙ ᣇ㥹⭈㟖⢙ FDA ᢩ߶ᰦ䰤 USDA ᢩ߶ᰦ䰤 㳻ⲭլᙗ* ᣇ㥹⭈㟖བྷ䉶 GTS40-3-2** 1996 1994 100% ᣇ㥹⭈㟖ỹ㣡 MON 1445** 1996 1995 100% ᣇ㥹⭈㟖⋩㨌 GT73** 1995 1999 100% ᣇ㥹⭈㟖⦹㊣ NK603** 2000 2000 100/99.9%*** ᣇ㥹⭈㟖 Flex ỹ㣡 MON 88913** 2005 2004 100% ᣇ㥹⭈㟖㤌㬯 J101 ઼ J163 2004 2005 100% ᣇ㥹⭈㟖⭌㨌 H7-1** 2004 2005 100% ᣇ㲛ᣇ㥹⭈㟖⦹㊣ MON 88017** 2005 2005 100% ᣇ㥹⭈㟖儈ӗབྷ䉶 MON 89788** 2006 2007 100% 儈⋩䞨ᣇ㥹⭈㟖བྷ䉶 MON 87705 2011 2011 100% ᣇ㥹⭈㟖⦹㊣ MON 87427 2012 2013 100% ᣇ㥹⭈㟖⋩㨌 MON 88302 2012 2013 100%
* ԕᣇ㥹⭈㟖བྷ䉶 GTS40-3-2 Ⲵ CP4 EPSPS 㳻ⲭѪ৲➗ ** ᐢ㓿㧧ᗇѝഭᢩ߶Ⲵ䘋ਓᆹޘ䇱Җ˄⭘࣐ᐕᯉ˅ *** NK603 ਜ਼ᴹєњᯠ㺘䗮㳻ⲭˈCP4 EPSPS ઼ CP4 EPSPS L214Pˈє㘵ਚᴹањ≘ ส䞨Ⲵᐞ࡛
⭡Ҿ㺘䗮Ⲵ㳻ⲭ਼ˈᒦфᐢ䇱ᇎἽ⢙ᶕⓀ CP4 EPSPS 㳻ⲭоབྷ㛐ᵶ㧼ᶕ Ⓚ㳻ⲭⲴㅹ਼ᙗˈ䘉Ӌ䖜สഐ⢙ᆹޘᙗ䇴ԧᡰ⭘Ⲵ㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼ᣕ ൷䟷⭘ Hammond Ҿ 1993 ᆼᡀⲴ CP4 EPSPS 㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼DŽHammond ˄1993˅൘䈅傼ѝⲴѤᒺ㿲ሏᤱ㔝Ҷ 7 ཙˈ⭡Ҿ⋑ᴹ㿲ሏࡠн㢟৽ᓄˈㅜ 8 ཙ༴ ↫ሿ啐ᒦ䘋㹼⯵⨶ᆖ㿲ሏˈ㔃᷌ҏᵚਁ⧠ᔲᑨ˄㿱ᵜ⭣䈧Җлᢰᵟᣕ 10˅DŽ
XXIX ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㳻ⲭ∂㍐а㡜ᱟ䙊䗷ᙕᙗ⭘ᵪࡦӗ⭏∂ᙗˈ᧕䀖ਾࠐሿᰦ㠣ࠐཙቡՊ㺘 ⧠ࠪн䘲৽ᓄˈа㡜нՊ䎵䗷аઘDŽᐢ⸕ⲴἽ⢙㳻ⲭ∂㍐䜭ᱟ൘ᮠሿᰦӗ⭏∂ ᙗˈ∄ྲ˖㬆哫∂㍐ᱟ 3-24 ሿᰦǃ㓒䉶∂㍐ᱟ 6-8 ሿᰦǃἽ⢙ࠍ䳶㍐ A ѪሿҾ 3 ሿᰦDŽ㘼ᐢ⸕Ⲵ㓶㧼㳻ⲭ∂㍐਼ṧՊ൘ᖸ⸝ᰦ䰤㺘⧠ࠪ∂ᙗ⯷⣦ˈྲ䴽ҡ⯵∂ Ѫ 1-5 ཙǃ㪑㨴⨳㧼㛐∂㍐ B Ѫ 1-6 ሿᰦǃ㚹∂㧼∂㍐Ѫ 18-36 ሿᰦDŽHammond ˄1993˅䘋㹼Ⲵ CP4 EPSPS ሿ啐㓿ਓᙕᙗ∂ᙗ䈅傼ѝˈ♼㛳ਾ⋑ᴹࠪ⧠н㢟৽ ᓄˈ൘↔สкѤᒺ㿲ሏᤱ㔝Ҷ 7 ཙDŽѤᒺ㿲ሏ㔃ᶏਾˈབྷփ䀓ࢆҏᵚ㿲ሏࡠ㜿 ಘᔲᑨDŽHammond˄1993˅⹄ウѝ 7 ཙ㿲ሏᵏ⏥ⴆҶབྷཊᐢ⸕㳻ⲭ∂㍐ਁᥕ∂ ᙗⲴᴹ᭸ᰦ䰤ˈণ֯䘉ᰦ㓸→䈅傼ˈҏᓄ䈕ਟԕ⺞䇔 CP4 EPSPS 㳻ⲭ൘䈅傼ࡲ 䟿лнާᴹ∂ᙗDŽ ൘ Hammond˄1993˅Ⲵ䈅傼ਾˈᆏኡ䜭ޜਨ䘈䪸ሩᣇ㥹⭈㟖⦹㊣ NK 603 ѝⲴ CP4 EPSPS 㳻ⲭ䘋㹼Ҷਖཆа亩㓿ਓᙕᙗ∂ᙗ䈅傼˄Bonnette, 2002˅DŽ Bonnette˄2002˅䈅傼ѝⲴ CP4 EPSPS 㳻ⲭо Hammond˄1993˅ᡰ⭘㳻ⲭӵ ᐞањ≘ส䞨ˈ਼Ⓚᙗ䗮ࡠ 99.9%˄㿱к㺘˅DŽBonnette˄2002˅൘䈅傼ѝ䟷⭘ Ⲵ㳻ⲭ♼㛳ࡲ䟿Ѫ 817 mg/kgˈѤᒺ㿲ሏᵏѪ 14 ཙˈ⋑ᴹ㿲ሏࡠ CP4 EPSPS ൘ 䈅傼ᴰ儈ࡲ䟿≤ᒣлާᴹн㢟᭸ᓄˈ䈅傼ᡰᗇ㔃䇪о Hammond˄1993˅㔃䇪а ṧDŽBonnette˄2002˅Ⲵ䈅傼ᣕᐢ䲿ᣇ㥹⭈㟖⦹㊣ NK 603 Ⲵᆹޘ䇴ԧ⭣䈧䙂 Ӕࡠޘ⨳ഭᇦⲴ⌅㿴ⴁ㇑ᵪᶴˈᒦ㻛ᇑḕ઼ᢩ߶DŽ ਨሩ MON 87427 䖜สഐޜ䇶᮷Ԧ઼∂⨶ᆖ⹄ウᜟֻˈᆏኡ䜭ޡ 䚥ᗚ Codex ∄㊣ѝᯠ㺘䗮㳻ⲭ CP4 EPSPS 䘋㹼Ҷ˖㔃ᶴ⍫ᙗ࠶᷀˄਼ⓀᙗⲴ⭏⢙ؑᆖ⦹ ⭘শਢ˅ǃփཆ⍻䈅˄✝っᇊᙗ઼ SGF ⎸ॆっᇊ֯ޘሩ˅ǃ⍱㹼⯵ᆖ࠶᷀˄ᆹ ᙗ˅ˈԕ৺ࣘ⢙⭏⍻˄ԕ㓟ॆ㳻ⲭ䘋㹼ሿ啐ᙕᙗ∂ᙗ⹄ウ˅DŽ䘉Ӌ࠶᷀䍴ᯉҏ㺘 ᰾ CP4 EPSPS нՊ㺘⧠ࠪᔦ䘏∂ᙗ˄䈖㿱ᵜ⭣䈧Җѝㅜ 3.3 䜘࠶˅˖ ˗⭏⢙ؑᆖ࠶᷀㺘᰾ˈCP4 EPSPS оᐢ⸕Ⲵ∂㍐㳻ⲭнާᴹ਼Ⓚᙗ˅6 7˅CP4 EPSPS оӪ㊫઼ࣘ⢙ᰕᑨ伏⭘Ⲵᒦᐢ䇱᰾ᱟᆹޘⲴἽ⢙㳻ⲭ˄ྲἽ ⢙Ⓚ EPSPS 㳻ⲭ˅ᴹᖸ儈਼Ⓚᙗˈ㘼䘉Ӌ㳻ⲭ൷ާᴹ䮯ᵏᆹޘ伏⭘শਢ˗ 8˅փཆ⎸ॆ䈅傼㺘᰾ˈCP4 EPSPS 㳻ⲭ൘⁑ᤏ㛳⏢˄SGF˅ѝՊ㻛䗵䙏⎸ ॆᦹˈнާᴹ㌫㔏ᙗ᳤䵢䰞仈DŽ 9˅CP4 EPSPS 㳻ⲭሩ✝нっᇊˈ㘼ᰕᑨ㟣伏ѝ⦹㊣䙊ᑨ䴰㾱✩ࡦˈ߿ቁҶ ާᴹᆼᮤ࣏㜭 CP4 EPSPS 㳻ⲭ൘㟣伏ѝⲴ᳤䵢ࠐ⦷˄Hammond and Jez, 2011˅DŽ䈅傼㔃᷌㺘᰾ˈCP4 EPSPS 㳻ⲭ൘ᓖ䎵䗷 75°C ᰦ࣏㜭⍫ᙗᆼޘ їཡDŽ 㘳㲁ࡠབྷཊᮠ㳻ⲭ䜭нާᴹ∂ᙗǃCP4 EPSPS 㳻ⲭާᴹᆹޘ⎸䍩শਢԕ৺ᣇ MOE˅䜭ᖸབྷ˄MON 87427 ⦹㊣ѝ CP4 EPSPS˄㥹⭈㟖⢙ѝ䈕㳻ⲭⲴ᳤䵢䲀٬ 㳻ⲭሩѝഭӪ㗔㘼䀰ަ MOE Ѫ㠣ቁ 1.4 × 104 㠣 7.9 × 105ˈ㿱ᵜ⭣䈧Җㅜ 102-104 亥Ӫ㗔㟣伏ᩴޕ仾䲙䇴ՠ˅ˈᡰԕᴹ⨶⭡ᶕ᧘ᯝ伏⢙઼侢ᯉѝⲴ CP4 EPSPS н ՊሩӪ㊫઼ࣘ⢙䙐ᡀডᇣDŽቡ MON 87427 ⦹㊣ѝⲴ CP4 EPSPS 㳻ⲭ㘼䀰ˈањ փ䟽Ѫ 50 kg ⲴӪ䴰㾱⇿ཙ⎸䍩㓖 7900 kg˄䎵䗷㠚䓛փ䟽 150 ؽ˅Ⲵ MON 87427
XXX ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⦹㊣㊭㋂㜭䗮ࡠо Hammond˄1993˅ሿ啐ᙕᙗ∂ᙗ⹄ウᡰ⭘ࡲ䟿བྷ㠤ᖃⲴ CP4 EPSPS 㳻ⲭ᳤䵢≤ᒣDŽ ↔ཆˈCP4 EPSPS 㳻ⲭާᴹ൘ޘ⨳伏⢙䬮ѝ䮯ᵏᆹޘ⎸䍩ⲴশਢDŽ㠚Ӿ 1996 ᒤᣇ㥹⭈㟖བྷ䉶୶ъॆἽԕᶕˈਜ਼ᴹ CP4 EPSPS 㳻ⲭⲴߌӗ૱ቡаⴤ൘ޘ⨳ ⢙伏⭘DŽਜ਼ᴹ CP4 EPSPS Ⲵᣇ㥹⭈㟖ޘ伏⢙ᓄ䬮ѝᆈ൘ᒦ㻛Ӫ㊫઼ࣘ⢙ᆹ ⇿ᒤ䜭ᴹ 30 ཊњӗ૱൘ 13 њഭᇦἽ䶒〟䎵䗷 7400 зޜ产˄Dill et al., 2008˅DŽ 㠚 1996 ᒤᣇ㥹⭈㟖⢙୶ъॆἽԕᶕˈᐢᴹ䎵䗷 1000 ӯཤࣘ⢙㻛侢லਜ਼ᴹ䖜 ᓧ઼⭏ӗ⦷ⲴᣕDŽڕ⢙สഐ⢙˄བྷ䜘࠶ᱟᣇ㥹⭈㟖⢙˅Ⲵ侢ᯉˈ⋑ᴹᖡ૽ࣘ аㇷ㓿䗷਼㹼䇴䇞ⲴޣҾ侢லਜ਼ᴹ䖜สഐ⢙˄वᤜᣇ㥹⭈㟖⢙˅侢ᯉሩࣘ⢙ ᓧᖡ૽Ⲵ᮷⥞㔬䘠˄Eenennaam & Young, 2014˅ҏᤷࠪˈⴞࡽ䘈⋑ᴹڕ⭏䮯઼ ᓧӗ⭏н࡙ᖡ૽DŽ൘䗷৫ڕԫօֻᆀ㺘᰾ਜ਼ᴹ䖜สഐ⢙侢ᯉՊሩࣘ⢙⭏䮯઼ 20 ᒤˈޘ⨳ᐢ㓿ᴹ 488 亩⅑ޣҾ CP4 EPSPS 㳻ⲭᆹޘᙗⲴᇑḕᡆ䇴ᇑˈᗇࠪⲴ ᓧ⋑ᴹн㢟ᖡ૽DŽڕ⢙㔃䇪൷䇔Ѫ CP4 EPSPS 㳻ⲭሩӪ㊫઼ࣘ
৲㘳᮷⥞ Codex Alimentarius. 2003. Guideline for the conduct of food safety assessment of foods derived from recombinant-DNA plants. CAC/GL 45-2003. Codex Alimentarius Comission, Joint FAO/WHO Food Standards Programme, Food and Agriculture Organisation, Rome, Italy Codex Alimentarius. 2009. Foods derived from modern biotechnology. Second Edition, World Health Organization, Food and Agriculture Organization of the United Nations, Rome, Italy. Dill, G.M., C.A. CaJacob and S.R. Padgette. 2008. Glyphosate-resistant crops: Adoption, use and future considerations. Pest Management Science 64:326-331. Van Eenennaam, A.L. 2014. Prevalence and impacts of genetically engineered feedstuffs on livestock populations. Journal of Animal Science 92:4255-4278.
XXXI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䰞仈 2˖ ᵚᨀᯠ㺘䗮㳻ⲭ EPSPS 䞦⍫ᙗǃ䞦⍫ᙗᖡ૽ഐ㍐˄ྲ pHǃᓖǃ ᆀᕪᓖ˅ǃᓅ⢙⢩ᔲᙗ઼৽ᓄӗ⢙ㅹ䍴ᯉDŽ
䈤᰾˖5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦˄EPSPS˅ᒯ⌋ᆈ൘ҾἽ⢙઼ᗞ⭏⢙ փˈᱟ㧭㥹䞨䙄ᖴѝⲴањޣ䭞䞦ˈ㘼㧭㥹䞨䙄ᖴ䍏䍓൘Ἵ⢙઼ᗞ⭏⢙փ ⢙ᨀ㣣俉⧟ˈ⭘Ҿ㣣俉᯿≘ส䞨˄㤟щ≘䞨ǃ䞚≘䞨ǃ㢢≘䞨˅઼䇨ཊ⅑㓗 ˄ྲഋ≒ਦ䞨ǃ⌋䞼ǃ㔤⭏㍐ Kǃ㣡䶂㍐ǃ㊫哴䞞ǃᵘ䍘㍐ㅹ˅Ⲵ⭏⢙ਸᡀ ˄Herrmann and Weaver 1999; Maeda and Dudareva 2012˅DŽ䈕䙊䐟൘Ἵ⢙փ䶎 ᑨ䟽㾱ˈἽ⢙փ㠣ቁ 35%Ⲵᒢ⢙䍘ᶕⓀҾ㧭㥹䞨䙄ᖴⲴӗ⢙˄Franz et al. 1997˅DŽ лമᡰ⽪ˈEPSPS 䞦⭘Ҿ㧭㥹䞨䙄ᖴѝⲴㅜ 6 ↕ˈ䍏䍓ۜॆሶ⼧䞨✟䞷ྲ ᔿщ䞞䞨˄PEP˅кⲴ✟䞷ᔿщ䞞䞠ส䖜〫ࡠ㧭㥹䞨-3-⼧䞨˄S3P˅кˈ৽ᓄ⭏ 2- ᡀ 5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨˄EPSP˅ˈ਼ᰦ䟺᭮ࠪᰐᵪ⼧䞨˄HPO4 ˅DŽ ˄Alibhai and Stallings, 2001˅DŽ㘼ߌ䗮®䲔㥹ࡲѝⲴᴹ᭸ᡀ࠶㥹⭈㟖˄glyphosate˅ ᱟ PEP Ⲵㄎҹᙗᣁࡦ⢙ˈ㥹⭈㟖Ⲵᆈ൘ਟ䱫→ EPSPS 䞦ሩ PEP Ⲵ࡙⭘ˈ䱫ᯝ㣣 䭞ԓ䉒ӗ⢙ˈӾ㘼ਁᥕ䲔㥹⭘DŽޣ俉⧟Ⲵਸᡀˈ֯ᗇἽ⢙㕪ѿབྷ䟿⭏䮯ᡰ䴰Ⲵ 儈ㅹ⭏⢙ྲପңࣘ⢙փнᆈ൘㧭㥹䞨䙄ᖴˈਚ㜭Ӿ伏⢙ѝ㧧ਆ㣣俉᯿≘ส䞨˗ ⭡Ҿ㠚䓛н㜭ਸᡀ EPSPS 䞦ˈପңࣘ⢙ሩ㥹⭈㟖нᝏDŽ
PEP
Inhibitor:
Shikimate-3-phosphate Glyphosate 5-Enolpyruvyl-shikimate-3-phosphate S3P EPSP
CP4 EPSPS ᴰࡍᱟӾ൏༔ѝᑨ㿱Ⲵߌᵶ㧼˄Agrobacterium˅CP4 㧼Ṛѝ࠶ ᗇࡠⲴDŽ⭡Ҿަۜॆս⛩Ⲵ㔃ᶴ⢩ᖱˈCP4 EPSPS ሩ㥹⭈㟖Ⲵᣁࡦާᴹཙ❦Ⲵн ᝏᙗ˄Funke, et al. 2006˅DŽᣇ㥹⭈㟖⢙ѝⲴ CP4 EPSPS 䙊ᑨ൘ N-ᵛㄟᑖᴹ а⇥ਦ㔯փ䖜䘀㛭ˈ⭘Ҿᕅሬ CP4 EPSPS ᇊսࡠਦ㔯փкˈ䘉ᱟⓀᙗἽ⢙ EPSPS 䞦൘㓶㜎Ⲵս㖞DŽ CP4 EPSPS ᱟ⭡ 455 њ≘ส䞨㓴ᡀⲴঅ䬮ཊ㛭ˈ⨶䇪࠶ᆀ䟿Ѫ 47.6 kDaDŽӾ ᒿࡇкⴻˈCP4 EPSPS оἽ⢙ᶕⓀⲴ EPSPS ާᴹ 25-30%Ⲵլᙗˈо㓶㧼ᶕⓀ Ⲵ EPSPS ާᴹ 50-90%ⲴլᙗDŽ㲭❦൘㳻ⲭ䍘а㓗㔃ᶴк㊫ EPSPS Ⲵ≘ส䞨 ᒿࡇᒦнॱ࠶լˈն EPSPS 㳻ⲭᇦ᯿൘䞦⍫ᙗѝᗳ઼й㓗㔃ᶴⲴޣ䭞ս⛩к ᆸˈഐ↔ⴞࡽᐢ⸕ⲴᡰᴹᶕⓀ EPSPS 䞦˄वᤜ CP4 EPSPS˅൘й؍Ⲵᒿࡇ䶎ᑨ 㓗ᤃᢁ㔃ᶴк儈ᓖа㠤˄Hammond, et al. 2013˅˄മ 2˅DŽӾᇊѹкᶕ䈤ˈ਼Ⓚ㳻 ⲭᴹլⲴҼ㓗㔃ᶴ઼й㓗ᶴ䊑ˈӾ㘼ާᴹլⲴ࣏㜭˄Pearson, 2000˅DŽ CP4 EPSPS ԕঅփᖒᔿᢗ㹼ۜॆ࣏㜭ˈн䴰㾱䖵䞦ᡆ⢩↺䠁ᆀ䖵ࣙDŽ
XXXII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ѫᵚоᓅ⢙㔃ਸⲴ CP4 EPSPSחCP4 EPSPS Ⲵй㓗Ღփ㔃ᶴྲлമᡰ⽪ˈA മᐖ Ѫоᓅ⢙ S3P 㔃ਸਾⲴ䰝ਸ⣦ᘱⲴ䞦DŽоަᆳח䞦ˈԕᔰ᭮⣦ᘱⲴᶴ䊑ᆈ൘˗ਣ EPSPS аṧˈCP4 EPSPS 䞦⭡є䜘࠶ॺ⨳⣦㔃ᶴฏ䙊䗷䬠䬮४䘎᧕ˈ⇿њॺ⨳ 㔃ᶴฏ൷⭡ཊњᒣ㹼Ⲵ-㷪㓴ᡀˈ䞦ۜॆ⍫ᙗս⛩սҾєњॺ⨳⣦㔃ᶴฏѻ⣦ ⢙䰤ˈ⍫ᙗս⛩䱴䘁ਜ਼ᴹ䖳ཊᑖ⭥㦧Ⲵ≘ส䞨↻สˈׯҾоᓅ⢙ӂ⭘DŽоᓅ S3P Ⲵ㔃ਸ䈡ሬ䞦Ⲵᶴ䊑ਁ⭏⛸ਈॆˈᵜĀᢃᔰā⣦ᘱⲴєњॺ⨳⣦㔃ᶴ ฏ䰝ਸˈׯҾ䞦઼ PEP Ⲵ㔃ਸ˄ྲ B മᡰ⽪˅DŽCP4 EPSPS ሩ㥹⭈㟖Ⲵ㙀ਇ↓ᱟ ⭡Ҿަ⢩↺Ⲵࠐօᆖᶴ䙐⢩⛩˄Funke, et al. 2006˅DŽA മѝ₉㢢⧟⣦㔃ᶴᱟ䶐䘁 ᆸ↻ส Glu-354؍ᴹⲴ儈ᓖޡC-ᵛㄟⲴㅜ 347-358 ս↻สˈवᤜ EPSPS 䞦ᇦ᯿ᡰ ઼ Arg-357ˈо PEP/㥹⭈㟖Ⲵ㔃ਸᴹޣDŽ⢩࡛䟽㾱Ⲵᱟ CP4 EPSPS ㅜ 100 սк 䬮䱫→Ҷ㥹⭈㟖о䞦Ⲵ㔃חⲴщ≘䞨↻ส˄㿱 B മѝ A100˅DŽщ≘䞨кⲴ⭢ส ਸ˄Funke, et al. 2006˅DŽӪѪሶㅜ 100 սкⲴщ≘䞨ケਈᡀ⭈≘䞨ਾˈケਈⲴ CP4 EPSPS 䞦ሩ㥹⭈㟖ਈᗇᝏDŽѻࡽҏᴹ⹄ウ㺘᰾ˈབྷ㛐ᵶ㧼 EPSPS ѝᓄ ս⛩ྲ᷌Ӿ⭈≘䞨ケਈᡀщ≘䞨˄Gly96Ala˅ˈ㜭ཏབྷབྷ䱽վަሩ㥹⭈㟖Ⲵᝏ ᙗˈն਼ᰦо PEP ⲴӢ઼ᙗҏབྷབྷл䱽˄Barry et al. 1992; Padgette et al. 1996˅DŽ ᤱ؍ CP4 EPSPS 䞦Ⲵ⤜⢩ᶴ䙐֯ᗇަሩ㥹⭈㟖нᝏˈն਼ᰦሩཙ❦ᓅ⢙ PEP ᖸ儈ⲴӢ઼ᙗDŽ EPSPS 䞦˄वᤜ CP4 EPSPS˅ާᴹ儈ᓖⲴᓅ⢙⢩ᔲᙗDŽⴞࡽᐢ⸕ሩ EPSPS ਈॆDŽ׳䞦ާᴹ⭏⢙ᆖѹⲴᓅ⢙ӵѪ S3P ઼ PEPDŽ㥹⭈㟖н㜭ਁ⭏ EPSPS 䞦 㧭㥹䞨оབྷ㛐ᵶ㧼ᶕⓀ EPSPS 䞦Ⲵ㔃ਸ㜭࣋䶎ᑨᕡˈо S3P ∄ˈ㧭㥹䞨䴰㾱 ˈ䶎ᑨ儈⎃ᓖ˄mM 㓗˅ᴹਟ㜭㻛 EPSPS 䞦ۜॆ˄Gruys, et al. 1992˅DŽ⹄ウ㺘᰾ Ἵ⢙փཙ❦ᆈ൘ S3P ㊫լ⢙ྲ⭢ส㧭㥹䞨ǃཾቬ䞨ǃҼ≒㧭㥹䞨䜭нᱟ EPSPS Ⲵᓅ⢙˄Franz, et al. 1997˅DŽ ٬ǃᓖ઼ᆀᕪᓖㅹഐ㍐ᖡ૽DŽ CP4 EPSPS 㳻ⲭⲴ䞦⍫ᙗ઼っᇊᙗਇ pH ٬䗷儈ᡆ䗷վ䜭Պ䱽վ䞦⍫ᙗˈpH ሿҾ 5.5 ᰦ ᴰ䘲䞦⍫ pH Ѫ 9-9.5ˈpH CP4 EPSPS 䞦⍫ࠐѾᆼޘїཡˈн䗷⭡ pH ᕅ䎧Ⲵ䞦⍫ਈॆᒦ䶎нਟ䘶˄Padgette et al. 1993˅DŽCP4 EPSPS ሩ✝нっᇊˈᓖ䎵䗷 75°C ᰦˈCP4 EPSPS Ⲵ࣏㜭⍫ ᙗণ䱽㠣Ự⍻䲀ԕлˈն࣐✝ᒦнᖡ૽㳻ⲭа㓗㔃ᶴⲴᆼᮤᙗ˄䈖㿱ᵜ⭣䈧Җѝ ㅜ 3.3 䜘࠶˅DŽ䘉ㅖਸ✝ࣘ࣋ᆖ⌅ࡉˈণᓖॷ儈Պ֯㳻ⲭ儈㓗㔃ᶴਈᙗˈᕅ䎧 䞦⍫ᙗїཡDŽCP4 EPSPS ሩ✝нっᇊⲴ⢩ᙗ㺘᰾ˈ൘ᐕъ࣐ᐕᶑԦлˈᴰ㓸Ⲵ䖜 สഐ⦹㊣ӗ૱ѝнཚਟ㜭ᆈ൘ާᴹ࣏㜭⍫ᙗⲴ CP4 EPSPS 㳻ⲭ˄Hammond and Jez 2011˅DŽоᡰᴹިරⲴ㳻ⲭ䞦㊫аṧˈCP4 EPSPS Ⲵۜॆ⍫ᙗ䘈ਇ৽ᓄⓦ⏢ѝ ᆀᕪᓖⲴᖡ૽DŽ↓ᑨⲴ䍞ᆈ㕃ߢ⏢˄1mMKCl˅ᶑԦлˈCP4 EPSPS Ⲵ⢩ᔲ ⍫ᙗᴰ儈㜭ཏ䗮ࡠ 5.5 U/mgDŽCP4 EPSPS Ⲵ⢩ᔲ⍫ᙗ䲿⵰ᆀᕪᓖ໎བྷ㘼ॷ儈ˈ 㠣 50 mM KCl ᰦˈ䞦⍫ᙗ㜭ཏ໎࣐ 2-2.6 ؽ˄Padgette et al. 1993˅DŽ
XXXIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ৲㘳᮷⥞ Alibhai, M.F. and W.C. Stallings. 2001. Closing down on glyphosate inhibition - With a new structure for drug discovery. Proceedings of the National Academy of Sciences of the United States of America 98:2944-2946. Barry, G., G. Kishore, S. Padgette, M. Taylor, K. Kolacz, M. Weldon, D. Re, D. Eichholtz, K. Fincher and L. Hallas. 1992. Inhibitors of Amino Acid Biosynthesis: Strategies for Imparting Glyphosate Tolerance to Crop Plants. Pages 139-145 in Biosynthesis and molecular Regulation of Amino Acids in Plants B.K. Singh, H.E. Flores, and Shannon (eds.). American Soceity of Plant Physiologists. Franz, J.E., M.K. Mao and J.A. Sikorski. 1997. Glyphosate's Molecular Mode of Action. Glyphosate A Unique Global herbicide. American Chemical Society, Washington, DC.
XXXIV ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Funke, T., H. Han, M.L. Healy-Fried, M. Fischer and E. Schonbrunn. 2006. Molecular basis for the herbicide resistance of Roundup Ready crops. Proc Natl Acad Sci U S A 103:13010-13015. Gruys, K.J., M.C. Walker and J.A. Sikorski. 1992. Substrate synergism and the steady-state kinetic reaction mechanism for EPSP synthase from Escherichia coli.Biochemistry 31:5534-5544. Hammond, B., J. Kough, C. Herouet-Guicheney, J.M. Jez. 2013. Toxicological evaluation of proteins introduced into food crops. Crit Rev Toxicol 43 Suppl 2:25-42. Hammond, B.G. and J.M. Jez. 2011. Impact of food processing on the safety assessment for proteins introduced into biotechnology-derived soybean and corn crops. Food Chem Toxicol 49:711-721. Herrmann, K.M. and L.M. Weaver. 1999. The Shikimate Pathway. Annual revel of Plant Physiology and Plant Molecular Biology 50:473-503. Kishore, G.M. and D.M. Shah. 1988. Amino acid biosynthesis inhibitors as herbicides. Annu Rev Biochem 57:627-663. Maeda, H. and N. Dudareva. 2012. The shikimate pathway and aromatic amino Acid biosynthesis in plants. Annu Rev Plant Biol 63:73-105. Padgette, S.R., G.F. Barry, D.B. Re, D.A. Eichholtz, M. Weldon, K. Kolacz and G.M. Kishore. 1993. Purification, Cloning, and Characterization of a Highly Glyphosate-tolerant 5-Enolpyruvylshikimate-3-phosphate Synthase from Agrobacterium sp. strain CP4. Monsanto Technical Report (St. Louis) MSL-12738. Padgette, S.R., D.B. Re, G.F. Barry, D.E. Eichholtz, X. Delannay, R.L. Fuchs, G.M. Kishore and R.T. Fraley. 1996. New weed control opportunities: Development of soybeans with a Roundup ReadyTM gene. Pages 53-84 in Herbicide-Resistant Crops: Agricultural, Environmental, Economic, Regulatory, and Technical Aspects. S.O. Duke (ed.). CRC Press, Boca Raton, Florida. Pearson, W.R. 2000. Flexible sequence similarity searching with the FASTA3 program package. Methods in Molecular Biology 132:185-219.
XXXV ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ӗ૱ѝ䲔㥹ࡲ㥹⭈㟖ޣ䰞仈 3˖ᵚᨀ⦹㊣ᆀǃ⦹㊣ǃ⦹㊣⋩ǃ⦹㊣㊹ㅹ Ⲵ↻⮉䟿ᮠᦞDŽ 䈤᰾˖㥹⭈㟖ᱟа䶎䘹ᤙᙗᒯ䉡䲔㥹ࡲˈ㜭ཏᣁࡦἽ⢙Ⓚ 5-✟䞷ᔿ㧭㥹䞨щ 䞞䞠-3-⼧䞨ਸ䞦˄EPSPS˅Ⲵ⍫ᙗˈӾ㘼䱫ᯝἽ⢙⭏䮯ᡰᗵ䴰Ⲵ㣣俉᯿≘ส䞨Ⲵ ⭏⢙ਸᡀˈሬ㠤Ἵ⢙↫ӑˈ䗮ࡠ䲔㥹ⴞⲴDŽᣇ㥹⭈㟖㊫䖜สഐ⢙࡙⭘Ҷаሩ 㥹⭈㟖нᝏⲴ EPSPS 䞦˄⭡ҾᶕⓀҾ൏༔ߌᵶ㧼 CP4 㧼ṚˈભѪ CP4 EPSPS˅ˈ Ⓚᙗ EPSPS Ⲵ࣏㜭ˈ֯⢙⭏䮯нਇ㥹⭈㟖ᣁࡦDŽگ൘௧ᯭ㥹⭈㟖ਾˈ㜭ཏԓ ൘ᯠⲴᣇ䲔㥹ࡲᙗ⣦୶ъॆἽѻࡽˈՊ᤹➗㢟ྭߌъ㿴㤳˄Good Agricultural Practices ˈGAP˅ḷ߶䘋㹼⭠䰤䈅傼ˈ᤹➗᧘㦀ࡲ䟿઼ᯩ⌅൘ ⢙кⴤ᧕௧ᯭ䲔㥹ࡲˈ࠶᷀⺞ᇊⴤ᧕᭦㧧Ⲵߌӗ૱৺ަ࣐ᐕ㓴࠶ѝⲴ䲔㥹ࡲ↻ ⮉≤ᒣDŽ䲔㥹ࡲ൘㊭㋂н਼㓴࠶ѝ↻⮉≤ᒣਟ㜭ᆈ൘ᐞᔲˈ൘ḀӋ㓴࠶ѝሩ ᇼ䳶ˈ㘼൘ަᆳ㓴࠶ѝሩ䖳ቁDŽ࣐ᐕ㓴࠶ѝⲴሩ↻⮉⎃㕙≤ᒣ⭘⎃㕙㌫ᮠ ˄concentration factors, CF˅㺘⽪ˈ䇑㇇ޜᔿྲл˖CF=࣐ᐕ㓴࠶↻⮉≤ᒣ/ᵚ ࣐ᐕ㊭㋂↻⮉≤ᒣDŽ 䪸ሩ俆њᣇ⢩ᇊ䲔㥹ࡲᙗ⣦ᔰਁⲴ↻⮉ᮠᦞਟԕѪ䈕㊫ӗ૱Ⲵสᮠ ᦞˈਾ㔝ᔰਁⲴᣇ਼䲔㥹ࡲⲴӗ૱ਚ㾱ᕅޕ㳻ⲭⲴ⭘ᵪࡦ਼ˈ∄ྲ䜭ᱟ ᣇ㥹⭈㟖Ⲵ EPSPS 㳻ⲭˈᒦ᤹➗਼ḷㆮ᧘㦀Ⲵ௧ᯭࡲ䟿઼ᯩ⌅֯⭘䲔ޕᕅ 㥹ࡲˈᴰࡍᔰਁⲴ↻⮉สᮠᦞਟ⭘Ҿᯠ䖜ॆһԦӗ૱Ⲵ↻⮉䟿䇴ՠDŽሩҾᣇ 䲔㥹ࡲ⦹㊣ᶕ䈤ˈสҾㅜањᣇ㥹⭈㟖⦹㊣ GA21 ᔰਁⲴ↻⮉ᮠᦞवᤜ⦹㊣㊭ ㋂઼㊭㋂࣐ᐕ㓴࠶⦹㊣⋩઼⦹㊣㊹˄㿱л㺘˅ˈ䘉Ӌ㓴࠶สᵜ⏥ⴆҶ⦹㊣⭘ ࣐ᐕᯉⲴ䘋ਓӗ૱DŽᆀ઼㊭㋂൘ᾲᘥкᱟਟԕӂᦒⲴˈն⦹㊣ᵲӔᆀⲴ ⴞⲴᱟ⭘ᶕἽˈ䙊ᑨнՊ⭘ҾӪ㊫⎸䍩ˈ亴䇑⦹㊣ᆀⲴ↻⮉䟿亴䇑઼㊭㋂ լDŽ䘉Ӌ↻⮉สᮠᦞ䘲⭘Ҿᣇ㥹⭈㟖⦹㊣ MON 87427×NK603 ǃ MON 87427 × MON 88017 ㅹ਼㊫ӗ૱DŽ MON 87427 Ⲵ䳴ᙗಘᇈѝ CP4 EPSPS 㳻ⲭ㺘䗮䟿ᶱվˈн㜭㙀ਇ㥹⭈㟖 䲔㥹ࡲDŽ൘⦹㊣ᵲӔࡦ䗷〻ѝˈ⭘ਜ਼ᴹ MON 87427 䖜ॆһԦⲴ⦹㊣㠚Ӕ ㌫⇽ᵜˈُࣙҾ㥹⭈㟖䲔㥹ࡲⲴ௧ᯭӗ⭏䳴ᙗн㛢ˈ⭘ਜ਼ᴹ NK603ǃ MON 88017 ㅹަᆳᣇ䲔㥹ࡲ䖜ॆһԦⲴ㠚Ӕ㌫⡦ᵜˈ⭏ӗⲴᵲӔ MON 87427 × NK603 ᡆ MON 87427 × MON 88017 ⭘Ҿ⭏ӗ䬰DŽ䘉ӋᵲӔ Ⲵṭษ䗷〻ѝˈߌ≁❦Պ᤹➗਼Ⲵ㥹⭈㟖ḷㆮ᧘㦀ᯩᔿ௧ᯭ䲔㥹ࡲDŽ 2008 ᒤ൘㖾ഭ⦹㊣Ἵ४ 6 њᐎǃ6 њ䈅傼⛩ሩਜ਼ᴹ MON 87427 䖜ॆһԦ Ⲵ⦹㊣ᵲӔ MON 87427 × NK603 ઼ MON 87427 × MON 88017 䘋㹼⭠䰤䈅傼ˈ ➗ḷㆮ᧘㦀֯⭘ᯩ⌅௧ᯭ㥹⭈㟖ˈỰ⍻࠶᷀Ҷ᭦㧧Ⲵ⦹㊣㊭㋂ѝ㥹⭈㟖৺ަԓ᤹ 䉒ӗ⢙≘⭢ส⼧䞨˄AMPA˅Ⲵ↻⮉≤ᒣ˄㿱ᢰᵟᣕ 14˅ˈഭ䱵伏૱⌅ިင ઈՊ˄Codex˅൘⦹㊣кᡰ䇮ᇊⲴ㥹⭈㟖ᴰབྷ↻⮉䲀䟿˄MRL˅ਚवᤜ㥹⭈㟖DŽ к䘠 MON 87427 ᵲӔ㥹⭈㟖ⲴỰ⍻㔃᷌㿱л㺘ˈ㺘ѝ MON 87427 ᵲӔ⦹ ㊣㥹⭈㟖↻⮉䟿ⲴỰ⍻㔃᷌о࡙⭘ㅜањᣇ㥹⭈㟖⦹㊣ GA21 ᔪ・Ⲵสᮠᦞ ・٬DŽ㘼สҾ GA21 䘋㹼Ⲵ࣐ᐕ㓴࠶↻⮉⹄ウᔪ สᵜа㠤ˈ䜭վҾ Codex MRL Ҷ䈕㊫⦹㊣⋩ǃ⦹㊣㊹Ⲵ↻⮉ᮠᦞˈࡇҾл㺘DŽ
XXXVI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᣇ㥹⭈㟖⦹㊣↻⮉䈅傼ᙫ㔃৺ Codex ᴰབྷ↻⮉䲀䟿≤ᒣ˄MRL˅ MON 87427 × MON 87427 × 㥹⭈㟖 GA21 ᵲӔ 1 NK603 ᵲӔ 2 MON 88017 ᵲӔ 2 㓴㓷㊫ර Codex MRL ѝսᮠ˄㤳ത˅ ѝսᮠ˄㤳ത˅ ѝսᮠ˄㤳ത˅ ppm ppm ppm ppm 0.11 0.04 0.06 ⦹㊣㊭㋂ 5 (<0.05 - 0.96) (0.01 - 0.13) (0.01 – 0.20) <0.04 ㋮⛬⦹㊣⋩ ᵚ䇮ᇊ ᵚ⍻ᇊ ᵚ⍻ᇊ (<0.02 – 0.32)3 0.12 ⦹㊣㊹ ᵚ䇮ᇊ ᵚ⍻ᇊ ᵚ⍻ᇊ (<0.05 – 1.1)3 1 ਜ਼ᴹ䖜ॆһԦ GA21 Ⲵㅜаԓᣇ㥹⭈㟖⦹㊣ᵲӔⲴ㥹⭈㟖↻⮉ᮠᦞˈ⭘Ҿᔪ・ 䈕㊫ᣇ㥹⭈㟖⦹㊣᭦㧧ӗ૱઼᭦㧧㊭㋂࣐ᐕ㓴࠶↻⮉สᮠᦞ˗ 2 ᮠᦞᶕ㠚 MON 87427 о NK603 ᡆ MON 88017 ↻⮉䈅傼˄ᢰᵟᣕ 14˅ ˅࣐ᐕ㓴࠶ѝⲴ↻⮉≤ᒣѝսᮠ઼㤳തᦞ㊭㋂↻⮉≤ᒣ઼ᓄ㓴࠶⎃㕙ഐᆀ˄CF 3 䇑㇇ᗇࡠDŽަѝ⦹㊣⋩ CF<0.33ˈ⦹㊣㊹ CF Ѫ 1.1DŽ к㺘ѝഭ䱵伏૱⌅ިငઈՊ˄Codex˅ᡰ䇮・Ⲵᴰབྷ↻⮉䲀䟿ᴰࡍᱟ൘ᑨ 㿴⦹㊣к䇮ᇊⲴDŽ൘ᣇ㥹⭈㟖⦹㊣кⲴ↻⮉䈅傼㔃᷌㺘᰾ˈ䲔㥹ࡲ㥹⭈㟖Ⲵ↻ ⮉≤ᒣվҾ Codex 䇮ᇊⲴ MRLDŽഐ↔⧠ᴹⲴ Codex MRL ᰒ䘲⭘Ҿᑨ㿴⦹㊣ˈ ҏ䘲⭘Ҿᣇ㥹⭈㟖䲔㥹ࡲ⦹㊣DŽྲ᷌⦹㊣࣐ᐕ㓴࠶Ự⍻ࡠⲴ↻⮉䟿≤ᒣ᰾ᱮ儈 ٬DŽն Codex ᡆ䇨Պ䪸ሩ࣐ᐕ㓴࠶䇮ᇊу䰘Ⲵ MRLˈ٬ Ҿ㊭㋂к䇮ᇊⲴ MRL ⭡Ҿ⦹㊣⋩ǃ⦹㊣㊹ㅹ㓴࠶ѝỰ⍻ࡠⲴ㥹⭈㟖Ⲵ↻⮉䟿䘌վҾ㊭㋂кⲴ MRL ٬DŽ ppm˅ˈഐ↔⋑ᴹу䰘䇮・⦹㊣㊹ǃ⦹㊣⋩Ⲵ MRL 5˄٬ ߌ㦟ᴰབྷ↻⮉䲀䟿˄MRL˅ᱟᤷ伏૱ᡆߌӗ૱䜘ᡆ㺘䶒⌅ᇊݱ䇨Ⲵߌ㦟 ᴰབྷ⎃ᓖˈԕ⇿ॳݻ伏૱ᡆߌӗ૱ѝߌ㦟↻⮉Ⲵ∛ݻᮠ㺘⽪˄mg/kg ᡆ ppm˅DŽ ߌ㦟ᴰབྷ↻⮉䲀䟿˄MRL˅ᱟ䙊䗷ሩߌ㦟䘋㹼ᒯ⌋Ⲵ∂⨶ᆖ䈅傼઼ሩҾᴍ䵢 ᓧӗ⭏ڕ䇱нՊሩӪ㊫؍䟿Ⲵ㟣伏仾䲙䇴ՠˈ⺞ᇊⲴਟ᧕ਇⲴߌ㦟↻⮉≤ᒣˈԕ н㢟ᖡ૽DŽߌ㦟ᴰབྷ↻⮉䲀䟿ᇎ䱵кᱟߌ㦟ਸ⨶֯⭘઼ߌӗ૱䍨᱃ᡰ䴰㾱䚥ӾⲴ ḷ߶DŽᴹӋഭᇦᦞ⌅ᖻ⌅㿴Ⲵ㾱≲ˈՊࡦᇊᵜഭߌӗ૱ᴰབྷ↻⮉䲀䟿ḷ߶ˈ㘼 Codex MRL Ѫഭ䱵伏૱⌅ިငઈՊ伏૱ḷ߶Ⲵа䜘࠶ˈ⭡ Codex ߌ㦟↻⮉င ઈՊᦞ FAO/WHO ߌ㦟↻⮉㚄ᑝՊ䇞˄JMPR˅Ⲵᔪ䇞ࡦᇊⲴDŽJMPR ᱟ⭡㚄 ਸഭ㋞ߌ㓴㓷઼ц⭼ছ⭏㓴㓷уᇦޡ਼㓴ᡀⲴ、ᆖ亮䰞㓴㓷ˈJMPR 䙊䗷ሩߌ㦟 ˅ᆖᮠᦞ઼↻⮉ᮠᦞ䘋㹼䇴ԧˈᦞ䇴ԧ㔃䇪ˈੁഭ䱵伏૱⌅ިငઈՊ˄Codex⨶∂ ᔪ䇞ᴰབྷ↻⮉䲀䟿ḷ߶˄MRL˅DŽ ᙫѻˈ䙊䗷↻⮉䈅傼ˈ࠶᷀Ự⍻Ҷ MON 87427 н਼⦹㊣ᵲӔᡰ᭦㧧㊭㋂ Ⲵ㥹⭈㟖↻⮉≤ᒣDŽ൘㊭㋂ѝỰ⍻ࡠⲴᴰ儈↻⮉䟿䘌վҾ Codex ൘ᑨ㿴⦹㊣к 䇮・Ⲵᴰབྷ↻⮉䲀䟿≤ᒣDŽสҾ GA21 ᔪ・Ⲵส↻⮉ᮠᦞ㺘᰾ˈሩҾ⦹㊣㊭ ㋂ˈ⦹㊣⋩ѝⲴ㥹⭈㟖↻⮉≤ᒣᶱབྷ䱽վˈ⦹㊣㊹↻⮉≤ᒣ∄㊭㋂〽ᴹ⎃㕙DŽ ᰐ䇪ᱟ⦹㊣㊹䘈ᱟ⦹㊣⋩ˈ㥹⭈㟖↻⮉䟿䜭䘌վҾ Codex ൘⦹㊣㊭㋂к䇮ᇊⲴ ٬DŽ MRL
XXXVII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߌъ䜘ޣҾMON 87427ᆹޘ䇴ԧ⭣䈧Ⲵഎ༽࠭ ˄2013ᒤ5ᴸ21ᰕ˅
XXXVIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ਮީӄߒѐ䜞གྷؗ࠳Ⲻ䈪᱄ޢᆕኧ䜳
⍻Ựޘ䰞仈 1˖ᵚᨀߌъ䜘ငᢈỰ⍻ᵪᶴࠪާⲴ䖜สഐ⦹㊣Ⲵ⧟ຳ઼伏⭘ᆹ ᣕDŽ 䈤᰾˖᤹➗ߌъ䜘、ᢰਁኅѝᗳޣҾᣇ䲔㥹ࡲ⦹㊣ MON 87427 Ⲵ⧟ຳᆹޘ઼ 伏⭘ᆹޘỰ⍻ԫ࣑л䗮䙊⸕ˈ⭡ߌߌъ䜘䖜สഐ⭏⢙伏⭘ᆹޘⴁⶓỰ傼⍻䈅ѝᗳ ˄ཙ⍕˅᳘ཙ⍕ᐲ⯮⯵亴䱢᧗ࡦѝᗳ઼ߌߌъ䜘䖜สഐἽ⢙⧟ຳᆹޘⴁⶓỰ傼⍻ 䈅ѝᗳ˄䮯᱕˅᳘ਹ᷇ⴱߌъ、ᆖ䲒࠶࡛ᆼᡀҶ MON 87427 ⦹㊣Ⲵ伏⭘ᆹޘ ઼⧟ຳᆹޘỰ⍻ԫ࣑ˈޣỰ⍻ᣕ࠶࡛㿱⭣䈧Җлᢰᵟᣕ 12 ઼ᢰᵟᣕ 13DŽ 伏⭘ᆹޘỰ⍻㔃᷌㺘᰾˖䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ MON 87427 ѝᣇ㩕 ޫഐᆀἽ䞨ਜ਼䟿Ѫ 20.3 mg/gˈ䶎䖜สഐᑨ㿴⦹㊣ EXP262 ѝἽ䞨ਜ਼䟿Ѫ 20.2 mg/gˈ є㘵⋑ᴹᱮ㪇ᐞᔲDŽ ሶ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ MON 87427 ઼䶎䖜สഐӢᵜ⦹㊣ EXP262 侢ᯉ˄∄ֻѪ 50% ǃ25% ǃ12.5%˅ல侢བྷ啐 90 ཙˈல侢ᵏ䰤ࣘ⢙⍫ޕ᧪࠶࡛ ࣘ㠚ྲˈ㻛∋ᴹݹ⌭ˈ啫ǃǃਓ㞄ᰐᔲᑨ࠶⋼⢙DŽ㔏䇑㔃᷌㺘᰾䜘࠶ᑨ㿴ሩ➗ ৺䖜สഐਇ䈅⢙㓴ࣘ⢙䘨伏䟿оᑨ㿴ส侢ᯉሩ➗㓴∄䖳ˈᴹ㔏䇑ᆖѹ ˄P<0.05˅ˈն൷ᰐࡲ䟿৽ᓄޣ㌫ˈᵚ㿱ަ⭏⢙ᆖѹDŽަ։㓴ࣘ⢙փ䟽ǃ䘋 伏䟿ǃᙫ໎䟽ǃᙫ䘋伏䟿઼ᙫ伏⢙࡙⭘⦷оᑨ㿴ส侢ᯉሩ➗㓴∄䖳ˈᐞᔲ൷ᰐ 㔏䇑ᆖѹ˄P>0.05˅DŽ ᇎ傼ѝᵏ઼ᇎ傼ᵛᵏ亩㹰⏢ᆖᤷḷ˄㓒㓶㜎䇑ᮠǃ㹰㓒㳻ⲭǃ㹰ሿᶯǃⲭ 㓶㜎䇑ᮠ৺࠶㊫˅ǃ㹰⭏ॆᤷḷ˄㹰щ≘䞨≘ส䖜〫䞦ǃཙ䰘ߜ≘䞨≘ส䖜〫 䞦ǃቯ㍐≞ǃ㛶പ䞷ǃ⭈⋩й䞟ǃ㹰㌆ǃң䞨㝡≒䞦ǃᙫ㳻ⲭǃⲭ㳻ⲭǃ㚼䞀ǃ ᙗ⼧䞨䞦˅൷൘ᵜỰ⍻অս↓ᑨ٬㤳തDŽࣘ⢙䀓ࢆᰦˈབྷփ㿲ሏᵚਁ⧠ᔲᑨDŽ⻡ 䳴ᙗࣘ⢙Ӣѝ㓴㛮/փ∄վҾሩ➗㓴ˈӢ儈㓴㛍/փ∄儈Ҿሩ➗㓴ˈ䖜վ㓴㛍/փ∄ 儈Ҿሩ➗㓴ˈ䖜վ㓴ᗳ/փ∄儈ҾӢվ㓴ˈ䖜ѝ㓴㛮к㞪/փ∄儈ҾӢѝ㓴ˈᐞᔲ ᴹ㔏䇑ᆖѹ˄P<0.05˅DŽ䳼ᙗࣘ⢙Ӣ儈㓴㜨㞪/փ∄ǃ㛮/փ∄儈Ҿሩ➗㓴ˈ䖜 ѝ㓴㛍/փ儈Ҿሩ➗㓴ˈ䖜儈㓴㜨㞪/փ∄ǃ㛮/փ∄儈Ҿሩ➗㓴ˈ䖜ѝ㓴㛍/փ儈Ҿ Ӣѝ㓴ˈᐞᔲᴹ㔏䇑ᆖѹ˄P<0.05˅DŽնк䘠ᐞᔲᰐࡲ䟿৽ᓄޣ㌫ˈᵚ㿱ަ⭏ ٬оሩ➗㓴∄䖳ˈᐞᔲ൷ᰐ㔏䇑ᆖ∄ᆖѹDŽަ։㓴ࣘ⢙㜿ಘ䟽䟿઼㜿/փ⢙ ѹ˄P<0.05˅DŽ⯵⨶㓴㓷ᆖỰḕᵚਁ⧠䈕䖜สഐṧ૱ᕅ䎧ᇎ傼ࣘ⢙ިර⯵⨶㓴 㓷ᆖ᭩ਈ઼⢩ᔲᙗᦏՔDŽ 㔬кˈ90 ཙབྷ啐ޘ伏૱ல侢䈅傼㺘᰾ˈ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ 侢ᯉ˄∄ֻѪ 50% ǃ25% ǃޕ᧪MON 87427 ઼䶎䖜สഐӢᵜ⦹㊣ EXP262 ࠶࡛ 12.5%˅ல侢བྷ啐 90 ཙˈᵚ㿱ަሩࣘ⢙ӗ⭏н㢟ᖡ૽DŽ ⧟ຳᆹޘỰ⍻㔃᷌㺘᰾˖ਇߌъ䜘Ⲵငᢈˈߌъ䜘䖜สഐἽ⢙⧟ຳᆹޘⴁⶓ Ự傼⍻䈅ѝᗳ˄䮯᱕˅᳘ਹ᷇ⴱߌъ、ᆖ䲒Ҿ 2014 ᒤᆼᡀҶሩ MON 87427 ᣇ䲔 㥹ࡲ⦹㊣Ⲵ⧟ຳᆹޘᙗ䇴ՠDŽ
XXXIX ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䲔㥹ࡲ㙀ਇᙗ䢤ᇊ㔃᷌㺘᰾ˈо䶎䖜สഐ⦹㊣ሩ➗૱∄ˈ௧ᯭ᧘㦀ࡲ䟿 ৺ 2 ؽ᧘㦀ࡲ䟿ߌ䗮䲔㥹ࡲˈሩ䖜สഐ⦹㊣ MON87427 ἽṚⲴ⭏䮯ਁ㛢ᰐ᰾ᱮ ᖡ૽ˈ㺘᰾䖜สഐᣇ䲔㥹ࡲ⦹㊣ MON87427 ሩߌ䗮䲔㥹ࡲާᴹ㢟ྭⲴ㙀ਇᙗDŽ ⭏ᆈㄎҹ㜭࣋Ự⍻㔃᷌㺘᰾ˈ൘㦂ൠ䈅傼ᶑԦлˈ䖜สഐ⦹㊣ MON87427 Ⲵ⭏ᆈㄎҹ㜭࣋ᱮ㪇ᕡҾᵲ㥹ˈфоަሩᓄⲴ䶎䖜สഐ⦹㊣ᰐᱮ㪇ᐞᔲ˗൘ṭษ ൠ䈅傼ᶑԦлˈ䖜สഐ⦹㊣ MON87427 ൘Ṛ儈ǃ㾶ⴆᓖǃӗ䟿ǃਾԓᆀਁ㣭 ⦷ㅹᯩ䶒ˈоሩᓄⲴ䶎䖜สഐ⦹㊣ᰐᱮ㪇ᐞᔲDŽ ሩ⭏⢙ཊṧᙗᖡ૽Ự⍻㔃᷌㺘᰾ˈо䶎䖜สഐ⦹㊣∄ˈ䖜สഐ⦹㊣ MON87427 ሩ⭠䰤ѫ㾱㢲㛒ࣘ⢙ᮠ䟿઼㗔㩭㔃ᶴǃѫ㾱匎㗵ⴞᇣ㲛ԕ৺ѫ㾱⯵ᇣ ᰐᱮ㪇ᖡ૽DŽ
㔬ਸ䇴ԧᣕDŽޘ䰞仈 2˖ᵚᨀ䖜สഐ⦹㊣Ⲵ⧟ຳ઼伏⭘ᆹ 䈤᰾˖ᣇ䲔㥹ࡲ䖜สഐ⦹㊣ MON 87427 Ⲵ⧟ຳᆹޘ઼伏⭘ᆹޘ㔬ਸ䇴ԧᣕ 䱴Ԧ䍴ᯉāѝ˄ㅜ 174 亥lj䖜 cp4 epspsޣ䜘࠶Āޝᐢ㺕ݵ㠣ᵜ⭣䈧Җ↓᮷ㅜ สഐ㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣ MON 87427 Ⲵ⧟ຳ઼伏⭘ᆹޘᙗ㔬ਸ䇴ԧᣕ NJ˅DŽ
XL ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ഭཆᆹޘ䇴ԧᢰᵟᣕⴞᖅ ˄ᢰᵟᣕ᮷৺㘫䈁Ԧ䈧㿱ᵜ⭣䈧Җѝлᇩ˅
⭣䈧Җѝ˄ᢰᵟᣕ1~8˅
㕆ਧ ᢰᵟᣕ仈ⴞ 亥⸱
ᣕ 1˖ MON 87427 ⦹㊣Ⲵ࠶ᆀ⢩ᖱ࠶᷀˄ᆏኡ䜭ޜਨ⹄ウᣕ˖ <1> MSL0021822˅
ᣕ 2˖ 2008 ᒤ㖾ഭ⭠䰤䈅傼 MON 87427 ⦹㊣㓴㓷ѝⲴ CP4 EPSPS <76> 㳻ⲭ㺘䗮≤ᒣ䇴ՠ˄ᆏኡ䜭ޜਨ⹄ウᣕ˖MSL0022370˅
ᣕ 3˖ MON 87427 ⦹㊣ཊцԓਦ⡷઼ᆀṧ૱ѝ CP4 EPSPS 㳻ⲭ < 110> Ⲵ Western ঠ䘩࠶᷀˄ᆏኡ䜭ޜਨ⹄ウᣕ˖MSL0022026˅
ᣕ 4a˖2008 ᒤ㖾ഭ⭠䰤䈅傼ѝ MON 87427 Ⲵ㺘ර઼⭏ᘱӂ䇴ԧ < 135 > ˄ᆏኡ䜭ޜਨ⹄ウᣕ˖MSL0022501˅
ᣕ 4b: 2008-2009 ᒤ㖾ഭབྷ⭠䖜สഐ⦹㊣ MON 87427 㠚⭏㤇▌࣋䇴 < 297 > ԧ˄ᆏኡ䜭ޜਨ⹄ウᣕ˖MSL000022540˅
ᣕ 5a˖MON 87427 ㊭㋂ᶕⓀ CP4 EPSPS 㳻ⲭⲴ⢩ᙗ䢤ᇊ৺Ἵ⢙ᶕ < 334 > Ⓚ઼བྷ㛐ᵶ㧼ᶕⓀⲴ CP4 EPSPS 㳻ⲭ⨶ॆᙗ䍘઼࣏㜭⢩ᙗ ∄䖳˄ᆏኡ䜭ޜਨ⹄ウᣕ˖MSL0023119˅
ᣕ 5b˖MON 87427 ⦹㊣㊭㋂ᨀਆⲴ CP4 EPSPS 㳻ⲭ઼བྷ㛐ᵶ㧼ӗ < 402 > ⭏Ⲵ CP4 EPSPS 㳻ⲭ⢩ᙗ࠶᷀˄ᆏኡ䜭ޜਨ⹄ウᣕ˖ SCR-2016-0287˅
ᣕ 6˖ ࡙⭘ᴤᯠⲴ AD_2016ǃTOX_2016ǃPRT_2016 ᮠᦞᓃሩ < 427 > ˖CP4 EPSPS Ⲵ⭏⢙ؑᆖ䇴ԧᴤᯠ˄ᆏኡ䜭ᢰᵟᣕ RAR-2016-0208˅
ᣕ 7˖ ࡙⭘ᴤᯠⲴ AD_2016ǃTOX_2016ǃPRT_2016ǃEST_2016ǃ < 441 > NT_2016 ઼ NR_2016 ᮠᦞᓃሩ MON 87427 Ⲵ⭏⢙ؑᆖ䇴 ԧᴤᯠ˄ᆏኡ䜭ޜਨ⹄ウᣕ˖RAR-2016-0445˅
ᣕ 8˖ CP4 EPSPS 㳻ⲭ൘⁑ᤏ㛳⏢ѝⲴ⎸ॆ⦷䇴ԧ˄ᆏኡ䜭ޜਨ⹄ < 479 > ウᣕ˖MSL0017566˅
XLI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⭣䈧Җл˄ᢰᵟᣕ9~18˅
㕆ਧ ᢰᵟᣕ仈ⴞ 亥⸱
ᣕ 9˖ ࣐✝༴⨶ሩ CP4 EPSPS ࣏㜭⍫ᙗⲴᖡ૽˄ᆏኡ䜭ޜਨ⹄ウᣕ < 530 > ˖MSL0023307˅
ᣕ 10˖CP4 EPSPS 㳻ⲭⲴሿ啐ᙕᙗਓᴽ∂ᙗ䈅傼˄ᆏኡ䜭ޜਨ⹄ウᣕ < 560 > ˖MSL0013077˅
ᣕ 11˖2008 ᒤ㖾ഭ⭠䰤䈅傼ѝ MON 87427 ⦹㊣〨⿶઼㊭㋂Ⲵᡀ࠶࠶ < 592 > ᷀˄ᆏኡ䜭ޜਨ⹄ウᣕ˖MSL0022340˅
ᣕ 12˖MON 87427 ⦹㊣伏⭘ᆹޘỰ⍻ᣕ˄ߌъ䜘䖜สഐ⭏⢙伏⭘ < 799 > ᆹޘⴁⶓỰ傼⍻䈅ѝᗳ˄ཙ⍕˅ˈ2014 ᒤ 10 ᴸ˅
ᣕ 13˖MON 87427 ⦹㊣⧟ຳᆹޘỰ⍻ᣕ˄ߌъ䜘䖜สഐἽ⢙⧟ຳ <823 > ᆹޘⴁⶓỰ傼⍻䈅ѝᗳ˄䮯᱕˅ˈ2014 ᒤ 10 ᴸ˅
ᣕ 14˖བྷ㛐ᵶ㧼㺘䗮㓟ॆⲴ CP4 EPSPS 㳻ⲭሩሿ啐Ⲵᙕᙗਓᴽ∂ᙗ <858> ⹄ウ˄ᆏኡ䜭ޜਨ⹄ウᣕ˖MSL0026454˅
ᣕ 15˖བྷ㛐ᵶ㧼ᶕⓀ CP4 EPSPS 㳻ⲭ㓿ਓ♼㛳 CD-1 ሿ啐Ⲵᙕᙗ∂ᙗ <918 > ⹄ウ-5000mg/kg փ䟽˄ᆏኡ䜭⹄ウᣕ˖MSL0027235˅
ᣕ 16a˖ᯭ⭘㥹⭈㟖ࡦࡲਾ MON 87427 ⦹㊣㋇࣐ᐕߌӗ૱ѝ㥹⭈㟖↻ < 987 > ⮉䟿࠶᷀˄ᆏኡ䜭ޜਨ⹄ウᣕ˖MSL0021925˅
ᣕ16b˖䎺亦௧ᯭ㥹⭈㟖䲔㥹ࡲਾˈMON 87427 ⦹㊣࣐ᐕ㓴࠶ѝ㥹⭈ < 998 > 㟖↻⮉䟿৺⎃㕙㌫ᮠ⍻ᇊⲴᙫ㔃ᣕ˄ᆏኡ䜭ޜਨ⹄ウᣕ˖ MSL0028092˅
ᣕ 17˖MON 87427 ⦹㊣㊭㋂⭘侢ᯉᡀ࠶ሩᯁ⛩৹ቮ几Ⲵᖡ૽䇴ՠ < 1008 > ˄ᆏኡ䜭ޜਨ⹄ウᣕ˖SE-2010-0626˅
ᣕ 18˖侢லਜ਼ MON 87427ǃሩ➗ᡆ৲➗⦹㊣㊭㋂侢ᯉⲴ㚹呑䓛փᙗ <1045 > 㜭઼ᇠᵰਾ亩৲ᮠ∄䖳˄ᆏኡ䜭ޜਨ⹄ウᣕ˖ MSL0022485˅
XLII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᇶ୶ъ䍴ᯉ˄CBI˅༠᰾؍
ᇶ୶ъ䍴ᯉ˄CBI˅DŽ؍ਨᔰਁᒦᤕᴹⲴޜᵜ⭣䈧Җवਜ਼Ҷ䇨ཊҾᆏኡ䜭 ᐘབྷⲴӪ࣋⢙࣋ˈ㓿䗷䮯ᰦ䰤Ⲵ⹄ウ㧧ޕਨᣅޜᇶ୶ъ䍴ᯉᱟ⭡ᆏኡ䜭؍䘉Ӌ ᗇⲴˈवᤜ⹄ウ䐟㓯Ⲵࡦᇊǃ㺘䗮䈳᧗ݳԦⲴ DNA ᒿࡇǃ䚇Րㆆ⮕ ઼ᆹޘ䇴ԧⲴᮠᦞㅹ䍴ᯉDŽᆏኡ䜭ޜਨⴞࡽਚੁц⭼ഭߌъ䖜สഐ⭏⢙ 䇴ԧ઼ᇑᢩDŽаᰖᆏኡ䜭ޘᇶ䍴ᯉˈ⭘Ҿ⌅㿴ᆹ؍㹼᭯ѫ㇑䜘䰘ᨀӔ䘉Ӌ୶ъ ޜਨⲴㄎҹሩ㧧ᗇ䘉Ӌ䍴ᯉˈㄎҹሩሶՊԕᖸቁӪ࣋⢙࣋ᣅޕ൘ᖸ⸝Ⲵᰦ 䰤ᔰਁࠪ㊫լ MON 87427 Ⲵᓄӗ૱ˈ䘉ሩᆏኡ䜭ޜਨᶕ䈤ᱟнޜᒣⲴDŽ ᆏኡ䜭ޜਨ൘ѝഭഭཆ䶒Ѥ⵰䇨ཊᢰᵟᇎ࣋ᖸᕪⲴㄎҹሩDŽഐ↔ˈᆏኡ䜭 㿴ᇊޣ䇴ԧ㇑⨶࣎⌅NJㅜйㄐㅜҼॱгᶑⲴᴹޘਨᦞljߌъ䖜สഐ⭏⢙ᆹޜ ᇶDŽ؍ᇶ䍴ᯉ䘋㹼؍ԕ৺ᵜ⭣䈧ҖⲴປ߉䈤᰾ˈ㾱≲ሩᵜ⭣䈧Җḷᴹ CBI Ⲵ୶ъ
XLIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 аǃ⭣䈧㺘 ঠഃۜġ
ྜᅧݮ࿙ಓ̝௦ᄃೠಃைζ
MON 87427 ࠩࣇဈᆴަٝၐॸԅ̝௦ᄃೠ ਼ံޔы ᅧ cp4 epsps ݮ࿙ઐ҂Єંົ ࣿၗ ౖ՛٤ഓંົ फι Վ Ƙᄑ ฑಓ ᅧݮ࿙ಓы MON 87427
ᄯำ ံ਼ ༰ Zea mays L. ᅧ फ༰ ۣ·ࢳc ံ೨೩೫c ଶᄵďଶຂ Đד ಓ LH198 × HiII ̝௦ԉމ 1 ԙส ံ਼ᄵ ы ݮ Agrobacterium tumefaciens ٢ಓ ы cp4 epsps ંԅ sp.CP4 ࿙ ݮ࿙ ޔᤐफ҂Єإಓ༰ٟટ ζӒ CP4 EPSPS ӱ͂ēರᅧݮ࿙ᄑઐЄ ಓ ٢ಓ ԅ ыં ݮ࿙ ಓ༰ٟટ ٢ಓ Escherichia coli ၻ PV-ZMAP1043 ؠ ํ ٢ಓ ํ ݮ࿙ޚγ ํ ٢ಓ ํ ݮ࿙فࣣ ͱ ݮ࿙Ѐᆴफ 2 ܤࢉࠚӽᅧئᅧݮ࿙ֺ֥
1 މᅧݮ࿙ಓ̝௦ԉ
1 މᅧݮ࿙ಓСଶ̝௦ԉ
Ωۜރᄯೋི ᅧݮ࿙ಓы ੦ڟெࣣ ೋིԅನcԙԤۤ Ωۜރᅧݮ࿙ಓы ೋ ࡂೄׂܬ ଛᅹำۜ ི ெࣣ ੦ڟؠ ଛᅹನcԙԤۤ ࣣ Ωۜރᅧݮ࿙ಓы ಓСೋི ଛᅹำۜ ெࣣ ੦ڟଛᅹನcԙԤۤ
1 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ڳથಃைರဈֳถďಘcᆑᄭcᄐຊೇĐ ௦ થಃைರဈભຫ 5 Ӧสы ౖ՛ၙՊပຫ٤ഓ ֥ఆӝζ ಃ Ӧสᄩ ࡂෳ୲ྜӦส ை Ӧสԙᄖ ဏΩ 100028 Ӧ ಃைఆༀ Ԩܥ ส ؠ Ҏყ Ԩᆐဏັ ࣣ ॓ຂఆༀ Ԩܥ Ҏყ Ԩᆐဏັ Ӧสы ౖ՛٤ഓ ֥ఆӝζ ॓ຂఆༀ Ԩܥ ཙ Ҏყ Ԩᆐဏັ ᅖྑюఆ ᄥ ༀ ι ѻಓભၥ
Ӧ ༰ॅ ᅥྜޏ೬ᄏ ۥԙႛҶಹۥನۥ ส ᄵݮ࿙ٝёٝᆴ ϵူюఆ ٝדؠ ༀ ભ ༰ॅ ᄏы Ӧส ၽ·ົંᄯԅ
ࣣ
2 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 4 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 5 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 6 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 7 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 8 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ҽǃ亩ⴞᇩ᪈㾱 5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦˄EPSPS˅ᒯ⌋ᆈ൘ҾἽ⢙઼ᗞ⭏⢙փˈ 䭞䞦ˈ㘼㧭㥹䞨䙄ᖴ䍏䍓൘Ἵ⢙઼ᗞ⭏⢙փᨀ㣣俉ޣᱟ㧭㥹䞨䙄ᖴѝⲴањ ⧟ˈ⭘Ҿ㣣俉᯿≘ส䞨˄㤟щ≘䞨ǃ䞚≘䞨ǃ㢢≘䞨˅઼䇨ཊ⅑㓗⢙˄ྲഋ≒ਦ 䞨ǃ⌋䞼ǃ㔤⭏㍐ Kǃ㣡䶂㍐ǃ㊫哴䞞ǃᵘ䍘㍐ㅹ˅Ⲵ⭏⢙ਸᡀ˄Herrmann and Weaver 1999; Maeda and Dudareva 2012˅DŽ䈕䙊䐟൘Ἵ⢙փ䶎ᑨ䟽㾱ˈἽ⢙փ㠣 ቁ 35%Ⲵᒢ⢙䍘ᶕⓀҾ㧭㥹䞨䙄ᖴⲴӗ⢙˄Franz et al. 1997˅DŽԕ㥹⭈㟖Ѫᴹ᭸ ᡀ࠶Ⲵ䲔㥹ࡲߌ䗮®ᱟа㊫䶎䘹ᤙᙗ䲔㥹ࡲˈ㥹⭈㟖ਟԕᣁࡦἽ⢙ⓀⲴ EPSPS 䞦⍫ᙗˈӾ㘼䱫ᯝἽ⢙⭏䮯ᡰᗵ䴰Ⲵ㣣俉᯿≘ส䞨Ⲵ⭏⢙ਸᡀˈሬ㠤Ἵ⢙↫ӑˈ ᱟ䶎ᑨᴹ᭸Ⲵ䲔㥹ࡲˈ⭘Ҿ䱢⋫аᒤ⭏ᡆཊᒤ⭏Ⲵঅᆀਦᵲ㥹઼䱄ਦ㊫ᵲ㥹DŽ儈 ㅹ⭏⢙ྲପңࣘ⢙փнᆈ൘㧭㥹䞨䙄ᖴˈਚ㜭Ӿ伏⢙ѝ㧧ਆ㣣俉᯿≘ส䞨˗⭡ Ҿ㠚䓛н㜭ਸᡀ EPSPS 䞦ˈପңࣘ⢙ሩ㥹⭈㟖нᝏDŽ ᆏኡ䜭ޜਨᔰਁⲴ MON 87427 ⦹㊣˄OECD ୟа䇶࡛ਧѪ MON-87427-7˅ ਟԕ䘹ᤙᙗൠ൘аӋ㓴㓷ѝ㺘䗮ߌᵶ㧼 CP4 㧼ṚⲴ 5-✟䞷щ䞞䞠-3-⼧䞨䖜〫䞦 ˄CP4 EPSPS˅DŽMON 87427 ࡙⭘Ҷ⢩ᇊⲴࣘᆀ-ਜ਼ᆀ㓴ਸ˄e35S-hsp70˅ˈ ণ໎ᕪර 35S ࣘᆀ઼⦹㊣✝◰㳻ⲭ Hsp70 㕆⸱ᒿࡇⲴㅜањਜ਼ᆀDŽ䈕ࣘ ᆀ-ਜ਼ᆀ㓴ਸਟԕ⢩ᔲᙗൠ൘㩕ޫ⭏䮯ಘᇈ˄ਦ⡷ǃ〨⿶઼ṩ˅઼䳼ᙗ⭏⇆ಘ ֯ˈᇈ˄ਟԕ䘋а↕ਁ㛢ᡀᆀᡆ㊭㋂઼㣡эⲴ㓴㓷˅ѝ㺘䗮 CP4 EPSPS 㳻ⲭ 䘉Ӌ㓴㓷઼ಘᇈਟԕᣇ㥹⭈㟖DŽն CP4 EPSPS 㳻ⲭ൘㣡㊹ሿᆒᆀ˄ਟԕ䘋а↕ 㛢ᡀ㣡㊹㋂˅઼∑㔂ቲ㓶㜎˄Ѫ㣡㊹㋂ਁ㛢ᨀ㩕ޫ˅ѝ㺘䗮䟿ᶱվˈࠐѾᰐਁ ⌅Ự⍻ࡠDŽഐ↔ˈMON 87427 Ⲵ䳴ᙗಘᇈнᣇ㥹⭈㟖DŽ൘ MON 87427 ⦹㊣⢩ᇊ ⭏䮯䱦⇥˄V8~V13 ⭏䮯䱦⇥˅௧ᯭ㥹⭈㟖䲔㥹ࡲˈӗ⭏Ⲵ㣡㊹нާᴹ⍫࣋ˈഐ ↔ˈ൘⦹㊣ᵲӔࡦ䗷〻ѝˈਟԕ⭘ਜ਼ᴹ MON 87427 䖜ॆփⲴ⦹㊣㠚Ӕ㌫ ᵜˈُࣙҾ㥹⭈㟖䲔㥹ࡲⲴ௧ᯭӗ⭏䳴ᙗн㛢ˈݽ৫Ӫᐕᣭ䳴Ⲵ↕僔ˈབྷབྷ㢲⇽ 㓖ҶࡦᡀᵜDŽ MON 87427 ᱟ䙊䗷ߌᵶ㧼ӻሬ䖜ॆˈሶ PV-ZMAP1043 䍘㋂ѝⲴ T-DNA ሬ ޕࡠ LH198 × HiII ਇփ૱㌫ѝ㧧ᗇⲴDŽ䈕 T-DNA वਜ਼ᴹањ cp4 epsps สഐ㺘䗮 ⴂˈवᤜ e35S ࣘᆀǃhsp70 Ⲵਜ਼ᆀǃCTP2 ਦ㔯փ䖜〫㛭ᒿࡇǃcp4 epsps 㕆 ⸱ᒿࡇ઼ nos 㓸→ᆀDŽSouthern ᵲӔ࠶᷀㺘᰾ˈᶕ㠚Ҿ䖜ॆ䍘㋂ PV-ZMAP1043 ѝⲴ T-DNA ᒿࡇӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ൘⦹㊣สഐ㓴Ⲵঅњสഐᓗк˗ᒦфˈ 䲔Ҷ T-DNA ཆˈPV-ZMAP1043 䍘㋂Ⲵަᆳ䈳᧗ݳԦ˄वᤜ僘ᷦᒿࡇ˅൷⋑ᴹᨂ ޕࡠ MON 87427 Ⲵสഐ㓴ᒿࡇѝDŽPCR ઼ DNA ᒿࡇ࠶᷀䇱ᇎҶ T-DNA ᨂޕⲴ 㘬Ⲵสഐ㓴ᒿࡇDŽחᒿࡇޕ㔃ᶴᱟᆼᮤⲴˈަ㔃ᶴо䖜ॆ䍘㋂а㠤˗ᒦ⺞ᇊҶᨂ ↔ཆˈ䘈䙊䗷 Southern ᵲӔ࠶᷀䇱ᇎҶ MON 87427 ѝⲴཆⓀᨂޕ൘ 5 њ㛢ц ؍Ⲵ DNA ᒿࡇ൘ཊцԓ䰤ޕᙗ⣦Ⲵ䚇Ր࠶ᮠᦞҏ䇱ᇎᨂޕᤱっᇊˈሬ؍ԓ䰤 ᤱっᇊDŽ 㳻ⲭ䍘⢩ᖱ⢩ᙗ⹄ウ㔃᷌㺘᰾ˈMON 87427 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭоᐢ 㓿൘ѝഭԕ৺ц⭼ަԆഭᇦ㧧ᗇᢩ߶Ⲵཊᣇ㥹⭈㟖⢙ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭᱟ਼ⲴDŽ䘉Ӌӗ૱ѝⲴ CP4 EPSPS 㳻ⲭⲴᆹޘᙗᐢ㓿㻛བྷ䟿Ⲵᆹޘᙗ䇴 ᓧᖡ૽Ⲵڕ⢙Ҿ MON 87427 ሩӪ઼ପңࣘޣԧ⹄ウᡰ䇱ᇎDŽᵜ⭣䈧ҖᡰᨀⲴ 9 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 伏⭘ᆹޘ䇴ԧ䍴ᯉवᤜ˖1˅CP4 EPSPS 㳻ⲭⲴᆹޘᓄ⭘শਢ˗2˅CP4 EPSPS 㳻ⲭⲴ⨶ॆ⢩ᙗ઼࣏㜭࠶᷀˗3˅MON 87427 ⦹㊣ѝ CP4 EPSPS 㳻ⲭ㺘䗮䟿࠶ ᷀˗4˅࡙⭘⭏⢙ؑᆖᯩ⌅ሩ CP4 EPSPS 㳻ⲭоᐢ⸕Ⲵ∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃ ᓧᴹн㢟ᖡ૽Ⲵާᴹ⭏⢙⍫ᙗ㳻ⲭ䘋㹼ڕ⢙㠤㳻ⲭᡆަᆳᐢ⸕ሩӪ㊫઼ࣘ լᙗ࠶᷀ˈᵚਁ⧠ CP4 EPSPS ок䘠∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃ㠤㳻ⲭᡆᱟާ ᴹн㢟⭏⢙⍫ᙗ㳻ⲭѻ䰤ᴹԫօ㔃ᶴլᙗ˗5˅փཆ⎸ॆ࠶᷀㺘᰾ˈCP4 EPSPS 㳻ⲭ൘⁑ᤏ㛳⏢˄SGF˅ᶑԦл㻛䗵䙏⎸ॆ˗6˅✝っᇊᙗ࠶᷀㺘᰾ˈCP4 EPSPS 㳻ⲭ䲿⵰ᓖкॷަ⍫ᙗ䗵䙏їཡ˗7˅ṩᦞᴰᯠᆼᡀⲴሿ啐ᙕᙗਓᴽᇎ傼ˈ CP4 EPSPS 㳻ⲭⲴᰐ᰾ᱮᦏᇣ⭘ࡲ䟿˄NOAEL˅㠣ቁѪ 5000 mg/kg bwtDŽṩ ᦞѝഭӪ㗔㟣伏㩕ޫᩴޕᮠᦞԕ৺ MON 87427 ㊭㋂Ⲵ CP4 EPSPS 㳻ⲭਜ਼䟿 ˄3.6 μg/g fwt ᡆ 4.2 μg/g dwt˅ˈ䇑㇇ࠪⲴ᳤䵢䲀˄MOE˅儈䗮 6.9 × 106DŽ ṩᦞ OECD ᤷইˈ࠶᷀Ҷ MON 87427 ⦹㊣Ⲵ㊭㋂઼〨⿶Ⲵ㓴ᡀᡀ࠶ˈ वᤜ㩕ޫᡀ࠶ǃᣇ㩕ޫᡀ࠶઼⅑㓗ԓ䉒ᡀ࠶ˈᒦоަᑨ㿴ሩ➗૱䘋㹼∄䖳DŽ⭠ 䰤䈅傼৺ᡀ࠶࠶᷀ѝ䘈वᤜаӋᐢ㓿୶ъॆᓄ⭘Ⲵ⦹㊣૱ˈ⭘ᶕᔪ・ᑨ㿴⦹㊣ ᡀ࠶Ⲵ 99%ᇩ䇨४䰤DŽᡀ࠶࠶᷀㔃᷌㺘᰾ˈMON 87427 Ⲵᡀ࠶о䶎䖜 สഐሩ➗Ⲵᡀ࠶ᇎ䍘ㅹ਼ˈᒦф൷൘ᑨ㿴⦹㊣Ⲵ 99%ᇩ䇨४䰤DŽ ਇߌъ䜘Ⲵငᢈˈ2014 ᒤˈߌъ䜘䖜สഐ⭏⢙伏⭘ᆹޘⴁⶓỰ傼⍻䈅ѝᗳ ˄ཙ⍕˅᳘ཙ⍕ᐲ⯮⯵亴䱢᧗ࡦѝᗳ࡙⭘ MON 87427 ⦹㊣㊭㋂䘋㹼Ҷ伏⭘ᆹޘ Ự⍻DŽ㔃᷌㺘᰾ˈ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ MON 87427 ѝᣇ㩕ޫഐᆀἽ䞨 ਜ਼䟿Ѫ 20.3 mg/gˈ䶎䖜สഐᑨ㿴⦹㊣ EXP262 ѝἽ䞨ਜ਼䟿Ѫ 20.2 mg/gˈє㘵⋑ ᴹᱮ㪇ᐞᔲDŽሶ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ MON 87427 ઼䶎䖜สഐӢᵜ⦹㊣ 侢ᯉ˄∄ֻѪ 50% ǃ25% ǃ12.5%˅ல侢བྷ啐 90 ཙˈᵚ㿱ަޕ᧪EXP262 ࠶࡛ ሩࣘ⢙ӗ⭏н㢟ᖡ૽DŽᡰᴹԕк䘉Ӌ࠶᷀㔃᷌൷䇱ᇎ˖MON 87427 ⦹㊣৺ަ㺘 ᓧ⋑ᴹн㢟ᖡ૽DŽڕ䗮Ⲵ CP4 EPSPS 㳻ⲭሩӪ઼ପңࣘ⢙Ⲵ ↔ཆˈ䘈ሩ MON 87427 Ⲵ㺘රᙗ⣦ǃߌ㢪ᙗ⣦ԕ৺о⧟ຳӂ䘋㹼Ҷ䇴ՠˈ ᒦоަᑨ㿴⦹㊣ሩ➗䘋㹼∄䖳DŽ਼ᰦ൘䈅傼ѝ䘈वᤜҶаӋᐢ㓿୶ъॆἽⲴ૱ ˈ⭘ԕᔪ・ᑨ㿴⦹㊣ᙗ⣦Ⲵ㠚❦ਈᔲ㤳തˈԕׯ൘ࠪ⧠㔏䇑ᆖᱮ㪇ᙗᐞᔲᰦᶕ 䇴ՠ䘉Ӌᱮ㪇ᙗᐞᔲⲴ⭏⢙ᆖѹDŽ䇴ՠⲴᙗ⣦वᤜ˖ᆀՁⵐ઼㨼ਁ⢩ᙗǃ㣡 ㊹ᖒᘱᆖ⢩ᖱǃἽṚ㺘රᙗ⣦઼о⧟ຳӂㅹDŽ㔃᷌㺘᰾ˈMON 87427 Ⲵᆀ Ձⵐ઼㨼ਁ⢩ᙗо䶎䖜สഐሩ➗⋑ᴹᐞ࡛ˈоᑨ㿴⦹㊣аṧˈMON 87427 ⋑ᴹ ⺜ᇎᆀˈ㘼⺜ᇎᆀᱟᵲ㥹ᡰާ༷Ⲵިර⢩ᖱDŽMON 87427 ઼䶎䖜สഐሩ➗ ൘㣡㊹㋂ⴤᖴᯩ䶒⋑ᴹ㔏䇑ᆖᱮ㪇ᙗᐞᔲ˄ =0.05˅ˈMON 87427 Ⲵ㣡㊹⍫࣋〽 儈Ҿሩ➗˄99.7% vs. 98.9%˅ˈն MON 87427 Ⲵ㣡㊹⍫࣋о䶎䖜สഐሩ➗Ⲵᐞ࡛ ሿҾ 1%ˈਟԕ䇔Ѫ䘉ሿҾ 1%Ⲵᐞ࡛нާᴹ⭏⢙ᆖѹDŽ 㺘රᙗ⣦ǃߌ㢪ᙗ⣦઼о⧟ຳӂⲴ⭠䰤䈅傼㔃᷌ҏ䘋а↕䇱ᇎˈо䶎䖜ส DŽ⭠䰤ੁٮഐሩ➗∄ˈMON 87427 нՊ໎࣐⢙ᵲ㥹ॆᡆ䖜ਈѪᴹᇣ⭏⢙Ⲵ 䈅傼䇴ՠⲴᙗ⣦䲔Ҷवᤜ 14 њ⭏䮯ਁ㛢ᙗ⣦ཆˈ䘈वᤜἽṚሩ䶎⭏⢙㛱䘛৽ᓄ ԕ৺ἽṚо⯵ᇣ઼ἽṚо㢲㛒ࣘ⢙Ⲵӂޣ㌫DŽMON 87427 ઼䶎䖜สഐሩ➗ѻ 䰤ᆈ൘Ⲵୟаާᴹ㔏䇑ᆖᱮ㪇ᐞᔲⲴᮠᦞሩ∄ᱟཊ䈅傼⛩㔬ਸᮠᦞ࠶᷀ѝⲴᒬ 㤇⭏⍫࣋DŽMON 87427 Ⲵᒬ㤇⍫࣋վҾሩ➗˄2.4 㓗ሩ 2.7 㓗ˈ㓗࡛ࡂ࠶൘ 1-9 ѻ䰤˅DŽ⭡Ҿ䘉ᐞ࡛ᮠ٬ᖸሿˈᒦфᰐ䇪ᱟ MON 87427 䘈ᱟᑨ㿴ሩ➗Ⲵᒬ㤇⍫ 10 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ࣋ᮠ٬൷༴൘ᑨ㿴⦹㊣㠚❦ਈᔲⲴ㤳തˈഐ↔ਟԕ䇔Ѫ䘉㔏䇑ᆖᱮ㪇ᐞᔲн ާᴹ⭏⢙ᆖѹDŽަԆⲴሩ∄ѝˈ൘ἽṚሩ䶎⭏⢙㛱䘛৽ᓄ˄ᙫޡ 172 㓴ᮠᦞሩ ∄˅ǃ⯵ᇣ䙐ᡀⲴᦏᇣ˄ޡ 210 㓴ᮠᦞሩ∄˅ԕ৺㢲㛒ࣘ⢙䙐ᡀডᇣ˄ޡ 167 㓴 ᮠᦞሩ∄˅Ⲵ䇴ՠᯩ䶒ˈMON 87427 ઼䶎䖜สഐሩ➗䰤⋑ᴹ㔏䇑ᆖᱮ㪇ᐞᔲDŽ ↔ཆˈ൘⦹㊣ཌ㴮઼⅗⍢⦹㊣㷏ডᇣⲴ䇴ՠᯩ䶒ˈMON 87427 ઼ሩ➗䰤ҏнᆈ ൘㔏䇑ᆖᱮ㪇ᐞᔲDŽ䘉Ӌ㔃᷌ҏ䘋а↕㺘᰾ˈMON 87427 нՊ໎࣐⢙ᵲ㥹ॆ DŽੁٮᡆ䖜ਈѪᴹᇣ⭏⢙Ⲵ ൘ᴹᇣ㢲㛒ࣘ⢙઼ᴹ⳺㢲㛒ࣘ⢙ⲴѠᓖ䇴ՠѝˈሩ 4 њ䈅傼⛩Ⲵᮠᦞᙫޡ䘋 㹼Ҷ 203 㓴ሩ∄˄98 㓴ᴹᇣ㢲㛒ࣘ⢙ᮠᦞሩ∄઼ 105 㓴ᴹ⳺㢲㛒ࣘ⢙ᮠᦞሩ∄˅ˈ ަѝ 191 㓴ᮠᦞሩ∄ MON 87427 ઼䶎䖜สഐሩ➗䰤⋑ᴹ㔏䇑ᆖᱮ㪇ᐞᔲDŽ൘ 12 㓴ᆈ൘㔏䇑ᆖᐞᔲⲴᮠᦞሩ∄ѝˈᴹ 6 㓴ሩ∄ѝ MON 87427 Ⲵᴹᇣᡆᴹ⳺㢲㛒 ࣘ⢙ѠᓖⲴᒣ൷٬൘୶ъॆᑨ㿴૱Ⲵᮠ٬㤳ത˗ᴹ 6 㓴ᮠᦞሩ∄ѝ MON 87427 Ⲵᒣ൷٬㲭❦䎵ࠪ୶ъॆᑨ㿴૱Ⲵᒣ൷٬㤳തˈնᱟ䘉Ӌᐞᔲ൘ н਼䈅傼⛩䰤㺘⧠ᒦна㠤ˈഐ↔нާᴹ⭏⢙ᆖѹDŽ㔬ਸཊњ䈅傼⛩ᮠᦞⴻᶕˈ ˈੁٮоᑨ㿴⦹㊣∄ˈMON 87427 нՊ໎࣐⢙ᵲ㥹ॆᡆ䖜ਈѪᴹᇣ⭏⢙Ⲵ нՊ᭩ਈަሩ䶎⭏⢙㛱䘛ǃ⯵ᇣᡆ㢲㛒ࣘ⢙Ⲵ৽ᓄ઼㙀ਇᙗˈнՊሩ⧟ຳӗ⭏ᱮ 㪇ᖡ૽DŽ ਇߌъ䜘Ⲵငᢈˈߌъ䜘䖜สഐἽ⢙⧟ຳᆹޘⴁⶓỰ傼⍻䈅ѝᗳ˄䮯᱕˅᳘ ਹ᷇ⴱߌъ、ᆖ䲒Ҿ 2014 ᒤᆼᡀҶሩ MON 87427 ᣇ䲔㥹ࡲ⦹㊣Ⲵ⧟ຳᆹޘᙗ䇴 ՠDŽ㔃᷌㺘᰾ˈ䖜สഐᣇ䲔㥹ࡲ⦹㊣ MON87427 ሩ㠣ቁ 2 ؽ᧘㦀ࡲ䟿௧ᯭⲴߌ 䗮䲔㥹ࡲާᴹ㢟ྭⲴ㙀ਇᙗDŽ൘㦂ൠ઼ṭษൠ䈅傼ᶑԦлˈ䖜สഐ⦹㊣ MON87427 Ⲵ⭏ᆈㄎҹ㜭࣋оަሩᓄⲴ䶎䖜สഐ⦹㊣൷ᰐᱮ㪇ᐞᔲDŽо䶎䖜สഐ ⦹㊣∄ˈ䖜สഐ⦹㊣ MON87427 ሩ⭠䰤ѫ㾱㢲㛒ࣘ⢙ᮠ䟿઼㗔㩭㔃ᶴǃѫ㾱 匎㗵ⴞᇣ㲛ԕ৺ѫ㾱⯵ᇣᰐᱮ㪇ᖡ૽DŽ 㔬кᡰ䘠ˈMON 87427 ᱟᯠⲴ⦹㊣ࡦ઼ᆀ⭏ӗ㌫㔏ѝ䶎ᑨ䟽㾱Ⲵањ 䇴ԧ䍴ᯉ㺘᰾ˈMON 87427 䖜ॆ⭘Ⲵ䚇Ր㺘䗮о䈳᧗ݳԦᱟޘ㢲ˈབྷ䟿Ⲵᆹ⧟ ᆹޘⲴˈᒦф䖜ॆᯩ⌅ҏнՊ᭩ਈਇփⲴᆹޘᙗ˗ཆⓀสഐѪঅњս⛩Ⲵঅᤧ䍍 ᓧڕ⢙ф䚇Ր㺘⧠っᇊ˗MON 87427 㺘䗮Ⲵ CP4 EPSPS 㳻ⲭሩӪ㊫઼ࣘˈޕᨂ ⋑ᴹн㢟ᖡ૽ˈҏнҾ㠤ᡆ∂ᙗ㳻ⲭ˗MON 87427 ൘ᡀ࠶коᑨ㿴ሩ➗૱ ⧟ሩ⭏ᘱˈੁٮ⦹㊣ᱟᇎ䍘ㅹ਼Ⲵ˗MON 87427 ҏнާᴹ䖜ਈѪᴹᇣ⭏⢙Ⲵ ຳ⋑ᴹн࡙ᖡ૽DŽMON 87427 ⴞࡽᐢ㓿൘࣐བྷ઼㖾ഭ㧧ᗇ୶ъॆἽᢩ߶ˈ ǃਠ⒮ǃᰕᵜǃ丙ഭǃକՖ∄ӊǃ㨢ᖻᇮǃޠᒦݸਾ൘໘㾯କǃ◣བྷ࡙ӊ/ᯠ㾯 䎺ইǃ⅗ⴏㅹഭᇦ઼ൠ४㧧ᗇ䘋ਓᢩ߶DŽ ᆏኡ䜭ޜਨᲲᰦ⋑ᴹ䇑ࡂ൘ѝഭ⭣䈧Ἵ MON 87427 ⦹㊣ˈᵜ⭣䈧ӵѪҶ 㧧ᗇਟԕ⭘࣐ᐕᯉⲴᣇ䲔㥹ࡲ⦹㊣ MON 87427 ৺ަ࣐ᐕӗ૱Ⲵᆹޘ䇱ҖDŽ 䢤Ҿ MON 87427 ᐢ㓿㧧ᗇ㖾ഭ઼࣐བྷⲴ୶ъॆἽ䇨ਟˈѪҶнᖡ૽⦹㊣ӗ ૱䘋ਓˈᡆᱟ⭡Ҿ䖜สഐ⦹㊣վ≤ᒣᵲᑖᶕⲴ䍨᱃ѝᯝ仾䲙ˈᆏኡ䜭䘌ьᴹ䲀 ޜਨ᤹➗ѝॾӪ≁ޡ઼ഭߌъ䜘Ⲵޣ⌅㿴㾱≲ˈ⢩↔ᨀࠪ⭣䈧ˈ䈧≲㧧ᗇᣇ㥹 ⭈㟖䲔㥹ࡲ䖜สഐ⦹㊣ MON 87427 䘋ਓ⭘࣐ᐕᯉⲴᆹޘ䇱ҖˈᵋҸԕᢩ߶DŽ
11 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 йǃᐕⴞⲴ઼ѹ ⦹㊣ࡦ઼ᆀ⭏ӗᱟ⦹㊣⭏ӗⲴањ䶎ᑨ䟽Ⲵ⧟㢲DŽ䙊ᑨᛵߥл⦹㊣ᵲӔ ࡦ⭠㾱᤹аᇊ∄ֻἽ⡦⇽ᵜˈᒦфሩ⇽ᵜ৫䳴DŽ৫䳴ᱟᖫᓅԕ৺৫䳴ᱟ ৺ᰦሩᆀ䍘䟿ᴹᖸབྷᖡ૽DŽ਼ᰦˈ৫䳴ҏ㾱㙇䍩䖳ཊӪᐕDŽӪԜҏᴮ䈅മ䟷 ⭘䳴ᙗн㛢㌫ᢰᵟᶕࡦˈቭ㇑ҏᴹᖃ㿴⁑Ⲵᓄ⭘ˈնࡠⴞࡽѪ→࡙⭘䳴ᙗн 㛢ᢰᵟࡦ㓸ᆈ൘⵰䘉ṧ઼䛓ṧⲴ䰞仈DŽഐ↔ˈራᯠⲴࡦ䙄ᖴ઼ᢰᵟаⴤ ӪԜⲴⴞḷDŽ MON 87427 ᱟ䙊䗷䖜สഐᢰᵟሬޕ cp4 epsps สഐ㧧ᗇⲴDŽcp4 epsps สഐਟ ԕ㕆⸱ CP4 EPSPS 㳻ⲭDŽն⭡Ҿ MON 87427 Ⲵสഐ㺘䗮ⴂѝ䟷⭘ањ⢩↺Ⲵ ࣘᆀ઼ਜ਼ᆀ㓴ਸ˄e35S-hsp70˅ᶕ䈳᧗ CP4 EPSPS Ⲵ㺘䗮ˈᡰԕ MON 87427 ѝⲴ CP4 EPSPS 㳻ⲭ㺘䗮ᴹ㓴㓷⢩ᔲᙗDŽCP4 EPSPS 㳻ⲭ൘ MON 87427 Ⲵ㩕ޫ 㓴㓷ಘᇈ઼䳼ᙗ⭏⇆㓴㓷ಘᇈѝਟԕ↓ᑨ㺘䗮ˈն൘䳴ᙗ⭏⇆㓴㓷ಘᇈѝн㺘䗮 ᡆ㺘䗮䟿ᖸվDŽ࡙⭘䘉а⢩ᙗˈ㔃ਸ௧ᯭ㥹⭈㟖䲔㥹ࡲˈਟԕ൘ࡦ䗷〻ѝӪᐕ ࡋ䙐䳴ᙗн㛢DŽ ⦹㊣Ⲵ䳴ょਁ㛢Ҿ㩕ޫ⭏䮯䱦⇥Ⲵ V9 ᵏˈᒦф CP4 EPSPS 㳻ⲭ൘ MON 87427 Ⲵ䳴ᙗಘᇈѝн㺘䗮ᡆ㺘䗮䟿ᖸվˈഐ↔൘ MON 87427 ⭏䮯ਁ㛢Ⲵ V8-V13 䱦⇥௧ᯭ㥹⭈㟖䲔㥹ࡲਟԕᵰ↫㣡㊹⇽㓶㜎DŽ࡙⭘ਜ਼ᴹ MON 87427 䖜ॆ փⲴ㠚Ӕ㌫⇽ᵜˈ⭘ਜ਼ᴹަᆳᣇ㥹⭈㟖䖜ॆփⲴ㠚Ӕ㌫⡦ᵜˈ൘⦹㊣Ⲵ䘲ᇌ ⭏䮯䱦⇥௧ᯭ㥹⭈㟖ˈਟԕᶴᡀӪᐕн㛢㌫ࡦ㌫㔏DŽ ࡙⭘ MON 87427 䘋㹼⦹㊣ࡦ઼ᆀ⭏ӗˈާᴹԕлѹ˖ 1˅໎࣐ᵲӔࡦⲴ⚥⍫ᙗ˖⇽ᵜ৫䳴Ⲵޣ䭞ᰦᵏᱟӾ䳴ょᣭࠪࡠᮓ㊹䘉 ⇥ᰦᵏˈᒣ൷Ѫ 3-4 ཙDŽᖃࡽӪᐕ৫䳴Ⲵᯩ⌅वᤜᵪỠᣭ䳴˄а㡜㾱 2 ⅑˅઼ᐕᣭ䳴˄а㡜 3 ⅑˅DŽн䇪ଚᯩ⌅ˈ䜭䴰㾱൘ 3-4 ཙⲴᰦ䰤䟼 䳶ѝ䏣ཏⲴӪ઼࣋䇮༷DŽ㘼䟷⭘ MON 87427 ࡦˈਚ䴰㾱൘ V8-V13˄བྷ 㓖 14 ཙ˅Ⲵᰦ䰤௧⍂є⅑㥹⭈㟖䲔㥹ࡲDŽഐ↔ˈ⦹㊣ࡦⲴ⚥⍫ᙗᴤ 儈DŽ 2˅߿ቁᵲӔࡦⲴᡀᵜ˖䲿⵰㓿⍾ਁኅˈ䎺ᶕ䎺ཊߌъӪਓ䖜ੁᐲˈ ᒦфࣣࣘ࣋ᡀᵜⵏ൘нᯝкॷˈ䟷⭘Ӫᐕ৫䳴Ⲵᯩ⌅ሶՊ䶒Ѥ⭘ᐕ⸝㕪 ઼ᵲӔᆀᡀᵜሩ䖳儈ⲴᡈDŽ㘼䟷⭘ MON 87427 ࡦ㌫㔏Պᴹ ࣙҾ䀓ߣ䘉Ӌ䰞仈DŽ ⴞࡽˈᆏኡ䜭ޜਨ⋑ᴹ䇑ࡂ൘ѝഭ୶ъॆἽ MON 87427DŽ䢤Ҿ MON 87427 ᐢ㓿㧧ᗇ㖾ഭ઼࣐བྷⲴ୶ъॆἽ䇨ਟˈѪҶнᖡ૽⦹㊣ӗ૱䘋ਓˈᡆᱟ⭡Ҿ 䖜สഐ⦹㊣վ≤ᒣᵲᑖᶕⲴ䍨᱃ѝᯝ仾䲙ˈᆏኡ䜭䘌ьᴹ䲀ޜਨ᤹➗ѝॾӪ≁ ޡ઼ഭߌъ䜘Ⲵޣ⌅㿴㾱≲ˈ⢩↔ᨀࠪ⭣䈧ˈ䈧≲㧧ᗇᣇ㥹⭈㟖䲔㥹ࡲ䖜สഐ ⦹㊣ MON 87427 䘋ਓ⭘࣐ᐕᯉⲴᆹޘ䇱ҖˈᵋҸԕᢩ߶DŽ
12 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ഋǃഭཆޣ⹄ウⲴ㛼Ჟ䍴ᯉ ޘ⨳䖜สഐ⢙৺䖜สഐ⦹㊣ᓄ⭘ᛵߥ Ӿ 1996 ᒤࡠ 2017 ᒤˈ䖜สഐ⢙୶ъॆᐢ䎠䗷Ҷ 21 ᒤDŽ൘ 2016 ᒤˈ26 њഭᇦἽҶ 1.851 ӯޜ产Ⲵ䖜สഐ⢙ˈ∄ 2015 ᒤⲴ 1.797 ӯޜ产໎䮯Ҷ 3ˁ˗ ަѝ 19 њѪਁኅѝഭᇦˈ7 њѪਁ䗮ഭᇦ˗ਁኅѝഭᇦⲴἽ䶒〟ঐޘ⨳䖜ส ഐ⢙Ἵ䶒〟˄1.851 ӯޜ产˅Ⲵ 54%ˈ㘼ਁ䗮ഭᇦⲴἽ䶒〟ঐ 46%DŽӾ 1996 ᒤⲴ 170 зޜ产ࡠ 2016 ᒤⲴ 1.85 ӯޜ产ˈޘ⨳䖜สഐ⢙ⲴἽ䶒〟໎࣐໎䮯 ߌ≁Ӿѝਇ⳺䎵䗷 1500 ӯ㖾ݳ⨳ޘˈҶ 110 ؽ˄ISAAAϦ2017˅ˈ20 ᒤԕᶕ ˄ISAAAϦ2017˅DŽ㖾ഭǃᐤ㾯ǃ䱯ṩᔧǃ࣐བྷ઼ঠᓖӽ❦ᱟᧂࡽӄⲴᴰѫ 㾱䖜สഐ⢙Ἵഭᇦˈਸ䇑Ἵ䶒〟ঐࡠҶ 91%DŽ䲔↔ѻཆˈ㖾 10 ഭⲴ Ἵ䶒〟ޡ䇑Ѫ 8000 зޜ产ˈӊཚൠ४ 8 ഭἽ䶒〟ޡ䇑Ѫ 1860 зޜ产ˈ⅗ⴏ 4 ޜഭ˄㾯⨝⢉ǃ㪑㨴⢉ǃᯟ⍋Հݻǃᦧݻ˅Ⲵ䖜สഐ⦹㊣Ἵ䶒〟䗮ࡠ 13.6 з 产ˈই䶎઼㣿ѩҏ໎࣐Ҷ䖜สഐ⢙ⲴἽ䶒〟˄ISAAAϦ2017˅DŽ ༽ਸᙗ⣦ӗ૱ᱟ䘁ᒤᶕ໎䮯ᴰᘛⲴа㊫ᙗ⣦ˈ㲭❦ 2016 ᒤ㙀䲔㥹ࡲ⢙Ⲵ Ἵ䶒〟ӽঐޘ⨳䖜สഐ⢙Ἵ䶒〟Ⲵ 47%˄8650 зޜ产˅ˈնਜ਼ᴹєᡆ ԕкᙗ⣦Ⲵ༽ਸᙗ⣦䖜สഐ⢙Ἵ䶒〟ᐢ䗮ࡠ 7540 зޜ产ˈঐᙫ䖜สഐ⢙ Ἵ䶒〟Ⲵ 41%ˈ∄ 2015 ᒤ໎࣐ 29%DŽ㠚 2014 ᒤᔰˈб㖾⍢Ⲵᐤ㾯ǃ䱯 ṩᔧǃᐤ൝઼Ѽ൝䱶㔝ᔰἽᣇ㲛㙀䲔㥹ࡲⲴ༽ਸᙗ⣦བྷ䉶DŽቔަᱟᐤ㾯ˈ ᣇ㲛㙀䲔㥹ࡲⲴ Intacta བྷ䉶ഐѪ㢲㓖ߌ㦟઼䘲⭘ݽ㙅ᢰᵟ㘼൘ߌ≁ѝབྷਇ⅒䗾DŽ 䖜สഐ⦹㊣ᱟӵ⅑Ҿབྷ䉶ⲴㅜҼབྷ䖜สഐ⢙ˈ2016 ᒤἽ䶒〟ঐޘ⨳⦹ ㊣ᙫἽ䶒〟Ⲵ 26%DŽ䖜สഐ⦹㊣ԕᣇ㲛ᣇ䲔㥹ࡲ༽ਸᙗ⣦ѪѫˈቔަᱟӾ 2010 ᒤᔰˈਜ਼ᴹ 8 њสഐǃ3 ᙗ⣦Ⲵ SmartStaxTM ༽ਸᙗ⣦⦹㊣䘋ޕᐲ൪ˈᑞࣙ ߌ≁ᴤᴹ᭸ൠ䱢⋫ൠк઼ൠлᇣ㲛ˈ਼ᰦҏᨀᴹ᭸Ⲵᵲ㥹⋫⨶⇥DŽ䲿⵰䎺ই Ⲵ࣐ޕˈ2016 ᒤޘ⨳ᴹ 17 њഭᇦᢩ߶୶ъॆἽ䖜สഐ⦹㊣˄ISAAAϦ2017˅DŽ 2016 ᒤᱟѹ䶎ࠑⲴаᒤˈ䘉аᒤˈ䈪䍍ቄ྆㧧ᗇ㘵Ԝㅜа⅑ਁ㺘Ҷа亩 ༠᰾ˈ᭟ᤱ⭏⢙ᢰᵟˈ䚓䍓ԕ੩∋≲⯥Ⲵုᘱ৽ሩ䘉亩ᢰᵟ઼哴䠁བྷ㊣Ⲵᢩ䇴㘵DŽ 䖜ԕ৺ਟᤱ㔝ߌъ 2030ٿ㚄ਸഭ㋞伏оߌъ㓴㓷ǃഭ䱵伏⢙о᭯ㆆ⹄ウᡰǃ20 ഭ 䇞〻ᤷሬлⲴަᆳ㊫լᵪᶴ൷㠤࣋Ҿ൘ 15 ᒤᡆ㘵ᴤ⸝Ⲵᰦ䰤䀓ߣ侕侯઼㩕ޫ 䰞仈DŽᴤ䟽㾱Ⲵᱟˈ㖾ഭഭᇦ、ᆖ䲒ǃഭᇦᐕ〻䲒઼ഭᇦ५ᆖ䲒ਜ䳶Ҷ 50 ཊս 、ᆖᇦǃ⹄ウӪઈǃߌъ઼ᐕъуᇦˈ㓴ᡀᣕ㕆߉ငઈՊˈ൘䮯䗮єᒤⲴ㕆߉ ⹄ウˈ֯⭘Ҷ㠚䖜สഐ⢙俆⅑ਁ᰾ԕᶕǃ㠣Ӻ 20ޣ䗷〻ѝ৲㘳Ҷ 900 ཊ亩 ᒤ䰤〟㍟ⲴᮠᦞDŽ2016 ᒤ 5 ᴸ 19 ᰕˈ䈕уᇦငઈՊਁᐳᣕljสഐᐕ〻⢙˖ 㓿傼оኅᵋNJ〠ˈ䙊䗷สഐᐕ〻ἽⲴߌ⢙ਟԕᆹޘൠ伏⭘ˈнՊ໎࣐ᛓⱼ⯷ǃ 㛕㜆ǃ㛳㛐⯮⯵ǃ㛮⯵ǃ㠚䰝⯷ᡆ䗷Ⲵࠐ⦷˄https://nas-sites.org/ge-crops/˅DŽ аઘਾˈ㤡ഭⲷᇦᆖՊҏࠪ⡸ᇓՐ〠ˈоՐ㔏ߌ⢙∄ˈ䖜สഐߌ⢙нՊ ᓧ䙐ᡀᴤབྷডᇣDŽ䈕ሿᆀᱟ⭡㤡ഭ 6 䍴␡Ἵ⢙ᆖᇦ㓴ڕ⢙ሩ⧟ຳᡆӪ㊫઼ࣘ ᡀⲴуᇦ㓴ᆼᡀDŽᣕᤷࠪˈ“⋑ᴹ䇱ᦞ㺘᰾伏⭘аߌ⢙ᱟড䲙Ⲵ——ӵӵ ᱟഐѪᆳᱟ䖜สഐ伏૱”DŽ“㠚 18 ᒤࡽ䖜สഐߌ⢙⭏ӗㅜа⅑བྷ㿴⁑୶ъॆԕ ᶕˈ⋑ᴹԫօ䇱ᦞ䇱᰾ˈаӋн㢟ਾ઼᷌⎸䍩ԫօа㓿ᢩ߶Ⲵ䖜สഐߌ⢙ѻ
13 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䰤ᆈ൘ޣ㚄DŽ”ᣕ䘈ᤷࠪˈ䖜สഐߌ⢙⋑ᴹሩ⧟ຳᶴᡀԫօ⢩↺Ⲵডᇣ ˄https://royalsociety.org/~/media/policy/projects/gm-plants/gm-plant-q-and-a.pdf˅DŽ ∄˄䖜สഐ⢙Ⲵᐲ൪ԧ٬Ѫ 158 ӯ㖾ݳ⨳ޘᦞ Cropnosis ᵪᶴՠ㇇ˈ2016 ᒤ ⨳ ޘˈᣔᐲ൪ 735 ӯ㖾ݳᐲ٬Ⲵ 22%؍⢙⨳ޘᒤ໎䮯Ҷ 3%˅ˈঐ 2016 ᒤ 2015 ᐢ᭦㧧Ⲵ“ᴰ㓸ӗ૱”˄䖜สഐ⨳ޘ୶ъᆀᐲ൪ 45 ӯ㖾ݳᐲ٬Ⲵ 35%DŽ亴ᵏ ᱟ䖜สഐᆀঅ⤜ԧ٬Ⲵ 10 ؽԕкDŽޕᆳ᭦㧧Ⲵӗ૱˅Ⲵߌ൪ࠪ൪᭦ަ઼⢙ Klumper ઼ Qaim˄2014˅࡙⭘൘ц⭼ൠ䘋㹼Ⲵߌ൪䈳ḕᡆ⭠䰤䈅傼ᗇࠪⲴ ᮠᦞሩ䗷৫ 20 ᒤޘ⨳ 147 亩ᐢ⸕Ⲵ䖜สഐ⢙⹄ウ䘋㹼Ҷѕ䉘Ⲵ㔬ਸ࠶᷀ˈ ᒦᣕҶ䖜สഐབྷ䉶ǃ⦹㊣઼ỹ㣡ሩ⢙ӗ䟿ǃߌ㦟֯⭘઼ߌ≁᭦⳺ᯩ䶒Ⲵᖡ૽DŽ 䈕㔬ਸ࠶᷀Ⲵ㔃䇪ᱟĀ䖜สഐ⢙൘ 1995 ᒤ㠣 2014 ᒤ䰤ӗ⭏Ҷཊ䟽䟽བྷ᭸⳺˖ ≁䟷⭘䖜สഐᢰᵟ֯ॆᆖߌ㦟Ⲵ֯⭘⦷䱽վҶ 37ˁˈ⢙ӗ䟿ᨀ儈Ҷ 22ˁˈߌ ࡙⏖໎࣐Ҷ 68ˁDŽᣇ㲛⢙Ⲵӗ䟿໎⳺઼ߌ㦟߿ቁⲴ〻ᓖབྷҾᣇ䲔㥹ࡲ⢙DŽ ਁኅѝഭᇦ∄ਁ䗮ഭᇦⲴӗ䟿઼࡙⏖໎⳺儈DŽā䈕㔃䇪ঠ䇱ҶԕᖰⲴޘ⨳ᒤᓖ⹄ ウᗇࠪⲴа㠤ᙗ㔃᷌˖Ӿ 1996 ᒤࡠ 2013 ᒤ䰤⢙ӗ䟿໎࣐Ⲵՠ㇇ԧ٬Ѫ 1333 ӯ㖾ݳˈަѝˈਁ䗮ഭᇦ㧧ᗇⲴ㍟䇑㓿⍾᭸⳺㓖Ѫ 652 ӯ㖾ݳˈਁኅѝഭᇦӗ⭏ Ⲵ㓿⍾᭸⳺㓖Ѫ 681 ӯ㖾ݳ˄Brookes and Barfoot, 2015˅DŽ Ӿ 1994 ᒤሿ㿴⁑Ἵࡠ 2016 ᒤˈޘ⨳ޡᴹ 40 њഭᇦ઼ൠ४˄39 њഭᇦ৺ ⅗ⴏ 28 ഭ˅ᢩ߶Ҷཊњ䖜สഐӗ૱䘋ਓ⭘Ҿ伏⭘ǃ侢⭘ᡆ⧟ຳ䟺᭮DŽ㠚 1994 ᒤԕᶕˈ䘉Ӌഭᇦޡ䇑ሩ 26 Ἵ⢙˄нवᤜᓧѳ俘ǃ⧛⪠ǃ⸞⢥⢋ㅹ㿲䍿Ἵ⢙˅ Ⲵ 392 њ䖜ॆһԦᆼᡀҶ 3768 亩⅑⌅㿴ᢩ߶DŽަѝˈ1777 亩ᢩ߶Ѫ伏⭘⭘䙄˄ⴤ ᧕֯⭘ᡆ⭘Ҿ࣐ᐕ˅ǃ1238 亩Ѫ侢⭘⭘䙄˄ⴤ᧕֯⭘ᡆ⭘Ҿ࣐ᐕ˅ǃ753 亩Ѫ⧟ຳ 䟺᭮ᡆἽᢩ߶˄ISAAAϦ2017˅DŽ⦹㊣ӽ❦ᱟ䖜ॆփ㧧ᢩᮠ䟿ᴰཊⲴ⢙˄29 њഭᇦ/ൠ४Ⲵ 218 њ䖜ॆփᢩ߶˅ˈަ⅑ᱟỹ㣡ǃ傜䫳㯟ǃ⋩㨌઼བྷ䉶DŽ
CP4 EPSPS 㳻ⲭ৺ᣇ㥹⭈㟖䲔㥹ࡲ䖜สഐ⢙Ⲵ⹄ウ䘋ኅ 5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦˄EPSPS˅ᒯ⌋ᆈ൘ҾἽ⢙઼ᗞ⭏⢙փˈ 䭞䞦ˈ㘼㧭㥹䞨䙄ᖴ䍏䍓൘Ἵ⢙઼ᗞ⭏⢙փᨀ㣣俉ޣᱟ㧭㥹䞨䙄ᖴѝⲴањ ⧟ˈ⭘Ҿ㣣俉᯿≘ส䞨˄㤟щ≘䞨ǃ䞚≘䞨ǃ㢢≘䞨˅઼䇨ཊ⅑㓗⢙˄ྲഋ≒ਦ 䞨ǃ⌋䞼ǃ㔤⭏㍐ Kǃ㣡䶂㍐ǃ㊫哴䞞ǃᵘ䍘㍐ㅹ˅Ⲵ⭏⢙ਸᡀ˄Herrmann and Weaver 1999; Maeda and Dudareva 2012˅DŽ䈕䙊䐟൘Ἵ⢙փ䶎ᑨ䟽㾱ˈἽ⢙փ㠣 ቁ 35%Ⲵᒢ⢙䍘ᶕⓀҾ㧭㥹䞨䙄ᖴⲴӗ⢙˄Franz et al. 1997˅DŽྲമ 1 ᡰ⽪ˈEPSPS ✟䞦⭘Ҿ㧭㥹䞨䙄ᖴѝⲴㅜ 6 ↕ˈ䍏䍓ۜॆሶ⼧䞨✟䞷ᔿщ䞞䞨˄PEP˅кⲴ 䞷ᔿщ䞞䞠ส䖜〫ࡠ㧭㥹䞨-3-⼧䞨˄S3P˅кˈ৽ᓄ⭏ᡀ 5-✟䞷ᔿщ䞞䞠㧭㥹䞨 2- -3-⼧䞨˄EPSP˅ˈ਼ᰦ䟺᭮ࠪᰐᵪ⼧䞨˄HPO4 ˅DŽ˄Alibhai and Stallings, 2001˅DŽ
14 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 PEP
Inhibitor:
Shikimate-3-phosphate Glyphosate 5-Enolpyruvyl-shikimate-3-phosphate S3P EPSP മ 1. EPSPS 䞦ۜॆ৽ᓄ⽪മ
˅EPSPS 䞦⭘Ҿ㧭㥹䞨䙄ᖴѝⲴㅜ 6 ↕ˈ䍏䍓ۜॆᓅ⢙㧭㥹䞨-3-⼧䞨˄S3P о✟䞷ᔿщ䞞䞨˄PEP˅㔃ਸˈ⭏ᡀ 5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨˄EPSP˅ˈ਼ᰦ 2- 䟺᭮ࠪᰐᵪ⼧䞨˄HPO4 ˅DŽ㥹⭈㟖˄Glyphosate˅ᱟ PEP Ⲵㄎҹᙗᣁࡦ⢙ˈ㥹⭈㟖 Ⲵᆈ൘ਟ䱫→ EPSPS 䞦ሩ PEP Ⲵ࡙⭘ˈ䱫ᯝἽ⢙փ㣣俉᯿≘ส䞨ⲴਸᡀˈӾ㘼ਁ ᥕ䲔㥹⭘DŽ
ԕ㥹⭈㟖Ѫᴹ᭸ᡀ࠶Ⲵ䲔㥹ࡲߌ䗮®ᱟа㊫䶎䘹ᤙᙗ䲔㥹ࡲˈ㥹⭈㟖ਟԕᣁ ࡦἽ⢙ⓀⲴ EPSPS 䞦⍫ᙗˈӾ㘼䱫ᯝἽ⢙⭏䮯ᡰᗵ䴰Ⲵ㣣俉᯿≘ส䞨Ⲵ⭏⢙ ਸᡀˈሬ㠤Ἵ⢙↫ӑˈᱟ䶎ᑨᴹ᭸Ⲵ䲔㥹ࡲˈ⭘Ҿ䱢⋫аᒤ⭏ᡆཊᒤ⭏Ⲵঅᆀਦ ᵲ㥹઼䱄ਦ㊫ᵲ㥹DŽ❦㘼㥹⭈㟖Ҿ䶎䘹ᤙᙗ䲔㥹ࡲˈ൘䱢⋫ᵲ㥹Ⲵ਼ᰦ䘈Պᵰ ↫ߌ⢙DŽഐ↔ˈᣇ㥹⭈㟖⢙Ⲵᓄ⭘ሩߌъ⭏ӗ⍫ࣘާᴹ䶎ᑨ䟽㾱ⲴѹDŽ俆 ݸˈᣇ㥹⭈㟖⢙Ⲵᓄ⭘ਟԕѪߌ≁ᑖᶕаᒯ䉡ᵲ㥹䱢䲔᧚ᯭ˗ㅜҼˈ㥹⭈㟖 ˗ᱟа⧟ຳ৻ྭරⲴ䲔㥹ࡲ˗ㅜйˈਟԕ֯㥹⭈㟖㊫䲔㥹ࡲ൘⢙⭏䮯ᵏᓄ⭘ ˈㅜഋˈᣇ䲔㥹ࡲ⢙઼䲔㥹ࡲ㓴ᡀⲴᵲ㥹䱢䲔㌫㔏ਟԕ᧘ᒯቁ㙅ᡆݽ㙅㙅ᯩᔿ 㲰઼㢲ⴱ㜭Ⓚ˗ㅜӄˈᨀվ㙇儈᭸Ⲵᵲ㥹䱢䲔ץᤱ൏༔≤࠶ǃ߿ቁ൏༔؍Ӿ㘼 ᢰᵟDŽ 、ᆖᇦ䮯ᵏԕᶕаⴤ㠤࣋ษ㛢ᣇ䲔㥹ࡲⲴ⢙૱ˈն䟷⭘Ր㔏Ⲵ㛢ᢰᵟ ˄ྲՐ㔏Ⲵ䈡ਈ઼䘹ᤙᢰᵟ˅䜭ᵚਆᗇᡀ࣏˄OECD, 1999˅DŽӾкц㓚 80 ᒤԓ ,ᔰˈӪԜᔰُࣙҾ䟽㓴 DNA ᢰᵟᶕᔰਁᣇ㥹⭈㟖⢙ˈᒦ㧧ᗇᡀ࣏˄Gasser 1989; Hinchee et al., 1993; Padgette et al., 1989; 1995; Mazur, 1989; Kishore and ֯׳Shah, 1988; Kishore et al., 1988˅DŽѫ㾱䟷⭘Ⲵ⨶ᡆᵪࡦᴹ 3 њᯩ䶒˖аᱟ Ⓚ EPSPS 䎵䟿㺘䗮˄Shah et al., 1986˅˗Ҽᱟሬޕањо㥹⭈㟖Ӣ઼ᙗվⲴཆ Ⓚ EPSPS สഐˈᶴᔪਖањԓ䉒䙊䚃˄Barry et al., 1992; Comai et al., 1983; Hinchee et al., 1993˅˗йᱟሬޕањਟ䱽䀓㥹⭈㟖Ⲵสഐ˄Barry et al., 1992˅DŽ ᣇ䲔㥹ࡲ⦹㊣ MON 87427 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭ↓ᱟ࡙⭘Ҷањо㥹⭈ ㊣Ⓚᙗ⦹گ㟖Ӣ઼ᙗվⲴ EPSPS 䞦ˈ൘㥹⭈㟖ᆈ൘ᰦˈCP4 EPSPS 㜭ཏԓ EPSPS 䞦Ⲵ࣏㜭ˈᤱ㔝ᨀἽ⢙փ⭏䮯ਁ㛢ᡰᗵ䴰Ⲵ㣣俉᯿≘ส䞨ࡽփDŽ CP4 EPSPS ᴰࡍᱟӾ൏༔ѝᑨ㿱Ⲵߌᵶ㧼˄Agrobacterium˅CP4 㧼Ṛѝ࠶ ᗇࡠⲴˈަоᣁࡦ⢙㥹⭈⼧Ⲵ㔃ਸᑨᮠ appKi[㥹⭈㟖]Ѫ 2.7 mMˈ㘼оཙ❦ᓅ⢙ PEP Ⲵ㔃ਸᑨᮠ appKm[PEP]Ѫ 12 μM˄Barry et al., 1992; Padgette et al., 1995a˅˗ 15 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 appK ᱟ䞦оḀॆਸ⢙Ⲵ㔃ਸᑨᮠˈᮠ٬䎺བྷˈ㺘᰾䈕ॆਸ⢙о䞦ਁ⭏㔃ਸᡰ 䴰㾱Ⲵ⎃ᓖ䎺བྷˈণ䞦о䈕ॆਸ⢙Ⲵ㔃ਸ㜭࣋䎺ᕡDŽCP4 EPSPS Ⲵ appK1[㥹⭈ ণ㥹⭈㟖ᆈ൘ᶑԦл CP4 EPSPS ӽˈ٬ᱟ᮷⥞ᣕ䚃ѝⲴᴰ儈٬∄[㟖]/appKm[PEP Ҿ઼ PEP 㔃ਸˈഐ↔䘲ਸҾᔰਁሩ㥹⭈㟖㙀ਇⲴ䖜สഐ⢙DŽੁٮᴤ❦ CP4 EPSPS ᱟ⭡ 455 њ≘ส䞨㓴ᡀⲴঅ䬮ཊ㛭ˈ⨶䇪࠶ᆀ䟿Ѫ 47.6 kDaDŽᣇ 㥹⭈㟖⢙ѝⲴ CP4 EPSPS 䙊ᑨ൘ N-ᵛㄟᑖᴹа⇥ਦ㔯փ䖜䘀㛭ˈ⭘Ҿᕅሬ CP4 EPSPS ᇊսࡠਦ㔯փкˈ䘉ᱟⓀᙗἽ⢙ EPSPS 䞦൘㓶㜎Ⲵս㖞DŽӾᒿ ࡇкⴻˈCP4 EPSPS оἽ⢙ᶕⓀⲴ EPSPS ާᴹ 25-30%Ⲵլᙗˈо㓶㧼ᶕⓀⲴ EPSPS ާᴹ 50-90%ⲴլᙗDŽ㲭❦൘㳻ⲭ䍘а㓗㔃ᶴк㊫ EPSPS Ⲵ≘ส䞨ᒿ ࡇᒦнॱ࠶լˈնⴞࡽᐢ⸕ⲴᡰᴹᶕⓀ EPSPS 䞦˄वᤜ CP4 EPSPS˅൘й㓗 ᤃᢁ㔃ᶴк儈ᓖа㠤˄Hammond, et al. 2013˅DŽӾᇊѹкᶕ䈤ˈ਼Ⓚ㳻ⲭᴹլ ⲴҼ㓗㔃ᶴ઼й㓗ᶴ䊑ˈӾ㘼ާᴹլⲴ࣏㜭˄Pearson, 2000˅DŽ CP4 EPSPS ԕঅփᖒᔿᢗ㹼ۜॆ࣏㜭ˈн䴰㾱䖵䞦ᡆ⢩↺䠁ᆀ䖵ࣙDŽ Ѫᵚоᓅ⢙㔃ਸⲴ CP4 EPSPSחCP4 EPSPS Ⲵй㓗Ღփ㔃ᶴྲമ 2 ᡰ⽪ˈA മᐖ Ѫоᓅ⢙ S3P 㔃ਸਾⲴ䰝ਸ⣦ᘱⲴ䞦DŽоަᆳח䞦ˈԕᔰ᭮⣦ᘱⲴᶴ䊑ᆈ൘˗ਣ EPSPS аṧˈCP4 EPSPS 䞦⭡є䜘࠶ॺ⨳⣦㔃ᶴฏ䙊䗷䬠䬮४䘎᧕ˈ⇿њॺ⨳ 㔃ᶴฏ൷⭡ཊњᒣ㹼Ⲵ-㷪㓴ᡀˈ䞦ۜॆ⍫ᙗս⛩սҾєњॺ⨳⣦㔃ᶴฏѻ⣦ ⢙䰤ˈ⍫ᙗս⛩䱴䘁ਜ਼ᴹ䖳ཊᑖ⭥㦧Ⲵ≘ส䞨↻สˈׯҾоᓅ⢙ӂ⭘DŽоᓅ S3P Ⲵ㔃ਸ䈡ሬ䞦Ⲵᶴ䊑ਁ⭏⛸ਈॆˈᵜĀᢃᔰā⣦ᘱⲴєњॺ⨳⣦㔃ᶴ ฏ䰝ਸˈׯҾ䞦઼ PEP Ⲵ㔃ਸ˄ྲ B മᡰ⽪˅DŽCP4 EPSPS ሩ㥹⭈㟖Ⲵ㙀ਇ↓ᱟ ⭡Ҿަ⢩↺Ⲵࠐօᆖᶴ䙐⢩⛩˄Funke, et al. 2006˅DŽA മѝ₉㢢⧟⣦㔃ᶴᱟ䶐䘁 ᆸ↻ส Glu-354؍ᴹⲴ儈ᓖޡC-ᵛㄟⲴㅜ 347-358 ս↻สˈवᤜ EPSPS 䞦ᇦ᯿ᡰ ઼ Arg-357ˈо PEP/㥹⭈㟖Ⲵ㔃ਸᴹޣDŽ⢩࡛䟽㾱Ⲵᱟ CP4 EPSPS ㅜ 100 սк 䬮䱫→Ҷ㥹⭈㟖о䞦Ⲵ㔃חⲴщ≘䞨↻ส˄㿱 B മѝ A100˅DŽщ≘䞨кⲴ⭢ส ਸ˄Funke, et al. 2006˅DŽӪѪሶㅜ 100 սкⲴщ≘䞨ケਈᡀ⭈≘䞨ਾˈケਈⲴ CP4 EPSPS 䞦ሩ㥹⭈㟖ਈᗇᝏDŽѻࡽҏᴹ⹄ウ㺘᰾ˈབྷ㛐ᵶ㧼 EPSPS ѝᓄ ս⛩ྲ᷌Ӿ⭈≘䞨ケਈᡀщ≘䞨˄Gly96Ala˅ˈ㜭ཏབྷབྷ䱽վަሩ㥹⭈㟖Ⲵᝏ ᙗˈն਼ᰦо PEP ⲴӢ઼ᙗҏབྷབྷл䱽˄Barry et al. 1992; Padgette et al. 1996˅DŽ ᤱ؍ CP4 EPSPS 䞦Ⲵ⤜⢩ᶴ䙐֯ᗇަሩ㥹⭈㟖нᝏˈն਼ᰦሩཙ❦ᓅ⢙ PEP ᖸ儈ⲴӢ઼ᙗDŽ
16 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ 2. CP4 EPSPS ⓗ୕㔤㔃ᯊ♧ពമ
ѪоᓅחѪᵚоᓅ⢙㔃ਸⲴ CP4 EPSPS 䞦ˈԕᔰ᭮⣦ᘱⲴᶴ䊑ᆈ൘˗ਣחമᐖ ⢙ S3P 㔃ਸਾˈ䞦Ⲵᶴ䊑ਁ⭏ҶਈॆˈᡀѪ䰝ਸ⣦ᘱDŽമѝ⧟⣦㔃ᶴᱟ䶐䘁 C-ᵛㄟ Ⲵㅜ 347-358 ս↻สˈ൘ᔰ᭮ᶴ䊑ᰦⲴ㠚⭡ᓖ䶎ᑨ儈ˈն൘䰝ਸ⣦ᘱлਈᗇᴹᒿॆˈ о䱴䘁 N-ᵛㄟⲴ≘ส䞨↻สӂ⭘ˈޡ਼㓴ᡀ⍫ᙗս⛩DŽ䘉њ⧟кਜ਼ᴹ EPSPS DŽ⹄ޣᆸ↻ส Glu-354 ઼ Arg-357ˈо PEP/㥹⭈㟖Ⲵ㔃ਸᴹ؍ᴹⲴ儈ᓖޡ䞦ᇦ᯿ᡰ .䘋ަо PEP Ⲵ㔃ਸ˄Funke, et al׳ウ㺘᰾ˈঅԧ䱣ᆀਟ㜭Պᖡ૽↔⧟Ⲵᶴ䊑ᒦ 2006˅DŽ EPSPS 䞦˄वᤜ CP4 EPSPS˅ާᴹ儈ᓖⲴᓅ⢙⢩ᔲᙗDŽⴞࡽᐢ⸕ሩ EPSPS ਈॆDŽ׳䞦ާᴹ⭏⢙ᆖѹⲴᓅ⢙ӵѪ S3P ઼ PEPDŽ㥹⭈㟖н㜭ਁ⭏ EPSPS 䞦 㧭㥹䞨оབྷ㛐ᵶ㧼ᶕⓀ EPSPS 䞦Ⲵ㔃ਸ㜭࣋䶎ᑨᕡˈо S3P ∄ˈ㧭㥹䞨䴰㾱
17 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˈ䶎ᑨ儈⎃ᓖ˄mM 㓗˅ᴹਟ㜭㻛 EPSPS 䞦ۜॆ˄Gruys, et al. 1992˅DŽ⹄ウ㺘᰾ Ἵ⢙փཙ❦ᆈ൘ S3P ㊫լ⢙ྲ⭢ส㧭㥹䞨ǃཾቬ䞨ǃҼ≒㧭㥹䞨䜭нᱟ EPSPS Ⲵᓅ⢙˄Franz, et al. 1997˅DŽ ٬ǃᓖ઼ᆀᕪᓖㅹഐ㍐ᖡ૽DŽ CP4 EPSPS 㳻ⲭⲴ䞦⍫ᙗ઼っᇊᙗਇ pH ٬䗷儈ᡆ䗷վ䜭Պ䱽վ䞦⍫ᙗˈpH ሿҾ 5.5 ᰦ ᴰ䘲䞦⍫ pH Ѫ 9-9.5ˈpH CP4 EPSPS 䞦⍫ࠐѾᆼޘїཡˈн䗷⭡ pH ᕅ䎧Ⲵ䞦⍫ਈॆᒦ䶎нਟ䘶˄Padgette et al. 1993˅DŽCP4 EPSPS ሩ✝нっᇊˈᓖ䎵䗷 75°C ᰦˈCP4 EPSPS Ⲵ࣏㜭⍫ ᙗণ䱽㠣Ự⍻䲀ԕлˈն࣐✝ᒦнᖡ૽㳻ⲭа㓗㔃ᶴⲴᆼᮤᙗ˄䈖㿱ᵜ⭣䈧Җѝ ㅜ 3.3 䜘࠶˅DŽ䘉ㅖਸ✝ࣘ࣋ᆖ⌅ࡉˈণᓖॷ儈Պ֯㳻ⲭ儈㓗㔃ᶴਈᙗˈᕅ䎧 䞦⍫ᙗїཡDŽCP4 EPSPS ሩ✝нっᇊⲴ⢩ᙗ㺘᰾ˈ൘ᐕъ࣐ᐕᶑԦлˈᴰ㓸Ⲵ䖜 สഐ⦹㊣ӗ૱ѝнཚਟ㜭ᆈ൘ާᴹ࣏㜭⍫ᙗⲴ CP4 EPSPS 㳻ⲭ˄Hammond and Jez 2011˅DŽоᡰᴹިරⲴ㳻ⲭ䞦㊫аṧˈCP4 EPSPS Ⲵۜॆ⍫ᙗ䘈ਇ৽ᓄⓦ⏢ѝ ᆀᕪᓖⲴᖡ૽DŽ↓ᑨⲴ䍞ᆈ㕃ߢ⏢˄1mMKCl˅ᶑԦлˈCP4 EPSPS Ⲵ⢩ᔲ ⍫ᙗᴰ儈㜭ཏ䗮ࡠ 5.5 U/mgDŽCP4 EPSPS Ⲵ⢩ᔲ⍫ᙗ䲿⵰ᆀᕪᓖ໎བྷ㘼ॷ儈ˈ 㠣 50 mM KCl ᰦˈ䞦⍫ᙗ㜭ཏ໎࣐ 2-2.6 ؽ˄Padgette et al. 1993˅DŽ →㠚 1996 ᒤㅜаֻ䖜สഐᣇ㥹⭈㟖བྷ䉶 40-3-2 ୶ъॆԕᶕˈ䗴ӺѪ cp4 epsps สഐᐢ㓿㻛䖜ޕཊ⢙ѝˈ൘ޘ⨳㤳ത㻛୶ъॆἽᒦབྷ䟿⎸䍩ˈ वᤜབྷ䉶ǃ⦹㊣ǃ⋩㨌ǃỹ㣡ǃ㤌㬯ǃ⭌㨌ㅹㅹˈ䘉Ӌ䖜สഐ⢙ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭާᴹᶱ儈ⲴլᙗDŽབྷ䟿Ⲵᆹޘ䇴ԧ䍴ᯉ㺘᰾ˈ䲔Ҷሩ㥹⭈㟖Ⲵ ᣇᙗѻཆˈᣇ㥹⭈㟖䖜สഐ⢙൘ӗ䟿ǃॆᆖᡀ࠶઼㩕ޫᡀ࠶ᯩ䶒оਇփ૱ ∄⋑ᴹ᭩ਈ˄Delannay et al., 1995; Hammond et al., 1995; Harrison et al., 1996; Padgette et al., 1995; Nida et al., 1996˅DŽ୶ъॆ䘁 20 ᒤᶕˈ䖜 cp4 epsps สഐⲴ ᣇ㥹⭈㟖㻛⢙Ӫ㊫઼ࣘ⢙ⴤ᧕ᡆ䰤᧕བྷ䟿⎸䍩ˈާᴹ䮯ᵏᆹޘᓄ⭘Ⲵ䇠ᖅDŽ ཆⓀสഐ㺘䗮䈳᧗৺ MON 87427 Ⲵᔰਁᓄ⭘ ቭ㇑䙊ᑨ䇔Ѫ CaMV35S ࣘᆀҾ㓴ᡀරࣘᆀˈնᴹӋ⹄ウ䇔Ѫ CaMV35S ࣘᆀ൘н਼㓶㜎ᡆ㓴㓷ѝᡰ䈳᧗ⲴⴞⲴสഐ㺘䗮䟿ᱟнаṧⲴ ˄Benfey and Chua, 1989; Terada and Shimamoto, 1990; Williamson, et al., 1989; Yang and Christou, 1990˅ˈণާᴹ㓴㓷⢩ᔲᙗ䈳᧗⭘DŽ䟷⭘ CaMV35S ࣘᆀ䈳 ᧗Ⲵᣕสഐ൘аӋἽ⢙Ⲵ㣡㊹ѝⲴ㺘䗮䟿䖳վ˄Sunilkumar, et al., 2002; Wilkinson, et al., 1997˅DŽ MON 87427 ѝ cp4 epsps สഐ⭡໎ᕪරⲴ 35S˄e35S˅ࣘᆀ䈳᧗DŽ㣡ὠ㨌 㣡ਦ⯵∂ 35S สഐⲴࣘᆀ˄CaMV35S˅वᤜ㔃ᶴฏ A˄Domain A˅઼㔃ᶴฏ B ˄Domain B˅є䜘࠶˄മ 3˅ˈަѝ㔃ᶴฏ B ਟԕ໎ᕪⴞḷสഐⲴ㺘䗮DŽe35S ࣘᆀᱟ൘ CaMV35S ࣘᆀⲴสк࣐Ҷањ㔃ᶴฏ B˄Benfey, et al., 1989˅DŽ ഐ↔ˈe35S ࣘᆀо CaMV35S ⭘ᯩᔿ਼ˈਚᱟ࣏㜭䘋а↕໎ᕪ˄McPherson and Kay, 1994˅DŽ MON 87427 䖜ॆ㺘䗮䖭փⲴ T-DNA ४ฏवਜ਼Ҷањ cp4 epsps สഐ㺘䗮 ⴂˈ䈕สഐ㺘䗮ⴂᶴᔪᴹ e35S ࣘᆀǃ⦹㊣ hsp70 สഐⲴㅜ 1 њਜ਼ᆀǃcp4 epsps สഐԕ৺ nos 㓸→ᆀDŽޣҾਜ਼ᆀⲴ⭘ᵪࡦⴞࡽ䘈нॱ࠶ᾊˈնਟԕ㛟ᇊⲴ
18 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᱟਜ਼ᆀՊ䙊䗷࢚᧕ᵪࡦ৲оἽ⢙Ⲵสഐ㺘䗮䈳᧗˄䉒ṁㄐㅹˈ2011˅ˈᒦф亪 ᔿ⭘ݳԦ˄cis acting element˅Պ᧗ࡦ㣡㊹⢩ᔲᙗ㺘䗮˄Eyal, et al., 1995˅DŽ MON 87427 ѝⲴ⢩ᇊࣘᆀ઼ਜ਼ᆀ㓴ਸ˄e35S-hsp70˅ਟԕᖸྭൠ䈳᧗ cp4 epsps สഐ൘н਼㓴㓷ѝⲴ㺘䗮ˈণ൘⦹㊣ṩǃ㤾ǃਦǃ䳼ᙗಘᇈ˄㣡эǃ ᷌ょ઼㊭㋂˅ѝ㺘䗮ˈ൘䳴ᙗಘᇈ˄㣡㊹ሿᆒᆀ઼∑㔂ቲ˅н㺘䗮ᡆ㺘䗮䟿ᖸվDŽ
മ3. CaMV 35S઼e35Sࣘᆀࡍ㓗㔃ᶴ⽪മ
19 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ӄǃ䖜สഐἽ⢙Ⲵᆹޘᙗ䇴ԧ 1. ։ὃ⢟Ⲻᆿޞᙝ䇺ԭ 1.1 ։ὃ⢟Ⲻ㜂Ქ䍺ᯏ ૂެᆹ〦؍ᆜθ 1.1.1 ਇփἽ⢙Ѫ⦹㊣ˈᆖѪ Zea mays L.ˈ؇Ѫ⦹㊣ˈҏ〠⦹㴰哽ǃ㤎㊣ǃ ἂᆀㅹDŽ 1.1.2 ࠼㊱ᆜ൦փ ṭษ⦹㊣൘࠶㊫кҾঅᆀਦἽ⢙㓢ǃᵜ、˄Gramineae˅ǃ⦹㴰哽᯿ ˄Maydeae˅ǃ⦹㴰哽˄Zea L.˅ǃ⦹㊣˄Zea mays L˅ǃṭษ⦹㊣ӊ˄mays˅DŽ ൘ᰙᵏⲴ࠶㊫㌫㔏ѝˈ⦹㴰哽ӵᴹṭษ⦹㊣˄Zea mays L.˅ањDŽWilkes ˄1967˅ሶ⦹㴰哽˄བྷ࠽㥹ĀTeosinteā˅ᖂޕ⦹㴰哽ˈᡀѪަѝⲴањӊ ˈवᤜєњ˖аᒤ⭏໘㾯କ⦹㊣˄Zea mexicana˅઼ཊᒤ⭏⦹㊣˄Zea perennis˅DŽ ṩᦞᯠⲴ⹄ウ㔃᷌ˈDoebley 亶ሬⲴ⹄ウሿ㓴ሩ⦹㴰哽࠶㊫অսⲴൠս 䘋㹼䟽ᯠࡂ࠶ˈࡦᇊҶᯠⲴ࠶㊫㌫㔏˄Doebley and Iltis, 1980˅˖
Family - Gramineae˄ᵜ、˅ Subfamily - Panicoideae˄哽ӊ、˅ Tribe - Maydeae˄⦹㴰哽᯿˅ Genus - Zea˄⦹㴰哽˅ Species - Zea mays L.˄⦹㊣˅ subsp. mays˄ṭษ⦹㊣ӊ˅
⦹㊣৺ަ䘁㕈⢙઼࠶㊫ᆖ࠶㊫ˈ䈧䈖㿱㺘 3DŽ 1.1.3 䈋僂⭞։ὃ⢟〃δᡌ㌱ε〦 MON 87427 Ⲵ䖜ॆਇփᱟ LH198 × HiII ᵲӔDŽLH198 ާᴹ㢟ྭⲴߌ㢪ᙗ ḃ∄䖳ᝏˈᒦфᇩ᱃⭏DŽץ㘼 HiII ሩߌᵶ㧼˗⣦ LH198 ᱟ⭡սҾ⡡㦧ॾᐎⲴ Holden’s ޜਨҾ 1992 ᒤษ㛢Ⲵ㠚Ӕ㌫ˈ ᶕ㠚ᵲӔ㓴ਸ˄LH132 × B84˅× LH132DŽLH132 ҏᱟ Holden’s ޜਨⲴษ㛢 Ⲵ㠚Ӕ㌫ˈ㘼 B84 ࡉᱟ⡡㦧ॾᐎ・བྷᆖษ㛢Ⲵ㠚Ӕ㌫DŽ HiII 㠚Ӕ㌫ᱟ䶎ᑨྭⲴ䖜ॆਇփˈᒦфޜՇਟԕᖸᇩ᱃ӾĀ⦹㊣䚇Ր䍴Ⓚ ѝᗳā㧧ᗇ˄Maize GDB, 2010˅DŽHiII ᶕ㠚ᵲӔ㓴ਸѪ B73 × A188 ˄Armstrong et al., 1991˅DŽ 1.1.4 ᱥ䠄⭕〃䘎ᱥṳฯ〃 MON 87427 Ⲵਇփ LH198 × HiII ৺ަᶕⓀ㠚Ӕ㌫൷ѪṭษDŽ
20 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘 3. ṭษ⦹㊣࠶㊫৺ަ䘁㕈⢙ 、 – ᵜ、 Gramineae ӊ、 – 哽ӊ、 Panicoideae ᯿ – ⦹㴰哽᯿Maydae 㾯ॺ⨳˖ I. - ⦹㴰哽 Zea A. ӊ – 㑱㤲ӊ Luxuriantes 1. 㑱㤲⦹㊣ Zea luxurians˄2n = 20˅ 2. ཊᒤ⭏㊫⦹㊣ Zea perennis˄2n = 40˅ ˅Ҽؽփཊᒤ⭏㊫⦹㊣ Zea diploperennis˄2n = 20 .3 B. ӊ - ⦹㴰哽ӊ Zea 1. ṭษ⦹㊣ Zea mays˄2n = 20˅ 1. ṭษ⦹㊣ӊ Z. mays mays˄2n = 20˅ 2. ሿ仆⦹㊣ӊ Z. mays parviglumis˄2n = 20˅ 3. ငင⢩ইᠸ⦹㊣ӊ Z. mays huehuetenangensis˄2n = 20˅ 4. ໘㾯କ⦹㊣ӊZ. mays mexicana˄Schrad.˅˄2n = 20˅ II. – ᪙ᬖ Tripsacum A. ӊ – Tripsacum B. ӊ – Fasciculata 1. T. andersomii˄2n = 64˅ 1. T. jalapense˄2n=72˅ 2. T. australe˄2n = 36˅ 2. T. lanceolatum˄2n =72˅ ਈ 3. T. fasciculatum˄2n = 36˅ a˅T. australe var. australe 4. T. maizar˄2n = 36, 72˅ b˅T. australe var. hirstum 5. T. pilosum˄2n = 72˅ 3. T. bravum˄2n = 36, 72˅ਈ 4. T. cundinamarce˄2n = 36˅ a˅T. pilosum var. guatemalense 5. T. dactyloides˄2n = 72˅ b˅T. pilosum var. pilosum ਈ a˅T. dactyloides var. hispidum b˅T. dactyloides var. dactyloides c˅T. dactyloides var. meridonale d˅T. dactyloides var. mexicanum 6. T. floridanum˄2n = 36˅ 7. T. intermedium˄2n = 72 8. T. manisuroides˄2n = 72˅ 9. T. latifolium ˄2n = 36˅ 10. T. percuvianum˄2n = 72, 90, 108˅ 11. T. zopilotense˄2n = 36, 72˅ ӊ⍢˖ I. – ⍱㣿᷌ Chionachne˄2n = 20˅ ⺜Ⳟ᷌ Schlerachne˄2n = 20˅ 㮿㤑 Coix ˄2n = 10, 20˅ й㻲᷌ Trilobachne˄2n = 20˅ ཊ㼄哽 Polytoca˄2n = 20˅ ᯿ – 儈㋡᯿ Andropogoneae I. - Manisuris
21 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 1.1.5 ӝ൦ᕋ䘑ᰬ䰪 བྷ䟿⹄ウ㺘᰾ˈ⦹㊣ᖸਟ㜭Ҿ 7,000 ࡠ 10,000ᒤࡽ൘໘㾯କই䜘ᔰ傟ॆDŽ 㲭❦⦹㊣ޜ䇔Ⲵ䎧Ⓚⴞࡽ䘈⋑ᴹࡠˈնᱟ໘㾯କ⦹㴰哽ᖸਟ㜭൘⦹㊣Ⲵ䚇Ր 㛼Ჟѝ䎧Ҷ䟽㾱⭘DŽ କՖᐳਁ⧠ᯠབྷ䱶ᰦˈӾᲪ࡙ࡠ࣐བྷই䜘Ⲵᖃൠ᮷᰾⽮Պ↓൘Ἵ⦹㊣DŽ କՖᐳҾ 1492 ᒤᤷࠪ൘ਔᐤই䜘⋯⎧ൠ४ᴹ⦹㊣Ἵˈᒦ൘䘄എ㾯⨝⢉ᰦᢺ⦹ ㊣ᕅޕҶ⅗⍢˄Goodman, 1988˅DŽ⦹㊣ᕅޕ⅗⍢ԕਾнѵˈ⦹㊣Ր䙽Ҷц⭼к ਟԕἽ⦹㊣Ⲵᡰᴹൠ४DŽ 䈤DŽٷሩҾ⦹㊣Ⲵ䎧Ⓚˈⴞࡽᴹഋњѫ㾱Ⲵ
1˅ ᶕ㠚Ҿ໘㾯କ⦹㴰哽˄৸〠㊫⦹㊣ᡆབྷ࠽㥹˅˖࡙⭘໘㾯କ⦹㴰哽䘹㛢 ӗ⭏Ⲵ⦹㊣˗
䈤˖a˅⦹㊣ᶕ㠚Ҿ᷌㦊⦹㊣˗b˅࡙⭘⦹㊣઼й㥹ᵲӔ㹽⭏Ⲵٷй䟽 ˅2 ໘㾯କ⦹㴰哽˗c˅䙊䗷⦹㊣਼໘㾯କ⦹㴰哽ᡆ㘵й㥹䰤ᵲӔˈᡆ 㘵਼Ҽ㘵਼ᰦ䰤ᵲӔˈ䘋ॆࠪᶕⲴ⧠ԓ⦹㊣૱˄Mangelsdorf, 1974˅˗
䈤˖⦹㊣ǃ໘㾯କ⦹㴰哽઼й㥹⤜・ൠᶕⓀҾ਼аᑨٷᲞ䙊䎧Ⓚ ˅3 ˗㿱նᵚ⸕Ⲵ⾆ݸ
䈤˖໘㾯କ⦹㴰哽⭡Ҿਾᶕ䚇Րᴹᙗケਈˈሬ㠤䳼ٷ⚮䳮ᙗᴹᙗケਈ ˅4 ょⲴਁ㛢ˈӾ㘼ӗ⭏Ҷ⧠ԓ⦹㊣DŽ
↔ཆ䘈ᴹаӋ᧘ᯝˈྲ Andropogoneae ᯿䟼Ⲵ Coix ઼ Manisuris Ⲵ䚇Ր㓴 ᙗǃ਼ޡ䈤ᐢ㓿䙊䗷⹄ウᵲӔⲴสഐ㓴ٷᡀҏሩ⦹㊣สഐ㓴ᴹаᇊ䍑⥞DŽ䘉Ӌ ਟ㛢ᙗǃਈᔲᙗ઼⭏ᘱᆖἽ⢙⢩ᙗⲴ࠶䘋㹼ҶỰ傼ˈҏ䙊䗷㘳ਔᆖ䇱ᦞ઼䙊䗷 ࠶ᆀ䚇Րḷ䇠Ⲵ֯⭘䘋㹼ҶỰ傼DŽ 䈤䜭ᴹ䇨ཊн਼Ⲵ⹄ウᣕ䚃᭟ᤱDŽնᱟˈབྷཊᮠⲴ䇱ᦞ᭟ᤱ⦹㊣ᶕ㠚ٷ䘉Ӌ Ҿ໘㾯କ⦹㴰哽˄Galinat, 1988˅DŽ໘㾯କ⦹㴰哽Ⲵสഐ㓴㊫լҾ⦹㊣ˈ᱃Ҿ਼⦹ ㊣ᵲӔˈ䘈ާᴹࠐњ㊫լҾ⦹㊣ⲴἽ⢙⭏ᘱᆖ⢩ᙗˈ䘉Ӌ䇱ᦞ䇱᰾⦹㊣ᶱᴹਟ㜭 䎧ⓀҾ໘㾯କ⦹㴰哽DŽ ݳ 1511 ᒤᆹᗭⴱlj亽ᐎᘇNJ⢙ӗѝᡰࡇޜѝഭቊᰐᇊ䇪DŽޕ㊣օᰦ㻛ᕅ⦹ ⧽⨐〛㻛䇔Ѫᱟᡁഭ⦹㊣Ⲵᴰᰙ䇠䖭˄ѝഭ⢙䚇Ր䍴Ⓚˈ1994˅DŽኡьߌ、䲒 ѫ㕆Ⲵljѝഭ⦹㊣ṭษᆖNJ˄1986 ᒤ˅ѝ䇔Ѫlj亽ᐎᘇNJѝⲴ⧽⨐〛ᓄ儈㋡Ⲵ ањ૱DŽᡁഭᴰᰙ䇠䖭⦹㊣Ⲵਔ㉽ᴹ 1555 ᒤ⋣ইljᐙ৯ᘇNJǃ1551 ᒤ˄᰾హ 䶆 30 ᒤ˅⋣ইlj㽴৯ᘇNJǃ1555 ᒤ⋣ইljᐙ৯ᘇNJǃ1560 ᒤ⭈㚳ljᒣ߹ᓌᘇNJǃ 1563 ᒤljབྷ⨶ᓌᘇNJ઼ 1574 ᒤⲴljӁই䙊ᘇNJㅹDŽа㡜䇔Ѫ⦹㊣ᱟ⭡䱯՟Ӫ Ӿ㾯⨝⢉ᑖࡠ哖࣐ˈ⭡哖࣐㓿ѝӊ㾯ӊՐޕᡁഭⲴ㾯े䜘ˈՐࡠ䱶ⴱ˗ᡆ Ӿ哖࣐Րޕঠᓖ઼ᡁഭ㾯ই䜘ˈ㘼ਾੁަԆൠ४ՐDŽնᱟ⭡Ҿᖃᰦ⋯⎧ൠ४୶ ъਁኅ䗵䙏઼㡚⎧һъॱ࠶ਁ䗮ˈ⦹㊣㓿⭡⎧䐟Րޕьই⋯⎧ൠ४ҏᱟਟ㜭ⲴDŽ ᰙᵏᕅ䘋Ⲵ⦹㊣ᱟ⺜㋂රⲴˈ傜喯ර⦹㊣ᱟ൘ 20 ц㓚 20 ᒤԓԕਾᕅޕⲴDŽ൘
22 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 1760 ᒤԕࡽ⭡Ӂইᒯ㾯аᑖⲴ⺜㋂රൠᯩ૱㓿ケਈ઼䘹ᤙᖒᡀҶ㌟䍘⦹㊣૱ ˄䈖㿱ljѝഭߌъਁኅਢNJ˄ѕз㤡ㅹˈ1992˅˗ljѝഭߌъ、ᆖᢰᵟਢはNJ˄ằ ᇦࣹˈ1989˅˗ljѝഭ⢙ṭษਢはNJ˄ୀᆷˈ1986˅DŽ 1.1.6 ⭞䙊 ⦹㊣൘ 100 ཊњഭᇦᴹ୶ъॆἽDŽѫ㾱Ⲵ⦹㊣⭏ӗഭᱟ㖾ഭǃѝഭǃᐤ㾯ǃ ໘㾯କǃ⌅ഭ઼ঠᓖˈঐޘц⭼⦹㊣ᙫӗ䟿Ⲵ 75%DŽ൘ӊ⍢ˈѝഭⲴ⦹㊣⭏ӗঐ ѫሬൠսDŽѝഭᱟц⭼кӵ⅑Ҿ㖾ഭⲴㅜҼབྷ⦹㊣⭏ӗഭ˄Morris, 1998˅ˈἽ ⦹㊣ѫ㾱ᱟѪҶ㧧ᗇ⦹㊣㊭㋂ˈަབྷཊᮠ⭘ࣘ⢙侢ᯉˈնᱟҏᴹᖃབྷⲴ䜘࠶ ࡦᡀӗ૱⭘Ҿ伏૱ǃ㦟⢙઼ᐕъӗ૱ㅹᒯབྷ亶ฏDŽӾ 2000 ᒤᔰˈ൘⋣ইⴱ઼ ѝഭ㾯ेൠ४Ⲵ 3 њⴱ⦹㊣㻛⭘ᶕᨀ⛬⟳⭘䞂㋮DŽᓄ⭘㤳ത⧠ᐢᢙབྷࡠьेǃ⋣ ेǃኡьǃ⊏㣿ㅹ䇨ཊൠ४DŽ ⦹㊣㊭㋂ѝ㜊ң㓖ঐ 82%ǃ㜊㓖ঐ 12%ǃⳞ ቲ 㓖 ঐ 5%˄ަѝ 2.2%⭡㋇㓔㔤 ᶴᡀ˅ǃቆㄟ㓖ঐ 1%˄Earle et al., 1946; Perry, 1988˅DŽӪ㊫ሩ⦹㊣Ⲵ⎸䍩ᯩᔿѫ 㾱ᱟ࣐ᐕ伏૱ᡆ伏૱ᡀ࠶ˈਚᴹᖸቁⲴ∄ֻⴤ᧕伏⭘㊭㋂˄Watson, 1988˅DŽ⭘Ҿ 伏૱઼ᐕъⴞⲴⲴ⦹㊣㊭㋂བྷཊᮠ˄77%˅䙊䗷⒯⼘㊹ᯩᔿ䘋㹼࣐ᐕDŽ൘㊭㋂⒯ ⼘Ⲵ䗷〻ѝˈ㜊㣭ਟԕ㻛ᨀਆ⭘Ҿ⭏ӗ⦹㊣⋩ˈնᴤѫ㾱Ⲵӗ૱ᱟ⏰㊹DŽ Ӫ㊫伏૱˖ ሶ㜊㣭䜘࠶਼㜊ң࠶ˈ㓿Ῐਟ࠶ࠪ⦹㊣⋩ˈਟ⭘Ҿࡦ䙐Ӫ䙐哴⋩ǃ✩ 䈳⋩઼✔⛨⋩ˈӪ㊫伏⭘DŽ⦹㊣⋩ቭ㇑ਚঐᮤњἽ⢙⋩ᐲ൪Ⲵаሿ䜘࠶ˈնަ ⭘DŽ⦹㊣⋩൘ߧ㯿ᓖлާᴹڕ؍ਜ਼ᴹⲴཊн価઼㜲㛚䞨তާᴹ䟽㾱Ⲵ㩕ޫ઼ 㢟ྭⲴ俉ણǃ㢢⌭ǃっᇊᙗ઼▴ᓖDŽഐѪਜ਼ᴹӊ哫㜲㛚䞨઼㔤⭏㍐ Eˈ㻛䇔Ѫ ᱟа儈㓗Ἵ⢙⋩DŽ䘉㋮ࡦ⋩ 50%ᐖਣ⭘Ҿ✩⛨⋩઼⋉⋩ˈ25%⭘Ҿ⭏ӗӪ 䙐ྦ⋩ˈ25%⭘ҾަԆⴞⲴˈῘਾⲴ㜊㣭ਟԕ⭘侢ᯉDŽ ሶ⦹㊣㜊ң⼘㊹ˈ❦ਾᗳ༴⨶ˈਟԕሶ⏰㊹Ӿ⦹㊣࠶ࠪᶕDŽ㓖 40% Ⲵ⏰㊹Ѫ伏૱ⴤ᧕⎸䍩ᡆ㘵⭘ҾަԆᐕъⴞⲴˈ㓖 60%䖜ॆѪ⭌ણࡲ ˄White and Pollack, 1995˅DŽ⏰㊹ਟԕ䖜ॆᡀཊ⭌ણࡲ઼ਁ䞥ӗ૱ˈवᤜ儈᷌ ㌆⦹㊣㌆⍶઼҉䞷DŽ⦹㊣⏰㊹ҏਟԕѪа伏૱㓴ᡀᡀ࠶ˈྲңࡦ૱઼ߠ◰ǃ 䶒㋺伏૱ǃ✔ࡦ伏૱ǃ⊔ᯉǃ䞡⋩઼⎃⊔ǃ⋉ǃ㚹ǃ઼劬ࡦ૱ǃ㌆઼᷌侞ᯉ ㅹㅹDŽ ࣘ⢙侢ᯉ˖ ㊣㊭㋂䟼ਜ਼ᴹⲴԓ䉒㜭ᱟ⢢⮌侢ᯉ䟼֯⭘Ⲵ䉧⢙ѝᴰ᱃Ҿԓ䉒Ⲵ㜭⦹ 䟿˄Ensminger et al., 1990˅DŽ⦹㊣㊭㋂䟼ᴹབྷ㓖 83%Ⲵ⻣≤ॆਸ⢙ˈԕ⏰㊹ǃᠺ 㚊㌆ǃ㋺㋮ǃ㌆ǃ㓔㔤㍐઼ॺ㓔㔤㍐Ⲵᖒᔿᆈ൘DŽ⻣≤ॆਸ⢙ѝ⏰㊹Ⲵਜ਼䟿ᴰ儈ˈ ⦹㜭ᨀབྷ䜘࠶㜭䟿DŽ㓔㔤वᤜ㓔㔤㍐઼ॺ㓔㔤㍐ˈਟԕ㻛৽࠽ࣘ⢙⎸ॆ੨᭦DŽ ㊣㊭㋂ѝ⋩Ⲵਜ਼䟿བྷ㓖Ѫ 4%ˈ⦹㊣⋩ਜ਼ᴹབྷ䟿Ⲵ 18:2 ӊ哫䞨ˈ䘉ᱟ⥚઼ᇦᗵ 䴰Ⲵཊ㚊н価઼㜲㛚䞨ѻаDŽоަԆ䉧⢙㊭㋂∄ˈ⦹㊣㊭㋂ѝ㳻ⲭ䍘ਜ਼䟿ሩ ⦹䖳վˈ㓖ঐ㊭㋂ᒢ䟽Ⲵ 10%ˈնᱟ⭡Ҿ⦹㊣൘ࣘ⢙侢ᯉѝᡰঐ∄ֻ䖳儈ˈᡰԕ ㊣ᱟ侢ᯉѝḀӋᗵ䴰≘ส䞨ⲴᶕⓀˈྲ⦹㊣㊭㋂ᱟ⭢⺛≘䞨Ⲵ㢟ྭᶕⓀˈնнᱟ 23 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䎆≘䞨઼㢢≘䞨ⲴᶕⓀDŽ⭢⺛≘䞨઼䎆≘䞨ᱟԕ⦹㊣Ѫѫ㾱侢ᯉᡀ࠶Ⲵᇦǃ⥚ ઼ަԆ⢢⮌ѝєњᴰ䟽㾱Ⲵ䲀ࡦᙗ≘ส䞨˄NRC, 2001˅DŽ ⦹㊣䶒ㅻ㊹ǃ⏰㊹઼㫨便ᒢ䞂㌏ˈѪ⒯⌅઼ᒢ⌅⼘㊹Ⲵ࢟ӗ૱ˈᱟ⢢⮌ 侢ᯉⲴ䟽㾱ᡀ࠶DŽ⦹㊣⏰㊹઼䶒ㅻ㊹ᱟ⒯⌅⼘㊹Ⲵ࢟ӗ૱ˈਟ᧪ޕࣘ⢙侢ᯉѝDŽ 䶒ㅻ䟼㳻ⲭ䍘ਜ਼䟿䖳儈˄60%˅ˈᱟ㊫㜑㖇ঌ㍐Ⲵ䟽㾱ᶕⓀDŽᆳ䙊ᑨ⭘Ҿ⢋ǃ劬ǃ ᇦǃᇐ⢙઼ަԆࣘ⢙侢ᯉˈѫ㾱⭘൘ᇦⲴᰕ㋞䟼DŽ⦹㊣⏰㊹˄⒯Ⲵᡆ㘵ᒢ Ⲵ˅ᱟᶱྭⲴ侢ᯉˈަ⏰㊹઼⋩ਜ਼䟿վˈਟ⎸ॆ㓔㔤ਜ਼䟿儈ˈᱟ㳻ⲭ䍘˄20%˅ Ⲵ䟽㾱ᶕⓀˈѫ㾱⭘Ҿྦ⢋઼㚹⢋Ⲵᰕ㋞䟼DŽ 䲔㊭㋂ཆˈ⦹㊣ҏਟԕࡦᡀ䶂䍞侢ᯉˈ⭘侢ޫ൪઼ྦ⢋൪侢லDŽ 1.1.7 ൞ളⲺᓊ⭞߫ ⦹㊣ᰙ൘ 16 ц㓚ቡ⭡⅗⍢Րᮉ༛ᕅ䘋ѝഭˈॱгц㓚Ր䙽ҶѝഭൠDŽⴞ ࡽˈ⦹㊣൘ѝഭⲴ䇨ཊⴱԭ䜭ᴹἽ˄Rozelle and Carter, 2001˅DŽ ⦹㊣൘ѝഭⲴߌъ⭏ӗ㔃ᶴѝ䎧⵰䟽㾱Ⲵ⭘ˈᱟՈ㢟Ⲵ侢ᯉǃ䟽㾱ⲴⲴᐕ ъᯉ઼Ո䍘Ⲵ㋞伏⢙DŽѝഭᱟц⭼кѫ㾱⦹㊣⭏ӗഭѻаDŽ㖾ഭߌъ䜘㔏䇑 ᮠᦞ㺘᰾ˈ2012 ᒤѝഭⲴ⦹㊣ӗ䟿Ѫ 2.08 ӯˈӵ⅑Ҿ㖾ഭ˄2.74 ӯᱟޘ⨳ ㅜҼབྷ⦹㊣⭏ӗഭ˄USDA-NASS, 2013˅DŽ⦹㊣࠶ᐳ൘े㓜 50°̚20°䰤㠚ьे㠣 㾯ইањ⤝䮯Ⲵൠᑖˈवᤜ 14 њⴱǃᐲǃ㠚⋫४DŽѝഭ⦹㊣ᑖਟ࠶Ѫ 6 њӗ४˖ I. ेᯩ᱕⦹㊣४˗II. 哴␞⎧༿⦹㊣४˗III. 㾯ইኡൠ⦹㊣४˗IV. ইᯩш䲥 ⦹㊣४˗V. 㾯े♼Ⓓ⦹㊣४઼ VI. 䶂㯿儈⦹㊣४˄䈖㿱 1.3.1 䜘࠶઼മ 4˅DŽ ަѝˈࡽ 3 њӗ४ঐ⦹㊣ᙫ䶒〟Ⲵ 80%DŽ শਢкˈ⦹㊣অ⤜ᡆоަԆ䉧㊫㋞伏а䎧ࡦѝഭӪௌ伏Ⲵ伏૱˄ྲ⦹ ㊣侬ǃ䶒वǃ㳻㌅ㅹ˅DŽ勌⦹㊣˄वᤜᲞ䙊⦹㊣઼⭌㌟⦹㊣˅ਟⴤ᧕伏⭘DŽ൘䗷 ৫Ⲵ 20 ᒤ䰤ˈ⦹㊣ᡀѪᴰ䟽㾱Ⲵ侢ᯉ⢙ѻаDŽ⦹㊣Ѫᶀᯉਟ⭏ӗ 300 ཊ ᐕъӗ૱ᡆѪࡦ㦟ᯉDŽ⦹㊣Ⲵᴰ㓸⭘䙄ҏ൘䗵䙏ਈॆ˗⦹㊣↓䎺ᶕ䎺ཊൠ ⭘Ҿབྷරޫ⥚൪઼ᇦ൪ˈ㘼нӵӵᱟа㋞伏⢙ᡆ㘵ߌᇦ䲒ޫ⥚ޫ呑Ⲵ侢 ᯉ˄Rozelle and Carter, 2001˅DŽѝഭབྷ㓖 75%Ⲵ⦹㊣⭘Ҿࣘ⢙侢ᯉˈަ։Ⲵ⭘Ҿ Ӫ㊫⎸䍩઼ᐕъ⭘䙄DŽᦞ㔏䇑˄㘏ࠔ㤡઼ᶘᲃݹ, 2006˅ˈѝഭት≁ᒣ൷⇿ḷ߶Ӫ 䟿Ѫ 9.9 ݻ˄㺘 4˅DŽ80 ᒤԓ൘ѝഭⲴᐲ൪㓿⍾᭩䶙઼䈳ᮤޕཙ⦹㊣伏⢙Ⲵᩴ⇿ 䘋Ҷ⮌⢗ъⲴਁኅˈҏഐ↔ᨀ儈Ҷ⦹㊣Ѫ侢׳Tuan and Peng, 2001˅ᶱབྷൠ˄ ᯉⲴ䴰≲䟿˄Rozelle and Carter, 2001˅DŽ 㺘4. ѝഭґት≁⦹㊣伏⢙ᩴޕ䟿
ൠ४ བྷᐲ ѝሿᐲ а㊫ߌᶁ Ҽ㊫ߌᶁ й㊫ߌᶁ ഋ㊫ߌᶁ ᒣ൷ ᩴޕ䟿˄g/Ӫ/d˅ 8.6 8.0 7.1 7.9 19.5 17.3 9.9
ᓭૂ⭕ᘷ⧥ູᱥੜਇ⭕䗽уᖧଃڛ㊱ሯӰ 1.1.8 ݳࡽ 2,700 ᒤቡѪ㋞伏⢙䘋㹼ἽҶޜ㊣ӗҾ໘㾯କˈᰙ൘⦹ ˄Salvador, 1997˅DŽࠐⲮᒤᶕˈаⴤᱟࣘ⢙侢ᯉ઼Ӫ㊫Ⲵѫ伏DŽ⦹㊣㊭㋂৺ަ࣐ ᐕᡀ࠶ਟԕ⭘൘伏૱઼ࣘ⢙侢ᯉӗ૱䟼DŽ伏⭘⦹㊣ᰦਁ⭏Ⲵ䗷৽ᓄᶱѪቁ 24 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㿱˗⹄ウᴰཊⲴᱟ਼㜲㊫Ր䙂㳻ⲭ䍘ᴹޣⲴ䈮仈˄Pastorello et al., 2000˅DŽ↔ཆˈ ⦹㊣㊭㋂ѝнਜ਼∂㍐઼㠤㳻ⲭ˄Watson, 1982; White and Pollak, 1995˅DŽ ቭ㇑⦹㊣൘ޘц⭼ᒯ⌋ἽˈնᱟӪԜᒦн䇔ѪᆳՊᡀѪ≨ѵᙗᵲ㥹ᡆ㘵䳮 Ҿ䱢⋫Ⲵᵲ㥹DŽ↓ྲᡁԜӺཙᡰ⸕ˈ⭡Ҿ⦹㊣䳼ᙗ㣡ᒿ˄᷌ょ˅㜭䲀ࡦᆀⲴՐ ˈഐ↔⦹㊣н㜭൘䟾⭏⧟ຳ䟼ᆈ⍫DŽ 䗴ӺѪ→ˈἽ⦹㊣ǃ伏⭘⦹㊣˄वᤜ伏⭘ਜ਼ᴹ⦹㊣ᡀ࠶Ⲵ伏૱˅ǃѪ侢 ᓧ৺⭏ᘱ⧟ຳӗ⭏䗷н࡙ᖡ૽Ⲵᣕڕᯉᡆ⭘Ҿᐕъ⭘䙄ˈ䜭ᵚ㿱ሩӪ㊫ᡆࣘ⢙Ⲵ 䚃DŽ 1.1.9 Ԅ়ਨрⵁθ։ὃ⢟╊Җᡆᴿᇩὃ⢟δྸᵸ㥿ㅿεⲺਥ㜳ᙝ ⦹㊣ᱟ䎧ⓀҾ㾯ॺ⨳ѪᮠнཊⲴѫ㾱⢙⢙ѻаDŽ㲭❦ⴞࡽ䘈⋑ᴹࡠ ޜ䇔Ⲵ⦹㊣䎧Ⓚˈնᶱᴹਟ㜭໘㾯କ⦹㴰哽൘⦹㊣Ⲵ䚇Ր㛼Ჟ䟼䎧Ҷ䟽㾱Ⲵ ⭘DŽ⦹㊣Ӿ䟾⭏⢙傟ॆᡀ䶐Ӫ㊫㙅㜭⭏ᆈⲴṭษ⢙ˈ㓿শҶᖸ䮯Ⲵ ᰦ䰤DŽ↓ྲᡁԜӺཙᡰ⸕ˈоᵲ㥹н਼ˈ⭡Ҿ⦹㊣䳼ᙗ㣡ᒿ˄᷌ょ˅㜭䲀ࡦ DŽੁٮᆀⲴՐˈ⦹㊣н㜭൘䟾⭏⧟ຳ䟼ᆈ⍫ˈഐ↔ṭษ⦹㊣нާᴹᵲ㥹 1.1.10 ᱥੜᴿ䮵ᵕᆿޞᓊ⭞Ⲻ䇦ᖋ ݳࡽ 2700 ᒤቡѪ㋞伏⢙䘋㹼ޜࡽᡰ䘠ˈ⦹㊣ӗҾ໘㾯କˈᰙ൘ྲ ἽҶ˄Salvador, 1997˅DŽ䮯ᵏԕᶕˈ⦹㊣аⴤᱟӪ㊫઼ࣘ⢙Ⲵѫ㾱伏⢙ᶕⓀˈ ⭘শਢDŽ֯ޘᆳ⭘Ҿ伏૱ާᴹ䮯ᵏⲴᆹ 1.2 ։ὃ⢟Ⲻ⭕⢟ᆜ⢯ᙝ 1.2.1 жᒪ⭕䘎ᱥཐᒪ⭕ ⦹㊣ᱟ䳼䳴਼Ṛᔲ㣡Ⲵаᒤ⭏ᵜ、ṭษἽ⢙ˈ䴰㾱Ӫᐕ䖵ࣙਟՐ ᆀԕᆼᡀ㑱⇆઼⭏ᆈ˄OECDˈ2003˅DŽ 1.2.2 ሯӰ㊱ެᆹ⭕⢟ᱥੜᴿ∈θྸᴿ∈θᓊ䈪᱄∈ᙝᆎ൞Ⲻ䜞փ ެ∈ᙝⲺᙝ䍞 ṩᦞ OECD˄2002˅ˈ⦹㊣䟼ᴹࠐњӪԜ⟏⸕Ⲵᣇ㩕ޫഐᆀˈवᤜἽ䞨ǃ бᐳ˄2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3 (4H)-one (DIMBOA)˅ǃỹ ᆀ㌆ǃ㜠㳻ⲭ䞦ᣁࡦࡲ઼㜠ࠍң㳻ⲭ䞦ᣁࡦࡲDŽ ㊣㊭㋂ѝⲴἽ䞨㜭㷟ਸⸯ⢙㩕ޫݳ㍐ˈवᤜ䫉ǃ䭱ǃ䫮ǃ䫱઼䬼ˈ֯অ⦹ 㛳ࣘ⢙൘⭏⨶ᆖкн㜭⎸ॆ੨᭦䘉Ӌ㩕ޫݳ㍐˄Liener, 2000˅DŽӪԜ਼ᰦ䇔Ѫ Ἵ䞨ሩҾࣘ⢙ˈ⢩࡛ᱟ䶎৽࠽ࣘ⢙ˈᱟа䟽㾱Ⲵᣇ㩕ޫഐᆀˈഐѪᆳ㜭䱽վ ⼧Ⲵ⭏⢙ᆖᴹ᭸ᙗDŽഐ↔൘侢ᯉ࣐ᐕ䗷〻ѝӪԜ䙊ᑨՊੁ⥚઼ᇦ侢ᯉѝ仍ཆ ࣐Ἵ䞨䞦ˈԕᨀ儈⼧Ⲵ࡙⭘⦷DŽ бᐳ˄DIMBOA˅Ҿа㓴ԓ䉒⢙ˈণ≗㛏䞨઼䞊㊫ॆਸ⢙ˈ䙊ᑨਟԕ൘ 䉧㊫Ἵ⢙ѝਁ⧠DŽDIMBOA 䟼Ⲵ㪑㨴㌆㤧ণ˖DIMBOA-glcˈᱟ൘⦹㊣ᴰࡍਁ
25 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㛢䱦⇥ᆈ൘Ҿൠк䜘㔯㢢㓴㓷઼ṩ䜘㓴㓷䟼Ⲵ䘉㊫ॆਸ⢙ѝᴰ䟽㾱Ⲵа ˄Cambier et al., 2000˅DŽDIMBOA-glc ൘ਇՔⲴἽ⢙㓴㓷䟼ˈ࡙⭘䞦৫䲔㪑㨴 ㌆㤧ˈਈᡀ DIMBOAˈਾ㘵ሩᰶ㲛ᴹ∂ᙗ˄OECD, 2002˅DŽ ỹᆀ㌆ᱟаվ࠶ᆀ䟿Ⲵ⻣≤ॆਸ⢙ˈ⭡Ҿ伏⭘ਾᆳ㜭ӗ⭏≄փ䙐ᡀ㛐㛳 ≄㛰ˈӪԜ䇔Ѫᆳᱟаᣇ㩕ޫഐᆀ˄Maynard et al., 1979˅DŽ ↔ཆˈ⦹㊣䟼䘈ᴹᖸቁ䟿Ⲵ㜠㳻ⲭ䞦઼㜠ࠍ㳻ⲭ䞦Ⲵᣁࡦഐᆀˈн䗷䙊ᑨ ӪԜ䇔Ѫ䘉Ҽњഐᆀ䜭нՊሩ㩕ޫӗ⭏䟽㾱ᖡ૽˄White and Pollak, 1995˅DŽ 䲔Ҷ䘉Ӌᣇ㩕ޫഐᆀཆˈ⋑ᴹᣕ䚃㺘᰾⦹㊣ѝਜ਼ᴹަᆳᴹ∂⢙䍘DŽ 1.2.3 ᱥੜᴿ㠪ᮅθྸᴿθᓊ䈪᱄㠪ᮅᆎ൞Ⲻ䜞փެ㠪ᮅ⢯ᙝ ⦹㊣䮯ᵏѪӪ㊫઼ࣘ⢙Ⲵ伏⢙ˈ䙊ᑨҾ䶎䗷ᙗ伏⢙DŽⴞࡽᖸቁᴹ ޣ伏⭘⦹㊣ӗ૱ਾਁ⭏䗷৽ᓄⲴᣕ䚃˄OECD, 2002˅DŽ䗷৫ॱࠐᒤѝˈ൘㖾 ᣕ䚃˄Pauls and Cross, 1998; Tanaka et al., 2001˅DŽޣഭਚᴹєֻ Jones et al.˄1995˅䇔Ѫˈ䇨ཊሩ㊭㋂㺘⧠䗷ⲴӪᇎ䱵кᱟሩ㣡㊹䗷ˈ 䘉ӋӪѝᴹབྷ㓖 80%ⲴӪ䘋㹼伏⢙᭫ࠫ䈅傼ᰦ⋑ᴹӗ⭏Ѥᒺ䗷⯷⣦DŽ↔ཆˈ ൘ᴮ㓿ሩ㊭㋂ӗ⭏䗷৽ᓄⲴӪѝˈ㲭❦ཊᮠՊሩሿ哖㳻ⲭ䍘ਁ⭏䗷৽ᓄ ˄~75%˅ˈնሩ⦹㊣㊭㋂ӗ⭏䗷৽ᓄⲴӪᶱቁˈаⲮཊս⯵Ӫѝнࡠ 6 սੁ 䈕ሿݯ、䗷৽ᓄу、ѝᗳᣕਁ⭏Ҷ䗷৽ᓄDŽ ᶕ㠚Ҿབྷ࡙Ⲵєԭᣕ˄Pastorello et al., 2000; Pasini et al., 2002˅㺘᰾ˈ ᴹаӋ䗷⯵Ӫࠪ⧠Ⲵ伏૱䗷৽ᓄˈ਼伏⭘वᤜ⦹㊣㋕൘Ⲵ⦹㊣ӗ૱ࠪ⧠ Ⲵ䗷৽ᓄ⯷⣦а㠤ˈަѝᶕ㠚⌒䟼Ⲵ 6 ս⯵Ӫ䖫ᓖ伏૱᭫ࠫ䈅傼㔃᷌ 䱣ᙗDŽնᱟˈк䘠བྷ࡙⹄ウѝᡰᣕⲴ⯵Ӫˈ䙊ᑨሩᵲ㥹ǃprunidae ㊫Ἵ⢙ ӗ⭏Ⲵ㣡㊹ǃ㊭㋂৺俉ᯉㅹˈ䜭Պਁ⭏ཊ䟽䗷৽ᓄDŽཊ䟽䗷৽ᓄ֯ᗇѤᒺ к䇶࡛⢩ᇊ⯵ֻⲴ㠤ᰦާᴹн⺞ᇊᙗ˗⭡Ҿ䗷৽ᓄѻ䰤ᡆཊᡆቁ▌൘Ⲵ Ӕ৹⭘ˈ֯ᗇⳞ䈅䇺ᯝ઼փ IgE 㔃ਸ䈅傼ᴤѪ༽ᵲ˄Pastorello et al., 2000˅DŽ ⦹㊣㊭㋂ѝѫ㾱㠤ѻаѪ࠶ᆀ䟿 9kDaⲴ㜲䖜䘀㳻ⲭ˄LTPˈlipid transfer protein˅ˈҾ伏⢙ѫ㾱䗷ॱޛ䟋ӊᇦ᯿ˈо≤にǃṳᆀоབྷ哖ㅹ䗷਼ Ⓚˈ⋑ᴹਁ⧠⦹㊣⢩ᔲⲴ LTP 䗷˄Pastorello et al.ˈ2000˅DŽ⦹㊣оަԆἽ ⢙㣡㊹䗷ޣᡀԭሩ∄࠶᷀⋑ᴹਁ⧠⦹㊣⢩ᔲⲴ㠤˄Petersen A., 2006˅DŽⴞࡽˈ䘈⋑ᴹ䏣ཏⲴ䇱ᦞᶕҶ䀓൘བྷ࡙ᡆൠѝ⎧ൠ४⦹㊣ӗ૱䗷৽ ᓄ⍱㹼Ⲵ⢩⛩ˈҏ䘈⋑ᴹᶕ㠚Ҿц⭼ަԆൠ४ޣҾ⦹㊣伏૱䗷৽ᓄⲴᣕᇎ DŽ䘉㺘᰾ˈ⭡Ҿ伏⭘⦹㊣৺ަ㹽⭏ӗ૱ˈሩӪ㊫䙐ᡀ䗷৽ᓄ仾䲙Ⲵਟ㜭ᙗֻ ᖸվ˄Scibilia et al., 2008˅DŽ 1.2.4 㑷⇌ᯯᕅᱥᴿᙝ㑷⇌䘎ᱥᰖᙝ㑷⇌θྸѰᴿᙝ㑷⇌θᱥ㠠㣧ᦾ ㊿䘎ᱥᔸ㣧ᦾ㊿ᡌᑮᔸ㣧ᦾ㊿χᱥ㲡Ֆ㊿䘎ᱥ伄Ֆ㊿ ⦹㊣ᱟᴹᙗ㑱⇆⢙ˈᱟ㠚ӔǃᵲӔ൷ӢਸⲴ⢙˄Wilkes, 1972, 1989˅DŽ 㣡㊹ⲴՐᯩᔿѫ㾱ᱟ仾ჂՐ㊹˄OECD, 2003b˅ˈ㲛ჂՐ㊹䖳ቁDŽ㠚㣡ᦸ㊹ аἽṚ䚇Ր⢩ᖱ䎻ੁ㓟ਸˈ㘼ᔲ㣡ᦸ㊹㜭㔃ਸн਼ἽṚⲴ䚇Ր⢩⛩ˈ䘉਼֯
26 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 а㠚ӔǃᵲӔⲴᾲᘥ઼⭡↔ӗ⭏Ⲵӗ䟿᭸ᓄᱟ⧠ԓ⦹㊣ᵲӔ㛢Ⲵ⨶䇪สDŽ ᴹ⭏ભ࣋Ⲵ㣡㊹ਟԕՐⲴ䐍ਆߣҾ仾Ⲵ⢩⛩ǃ⒯ᓖ઼ᓖㅹDŽ 1.2.5 ൞㠠❬ᶗԬсфੂ〃ᡌ䘇㕎〃ⲺᔸӚ⦽ ⦹㊣ᱟ仾Ⴢᦸ㊹ǃ㠚ӔӢਸǃᵲӔਟ㛢Ⲵ⢙˄Wilkes, 1972, 1989˅DŽᴹ⭏ ભ࣋Ⲵ㣡㊹ਟԕՐⲴ䐍ਆߣҾ仾Ⲵ⢩⛩ǃ⒯ᓖ઼ᓖDŽ䲔ҶḀӋ⠶㻲⦹㊣૱ ઼ᵲӔഐѪањ䝽ᆀփ䚇Րഐᆀ˄൘ḃ㢢փ 4 кⲴ GasǃGa ઼ ga ㅹսสഐ ㌫ࡇ˅ᖡ૽ަਇ㋮ԕཆˈ⦹㊣о䘁㕈䰤ਟԕ䘋㹼ᵲӔᦸ㊹DŽ ⦹㊣઼໘㾯କ⦹㴰哽ާᴹ䚇ՐਟӢਸᙗDŽ൘໘㾯କ઼ডൠ傜ˈᆳԜ൘䐍 䖳䘁ǃަᆳᶑԦ㢟ྭᰦˈਟԕ㠚⭡ᵲӔDŽն໘㾯କ⦹㴰哽Ⲵ㠚❦࠶ᐳӵተ䲀Ҿ⋯ ⵰໘㾯କ઼ডൠ傜㾯䜘ፆඑⲴᆓ㢲ᙗᒢ⠕Ⲵǃ༿ᆓᴹ䴘Ⲵӊ✝ᑖൠ४઼໘㾯କ ѝ䜘儈ൠ४˄Wilkes, 1972; Gonzalez and Corral, 1997˅DŽӪᐕ䟷䳶໘㾯କ⦹㴰哽 㣡㊹㔉⦹㊣ᵲӔ઼ᔰ᭮ᦸ㊹㗔փᦸ㊹ˈ⇿ょᒣ൷ਟԕ࠶࡛ᗇࡠ 8 ㋂઼ 12 ㋂ ᆀˈ㔃ᇎ⦷㓖Ѫ 1-2%˄Baltazar et al., 2005˅DŽ৽䗷ᶕˈ⭘⦹㊣㣡㊹㔉໘㾯କ⦹㴰 哽ᦸ㊹㔃ᇎቡՊᖃഠ䳮ˈ⇿ょ㔃ᇎᴰཊҏቡᱟ 0.2-0.3 ㋂˄Baltazar et al., 2005; Ellstrand et al., 2007˅DŽ ⦹㊣઼᪙ᬖਟᵲӔˈնᶱѪн᱃DŽᒦфᵲӔާᴹ儈ᓖⲴн㛢ᙗˈ൘䚇 Րᆖкҏнっᇊ˄Mangelsdorf, 1974˅DŽGalinat˄1988˅Ⲵ⹄ウ䘋а↕䈤᰾ˈ⭡Ҿ ᪙ᬖ઼⦹㊣ḃ㢢փᮠⴞн਼ˈ࣐ањཊ։Ⲵ᪙ᬖḃ㢢փࡠ⦹㊣สഐ㓴 Ⲵࠐ⦷ᱟᖸվⲴˈഐ↔ḃ㢢փ䰤ⲴӔᦒ⦷ᶱվDŽ 1.2.6 㛨ᙝδਥ㛨䘎ᱥу㛨θ㛨ᙝ儎քθྸ᷒у㛨θᓊ䈪᱄ኔ〃у 㛨㊱ශε ਇփ⦹㊣ਟ㛢ˈ൘㠚❦ᶑԦлާᴹᦸ㊹ǃਇ㋮઼㔃ᇎ㜭࣋DŽնᴹ䇨ཊഐ㍐ ਟԕᕅ䎧⦹㊣Ⲵ䳴㣡н㛢ˈྲ儈ǃᒢᰡǃ䗀ሴǃॆᆖ㦟⢙༴⨶ԕ৺㩕ޫݳ㍐ 㕪ѿㅹDŽ 1.2.7 ޞ⭕㛨ᵕ ᓖ઼⭏䮯ਁ㛢Ⲵޣ㌫˖ ⦹㊣ᱟаᒤ⭏Ἵ⢙ˈަ⭏㛢ᵏⲴ䮯⸝ਆߣҾ૱⢩ᙗ৺ަ⭏䮯Ⲵ⧟ຳDŽ⭏䮯 ⛩䵢ࠪൠ䶒˄5 ⡷ኅᔰਦࡠ 7 ⡷ኅᔰਦ䱦⇥˅ԕਾˈ⦹㊣൘վҾ 0°C Ⲵᓖлᆈ ⍫ᰦ䰤н䎵䗷 6~8 ሿᰦˈ߫ᇣ〻ᓖਆߣҾ 0°C ԕлᓖᤱ㔝Ⲵᰦ䰤䮯⸝ǃ൏༔ᶑ Ԧǃ↻㥜ǃվᵏⲴ䮯⸝ǃ仾ੁǃሩ⒯ᓖ઼Ἵ⢙ਁ㛢䱦⇥DŽ൘ᑖൠ४᱕ᰦ Ⲵ䖫䵌߫ਟԕᕅ䎧ਦ⡷ᦏՔˈҏᖡ૽⦹㊣♼⍶DŽഐ↔ˈ⦹㊣㜭ᆼᡀ⭏ભઘᵏਆ ߣҾᰐ䵌ᵏⲴ䮯⸝DŽа㡜ᰙ⟏૱ࡠ⟏૱ޘ⭏㛢ᵏ㓖Ӿ 90 ཙ~ 130 ཙнㅹDŽ ᰐ䵌ᵏⲴ䮯⸝ߣᇊҶн਼Ⲵ⦹㊣૱Ⲵ⭏㛢ᵏ䮯⸝н਼DŽᓄⲴᡀ⟏ᵏҏਆ ᶑԦǃൠᖒǃབྷර≤փ઼൏༔㊫ර˄Troyer, 1994˅DŽى≄ߣҾ⭏㛢ᵏ䰤Ⲵ ਼а૱Ⲵᡀ⟏ᵏ൘਼аᒤн਼ⲴἽൠ⛩ᴹᡰн਼ˈ൘਼аൠ⛩н਼ᒤԭ ҏ〽ᴹн਼ˈ䘉ਆߣҾӾἽࡠ᭦㧧㓿শⲴ⧟ຳᶑԦDŽ 27 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⭏䮯ਁ㛢䱦⇥˖ ⦹㊣㠚㠣ᡀ⟏㓿শҶ㩕ޫ⭏䮯˄V˅઼⭏⇆⭏䮯˄R˅䱦⇥˄Kumudini and Tollenaar, 1998˅DŽ䘉њ䗷〻ਟ䘋а↕࠶Ѫ䇨ཊ䱦⇥˄മ 4˅˖
മ4. ⦹㊣⭏䮯ਁ㛢䱦⇥⽪മ 㤇ᵏ˄VE~V5˅˖⭡Ҿѝ㜊䖤䗵䙏⭏䮯ˈ⦹㊣ࠪ൏DŽ䲿ਾˈ㜊ਦᔰ䙊䗷㜊 㣭䷈ቆ䮯ࠪᶕˈ50%ԕк⦹㊣㤇儈 2~3 cm ԕк〠Ѫࠪ㤇DŽ㤇ᵏᱟԕ⭏ṩǃ䮯ਦǃ 㤾㢲࠶ॆѪѫⲴ㩕ޫ⭏䮯䱦⇥ˈа㡜শᰦ 20~35 ཙнㅹDŽ ᤄ㢲ᵏ㠣ᣭ䳴ᵏ˄V6~VT˅˖⦹㊣⭏䮯⛩սҾൠ䶒ԕкˈ㤾䗵䙏ը䮯֯⦹㊣ ᤄ㢲DŽᤄ㢲ᱟ⭡⦹㊣⭡㩕ޫ⭏䮯䖜ੁ㩕ޫ⭏䮯о⭏⇆⭏䮯ᒦ䘋ⲴᰦᵏDŽ㠣ᴰਾа ⡷ਦ䮯ࠪˈ䲿ਾ䳴ょࠪ⧠ˈ⦹㊣䗮ࡠᴰབྷṚ儈DŽа㡜শᰦ 27~30 ཙDŽ ੀэᵏ˄R1˅˖ᖃ㣡э൘㤎ਦཆ䶒ਟ㿱ˈᣭэণᔰˈ൘䘉а䱦⇥㜊⨐ཆ 㿲ⲭ㢢ˈ䜘Რਟ㿱ˈਜ਼ᴹᖸቁⲴ⏢փˈ㜊нਟ㿱DŽ൘ᣭэࡽਾⲴҼઘᱟ⦹㊣ ἽṚⲴ⭏ભઘᵏ䟼ሩ⧟ຳ䘶ຳᴰᝏⲴ䱦⇥DŽ൘䘉а䱦⇥ˈ൘䘶ຳᶑԦлᑨ㿱㊭ ㋂䍕㛢DŽ䍕㛢䙐ᡀⲴ㊭㋂ᮠⴞⲴ߿ቁՊ䙐ᡀѕ䟽߿ӗDŽ ≤⌑ᵏ˄R2˅˖ੀэਾབྷ㓖 10~14 ཙˈ≤⌑ᵏণᔰDŽަ⢩⛩ᱟ㊭㋂Ⲵょ䖤 кᴹ␑ⲭ㢢Ⲵ≤⌑⣦㊭㋂ˈ䟼䶒Ⲵ㜊ңਜ਼ᴹ䘿᰾Ⲵ⏢փˈ㘼㜊ᖒ⣦䖳ሿնਟԕ࠶ 䗘ࠪᶕDŽ㣡ᐢ㓿ᆼᡀҶᦸ㊹ਇ㋮࣏㜭ˈᔰᒢᷟDŽ ң⟏ᵏ˄R3˅˖㣡эᖒᡀਾབྷ㓖 18~22 ཙˈ㊭㋂ཆ㿲Ѫ哴㢢ˈ䲿⵰⏰㊹൘㜊 ң䟼ᔰ〟㍟ˈަѪң⣦ⲭ㢢⏢փDŽ↔ᰦ䙊䗷䀓ࢆˈ㜊᱃Ҿ䗘䇔DŽң⟏ᵏょ㋂ ᮠᐢ㓿⺞ᇊˈ㊭㋂ѝᒢ⢙䍘Ⲵ〟㍟ᱟᖡ૽ӗ䟿Ⲵѫ㾱ഐ㍐DŽ ֯ˈ㵑⟏ᵏ˄R4˅˖㣡эᖒᡀਾབྷ㓖 24~28 ཙˈ䲿⵰⏰㊹൘㜊ңᤱ㔝〟㍟ ㊭㋂䜘ң⣦⏢փਈぐˈᖒᡀ“ഒṧ”⏢փˈ㵑⟏䱦⇥ণᔰDŽ ࠩ䲧ᵏ˄R5˅˖㣡эᖒᡀਾབྷ㓖 35~42 ཙˈޘ䜘ᡆ䜘࠶㊭㋂亦䜘ᔰࠩ䲧ˈ ൘㊭㋂Ⲵࠩ䲧ㄟ䱴䘁ˈࠪ⧠Ҷаᶑ᰾ᱮⲴ≤ᒣ㓯ˈᆳᱟᡀ⟏㊭㋂⏢փ˄ң˅४઼ പփ˄⏰㊹˅४Ⲵ࠶⭼㓯ˈ〠“ң㓯”DŽ䲿⵰㊭㋂Ⲵᡀ⟏ˈ“ң㓯”ሶੁ⵰㊭㋂Ⲵ ส䜘˄ょ䖤ᯩੁ˅〫ࣘDŽᖃң㓯൘㊭㋂Ⲵ亦䜘઼ส䜘ѻ䰤䗮ࡠ 50%ᰦˈ㊭㋂ণѪ 40~45%ਜ਼≤䟿ˈᒦфᐢ㓿䗮ࡠҶަᴰ㓸ᒢ⢙䍘䟽Ⲵ 95%DŽ ㊭㋂ˈᆼ⟏ᵏ˄R6˅˖㣡эᖒᡀਾབྷ㓖 55~65 ཙˈ㊭㋂Ⲵᒢ⢙䍘䗮ࡠᴰབྷ٬ ণ䗮ࡠ⭏⨶ᙗᡀ⟏DŽ൘㊭㋂Ⲵң㓯⎸ཡਾнѵˈ㊭㋂ส䜘ᖒᡀ唁ቲˈቡ䗮ࡠҶᆼ 28 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⟏ᵏ䱦⇥DŽ唁ቲ俆ݸ൘᷌ょ亦䜘㊭㋂ࠪ⧠ˈ䲿ਾส䜘㊭㋂䙀↕ӗ⭏DŽ唁ቲࠪ⧠ਾ ㊭㋂Ⲵᒣ൷ਜ਼≤䟿Ѫ 30~35%ˈնਟԕ䲿⵰⧟ຳᶑԦⲴн਼㘼ᴹᡰਈॆDŽ 1.2.8 ൞㠠❬⮂ѣ⭕ᆎ㑷⇌Ⲻ㜳࣑θऻᤢ䏀ߢᙝȽ䏀ཅᙝᣍ䘼ᙝㅿ ㊣㓿䗷䮯ᵏⲴ傟ॆˈ֯ᆀᡀѪୟа㑱⇆ಘᇈˈᒦ䴰㾱Ӫ㊫Ⲵᑞࣙ֯ަ㔗⦹ 㔝цԓ㑱㹽ᡆ㘵オ䰤Ր˄Gould, 1968; OECD, 2002˅DŽ൘㠚❦⭏ຳ䟼ˈ⦹㊣ᱟ 㻝ᙗⲴ⢙ˈᒦфᐢ㓿їཡҶަ൘䟾⭏ᶑԦлᆈ⍫Ⲵ㜭࣋˄Gould, 1968˅DŽץ䶎 ਼ᵲ㥹Ἵ⢙∄ˈ⦹㊣ᴹ䳼ᙗ㣡ᒿ˄᷌ょ˅ˈょ䖤㻛㤎ਦव㼩DŽҾᱟˈа㡜н㜭 ਁ⭏অ㋂ᆀⲴՐDŽ ⦹㊣нާᰐᙗ㑱⇆㜭࣋ˈᆀнާՁⵐ⢩ᙗDŽ⦹㊣ᆀⲴᆈ⍫ਆߣҾᓖǃ ↔ᣔ઼ᆀᡀ⟏ᓖㅹDŽᓖ䱽ࡠ 0°C ԕлՊᖡ૽㨼ਁˈഐ؍⒯ᓖǃสഐරǃ᷌Ⳟ վᱟᆀ⭏ӗᵏ䰤Ⲵѫ㾱仾䲙DŽ儈Ҿ 45°C ⲴᓖҏՊሩᆀⲴ⭏⍫࣋ӗ⭏н 㢟ᖡ૽˄Shaw, 1988˅DŽ≤࠶൘⦹㊣Ⲵ⭏䮯ᆓ㢲ᱟ䲀ࡦᙗഐ㍐ˈቔަ൘⦹㊣Ⲵᔰ 㣡ᵏ઼♼⍶ᵏˈ≤࠶н䏣ਟԕሬ㠤ᦸ㊹ഠ䳮ᡆ㜊㛾䍕㛢˄Campos et al., 2006˅DŽ 㲭❦൘ка⭏䮯ᆓ㢲⍂㩭Ⲵ⦹㊣ਟԕ䎺ߜᒦᴹਟ㜭൘лᒤ㨼ਁˈնᱟᆳн㜭 ↔ᵲ㥹аṧ䮯ᵏᆈ⍫DŽ൘䇨ཊߌ⭠㌫㔏䟼ˈᑨ㿱Ⲵ⦹㊣㠚⭏㤇ᖸᇩ᱃৫䲔DŽഐۿ ᔰӪᐕṭษ㇑⨶ˈ⦹㊣н㜭䮯ᵏᤱ㔝㑱⇆˄Gould, 1968˅DŽ 1.3 ։ὃ⢟Ⲻ⭕ᘷ⧥ູ 1.3.1 ൞ളⲺ൦⨼࠼ᐹૂ㠠❬⭕ູ ⦹㊣൘ѝഭ࠶ᐳᖸᒯˈই䎧⎧ইዋˈेࡠे㓜 50°Ⲵ唁嗉⊏ˈь䎧ਠ⒮৺⋯ ⎧ⴱˈ㾯ࡠᯠ⮶ǃ䶂㯿儈ˈ䜭ᴹ⦹㊣ἽDŽьे৺㾯ই儈ሂኡ४Ѫ᱕⦹㊣ˈ ᶑԦǃى≄哴␞⎧⍱ฏѪ༿⦹㊣ˈ൘ᒯ㾯ǃ⎧ইㅹⴱਟаᒤєᆓἽDŽṩᦞൠ ⭏ӗᶑԦ઼ἽࡦᓖˈӾьेࡠ㾯ইⲴ⤝䮯४ฏˈᖒᡀҶѝഭ⦹㊣Ⲵѫ㾱Ἵ ४˄മ 5˅ˈণेᯩ᱕⦹㊣४ǃ哴␞⎧༿⦹㊣४ǃ㾯ই⦹㊣४ǃইᯩ⦹㊣४઼㾯 े⦹㊣४DŽ ेᯩ᱕⦹㊣४˖Ѫᡁഭѫ㾱⦹㊣ѫӗ४ˈঐޘഭᙫ䶒〟Ⲵ 39.2%ˈঐޘ ഭᙫӗⲴ 43.8%DŽ䈕४वᤜ唁嗉⊏ǃਹ᷇ǃ䗭ᆱǃᆱ༿ˈ㫉ਔǃኡ㾯བྷ䜘ˈ⋣ ेǃ䲅㾯઼⭈㚳े䜘DŽ⭏ᘱᶑԦ઼⭏ӗᶑԦ䜭䘲ਸ⦹㊣⭏䮯ˈൠ࣯ᒣඖˈ൏༔㛕 ݹ➗ݵ䏣ˈᰐ䵌ᵏ 130~170 ཙˈᒤ䱽䴘䟿 400~800 mm˄60%䱽䴘൘ 6~9 ᴸˈ⊲ 䰤˅DŽҾаᒤа⟏ࡦൠ४DŽेᯩ᱕⦹㊣४ѫ㾱वਜ਼˖ेᯩᶱᰙ⟏᱕⦹㊣㊫ර४ǃ ेᯩᰙ⟏᱕⦹㊣㊫ර४ǃьॾेѝᰙ⟏᱕⦹㊣㊫ර४ǃьॾेѝ⟏᱕⦹㊣㊫ර४ ઼ьॾेѝ⟏᱕⦹㊣㊫ර४ㅹӄњ⦹㊣઼䶂䍞⦹㊣㊫ර४ˈԕ৺ेᯩ勌伏⭌⦹ ㊣ǃ勌伏㌟⦹㊣㊫ර४઼⠶㻲⦹㊣㊫ර४DŽ 哴␞⎧༿⦹㊣४˖Ѫᡁഭ⦹㊣Ⲵѫӗ४ˈঐޘഭᙫ䶒〟Ⲵ 32.7%ˈঐޘ ഭᙫӗⲴ 35.5%DŽ䈕४वᤜኡь઼⋣ইǃ⋣ेབྷ䜘ǃኡ㾯ѝই䜘ǃ䲅㾯ޣѝൠ४ ઼⊏㣿ᗀ␞ൠ४ˈᱟ⦹㊣Ⲵ䳶ѝӗ४DŽ≤䍴ⓀѠᇼˈ哴⋣ǃ␞⋣઼⎧⋣⁚ク䈕४ˈ ᒤ䱽䴘䟿Ѫ 500~900 mm˄70%䱽䴘൘༿ᆓ˅ˈ♼Ⓓ䶒〟䗮 50%ˈҾаᒤє⟏ࡦ ൠ४DŽ哴␞⎧༿⦹㊣४ѫ㾱वਜ਼˖哴␞⎧༿⦹㊣㊫ර४ǃӜ⍕ްᰙ⟏༿⦹㊣㊫ර
29 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ४ㅹєњᲞ䙊⦹㊣઼䶂䍞⦹㊣㊫ර४ˈԕ৺哴␞⎧勌伏⭌⦹㊣ǃ勌伏㌟⦹㊣㊫ර ४઼⠶㻲⦹㊣㊫ර४DŽ 㾯ই⦹㊣४˖वᤜമ 5 ѝⲴ㾯ইኡൠш䲥⦹㊣४઼䶂㯿儈⦹㊣४єњӊ४DŽ ަѝ䶂㯿儈⦹㊣४սҾ儈㓜ᓖൠ४ˈवᤜ䶂⎧ⴱ઼㾯㯿㠚⋫४ǃഋᐍ㾯䜘઼Ӂ ইे䜘ൠ४DŽ⦹㊣ṭษশਢ䖳⸝ˈ䶒〟ᖸሿDŽ㾯ইኡൠ⦹㊣४वᤜഋᐍǃӁ ই઼䍥ᐎⴱǃ䲅㾯ই䜘ǃᒯ㾯㾯䜘儈ൠ४ǃ⒆ইǃ⒆े઼⭈㚳䜘࠶ൠ४DŽኡൠ ঐ䈕४Ⲵ 90%ˈൠᖒ༽ᵲDŽᰐ䵌ᵏѪ 240~330 ཙˈ䱽䴘䟿Ѫ 800~1200 mmDŽ䈕 ४ἽⲴ⦹㊣᱃ਇ⭏⢙઼䶎⭏⢙㛱䘛˄ྲᒢᰡ઼⯵㲛ᇣ˅ⲴডᇣDŽҾаᒤа⟏ ᡆаᒤཊ⟏ࡦൠ४DŽҏҾᡁഭⲴ⦹㊣ѫӗ४ˈঐޘഭᙫ䶒〟Ⲵ 18.4%ˈঐ ޘഭᙫӗⲴ 13.4%DŽ㾯ই⦹㊣४ѫ㾱वਜ਼˖㾯ই᱕⦹㊣㊫ර४ˈ㾯ই勌伏⭌⦹㊣ǃ 勌伏㌟⦹㊣㊫ර४ˈ⠶㻲⦹㊣㊫ර४ㅹDŽ ইᯩ⦹㊣४˖䈕४䶒〟ᒯབྷˈवᤜᒯьǃ⎧ইǃ⎉⊏ǃ⊏㣿઼ᆹᗭই䜘ǃᒯ 㾯ь䜘ǃ⒆ই઼⒆ेⴱDŽ䈕४ѫ㾱Ἵ≤にˈ⦹㊣䶒〟䖳ሿˈਚঐޘഭ䶒〟 Ⲵ 3.2%ˈӗ䟿ਚঐޘഭᙫӗⲴ 2.2%DŽ䈕४սҾ✝ᑖ઼ӊ✝ᑖൠ४ˈᰐ䵌ᵏ 220~360 ཙˈ䱽䴘䟿 1000~1800 mmˈ䘲ਸ⦹㊣⭏䮯DŽҾаᒤй⟏ᡆаᒤഋ⟏ࡦൠ४DŽ ইᯩ⦹㊣४ѫ㾱वਜ਼˖✝ᑖӊ✝ᑖ⦹㊣㊫ර४ǃьই᱕⦹㊣㊫ර४ˈԕ৺ьই勌 伏⭌⦹㊣ǃ勌伏㌟⦹㊣㊫ර४ㅹDŽ 㾯े⦹㊣४˖䈕४Ѫᒢᰡൠ४ˈ䱽䴘䟿վҾ 200 mmˈवᤜᯠ⮶ǃ⋣㾯⭈㚳 䎠ᓺǃᆱ༿⋣྇ൠ४DŽ䘉аൠ४Ⲵ⢙ѫ㾱䶐♼ⒹDŽᰐ䵌ᵏ 130~180 ཙDŽ⦹㊣ 䶒〟ঐޘഭ䶒〟Ⲵ 3%DŽҾаᒤа⟏ࡦൠ४DŽ㾯े⦹㊣४ѫ㾱वਜ਼˖㾯 े᱕⦹㊣㊫ර४ˈԕ৺⠶㻲⦹㊣㊫ර४ㅹDŽ
മ5. ѝഭ⦹㊣Ἵ४ࡂമ
30 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 1.3.2 ⭕䮵ਇ㛨ᡶ㾷≸Ⲻ⭕ᘷ⧥ູᶗԬθऻᤢ㠠❬ᶗԬૂṳฯᶗԬⲺ ᭯ሯެ൦⨼࠼ᐹ॰ตૂ㤹പᖧଃⲺਥ㜳ᙝ 䘋ᆀ㨼ਁ઼ࠪ׳㊣⭏䮯ਁ㛢⨶ᜣⲴ⭏ᘱᶑԦवᤜݵ䏣Ⲵ൏༔ਜ਼≤䟿ǃ㜭⦹ ൏Ⲵᴰ֣൏༔ᓖ઼ݵ䏣Ⲵ൏༔ޫ࠶DŽ⦹㊣⭏ӗⲴᴰ֣ᰦᵏᱟᴰ✝ᴸԭⲴㅹ㓯 ൘ 21~27 C ѻ䰤ˈᰐ䵌ᵏ൘ 120~180 ཙѻ䰤DŽ15 cm Ⲵ༿ᆓ䱽䴘䟿㓖Ѫᰐ♼Ⓓᶑ Ԧл⦹㊣⭏ӗⲴ䱽䴘л䲀ˈ⦹㊣⭏䮯ᵏ䰤Ⲵ䱽䴘䟿㾱≲㲭ᰐк䲀ˈն䗷ཊⲴ䱽䴘 Պሬ㠤߿ӗDŽ ᓖᱟ⦹㊣⭏䮯Ⲵањޣ䭞ഐ㍐DŽᓖ䱽ࡠ 0°C ԕлՊᖡ૽ᆀⲴ㨼ਁˈഐ ↔վᱟᆀ⭏ӗᵏ䰤Ⲵѫ㾱仾䲙DŽ儈Ҿ 45°C ⲴᓖҏՊሩᆀⲴ⭏⍫࣋ӗ⭏ н㢟ᖡ૽˄Shaw, 1988˅DŽ൘⦹㊣䗮ࡠ⭏⇆⭏䮯䱦⇥˄ᣭょ㠣ᔰ㣡ᵏ˅ਾˈᤱ㔝 Ⲵ儈Պ߿ቁ㣡㊹Ⲵ⍫઼࣋ӗ䟿DŽ≤࠶൘⦹㊣Ⲵ⭏䮯ᆓ㢲ᱟ䲀ࡦᙗഐ㍐ˈቔަ൘ ⦹㊣Ⲵᔰ㣡ᵏ઼♼⍶ᵏˈ≤࠶н䏣ਟԕሬ㠤ᦸ㊹ഠ䳮ᡆ㜊㛾䍕㛢˄Claassen and Shaw, 1970; Boyer and Westgate, 2004; Campos et al., 2006˅DŽ ഐ↔ˈ䙊䗷䈳ᮤṭษ᧚ᯭˈྲӪᐕ♼Ⓓㅹˈਟԕᢙབྷ⦹㊣ⲴἽ४ฏ઼㤳തDŽ 1.3.3 ᱥੜѰ⭕ᘷ⧥ູѣⲺ㓺ᡆ䜞࠼ 㓿䗷䮯ᵏⲴӪᐕṭษ઼傟ॆˈ⦹㊣ᐢнᱟ㠚❦⭏ᘱ⧟ຳѝⲴ㓴ᡀ䜘࠶ˈ 㘼ᱟߌъ⭏ᘱ㌫㔏ѝањ䟽㾱㓴ᡀ䜘࠶DŽ↓ྲ൘ 1.1.7 㢲䟼䇘䇪Ⲵ䛓ṧˈ⦹㊣ᰙ ൘ 16 ц㓚ቡᶕࡠҶѝഭˈᱟᰙᵏⲴ⅗⍢Րᮉ༛ᑖᶕⲴDŽⴞࡽˈ⦹㊣൘ѝഭⲴཊ ᮠⴱԭ䜭ᴹἽ˄1.3.1 㢲˅DŽྲ൘ 1.2.8 ѝᡰᤷࠪⲴˈ⦹㊣н㜭൘䶎Ӫᐕṭษ ᶑԦл⭏ᆈDŽ 1.3.4 ф⭕ᘷ㌱㔕ѣެᆹὃ⢟Ⲻ⭕ᘷީ㌱θऻᤢ⭕ᘷ⧥ູⲺ᭯ሯ䘏 ᓭڛ㊱δӑεީ㌱ⲺᖧଃԛᱥੜՐഖ↚㙂ӝ⭕ᡌ໔ࣖሯӰ〃 ૂ⭕ᘷ⧥ູⲺуᖧଃ ྲкᡰ䘠ˈḀӋ໘㾯କ⦹㴰哽⢙ਟԕ਼⦹㊣ᵲӔӗ⭏ਟ㛢ਾԓˈնᱟ໘ 㾯କ⦹㴰哽ਚ⭏䮯൘໘㾯କ઼ডൠ傜˄Sánchez-González and Ruiz-Corral, 1997˅DŽ᪙ᬖⲴḀӋ⢙ਟԕ਼⦹㊣ᵲӔˈնᶱѪн᱃DŽᒦфᵲӔާᴹ 儈ᓖⲴн㛢ᙗˈнާᴹ䚇Րᆖっᇊᙗ˄Mangelsdorf, 1974˅DŽ↔ཆˈ⦹㊣⋑ᴹ 㻝⧠ᴹⲴ⭏ᘱ㌫㔏DŽഐ↔ˈ⭏ᘱ⧟ຳⲴץн㜭ᴹ᭸ൠˈੁٮᵲ㥹⢩ᙗ઼ᵲ㥹ॆ ⢙㻝ᙗᵲ㥹ᡆ䙐ᡀ〰ᴹץ᭩ਈሶнਟ㜭ሩ⦹㊣ӗ⭏䗷ཊⲴ⭏ᘱ᭸ᓄˈྲ╄ॆѪ ☂Ҿ⚝㔍ㅹDŽ ⦹㊣ᱟаᔲӔ⢙DŽྲ൘ 1.3.1 ѝᨀࡠⲴˈѝഭњൠ४䜭ᴹ⦹㊣ⲴἽDŽ 亴䇑⦹㊣઼ަԆ⢙ᡆἽ⢙Ⲵ⭏ᘱᆖޣ㌫нՊ⭡Ҿ⭏ᘱ⧟ຳⲴਈॆ㘼ਁ⭏᭩ਈDŽ 1.3.5 ф⭕ᘷ㌱㔕ѣެᆹ⭕⢟δࣞ⢟ૂᗤ⭕⢟εⲺ⭕ᘷީ㌱θऻᤢ⭕ ᘷ⧥ູⲺ᭯ሯ䘏〃δӑεީ㌱ⲺᖧଃԛᱥੜՐഖ↚㙂ӝ⭕ ᓭᡌ⭕ᘷ⧥ູⲺуᖧଃڛ㊱ᡌ໔ࣖሯӰ ൘⭏ᘱ㌫㔏ѝˈ⦹㊣਼൏༔ཆⲴަԆ⭏⢙ᴹаᇊⲴ⭏ᘱᆖޣ㌫DŽ⦹㊣ṩ 31 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ㌫⭡Ҿަ਼䈨ཊᗞ⭏⢙㗔փˈྲ㓶㧼ǃⵏ㧼ǃ᭮㓯㧼˄Vega-Segovia and Ferrera-Cerrato, 1996˅ǃ⭏ࣘ⢙઼㷘㊫ㅹᴹаᇊⲴޣ㌫ˈᡰԕ䘈㜭䎧ࡠ൏༔ ㌫˗⦹㊣਼ᰦҏᴹаӋᑨ㿱Ⲵ⯵ޣᤱ⭏ᘱ؍䈳㢲Ⲵ⭘DŽаӋᴹ⳺ᰶ㲛਼⦹㊣ 㲛ᇣDŽ䬸⯵ǃ唁㊹⯵ǃਦᯁ⯵઼㤾㞀⯵ㅹ䜭ᱟ⦹㊣кⲴᑨ㿱⯵ᇣˈަਁ⭏ᛵߥ ḃ⦹㊣ˈ㘼ᆳԜӗץ㓿⍾ᆖѹഐഭᇦ㘼ᔲDŽྲᴢ䴹㧼઼䮠࠰㧼ㅹⵏ㧼ҏՊ઼ ץ⭏Ⲵ䴹㧼∂㍐Պᖡ૽Ӫ㊫઼⢢⮌Ⲵ伏⭘DŽ㲛ᇣ઼ᒢᰡㅹ䘶ຳਟԕ࣐ⵏ㧼Ⲵ ḃ˄Dowd, 2001˅DŽ ⦹㊣Ⲵѫ㾱ᇣ㲛ᶕ㠚Ҿ匎㗵ⴞ઼䷈㗵ⴞᰶ㲛DŽ匎㗵ⴞⲴᒬ㲛ྲ⦹㊣㷏ਟԕ ਆ伏⦹㊣Ⲵਦ⡷ǃ㤾઼ょ䜘DŽ䷈㗵ⴞⲴᒬ㲛ྲ⦹㊣࠷ṩਦ⭢ਟԕਆ伏ṩ䜘ǃ㤾ǃ 㣡э઼㣡㊹ˈᡀ㲛ਟԕਆ伏ਦ䜘˄Dicke and Guthrie, 1988˅DŽ ਼⦹㊣а䎧ᆈ൘Ⲵ⭏⢙о⦹㊣Ⲵ⭏ᘱᆖޣ㌫ˈަԫօⲴਈॆˈ亴䇑ሶнՊ ᓧᡆ⧟ຳӗ⭏ԫօⲴ䍏䶒ᖡ૽DŽڕሩӪփ 1.3.6 ሯ⭕ᘷ⧥ູⲺᖧଃެ▒൞ধ䲟ぁᓜ ⦹㊣ᱟṭษ⢙ˈ൘ѝഭާᴹ䮯ᵏⲴṭษশਢˈሩ⭏ᘱ⧟ຳнՊ䙐ᡀ仾䲙DŽ 1.3.7 ⎿ࡦള䶔䙐ᑮ〃ὃⲺὃ⢟⢟〃ᰬθᓊᨅ䘦䈛ὃ⢟Ⲻ㠠❬⭕ ູૂᴿީެཟ❬ᦋ伕㘻Ƚᇺ⭕⢟Ƚㄔҿ⢟ૂާ⭕⢟Ⲻ䍺ᯏ ⦹㊣ާᴹ䮯ᵏⲴṭษশਢˈᱟѝഭഋབྷ⢙ѻаˈᱟ䟽㾱Ⲵ㋞伏઼侢ᯉ ⢙ˈ൘ѝഭᲞ䙽ἽDŽഐ↔нҾ䶎䙊ᑨἽⲴἽ⢙⢙DŽ 1.4 ։ὃ⢟Ⲻ䚍Ֆᔸ 1.4.1 䚍Ֆどᇐᙝ ⦹㊣ᱟ儈ㅹἽ⢙ѝᓄ⭘䖳ᒯⲴ䚇Րᆖ⹄ウ઼ᓄ⭘ᶀᯉDŽ⦹㊣ᱟᴰᰙ൘ᇎ傼 ᇔѝᔰ䚇Ր⹄ウⲴ⢙ѻаDŽᰙᵏӪԜሩ⦹㊣㓶㜎Ⲵᴹэ࠶㻲ǃ߿ᮠ࠶㻲ǃ ḃ㢢փ࠶о䘎䬱઼䖜ᓗᆀ᭸᷌ㅹ䚇Ր⧠䊑䜭䘋㹼Ҷᒯ⌋Ⲵ⹄ウDŽ⭡Ҿ⦹㊣൘ 㖾ഭ઼ц⭼㓿⍾ѝᢞ╄⵰䟽㾱Ⲵ䀂㢢ˈᡰԕ⦹㊣Ⲵ䚇Րᆖ⹄ウ㠣Ӻӽਇࡠ䟽㿶DŽ བྷ䟿Ⲵ䚇Րᆖ⹄ウ㔃᷌㺘᰾ˈ⦹㊣Ⲵᙗ⣦䙊䗷㠚Ӕ઼എӔਟԕっᇊ䚇ՐDŽ ⦹㊣䘋ॆᡀѪ㠚⭡ᦸ㊹⢙⢙ਾˈⴤࡠҼॱц㓚ˈ⦹㊣ṭษ૱ᡀѪ ӺཙѹкⲴᔲ㣡ᦸ㊹⢙DŽକՖᐳਁ⧠ᯠབྷ䱶ѻࡽˈᖃൠⲴ൏㪇Ӫ࡙⭘ㆰঅ Ⲵ㗔փ䘹ᤙˈษ㛢ҶаӋ૱DŽ൘ӺཙⴻᶕˈԆԜⲴ䘹㛢ᯩ⌅ᶱѪㆰঅˈն䘉 Ӌ䘹ᤙᯩ⌅㜭ॱ࠶ᴹ᭸ൠษ㛢૱ᡆ૱㌫ˈԕ┑䏣ԆԜሩ伏⢙ǃ⟳ᯉǃ侢ᯉ઼ ᮷ॆⲴ䴰≲DŽ䲿⵰㾯ॺ⨳᮷ॆⲴਁኅˈਁ⧠Ҷ૱䰤ᵲӔ㛢ᯩ⌅ˈ⭡↔ᔰ Ҷ࡙⭘Ἵ⢙Ⲵ䚇Րਈᔲᶕษ㛢⢩ᇊⲴ૱DŽ ॱҍц㓚Ҽॱᒤԓˈ、ᆖᇦྐᇊҶ⦹㊣ᵲӔ㛢ส⨶䇪DŽӪԜ䙊䗷䘋㹼 ⦹㊣૱䚇Ր㓴ᡀⲴส⹄ウˈᶕ⺞ᇊḀњ⢩ᇊ⦹㊣૱Ⲵ㠚㣡ᦸ㊹᭸ᓄ ˄Shull, 1909˅DŽᖃሩ⦹㊣অṚ䘎㔝䘋㹼 7 㠣 10 ԓⲴ㠚㣡ᦸ㊹ˈਟԕษ㛢ࠪ㓟 ㌫˄ণ㠚Ӕ㌫˅ˈ䘉њ㓟㌫㗔փѝⲴ⇿њἽṚ䜭ᴹ਼Ⲵ䚇Ր⢩ᙗDŽᆏᗧቄ䚇 Րᆖ⨶䇪ሩ൘㠚Ӕ䗷〻ѝਁ⭏Ⲵ䚇Րਈॆ䘋㹼Ҷ↓⺞䀓䟺˖䙊䗷㠚Ӕˈ֯⇿њ 32 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 สഐս⛩кⲴᵲਸㅹսสഐ䙀↕ਁ⭏㓟ਸˈᖒᡀ㓟㌫DŽ൘㓟㌫䟼ㅹսสഐⲴ㓟 ਸˈ֯ἽṚⲴ⍫઼࣋⭏ӗ࣋Პ䙽л䱽DŽ ᓭᡌ⭕ᘷ⧥ູӝ⭕уᖧଃڛ㊱ᱥੜᴿਇ⭕䚍Ֆᔸ㙂ሯӰ 1.4.2 Ⲻ䍺ᯏ ൘⦹㊣㛢䗷〻ѝˈ㛢ᇦ࡙⭘䈡ਈǃᵲӔㅹ⇥ࡋ䙐ᯠⲴ䚇Րਈᔲˈ⭘ ᶕ䘹㛢ᯠ૱DŽ⦹㊣൘㠚❦ᶑԦлҏՊӗ⭏аӋ䚇Րਈᔲˈ㛢ᇦҏ㓿ᑨᴹⴞ ⲴⲴ䘹ᤙǃ࡙⭘䘉Ӌ㠚❦ਈᔲ䘋㹼૱᭩㢟DŽⴞࡽᵚ㿱䘉Ӌ䚇ՐਈᔲሩӪ㊫ᡆ ⧟ຳӗн࡙ᖡ૽Ⲵ䍴ᯉDŽ 1.4.3 ൞㠠❬ᶗԬсфެᆹὃ⢟〃ኔ䘑㺂䚍Ֆ⢟䍞ӚᦘⲺਥ㜳ᙝ Ἵ⢙ᴹਟ㜭ਁ⭏สഐ┲〫ˈᒦ䙊䗷↓ᑨᴹᙗՐᇎ⧠สഐ⑀⑇ˈնᗵ享ާ ᆖ⢩ᙗᗵ享ᴹаᇊ䟽ਐˈণى⢙˅аᇊᶑԦ˖1˅єњӢᵜᗵ享ᴹᙗӢਸ˗2༷ 㣡ᵏ䙷˗3˅ᗵ享ᴹ䘲ᖃⲴ㣡㊹䖭փˈᆳ㜭൘єњӢᵜѻ䰤Ր䙂㣡㊹DŽ ⦹㊣઼аᒤ⭏໘㾯କ⦹㴰哽ާᴹ䚇ՐӢਸᙗDŽ൘໘㾯କ઼ডൠ傜ˈ䐍 䖳䘁ᰦˈ䙊䗷仾ჂՐ㊹ਟԕ㠚⭡ᵲӔ˄Wilkes, 1967; Sanchez et al.,1998˅DŽն ൘ѝഭ⋑ᴹ໘㾯କ⦹㴰哽DŽ ൘䟾⭏ᶑԦлˈ⦹㊣਼᪙ᬖ⢙ᵲӔᱟᶱѪഠ䳮Ⲵ˄Wilkes, 1972˅DŽ ഐѪ᪙ᬖἽ⢙ሩ⭏ຳⲴ㾱≲㊫լҾ໘㾯କ⦹㴰哽ˈᡰԕ൘ѝഭ⋑ᴹ᪙ᬖ Ἵ⢙DŽ ൞㠠❬ᶗԬсфެᆹ⭕⢟δׁྸᗤ⭕⢟ε䘑㺂䚍Ֆ⢟䍞ӚᦘⲺ 1.4.4 ਥ㜳ᙝ ᵚਁ⧠ԫօޣҾ䚇Ր⢙䍘⭡⦹㊣䙊䗷䰤ᴹᙗᵲӔੁަԆ⢙Ր䙂Ⲵᣕ䚃DŽ ⨶䇪к䇢ˈ䚇Ր⢙䍘䙊䗷≤ᒣՐ䙂ࡠަԆ⭏⢙ᱟਟ㜭Ⲵˈն൘㠚❦ᶑԦлˈӾ Ἵ⢙ੁࣘ⢙ᡆ㘵ᗞ⭏⢙Ⲵ≤ᒣสഐՐ䙂ˈ൘䈅傼кⴞࡽ䘈⋑ᴹᗇࡠ䇱ᇎ ˄Bertolla and Simonet, 1999˅DŽ 1.5 ։ὃ⢟Ⲻⴇ⎁ᯯ⌋ૂⴇⲺਥ㜳ᙝ ㊣Ⲵ⤜⢩䮯ǃ⣦ᘱ઼⭏䮯Ґᙗ֯ަ᱃Ҿ䇶઼࡛ⴁ⍻DŽ࡙⭘ḀӋ䘹ᤙᙗ⦹ 䲔㥹ࡲ઼ᒯ䉡䲔㥹ࡲˈྲ㥹⭈㟖ǃ䱯⢩⍕ǃ҉㥹㜪ㅹˈਟԕᴹ᭸᧗ࡦߌ⭠㌫ 㔏ѝнᜣ㾱Ⲵ⦹㊣DŽ↔ཆˈ䘈ਟԕ࡙⭘ѝ㙅Ⲵᯩ⌅䱢⋫⦹㊣Ⲵ㠚⭏㤇DŽ 1.6 ։ὃ⢟Ⲻެᆹ䍺ᯏ ㊣ⲴަԆ䍴ᯉਟԕ⦹ޣѪᵜ⭣䈧ⴞⲴˈ⋑ᴹᴤཊⲴ䍴ᯉ㾱ᨀDŽնᴤཊᴹ Ⲵᮉᶀǃ、ᢰҖ㉽ǃ㖁ㄉㅹؑ䚃к㧧ᗇDŽޣӾ 1.7 ṯᦤр䘦䇺ԭθ৸➝ᵢࣔ⌋ㅢॷжᶗᴿީḽࡈ࠼։ὃ⢟Ⲻ ᆿޞㅿ㓝
33 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ሩ⧟ຳ˗ੁٮкᡰ䘠ˈ⦹㊣ሩӪփᡆࣘ⢙⋑ᴹ∂ᙗ˗ᒦфнާᴹᵲ㥹ॆྲ ӗ⭏䍏䶒᭸ᓄᡆ㘵╄ਈᡀᴹᇣ⭏⢙Ⲵਟ㜭ᙗᶱሿDŽഐ↔ˈṩᦞߌъ䖜สഐ⭏⢙ ᆹޘ㇑⨶࣎⌅ㅜॱаᶑࡇࠪⲴḷ߶ˈਇփἽ⢙⦹㊣ᓄᆹޘㅹ㓗 I 㓗DŽ
34 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᙝ䇺ԭޞะഖᬃ֒Ⲻᆿ .2 侦ᙝ⣬ૂ⢯ᙝⲺਏ䘦ؤᡌޛ䖢ะഖὃ⢟ѣᕋ 2.1 ߌ䗮㊫䲔㥹ࡲѝⲴᴹ᭸ᡀ࠶㥹⭈㟖ᱟ 5-✟䞷щ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦 ˄EPSPS˅ⲴᣁࡦࡲDŽ㣣俉᯿≘ส䞨⭏⢙ਸᡀⲴ㧭㥹䞨䙄ᖴᲞ䙽ᆈ൘ҾἽ⢙ǃⵏ 㧼઼㓶㧼ѝˈ㘼 EPSPS ↓ᱟ㧭㥹䞨䙄ᖴѝⲴањޣ䭞䞦DŽߌ䗮㊫䲔㥹ࡲ࡙⭘䘉 а䙄ᖴˈԕ㥹⭈㟖䱫ᯝἽ⢙փ㣣俉᯿≘ส䞨Ⲵਸᡀˈ֯Ἵ⢙ഐ㣣俉᯿≘ส䞨㕪 ཡ㘼↫ӑDŽն൘ପңࣘ⢙ǃ呏㊫ᡆ≤⭏⭏⢙ѝˈнᆈ൘㧭㥹䞨䙄ᖴˈഐ↔㥹⭈㟖 ӵ⢩ᔲᙗൠሩἽ⢙ᴹ᭸DŽ MON 87427 Ⲵᨂޕ DNA ᒿࡇѝवਜ਼ᴹањᆼᮤⲴ cp4 epsp สഐ㺘䗮ⴂˈਟ ԕ㺘䗮 CP4 EPSPS 㳻ⲭDŽMON 87427 Ⲵ CP4 EPSPS 㳻ⲭབྷሿ㓖Ѫ 47.6 kDˈਜ਼ ᴹ 455 њ≘ส䞨˄മ 12˅ˈоἽ⢙ⓀⲴ EPSPS 㳻ⲭ࣏㜭਼ˈնо㥹⭈㟖ⲴӢ ਸᙗվˈഐ㘼֯ᗇ MON 87427 ਟԕ㙀ਇ㥹⭈㟖㊫䲔㥹ࡲDŽ MON 87427 䟷⭘⢩↺ࣘᆀ-ਜ਼ᆀ㓴ਸ˄e35S-hsp70˅ˈণ໎ᕪර 35S ࣘ ᆀ઼⦹㊣✝◰㳻ⲭ Hsp70 㕆⸱ᒿࡇⲴㅜањਜ਼ᆀDŽ䈕ࣘᆀ-ਜ਼ᆀ㓴ਸਟԕ ⢩ᔲᙗൠ൘㩕ޫಘᇈ˄ਦ⡷ǃ〨⿶઼ṩ˅઼䳼ᙗ⭏⇆ಘᇈ˄ਟԕ䘋а↕ਁ㛢ᡀ ᆀᡆ㊭㋂઼㣡эⲴ㓴㓷˅ѝ㺘䗮 CP4 EPSPS 㳻ⲭˈ֯䘉Ӌ㓴㓷઼ಘᇈਟԕᣇ㥹 ⭈㟖DŽն CP4 EPSPS 㳻ⲭ൘єњޣ䭞Ⲵ䳴ᙗಘᇈѝн㺘䗮ᡆ㺘䗮䟿ᴹ䲀˖㣡㊹ ሿᆒᆀ˄ਟԕ䘋а↕ਁ㛢ᡀ㣡㊹㋂˅઼∑㔂ቲ㓶㜎˄Ѫ㣡㊹㋂ਁ㛢ᨀ㩕ޫ˅DŽ ഐ↔ˈMON 87427 Ⲵ䳴ᙗಘᇈнᣇ㥹⭈㟖DŽ ⭘ਜ਼ᴹ MON 87427 䖜ॆփⲴ⦹㊣㠚Ӕ㌫⇽ᵜˈ⭘ަᆳᣇ㥹⭈㟖Ⲵ⦹㊣㠚 Ӕ㌫⡦ᵜˈ൘⦹㊣ V8~V13 ⭏䮯䱦⇥˄䳴ょⲴ⭏䮯ਁ㛢Ҿ㩕ޫ⭏䮯Ⲵ V9 䱦 ⇥˅௧ᯭє⅑㥹⭈㟖䲔㥹ࡲˈਟԕᣁࡦ⇽ᵜⲴ䳴ᙗಘᇈਁ㛢ˈӪᐕࡋ䙐䳴ᙗн㛢DŽ նਜ਼ᴹަᆳᣇ㥹⭈㟖䖜ॆփⲴ⡦ᵜⲴ䳴ᙗಘᇈਁ㛢ࡉнਇᖡ૽DŽ⭡↔ᖒᡀн⭘Ӫ ᐕ৫䳴ⲴᵲӔࡦ㌫㔏DŽ 2.2 ᇔ䱻ᨈޛᡌࡖ䲚ᓅࡍⲺԛс䍺ᯏ 2.2.1 ᨈޛᓅࡍⲺཝቅૂ㔉ᶺθ⺤ᇐެ⢯ᙝⲺ࠼᷆ᯯ⌋ MON 87427 ᱟ䙊䗷ߌᵶ㧼ӻሬ䖜ॆˈሶ PV-ZMAP1043 䍘㋂˄മ 6˅ѝⲴ T-DNA ሬޕࡠ LH198 × HiII ਇփ૱㌫ѝ㧧ᗇⲴDŽᶕ㠚Ҿ PV-ZMAP1043 䖜ॆ䍘 ㋂Ⲵ T-DNA वਜ਼ᴹањ cp4 epsps สഐ㺘䗮ⴂˈ䈕สഐ㺘䗮ⴂ⭡ e35S ࣘᆀǃ hsp70 Ⲵਜ਼ᆀǃCTP2 ਦ㔯փ䖜〫㛭ᒿࡇǃcp4 epsps 㕆⸱ᒿࡇ઼ nos 㓸→ᆀᶴ ᡀDŽ Southern ᵲӔ㔃᷌㺘᰾ˈMON 87427 ӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ⦹㊣สഐ㓴Ⲵ অњสഐᓗսкˈᒦф䲔 T-DNA ཆˈ⋑ᴹỰ⍻ࡠ MON 87427 ѝᆈ൘ަᆳᶕ㠚 Ҿ PV-ZMAP1043 䖜ॆ䍘㋂Ⲵᒿࡇ˄Southern ᵲӔ࠶᷀㔃᷌㿱ԕл 2.2.5 䜘࠶˅DŽ ࡙⭘ PCR ᯩ⌅ᢙ໎Ҷ 3 њӂ䟽ਐⲴ DNA ⡷⇥˄മ 7ˈӗ⢙ Aǃӗ⢙ B ઼ 㘬สഐ㓴ᒿࡇDŽPCR ᢙ໎ᡰ⭘חᒿࡇ৺ަޕ䮯ᨂޘӗ⢙ C˅ˈ䘉 3 њ⡷⇥⏥ⴆҶ ᕅ⢙઼ᶑԦ㿱㺘 5DŽሶ䘉 3 њᢙ໎ӗ⢙䘋㹼ᒿࡇ࠶᷀ᒦ᧕ˈণਟ⺞ᇊޘ䮯ᨂޕ
35 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᒿࡇབྷޕ㘬ᒿࡇ˄മ 8˅DŽṩᦞᒿࡇ࠶᷀㔃᷌ˈMON 87427 Ⲵᨂחᒿࡇ৺สഐ㓴 ሿѪ 3746 bpˈަ ѝ T-DNA ᨂޕѪ 3681 bpDŽਖཆ൘ T-DNA Ⲵ 5'ㄟ઼ 3'ㄟ࠶࡛ᴹ 41 bp ઼ 24 bp Ⲵᨂޕˈ䘉ᱟ൘ T-DNA ᨂޕ䗷〻ѝ㠚㹼ᨂޕⲴᒿࡇDŽ䘉⧠䊑൘ 䗷〻ѝᑨ㿱ˈਟ㜭ᱟ⭡Ҿৼ䬮ᯝ㻲؞༽ᵪࡦᕅ䎧˄Salomon andޕཆⓀ DNA ᨂ Puchta, 1998˅DŽ ↔ཆˈᒿࡇ࠶᷀ҏ䇱ᇎ T-DNA cp4 epsps สഐ㺘䗮ⴂᱟᆼᮤⲴˈަѝ䚇 ՐݳԦⲴᧂࡇᯩᔿоަ൘ PV-ZMAP1043 䖜ॆ䍘㋂кⲴᧂࡇᯩᔿа㠤DŽ ᒿࡇѝޕ㘬ᒿࡇԕ৺㔃ᶴ㿱മ 13 ઼മ 8˗ᨂחᒿࡇ઼สഐ㓴ޕMON 87427 Ⲵᨂ Ⲵ䈳᧗ݳԦ᧿䘠㿱㺘 6DŽ ˅䶒Ⲵ⭏⢙ؑᆖ࠶᷀ˈԕ⺞ᇊ˖1ޘս⛩䱴䘁४ฏ䘋㹼ޕሩ MON 87427 ᨂ MON 87427 ⦹㊣ѝᨂޕ㔃⛩४䱴䘁Ⲵԫօਟ㜭ཊ㛭оᴤᯠⲴ∂㍐㳻ⲭ઼ᣇ㩕ޫ ഐᆀǃ㠤㳻ⲭ઼࣏㜭⍫ᙗ㳻ⲭᮠᦞᓃѝⲴᒿࡇѻ䰤ᱟᆈ൘լᙗ˗2˅MON 87427 ѝᨂޕⲴ T-DNA 㕆⸱ᒿࡇо∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃ㠤㳻ⲭ઼࣏㜭⍫ ᙗ㳻ⲭᮠᦞᓃѝⲴᒿࡇѻ䰤ᱟᆈ൘լᙗ˗3˅MON 87427 ᱟ⭡ҾཆⓀ DNA Ⲵᨂޕ㘼ൿҶᴹⲴ⦹㊣ⓀสഐⲴ㺘䗮ˈᡆ㘵ӗ⭏ҶᯠⲴᔰ᭮䈫⸱Ṷ˄ORF˅DŽ ⭏⢙ؑᆖ࠶᷀㔃᷌䈖㿱䲿ᵜ⭣䈧䍴ᯉᨀӔⲴᢰᵟᣕ 7DŽ⭏⢙ؑᆖ࠶᷀֯⭘ Ⲵᴰᯠ؞䇒઼ᴤᯠⲴᮠᦞᓃवᤜ˖䗷ǃ䞷ⓦ㳻ⲭ઼哖䉧㳻ⲭᒿࡇᮠᦞᓃ ˄AD_2016˅ǃ∂㍐ᮠᦞᓃ˄TOX_2016˅ǃGenBank 㳻ⲭ䍘ᮠᦞᓃ˄PRT_2016˅ǃ GenBank 㺘䗮ᒿࡇḷㆮᮠᦞᓃ˄EST˅ǃ䶎߇։Ṩ㤧䞨ᮠᦞᓃ˄NT_2016˅ԕ৺ 䶎߇։≘ส䞨ᮠᦞᓃ˄NR_2016˅DŽ ᇊᒿࡇԕ৺Ӿٷ㔃⛩४㘫䈁㘼ᗇⲴ 14 ޕ㘬ᒿࡇ-ᨂח’⭘Ӿ 5’ᡆ 3֯ T-DNA 㘫䈁Ⲵ 6 䈫⸱ṶѪỰ㍒ᒿࡇˈ൘ AD_2016ǃTOX_2016 ઼ PRT_2016 ᮠᦞᓃѝ࠶࡛䘋㹼 FASTA ઼ޛ≘ส䞨━ࣘデਓᩌ㍒DŽᩌ㍒㔃᷌㺘᰾ˈ㲭❦⨶䇪 ˈᇊᒿࡇ઼Ӿ T-DNA 㘫䈁Ⲵ 6 䈫⸱Ṷ൘Ἵ⢙ѝᆈ൘Ⲵਟ㜭ᙗᶱվٷк䘉 14 ᓧⲴ∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃڕ⢙ণ֯ᆈ൘ˈ䘉Ӌᒿࡇоᐢ⸕ਟ㜭ᖡ૽Ӫ㊫ᡆࣘ 㠤㳻ⲭ઼࣏㜭⍫ᙗ㳻ⲭѻ䰤൷ᰐᱮ㪇ᒿࡇլᙗᡆ਼ⓀᙗDŽሶ MON 87427 ѝ 㘬ᒿࡇ൘ EST_2016 ઼ NT_2016 ᮠᦞᓃѝ䘋㹼 BLASTnחս⛩䱴䘁Ⲵสഐ㓴ޕᨂ ࠶᷀ˈ൘ NR_2016 ᮠᦞᓃѝ䘋㹼 BLASTx ࠶᷀ˈ൷ᵚਁ⧠ MON 87427 ᨂޕս 㘬สഐ㓴 DNA 䜘ᡆ䱴䘁ᆈ൘ਟ㜭㕆⸱ᯠ㳻ⲭӗ⢙Ⲵ ORFˈҏ⋑ᴹⓀᙗח⛩ Ⲵ ORF ഐѪ T-DNA Ⲵᨂޕ㘼㻛ൿDŽ
36 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 B-Left Border Region OR-ori V P-e35S
I-hsp70 Nsi I1719 Nco I1952 TS-CTP2 CS-rop T-DNA PV-ZMAP1043 Sph I2184 6 8946 bp
OR-ori-pBR322 CS-cp4 epsps
Nsi I3676 aadA T-nos Sph I3986 B-Right Border Region
᧒䪸 Ự⍻ⴞⲴ 䎧ս⛩˄bp˅ 㓸→ս⛩˄bp˅ ᧒䪸ޘ䮯˄~kb˅ 1 T-DNA 1 1200 1.2 2 T-DNA 1150 2150 1.0 3 T-DNA 2100 3550 1.5 4 T-DNA 3500 4192 0.7 5 䍘㋂僘ᷦ 4193 5942 1.8 6 䍘㋂僘ᷦ 5864 7368 1.5 7 䍘㋂僘ᷦ 7290 8946 1.7 മ6. PV-ZMAP1043䍘㋂മ˄⽪ᵲӔ᧒䪸1-7˅ кമѪُࣙҾߌᵶ㧼䖜ॆᯩ⌅ᗇࡠᣇ㥹⭈㟖䖜สഐ⦹㊣ MON 87427 ᰦᡰ˖⌘ ḷ᰾Ҷ䚇ՐݳԦ઼ח⭘㺘䗮䖭փ PV-ZMAP1043 Ⲵ⧟ᖒ䍘㋂മ䉡DŽ䍘㋂മཆ Southern ᵲӔ࠶᷀ᡰ⭘䲀ࡦᙗ࠷䞦ս⛩˄㋇փ˅ˈSouthern ᵲӔ࠶᷀ᡰ⭘᧒䪸˄᧒ DŽח䪸 1~7˅ሩս㖞ḷҾ⧟ᖒമ
37 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ
˅㘬สഐ㓴⡷⇥Ⲵ䟽ਐ PCR ࠶᷀˄CBIח४৺ޕമ 7. ⏥ⴆ MON 87427 ᨂ ᢙ໎⡷⇥Ⲵ亴ᵏབྷሿᱮ⽪൘ PCR ӗ⢙ࠍ㜦മлᯩⲴ MON 87427 ᨂޕᒿࡇ㓯 ᖒമкDŽ⇿⌣䚃кṧ䟿Ѫ 4~9 lPCRᢙ໎ӗ⢙DŽ⌣䚃䈤᰾ྲл˖
⌣䚃 ṧ૱᧿䘠 1 1 kb DNA ࠶ᆀ䟿ḷ䇠 2 MON 87427 3 ሩ➗ LH198 × HiII 4 ᰐ DNA ⁑ᶯሩ➗ 5 MON 87427 6 ሩ➗ LH198 × HiII 7 ᰐ DNA ⁑ᶯሩ➗ 8 MON 87427 9 ሩ➗ LH198 × HiII 10 ᰐ DNA ⁑ᶯሩ➗ 11 1 kb DNA Ladder
㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠
38 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ
˅㘬⦹㊣สഐ㓴ᒿࡇ˄CBIחᒿࡇ઼ޕ䮯ᨂޘ മ 8. MON 87427
39 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᒿࡇPCRᢙ໎࠶᷀˄CBIחᒿࡇ৺สഐ㓴ᯱޕ䮯ᨂޘ㺘5. MON 87427
ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ
40 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᒿࡇѝⲴ䚇Ր䈳᧗ݳԦ৺ަᆳᒿࡇ≷ᙫޕ㺘6. MON 87427ᨂ
˅⥞䚇ՐݳԦ བྷሿ˄ս㖞*˅ ࣏㜭᧿䘠˄৲㘳᮷ 1003 bp ս⛩ 5'ㄟ䛫Ⲵ⦹㊣สഐ㓴 DNAޕ㘬ᒿࡇ ᨂחㄟ'5 ˄1-1003˅ 252 bp ṩⱼߌᵶ㧼 T-DNA ᐖ䗩⭼ᒿࡇˈ৲о T-DNA T-DNA ᐖ䗩⭼ᒿࡇ ˄1004-1255˅ 䖜઼〫ᮤਸ˄Barker et al., 1983˅ 41 bp 䰤ᨂᒿࡇ DNA ݻ䲶઼䖭փᶴᔪ䴰㾱 ˄1256-1296˅ Kay et al., 1987 621 bp वਜ਼ᴹ䟽༽໎ᕪ४ฏ˄ ˅Ⲵ㣡 e35S ࣘᆀ ὠ㨌㣡ਦ⯵∂˄CaMV˅35S RNA Ⲵࣘᆀ ˄1297-1917˅ ˄Odell et al., 1985˅ 21 bp 䰤ᨂᒿࡇ DNA ݻ䲶઼ᶴᔪ䴰㾱 ˄1918-1938˅ 804 bp ⦹㊣✝◰㳻ⲭ 70 สഐⲴㅜањਜ਼ᆀ˄Brown hsp70 ਜ਼ᆀ ˄1939-2742˅ and Santino, 1997˅ 24 bp 䰤ᨂᒿࡇ DNA ݻ䲶઼䖭փᶴᔪ䴰㾱 ˄2743-2766˅ EPSPS 228 bp 㕆⸱ᤏই㣕 䞦ਦ㔯փ䖜〫㛭४ฏⲴส CTP2 ਦ㔯փ䖜〫㛭 ഐ ShkG Ⲵ䶦ḷᒿࡇ˄Herrmann, 1995; Klee et ˄2767-2994˅ al., 1987˅ˈᕅሬ CP4 EPSPS 䖜〫ࡠਦ㔯փ
1368 bp 㓿ᇶ⸱ᆀՈॆⲴṩⱼߌᵶ㧼 CP4 㧼Ṛ aroA ส cp4 epsps 㕆⸱ᒿࡇ ഐ㕆⸱ᒿࡇˈ㕆⸱ CP4 EPSPS 㳻ⲭ˄Barry et ˄2995-4362˅ al., 1992; Padgette et al., 1996˅ 6bp 䰤ᨂᒿࡇ DNA ݻ䲶઼䖭փᶴᔪ䴰㾱 ˄4363-4368˅ nos 3' 253 bp ṩⱼߌᵶ㧼㜝㜲⻡ਸ䞦สഐ˄ ˅ ㄟ䶎䖜ᖅ nos 㓸→ᆀ ४ˈ㓸→䖜ᖅᒦфᕅሬ mRNA Ⲵཊ㚊㞪㤧䞨 ˄4369-4621˅ ॆ˄Bevan et al., 1983˅ 27 bp 䰤ᨂᒿࡇ DNA ݻ䲶઼䖭փᶴᔪ䴰㾱 ˄4622-4648˅ T-DNA T-DNA 36 bp ṩⱼߌᵶ㧼 ਣ䗩⭼ᒿࡇˈ৲о T-DNA ਣ䗩⭼ᒿࡇ 䖜઼〫ᮤਸ˄Depicker et al., 1982; Zambryski ˄4649-4684˅ et al., 1982˅ 1092 bp ս⛩ 3'ㄟ䛫Ⲵ⦹㊣สഐ㓴 DNAޕ㘬ᒿࡇ ᨂחㄟ'3 ˄4685-5776˅
㘬ᒿࡇ˄㿱മ 8˅Ⲵᓄս㖞DŽחᒿࡇ઼ޕᤜਧḷࠪⲴս㖞ሩᓄҾ MON 87427 ᨂ *
41 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᕅ⢙ᔰਁࠪҶ MON 87427ޣ㘬ᒿࡇ˄മ 8˅ˈ䇮䇑חᒿࡇ઼สഐ㓴ޕṩᦞᨂ 䖜ॆփ⢩ᔲᙗⲴᇊᙗ઼ᇊ䟿Ự⍻ᯩ⌅˄䈖㿱ᵜ⭣䈧Җㅜ 171 亥Āㅜޝ䜘࠶ޣ䱴 Ԧ䍴ᯉĀ4. 䖜สഐᙗ⣦৺ӗ⢙ⲴỰ⍻઼䢤ᇊᢰᵟā˅DŽᇊᙗỰ⍻ᯩ⌅Ѫࠍ㜦⭥⌣ PCR ᯩ⌅ˈᇊ䟿Ự⍻ᯩ⌅Ѫᇎᰦᇊ䟿 PCR ᯩ⌅DŽᇊᙗ઼ᇊ䟿Ự⍻ᯩ⌅ᡰ⭘ᕅ⢙ǃ ᕅ⢙ս㖞ԕ৺ᢙ໎⡷⇥བྷሿྲл˖ ᇊᙗỰ⍻˄CBI˅˖
ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ
ᇊ䟿Ự⍻˄CBI˅˖
ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ
മ 9.䖜สഐ⦹㊣ MON 87427 ࠍ㜦 PCR ᇊᙗỰ⍻ᯩ⌅Ⲵ⢩ᔲᙗ傼䇱 ㇝ཤᤷ⽪ PCR ӗ⢙Ⲵ⡷⇥䱴䘁࠶ᆀ䟿ḷ䇠˗ަѝˈA മѪ֯⭘ MON 87427˖⌘ 䖜ॆփ⢩ᔲⲴᇊᙗỰ⍻ᕅ⢙䘋㹼 PCR Ⲵ㔃᷌˗B മѪ֯⭘ hmgAᇊᙗᕅ⢙䘋㹼 PCR Ⲵ㔃᷌DŽ⌣䚃ѝᡰ⭘ DNA ⁑ᶯ᧿䘠㿱л亥㺘ṬDŽ
ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ
42 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2.2.2 ࡖ䲚॰ตⲺཝቅૂࣕ㜳 㘬Ⲵ DNA ᒿࡇоཙ❦⦹㊣สഐ㓴਼ˈᒦחޕѪҶ⺞ᇊ MON 87427 ѝᨂ 䢤ᇊᑨ㿴ሩ➗ LH198 × HiII สഐ㓴ѝ MON 87427 Ⲵᨂޕս⛩ˈሩ MON 87427 ઼ᑨ㿴ሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA 䘋㹼 PCR ࠶᷀DŽṩᦞ MON 87427 ᨂޕ 㘬ᒿࡇкˈᕅח㘬ᒿࡇ䇮䇑аሩᕅ⢙ˈަѝᕅ⢙ A սҾ 5'ㄟחєㄟ䛫Ⲵ⇥⡷ 㘬ᒿࡇкˈሩᑨ㿴ਇփሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA 䘋㹼חB սҾ 3'ㄟ ⢙ PCR ᢙ໎࠶᷀˄മ 10˅DŽ❦ਾሩ PCR ᢙ໎ӗ⢙䘋㹼⍻ᒿˈᒦо MON 87427 ᨂޕ 㘬ᒿࡇ˄മ 8˅䘋㹼∄ሩˈਁ⧠ਇփ૱สഐ㓴ѝᴹ 140 bpחⲴ 5'ㄟ㠣 3'ㄟ⇥⡷ Ⲵᒿࡇ⋑ᴹࠪ⧠൘ MON 87427 Ⲵสഐ㓴 DNA ѝˈਟ㜭ᱟ൘ T-DNA ᮤਸᰦਁ⭏ Ҷࡐ䲔DŽⴞࡽሩҾཆⓀสഐᨂޕᰦᕅ䎧สഐ㓴ᒿࡇ䟽㓴Ⲵᵪࡦ䘈нॱ࠶ᾊˈᖸ ᴹਟ㜭ᱟ⭡Ҿ DNA ᮤਸᰦৼ䬮ᯝ㻲؞༽ᵪࡦᕅ䎧˄Salomon and Puchta, 1998˅DŽ 㻛ࡐ䲔Ⲵ 140 bp ᒿࡇྲл˖
ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ
43 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ
മ10. MON 87427ਇփᑨ㿴⦹㊣Ⲵᨂޕս⛩PCR࠶᷀˄CBI˅
44 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2.2.3 ⴤⲺะഖⲺṮ㤭䞮ᓅࡍૂ᧞ሲⲺ≞ะ䞮ᓅࡍ MON 87427 ⲴᨂޕᒿࡇབྷሿѪ 3746 bpˈ㘼 T-DNA ᨂޕᒿࡇབྷሿѪ 3681 bpDŽ 䲔 T-DNA ཆˈ൘T-DNAⲴ5'ㄟ઼ 3'ㄟ࠶࡛ᴹ 41 bp઼ 24 bp Ⲵᨂޕˈ䘉ᱟ൘ T-DNA ᨂޕ䗷〻ѝ㠚㹼ᨂޕⲴᒿࡇDŽT-DNA ѝⲴⴞⲴสഐ cp4 epsps ⲴབྷሿѪ 1368 bpDŽ cp4 epsps ⲴṨ㤧䞨ᒿࡇ㿱മ ˈᖃҾമ 8˄MON 87427 ޘ䮯ᨂޕᒿࡇ઼สഐ 㘬 DNA ᒿࡇ˅ᡰ⽪Ⲵ 2995~4362 bp Ⲵս㖞DŽח㓴 MON 87427 㺘䗮Ⲵ CP4 EPSPS 㳻ⲭབྷሿ㓖Ѫ 47.6 kDˈਜ਼ᴹ 455 њ≘ส䞨ˈ ṩᦞⴞⲴสഐṨ㤧䞨ᒿࡇ᧘ሬⲴ CP4 EPSPS 㳻ⲭⲴ≘ส䞨ᒿࡇ㿱മ 12DŽ
ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ
മ11. MON 87427ѝcp4 epspsสഐⲴṨ㤧䞨ᒿࡇ˄CBI˅ 1 MLHGASSRPA TARKSSGLSG TVRIPGDKSI SHRSFMFGGL ASGETRITGL 51 LEGEDVINTG KAMQAMGARI RKEGDTWIID GVGNGGLLAP EAPLDFGNAA 101 TGCRLTMGLV GVYDFDSTFI GDASLTKRPM GRVLNPLREM GVQVKSEDGD 151 RLPVTLRGPK TPTPITYRVP MASAQVKSAV LLAGLNTPGI TTVIEPIMTR 201 DHTEKMLQGF GANLTVETDA DGVRTIRLEG RGKLTGQVID VPGDPSSTAF 251 PLVAALLVPG SDVTILNVLM NPTRTGLILT LQEMGADIEV INPRLAGGED 301 VADLRVRSST LKGVTVPEDR APSMIDEYPI LAVAAAFAEG ATVMNGLEEL 351 RVKESDRLSA VANGLKLNGV DCDEGETSLV VRGRPDGKGL GNASGAAVAT 401 HLDHRIAMSF LVMGLVSENP VTVDDATMIA TSFPEFMDLM AGLGAKIELS 451 DTKAA മ12. ṩᦞMON 87427ѝcp4 epspsสഐⲴṨ㤧䞨ᒿࡇ᧘ሬⲴ CP4 EPSPS㳻ⲭⲴ≘ส䞨ᒿࡇ 2.2.4 ᨈޛᓅࡍ൞ὃ⢟㓼㜔ѣⲺᇐփδᱥੜ᮪ਾࡦḉ㢨։Ƚਬ㔵։Ƚ 㓵㋈։θᡌԛ䶔᮪ਾᖘᕅᆎ൞εެ⺤ᇐᯯ⌋ MON 87427 ѝ T-DNA ൘ਇփสഐ㓴Ѫঅᤧ䍍অս⛩ᨂޕˈަ൘ਾԓⲴสഐ 㓴ѝⲴ࠶ҏㅖਸ㓿ިᆏᗧቄ䚇Ր࠶∄˄࠶࡛㿱ㅜ 2.2.5 䜘࠶ᨂޕᒿࡇⲴᤧ䍍 ᮠ࠶઼᷀ㅜ 3.1 䜘࠶ᨂޕっᇊᙗ࠶᷀˅DŽ࡙⭘৽ੁ PCR ᶕᢙ໎ᨂޕս⛩ཆสഐ 㘬ᒿࡇDŽṩᦞᐢ⸕חᒿࡇⲴสഐ㓴ޕ㓴 DNAˈ❦ਾሩᢙ໎⡷⇥⍻ᒿˈԕ⺞ᇊᨂ Ⲵ⦹㊣สഐ㓴ᒿࡇⲴ⢙⨶䘎䬱㗔ˈ࡙⭘ GMAP ㇇⌅˄Wu and Watanabe, 2005˅ˈ 㘬ᒿࡇᇊս൘⦹㊣ㅜ 9 ਧḃ㢢փк䐍ѝᗳ⵰э㋂ 60חᒿࡇⲴޕሶ MON 87427 ᨂ ৈ᪙༴DŽഐ↔ᴰ㓸ਟԕ⺞ᇊ MON 87427 ѝᨂޕᒿࡇᮤਸࡠ⦹㊣ⲴṨสഐ㓴ѝˈ սҾㅜ 9 ਧḃ㢢փкDŽ
45 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2.2.5 ᨈޛᓅࡍⲺ䍓ᮦ ⭘䲀ࡦᙗ࠷䞦 Nco I ᡆ Nsi I ⎸ॆ MON 87427 สഐ㓴 DNAˈԕ㜭ཏ⏥ⴆ T-DNA ᒿࡇޘ䮯Ⲵӂ䟽ਐ᧒䪸˄᧒䪸 1~᧒䪸 4ˈമ 6 ઼മ 13˅䘋㹼 Southern ᵲӔ࠶᷀ˈӾ㘼⺞ᇊ MON 87427 ѝⲴᨂޕս⛩ᮠ઼ᨂޕᤧ䍍ᮠDŽ 㘬ᒿࡇⲴ㓯ᙗ⢙⨶മ䉡˄മ 13˅ˈ⭘䲀ࡦᙗ࠷䞦 Nco Iחᒿࡇ৺ަޕṩᦞᨂ 㘬ᒿࡇкᴹ 1 њ䞦חⲴ T-DNA 䜘ਚᴹ 1 њ䞦࠷ս⛩ˈᒦ൘䛫Ⲵ 3'ㄟޕ൘ᨂ ࠷ս⛩DŽྲ᷌ T-DNA ൘ MON 87427 ѝѪঅս⛩অᤧ䍍ᨂޕˈࡉ亴ᵏ⭘ Nco I 䞦 ⎸ॆ MON 87427 สഐ㓴 DNA ਾ㜭ཏӗ⭏ 2 њ䞦࠷⡷⇥ˈަѝањ⡷⇥བྷሿ亴 㘬ᒿࡇˈ㘼חᵏѪ~2.2 kbˈवਜ਼ CTP2ǃcp4 epspsǃnosǃਣ䗩⭼ᒿࡇ઼ 3'ㄟ䜘࠶ 㘬ᒿࡇǃᐖ䗩⭼ᒿࡇǃחਖཆањ䗩⭼⡷⇥亴ᵏı2.8 kbˈवਜ਼ᵚ⸕བྷሿⲴ 5'ㄟ e35S ઼ hsp70DŽ 㘬ᒿח䲀ࡦᙗ࠷䞦 Nsi I ൘ T-DNA 䜘ᴹ 2 њ䞦࠷ս⛩ˈ൘ަ䛫᧕Ⲵ 5'ㄟ ࡇкᴹ 1 њ䞦࠷ս⛩DŽྲ᷌ T-DNA ൘ MON 87427 ѝѪঅս⛩অᤧ䍍ᨂޕˈࡉ 亴ᵏ⭘ Nsi I ⎸ॆ MON 87427 สഐ㓴 DNA ਾ㜭ཏӗ⭏ 3 њ䞦࠷⡷⇥DŽަѝаᶑ ˗㘬ᒿࡇǃᐖ䗩⭼ᒿࡇǃe35S ઼䜘࠶ hsp70חkbˈ䈕ᶑᑖवᤜᐢ⸕བྷሿⲴ 5'ㄟ 1.7~ аᶑᑖ~2.0 kbˈवᤜ䜘࠶ hsp70ǃCTP2ǃcp4 epsps ઼䜘࠶ nos˗ਖཆаᶑᑖı1.3 kbˈ 㘬ᒿࡇDŽחवᤜ䜘࠶ nosǃਣ䗩⭼ᒿࡇ઼ᵚ⸕བྷሿⲴ 3'ㄟ MON 87427 ᱟԕ LH198 × HiII ᵲӔѪਇփ䖜ॆⲴˈഐ↔䟷⭘ LH198 × HiII Ѫ䱤ᙗሩ➗ˈ਼ᰦ⭘㓿 Sph I 䞦࠷Ⲵ PV-ZMAP1043 䍘㋂ DNA ᡆ ⺞᧒䪸Ѫ䱣ᙗሩ➗DŽ䱣ᙗሩ➗кṧ䟿Ѫ~0.1 ؽ઼~1.0 ؽสഐ㓴 DNA Ⲵ䟿ˈԕ ᇊ Southern ᵲӔⲴ⚥ᓖDŽ⭘᧒䪸 1~4˄മ 6 ઼മ 13˅䘋㹼 Southern ᵲӔਟ㜭 Պࠪ⧠ⲴᵲӔᑖර≷ᙫҾ㺘 7ˈᵲӔ㔃᷌㿱മ 14ǃമ 15 ઼മ 16DŽ ൘ MON 87427 ᨂޕᒿࡇѝнवਜ਼ PV-ZMAP1043 䍘㋂Ⲵԫօ僘ᷦᒿࡇDŽ਼ ṧ⭘ Nco I ᡆ Nsi I 䲀ࡦᙗ࠷䞦⎸ॆ MON 87427 สഐ㓴 DNAˈᒦ⭘᧒䪸 5~7 䘋㹼ᵲӔਟԕ⺞ᇊ MON 87427 ᱟਜ਼ᴹ僘ᷦᒿࡇDŽ᧒䪸 5~7 ਟԕ㾶ⴆ PV-ZMAP1043 䍘㋂Ⲵޘ䜘僘ᷦᒿࡇ˄മ 6 ઼മ 13˅DŽ⭘᧒䪸 5~7 䘋㹼 Southern ᵲӔਟ㜭Պࠪ⧠ⲴᵲӔᑖර≷ᙫҾ㺘 7DŽSouthern ᵲӔ㔃᷌㿱മ 17DŽ
46 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘7. MON 87427ⲴSouthernᵲӔѝ䲀ࡦᙗ࠷䞦ǃᵲӔ᧒䪸઼亴ᵏ⡷⇥≷ᙫ
Southern ᵲӔ㔃᷌ മ 14 മ 15 മ 16 മ 17 ⭘Ⲵ᧒䪸 ᧒䪸 1 ઼ 4 ᧒䪸 2 ᧒䪸 3 ᧒䪸 5ǃ6 ઼ 7֯ Ự⍻ⴞⲴ ᨂޕ/ᤧ䍍ᮠ ᨂޕ/ᤧ䍍ᮠ ᨂޕ/ᤧ䍍ᮠ 䍘㋂僘ᷦᒿࡇ
᧒䪸䶦ḷᒿࡇ ࠷䞦 亴ᵏ Southern ᵲӔؑਧ ~7.1 kb ~7.1 kb 䍘㋂ PV-ZMAP1043 Sph I ~7.1 kb ~7.1 kb ~1.8 kb ~1.8 kb ~1.8 kb ~1.2 kb ᧒䪸⁑ᶯ --* ~1.0 kb ~1.5 kb ~1.5 kb ~0.7 kb ~1.7 kb ı 2.8 kb ı 2.8 kb Nco I ~2.2 kb ᰐᵲӔᶑᑖ ~2.2 kb ~2.2 kb MON 87427 ~1.7 kb ~2.0 kb Nsi I ~2.0 kb ~2.0 kb ᰐᵲӔᶑᑖ ~1.7 kb ı 1.3 kb
*㺘⽪ᵚ⭘ᓄ䞦䘋㹼䞦࠷࠶᷀
47 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427
Nsi I 2533 Nsi I 4490 Nco I4931 Nsi I 795 Nco I2766 r1 r1
1 nos - e35S - T
hsp70 CTP2 5776 - - P I TS cp4 epsps - CS Left Border Region - B B-Right Border Region
Probe 1 Probe 2
Probe 3
Probe 4 ~ 2.2 kb Nco I
~ 1.7 kb ~ 2.0 kb Nsi I
㘬ᒿࡇ৺亴ᵏ䞦࠷⡷⇥བྷሿⲴ㓯ᙗ⽪മחᒿࡇǃสഐ㓴ޕമ13. MON 87427ᨂ ᒿࡇлᯩ㇝ཤ㺘⽪䖜ᖅᯩੁˈⴤ䀂㇝ཤ㺘᰾ޕᒿࡇ䚇Ր㓴ᡀݳԦ઼ Southern ᵲӔ࠶᷀ѝⲴ䲀ࡦᙗ䞦࠷ս⛩ˈᨂޕമк䜘㺘⽪ᨂ 㘬ᒿࡇⲴᔰս㖞઼ᯩੁDŽമл䜘ᡰ⽪Ѫ䲀ࡦᙗ䞦Ⲵ亴ᵏ䞦࠷⡷⇥བྷሿˈᵜ⽪മᒦᵚ᤹∄ֻ㔈ࡦˈഐ↔䚇ՐݳԦⲴח㊣สഐ㓴⦹ ս㖞઼ T-DNA ᧒䪸བྷሿѪ䘁լ٬DŽ
48 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 1˅᧒䪸 1 ઼᧒䪸 4 ⲴᵲӔ㔃᷌˄ᨂޕ৺ᤧ䍍ᮠỰ⍻˅ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆᰦˈ൘~6.1 kb ઼~4.1 kb ༴ӗ⭏ⓀᙗᵲӔᶑᑖ˄മ 14ˈ⌣䚃 1 ઼ 8˅˗㘼⭘ Nsi I ⎸ॆᰦˈ൘~9.8 kb ઼ ⢙kb ༴ӗ⭏ⓀᙗᵲӔᶑᑖ˄മ 14ˈ⌣䚃 3 ઼ 10˅DŽ䘉ӋᵲӔؑਧ൘⍻䈅 4.3~ ᑨ㿴ሩ➗⌣䚃ѝ൷ᆈ൘ˈഐ↔䇔Ѫ䘉ӋؑਧᱟⓀᙗⲴ㛼ᲟᵲӔˈᒦ䶎⢩ᔲᙗ઼ DNADŽ䘉Ӌؑਧᖸਟ㜭ᱟ⭡᧒䪸 1 оⓀ Hsp70 ޕൠ䪸ሩ MON 87427 ѝⲴᨂ ᒿࡇⲴᵲӔᕅ䎧ⲴˈഐѪ䈕᧒䪸ਜ਼ᴹаሿ䜘࠶ Hsp70 ਜ਼ᆀ˄മ ˅DŽ⭡Ҿ᧒ 䪸 1 ӵ㾶ⴆҶ Hsp70 ਜ਼ᆀѝᖸሿа䜘࠶ᒿࡇˈᵲӔؑਧሩ䖳ᕡDŽ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо亴ݸ⭘ Sph I ⎸ॆⲴ PV-ZMAP1043 䍘㋂ DNA ਸ˄മ 14ˈ⌣䚃 7˅ˈ䲔к䘠ⓀᙗᵲӔᶑᑖཆˈ 䘈൘~7.1 kb ઼~1.8 kb ༴ӗ⭏ 2 ᶑ亴ᵏᶑᑖ˄മ 13ǃ㺘 7˅DŽ䱣ᙗሩ➗ᵲӔؑਧ ⲴỰࠪˈ㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔDŽ MON 87427 สഐ㓴 DNA ԕ Nco I ⎸ॆ˄മ 14ˈ⌣䚃 2 ઼ 9˅ˈ䲔к䘠Ⓚ ᙗᵲӔᶑᑖཆˈ䘈൘~5.5 kb ઼~2.2 kb ༴ӗ⭏ 2 ᶑᑖDŽަѝˈ~5.5 kb ᶑᑖԓ㺘Ҷ 㘬สഐ㓴 DNAˈㅖਸı2.8 kb Ⲵ亴ᵏ䗩⭼⡷⇥བྷሿ˄മחT-DNA ৺ަ䛫᧕ ޕᨂ 㘬สഐ㓴חDNA Ⲵ 3'ᵛㄟ৺ަ 3'ㄟ䛫᧕Ⲵ ޕ13ǃ㺘 7˅˗~2.2 kb ᶑᑖԓ㺘Ҷᨂ മ 13ǃ㺘 7˅DŽ˄DNAˈ䈕㔃᷌ㅖਸ~2.2 kb 䗩⭼⡷⇥Ⲵ亴ᵏ٬ MON 87427 สഐ㓴 DNA ԕ Nsi I ⎸ॆ˄മ 14ˈ⌣䚃 4 ઼ 11˅ˈ䲔к䘠Ⓚ ᙗᵲӔᶑᑖཆˈ䘈൘~6.4 kbǃ~2.0 kb ઼~1.7 kb ༴ӗ⭏ 3 ᶑᑖDŽަѝˈ~1.7 kb ⡷ 㘬สഐ㓴 DNAˈㅖਸ~1.7 kbחⲴ T-DNA 5'ㄟ৺ަ䛫᧕Ⲵаሿ䜘࠶ޕԓ㺘ᨂ⇥ DNA 䜘Ⲵа䜘࠶ᒿ ޕമ 13ǃ㺘 7˅˗~2.0 kb ᶑᑖԓ㺘ᨂ˄Ⲵ䗩⭼⡷⇥亴ᵏ٬ ࡇˈㅖਸ~2.0 kb Ⲵ亴ᵏ⡷⇥བྷሿ˄മ 13ǃ㺘 7˅˗~6.4 kb ᶑᑖԓ㺘ᨂޕ T-DNA 㘬สഐ㓴 DNAˈㅖਸı1.3 kb 䗩⭼⡷⇥Ⲵ亴ᵏབྷሿ˄മח'Ⲵ 3'ㄟ৺ަ䛫᧕Ⲵ 3 13ǃ㺘 7˅DŽ മ 14 ᡰ⽪㔃᷌㺘᰾ˈ᧒䪸 1 ઼᧒䪸 4 ᡰ⏥ⴆⲴᒿࡇӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ MON 87427 ⲴঅњਟỰ⍻สഐᓗѝDŽ 2˅᧒䪸 2 ⲴᵲӔ㔃᷌˄ᨂޕ৺ᤧ䍍ᮠỰ⍻˅ ᑨ㿴ሩ➗ LH198 × HiII ઼ MON 87427 Ⲵสഐ㓴 DNA ࠶࡛⭘ Nco I ᡆ Nsi I ⢙മ 15˅ˈ൷ӗ⭏ҶаӋⓀᙗᵲӔؑਧˈ䘉ӋᵲӔؑਧ൷ᆈ൘Ҿ⍻䈅˄ॆ⎸ ᑨ㿴ሩ➗⌣䚃ѝ˄മ 15ˈ⌣䚃 1~11˅DŽഐ↔䇔Ѫ䘉ӋؑਧᱟⓀᙗⲴ㛼Ჟᵲ઼ DNADŽ䘉Ӌؑਧᖸਟ㜭ᱟ⭡᧒䪸 2 ޕӔˈᒦ䶎⢩ᔲᙗൠ䪸ሩ MON 87427 ѝⲴᨂ оⓀ hsp70 ᒿࡇⲴᵲӔᕅ䎧ⲴˈഐѪ䈕᧒䪸ਜ਼ᴹᖸབྷа䜘࠶ hsp70 ਜ਼ᆀᒿࡇ ˄മ 6ǃമ 13˅DŽ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆ˄മ 15ˈ⌣䚃 1 ઼ 8˅ˈ ൘~4.1 kb ༴ӗ⭏ⓀᙗᵲӔᶑᑖ˗ԕ Nsi I ⎸ॆ˄മ 15ˈ⌣䚃 3 ઼ 10˅ˈ൘~5.2 kb ઼~4.2 kb ༴ӗ⭏ⓀᙗᵲӔᶑᑖDŽ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘
49 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ㋂ӗ⭏Ⲵ᧒䪸⁑ᶯਸ˄മ 6ˈ᧒ 䪸 2˅ˈ䲔к䘠ⓀᙗᵲӔᶑᑖཆˈ䘈൘~1.0 kb ༴ӗ⭏亴ᵏᶑᑖ˄മ 15ˈ⌣䚃 5 ઼ 6˅DŽᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо亴ݸ⭘ Sph I ⎸ॆⲴ PV-ZMAP1043 䍘㋂ DNA ਸ˄മ 15ˈ⌣䚃 䲔к䘠ⓀᙗᵲӔᶑᑖཆˈ䘈൘~7.1 kb ༴ӗ⭏亴ᵏᶑᑖDŽ䱣ᙗሩ➗ᵲӔؑˈ˅7 ਧⲴỰࠪ㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔDŽ MON 87427 สഐ㓴 DNA ԕ Nco I ⎸ॆ˄മ 15ˈ⌣䚃 2 ઼ 9˅ˈ䲔к䘠Ⓚ ᙗᵲӔᶑᑖཆˈ䘈൘~5.5 kb ઼~2.2 kb ༴ӗ⭏ 2 ᶑᑖDŽަѝˈ~5.5 kb ᶑᑖԓ㺘Ҷ 㘬สഐ㓴 DNAˈㅖਸı2.8 kb Ⲵ亴ᵏ䗩⭼⡷⇥བྷሿ˄മחT-DNA ৺ަ䛫᧕ ޕᨂ 㘬สഐ㓴חDNA Ⲵ 3'ᵛㄟ৺ަ 3'ㄟ䛫᧕Ⲵ ޕ13ǃ㺘 7˅˗~2.2 kb ᶑᑖԓ㺘Ҷᨂ മ 13ǃ㺘 7˅DŽ˄DNAˈ䈕㔃᷌ㅖਸ~2.2 kb 䗩⭼⡷⇥Ⲵ亴ᵏ٬ MON 87427 สഐ㓴 DNA ԕ Nsi I ⎸ॆ˄മ 15ˈ⌣䚃 4 ઼ 11˅ˈ䲔к䘠Ⓚ ᙗᵲӔᶑᑖཆˈ䘈൘~2.0 kb ઼~1.7 kb ༴ӗ⭏ 2 ᶑᑖDŽަѝˈ~1.7 kb ⡷⇥ᨂޕ ⇥⡷㘬สഐ㓴 DNAˈㅖਸ~1.7 kb Ⲵ䗩⭼חT-DNA Ⲵ 5'ᵛㄟ઼аሿ䜘࠶䛫᧕Ⲵ- DNA Ⲵ䜘ᒿࡇˈㅖਸ~2.0 kb ޕമ 13ǃ㺘 7˅˗~2.0 kb ᶑᑖਜ਼ᴹᨂ˄亴ᵏ٬ Ⲵ亴ᵏ⡷⇥བྷሿ˄മ 13ǃ㺘 7˅DŽ മ 15 ᡰ⽪㔃᷌㺘᰾ˈ᧒䪸 2 ᡰ⏥ⴆⲴᒿࡇӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ MON 87427 ⲴঅњਟỰ⍻สഐᓗѝDŽ 3˅᧒䪸 3 ⲴᵲӔ㔃᷌˄ᨂޕ৺ᤧ䍍ᮠỰ⍻˅ ᑨ㿴ሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA ⭘ Nco I˄മ 16ˈ⌣ 䚃 1 ઼ 8˅ᡆ Nsi I ˄മ 16ˈ⌣䚃 3 ઼ 10˅࠶࡛⎸ॆˈྲ亴ᵏᡰᯉˈ䈕䱤ᙗሩ➗⋑ᴹӗ⭏ਟỰ⍻Ⲵ ᵲӔᶑᑖDŽ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘 ㋂ӗ⭏Ⲵ᧒䪸⁑ᶯਸ˄മ 6ˈ᧒䪸 3˅ˈ൘~1.5 kb ༴ӗ⭏亴ᵏᶑᑖ˄മ 16ˈ⌣ 䚃 5 ઼ 6˅DŽᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо亴ݸ⭘ Sph I ⎸ॆⲴ PV-ZMAP1043 䍘㋂ DNA ਸ˄മ 16ˈ⌣䚃 7˅ˈ൘~1.8 kb ༴ӗ⭏亴ᵏ ᶑᑖ˄മ 13ǃ㺘 7˅DŽᶕ㠚Ҿ䍘㋂Ⲵਖа Sph I ⡷⇥˄~7.1 kb˅ਜ਼ᴹ᧒䪸 3 ᒿࡇ Ⲵаሿ䜘࠶ˈн䗷൘ᵜ⹄ウѝⲴᵲӔ৽ᓄᶑᑖлᵚ㜭ӗ⭏ਟỰ⍻ⲴᵲӔؑਧDŽ䱣 ᙗሩ➗ᵲӔؑਧⲴỰࠪ㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔDŽ MON 87427 สഐ㓴 DNA ԕ Nco I ⎸ॆ˄മ 16ˈ⌣䚃 2 ઼ 9˅ˈ൘~2.2 kb ༴ 㘬สഐחDNA Ⲵ 3'ᵛㄟ৺ަ 3'ㄟ䛫᧕Ⲵ ޕӗ⭏ 1 ᶑᑖDŽ䈕~2.2 kb ᶑᑖԓ㺘Ҷᨂ മ 13ǃ㺘 7˅DŽMON 87427˄㓴 DNA˗䈕㔃᷌ㅖਸ~2.2 kb 䗩⭼⡷⇥Ⲵ亴ᵏ٬ สഐ㓴 DNA ԕ Nsi I ⎸ॆ˄മ 16ˈ⌣䚃 4 ઼ 11˅ˈ൘~2.0 kb ༴ӗ⭏ 1 ᶑᑖˈ↓ ྲ亴ᵏⲴаṧ˄മ 13ǃ㺘 7˅DŽ䈕~2.0 kb ᶑᑖਜ਼ᴹᨂޕ DNA Ⲵ䜘ᒿࡇDŽ മ 16 ᡰ⽪㔃᷌㺘᰾ˈ᧒䪸 3 ᡰ⏥ⴆⲴᒿࡇӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ MON 87427 ⲴঅњਟỰ⍻สഐᓗѝDŽ 4˅᧒䪸 5ǃ᧒䪸 6 ઼᧒䪸 7 ⲴᵲӔ㔃᷌˄䍘㋂僘ᷦᒿࡇỰ⍻˅ ᑨ㿴ሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA ⭘ Nco ˄മI 17ˈ⌣䚃 1 ઼ 10˅ᡆ Nsi I ˄മ 17ˈ⌣䚃 3 ઼ 12˅࠶࡛⎸ॆˈ⭘ਟԕ㾶ⴆ PV-ZMAP1043 䍘㋂僘ᷦᒿࡇⲴ
50 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᧒䪸 5ǃ᧒䪸 6 ઼᧒䪸 7 ਼ᰦᵲӔˈ↓ྲ䱤ᙗሩ➗亴ᵏᓄᴹ㔃᷌аṧˈ⋑ᴹӗ⭏ ਟỰ⍻ⲴᵲӔᶑᑖDŽᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо㓿 Sph I ⎸ॆⲴ PV-ZMAP1043 䍘㋂ਸˈ൘~7.1 kb ༴ӗ⭏亴ᵏᶑᑖ˄മ 17ˈ⌣䚃 9˗ 㺘 7˅DŽᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘㋂ӗ⭏Ⲵ᧒䪸 5 ઼᧒䪸 6˄മ 6˅Ⲵ⁑ᶯਸˈ൘~1.8 kb ઼~1.5 kb ༴ӗ⭏ 2 ᶑ亴ᵏᶑᑖ˄മ 17ˈ⌣䚃 5 ઼ 6˗㺘 7˅DŽᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘㋂ӗ⭏Ⲵ᧒䪸 7˄മ 6˅Ⲵ⁑ᶯਸˈ൘ kb ༴ӗ⭏亴ᵏᶑᑖ˄മ 17ˈ⌣䚃 7 ઼ 8˗㺘 7˅DŽ䘉Ӌ䱣ᙗሩ➗ᵲӔؑਧⲴ 1.7~ Ựࠪ㔃᷌㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔDŽ ᑨ㿴ሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA ⭘ Nco ˄മI 17ˈ⌣䚃 2 ઼ 11˅ᡆ Nsi I ˄മ 17ˈ⌣䚃 4 ઼ 13˅࠶࡛⎸ॆˈ⭘ਟԕ㾶ⴆ PV-ZMAP1043 䍘㋂僘ᷦᒿࡇⲴ ᧒䪸 5ǃ᧒䪸 6 ઼᧒䪸 7 ਼ᰦᵲӔˈ↓ྲ亴ᵏᓄᴹ㔃᷌аṧ˄㺘 7˅ˈ⋑ᴹԫօ ᵲӔᶑᑖࠪ⧠DŽ䘉а㔃᷌㺘᰾ˈMON 87427 нਜ਼ᴹԫօ PV-ZMAP1043 䍘㋂Ⲵ 僘ᷦᒿࡇDŽ
51 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ14. MON 87427ѝT-DNAᨂޕս⛩઼ᤧ䍍ᮠⲴSouthernঠ䘩˄᧒䪸1઼᧒䪸4˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢DŽ᧒䪸 1 ઼᧒䪸 4 ӵ㾶ⴆ䜘࠶ T-DNA˄മ 6 ઼മ 13˅DŽ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ࠷䞦⎸ॆⲴ DNA ṧ૱DŽ⌣䚃䈤᰾ྲ л˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ [᧒䪸 1 ઼᧒䪸 4 [~0.1 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 5 [᧒䪸 1 ઼᧒䪸 4 [~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 6 [䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 7 8 ሩ➗ LH198 × HiII˄Nco I˅ 9 MON 87427˄Nco I˅ 10 ሩ➗ LH198 × HiII˄Nsi I˅ 11 MON 87427˄Nsi I˅
㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠 52 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ15. MON 87427ѝT-DNAᨂޕս⛩઼ᤧ䍍ᮠⲴSouthernঠ䘩˄᧒䪸2˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢DŽ᧒䪸 2 ӵ㾶ⴆ䜘࠶ T-DNA˄മ 6 ઼മ 13˅DŽ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ࠷䞦⎸ॆⲴ DNA ṧ૱DŽ⌣䚃䈤᰾ྲл˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ [᧒䪸 2 [~0.1 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 5 [᧒䪸 2 [~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 6 [䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 7 8 ሩ➗ LH198 × HiII˄Nco I˅ 9 MON 87427˄Nco I˅ 10 ሩ➗ LH198 × HiII˄Nsi I˅ 11 MON 87427˄Nsi I˅
㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠 53 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ16. MON 87427ѝT-DNAᨂޕս⛩઼ᤧ䍍ᮠⲴSouthernঠ䘩˄᧒䪸3˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢DŽ᧒䪸 3 ӵ㾶ⴆ䜘࠶ T-DNA˄മ 6 ઼മ 13˅DŽ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ࠷䞦⎸ॆⲴ DNA ṧ૱DŽ⌣䚃䈤᰾ྲл˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ [᧒䪸 3 [~0.1 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 5 [᧒䪸 3 [~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 6 [䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 7 8 ሩ➗ LH198 × HiII˄Nco I˅ 9 MON 87427˄Nco I˅ 10 ሩ➗ LH198 × HiII˄Nsi I˅ 11 MON 87427˄Nsi I˅
㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠
54 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ17. MON 8742нਜ਼䍘㋂僘ᷦᒿࡇⲴSouthernᵲӔ࠶᷀˄᧒䪸5~7˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢DŽ᧒䪸 5ǃ᧒ 䪸 6 ઼᧒䪸 7 ਟԕ㾶ⴆޘ䜘 PV-ZMAP1043 䍘㋂Ⲵ僘ᷦᒿࡇ˄മ 6˅DŽ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ࠷䞦⎸ॆ Ⲵ DNA ṧ૱DŽ⌣䚃䈤᰾ྲл˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 5 ઼᧒䪸 6[~0.1ؽสഐ㓴ᖃ䟿]ਸ 5 ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 5 ઼᧒䪸 6[~1.0ؽสഐ㓴ᖃ䟿]ਸ 6 ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 7 [~0.1 ؽสഐ㓴ᖃ䟿]ਸ 7 ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 7 [~1.0 ؽสഐ㓴ᖃ䟿]ਸ 8 ሩ➗ LH198 × HiII˄Nco I˅䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿]ਸ 9 10 ሩ➗ LH198 × HiII˄Nco I˅ 11 MON 87427˄Nco I˅ 12 ሩ➗ LH198 × HiII˄Nsi I˅ 13 MON 87427˄Nsi I˅ ㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠
55 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2.3 ⴤⲺะഖф䖳։ᶺᔰഴ䉧θ䖳։Ⲻ〦ȽᶛⓆȽ㔉ᶺȽ⢯ᙝૂ ᆿޞᙝθऻᤢ䖳։ᱥੜᴿ㠪⯻ᙝԛᱥੜਥ㜳╊Ѱᴿ㠪⯻ᙝ MON 87427 Ⲵ䖜ॆ䖭փѪ PV-ZMAP1043 䍘㋂DŽPV-ZMAP1043 䍘㋂Ⲵབྷሿ 㓖Ѫ 8.9 kbˈवਜ਼࠶࡛⭡ᐖǃਣ䗩⭼࠶ᔰⲴ T-DNADŽT-DNA वਜ਼ањᆼᮤⲴ cp4 epsps สഐ㺘䗮ⴂˈ䈕สഐ㺘䗮ⴂवᤜ e35S ࣘᆀǃhsp70 Ⲵਜ਼ᆀǃCTP2 ਦ㔯փ䖜〫㛭ᒿࡇǃcp4 epsps 㕆⸱ᒿࡇ઼ nos 㓸→ᆀDŽPV-ZMAP1043 䍘㋂ᡰव 䜘䚇ՐݳԦ≷ᙫҾ㺘 8ˈަ䍘㋂മ䉡㿱മ 6DŽޘਜ਼Ⲵ PV-ZMAP1043 䍘㋂ᱟӪᐕᶴᔪਸᡀⲴˈަ僘ᷦᶕⓀҾབྷ㛐ᵶ㧼DŽབྷ㛐ᵶ㧼 ൘⧟ຳ䟼ᰐᡰн൘ˈҏᆈ൘ҾӪ㊫ㅹ㜺Ἶࣘ⢙Ⲵ⎸ॆ㌫㔏ѝ˄Jefferson et al., 1986˅DŽ⭡Ҿབྷ㛐ᵶ㧼Ⲵ䘉⭏⢙ᆖ⢩ᙗˈа㡜䇔Ѫ⋑ᴹᗵ㾱ਖཆ䘋㹼仍ཆᆹޘ ᙗ࠶᷀ᡆᇎ傼DŽ䈕㺘䗮䖭փнާᴹ仍ཆⲴ㠤⯵ᙗˈҏнਟ㜭╄ਈᡀѪާᴹ㠤⯵ᙗ Ⲵ䖭փˈሩӪ㊫઼ࣘ⢙ᱟᆹޘⲴDŽ PV-ZMAP1043 䍘㋂кⲴᐖ䗩⭼ᒿࡇ઼ਣ䗩⭼ᒿࡇᶕⓀҾṩⱼߌᵶ㧼 ˄Agrobacterium tumefaciens˅˄Barker et al., 1983; Depicker et al., 1982; Zambryski et al., 1982˅DŽ⇿њ䗩⭼ᒿࡇवਜ਼ᴹ 24-25 bp ൘䖜ॆᰦਟԕਁ⭏ DNA ӔᦒⲴ 㕪ս⛩DŽᐖਣ䗩⭼ᒿࡇሶ T-DNA ઼䍘㋂僘ᷦᒿࡇ࠶ᔰDŽ൘ PV-ZMAP1043 䍘 ㋂Ⲵ僘ᷦᒿࡇ४ฏवᤜᴹ㓶㧼༽ࡦ䎧⛩˄ori V ઼ ori-pBR322˅ǃ㓶㧼䘹ᤙḷ ᤱ൘བྷ㛐ᵶ㧼ѝⲴᤧ䍍ᮠⲴᕅᆀ㳻ⲭ䱫䙿⢙Ⲵ㕆⸱४˄rop˅˄㺘؍઼ 䇠สഐ aadA DŽн䇪ᱟ䘉ӋսҾ僘ᷦᒿࡇк䚇ՐݳԦ䘈ᱟ僘ᷦкⲴަᆳᒿࡇ൷⋑ᴹᮤਸ䘋˅8 ⦹㊣สഐ㓴˄മ 17˅DŽ
56 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘8. PV-ZMAP1043䍘㋂ᡰਜ਼䚇ՐݳԦᾲ䘠 䍘 ㋂ ѝ Ⲵ ˅⥞䚇ՐݳԦ ࣏㜭᧿䘠˄৲㘳᮷ ս㖞˄bp˅ T-DNA ṩⱼߌᵶ㧼 T-DNA ᐖ䗩⭼ᒿࡇˈ৲о T-DNA 䖜઼〫ᮤਸ B1-ᐖ䗩⭼ 1-442 ˄Barker et al., 1983˅ 䰤ᨂᒿࡇ 443-483 DNA ݻ䲶઼ᶴᔪ䴰㾱 P2-e35S वਜ਼ᴹ䟽༽໎ᕪ४ฏ˄Kay et al., 1987˅Ⲵ㣡ὠ㨌㣡ਦ⯵∂ 484-1104 ˄CaMV˅35S RNA Ⲵࣘᆀ˄Odelletal.,1985˅ 䰤ᨂᒿࡇ 1105-1125 DNA ݻ䲶઼ᶴᔪ䴰㾱 ⦹㊣✝◰㳻ⲭ 70 สഐⲴㅜањਜ਼ᆀ˄Brown and Santino, I3-hsp70 1126-1929 1997˅ 䰤ᨂᒿࡇ 1930-1953 DNA ݻ䲶઼ᶴᔪ䴰㾱 㕆⸱ᤏই㣕 EPSPS 䞦ਦ㔯փ䖜〫㛭४ฏⲴสഐ ShkG Ⲵ䶦 TS4-CTP2 1954-2181 ḷᒿࡇ˄Herrmann, 1995; Klee et al., 1987˅ˈᕅሬ CP4 EPSPS 䖜〫ࡠਦ㔯փ 㓿ᇶ⸱ᆀՈॆⲴṩⱼߌᵶ㧼 CP4 㧼Ṛ aroA สഐ㕆⸱ᒿࡇˈ CS5- 2182-3549 㕆⸱ CP4 EPSPS 㳻ⲭ˄Barry et al., 2001; Padgette et al., cp4 epsps 1996˅ 䰤ᨂᒿࡇ 3550-3555 DNA ݻ䲶઼ᶴᔪ䴰㾱 ṩⱼߌᵶ㧼㜝㜲⻡ਸ䞦สഐ˄nos˅3'ㄟ䶎䖜ᖅ४ˈ㓸→䖜 T6-nos 3556-3808 ᖅᒦфᕅሬ mRNA Ⲵཊ㚊㞪㤧䞨ॆ˄Bevan et al., 1983˅ 䰤ᨂᒿࡇ 3809-3835 DNA ݻ䲶઼ᶴᔪ䴰㾱 ṩⱼߌᵶ㧼 T-DNA ᐖ䗩⭼ᒿࡇˈ৲о T-DNA 䖜઼〫ᮤਸ B-ਣ䗩⭼ 3836-4192 ˄Depicker et al., 1982; Zambryski et al., 1982˅ 䍘㋂僘ᷦ DNA 䰤ᨂᒿࡇ 4193-4328 DNA ݻ䲶઼ᶴᔪ䴰㾱 䖜ᓗᆀ Tn7 Ⲵ≘ส㤧㊫ᣇ⭏㍐䫍ॆ䞦˄3''(9)-O-Ṩ㤧䞨䖜〫 aadA 4329-5217 䞦˅Ⲵࣘᆀǃ㕆⸱ᒿࡇ઼ ㄟ䶎䖜ᖅ४˄Fling et al., 1985˅ˈ ਟԕ֯㓶㧼ᣇᇴ᭮㓯㧼㍐઼䬮䴹㍐ 䰤ᨂᒿࡇ 5218-5747 DNA ݻ䲶઼ᶴᔪ䴰㾱 OR7-ori- pBR322 䍘㋂Ⲵ DNA ༽ࡦ䎧⛩ˈ㔤ᤱབྷ㛐ᵶ㧼˄E. coli˅ 5748-6336 pBR322 ѝ䍘㋂Ⲵ༽ࡦ˄Sutcliffe, 1979˅ 䰤ᨂᒿࡇ 6337-6763 DNA ݻ䲶઼ᶴᔪ䴰㾱 ColE1 䍘㋂ѝᕅᆀ㳻ⲭ䱫䙿⢙Ⲵ㕆⸱ᒿࡇˈ㔤ᤱབྷ㛐ᵶ㧼 CS-rop 6764-6955 ˄E. coli˅ѝ䍘㋂Ⲵᤧ䍍ᮠ˄Giza and Huang, 1989˅ 䰤ᨂᒿࡇ 6956-8463 DNA ݻ䲶઼ᶴᔪ䴰㾱 ᒯᇯѫ㤳ത䍘㋂ RK2 Ⲵ༽ࡦ䎧ս⛩ˈ㔤ᤱߌᵶ㧼 OR-ori V 8464-8860 ˄Agrobacterium˅ѝ䍘㋂༽ࡦ˄Stalker et al., 1981˅ 䰤ᨂᒿࡇ 8861-8946 DNA ݻ䲶઼ᶴᔪ䴰㾱 1 B–䗩⭼ᒿࡇ˗2 P–ࣘᆀ˗3 I–ਜ਼ᆀ˗4 TS –䖜〫㳻ⲭᒿࡇ˗5 CS –㕆⸱ᒿࡇ˗6 T– 㓸→ᆀ˗7 OR –༽ࡦ⛩ 57 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ၻᄯЌద့ْଫխԅᆇॸ 2.4 ։⭕⢟Ⲻ〦בࣞᆆૂ㓾↘ᆆⲺཝቅȽࣕ㜳ެ 2.4.1 MON 87427 ѝ cp4 epsps สഐⲴ㺘䗮⭡ e35S ࣘᆀ傡ࣘDŽe35S ࣘᆀབྷሿ Ѫ 621 bpDŽe35S ࣘᆀᱟवਜ਼ᴹ䟽༽໎ᕪ४ฏ˄Kay et al., 1987˅Ⲵ㣡ὠ㨌㣡ਦ ⯵∂˄CaMV˅35S RNA Ⲵࣘᆀ˄Odell et al., 1985˅DŽ35S ࣘᆀਜ਼ᴹ 2 њ㔃ᶴ ฏ˄㔃ᶴฏ A ઼㔃ᶴฏ B˗㘼 e35S 䲔Ҷ㔃ᶴฏ A ԕཆवᤜᴹ 2 њ㔃ᶴฏ Bˈԕ ໎ᕪ傡ࣘ㺘䗮Ⲵ㜭࣋DŽe35S ࣘᆀⲴ㔃ᶴਟ৲㿱മ 3DŽ൘ e35S ࣘᆀਾ䶒ᱟа њᶕ㠚Ҿ⦹㊣✝◰㳻ⲭ 70 สഐ˄hsp70˅Ⲵㅜањਜ਼ᆀDŽ䈕 e35S ࣘᆀ-hsp70 ਜ਼ᆀ㓴ਸਟԕ⢩ᔲᙗ᧗ࡦ CP4 EPSPS 㳻ⲭ൘ MON 87427 Ⲵ㩕ޫಘᇈ઼䳼ᙗ⭏ ⇆ಘᇈ㺘䗮ˈ㘼൘䳴ᙗ⭏⇆ಘᇈн㺘䗮ᡆ㺘䗮䟿ᖸቁDŽഐ↔ਟԕ௧ᯭ㥹⭈㟖䲔㥹 ࡲᵰ䳴ˈࡋ䙐Ӫᐕ䳴ᙗн㛢ˈ⭘Ҿࡦ઼ᆀ⭏ӗDŽ MON 87427 Ⲵ cp4 epsps สഐⲴ㓸→ᆀѪṩⱼߌᵶ㧼㜝㜲⻡ਸ䞦สഐ˄nos˅ 3'ㄟ䶎䖜ᖅ४ˈབྷሿѪ 253 bpˈਟԕ㓸→䖜ᖅᒦфᕅሬ mRNA Ⲵཊ㚊㞪㤧䞨ॆ ˄Bevan et al., 1983˅DŽ ։⭕⢟Ⲻ〦בḽ䇦ะഖૂᣛะഖⲺཝቅȽࣕ㜳ެ 2.4.2 PV-ZMAP1043 䍘㋂Ⲵᨂޕ४ฏ˄T-DNA˅нवᤜԫօḷ䇠สഐ઼ᣕส ഐDŽն⭡ҾⴞⲴสഐ cp4 epsps ਟԕ֯䖜ॆ㓶㜎઼䖜สഐἽṚᣇ㥹⭈㟖ˈഐ㘼൘ 䖜ॆ䗷〻ѝਟԕѪ䘹ᤙḷ䇠DŽcp4 epsps สഐབྷሿѪ 1368 bpˈᶕⓀҾ൏༔ṩⱼ ߌᵶ㧼Ⲵ CP4 㧼Ṛ˄Agrobacterium tumefaciens sp.CP4˅DŽ ։⭕⢟בᆹ㺞䗴䈹ᓅࡍⲺ〦ެᶛⓆδྸӰᐛਾᡆᡌެ 2.4.3 〦ε ४ฏ˄T-DNA˅䘈वᤜަᆳаӋ䈳᧗ᒿࡇᡆݳޕPV-ZMAP1043 䍘㋂Ⲵᨂ ԦDŽާփ᧿䘠ྲл˖ T-DNA Ⲵᐖਣ䗩⭼ᒿࡇ˖ᐖ䗩⭼ᒿࡇѪབྷሿ 442 bpˈਣ䗩⭼ᒿࡇབྷሿѪ 357 bpDŽ䘉єњ䗩⭼ᒿࡇ൷ᶕⓀҾṩⱼߌᵶ㧼 T-DNA Ⲵ䗩⭼ᒿࡇˈ৲о T-DNA 䖜઼〫ᮤਸDŽ ⦹㊣✝◰㳻ⲭ hsp70 ਜ਼ᆀ˖བྷሿѪ 804 bpDŽᶕⓀҾ⦹㊣✝◰㳻ⲭ 70 สഐ ˄hsp70˅Ⲵㅜањਜ਼ᆀDŽਜ਼ᆀⲴ⭘ᵪࡦⴞࡽ䘈нॱ࠶ᾊˈնਟԕ㛟ᇊ Ⲵᱟਜ਼ᆀՊ䙊䗷࢚᧕ᵪࡦ৲оἽ⢙Ⲵสഐ㺘䗮䈳᧗DŽ൘ MON 87427 ѝ hsp70 ਜ਼ᆀ઼ e35S ࣘᆀޡ਼䈳᧗ CP4 EPSPS ൘ MON 87427 Ⲵ㩕ޫಘᇈ઼䳼ᙗ⭏ ⇆ಘᇈ㺘䗮ˈ㘼൘䳴ᙗ⭏⇆ಘᇈн㺘䗮DŽ CTP2 ਦ㔯փ䖜〫㛭㕆⸱ᒿࡇ˖བྷሿѪ 228 bpDŽᶕⓀҾᤏই㣕 EPSPS 䞦ਦ㔯 փ䖜〫㛭४ฏⲴสഐ ShkG Ⲵ䶦ḷᒿࡇˈަ㕆⸱Ⲵਦ㔯փ䖜䘀㛭 CTP2 ᕅሬ CP4 EPSPS 㳻ⲭੁਦ㔯փ䖜䘀DŽ
58 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2.5 䖢ะഖᯯ⌋ MON 87427 ᱟ䙊䗷ߌᵶ㧼ӻሬ䖜ॆਇփ⦹㊣ᒬ㜊㧧ᗇⲴˈ䖜ॆ䖭փѪӪ ᐕᶴᔪⲴ PV-ZMAP1043DŽ䟷⭘Ⲵ䖜ॆᯩ⌅о Sidorov ઼ Duncan˄2009˅ӻ㓽 Ⲵᯩ⌅਼DŽ䖜ॆਇփ LH198 × HiII ᱟњᵲӔˈަѝ LH198 ާᴹ㢟ྭⲴߌ㢪 ḃ∄䖳ᝏ৸᱃Ҿ࠶ॆ⭏ˈᱟᑨ⭘Ⲵ⦹㊣䖜ॆਇփDŽץᙗ⣦ˈ㘼 HiII ሩߌᵶ㧼 Ӿ LH198 × HiII ਇփ⦹㊣Ⲵ䳼ょਆᒬ㜊ˈоᩪᑖ䖜ॆ䖭փⲴߌᵶ㧼ޡษ ޫˈ❦ਾሶ㓿оߌᵶ㧼ޡษޫⲴᒬ㜊䖜〫㠣ਜ਼ᴹ㥹⭈㟖ǃ㗗㣴䶂䴹㍐઼⼧䞨≒ Ҽ䫐Ⲵㆋ䘹ษޫสк㔗㔝ษޫDŽㆋ䘹ษޫสਟԕᣁࡦᵚ䖜ॆ㓶㜎઼↻։ߌᵶ㧼 Ⲵ⭏䮯ˈӾ㘼ሩ䖜ॆ㓶㜎䘋㹼䘹ᤙDŽаᰖ൘ㆋ䘹ษޫสᗇࡠՔ㓴㓷ˈሶՔ ⧠䘋ṩ઼㤾Ⲵ࠶ॆDŽሶᐢ㓿⭏ṩф㺘׳㓴㓷䖜〫㠣࠶ॆษޫส֯ަ㔗㔝⭏䮯ᒦ ᑨⲴ R0 ԓἽṚ〫ṭࡠ൏༔ѝˈ֯䖜ॆἽṚਟԕ䘋а↕⭏䮯ˈᒦ䘋а↕䇴ԧDŽ↓
⭡Ҿ R0 ԓἽṚᐢ㓿൘ਜ਼ᴹ㥹⭈㟖ⲴษޫสⲴ䘋㹼䗷ㆋ䘹ˈഐ↔ R0 ԓἽṚ ާᴹᣇ㥹⭈㟖Ⲵ⢩ᙗDŽԕ LH198 Ѫ䖞എӢᵜˈሩ R0 ԓἽṚ䘋㹼䘎㔝 4 ԓⲴഎ ӔDŽഎӔਾԓ㾱⭘㥹⭈㟖˄0.84 kg a.e./ha˅䘋㹼ㆋ䘹઼䇴ԧDŽሶᣇ㥹⭈㟖Ⲵഎ ӔਾԓἽṚ㠚Ӕˈԕ㧧ᗇ㓟ਸњփἽṚˈᒦ⭘ᇊ䟿 PCR ᯩ⌅ሩ㓟ਸἽṚ䘋㹼䢤 ᇊDŽᴰ㓸ᗇࡠ㺘ර㢟ྭⲴ MON 87427DŽ
59 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2.6 Ќద༝ॹζӒԅᆇॸ 2.6.1 ᨈޛᓅࡍ㺞䗴Ⲻಞᇎૂ㓺㓽θྸṯȽ㥄ȽਬȽ㣧Ƚ᷒Ƚ〃ᆆㅿ 㓴㓷઼ಘᇈѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭⲴᇊ䟿ᱟ䙊䗷䞦㚄ݽޣMON 87427 ⯛˄ELISA˅Ⲵᯩ⌅ᆼᡀⲴDŽMON 87427 Ⲵᶀᯉṧ૱ᱟӾ 2008 ᒤ൘㖾ഭ䘋㹼Ⲵ ⭠䰤䈅傼ѝ䟷䳶Ⲵˈޡ 5 њ䈅傼⛩˄࠶࡛սҾ㖾ഭ⦹㊣ѫӗ४Ⲵ䱯㛟㢢ᐎ Jackcon 䜑ǃ⡡㦧ॾᐎⲴ Jefferson 䜑ǃԺ࡙䈪ԺᐎⲴ Stark 䜑ǃঠㅜᆹ㓣ᐎⲴ Parker 䜑ԕ ৺ᐳᯟ࣐ᐎⲴ York 䜑˅ˈ⇿њ䈅傼⛩Ⲵ䈅傼Ѫ 3 ⅑䟽༽ˈਆṧ൘ᡰᴹ䈅傼 ⛩Ⲵᡰᴹ䟽༽䘋㹼DŽਆṧ઼࠶᷀Ⲵᶀᯉवᤜн਼⭏䮯䱦⇥䟷䳶Ⲵ MON 87427 㓴 㓷ṧ૱㊫රޡ䇑 19 ˖4 њ⭏㛢ᵏⲴਦ⡷˄OSL1~4˅ǃ㊭㋂ǃ㣡㊹ǃ㣡эǃൠ к䜘ᮤṚǃ㤾ਦǃ4 њ⭏㛢ᵏⲴṩ㌫˄OSR1~4˅ǃ≄⭏ṩǃ㺠㘱ṩ㌫ԕ৺ 4 њ ⭏㛢ᵏⲴᮤṚᶀᯉ˄OSWP1~4˅DŽ ELISA ࠶᷀㔃᷌≷ᙫҾ㺘 9DŽMON 87427 Ⲵн਼㓴㓷઼ಘᇈѝ CP4 EPSPS 940 μg/g dwt 㳻ⲭⲴ㺘䗮䟿ਈॆ㤳ത䖳བྷˈӾᴰሿ٬վҾỰࠪ䲀˄LOD˅ࡠᴰབྷ٬ ᗞݻ/ݻ㓴㓷ᒢ䟽˅DŽަѝਦ⡷ѝⲴᒣ൷㺘䗮䟿ᴰ儈˄㤳തӾ OSL3 Ⲵ˄ 㤳തӾ OSWP4 Ⲵ˄μg/g dwt ࡠ OSL1 Ⲵ 680 μg/g dwt˅˗ަ⅑ᱟᮤṚᒣ൷٬ 290 μg/g dwt ࡠ OSWP1 Ⲵ 500 μg/g dwt˅DŽ䲿ਾ⅑Ѫṩ㌫˄Ӿ OSR3 Ⲵ 240 73 μg/g dwt ࡠ OSR1 Ⲵ 140 μg/g dwt˅ǃൠк䜘ᮤṚ˄120 μg/g dwt˅ǃ㣡э ˄100 μg/g dwt˅ǃ≄⭏ṩ˄72 μg/g dwt˅ǃ㺠㘱ṩ㌫˄72 μg/g dwt˅ǃ㤾ਦ˄43 μg/g dwt˅ ઼㊭㋂˄4.2 μg/g dwt˅DŽ ᴹ 6 њ MON 87427 㣡㊹ṧ૱˄ᶕ㠚 3 њ䈅傼⛩˅Ⲵ CP4 EPSPS 㳻ⲭ㺘䗮䟿 վҾỰࠪ䲀˄ 60 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘 9. 2008 ᒤ൘㖾ഭ⭠䰤䟷䳶Ⲵ MON 87427 㓴㓷ṧ૱ѝ CP4 EPSPS 㳻ⲭਜ਼䟿 SD˅ LOD/LOQ4˄SD˅ ᒣ൷٬˄㓴㓷㊫ර ਁ㛢ᰦᵏ 1 ਾⲴཙ ᒣ൷٬ ᮠ˄DAP˅ 㤳ത 㤳ത 2 3 ਦ⡷ OSL1 V2-V5 20-28 100 (21) 680 (170) 0.069/0.137 75 – 140 400 – 940 OSL2 V6-V8 32-46 83 (25) 410 (130) 0.069/0.137 30 –110 130 – 560 OSL3 V10-V12 41-67 61 (19) 290 (74) 0.069/0.137 35 –95 210 – 410 OSL4 VT 54-73 95 (30) 370 (120) 0.069/0.137 17 – 140 70 – 520 ㊭㋂ R6 118-182 3.6 (0.73) 4.2 (0.89) 0.16/0.228 2.6 – 5.3 2.8 – 6.2 㣡㊹ 5 ᦸ㊹ᵏ 58-81 < LOD (NA) 㣡э ᦸ㊹ᵏ 58-76 9.4 (0.97) 100 (12) 0.121/0.137 8.1 – 11 90 – 120 ൠк䜘ᮤṚ R5 83-116 38 (14) 120 (48) 0.069/0.137 8.3 – 57 21 – 200 㤾ਦ R6 124-180 14 (6.3) 43 (27) 0.069/0.137 5.9 – 26 13 – 98 ṩ㌫ OSR1 V2-V5 22-28 18 (5.3) 140 (46) 0.033/0.068 8.1 – 27 58 – 210 OSR2 V6-V8 32-46 16 (6.8) 110 (62) 0.033/0.068 8.3 –29 48 – 240 61 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 SD˅ LOD/LOQ4˄SD˅ ᒣ൷٬˄㓴㓷㊫ර ਁ㛢ᰦᵏ 1 ਾⲴཙ ᒣ൷٬ ᮠ˄DAP˅ 㤳ത 㤳ത 2 3 OSR3 V10-V12 41-67 12 (4.3) 73 (28) 0.033/0.068 4.9 – 19 22 – 110 OSR4 VT 54-73 15 (5.7) 83 (36) 0.033/0.068 5.6 – 23 23 – 140 ≄⭏ṩ R5 83-116 15 (5.2) 72 (23) 0.033/0.068 8.6 – 24 39 – 100 㺠㘱ṩ㌫ R6 124-180 16 (8.3) 72 (37) 0.033/0.068 5.9 – 29 26 – 130 ᮤṚ OSWP1 V2-V5 22-28 50 (8.3) 500 (190) 0.069/0.137 37 – 66 310 – 840 OSWP2 V6-V8 32-46 46 (7.6) 360 (42) 0.069/0.137 33 – 58 300 – 420 OSWP3 V10-V12 41-67 43 (7.1) 380 (78) 0.069/0.137 28 – 56 230 – 500 OSWP4 VT 54-73 37 (6.3) 240 (42) 0.069/0.137 23 – 47 160 – 340 1 䟷ṧᰦ⦹㊣ᡰ༴Ⲵਁ㛢ᰦᵏDŽ ᴰ-ݻ勌䟽㓴㓷˄fwt˅ѝਜ਼ᴹⲴᗞݻᮠˈᒣ൷٬ǃḷ߶ᐞ˄SD˅઼㤳ത˄ᴰሿ٬⇿ 2 ˈᱟṩᦞᡰᴹ䈅傼⛩Ⲵṧ૱ᮠᦞ䇑㇇ᶕⲴ˄≄⭏ṩṧ૱ n=11ˈ㣡㊹ṧ૱ n=6˅བྷ٬ ަᆳṧ૱ n=14˅DŽ ݻᒢ䟽㓴㓷˄dwt˅ѝਜ਼ᴹⲴᗞݻᮠˈᒢ䟽٬ᱟṩᦞ勌䟽٬䲔ԕᒢ䟽䖜ᦒ㌫ᮠ㘼ᗇDŽ⇿ 3 4 LOQ=ᇊ䟿䲀ˈLOD=Ựࠪ䲀DŽ 5 MON 87427 㣡㊹ṧ૱Ⲵ CP4 EPSPS 㳻ⲭ≤ᒣᡆ㘵վҾỰࠪ䲀˄LODˈ ˈn=6˅ˈ ᡆ㘵ӵ⮕儈Ҿᇊ䟿䲀˄LOQˈ ˈn=6˅DŽ 62 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2.6.2 ᨈޛᓅࡍ㺞䗴䠅ެ࠼᷆ᯯ⌋ ྲ 2.6.1 䜘࠶᧿䘠ⲴˈMON 87427 㓴㓷઼ಘᇈѝ CP4 EPSPS 㳻ⲭⲴ㺘䗮 䟿ᱟ䙊䗷 ELISA ᯩ⌅࠶᷀⺞ᇊⲴDŽᵜ⹄ウᡰ⭘ ELISA ࠶᷀ᯩ⌅ྲл˖ 1˅ᶀᯉ༴⨶৺㳻ⲭᨀਆ MON 87427 Ⲵ ELISA ࠶᷀ᶀᯉᱟӾ 2008 ᒤ൘㖾ഭ䘋㹼Ⲵ 5 њ䈅傼⛩⭠䰤 䈅傼ѝ䟷䳶Ⲵˈ䘉 5 њ䈅傼⛩वᤜ˖䱯㛟㢢ᐎ Jackcon 䜑ǃ⡡㦧ॾᐎⲴ Jefferson 䜑ǃԺ࡙䈪ԺᐎⲴ Stark 䜑ǃঠㅜᆹ㓣ᐎⲴ Parker 䜑ԕ৺ᐳᯟ࣐ᐎⲴ York 䜑˅DŽ⇿њ䈅傼⛩Ⲵ䈅傼Ѫ 3 ⅑䟽༽ˈਆṧ൘ᡰᴹ䈅傼⛩Ⲵᡰᴹ䟽༽䘋㹼DŽਆṧ ᶀᯉवᤜ˖4 њ⭏㛢ᵏⲴਦ⡷˄OSL1~4˅ǃ㊭㋂ǃ㣡㊹ǃ㣡эǃൠк䜘ᮤṚǃ 㤾ਦǃ4 њ⭏㛢ᵏⲴṩ㌫˄OSR1~4˅ǃ≄⭏ṩǃ㺠㘱ṩ㌫ԕ৺ 4 њ⭏㛢ᵏᮤṚ ˄OSWP1~4˅DŽ ᡰᴹᇎ傼ᶀᯉ䟷䳶ਾˈޘ䜘䘀㠣ᆏኡ䜭ޜਨᙫ䜘DŽ䲔㣡㊹ཆˈަᆳᶀᯉ㓿 ᆈ൘-80°C ߠ㇡༷⭘DŽ؍䜘ᇎ傼ᶀᯉޘ䗷⼘⺾༴⨶DŽ ᡰᴹ䴰㾱ᨀਆ CP4 EPSPS 㳻ⲭⲴ㓴㓷ᶀᯉ≷ᙫҾ㺘 10DŽަѝ㊭㋂ᶀᯉⲴ ᨀਆ䟷⭘ਜ਼ᴹ L-㔤⭏㍐ C ઼ 10 mM 㝡≗㛶䞨Ⲵ TBA 㕃ߢ⏢˄0.1 M Tris + 0.1 M ® Na2B4O7 • 10H2O + 0.005 M MgCl2 •6H2O + 0.05% (v/v) Tween -20, pH 7.8 + 0.2% (w:v) L-ascorbic acid + 10 mM 㝡≗㛶䞨˅˗㘼ަᆳᶀᯉⲴ㳻ⲭᨀਆ䟷⭘ਜ਼ ᴹ 0.1%⢋㹰㳻ⲭ˄BSA˅Ⲵ pH 7.4 ⼧䞨ⴀ㕃ߢ⏢˄0.001 M KH2PO4 + 0.01 M Na2HPO4 •7H2O + 0.137 M NaCl + 0.0027 M KCl + Tween 20 (1× PBST) + 0.1% (w/v) BSADŽާփᨀਆᯩ⌅Ѫ˖ਆࠪᓄⲴ㓴㓷ᶀᯉˈ࣐䘲䟿Ⲵᨀਆ㕃ߢ⏢ˈ㓿 ⏑ਸಘ˄Harbil Mixer˅⏑༴⨶ˈ⭘㹰䗷└㻵㖞˄Fisher Scientific, ᆈҾ-80°C ߠ㇡༷⭘DŽ؍Pittsburgh, PA˅└ᦹнⓦ䀓⢙ˈሶᨀਆ⏢࠶㻵ᒦ 㺘10. н਼㓴㓷Ⲵ㳻ⲭᨀਆᯩ⌅ 㓴㓷㊫ර 㓴㓷:㕃ߢ⏢∄ ᨀਆ㕃ߢ⏢ 1 ਦ⡷ 2 1:100 1× PBST with 0.1% (w/v) BSA ㊭㋂ 1:100 1× TBA with 10 mM DCA 㣡㊹ 1:100 1× PBST with 0.1% (w/v) BSA 㣡э 1:100 1× PBST with 0.1% (w/v) BSA ṩ㌫ 3 1:50 1× PBST with 0.1% (w/v) BSA 〨⿶ 4 1:100 1× PBST with 0.1% (w/v) BSA 1 ൘⼘⺾ᶀᯉѝ࣐䘲䟿㕃ߢ⏢ˈ⭘ Harbil ⏑ਸಘ༴⨶ˈ⭘㹰䗷└ಘ䗷└ᨀਆ⏢DŽ 2 वᤜഋњᰦᵏⲴᶀᯉ˄OSL1ǃOSL2ǃOSL3 ઼ OSL4˅DŽ 3 वᤜ OSR1ǃOSR2ǃOSR3 ઼ OSR4 ᰦᵏⲴṩԕ৺≄⭏ṩ઼㺠㘱ṩDŽ 4 वᤜൠк䜘ᮤṚǃ㤾ਦ઼ޘ⭏㛢ᵏᮤṚᶀᯉ˄OSWP1ǃOSWP2ǃOSWP3 ઼ OSWP4˅DŽ 63 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2˅CP4 EPSPS 㳻ⲭᣇփ ⭘Ҿ CP4 EPSPS Ⲵ ELISA Ự⍻Ⲵᦅ㧧ᣇփѪሿ啐অݻ䲶ᣇփ 39B6.1˄IgG 2a ਼ර㳻ⲭˈk-䖫䬮˗ᢩਧ 7022111˅DŽ䈕ᣇփᱟ⭘㳻ⲭ A ⩬㜲㌆ࠍ㜦Ӣ઼ቲḡ Ӿሿ啐㞩≤ѝ㓟ॆᗇࡠⲴDŽ⭘࠶ݹݹᓖ⌅⍻ᗇ㓟ॆⲴ IgG ⎃ᓖѪ 2.3 mg/mlDŽ 39B6.1 অݻ䲶ᣇփ⭡ Strategic Biosolutions˄Newark, DE˅ࡦ༷DŽ㓟ॆⲴᣇփᆈ Ҿਜ਼ᴹ 20 mM ⼧䞨䫐ǃ150 mM ≟ॆ䫐઼ 15 ppm Proclin 300˄Sigma-Aldrich, St. 㚄ᴹ HRP Ⲵኡ㖺ᣇ CP4 EPSPSڦLouis, MO˅Ⲵ㕃ߢ⏢˄pH 7.2˅ѝDŽỰ⍻䈅ࡲѪ ᣇփˈҏ㻛〠Ѫᣇ-㳻ⲭ 4˄Sigma-Aldrich, ᢩਧ P-5867˅DŽ 3˅CP4 EPSPS 㳻ⲭⲴ ELISA ࠶᷀ ሶሿ啐ᣇ CP4 EPSPS ᣇփ⭘व㻛㕃ߢ⏢˄15 mM Na2CO3ˈ35 mM NaHCO3 ઼ 150 mM NaClˈpH 9.6˅〰䟺㠣㓸⎃ᓖ 2.0 μg/mlˈപᇊ൘ 96 ᆄᗞᆄᶯкˈ❦ ਾ൘ 4ºC ߠ㇡ѝᆥ㛢 8hԕкDŽԕл↕䈅傼ѻࡽˈ⭘ 1× PBST ߢ⍇ᗞᆄ ᶯDŽੁ⇿њᆄѝ࣐ޕ 100 μl CP4 EPSPS 㳻ⲭḷ߶ᡆṧ૱ᨀਆ⏢ˈ37ºC лᆥ㛢 1hDŽ ੁᆄѝ࣐ޕ 100 μl ᣇ CP4 EPSPS 䗷≗ॆ䞦㔃ਸ⢙ˈԕỰ⍻ᦅ㧧ࡠⲴ μl 100 ޕμl TMB ֯ᶯᱮ㢢DŽᆄѝ࣐ 100 ޕCP4 EPSPS 㳻ⲭDŽੁᆄѝ࣐ 6MH3PO4 㓸→䞦⍫৽ᓄDŽCP4 EPSPS 㳻ⲭⲴᇊ䟿ᱟ䙊䗷൘ 0.456~14.6 ng/ml 㤳തˈ࡙⭘᧘⌅Ӿ㔈ࡦⲴḷ߶ᴢ㓯к䈫ਆDŽ 4˅ਜ਼≤䟿࠶᷀ ⢩ᇊⲴ㓴㓷㊫ර൘⇿њ䈅傼⛩ᔪ・൷аⲴ TSSP˄⢩ᇊ㓴㓷䈅⛩ᮠᦞ⊐ˈ tissue-specific site pool˅DŽ䲔㣡㊹ཆˈ⇿㓴㓷൷㾱ᔪ・ TSSPDŽ䲔㣡㊹ཆˈᡰᴹ Ⲵ㓴㓷Ⲵਜ਼≤䟿࠶᷀൷䟷⭘ IR-200 ⒯ᓖ࠶᷀Ԛᶕ࠶᷀˄Denver Instrument Company, Arvada, CO˅DŽ TSSP Ⲵᒣ൷≤࠶ਜ਼䟿Ӿй⅑䟽༽࠶᷀ѝ䇑㇇㘼ᗇDŽ 䙊䗷л䶒Ⲵ䇑㇇ޜᔿᗇࠪ⇿њ TSSP Ⲵᒢ䟽ᦒ㇇㌫ᮠ˄DWCF˅ [TSSP ⒯ᓖ / 100 %ᒢ䟽ᦒ㇇㌫ᮠ =1–[ᒣ൷٬ ᒢ䟽䖜ᦒ㌫ᮠ⭘Ҿሶԕ 㓴㓷勌䟽˄fwt˅ѪঅսⲴ㳻ⲭ≤ᒣ࠶᷀㔃 ᷌䖜ᦒᡀԕ 㓴㓷ᒢ䟽˄dwt˅অսˈ䇑㇇ޜᔿྲл˖ g/g fwt g/g dwt = DWCF 5˅ᮠᦞ࠶᷀ ԕ SPECTRAmax Plus˄Molecular Devices, Sunnyvale, CA˅ᡆ SPECTRAmax Plus 384˄Molecular Devices, Sunnyvale, CA˅ᗞオᶯ࠶ݹݹᓖ䇑ˈሩᡰᴹⲴ CP4 EPSPS Ⲵ ELISA ৽ᓄᒣᶯ࡙⭘ৼ䟽⌒䮯Ự⍻ᯩ⌅䘋㹼࠶᷀DŽ⍻ᇊᡰᴹ㳻ⲭ ᰦ৲➗൘⌒䮯Ѫ 620~650 nm ᰦⲴ䈫ᮠᶕ⺞ᇊ਼ˈ൘ 450 nm ⌒䮯༴Ⲵݹ੨᭦٬ ᡰᴹ㳻ⲭⲴ⎃ᓖDŽ䟷⭘ Molecular Devices SOFTmax PRO GXP Version 5.0.1 䘋㹼 ᮠᦞ䖜ᦒDŽṩᦞḷ߶㳻ⲭⲴ⎃ᓖ৺੨ݹ⦷䈫ᮠᤏਸᡀഋ৲ᮠሩᮠᴢ㓯DŽӾḷ߶ ᴢ㓯᧘䇑㇇㳻ⲭ⎃ᓖਾˈᗇࡠ㓴㓷ѝ㳻ⲭⲴਜ਼䟿˄ng/ml˅ˈ⭘ṧ૱〰䟺ഐᆀ 㓴㓷/㕃ߢ⏢〰䟺ؽᮠ䖜ᦒᡀԕ # PѪঅսⲴᮠᦞDŽ䲔㣡㊹㓴㓷ཆˈᓄ઼ 64 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⭘ᒢ䟽ᦒ㇇㌫ᮠሶ㳻ⲭⲴ勌䟽ਜ਼䟿˄ # ˅䖜ᦒᡀᒢ䟽ਜ਼䟿˄ ˅DŽ࡙ ⭘ Microsoft Excel 2007 䖟Ԧ䇑㇇⦹㊣㓴㓷ѝ CP4 EPSPS 㳻ⲭⲴਜ਼䟿≤ᒣDŽ ྲ᷌ ELISA Ự⍻㔃᷌䎵ࠪ亴ᵏ㤳തˈࡉሶ䟽ᯠᨀਆ 2 њ㳻ⲭṧ૱ᒦ䘋㹼 Ự⍻㔃᷌↓⺞ਟ䶐DŽ؍⺞ELISA ࠶᷀ˈԕ MON 87427 н਼㓴㓷઼ಘᇈѝ CP4 EPSPS 㳻ⲭⲴ㺘䗮䟿䈖㿱 2.6.1 ѝ㺘 9DŽ 2.6.3 ᨈޛᓅࡍ㺞䗴Ⲻどᇐᙝ MON 87427 ᱟ䙊䗷ߌᵶ㧼ӻሬ䖜ॆᯩ⌅ሶ PV-ZMAP1043 䍘㋂Ⲵ T-DNA ሬ ޕࡠਇփ⦹㊣ LH198 × HiII ѝ㧧ᗇⲴDŽLH198 × HiII ᱟњᵲӔˈަѝӢᵜ LH198 ḃ∄䖳ᝏ৸᱃Ҿ࠶ॆ⭏ˈᱟᑨ⭘ץᴹ㢟ྭⲴߌ㢪ᙗ⣦ˈ㘼 HiII ሩߌᵶ㧼ާ Ⲵ⦹㊣䖜ॆਇփDŽ㧧ᗇⲴ R0 ԓἽṚ䙊䗷оߌ㢪ᙗ⣦䖳ྭⲴ LH198 എӔཊԓˈᒦ 㓿䘎㔝㠚ᵲˈᴰ㓸㧧ᗇ MON 87427DŽMON 87427 Ⲵ䘹㛢䗷〻৺㛢മ䉡㿱മ 18DŽ ᨂޕᒿࡇ㺘䗮っᇊᙗ࠶᷀वᤜҶ MON 87427 Ⲵ 5 њцԓᶀᯉ˄മ 18˅˖ LH198 BC3F3 цԓⲴਦ⡷઼ LH198 BC3F4ǃLH198 BC3F6ǃLH198 BC3F7 ઼ ˄LH198 BC3F7 × LH287˅F1 цԓⲴᆀ˗ሩ➗ᶀᯉѪਇփ LH198 × HiII Ⲵ ᆀDŽ䴰㾱ᤷࠪⲴᱟˈቭ㇑㠚 R0 ԓᔰˈ䖜ॆἽṚ⭘ LH198 䘋㹼ཊԓഎӔˈަ䚇 ྲ∄˄Ҿ LH198ˈն❦ਟԕ㛟ᇊަ䚇Ր㛼Ჟѝᴹਟ㜭ਜ਼ᴹ HiIIੁٮՐ㛼Ჟสᵜ ൘ BC3F4 цԓਟ㜭ਜ਼ᴹ 94%Ⲵ LH198 䚇Ր㛼Ჟ઼ 6%Ⲵ HiII 䚇Ր㛼Ჟ˅DŽ㘳㲁ࡠ ਟ㜭ᆈ൘ⲴⓀ㛼Ჟˈ䘹⭘ਇփ LH198 × HiII Ѫሩ➗DŽབྷ㛐ᵶ㧼㺘䗮Ⲵ CP4 EPSPS 㳻ⲭ˄2ng˅Ѫḷ߶৲➗㳻ⲭDŽ ࡙⭘ CP EPSPS 㳻ⲭⲴ Western ঠ䘩࠶᷀ᶕ䇱ᇎ MON 87427 ѝᨂޕᒿࡇ㺘䗮 ӗ⢙൘ཊњцԓѝⲴっᇊᙗˈWestern ࠶᷀㔃᷌㿱മ 19DŽ↓ྲ亴ᵏ㔃᷌аṧˈ MON 87427 Ⲵཊњцԓᶀᯉ൷ਟỰ⍻ࡠ CP4 EPSPS 㳻ⲭᶑᑖ˄മ 19ˈ⌣䚃 4~8˅DŽ LH198 BC3F3 цԓਦ⡷ᶀᯉⲴ Western ݽ⯛ؑਧ˄മ 19ˈ⌣ 䚃 4˅᰾ᱮᕪҾަᆳ цԓⲴᆀᶀᯉˈ䈤᰾ CP4 EPSPS 㳻ⲭ൘ਦ⡷ѝⲴ㺘䗮䟿᰾ᱮ儈Ҿᆀˈ䘉о CP4 EPSPS 㺘䗮䟿Ⲵ ELISA Ự⍻㔃᷌ᱟа㠤Ⲵ˄㺘 9˅DŽᡰᴹ䘉ӋᶑᑖⲴ䗱〫 ⦷оབྷ㛐ᵶ㧼㺘䗮 CP4 EPSPS 㳻ⲭⲴ䗱〫⦷˄മ 19ˈ⌣䚃 3˅ᱟа㠤Ⲵˈ䇱ᇎ Ҷ Western ঠ䘩ѝỰ⍻ࡠⲴ MON 87427 цԓᶀᯉݽ⯛ᶑᑖ⺞ᇎᱟ CP4 EPSPS 㳻ⲭˈᒦфњцԓᆀᶀᯉѝ㺘䗮䟿լ˄മ 19ˈ⌣䚃 5~8˅ˈ㘼ᑨ㿴ሩ➗ᶀ ᯉѝ⋑ᴹỰ⍻ࡠ CP4 EPSPS 㳻ⲭ˄മ 19ˈ⌣䚃 10˅DŽ Western ࠶᷀㔃᷌㺘᰾ˈMON 87427 Ⲵ CP4 EPSPS 㳻ⲭ൘н਼䚇Րцԓ䰤 ਟԕっᇊ㺘䗮DŽ ṯᦤр䘦䇺ԭθ৸➝ᵢࣔ⌋ㅢॷӂᶗᴿީḽࡈ࠼ะഖᬃ֒Ⲻ 2.7 ᆿޞ㊱ශ ྲкᡰ䘠ˈMON 87427 ᡰ䟷⭘Ⲵสഐ⋑ᴹ᭩ਈਇփ⭏⢙ⲴᆹޘᙗDŽṩ ᦞljߌъ䖜สഐ⭏⢙ᆹޘ䇴ԧ㇑⨶࣎⌅NJㅜҼㄐㅜॱҼᶑⲴޣࡂ࠶ḷ߶ˈ⭘ Ҿӗ⭏ MON 87427 Ⲵสഐᯩ⌅ᓄᖃᖂҾ㊫ර 2ˈণĀнᖡ૽ਇփ⭏⢙ᆹޘ ᙗⲴสഐāDŽ 65 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䘋㹼୶ъॆ ૱䖜㛢 TI: [RP×LH198 BC3F4] BC0F1 ×RP ˅TI: BC1F1 ˄࠶∄ֻ 1:1 ×RP ˅TI: BC2F1 ˄࠶∄ֻ 1:1 ! ˅TI: BC2F2 ˄࠶∄ֻ 3:1 മ18. MON 87427㛢㌫䉡മ Ӿ LH198 BC3F2 ѝ䘹ᤙаṚᣇ㥹⭈㟖ἽṚ䘋а↕㠚Ӕਟԕᗇࡠ LH198 BC3F3 ˈ䘹ᤙ LH198 BC3F3 ѝ㓟ਸփ㠚Ӕᗇࡠ LH198 BC3F4 DŽ LH198 BC3F4 цԓ⭘Ҿ࠶ᆀ⢩ᖱ⢩ᙗ࠶᷀ˈᒦ⭘䘋а↕എӔ䖜㛢ษ㛢ਟ୶ъ ॆᓄ⭘Ⲵ૱DŽLH198 BC3F3ǃLH198 BC3F4ǃLH198 BC3F6ǃLH198 BC3F7 ઼[LH198 BC3F7 × LH287] F1 цԓ˄࣐㋇ᱮ⽪˅㻛⭘Ҿཊцԓ䚇Րっᇊᙗ࠶᷀DŽ [LH198 BC3F7 × LH287] F1 цԓ⭘Ҿ䘋㹼㳻ⲭ㺘䗮䟿ǃᡀ࠶ǃ㺘රᙗ⣦ǃߌ㢪ᙗ ⣦ԕ৺⧟ຳӂㅹ࠶᷀DŽRP × LH198 BC3F4 ⲴഎӔ઼㠚Ӕਾԓᶀᯉ⭘Ҿᆏᗧቄ 䚇Րᆖ࠶᷀DŽ˄F#Ѫ㠚Ӕцԓ˗ Ѫ㠚Ӕ˗BC#ѪഎӔцԓDŽRP˖䖜㛢䖞എӢᵜDŽ˅ 66 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ19. MON 87427ཊњцԓᶀᯉѝCP4 EPSPS㳻ⲭⲴWestern࠶᷀ MON 87427 Ⲵ 4 њцԓᆀᶀᯉ઼ 1 њцԓਦ⡷ᶀᯉⲴㅹ䟿ᨀਆ⢙ ԕ৺࠶ᆀ䟿ḷ䇠䟷⭘ SDS-PAGE ࠶ˈ❦ਾ⭥䖜〫㠣 PVDF 㟌DŽሶ㟌о ˖ኡ㖺ᣇ CP4 EPSPS ᣇփᆥ㛢ˈ⭘ॆᆖਁݹ䈅ࡲᱮ㢢DŽ⌣䚃䈤᰾ྲл ⌣䚃 ᧿䘠 ࠍ㜦кṧ䟿 1 オⲭ -- 2 ࠶ᆀ䟿ḷ䇠˄Magic Marker˅ 0.5 l 3 བྷ㛐ᵶ㧼˄E. coli˅㺘䗮Ⲵ CP4 EPSPS 㳻ⲭ˄2ng˅ 2ng 4 LH198 BC3F3˄ਦ⡷˅ 20 l 5 LH198 BC3F4˄ᆀ˅ 20 l 6 LH198 BC3F6˄ᆀ˅ 20 l 7 LH198 BC3F7˄ᆀ˅ 20 l 8 (LH198 BC3F7 × LH287)F1˄ᆀ˅ 20 l 9 オⲭ -- 10 ᑨ㿴ሩ➗ LH198 × HiII˄ᆀˈᢩਧ 11214241-004˅ 20 l 67 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3. 䖢ะഖὃ⢟ᆿޞᙝ䇺ԭ 3.1 䖢ะഖὃ⢟Ⲻ䚍Ֆどᇐᙝ 1˅н਼цԓ䰤 T-DNA っᇊᙗⲴ Southern ᵲӔ࠶᷀ ޡ䘋㹼Ҷ 5 њцԓᶀᯉ T-DNA ᨂޕっᇊᙗⲴ Southern ᵲӔ࠶᷀DŽ䘉 5 њ цԓवᤜ LH198 BC3F3ǃLH198 BC3F4ǃLH198 BC3F6ǃLH198 BC3F7 ઼ ˄LH198 BC3F7 × LH287˅F1˄മ 18˅ˈަѝ LH198 BC3F4 ᱟ⭘ᶕ䘋㹼࠶ᆀ⢩ ᖱ⢩ᙗ࠶᷀Ⲵцԓ˄മ 14ǃമ 15ǃമ 16 ઼മ 17˅DŽ䖜ॆਇփᵲӔ LH198 × HiII ԕ৺ᵲӔ LH198 × LH287 Ѫ Southern ᵲӔⲴሩ➗DŽ䴰㾱ᤷࠪⲴᱟˈቭ㇑㠚 ˈҾ LH198ੁٮR0 ԓᔰ䖜ॆἽṚᔰ⭘ LH198 䘋㹼ཊԓഎӔˈަ䚇Ր㛼Ჟสᵜ ն❦ਟԕ㛟ᇊަ䚇Ր㛼Ჟѝᴹਟ㜭ਜ਼ᴹ HiII˄∄ྲ൘ BC3F4 цԓਟ㜭ਜ਼ᴹ 94%Ⲵ LH198 䚇Ր㛼Ჟ઼ 6%Ⲵ HiII 䚇Ր㛼Ჟ˅DŽ㘳㲁ࡠਟ㜭ᆈ൘ⲴⓀ㛼Ჟˈ 䘹⭘ਇփ LH198 × HiII˄㘼䶎 LH198˅Ѫሩ➗DŽ Ӿ MON 87427 Ⲵн਼цԓᶀᯉԕ৺ሩ➗ᶀᯉᨀਆ DNA˄LH198 BC3F3ǃ LH198 BC3F6 ઼ LH198 BC3F7 Ⲵ DNA ᱟӾਦ⡷ᨀਆˈަᆳцԓ DNA ᱟӾ ᆀѝᨀਆⲴ˅ˈ㓿䲀ࡦᙗ࠷䞦 Nsi I ⎸ॆ˄മ 13˅ˈ⭘ PV-ZMAP1043 䍘㋂Ⲵ ᧒䪸 1 ઼᧒䪸 4˄മ 6˅䘋㹼 Southern ᵲӔDŽྲ᷌ T-DNA ᨂޕ൘цԓ䰤н㜭ཏ っᇊ䚇ՐˈࡉՊ൘ Southern ᵲӔ㔃᷌ѝࠪ⧠䶎亴ᵏᶑᑖ˄亴ᵏᶑᑖ㿱മ 13 ઼ 㺘 7˅DŽ⭘᧒䪸 1 ઼᧒䪸 4 ᵲӔⲴ㔃᷌㿱മ 20DŽ ᰐ䇪ᱟሩ➗䘈ᱟ MON 87427 䜭൘~4.3 kb ༴Ự⍻ࡠаᶑᵲӔᶑᑖ˄മ 20ˈ 䚃 1~10˅ˈഐ↔ਟԕ䇔Ѫ䈕ᵲӔᶑᑖҾ⦹㊣Ⓚ㛼ᲟⲴᵲӔؑਧˈ㘼о⌣ MON 87427 Ⲵᨂޕ DNA ᰐޣDŽ ሩ➗ LH198 × HiII สഐ㓴 DNA ⭘ Nsi I ⎸ॆਾо᧒䪸⁑ᶯ˄᧒䪸 1 ઼᧒ 䪸 4˅ਸˈ䲔~4.3 kb ⲴⓀᙗᵲӔᶑᑖཆˈ䘈ӗ⭏ҶⓀҾ䍘㋂ PV-ZMAP1043 Ⲵ~1.2 kb ઼~0.7 kb 亴ᵏབྷሿᶑᑖ˄മ 20ˈ⌣䚃 1 ઼ 2˅DŽᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ⭘ Nsi I ⎸ॆਾо亴ݸ⭘ Sph I ⎸ॆⲴ䍘㋂䖭փ PV-ZMAP1043 ਸˈ䲔~4.3 kb ⲴⓀᙗᵲӔᶑᑖཆˈ䘈൘~7.1 kb ઼~1.8 kb ༴ӗ⭏ 2 ᶑ亴ᵏབྷ ሿⲴᶑᑖ˄മ 20ˈ⌣ 䚃 3˅DŽ䱣ᙗሩ➗ⲴỰࠪ㺘᰾᧒䪸оަ䶦ḷᒿࡇਟԕᵲӔDŽ Ӿ LH198 BC3F3ǃLH198 BC3F4ǃLH198 BC3F6ǃLH198 BC3F7 ઼˄LH198 BC3F7 × LH287˅F1 цԓᨀਆⲴ DNA ⭘ Nsi I ⎸ॆ˄മ 20ˈ⌣䚃 5ǃ6ǃ7ǃ8 ઼ 10˅ˈ䲔к䘠ⓀᙗᵲӔᶑᑖཆˈ䘈൘~6.4 kbǃ~2.0 kb ઼~1.7 kb ༴ӗ⭏ 3 ᶑ亴ᵏᶑᑖ˄㺘 7˅DŽ~1.7 kb ᶑᑖԓ㺘Ҷᨂޕ T-DNA Ⲵ 5’ᵛㄟԕ৺аሿ䜘࠶ 㘬สഐ㓴 DNAˈㅖਸ~1.7 kb 䗩⭼⡷⇥Ⲵ亴ᵏབྷሿ˗~2.0 kb ᶑᑖਜ਼ᴹח䛫᧕Ⲵ а䜘࠶ᨂޕ DNA 䜘ᒿࡇDŽ~6.4 kb ᶑᑖԓ㺘 T-DNA Ⲵ 3’ᵛㄟԕ৺ᨂޕ 3’ㄟ 㘬䛫᧕Ⲵสഐ㓴 DNAˈㅖਸ @ \ 䗩⭼⡷⇥Ⲵ亴ᵏབྷሿDŽMON 87427 Ⲵח LH198 BC3F3ǃLH198 BC3F6ǃLH198 BC3F7 ઼˄LH198 BC3F7 × LH287˅F1 цԓ˄മ 20ˈ⌣䚃 5ǃ6ǃ7ǃ8 ઼ 10˅ӗ⭏Ⲵᑖ䉡⢩ᖱо LH198 BC3F4 цԓ ਼˄മ 14ˈ⌣䚃 4 ઼ 11˅ˈ㺘᰾ MON 87427 ѝঅњᤧ䍍Ⲵ T-DNA ൘ᡰ䘹ц ԓѝਟԕっᇊ䚇ՐDŽ 68 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ20. MON 87427ᨂޕᒿࡇ൘ཊцԓ䰤っᇊⲴSouthern࠶᷀˖᧒䪸1઼᧒䪸4 ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢DŽ᧒䪸 1 ઼᧒䪸 4 ӵ㾶ⴆ䜘࠶ T-DNA˄㿱 ᵜ⭣䈧ҖⲴമ 6 ઼മ 13˅DŽLH198 BC3F3ǃLH198 BC3F6 ઼ LH198 BC3F7 Ⲵ DNA ᱟӾਦ⡷ᨀਆˈަᆳцԓ DNA ᱟӾᆀѝᨀਆⲴDŽ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ ࠷䞦⎸ॆⲴ DNA ṧ૱DŽ⌣䚃䈤᰾ྲл˄ᤜਧ㺘⽪⭘Ҿ⎸ॆⲴ࠷䞦˅˖ ⌣䚃 ṧ૱᧿䘠 [᧒䪸 1 ઼᧒䪸 4[ᖃҾ~0.1 ؽสഐ㓴 DNAޕሩ➗ LH198 × HiII (Nsi I) 1 [᧒䪸 1 ઼᧒䪸 4[ᖃҾ~1.0 ؽสഐ㓴 DNAޕሩ➗ LH198 × HiII (Nsi I) 2 [䞦࠷䍘㋂ (Sph I) [ᖃҾ~1.0 ؽสഐ㓴 DNAޕሩ➗ LH198 × HiII (Nsi I) 3 4 ሩ➗ LH198 × HiII (Nsi I) 5 MON 87427 (LH198 BC3F3) (Nsi I) 6 MON 87427 (LH198 BC3F4) (Nsi I) 7 MON 87427 (LH198 BC3F6) (Nsi I) 8 MON 87427 (LH198 BC3F7) (Nsi I) 9 ሩ➗ LH198 × LH287 (Nsi I) 10 MON 87427([LH198 BC3F7 × LH287] F1) (Nsi I) ㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠 69 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2˅MON 87427 ሬޕᙗ⣦Ⲵ䚇Ր࠶ ൘ MON 87427 Ⲵ⹄ウᔰਁ䗷〻ѝˈ䘋㹼ҶޣⲴ䚇Ր࠶䈅傼ˈԕ䇴ՠ MON 87427 ѝᨂޕⲴ cp4 epsps สഐ㺘䗮ⴂⲴ䚇ՐっᇊᙗDŽ࡙⭘ᯩ⍻傼ᶕ∄䖳 ᇎ䱵࠶∄઼ֻᦞᆏᗧቄ䚇Ր㿴ᖻ䇑㇇Ⲵ亴ᵏ࠶∄ֻⲴᤏਸ〻ᓖDŽ ᯩⲴ䇑㇇ޜᔿѪ˖ 02 ` 5 |} M ަѝ˖o= ᇎ䱵㿲ሏࡠⲴสഐරᡆ㺘⧠ර仁⦷˗e= ⨶䇪亴ᵏสഐරᡆ㺘⧠ර仁⦷˗ 㔏䇑ᱮ㪇≤ᒣ䇮ᇊѪ ` DŽ 䖜ॆᗇࡠⲴ R0 ԓἽṚ⭘ LH198 എӔࠐԓਟԕᗇࡠᵲਸⲴ LH198 BC3F1 ц ԓDŽሶ LH198 BC3F1 㠚Ӕਟԕᗇࡠ࠶㗔փ LH198 BC3F2DŽӾ LH198 BC3F2 ѝ䘹ᤙаṚᣇ㥹⭈㟖ἽṚ䘋а↕㠚Ӕਟԕᗇࡠ LH198 BC3F3 ˈ䘹ᤙ LH198 BC3F3 ѝ㓟ਸփ㠚Ӕᗇࡠ LH198 BC3F4˄മ 18˅DŽLH198 BC3F4 Ⲵᆀ ⭘ҾഎӔ䖜㛢ᶕ䘋а↕ᔰਁ୶ъॆⲴ૱DŽާփᱟ⭘нਜ਼ cp4 epsps สഐ㺘䗮ⴂ ⲴӢᵜ˄〠Ѫ䖞എӢᵜˈRP˅എӔDŽഎӔᖃԓѪᵲਸ㗔փˈ〠Ѫ˄RP × LH198 BC3F4˅ BC0F1DŽ䘋а↕എӔᗇࡠ˄RP × LH198 BC3F4˅BC1F1ˈሩަ௧ᯭ㥹⭈㟖ਟԕ䘹 ᤙᣇ㥹⭈㟖ⲴἽṚDŽ⭘ᣇᙗἽṚഎӔᗇࡠ BC2F1ˈ൘ BC2F1 цԓ䘹ᤙᣇᙗἽ Ṛ㠚Ӕᗇࡠ BC2F2 ԓDŽ൘ BC1F1ǃBC2F1 ઼ BC2F2 цԓ䈳ḕ㥹⭈㟖ᣇᙗἽṚⲴ ࠶∄ֻˈBC1F1 ઼ BC2F1 ⲴᣇᙗἽṚ:ᝏἽṚⲴ⨶䇪࠶∄ֻѪ 1:1ˈ㘼 BC2F2 Ⲵ⨶䇪࠶∄ֻѪ 3:1˄മ 18˅DŽ䈳ḕⲴἽṚᮠǃ⨶䇪࠶∄ֻ৺ަᯩ ⍻傼㔃᷌㿱㺘 11DŽӾ䘉Ӌ㔃᷌ਟⴻࠪˈMON 87427 Ⲵᣇ㥹⭈㟖ᙗ⣦ㅖਸᆏᗧቄ অสഐ䚇Ր㿴ᖻDŽӾ㘼䇱ᇎ MON 87427 ѝⲴ cp4 epsps สഐ㺘䗮ⴂ൘অаս⛩к ӵᴹঅᤧ䍍ᨂޕˈᒦਟ൘ਾԓѝっᇊ䚇ՐDŽ ֻ∄㺘11. MON 87427Ⲵ㓴㓷⢩ᔲᙗ㥹⭈㟖ᣇᙗⲴ䚇Ր࠶ 㿲ሏἽṚ 㿲ሏࡠ 㿲ሏࡠ 亴ᵏⲴ 亴ᵏⲴ цԓ 1 2 ᱮ㪇ᙗ ᙫᮠ 2 䱣ᙗṚᮠ 䱤ᙗṚᮠ 䱣ᙗṚᮠ 䱤ᙗṚᮠ BC1F1 238 109 129 119 119 1.6807 >0.05 BC2F1 290 145 145 145 145 0 >0.05 BC2F2 1107 820 287 830 277 0.5062 >0.05 1 ᡰ䘹⭘цԓᶀᯉⲴ䉡㌫ޣ㌫䈧৲㿱മ 18˗ 2 䇴ԧⲴἽṚᙫᮠˈަѝ䱣ᙗṚѪ㥹⭈㟖㙀ਇරἽṚˈ䱤ᙗṚѪ㥹⭈㟖ᝏරἽṚDŽ 3˅MON 87427 н਼цԓᶀᯉ CP4 EPSPS 㺘䗮Ⲵ Western ࠶᷀ ࡽ 2.6.3 䜘࠶ᡰ䘠ˈ֯⭘ Western ᯩ⌅࠶᷀ MON 87427 㛢䉡㌫Ⲵ 5 њцྲ ԓᶀᯉˈवᤜ˖LH198 BC3F3 цԓⲴਦ⡷઼ LH198 BC3F4ǃLH198 BC3F6ǃ LH198 BC3F7 ઼˄LH198 BC3F7 × LH287˅F1 цԓⲴᆀ˗ሩ➗ᶀᯉѪਇփ LH198 × HiII ⲴᆀDŽ࠶᷀㔃᷌㺘᰾ CP4 EPSPS 㳻ⲭ൘ MON 87427 㛢䉡㌫Ⲵ 5 њцԓᶀᯉѝ൷ਟԕっᇊ㺘䗮˄മ 19˅DŽ 70 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.2 䖢ะഖὃ⢟ф։ᡌӨᵢὃ⢟൞⧥ູᆿޞᙝᯯ䶘Ⲻᐤᔸ 3.2.1 ⭕⇌ᯯᕅૂ⭕⇌⦽ ൘ 2008 ᒤ㖾ഭ 16 њൠ⛩Ⲵ⭠䰤䈅傼ѝ䈳ḕҶ 14 њоἽṚ⭏䮯઼ਁ㛢ᴹ ޣⲴᙗ⣦ˈ䘉Ӌ䈅傼ൠ⛩สᵜਟԕ㾶ⴆ㖾ഭⲴѫ㾱⦹㊣ӗ४Ⲵ⭏䮯ᶑԦDŽ ᙫҾ㺘 12DŽ≶MON 87427 ઼ሩ➗Ⲵ 14 њᙗ⣦൘ 16 њ䈅傼ൠ⛩Ⲵᒣ൷٬ 㺘12. 2008ᒤ㖾ഭ⭠䰤䈅傼ѝMON 87427৺ަሩ➗Ⲵ㺘⧠රᙗ⣦Ⲵᒣ൷٬ S.E.˅ ৲㘳૱ᮠ٬㤳ത1˄ᒣ൷٬ 㺘⧠රᙗ⣦˄অս˅ ᴰབྷ٬ MON 87427 ሩ➗ ᴰሿ٬ ᒬ㤇⭏⍫࣋˄1-9㓗˅ 2.7 (0.19)* 2.4 (0.17) 1.0 5.0 ᰙᵏᒬ㤇ᮠ˄Ṛ/ሿ४˅ 67.7 (1.69) 70.3 (2.01) 55.7 80.3 50%ἽṚᮓ㊹ཙᮠ˄d˅ 63.9 (1.15) 63.5 (1.19) 45.7 78.0 50%ἽṚᣭэཙᮠ˄d˅ 62.9 (1.07) 62.7 (1.09) 46.7 75.0 㔯ᙗ˄1-9㓗˅ 5.9 (0.34) 5.6 (0.35) 2.0 9.0؍ ょս儈ᓖ˄in.˅ 42.0 (0.89) 41.9 (0.97) 26.3 56.1 Ṛ儈˄in.˅ 91.4 (1.50) 90.8 (1.50) 73.9 103.1 лょᮠ˄ょ/ሿ४˅ 0.6 (0.22) 0.5 (0.17) 0.0 2.7 ԿṚᮠ˄Ṛ/ሿ४˅ 7.7 (2.58) 5.5 (1.77) 0.0 71.3ق㤾 ԿṚᮠ˄Ṛ/ሿ४˅ 7.5 (2.49) 5.3 (2.08) 0.0 25.9قṩ ᴰ㓸ἽṚᮠ˄Ṛ/ሿ४˅ 60.8 (0.89) 60.4 (0.88) 54.7 65.8 ㊭㋂ਜ਼≤䟿˄%˅ 19.6 (0.55) 20.3 (0.62) 16.0 27.4 ᇩ䟽˄lbs/bu˅ 55.3 (0.33) 55.2 (0.38) 51.6 58.6 ᣈ㇇ӗ䟿˄bu/a˅ 156.9 (5.47) 165.4 (6.71) 94.6 193.8 ⌘˖䈅傼䇮䇑䟷⭘ᆼޘ䲿ᵪ४㓴䇮䇑ˈ3⅑䟽༽ˈS.E. = ḷ߶ᐞˈn=48DŽ * 㺘᰾⍻䈅૱઼ሩ➗૱䰤ᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲ˄ ` ˅DŽ 㘳૱Ⲵᮠ٬㤳തᱟᦞᐢ㓿୶ъॆⲴ 38 њ૱Ⲵᴰሿ઼ᴰབྷ٬ᗇࠪⲴDŽ৲ 1 MON 87427 ઼䶎䖜สഐሩ➗䰤൘䲔Ҷˈṩᦞᡰᴹ䈅傼ൠ⛩Ⲵᙗ⣦ᒣ൷٬ ᒬ㤇⭏⍫࣋ཆⲴᡰᴹަᆳᙗ⣦䰤⋑ᴹ㔏䇑ᆖᱮ㪇ᙗᐞᔲDŽMON 87427 ઼ሩ➗䰤 ୟаᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲⲴᙗ⣦ᱟᒬ㤇⭏⍫࣋DŽMON 87427 Ⲵᒬ㤇⭏⍫࣋㓗࡛ ∄ሩ➗儈˄2.7 㓗 vs. 2.4 㓗ˈԕ 1-9 ࠶㓗˅DŽቭ㇑Ҽ㘵䰤ᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲˈ նᒣ൷ᮠⲴᐞᔲᖸሿˈӵѪ 0.3˄ޡ 9 㓗˅DŽᒦф MON 87427 Ⲵᒬ㤇⭏⍫࣋㓗 ࡛൘а਼ἽⲴ୶ъॆ৲➗૱Ⲵ㠚❦ਈᔲ㤳ത˄ԕ 1-9 ࠶㓗˅DŽഐ↔ˈ䈕㔏 䇑ᆖкⲴᱮ㪇ᐞᔲнާᴹ⭏⢙ᆖѹDŽ ൘о⭏⇆ᯩᔿ઼⭏⇆⦷ޣⲴᙗ⣦ѝˈྲᮓ㊹઼ᣭэཙᮠǃょս儈ᓖǃṚ Կ઼ӗ䟿ㅹˈMON 87427 оᑨ㿴ሩ➗⋑ᴹᱮ㪇ᙗᐞᔲDŽق儈ǃ 2014 ᒤ൘ਹ᷇ⴱߌ、䲒䘋㹼Ⲵ MON 87427 ᣇ㥹⭈㟖⦹㊣⧟ຳᆹޘᙗ䇴ԧ Ⲵ⭠䰤䈳ḕᮠᦞҏ㺘᰾ˈ൘ṭษൠ䈅傼ᶑԦлˈ䖜สഐ⦹㊣ MON87427 ൘Ṛ儈ǃ 71 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㾶ⴆᓖǃӗ䟿ǃਾԓᆀਁ㣭⦷ㅹᯩ䶒ˈоሩᓄⲴ䶎䖜สഐ⦹㊣ᰐᱮ㪇ᐞᔲDŽ 㔬кᡰ䘠ˈMON 87427 Ⲵ㺘⧠රᙗ⣦˄वᤜо⭏⇆ᯩᔿ઼⭏⇆⦷ޣⲴ ᙗ⣦˅оሩ➗⋑ᴹ⭏⢙ᆖѹкⲴᐞᔲˈнՊ໎࣐ަ䖜ਈѪᴹᇣ⭏⢙ᡆሩ⧟ຳ DŽੁٮӗ⭏н࡙ᖡ૽Ⲵ 3.2.2 ՖᯯᕅૂՖ㜳࣑ ⦹㊣Ҿ䳼䳴਼Ṛᔲ㣡ᦸ㊹⢙DŽ᰾⺞ MON 87427 ৺ަᑨ㿴ሩ➗Ⲵ㣡㊹ ᖒᘱ઼㣡㊹⭏⍫࣋ᱟᆈ൘ᐞᔲਟԕ䇴ԧҼ㘵൘Րᯩᔿ઼Ր㜭࣋ᯩ䶒ᱟ ᆈ൘ᐞᔲDŽ⭘Ҿ⹄ウⲴ㣡㊹ᱟӾսҾ㖾ഭᇶ㣿䟼ᐎⲴ⭠䰤䈅傼ѝᵚ㓿㥹⭈㟖༴ ⨶Ⲵ MON 87427ǃᑨ㿴ሩ➗ԕ৺ 4 њ୶ъॆⲴ৲➗૱к䟷䳶ⲴDŽ⭠䰤䈅傼Ѫ ᆼޘ䲿ᵪ४㓴ᧂࡇˈ3 ⅑䟽༽ˈ⇿ሿ४䲿ᵪ䘹ᤙ 3 Ṛ⭘Ҿ䟷䳶㣡㊹DŽ㣡㊹⭘ Alexander Ⲵᯩ⌅ḃ㢢˄Alexander, 1980˅DŽ㣡㊹⍫࣋⹄ウ㠣ቁ⇿њṧᵜ䈳ḕ 100 ㋂㣡㊹ˈ㘼㣡㊹ⴤᖴ⹄ウࡉ൘⇿䟽༽⍻䟿 10 ㋂ާᴹԓ㺘ᙗᒦᴹ⍫࣋Ⲵ㣡㊹DŽ ᇎ傼㔃᷌㺘᰾ˈMON 87427 ઼ሩ➗൘㣡㊹㋂ⴤᖴᯩ䶒⋑ᴹ㔏䇑ᆖᱮ㪇ᙗ ᐞᔲ˄㺘 13˅DŽṩᦞⴤ㿲ⴞ⍻ˈMON 87427 ઼ሩ➗Ⲵ㣡㊹ᖒᘱ⋑ᴹ᰾ᱮн਼DŽ ն MON 87427 㣡㊹⭏⍫࣋˄99.7%˅к൘㔏䇑ᆖкᱮ㪇儈Ҿሩ➗˄98.9%˅DŽቭ ㇑ MON 87427 㣡㊹⍫࣋〽ᗞ儈Ҿ୶ъॆ৲➗૱Ⲵᮠ٬㤳തˈн䇪ᱟ MON 87427 䘈ᱟሩ➗ˈަ㣡㊹⍫࣋Ⲯ࠶ᮠ䜭䶎ᑨ儈ˈᒦфҼ㘵ⲴⲮ࠶ᮠѻᐞᴹ 䶎ᑨሿ˄ਚᴹ 0.8%˅DŽ䘉ӋᖸሿⲴᐞᔲнཚਟ㜭ާᴹ⭏⢙ᆖѹDŽഐ↔ˈоᑨ 㿴ሩ➗∄ˈMON 87427 Ⲵ㣡㊹⍫࣋⋑ᴹਁ⭏ᇎ䍘ᙗ᭩ਈˈнՊ໎࣐ ӗ⭏ޘҏнՊሩ⧟ຳᆹˈੁٮMON 87427 Ⲵᵲ㥹ॆᡆ㘵䖜ਈѪަᆳᴹᇣ⭏⢙Ⲵ н࡙ᖡ૽DŽ 㺘13. MON 87427৺ሩ➗㣡㊹㋂⢩ᖱ⢩ᙗ ᒣ൷ᮠ (S.E.) ৲➗૱ᮠ٬㤳ത 1 ⢩ᖱ⢩ᙗ ᴰབྷ٬ MON 87427 ሩ➗ ᴰሿ٬ 㣡㊹⍫࣋ (%) 99.7* (0.0) 98.9 (0.3) 99.2 99.6 㣡㊹ⴤᖴ } 89.9 (1.2) 90.8 (1.6) 87.2 91.5 ⌘˖䈅傼Ѫᆼޘ䲿ᵪ४㓴䇮䇑ˈ3 ⅑䟽༽˗S.E. = ḷ߶ᐞ;n=12DŽ * 㺘᰾൘ MON 87427 ઼ሩ➗䰤ᆈ൘㔏䇑ᆖᱮ㪇ᙗᐞᔲ˄ ` ˅DŽ ᇊⲴDŽ⺞➗૱ᮠ٬㤳തᱟᦞ 4 њᐢ㓿୶ъॆἽⲴ৲➗૱Ⲵᴰሿ٬઼ᴰབྷ٬৲ 1 72 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.2.3 Շⵖᵕ ᆀⲴ㨼ਁ઼Ձⵐᵪࡦ൘н਼⢙ѻ䰤ᆈ൘䖳བྷਈॆˈަ䚇Րสҏ䶎ᑨ༽ ᵲDŽնᆀՁⵐ⢩ᙗ˄ྲ⺜ᇎᆀ˅৸ᱟᵲ㥹Ⲵ䟽㾱⢩ᖱ˄Anderson, 1996; Lingenfelter and Hartwig, 2003˅DŽ䟷⭘ḷ߶ᯩ⌅ᶕ䇴ԧἽ⢙ᆀⲴ㨼ਁ઼Ձⵐ⢩ ᙗ䶎ᑨ䟽㾱DŽᇈᯩᆀ࠶᷀ᇦॿՊ˄AOSA˅ᱟഭ䱵кᲞ䙽䇔ਟⲴᆀ࠶᷀⍻䈅 ᵪᶴDŽAOSA ᧘㦀 20/30°C ᱟ࠶᷀⦹㊣ᆀ㨼ਁⲴ⨶ᜣᓖ˄AOSA, 2007˅DŽ ᵜ⹄ウ䲔Ҷ䟷⭘ AOSA ᧘㦀Ⲵᓖ˄20/30°C˅ཆˈ䘈䟷⭘ 5°Cǃ10°Cǃ20°C ઼ 30°C ᚂԕ৺ 10/20°C ઼ 10/30°C ਈ༴⨶ᶕ⍻ᇊ MON 87427ǃሩ➗઼ 7 њ ৲➗૱Ⲵᆀ㨼ਁ⦷DŽ㻛⍻䈅Ⲵᆀᱟ 2008 ᒤ൘㖾ഭ⡡㦧ॾᐎ઼Ժ࡙䈪Ժᐎ Ⲵ⭠䰤䈅傼᭦㧧ⲴDŽᇎ傼㔃᷌≷ᙫҾ㺘 15DŽ ṩᦞ 2 њ䈅傼ൠ⛩Ⲵᒣ൷ᮠˈMON 87427 ઼ሩ➗൘ AOSA ᧘㦀ᓖ˄20/30°C˅ ԕ৺ 10°Cǃ30°Cǃ10/20°C ઼ 10/30°C лⲴ⍻䈅㔃᷌⋑ᴹԫօ㔏䇑ᆖᱮ㪇ᐞᔲ˄㺘 15˅DŽ↔ཆˈMON 87427 ઼ሩ➗䰤൘ᡰᴹᓖᶑԦлⲴ⺜ᇎᆀⲮ࠶ᮠᯩ䶒䜭н ᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲDŽᆀ㨼ਁ⍻䈅㔃᷌㺘᰾ˈ൘ԕлᓖᶑԦ઼㨼ਁ⢩ᖱ⢩ᙗ ᯩ䶒лˈMON 87427 ઼ሩ➗ᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲ˖൘ 5°C ᶑԦлⲴ↫ӑᆀᮠ ˄6.5%ሩ 11.1%˅઼ᴹ⍫࣋੨⏘ᆀᮠ˄93.5%ሩ 88.9%˅˗൘ 20°C ᶑԦлⲴ㨼 ਁᆀᮠ˄98.9%ሩ 97.1%˅઼↫ӑᆀᮠ˄1.1%ሩ 2.9%˅DŽ䘉Ӌᆈ൘㔏䇑ᆖᱮ 㪇ᐞᔲⲴᮠ٬ᐞ䐍䜭ᖸሿˈᒦф൘н਼ᓖᶑԦл㔃᷌ᒦна㠤DŽ↔ཆˈ൘ 5°C ᶑԦл MON 87427 Ⲵ↫ӑᆀ઼ᴹ⍫࣋੨⏘ᆀⲮ࠶ᮠԕ৺൘ 20°C ᶑԦлⲴ 㨼ਁᆀ઼↫ӑᆀⲮ࠶ᮠ൷൘୶ъॆ৲➗૱Ⲵᮠ٬㤳തDŽഐ↔ˈ䘉Ӌ㔏䇑 ᆖᱮ㪇ᐞᔲнާᴹ⭏⢙ᆖѹˈн㺘᰾оሩ➗∄ MON 87427 ൘ᆀՁⵐᡆ㨼 ਁᯩ䶒ਁ⭏Ҷ᭩ਈDŽ 3.2.4 䘸ᓊᙝ 㺘 12 ޣҾߌ㢪ᙗ⣦઼㺘⧠රᙗ⣦Ⲵ⹄ウ㔃᷌㺘᰾ˈMON 87427 ઼ሩ➗൘䘲 Կǃقᓄᙗᯩ䶒⋑ᴹᐞᔲDŽMON 87427 ઼ሩ➗൘ἽṚᮠǃᔰ㣡ᮓ㊹ᰦᵏǃṚ儈ǃ ӗ䟿ㅹᙗ⣦ᯩ䶒⋑ᴹᱮ㪇ᐞᔲDŽMON 87427 ઼ሩ➗䰤ୟаᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲ Ⲵᙗ⣦ᱟᒬ㤇⭏⍫࣋DŽMON 87427 Ⲵᒬ㤇⭏⍫࣋㓗࡛∄ሩ➗վ˄2.7 㓗ሩ 2.4 㓗ˈ ޡ࠶ 1-9 㓗˅DŽቭ㇑Ҽ㘵䰤ᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲˈնᒣ൷ᮠⲴᐞᔲᖸሿˈӵѪ 0.3DŽ ᒦф MON 87427 Ⲵᒬ㤇⭏⍫࣋㓗࡛൘а਼ἽⲴ୶ъॆ৲➗૱Ⲵ㠚❦ਈᔲ㤳 ത˄1-5 㓗˅DŽഐ↔ˈ䈕㔏䇑ᆖкⲴᱮ㪇ᐞᔲнާᴹ⭏⢙ᆖѹDŽ ਇߌъ䜘Ⲵငᢈˈߌъ䜘䖜สഐἽ⢙⧟ຳᆹޘⴁⶓỰ傼⍻䈅ѝᗳ˄䮯᱕˅᳘ ਹ᷇ⴱߌъ、ᆖ䲒Ҿ 2014 ᒤᆼᡀҶሩ MON 87427 ᣇ䲔㥹ࡲ⦹㊣Ⲵ⧟ຳᆹޘᙗ䇴 ՠDŽ㔃᷌㺘᰾ˈ䖜สഐᣇ䲔㥹ࡲ⦹㊣ MON87427 ሩ㠣ቁ 2 ؽ᧘㦀ࡲ䟿௧ᯭⲴߌ 䗮䲔㥹ࡲާᴹ㢟ྭⲴ㙀ਇᙗDŽ൘㦂ൠ઼ṭษൠ䈅傼ᶑԦлˈ䖜สഐ⦹㊣ MON87427 Ⲵ⭏ᆈㄎҹ㜭࣋оަሩᓄⲴ䶎䖜สഐ⦹㊣൷ᰐᱮ㪇ᐞᔲDŽ ⭡↔ਟԕᗇࠪ㔃䇪ˈMON 87427 ઼ሩ➗൘䘲ᓄᙗᯩ䶒⋑ᴹᱮ㪇ᙗᐞᔲDŽ 73 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.2.5 ⭕ᆎㄔҿ㜳࣑ MON 87427 ઼ሩ➗Ⲵߌ㢪ᙗ⣦઼㺘⧠රᙗ⣦⹄ウवᤜҶἽṚ⭏䮯ǃ㑱⇆ǃ Կ઼㩭㋂˅ㅹⲴ䇴ՠˈ㔃᷌˄㺘 12˅㺘᰾ MON 87427 ઼ሩقྲ˄ӗ䟿ǃ㩭㋂ᙗ ➗ѻ䰤൘䘉Ӌᙗ⣦ᯩ䶒⋑ᴹᐞ࡛DŽਖཆˈՐ㜭઼࣋ᯩᔿ˄㺘 13˅઼ᆀ㨼ਁ Ձⵐ⢩ᙗ˄㺘 15˅Ⲵ⹄ウ㔃᷌ҏ㺘᰾ MON 87427 ઼ሩ➗ѻ䰤⋑ᴹᐞᔲDŽ о⧟ຳѝަᆳ⭏⢙Ⲵӂ⭘ޣ㌫ԕ৺⧟ຳ㛱䘛ᯩ䶒Ⲵ䇴ՠҏਟѪ䇴ՠ MON 87427 䖜ਈѪᴹᇣ⭏⢙Ⲵਟ㜭ᙗԕ৺⭏ᆈㄎҹ㜭࣋ⲴᨀᦞDŽ ޣҾ MON 87427 ሩ䶎⭏⢙㛱䘛Ⲵ৽ᓄˈ䇴ԧⲴᇩवᤜ˖վǃᰡǃ⏍ǃ 䵌߫ǃߠ䴩ǃ儈ǃⸯ⢙∂㍐ǃ≞㕪ཡǃ㩕ޫ㕪ཡǃ൏༔ᶯ㔃ǃ൏༔≤価઼৺仾 ㅹDŽ൘ 172 亩⅑Ⲵᮠᦞሩ∄ѝˈMON 87427 ઼ሩ➗ѻ䰤Ⲵ㿲ሏ㓗࡛൷ᰐᐞᔲ˄㺘 ˅DŽޣҾ MON 87427 ሩ⯵ᇣ㛱䘛Ⲵ৽ᓄˈ䇴ԧⲴᇩवᤜ⛝⯭⯵ǃ㓶㧼ᙗਦ ᷟ⯵ǃ㓶㧼ᙗਦᯁ⯵ǃ唁✏➔⯵ǃ㽀ᯁ⯵ǃょ㞀⯵ǃᯁ⯵ǃ䮠ᆒ㧼ǃ⚠ᯁ⯵ǃ ㊭㋂㓒㢢ᶑ㓩⯵ǃਦᷟ⯵ǃ⦹㊣⸞㣡ਦ⯵∂ǃབྷᯁ⯵ǃ㍛㤇㔬ਸ⯷ǃ㞀䴹⯵ǃ㓩 ᷟ⯵ǃṩ㞀⯵ǃ䬸⯵ǃ㤇ᷟ⯵ǃ㤇㞀⯵ǃ唁ょ⯵ǃሿᯁ⯵ǃ㤾㞀⯵઼⦹㊣㓶㧼ᙗ ᷟ㨾⯵ㅹ⯵ᇣDŽ൘ᙫޡ 210 亩⅑Ⲵ∄䖳ѝˈMON 87427 ઼ሩ➗ѻ䰤Ⲵ㿲ሏ㓗࡛ ൷ᰐᐞᔲ˄㺘 16˅DŽ 2014 ᒤߌъ䜘䖜สഐἽ⢙⧟ຳᆹޘⴁⶓỰ傼⍻䈅ѝᗳ˄䮯᱕˅ሩ MON 87427 Ⲵ⭏ᆈㄎҹ㜭࣋䘋㹼Ự⍻㔃᷌㺘᰾˖˄1˅൘㦂ൠ䈅傼ᶑԦлˈ䖜สഐ⦹㊣ MON 87427 Ⲵ⭏ᆈㄎҹ㜭࣋ᱮ㪇ᕡҾᵲ㥹ˈфоަሩᓄⲴ䶎䖜สഐ⦹㊣ᰐᱮ㪇 ᐞᔲ˗˄2˅൘ṭษൠ䈅傼ᶑԦлˈ䖜สഐ⦹㊣ MON87427 ൘Ṛ儈ǃ㾶ⴆᓖǃӗ 䟿ǃਾԓᆀਁ㣭⦷ㅹᯩ䶒ˈоሩᓄⲴ䶎䖜สഐ⦹㊣ᰐᱮ㪇ᐞᔲDŽ ٮ⢙㔬ਸк䘠㔃᷌ˈMON 87427 ઼ሩ➗൘⭏ᆈㄎҹ㜭࣋ԕ৺䖜ਈѪᴹᇣ⭏ ੁᯩ䶒⋑ᴹᐞ࡛DŽ 74 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘14. 2008ᒤ㖾ഭ⭠䰤䈅傼ѝMON 87427઼ᑨ㿴ሩ➗ሩ䶎⭏⢙㛱䘛৽ᓄ㓗࡛Ⲵ ሩ∄㔃᷌≷ᙫ 䶎⭏⢙㛱䘛 ᡰᴹ䈅傼⛩㿲ሏሩ∄亩 MON 87427оሩ➗䰤ᰐᐞᔲⲴ ⅑ᮠ1 㿲ሏሩ∄亩⅑ᮠ ਸ䇑 172 172 վ 66 ᒢᰡ 13 13 ⍚⏍ 19 19 䵌߫ 44 ߠ䴩 26 26 儈 25 25 ⸯ⢙∂㍐ 11 ≞㕪ཡ 22 㩕ޫ㕪ཡ 13 13 ൏༔ᶯ㔃 77 ≤価઼൏༔2 77 仾 49 49 ⌘˖䈅傼䟷⭘ᆼޘ䲿ᵪ४㓴䇮䇑ˈ3⅑䟽༽DŽ䈳ḕᮠᦞ䰤ᱟᆈ൘ᐞᔲᱟสҾ㿲ሏ㓗࡛ ᶕࡔᯝˈ㘼䶎ṩᦞ㔏䇑࠶᷀DŽ൘⦹㊣⭏䮯Ⲵ4њ䱦⇥䘋㹼䈳ḕ˖V2~V4ǃV10~V15ǃ VT~R3ǃR6DŽ 1 䈅傼ൠ⛩वᤜ˖IA1 = Wapello Co., IA; IA2 = Benton Co., IA; IL1 = Stark Co., IL; IL2 = Clinton Co., IL; IN1 = Montgomery Co., IN; IN2 = Boone Co., IN; MI = Ottawa Co., MI; MO = Butler Co., MO; OK = Caddo Co., OK; PA = Berks Co., PA; TX = Armstrong Co., TX; WI = Walworth Co., WI; ARNE = Jackson Co., AR; IARL = Jefferson Co., IA; ILWY = Stark Co., IL; INRC = Parke Co., IN. 2 वᤜ╛♼઼≤価઼൏༔ 75 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘15. MON 87427઼ሩ➗ᆀⲴ㨼ਁ⢩ᖱ⢩ᙗ⍻ᇊ㔃᷌ ᓖ༴⨶ ᒣ൷ᮠ %(S.E.)1 ৲➗૱ᮠ٬㤳ത 2 㨼ਁ⢩ᖱ⢩ᙗ (°C) MON 87427 ሩ➗ Min Max 5 㨼ਁᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ᴹ⍫࣋⺜ᇎᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ↫ӑᆀ 6.5* (1.5) 11.1 (2.5) 3.8 6.8 ᴹ⍫࣋੨⏘ᆀ 93.5* (1.5) 88.9 (2.5) 93.3 96.3 10 㨼ਁᆀ 82.4 (2.4) 79.0 (1.8) 87.3 98.3 ᴹ⍫࣋⺜ᇎᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ↫ӑᆀ 3.9 (0.8) 4.4 (0.6) 1.0 5.3 ᴹ⍫࣋੨⏘ᆀ 13.8 (1.9) 16.6 (1.8) 0.0 11.3 20 㨼ਁᆀ 98.9* (0.3) 97.1 (0.6) 95.8 99.3 ᴹ⍫࣋⺜ᇎᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ↫ӑᆀ 1.1* (0.3) 2.9 (0.6) 0.8 4.3 ᴹ⍫࣋੨⏘ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 30 㨼ਁᆀ 97.4 (0.5) 98.4 (0.5) 97.0 99.8 ᴹ⍫࣋⺜ᇎᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ↫ӑᆀ 2.6 (0.5) 1.6 (0.5) 0.3 3.0 ᴹ⍫࣋੨⏘ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 10/20 㨼ਁᆀ 98.0 (0.5) 98.4 (0.6) 95.8 99.8 ᴹ⍫࣋⺜ᇎᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ↫ӑᆀ 2.0 (0.5) 1.6 (0.6) 0.3 4.3 ᴹ⍫࣋੨⏘ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 10/30 㨼ਁᆀ 98.5 (0.3) 97.9 (0.6) 97.0 99.5 ᴹ⍫࣋⺜ᇎᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ↫ӑᆀ 1.5 (0.3) 2.1 (0.6) 0.5 3.0 ᴹ⍫࣋੨⏘ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 20/30 ↓ᑨ㨼ਁᆀ 94.4 (0.9) 94.9 (0.9) 93.8 98.8 (AOSA ᧘㦀) 䶎↓ᑨ㨼ਁᆀ 3.8 (1.2) 3.6 (1.1) 0.8 3.8 ᴹ⍫࣋⺜ᇎᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ↫ӑᆀ 1.9 (0.5) 1.5 (0.3) 0.3 4.0 ᴹ⍫࣋੨⏘ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ⌘˖2 њ䈅傼ൠ⛩ˈ䈅傼䇮䇑Ѫ㻲४䇮䇑ˈ4 ⅑䟽༽ˈn=8DŽ * 㺘᰾൘ MON 87427 ઼ሩ➗䰤ᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲ˄ ` ˅DŽ † ⭡Ҿ㕪ቁᮠᦞ㘼ᰐ⌅䘋㹼㔏䇑ᆖ∄䖳DŽ 1 ൘ḀӋᛵߥлˈ⭡Ҿ㔏䇑ᒣ൷ᮠᰦ䟷⭘ 4 㠽 5 ޕˈ㊫ᆀⲴⲮ࠶ᮠѻ઼ਟ㜭нㅹ Ҿ 100%ˈS.E. = ḷ߶ᐞDŽ ᇊⲴDŽ⺞➗૱ᮠ٬㤳തᱟᦞ 4 њᐢ㓿୶ъॆἽⲴ৲➗૱Ⲵᴰሿ٬઼ᴰབྷ٬৲ 2 76 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘16. 2008ᒤ㖾ഭ⭠䰤䈅傼ѝMON 87427઼ᑨ㿴ሩ➗ሩ⯵ᇣ৽ᓄ㓗࡛Ⲵሩ∄㔃᷌ ⯵ᇣ㛱䘛 ᡰᴹ䈅傼⛩㿲ሏሩ∄ MON 87427оሩ➗䰤ᰐᐞᔲ 亩⅑ᮠ1 Ⲵ㿲ሏሩ∄亩⅑ᮠ ਸ䇑 210 210 ⛝⯭⯵ 12 12 㓶㧼ᙗਦᷟ⯵ 11 㓶㧼ᙗਦᯁ⯵ 11 唁✏➔⯵ 11 㽀ᯁ⯵ 11 ょ㞀⯵2 17 17 ᯁ⯵ 88 䮠㜎㧼˄Fusarium˅ 11 11 ⚠ᯁ⯵3 32 32 ㊭㋂㓒㢢ᶑ㓩⯵ 22 ਦᷟ⯵ 55 ⦹㊣⸞㣡ਦ⯵∂⯵ 11 བྷᯁ⯵ 17 17 ㍛㤇㔬ਸ⯷ 11 㞀䴹⯵˄Pythium4˅ 99 㓩ᷟ⯵˄Rhizoctonia˅ 22 ṩ㞀⯵ 66 䬸⯵5 33 33 㤇ᷟ⯵ 12 12 㤇㞀⯵ 22 唁ょ⯵ 77 ሿᯁ⯵ 88 㤾㞀⯵2 16 16 ⦹㊣㓶㧼ᙗᷟ㨾⯵ 55 ⌘˖䈅傼䟷⭘ᆼޘ䲿ᵪ४㓴䇮䇑ˈ3⅑䟽༽DŽ䈳ḕᮠᦞ䰤ᱟᆈ൘ᐞᔲᱟสҾ㿲ሏ㓗࡛ ᶕࡔᯝˈ㘼䶎ṩᦞ㔏䇑࠶᷀DŽ൘⦹㊣⭏䮯Ⲵ4њ䱦⇥䘋㹼䈳ḕ˖V2~V4ǃV10~V15ǃ VT~R3ǃR6DŽ 1 䈅傼ൠ⛩वᤜ˖IA1 = Wapello Co., IA; IA2 = Benton Co., IA; IL1 = Stark Co., IL; IL2 = Clinton Co., IL; IN1 = Montgomery Co., IN; IN2 = Boone Co., IN; MI = Ottawa Co., MI; MO = Butler Co., MO; OK = Caddo Co., OK; PA = Berks Co., PA; TX = Armstrong Co., TX; WI = Walworth Co., WI; ARNE = Jackson Co., AR; IARL = Jefferson Co., IA; ILWY = Stark Co., IL; INRC = Parke Co., IN. 2 ᭦㧧ᰦ䇴ՠⲴょ㞀⯵઼㤾㞀⯵वᤜ5Ṛ䶎㌫㔏䘹ᤙⲴ⠶㻲㤾⿶DŽㅜй⅑䈳ḕᰦ ˄VT~R3˅໎࣐Ҷа⅑ょ㞀⯵䈳ḕDŽ 3 वᤜ⚠ਦ䴹⯵˄gray leaf mold˅DŽ 4 䬸⯵वᤜ㽀䬸⯵઼ਦ䬸⯵DŽ 77 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.2.6 䖢ะഖὃ⢟Ⲻ䚍Ֆ⢟䍞ੇެᆹὃ⢟Ƚࣞ⢟ૂᗤ⭕⢟ਇ⭕䖢〱Ⲻ ਥ㜳ᙝ аᰖᮤਸ䘋Ἵ⢙สഐ㓴ˈT-DNA Ⲵ䗩⭼ᒿࡇቡՊђཡDŽ⭡Ҿ㕪ቁ䗩⭼ᒿࡇ ⲴᑞࣙˈᮤਸⲴ DNA 䖜〫ࡠަԆἽ⢙䟼Ⲵਟ㜭ᙗࠐѾ⋑ᴹˈ䲔䶎䙊䗷ᴹᙗᵲӔ ˄Huttner et al., 1992; Bakkeren et al., 1989˅DŽ 㣡㊹ӻሬⲴสഐ┲〫˄䙊ᑨ〠ѪᔲӔᦸ㊹ᡆสഐ⑇ޕ˅ᱟањᡆཊњสഐᮤ ਸࡠਇփสഐ㓴Ⲵ䟽㾱䙄ᖴDŽสഐ⑇ޕ䙊ᑨਇ⭏⢙઼䶎⭏⢙ഐ㍐Ⲵᖡ૽ˈ∄ྲἽ ⭏⢙ᆖ⢩ᖱǃ㣡㊹⭏⢙ᆖ⢩ᖱ઼㣡㊹䟿ǃփ઼ਇփⲴ㣡ᵏ䟽ਐᰦ䰤ǃ㣡㊹⢙ փ઼ਇփⲴӢ઼〻ᓖԕ৺ᓖǃ⒯ᓖ઼ߌ㢪㙅ㅹઘ䗩⧟ຳDŽสഐ⑇ޕᱟа㠚 ❦⧠䊑ˈഐ↔⋑ᴹᗵ㾱䗷ཊޣ⌘สഐ⑇ޕ䗷〻ˈ㘼ᓄ䟽⛩䇴ԧᨂޕⲴཆⓀสഐᱟ ᆹޘԕ৺ਁ⭏สഐ⑇ޕᱟՊ໎࣐䖜ਈѪᴹᇣ⭏⢙Ⲵ▌࣋DŽ ᔲӔᦸ㊹ᱟ⦹㊣Ⲵപᴹ⢩ᙗˈഐ↔൘ṭษ⦹㊣˄Zea mays L.˅ਁ⭏㣡㊹ ӻሬⲴสഐ┲〫ᱟᗵ❦ⲴDŽབྷ䟿Ⲵ⹄ウਁ⧠˖1˅⭡ҾᔲӔᦸ㊹ˈṭษ⦹㊣ Ⲵสഐ┲〫仁⦷ᱟᖃ儈Ⲵ˗2˅ਁ⭏สഐ┲〫ⲴⲮ࠶ᮠ䲿⦹㊣㗔փǃᵲӔᡆ 㠚Ӕ㌫Ⲵн਼㘼н਼˗3˅㣡㊹Ⓚ઼ਇփⲴ䐍䎺䘌ˈ┲〫Ⲵ≤ᒣቡ䎺վ˗4˅⧟ ຳഐ㍐ਟԕᖡ૽สഐ┲〫Ⲵ〻ᓖ˗5˅൘⭠䰤ᶑԦл⦹㊣㣡㊹㋂Ⲵ⭏⍫࣋ᱟᖸ⸝ Ⲵ˗6˅⦹㊣ᮓ㊹Ⲵᰦᵏሩ䖳䮯˗7˅⦹㊣ਟԕ䙊䗷仾Ⴢᡆ㲛ჂՐ㊹DŽ аӋ⹄ウ㺘᰾ˈṭษ⦹㊣Ⲵสഐ┲〫〻ᓖՊṩᦞ䈅傼䇮䇑ǃ⧟ຳᶑԦ઼ Ự⍻ᯩ⌅Ⲵн਼㘼ਈॆDŽᙫⲴᶕ䈤ˈྲ᷌㣡㊹ⓀоਇփⲴ䐍䎵䗷 200 mˈਁ⭏ สഐ┲〫Ⲵਟ㜭ᙗቡՊ䱽ࡠ 1%ԕлDŽ䘉ᯩ䶒ؑሩ൘⦹㊣㛢ǃᆀ⭏ӗǃ ᆈ৺ަᆳᓄ⭘ᰦᴹ᭸ൠ᧗ࡦสഐ┲〫ᱟ䶎ᑨᴹ⭘ⲴDŽ؍ᙗ 㠣Ҿ MON 87427 ੁަᆳ⦹㊣ਁ⭏สഐ┲〫нՊᕅ䎧ᆹޘᯩ䶒Ⲵ亮㲁ˈഐѪ ޘᓧ઼⧟ຳᆹڕབྷ䟿⹄ウᐢ㓿䇱᰾ MON 87427 वਜ਼Ⲵ cp4 epsps สഐнՊሩӪ㊫ ᑖᶕডᇣDŽ ⦹㊣઼аᒤ⭏໘㾯କ㊫㴰哽˄Zea mays subsp. mexicana˅໘㾯କ઼ডൠ傜 ⲴаӋൠ४ྲ᷌Ἵ䐍ᖸ䘁ˈਟਁ⭏㠚❦ӂᦸ㊹ᒦ㺘⧠ᵲӔӢ઼ᙗ˄Wilkes, DŽն Ellstrand ㅹ˄2007˅Ⲵ⹄ウ㺘᰾ˈণ֯ሶ⦹㊣઼໘㾯କ⦹㴰哽൘а˅1972 䎧ˈҼ㘵ѻ䰤ਁ⭏ᔲӔⲴ仁⦷䘈ᱟᖸվⲴDŽྲ᷌㘳㲁ᔰ㣡ᰦᵏǃൠ⨶䐍䳄઼ ਁ㛢〻ᓖㅹഐ㍐ˈҼ㘵нཚਟ㜭ਁ⭏㠚❦ᔲӔDŽ ণ֯䟷⭘⢩↺ᢰᵟˈ⦹㊣о᪙ᬖⲴཊᒤ⭏⢙ⲴᵲӔҏᱟ䶎ᑨഠ䳮ⲴDŽ ,ཆˈণ֯ᦸ㊹ᵲӔᡀ࣏ˈަਾԓҏՊн㛢ᒦф൘䚇ՐкҏᱟнっᇊⲴ˄Galinat↔ 1988; Russell and Hallauer, 1980˅DŽ 㔬кᡰ䘠ˈMON 87427 ѝⴞⲴสഐ䙊䗷ᴹᙗᵲӔ䖜〫Ⲵ㤳തਚ䲀Ҿਟԕ઼ ṭษ⦹㊣ᵲӔӢ઼Ⲵˈа㡜Ѫṭษ⦹㊣ⲴаӋ૱DŽੁަᆳἽ⢙⢙┲ 〫Ⲵਟ㜭ᙗࠐѾнᆈ൘DŽ ྲ਼൘ 1.4.4 䜘࠶᧿䘠Ⲵ䛓ṧˈⴞࡽц⭼к䘈⋑ᴹⴤ᧕䇱ᦞ㺘᰾ᐢ㓿ਁ⭏䗷 Ἵ⢙䚇Րสഐੁࣘ⢙઼ᗞ⭏⢙ѻ䰤Ⲵ≤ᒣ䖜〫˄horizontal transfer˅˄Bertolla and Simonet, 1999˅DŽ 78 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.2.7 䖢ᡆᵸ㥿Ⲻਥ㜳ᙝ ⦹㊣ᱟ儈ᓖ傟ॆ⢙ˈ⋑ᴹӪ䇔Ѫ⦹㊣ቡᱟᵲ㥹ᡆ㘵Պ䖜ਈѪᵲ㥹˄Crockett, 1977; Muenscher, 1980˅ˈ㖾ഭ᭯ᓌҏ⋑ᴹሶ⦹㊣ࡇѪᴹᇣᵲ㥹ⴞᖅѝ˄㿱⌅㿴 7CFR§360˅DŽ↔ཆˈ⦹㊣ᐢ㓿൘ޘ⨳ᒯ⌋Ἵˈ㠣Ӻ⋑ᴹԫօᣕ䇔Ѫ⦹㊣ Ҿᵲ㥹DŽ㓿䗷䮯ᰦ䰤Ⲵ䘹ᤙ઼傟ॆˈ⦹㊣нާ༷ᵲ㥹Ⲵสᵜ⢩ᖱ˄ྲᆀՁⵐ⢩ ᙗ઼ᢙᮓᵪࡦ˅ˈᔰӪᐕṭษ⧟ຳሶн㜭⭏ᆈ˄Galinat, 1988; Keeler, 1989˅DŽ⦹ ㊣᷌ょ⭡㤎ਦव㼩ˈঅњ㊭㋂ᖸ䳮൘㠚❦ՐDŽণ֯⦹㊣㊭㋂൘䘀䗃䗷〻ѝਟ㜭 Պਁ⭏䚇᫂ˈն⦹㊣ᖸ䳮൘⋏䗩઼䐟䗩ᖒᡀ㠚⭏㤇ᒦ⭏䮯ᡀ⟏DŽণ֯⦹㊣൘⭠䰤 ਟԕᖒᡀ㠚⭏㤇ˈᖸ䳮䘳㝡䎺ߜǃ⢙䖞ǃᵪỠॆ㙅ԕ৺ॆᆖ㦟ࡲ༴⨶ㅹഐ ㍐Ⲵᖡ૽ˈ䘋㘼൘л㥜⭏䮯ᡀ⟏DŽ ԕк 3.2.1~3.2.5 䜘࠶Ⲵ࠶᷀㺘᰾ˈMON 87427 ઼ᑨ㿴ሩ➗൘⭏⇆㜭࣋ǃ ᆀՁⵐ⢩ᙗǃ䘲ᓄᙗ઼⭏ᆈㄎҹ㜭࣋ㅹᯩ䶒⋑ᴹᱮ㪇ᙗᐞᔲ˗оᑨ㿴ሩ➗∄ˈ MON 87427 нՊ໎࣐ަ䖜ਈѪᵲ㥹Ⲵਟ㜭ᙗDŽ 3.2.8 ᣍ⯻㲡䖢ะഖὃ⢟ሯ䶬ḽ⭕⢟䶔䶬ḽ⭕⢟Ⲻᖧଃθऻᤢሯ⧥ ູѣᴿⴀૂᴿᇩ⭕⢟Ⲻᖧଃ MON 87427 㺘䗮Ⲵ CP4 EPSPS 㳻ⲭ˄5-✟䞷щᔿ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦˅ ᱟἽ⢙઼ᗞ⭏⢙փ㣣俉᯿≘ส䞨ԓ䉒䙄ᖴѝⲴа⼧䞨ส䖜〫䞦ˈнҾᣇ⯵ ᡆᣇ㲛สഐˈഐ↔нᆈ൘䶦ḷᡆ䶎䶦ḷ⭏⢙DŽ ൘ 2008 ᒤ㖾ഭⲴ⭠䰤䈅傼ѝˈ䈳ḕҶ㢲㛒ࣘ⢙ⲴѠᓖˈ䈳ḕⲴ㢲㛒ࣘ⢙व ᤜ˖㳌㲛ǃ⦹㊣ཌ㴮ǃ⦹㊣䐣⭢ǃ㵑㵹ǃ㶇㲛ǃਦ㵹ǃेᯩ⦹㊣伏ṩ㲛ǃ䵢ቮ⭢ǃ ইᯩ⦹㊣伏ṩ㲛ǃ㾯ᯩ⦹㊣伏ṩ㲛ǃ㵈㴋ǃབྷ⭢㲛ǃ㽀㥹㳹ǃ㔯㥹㳹ǃ⬒㲛ǃ བྷරᇴ⭏ᙗ㟌㗵ⴞᰶ㲛ǃሿරᇴ⭏ᙗ㟌㗵ⴞᰶ㲛ǃလ㶭઼ሿ㣡㶭DŽޡᴹ 4 њ䈅傼 ൠ⛩ˈ⇿њൠ⛩࠶ 4 њᰦᵏ䈳ḕDŽ൘ᙫޡ 203 亩⅑䈳ḕⲴᮠᦞሩ∄ѝˈᴹ 193 亩⅑൘ MON 87427 ઼ሩ➗䰤нᆈ൘㔏䇑ᆖᱮ㪇ᙗᐞᔲˈᴹ 12 亩⅑ᆈ൘㔏䇑ᆖᱮ 㪇ᙗᐞᔲ˄㺘 ˅DŽቭ㇑൘ḀӋ䈅傼⛩ⲴḀӋ㢲㛒ࣘ⢙ⲴḀ⅑䈳ḕѝ MON 87427 ઼ሩ➗䰤ᆈ൘㔏䇑ᆖᱮ㪇ᙗᐞᔲˈն䘉Ӌ㔏䇑ᆖᐞᔲ൘਼а㢲㛒ࣘ⢙Ⲵн਼ൠ ⛩઼н਼䈳ḕᰦᵏⲴ㺘⧠ᒦна㠤DŽሶ MON 87427 Ⲵᮠ٬о୶ъॆ৲➗૱Ⲵ ᮠ٬∄ˈ㾱Ѹ MON 87427 Ⲵᮠ٬൘৲➗૱Ⲵᮠ٬㤳തˈ㾱Ѹ MON 87427 Ⲵᮠ٬о৲➗૱Ⲵᮠ٬㤳തᐞ䐍ᖸሿDŽഐ↔ˈਟԕᗇࠪ㔃䇪䇔Ѫ㿲ሏࡠⲴ㔏䇑 ᆖᱮ㪇ᐞᔲнާᴹ⭏⢙ᆖѹˈҏоሬޕⲴཆⓀสഐᰐޣDŽ 2014 ᒤˈߌъ䜘䖜สഐ⧟ຳᆹޘⴁⶓỰ傼⍻䈅ѝᗳ˄䮯᱕˅Ự⍻Ҷ MON 87427 ሩ⭏⢙ཊṧᙗⲴᖡ૽ˈ㔃᷌㺘᰾ˈо䶎䖜สഐ⦹㊣∄ˈ䖜สഐ⦹ ㊣ MON87427 ሩ⭠䰤ѫ㾱㢲㛒ࣘ⢙ᮠ䟿઼㗔㩭㔃ᶴǃѫ㾱匎㗵ⴞᇣ㲛ԕ৺ѫ㾱 ⯵ᇣᰐᱮ㪇ᖡ૽DŽ 79 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘17. 2008ᒤ㖾ഭ⭠䰤䈅傼ѝMON 87427઼ᑨ㿴ሩ➗к㢲㛒ࣘ⢙Ѡᓖ㺘⧠ࠪᱮ㪇 ᙗᐞᔲⲴ㔃᷌≷ᙫ ḷ߶ᐞ˅ ৲➗૱ᮠ٬˄䈅傼 ᒣ൷٬ 㢲㛒ࣘ⢙ 䈳ḕᰦᵏ ൠ⛩ MON 87427 ሩ➗ 㤳ത ᴹᇣ㢲㛒ࣘ⢙ ⦹㊣䐣⭢ PA V10~V15 91.7 (26.3)* 59.0 (13.7) 30.3 - 120.3 ⦹㊣䐣⭢ PA R3~R4 12.0 (2.5)* 5.3 (1.5) 3.3 - 10.3 㶇㲛 MO R2 1.3 (0.9)* 0.0 (0.0) 0.0 - 1.0 ਦ㵹 PA V18~VT 7.7 (2.9)* 2.0 (1.2) 2.3 - 10.7 ᴹ⳺㢲㛒ࣘ⢙ 㵈㴋 PA R2 2.3 (0.3)* 0.7 (0.7) 1.0 - 3.7 ⬒㲛 IL1 R1 0.3 (0.3)* 1.3 (0.3) 0.0- 0.3 ⬒㲛 IN2 R1 10.7 (2.8)* 3.0 (1.0) 2.0 - 7.0 བྷරᇴ⭏ᙗ㟌㗵ⴞ IN2 R2 26.7 (3.2)* 9.7 (2.2) 9.3 - 14.7 བྷරᇴ⭏ᙗ㟌㗵ⴞ IN2 R3~R4 47.7 (5.5)* 27.7 (5.3) 29.7 - 36.3 လ㶭 PA V10~V15 0.7 (0.3)* 2.7 (0.7) 1.3 - 4.7 ሿ㣡㶭 IN2 V18~VT 0.3 (0.3)* 3.7 (1.5) 0.3 - 3.3 ሿ㣡㶭 PA R3~R4 14.0 (1.2)* 7.3 (0.7) 8.7 - 17.3 ⌘˖䈅傼䟷⭘ᆼޘ䲿ᵪ४㓴䇮䇑ˈ3⅑䟽༽DŽ൘⦹㊣⭏䮯Ⲵ5њ䱦⇥䘋㹼䈳ḕ˖V10~V15ǃ V18~VTǃR1ǃR2ǃR3~R4DŽ 1 䈅傼ൠ⛩वᤜ˖IL1 = Stark Co., IL; IN2 = Boone Co., IN; MO = Butler Co., MO; PA = Berks Co., PADŽ ሯ⭕ᘷ⧥ູⲺެᆹᴿⴀᡌᴿᇩ֒⭞ 3.2.9 ࡠⴞࡽѪ→ˈ䘈⋑ᴹ䇱ᦞ㺘᰾ MON 87427 ᡆ㘵ަ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭՊ ሩ⭏ᘱ⧟ຳӗ⭏н㢟ᖡ૽DŽCP4 EPSPS ਟԕ൘ MON 87427 Ⲵ㩕ޫ⭏䮯ಘᇈ઼䳼 ᙗ⭏⇆ಘᇈ↓ᑨ㺘䗮ˈն൘䳴ᙗ⭏⇆ಘᇈ˄∑㔂ቲ઼㣡㊹ሿᆒᆀ˅н㺘䗮ᡆ㺘䗮 䟿ᖸվDŽഐ↔൘ᵲӔࡦᰦ௧ᯭ㥹⭈㟖ˈਟԕ֯ MON 87427 Ⲵ㣡㊹䍕㛢ˈ൘ Ḁ〻ᓖк߿ቁަ┲〫Ⲵਟ㜭ᙗDŽ 80 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᓭᖧଃᯯ䶘Ⲻᐤᔸڛ㊱䖢ะഖὃ⢟ф։ᡌӨᵢὃ⢟൞ሯӰ 3.3 ྲࡽᡰ䘠ˈMON 87427 ᱟ䙊䗷ߌᵶ㧼ӻሬ䖜ॆˈሶ PV-ZMAP1043 䍘㋂ѝ Ⲵ T-DNA ሬޕࡠ LH198 × HiII ਇփ૱㌫ѝ㧧ᗇⲴDŽ䈕 T-DNA वਜ਼ᴹањ cp4 epsps สഐ㺘䗮ⴂˈवᤜ e35S ࣘᆀǃhsp70 Ⲵਜ਼ᆀǃCTP2 ਦ㔯փ䖜〫㛭 ᒿࡇǃcp4 epsps 㕆⸱ᒿࡇ઼ nos 㓸→ᆀDŽ 㳻ⲭ䍘⢩ᖱ⢩ᙗ⹄ウ㔃᷌㺘᰾ˈMON 87427 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭоᐢ 㓿൘ѝഭԕ৺ц⭼ަԆഭᇦ㧧ᗇᢩ߶Ⲵཊᣇ㥹⭈㟖⢙ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭᱟ਼ⲴDŽ䘉Ӌӗ૱ѝⲴ CP4 EPSPS 㳻ⲭⲴᆹޘᙗᐢ㓿㻛བྷ䟿Ⲵᆹޘᙗ䇴 ԧ⹄ウᡰ䇱ᇎDŽ ཆˈ֯⭘ᴰᯠ؞䇒઼ᴤᯠⲴ䗷ǃ䞷ⓦ㳻ⲭ઼哖䉧㳻ⲭᒿࡇᮠᦞᓃ↔ ˄AD_2016˅ǃ∂㍐ᮠᦞᓃ˄TOX_2016˅ǃGenBank 㳻ⲭ䍘ᮠᦞᓃ˄PRT_2016˅ǃ GenBank 㺘䗮ᒿࡇḷㆮᮠᦞᓃ˄EST˅ǃ䶎߇։Ṩ㤧䞨ᮠᦞᓃ˄NT_2016˅ԕ৺ 䶎߇։≘ส䞨ᮠᦞᓃ˄NR_2016˅ሩ MON 87427 ᨂޕս⛩䱴䘁४ฏ䘋㹼ޘ䶒Ⲵ ٷ㔃⛩४㘫䈁㘼ᗇⲴ 14 ޕ㘬ᒿࡇ-ᨂח’⭏⢙ؑᆖ࠶᷀DŽवᤜ˖֯⭘Ӿ 5’ᡆ 3 ᇊᒿࡇԕ৺Ӿ T-DNA 㘫䈁Ⲵ 6 䈫⸱ṶѪỰ㍒ᒿࡇˈ൘ AD_2016ǃTOX_2016 ઼ PRT_2016 ᮠᦞᓃѝ࠶࡛䘋㹼 FASTA ઼ޛ≘ส䞨━ࣘデਓᩌ㍒DŽᩌ㍒㔃᷌㺘 ᇊᒿࡇ઼Ӿ T-DNA 㘫䈁Ⲵ 6 䈫⸱Ṷ൘Ἵ⢙ѝᆈ൘Ⲵٷ᰾ˈ㲭❦⨶䇪к䘉 14 ᓧⲴ∂㍐㳻ⲭ઼ڕ⢙ਟ㜭ᙗᶱվˈণ֯ᆈ൘ˈ䘉Ӌᒿࡇоᐢ⸕ਟ㜭ᖡ૽Ӫ㊫ᡆࣘ ᣇ㩕ޫഐᆀǃ㠤㳻ⲭ઼࣏㜭⍫ᙗ㳻ⲭѻ䰤൷ᰐᱮ㪇ᒿࡇլᙗᡆ਼ⓀᙗDŽሶ 㘬ᒿࡇ൘ EST_2016 ઼ NT_2016 ᮠᦞᓃחս⛩䱴䘁Ⲵสഐ㓴ޕMON 87427 ѝᨂ ѝ䘋㹼 BLASTn ࠶᷀ˈ൘ NR_2016 ᮠᦞᓃѝ䘋㹼 BLASTx ࠶᷀ˈ൷ᵚਁ⧠ MON ˈ㘬สഐ㓴 DNA 䜘ᡆ䱴䘁ᆈ൘ਟ㜭㕆⸱ᯠ㳻ⲭӗ⢙Ⲵ ORFחս⛩ޕᨂ 87427 ҏ⋑ᴹⓀᙗⲴ ORF ഐѪ T-DNA Ⲵᨂޕ㘼㻛ൿDŽ 3.3.1 ∈ᙝ 䇴ԧᯠᕅޕ㳻ⲭ▌൘Ⲵ∂ᙗ઼㠤ᙗԕ৺㟣伏ᆹޘᙗᰦˈ䈕㳻ⲭᱟᴹᆹޘ ˅⭘শਢᱟ䴰㾱㘳㲁Ⲵањ䟽㾱ഐ㍐DŽ↔ཆˈṩᦞഭ䱵伏૱⌅ިငઈՊ˄Codex֯ ᆹޘ䇴ԧᤷই˄Codex Alimentarius, 2003; Codex Alimentarius, 2009˅ˈ㳻ⲭ䍘Ⲵ▌ ൘∂ᙗ䇴ԧ䴰㾱∄䖳ᯠᕅޕ㳻ⲭоᐢ⸕∂㍐઼ᣇ㩕ޫഐᆀⲴ⭏ॆ⢩ᙗᱟާᴹ լᙗˈ䇴ԧⲴ⭏⢙ॆᆖ⢩ᙗवᤜ˖1˅䈕㳻ⲭоᐢ⸕∂㳻ⲭ઼ᣇ㩕ޫഐᆀᡆަ ᓧާᴹн㢟ᖡ૽Ⲵ⭏⢙⍫ᙗ㳻ⲭᱟާᴹᒿࡇլᙗ˗2˅䈕ڕ⢙ᆳሩӪ㊫৺ࣘ 㳻ⲭ൘ପңࣘ⢙⎸ॆ⏢ѝⲴ⎸ॆᙗ˗3˅䈕㳻ⲭᱟާᴹ✝っᇊᙗ˗4˅䈕㳻ⲭሩ ପңࣘ⢙ᱟާᴹᙕᙗ㓿ਓ∂ᙗ˗5˅䈕㳻ⲭ൘⢙ਟ伏⭘䜘࠶ѝᡰঐ∄ֻԕ৺ ሩӪ㊫Ⲵ㟣伏᳤䵢≤ᒣDŽ ṩᦞԕкḷ߶ሩ CP4 EPSPS 㳻ⲭԕ৺ MON 87427 ⦹㊣䘋㹼Ҷ▌൘∂ᙗ䇴ԧˈ 㔃᷌㺘᰾ሩӪ㊫઼ପңࣘ⢙нާᴹ∂ᙗ仾䲙DŽ 81 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᙗޘCP4 EPSPS 㳻ⲭփ⭏⢙Ⲵᆹ˅1 CP4 EPSPS 㳻ⲭⲴփ⭏⢙Ѫ൏༔ṩⱼߌᵶ㧼 CP4 㧼ṚˈഐѪᆳ㜭ӗ⭏ཙ Ⲵǃо㥹⭈㟖Ӣ઼ᙗվⲴ EPSPS 㳻ⲭ㘼֯㺘䗮 CP4 EPSPS 㳻ⲭⲴἽ⢙ਟԕᣇ❦ 㥹⭈㟖˄Padgette et al., 1996˅DŽ㖾ഭᗞ⭏⢙ษޫᓃ˄American Type Culture Collection˅ᐢ㓿ሩ䘉 CP4 㧼Ṛ䘋㹼Ҷ࠶઼䢤ᇊˈᴰ㓸⺞ᇊҾ൏༔ߌᵶ㧼 Ⲵ⭏⨶ሿDŽⴞࡽ䘈⋑ᴹᣕ䚃㺘᰾ԫօаߌᵶ㧼ሩӪ㊫ᡆ㘵ࣘ⢙ާᴹ㠤⯵㜭 ࣋ˈ㘼фߌᵶ㧼а㡜нᱟ㠤Ⓚ˄FAO/WHO, 1991˅DŽ↔ཆˈṩᦞ FAO/WHO˄2001˅ ᣕˈⴞࡽ䘈⋑ਁ⧠ԫօⲴӪ㗔ሩ㓶㧼ᙗ㳻ⲭ䗷DŽ 2˅CP4 EPSPS 㳻ⲭⲴ⭏ॆ⢩ᙗ৺㳻ⲭㅹ਼ᙗ࠶᷀ CP4 EPSPSᱟ⭡455њ≘ส䞨㓴ᡀⲴঅ䬮ཊ㛭ˈ⨶䇪࠶ᆀ䟿Ѫ47.6 kDaDŽӾ ᒿ ࡇкⴻˈCP4 EPSPSоἽ⢙ᶕⓀⲴEPSPSާᴹ25-30%Ⲵլᙗˈо㓶㧼ᶕⓀⲴ EPSPSާᴹ50-90%ⲴլᙗDŽⴞࡽᐢ⸕ⲴᡰᴹᶕⓀEPSPS䞦˄वᤜCP4 EPSPS˅ ൘й㓗ᤃᢁ㔃ᶴк儈ᓖа㠤˄Hammond, et al. 2013˅DŽCP4 EPSPS䞦Ⲵ⤜⢩ᶴ䙐 ᤱᖸ儈ⲴӢ઼ᙗDŽ؍ᗇަሩ㥹⭈㟖нᝏˈն਼ᰦሩཙ❦ᓅ⢙PEP֯ EPSPS 䞦˄वᤜ CP4 EPSPS˅ާᴹ儈ᓖⲴᓅ⢙⢩ᔲᙗDŽⴞࡽᐢ⸕ሩ EPSPS ਈॆDŽ׳䞦ާᴹ⭏⢙ᆖѹⲴᓅ⢙ӵѪ S3P ઼ PEPDŽ㥹⭈㟖н㜭ਁ⭏ EPSPS 䞦 㧭㥹䞨оབྷ㛐ᵶ㧼ᶕⓀ EPSPS 䞦Ⲵ㔃ਸ㜭࣋䶎ᑨᕡˈо S3P ∄ˈ㧭㥹䞨䴰㾱 ˈ䶎ᑨ儈⎃ᓖ˄mM 㓗˅ᴹਟ㜭㻛 EPSPS 䞦ۜॆ˄Gruys, et al. 1992˅DŽ⹄ウ㺘᰾ Ἵ⢙փཙ❦ᆈ൘ S3P ㊫լ⢙ྲ⭢ส㧭㥹䞨ǃཾቬ䞨ǃҼ≒㧭㥹䞨䜭нᱟ EPSPS Ⲵᓅ⢙˄Franz, et al. 1997˅DŽ CP4 EPSPS㳻ⲭⲴ䞦⍫ᙗ઼っᇊᙗਇpH٬ǃᓖ઼ᆀᕪᓖㅹഐ㍐ᖡ૽DŽᴰ 䘲䞦⍫pHѪ9-9.5ˈpH٬䗷儈ᡆ䗷վ䜭Պ䱽վ䞦⍫ᙗˈpHሿҾ5.5ᰦCP4 EPSPS䞦 ⍫ࠐѾᆼޘїཡˈн䗷⭡pHᕅ䎧Ⲵ䞦⍫ਈॆᒦ䶎нਟ䘶˄Padgette et al. 1993˅DŽ CP4 EPSPSሩ✝нっᇊˈᓖ䎵䗷75°CᰦˈCP4 EPSPSⲴ࣏㜭⍫ᙗণ䱽㠣Ự⍻䲀 ԕлˈն࣐✝ᒦнᖡ૽㳻ⲭа㓗㔃ᶴⲴᆼᮤᙗDŽ䘉ㅖਸ✝ࣘ࣋ᆖ⌅ࡉˈণᓖॷ ˈ儈Պ֯㳻ⲭ儈㓗㔃ᶴਈᙗˈᕅ䎧䞦⍫ᙗїཡDŽCP4 EPSPSሩ✝нっᇊⲴ⢩ᙗ㺘᰾ ൘ᐕъ࣐ᐕᶑԦлˈᴰ㓸Ⲵ䖜สഐ⦹㊣ӗ૱ѝнཚਟ㜭ᆈ൘ާᴹ࣏㜭⍫ᙗⲴ CP4 EPSPS㳻ⲭ˄Hammond and Jez 2011˅DŽоᡰᴹިරⲴ㳻ⲭ䞦㊫аṧˈ CP4 EPSPSⲴۜॆ⍫ᙗ䘈ਇ৽ᓄⓦ⏢ѝᆀᕪᓖⲴᖡ૽DŽ↓ᑨⲴ䍞ᆈ㕃ߢ⏢˄1 mM KCl˅ᶑԦлˈCP4 EPSPSⲴ⢩ᔲ⍫ᙗᴰ儈㜭ཏ䗮ࡠ5.5 U/mgDŽCP4 EPSPS Ⲵ⢩ᔲ⍫ᙗ䲿⵰ᆀᕪᓖ໎བྷ㘼ॷ儈ˈ㠣50 mM KClᰦˈ䞦⍫ᙗ㜭ཏ໎࣐2-2.6 ؽ˄Padgette et al. 1993˅DŽ ⼧㔬ਸ֯⭘ㅹ⭥⛩⊹䱽ǃ⺛䞨䬥࠶㓗࠶ǃ⮿≤ᙗቲ᷀ǃ䱤ᆀӔᦒቲ઼᷀ 䞨㓔㔤㍐Ӣ઼ቲ᷀ㅹᯩ⌅ˈӾMON 87427 ᆀⲴ⹄⼘ᨀਆ⢙ѝ㓟ॆᗇࡠ CP4 EPSPS㳻ⲭ˄䈖㿱䲿⭣䈧ҖᨀӔⲴᢰᵟᣕ5˅DŽ䟷⭘ཊ࠶᷀ᢰᵟሩ㓟ॆⲴ Ἵ⢙ⓀCP4 EPSPS㳻ⲭ䘋㹼⭏ॆ⢩ᙗ䢤ᇊˈᡰ⭘࠶᷀ᢰᵟवᤜ˖1˅⭘Bio-Rad㳻 ⲭ࠶᷀㌫㔏⍻ᇊᙫ㳻ⲭ⎃ᓖ˗2˅ॱҼ✧ส⼪䞨䫐-㚊щ✟䞠㜪ࠍ㜦⭥⌣ 82 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 SDS-PAGE˅৺ᇶᓖ䇑䟿˗3˅N-ᵛㄟᒿࡇ࠶᷀˗4˅ส䍘䖵ࣙ◰ݹ䀓᷀⭥伎˄ 㹼ᰦ䰤˄MALDI-TOF˅䍘䉡˗5˅ݽ⯛ঠ䘩઼ᇶᓖ䇑⍻ᇊ࠶᷀˗6˅CP4 EPSPS 䞦⍫ᙗỰ⍻˗7˅㌆สॆ࠶᷀DŽ↔ཆˈ䘈䙊䗷SDS-PAGEՠ㇇н਼ᓖл䍞ᆈа ⇥ᰦ䰤ਾޘ䮯CP4 EPSPS 㳻ⲭⲴ㓟ᓖ৺࠶ᆀ䟿ˈӾ㘼䇴ՠ㓟ॆⲴἽ⢙Ⓚ CP4 EPSPS㳻ⲭⲴっᇊᙗDŽᒦ਼ᰦоབྷ㛐ᵶ㧼㺘䗮㌫㔏ѝ㓟ॆᗇࡠⲴCP4 EPSPS 㳻ⲭ䘋㹼∄䖳DŽ 䟷⭘ᣇ-CP4 EPSPS ᣇփݽ⯛ঠ䘩࠶᷀ǃN-ᵛㄟ⍻ᒿ઼ MALDI-TOF 䍘䉡࠶ ᷀ᢰᵟሩ㜠㳻ⲭ䞦⎸ॆⲴ CP4 EPSPS 㳻ⲭ䘋㹼࠶᷀ˈԕ傼䇱 MON 87427 ѝ CP4 EPSPS 㳻ⲭⲴ䓛ԭDŽ⭘ Bio-Rad 㳻ⲭ࠶᷀㌫㔏⍻ᗇ MON 87427 ᆀѝ㓟ॆ ᗇࡠⲴ CP4 EPSPS Ⲵᙫ㳻ⲭ⎃ᓖѪ 0.1 mg/ml˗䙊䗷㘳傜ᯟӞ㬍 G ḃ㢢 SDS 㚊 щ✟䞠㜪ࠍ㜦Ⲵᇶᓖ࠶᷀⍻ᇊᗇࡠ MON 87427 ޘ䮯 CP4 EPSPS 㳻ⲭⲴ㓟ᓖ઼࠶ ᆀ䟿࠶࡛Ѫ 96%઼ 44.1 kDaDŽ˗N-ᵛㄟᒿࡇ࠶᷀ˈ⺞䇔Ҷ䎧Ⲵ 15 њ≘ส䞨ˈ䞦 䀓㛭⇥㾶ⴆമ䉡㾶ⴆҶ亴ᵏ㳻ⲭᒿࡇⲴ 70.3%˗㓿㜠㳻ⲭ䞦⎸ॆⲴ CP4 EPSPS 㳻ⲭ䘋㹼 MALDI-TOF 䍘䉡࠶᷀ˈӗ⭏Ⲵཊ㛭ᒣ൷䍘䟿Ѫ 47552 Daˈо MON 87427 ѝ cp4 epsps Ṩ㤧䞨ᒿࡇ᧘ሬࠪⲴ CP4 EPSPS 㳻ⲭⲴ⨶䇪㜠㳻ⲭ䞦⎸ ॆ⡷⇥ᱟа㠤ⲴDŽ 䙊䗷ݽ⯛⍫ᙗǃ࠶ᆀ䟿⍻ᇊǃ䞦⍫⍻䈅ǃ㌆สॆ⣦ᘱㅹ࠶᷀ˈ䘋а↕⺞ᇊ CP4 EPSPS 㳻ⲭⲴ⭏ॆ⢩ᙗˈᒦ䇱ᇎབྷ㛐ᵶ㧼ᶕⓀ㳻ⲭо MON 87427 ѝ㓟ॆᗇ ࡠ CP4 EPSPS 㳻ⲭⲴㅹ਼ᙗDŽሩ MON 87427 ᶕⓀ઼བྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭⲴؑਧᕪᓖ䘋㹼ݹᇶᓖ࠶᷀ˈ⽪є㘵ᒣ൷٬ⲴᐞᔲѪ 9.6%ˈ൘䇮ᇊⲴ᧕ ਇḷ߶±35%ԕ˗㘳傜ᯟӞ㬍ḃ㢢Ⲵ SDS-PAGE ⭥⌣࠶᷀㺘᰾ˈMON 87427 ᶕⓀ CP4 EPSPS 㳻ⲭ㺘㿲࠶ᆀ䟿Ѫ 44.1 kDaˈоབྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS ৲ ➗ḷ߶㳻ⲭާᴹ਼Ⲵ䗱〫ս㖞˗䙊䗷⼧䞨䟺᭮ᇎ傼ᶕ⍻ᇊ CP4 EPSPS 㳻ⲭⲴ MON 87427 ᶕⓀ઼བྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ⢩ᔲ⍫ᙗ࠶ˈᔲ䞦⍫ᙗ٬⢩ ࡛Ѫ 8.67 U/mg ઼ 5.41 U/mg˗㓿䢤ᇊˈMON 87427 ᶕⓀⲴ CP4 EPSPS 㳻ⲭ⋑ᴹ 㻛㌆สॆDŽ㔬ਸк䘠㔃᷌ˈMON 87427 ᶕⓀ઼བྷ㛐ᵶ㧼ӗ⭏ CP4 EPSPS 㳻ⲭᱟ ᆈㅜ 14 ཙᰦ˅㳻؍ᇎ䍘ㅹ਼ⲴDŽ↔ཆˈ䙊䗷∄䖳ᇎ傼ᔰ઼㔃ᶏᰦ˄-80ćߠ㇡ ⲭⲴ㓟ᓖ઼࠶ᆀ䟿⍻ᇊ㔃᷌ˈ䇔Ѫ MON 87427 ᨀਆⲴ CP4 EPSPS 㳻ⲭ൘ᇎ傼䗷 ᤱっᇊDŽ؍〻ѝ 3˅CP4 EPSPS 㳻ⲭⲴ䮯ᵏᆹޘᓄ⭘শਢ EPSPS 㳻ⲭ˄5-✟䞷щ䞞䞠-3-⼧䞨䖜〫䞦˅ᒯ⌋ᆈ൘ҾἽ⢙઼ᗞ⭏⢙ѝˈ䇨 ཊ⹄ウ䜭ӾἽ⢙઼ᗞ⭏⢙ѝ࠶ࠪ EPSPS˄Harrison et al., 1996; Haslam, 1993; Klee et al., 1987; Schönbrunn et al., 2001; Steinrücken and Amrhein, 1984˅ˈ৲о㧭 㥹䞨ԓ䉒䙄ᖴDŽቭ㇑ପңࣘ⢙ǃ劬㊫ǃ呏㊫ǃ⡜㹼㊫ࣘ⢙ԕ৺ᰶ㲛փнᆈ൘㧭 㥹䞨ԓ䉒䙄ᖴ˄Alibhai and Stallings; 2001˅ˈҏ⋑ᴹ EPSPS 㳻ⲭˈնԆԜⲴ伏⢙ ѝ EPSPS 䶎ᑨᲞ䙽DŽ㊫ᶕⓀⲴ EPSPS 㳻ⲭⲴ㕆⸱สഐᐢ㓿㻛ݻ䲶ࠪᶕˈ䘉Ӌ ᆸ˄Padgette et al., 1996˅DŽ㓶㧼Ⲵ؍㳻ⲭⲴۜॆ㔃ᶴฏ˄catalytic domain˅儈ᓖ EPSPS 㳻ⲭй㔤㔃ᶴ˄Stallings et al., 1991˅ԕ৺䞦઼ࣘ࣋ॆᆖ৽ᓄᵪࡦ˄Anderson 83 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 and Johnson, 1990˅䜭ᐢ㓿ᖸᾊDŽMON 87427 㺘䗮Ⲵ CP4 EPSPS 㳻ⲭᶕⓀҾ ൏༔ߌᵶ㧼 CP4 㧼Ṛˈᱟ䇨ཊ㠚❦⭼ᒯ⌋ᆈ൘Ⲵ EPSPS 㳻ⲭѝⲴаDŽ䲔Ҷሩ 㥹⭈㟖Ⲵ㙀ਇᙗн਼ཆˈоἽ⢙ѝⲴ EPSPS 㳻ⲭ൘࣏㜭кᇎ䍘ㅹ਼˄Padgette et al., 1996˅DŽ CP4 EPSPS 㳻ⲭާᴹ൘ޘ⨳伏⢙䬮ѝ䮯ᵏᆹޘ⎸䍩ⲴশਢDŽ㠚Ӿ 1996 ᒤᣇ 㥹⭈㟖བྷ䉶୶ъॆἽԕᶕˈਜ਼ᴹ CP4 EPSPS 㳻ⲭⲴߌӗ૱ቡаⴤ൘ޘ⨳伏⢙ 伏⭘DŽਜ਼ᴹ CP4 EPSPS Ⲵᣇ㥹⭈㟖⢙⇿ᒤޘᓄ䬮ѝᆈ൘ᒦ㻛Ӫ㊫઼ࣘ⢙ᆹ 䜭ᴹ 30 ཊњӗ૱൘ 13 њഭᇦἽ䶒〟䎵䗷 7400 зޜ产˄Dill et al., 2008˅DŽц ⭼㤳തᐢ㓿ᴹ䇨ཊ㺘䗮 CP4 EPSPS 㳻ⲭⲴᣇ㥹⭈㟖⢙˄བྷ䉶ǃ⦹㊣ǃ⋩㨌ǃ ỹ㣡ǃ⭌㨌઼㤌㬯ㅹ˅㧧߶୶ъॆἽˈҏ㻛वᤜѝഭ൘Ⲵ䇨ཊഭᇦ䇨ਟ䘋ਓ ⭘࣐ᐕᯉDŽMON 87427 㺘䗮Ⲵ CP4 EPSPS 㳻ⲭо䘉Ӌᐢ㓿㧧ᗇᢩ߶ᒦ୶ъ ᣔ㖢؍ᓄ⭘䘁 20 ᒤⲴᣇ㥹⭈㟖⢙ѝⲴ CP4 EPSPS ᱟ਼ṧⲴDŽ㖾ഭ⧟ຳॆ ˄US-EPA˅ҏᇑḕҶ CP4 EPSPS 㳻ⲭⲴᆹޘᙗˈṩᦞަ൘⁑ᤏ㛳⏢⎸ॆǃоᐢ ⸕∂㍐઼ᣇ㩕ޫഐᆀǃ㠤㳻ⲭⲴ⭏⢙ؑᆖ࠶᷀ǃᙕᙗ㓿ਓ∂ᙗᇎ傼ㅹ㔃઼᷌ U.S. EPA, 1996˅DŽ˄Ⲵˈн䴰㾱൘ߌӗ૱ѝᔪ・ަਜ਼䟿Ⲵᇩ䇨䰸٬ޘᮠᦞˈ䇔Ѫᱟᆹ 㠚 1996 ᒤᣇ㥹⭈㟖⢙୶ъॆἽԕᶕˈᐢᴹ䎵䗷 1000 ӯཤࣘ⢙㻛侢லਜ਼ ᓧ઼⭏ӗ⦷Ⲵڕ⢙ᴹ䖜สഐ⢙˄བྷ䜘࠶ᱟᣇ㥹⭈㟖⢙˅Ⲵ侢ᯉˈ⋑ᴹᖡ૽ࣘ ᣕDŽаㇷ㓿䗷਼㹼䇴䇞ⲴޣҾ侢லਜ਼ᴹ䖜สഐ⢙˄वᤜᣇ㥹⭈㟖⢙˅侢ᯉ ᓧᖡ૽Ⲵ᮷⥞㔬䘠˄Eenennaam & Young, 2014˅ҏᤷࠪˈⴞࡽڕሩࣘ⢙⭏䮯઼ ᓧӗ⭏н࡙ᖡ૽DŽ൘ڕ䘈⋑ᴹԫօֻᆀ㺘᰾ਜ਼ᴹ䖜สഐ⢙侢ᯉՊሩࣘ⢙⭏䮯઼ 䗷৫ 20 ᒤˈޘ⨳ᐢ㓿ᴹ 488 亩⅑ޣҾ CP4 EPSPS 㳻ⲭᆹޘᙗⲴᇑḕᡆ䇴ᇑˈᗇ ᓧ⋑ᴹн㢟ᖡ૽DŽڕ⢙ࠪⲴ㔃䇪൷䇔Ѫ CP4 EPSPS 㳻ⲭሩӪ㊫઼ࣘ 4˅CP4 EPSPS оަᆳᐢ⸕∂㳻ⲭⲴ㔃ᶴլᙗ 㻛䇴ՠ㳻ⲭоᐢ⸕∂㍐ᡆа؍⺞㳻ⲭ▌൘∂ᙗⲴ⭏⢙ؑᆖ࠶᷀ⲴⴞⲴᱟ Ӌᣇ㩕ޫ㳻ⲭ൘ᒿࡇ઼㔃ᶴкнާᴹլᙗDŽ࡙⭘ FASTA ᒿࡇ࠶᷀䖟Ԧˈਟԕ ࠶᷀ CP4 EPSPS 㳻ⲭо∂㍐㳻ⲭᮠᦞᓃѝ㳻ⲭ㔃ᶴⲴլᙗ˄䈖㿱䲿⭣䈧 ҖᨀӔⲴᢰᵟᣕ 6˅DŽFASTA ㇇⌅䲔Ҷ㜭ⴤ᧕ሩ∄㳻ⲭ䍘Ⲵ≘ส䞨ᒿࡇ˄ণ ࡍ㓗㳻ⲭ㔃ᶴ˅ཆˈ䘈㜭⭘Ҿ᧘ሬᴤ儈а㓗Ⲵ㔃ᶴլᙗ˄ণҼ㓗઼й㓗㳻ⲭ㔃 ᶴ˅DŽ൘ᮤњⲴᒿࡇ䟼ᴹ儈ᓖլᙗⲴ㳻ⲭˈᑨᑨᱟ਼Ⓚ㳻ⲭˈ㘼਼Ⓚ㳻ⲭ㓿ᑨ ٬䴰㾱ሿҾᡆㅹҾ 1e-5 ᴹ਼ṧⲴҼ㓗઼й㓗㔃ᶴDŽа㡜㘼䀰ˈєњᒿࡇ䰤Ⲵ Eާ 㻛䇔Ѫᴹᱮ㪇਼ⓀᙗDŽѪҶ䘋㹼ᴤޘ䶒ൠ䇴ԧˈӾ 2016 ᒤᔰˈ䟷⭘ањᶱ E=0.01 䘋㹼⭏⢙ؑᆖ࠶᷀DŽ ᆸⲴ䰸٬؍ㄟ 䪸ሩ MON 87427 Ⲵ⭏⢙ؑᆖ࠶᷀䟷⭘Ⲵ∂㍐㳻ⲭᮠᦞᓃѪ TOX_2016DŽ TOX_2016 ᮠᦞᓃᱟӾ PRT_2016 ᮠᦞᓃѝ䗷└䲔৫ਟ㜭Ⲵ䶎∂㍐㳻ⲭਾᖒᡀⲴ ӊᮠᦞᓃDŽTOX_2016 वᤜҶ 18,554 њ㳻ⲭᒿࡇDŽ⭘ CP4 EPSPS 㳻ⲭᒿࡇ൘ ٬Ѫ 0.51DŽ-TOX_2016 ᮠᦞᓃѝ䘋㹼ᩌ㍒ˈ䇴࠶ᴰ儈Ⲵ∄ሩѪ GI-406373209ˈަ E ٬Ѫ 0.01 ᰦˈ⋑ᴹᗇࡠ ᆸ E؍٬䗷儈ˈ∄ሩ㔃᷌нާᴹ᭸ѹDŽ䟷⭘-⭡Ҿަ E 84 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ԫօ∄ሩ㔃᷌˄ᢰᵟᣕ 6˅DŽഐ↔ਟԕᗇࠪ㔃䇪䇔Ѫ MON 87427 о TOX_2016 ᮠᦞᓃѝⲴ∂㍐㳻ⲭ䜭нާᴹᒿࡇ઼㔃ᶴкⲴլᙗDŽ ਖཆˈ䘈⭘ CP4 EPSPS 㳻ⲭᒿࡇ൘ PRT_2016 ᮠᦞᓃ˄ᶕⓀҾ NCBI 㖁ㄉл 䖭Ⲵ GenBank 㳻ⲭᮠᦞᓃ 211 ᴤᯠ⡸ᵜˈवਜ਼ᴹ 49,462,283 њާᴹ⭏⢙⍫ᙗⲴ 㳻ⲭᒿࡇ˅ѝ䘋㹼ᩌ㍒ˈ䇴࠶ᴰ儈Ⲵ∄ሩѪ൘ 455 њ≘ส䞨㤳തާᴹ 100.00% ٬Ѫ 6.9e-173DŽ䘉а㔃᷌ӵ-լᙗⲴᒿࡇѪ CP4 EPSPS˄GI-347666444˅ˈަ E ᓧⲴڕ䇱ᇎҶ CP4 EPSPS 㳻ⲭⲴ䓛ԭˈн㜭㺘᰾ CP4 EPSPS 㳻ⲭᵜ䓛ާᴹডᇣ 0.01 䘋㹼ㆋ䘹ᰦˈᗇࡠҶᴤཊⲴ∄ሩ㔃᷌ˈަѝབྷ䜘=٬ ᆸ E؍⭏⢙⍫ᙗDŽ䟷⭘ ࠶઼ EPSPS 㳻ⲭа㠤DŽ∄ሩ㔃᷌䇶࡛ࠪᶕ㠚ߌᵶ㧼 CP4 㧼ṚⲴ CP4 EPSPS о઼ ᶕ㠚ަԆ⢙ EPSPS 㳻ⲭⲴլᙗн㜭㺘᰾ CP4 EPSPS 㳻ⲭᆈ൘▌൘н㢟⭏⢙ ▌⍫ᙗDŽഐ↔ˈ֯⭘ PRT_2016 ᮠᦞᓃ⋑ᴹỰ㍒㔃᷌㺘᰾ CP4 EPSPS 㳻ⲭᆈ൘ ൘Ⲵᣇ㩕ޫഐᆀ⍫ᙗDŽ 5˅CP4 EPSPS 㳻ⲭփཆ⁑ᤏ⎸ॆᇎ傼 Harrison ㅹ˄ 1996˅䇱᰾Ҷ CP4 EPSPS 㳻ⲭ㜭൘⁑ᤏ⎸ॆᶑԦлՊ㻛䗵䙏⎸ ॆᦹDŽṩᦞ Western ঠ䘩࠶᷀ˈCP4 EPSPS ൘⁑ᤏ㛳⏢⎸ॆ㌫㔏ѝ 15 s ਾቡᰐ⌅ Ự⍻ࡠDŽ ᆏኡ䜭ޜਨሩ CP4 EPSPS 㳻ⲭ൘փ⁑ᤏ㛳⏢˄SGF˅⎸ॆᇎ傼ҏ䇱ᇎҶ к䘠㿲⛩DŽ䈕䈅傼䟷⭘ഭ䱵⭏ભ、ᆖ⹄ウᡰ˄ILSI˅ޜᐳⲴḷ߶ᯩ⌅˄Thomas et al., 2005˅DŽ਼ Harrison ㅹ˄1996˅ԕࡽⲴᇎ傼аṧˈᵜ亩⹄ウҏ䟷⭘Ҷབྷ㛐ᵶ㧼 ⭏ӗⲴ CP4 EPSPS 㳻ⲭˈ⎸ॆ⦷䟷⭘ SDS-PAGE ⭥⌣ࠍ㜦ḃ㢢ǃ㳻ⲭ䍘ঠ䘩઼ CP4 EPSPS 䞦৽ᓄ⍫ᙗ䢤ᇊ䘋㹼䇴ՠDŽ ᇎ傼㔃᷌䇱ᇎҶ CP4 EPSPS 㳻ⲭ൘փཆ⁑ᤏⲴ SGF 䟼䗵䙏㻛⎸ॆDŽ SDS-PAGE ࠍ㜦ḃ㢢㔃᷌㺘᰾ˈ98%ԕкⲴ CP4 EPSPS 㳻ⲭ൘ 15 s 㻛䗵䙏⎸ ॆ˄മ 21˅DŽWestern ᵲӔ࠶᷀㔃᷌㺘᰾ˈ95%ԕкⲴ CP4 EPSPS 㳻ⲭ൘ 15 s ণᰐ⌅Ự⍻˄മ 22˅DŽ਼ṧˈCP4 EPSPS 㳻ⲭ൘ SGF 䟼ษޫнࡠ 15 sˈަ⍫ᙗ䱽 վࡠ 10%ԕл˄㺘 18˅DŽᙫѻˈփཆ⁑ᤏ⎸ॆᇎ傼Ⲵ㔃᷌ਟԕᯝᇊ CP4 EPSPS ᓧӗ⭏ড䲙DŽڕ㳻ⲭ㜭൘⁑ᤏ㛳⏢䟼䗵䙏㻛䱽䀓ˈᡰԕнਟ㜭ሩӪփ 6˅CP4 EPSPS 㳻ⲭⲴ✝っᇊᙗᇎ傼 ࣐✝༴⨶൘⦹㊣㊭㋂ԕ৺ਜ਼ᴹ⦹㊣㊭㋂Ⲵӗ૱࣐ᐕ䗷〻ѝᓄ⭘䶎ᑨᒯ⌋ˈഐ ↔䇴ՠ CP4 EPSPS 㳻ⲭሩ✝༴⨶Ⲵっᇊᙗҏᱟ䶎ᑨ䟽㾱ⲴDŽ ✝༴⨶ᇎ傼⭘Ⲵ CP4 EPSPS ᱟ⭡བྷ㛐ᵶ㧼⭏ӗⲴˈ✝༴⨶ᓖѪ 25°Cǃ37°Cǃ 55°Cǃ75°C ઼ 95°Cˈ༴⨶ᰦ䰤Ѫ 15 min ᡆ㘵 30 minDŽ䇴ԧⲴᇩवᤜ˖1˅࣐ ✝༴⨶ሩ CP4 EPSPS 㳻ⲭ䞦⍫࣋Ⲵᖡ૽˗2˅࣐✝༴⨶ሩ㳻ⲭᆼᮤᙗⲴᖡ૽DŽ ൘ 25°Cǃ37°Cǃ55°Cǃ75°C ઼ 95°C ᓖᶑԦл࣐✝༴⨶ 15 ઼ 30 min ਾˈ CP4 EPSPS 㳻ⲭⲴ⍫ᙗ⍻䈅㔃᷌࠶࡛㿱㺘 19 ઼㺘 20DŽᖃԕ 25ºCǃ37ºC ઼ 55ºC ࣐✝ 15 min ਾ㿲ሏࡠ CP4 EPSPS ⍫ᙗ〽ᴹ䱽վˈ࠶࡛䱽㠣ሩ➗Ⲵ 81%ǃ84%઼ 70%˗ն࣐✝ 30 min ਾˈ25ºC ༴⨶Ⲵ CP4 EPSPS ⍫ᙗ⋑ᴹਈॆˈ37ºC ༴⨶㓴Ⲵ 85 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䞦⍫䱽㠣ሩ➗ⲴⲴ 88%ˈ㘼 55ºC ༴⨶㓴Ⲵ CP4 EPSPS 㺘⧠Ѫབྷᑵл䱽ˈӵѪሩ ➗Ⲵ 25%DŽн䇪ᱟ༴⨶ 15 min 䘈ᱟ༴⨶ 30 minˈ75°C ઼ 95°C ᓖᶑԦлⲴ CP4 EPSPS 䞦⍫൷վҾỰ⍻䲀DŽ SDS-PAGE ࠶᷀㔃᷌㺘᰾ˈ৲➗ḷ߶઼ሩ➗⢙वਜ਼аᶑ㺘㿲࠶ᆀ䟿㓖Ѫ 43.8 kDa ⲴѫᶑᑖˈሩᓄҾ CP4 EPSPS 㳻ⲭ˄മ 23 ઼മ 24˅DŽ൘ᡰᴹ༴⨶ᰦ䰤 ⌣ˈ༴⨶ᓖᶑԦлˈṧ૱Ⲵ 43.8 kDa ᶑᑖؑਧᕪᓖ⋑ᴹ᰾ᱮⲴ䱽վ˄മ 23઼ 䚃 3~7˗മ 24ˈ⌣䚃 2~7˅DŽ к䘠㔃᷌㺘᰾ˈ࣐✝༴⨶ӵՊ֯ CP4 EPSPS 㳻ⲭⲴ䞦⍫ᙗ䱽վˈնнՊ֯ CP4 EPSPS 㳻ⲭ䱽䀓DŽഐ↔ˈнՊᖒᡀᯠⲴ㳻ⲭ⡷⇥㘼ᑖᶕᯠⲴ▌൘㠤仾䲙DŽ 86 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 བྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ 㛳㳻ⲭ䞦 മ21. SDS-PAGEࠍ㜦⭥⌣࠶᷀˖CP4 EPSPS㳻ⲭ൘⁑ᤏ㛳⏢˄SGF˅ѝⲴ⎸ॆ 䟷⭘ 10-20%㚊щ✟䞠㜪ࠍ㜦⭥⌣˄SDS-PAGE˅࠶㳻ⲭˈ㕃ߢ⏢Ѫ tricine 㕃ߢ⏢ˈ㜦փӞ㬍 Gḃ㢢DŽสҾ⎸ॆࡽⲴ⎃ᓖˈ⇿⌣䚃кṧ CP4 EPSPS 㳻ⲭ 500 ngDŽ ⌣䚃 ṧ૱᧿䘠 ⎸ॆᰦ䰤 1 ࠶ᆀ䟿ḷ䇠 2 ᰐ㛳㳻ⲭ䞦Ⲵᇎ傼ሩ➗˄P0˅ 0s 3 ᰐ CP4 EPSPS Ⲵᇎ傼ሩ➗˄N0˅ 0s 4 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=0 0s 5 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=1 15s 6 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=2 30s 7 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=3 1min 8 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=4 2min 9 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=5 4min 10 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=6 8min 11 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=7 15min 12 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=8 30min 13 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=9 60min 14 ᰐ CP4 EPSPS Ⲵᇎ傼ሩ➗˄N9˅ 60 min 15 ᰐ㛳㳻ⲭ䞦Ⲵᇎ傼ሩ➗˄P9˅ 60 min 87 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 བྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ മ22. Western㳻ⲭ䍘ঠ䘩࠶᷀˖CP4 EPSPS㳻ⲭ൘⁑ᤏ㛳⏢˄SGF˅ѝⲴ⎸ॆ 䟷⭘ 10-20%㚊щ✟䞠㜪ởᓖࠍ㜦˄SDS-PAGE˅࠶㳻ⲭDŽสҾ⎸ॆࡽⲴ⎃ ᓖˈ⇿⌣䚃кṧ CP4 EPSPS 㳻ⲭ 1ngDŽ ⌣䚃 ṧ૱᧿䘠 ⎸ॆᰦ䰤 1 ࠶ᆀ䟿ḷ䇠 2 ᰐ㛳㳻ⲭ䞦Ⲵᇎ傼ሩ➗˄P0˅ 0s 3 ᰐ CP4 EPSPS Ⲵᇎ傼ሩ➗˄N0˅ 0s 4 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=0 0s 5 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=1 15s 6 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=2 30s 7 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=3 1min 8 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=4 2min 9 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=5 4min 10 ൘ SGF 䟼Ⲵ CPu4 EPSPS 㳻ⲭˈT=6 8min 11 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=7 15min 12 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=8 30min 13 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=9 60min 14 ᰐ CP4 EPSPS Ⲵᇎ傼ሩ➗˄N9˅ 60 min 15 ᰐ㛳㳻ⲭ䞦Ⲵᇎ傼ሩ➗˄P9˅ 60 min 88 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ 23. ࣐✝༴⨶ 15 ࠶䫏ਾ CP4 EPSPS Ⲵ SDS-PAGE Ự⍻ ࣐✝༴⨶ਾⲴ CP4 EPSPS ൘ Tris-⭈≘䞨 4-20%㚊щ✟䞠㜪ࠍ㜦⭥⌣к࠶ ˄3.2 μg ᙫ㳻ⲭ˅ˈ㜦փӞ㬍 G ḃ㢢DŽ࠶ᆀ䟿অսѪ˄kDa˅DŽ ⌣䚃 ṧ૱઼༴⨶᧿䘠 㳻ⲭ䟿˄μg˅ 1 ᇭ㤳ത࠶ᆀ䟿ḷ䇠 4.5 2 CP4 EPSPS Ѫ࣐✝༴⨶˄0ºC˅ 3.2 3 CP4 EPSPS 25 ºC ✝༴⨶ 3.2 4 CP4 EPSPS 37 ºC ✝༴⨶ 3.2 5 CP4 EPSPS 55 ºC ✝༴⨶ 3.2 6 CP4 EPSPS 75 ºC ✝༴⨶ 3.2 7 CP4 EPSPS 95 ºC ✝༴⨶ 3.2 8 ḷ߶ CP4 EPSPS 㳻ⲭ 3.2 9 ḷ߶ CP4 EPSPS 㳻ⲭ 0.32 10 ᇭ㤳ത࠶ᆀ䟿ḷ䇠 4.5 89 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ24. ࣐✝༴⨶30࠶䫏ਾCP4 EPSPSⲴSDS-PAGEỰ⍻ ࣐✝༴⨶ਾⲴ CP4 EPSPS ൘ Tris-⭈≘䞨 4-20%㚊щ✟䞠㜪ࠍ㜦⭥⌣к࠶ ˄3.2 μg ᙫ㳻ⲭ˅ˈ㜦փӞ㬍 G ḃ㢢DŽ࠶ᆀ䟿অսѪ˄kDa˅DŽ ⌣䚃 ṧ૱઼༴⨶᧿䘠 㳻ⲭ䟿˄μg˅ 1 ᇭ㤳ത࠶ᆀ䟿ḷ䇠 4.5 2 CP4 EPSPS 25 ºC ✝༴⨶ 3.2 3 CP4 EPSPS 37 ºC ✝༴⨶ 3.2 4 CP4 EPSPS 55 ºC ✝༴⨶ 3.2 5 CP4 EPSPS 75 ºC ✝༴⨶ 3.2 6 CP4 EPSPS 95 ºC ✝༴⨶ 3.2 7 CP4 EPSPS Ѫ࣐✝༴⨶˄0ºC˅ 3.2 8 ḷ߶ CP4 EPSPS 㳻ⲭ 3.2 9 ḷ߶ CP4 EPSPS 㳻ⲭ 0.32 10 ᇭ㤳ത࠶ᆀ䟿ḷ䇠 4.5 90 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘18. ൘⁑ᤏ㛳⏢˄SGF˅ѝ⎸ॆਾབྷ㛐ᵶ㧼⭏ӗⲴCP4 EPSPS㳻ⲭⲴ∄⍫ᙗ ∄⍫ᙗ ṧ૱઼༴⨶᧿䘠 (䞦⍫অս/mg 㳻ⲭ) ᰐ㛳㳻ⲭ䞦Ⲵᇎ傼ሩ➗ษޫ 0s 4.92 ᰐ㛳㳻ⲭ䞦Ⲵᇎ傼ሩ➗ษޫ 60.min 2.10 བྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ൘ SGF 䟼ษޫ 0s 5.63 བྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ൘ SGF 䟼ษޫ 15 s 0.27 བྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ൘ SGF 䟼ษޫ 30 s 0.15 བྷ㛐ᵶ㧼ӗ⭏Ⲵᴹ CP4 EPSPS 㳻ⲭ൘ SGF 䟼ษޫ 60 s 0.15 ᰐ CP4 EPSPS Ⲵᇎ傼ሩ➗ษޫ 0s 0.02 ᰐ CP4 EPSPS Ⲵᇎ傼ሩ➗ษޫ 60 min 0.05 㕃ߢ⏢オⲭ 0.01 㺘19. ࣐✝༴⨶15࠶䫏ਾCP4 EPSPS䞦⍫࣋ CP4 EPSPS ࣏㜭⍫ᙗ˄U/mg˅ ༴⨶ᓖ ሩ䞦⍫ 3 ˄ᒣ൷ᮠ 1 ±SD2˅ ᵚ࣐✝˄0ºC˅ 6.03 ± 0.29 100% 25 ºC 4.88 ± 0.24 81% 37 ºC 5.08 ± 0.33 84% 55 ºC 4.22 ± 0.12 70% 75 ºC < LOD4 <3% 95 ºC < LOD4 <3% ˗ᇊѪ࣐✝༴⨶Ⲵ CP4 EPSPS 䞦⍫Ѫ 100%ٷ n=2˗2 SD = ḷ߶ᐞ˗3 1 4 LOD ѪỰ⍻л䲀ˈվҾ LOD Ⲵ CP4 EPSPS 䞦⍫ᇊѹѪк䘠 3 њ✝༴⨶ḷ߶ᐞⲴᒣ൷DŽ 㺘20. ࣐✝༴⨶30࠶䫏ਾCP4 EPSPS䞦⍫࣋ CP4 EPSPS ࣏㜭⍫ᙗ˄U/mg˅ ༴⨶ᓖ ሩ䞦⍫ 3 ˄ᒣ൷ᮠ 1 ±SD2˅ ᵚ࣐✝༴⨶˄0ºC˅ 2.8 ± 0.26 100% 25 ºC 3.1 ± 0.23 110% 37 ºC 2.5 ± 0.05 88% 55 ºC 0.70 ± 0.09 25% 75 ºC < LOD4 <8% 95 ºC < LOD4 <8% ˗ᇊѪ࣐✝༴⨶Ⲵ CP4 EPSPS 䞦⍫Ѫ 100%ٷ n=2˗2 SD = ḷ߶ᐞ˗3 1 4 LOD ѪỰ⍻л䲀ˈվҾ LOD Ⲵ CP4 EPSPS 䞦⍫ᇊѹѪк䘠 3 њ✝༴⨶ḷ߶ᐞⲴᒣ൷DŽ 91 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 7˅CP4 EPSPS 㳻ⲭⲴᙕᙗ㓿ਓ∂ᙗᇎ傼 ᣇ㥹⭈㟖⦹㊣ MON 87427 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭоަᆳᐢ㓿୶ъॆཊᒤ Ⲵᣇ㥹⭈㟖⢙ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭᱟаṧⲴˈࡠⴞࡽѪ→ˈᐢ㓿୶ъॆ ᓄ⭘Ⲵ䖜 cp4 epsps สഐᣇ㥹⭈㟖⢙ᴹ 6 ˈवᤜ㤌㬯ǃ⋩㨌ǃ⦹㊣ǃỹ㣡ǃ བྷ䉶઼⭌㨌DŽ⭡Ҿ㺘䗮Ⲵ㳻ⲭ਼ˈᒦфᐢ䇱ᇎἽ⢙ᶕⓀ CP4 EPSPS 㳻ⲭоབྷ 㛐ᵶ㧼ᶕⓀ㳻ⲭⲴㅹ਼ᙗˈ䘉Ӌ䖜สഐ⢙ᆹޘᙗ䇴ԧᡰ⭘Ⲵ㳻ⲭ㓿ਓᙕᙗ∂ᙗ 䈅傼ᣕ൷䟷⭘ Hammond Ҿ 1993 ᆼᡀⲴ CP4 EPSPS 㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼DŽ 㳻ⲭ∂㍐а㡜ᱟ䙊䗷ᙕᙗ⭘ᵪࡦӗ⭏∂ᙗˈ᧕䀖ਾࠐሿᰦ㠣ࠐཙቡՊ㺘 ⧠ࠪн䘲৽ᓄˈа㡜нՊ䎵䗷аઘ˄Sjoblad et al., 1992; Pariza and Johnson, 2001; Hammond and Fuchs, 1998˅DŽᐢ⸕ⲴἽ⢙㳻ⲭ∂㍐䜭ᱟ൘ᮠሿᰦӗ⭏∂ᙗˈ∄ ྲ˖㬆哫∂㍐ᱟ 3-24 ሿᰦǃ㓒䉶∂㍐ᱟ 6-8 ሿᰦǃἽ⢙ࠍ䳶㍐ A ѪሿҾ 3 ሿᰦDŽ 㘼ᐢ⸕Ⲵ㓶㧼㳻ⲭ∂㍐਼ṧՊ൘ᖸ⸝ᰦ䰤㺘⧠ࠪ∂ᙗ⯷⣦ˈྲ䴽ҡ⯵∂Ѫ 1-5 ཙǃ 㪑㨴⨳㧼㛐∂㍐ B Ѫ 1-6 ሿᰦǃ㚹∂㧼∂㍐Ѫ 18-36 ሿᰦDŽ Hammond˄1993˅࡙⭘བྷ㛐ᵶ㧼㺘䗮㓟ॆ㳻ⲭˈԕ 49ǃ154 ઼ 572 mg/kg bwt ޡ 3 њࡲ䟿ሩ 10 ਚ䳴ᙗ઼ 10 ਚ䳼ᙗ CD-1 ሿ啐䘋㹼 CP4 EPSPS 㳻ⲭ♼㛳༴⨶DŽ オⲭሩ➗㓴⭘ 50 mM ⻣䞨≒䫐㕃ߢ⏢༴⨶䳴ᙗ઼䳼ᙗሿ啐 10 ਚˈࡲ䟿Ѫ 33.33 ml/kg bwtDŽ䱤ᙗሩ➗㓴⭘⢋㹰ⲭ㳻ⲭ˄BSA˅༴⨶ 10 ਚ䳴ᙗ઼ 10 ਚ䳼 ڕ઼⦷ᙗ CD-1 ሿ啐ˈ♼㛳ࡲ䟿Ѫ 363 mg/kg bwtDŽᇎ傼ᵏ䰤ˈ⇿ཙє⅑䈳ḕ↫ӑ ᓧ⣦ߥDŽ༴⨶ㅜ 0 ཙ઼ㅜ 7 ཙ࠶࡛䘋㹼а⅑䈖㓶ⲴѤᒺ㿲ሏᒦ⍻ᇊփ䟽઼䘋伏䟿DŽ ⭡Ҿ⋑ᴹ㿲ሏࡠн㢟৽ᓄˈㅜ 8 ཙ༴↫ሿ啐ᒦ䘋㹼⯵⨶ᆖ㿲ሏˈ㔃᷌ҏᵚਁ⧠ᔲ ᑨ˄㿱ᵜ⭣䈧Җлᢰᵟᣕ 10˅DŽHammond˄1993˅⹄ウѝ 7 ཙ㿲ሏᵏ⏥ⴆ Ҷབྷཊᐢ⸕㳻ⲭ∂㍐ਁᥕ∂ᙗⲴᴹ᭸ᰦ䰤ˈণ֯䘉ᰦ㓸→䈅傼ˈҏᓄ䈕ਟԕ⺞䇔 CP4 EPSPS 㳻ⲭ൘䈅傼ࡲ䟿лнާᴹ∂ᙗDŽഐ↔ˈCP4 EPSPS 㳻ⲭԕᇎ傼ѝⲴ ᴰ儈ࡲ䟿 572 mg/kg bwt ♼㛳ᰦˈ⋑ᴹሩሿ啐ӗ⭏ԫօн࡙ᖡ૽DŽਟԕ䇔Ѫ CP4 EPSPS Ⲵᰐ᰾ᱮᦏᇣ⭘ࡲ䟿˄NOAEL˅㠣ቁѪ 572 mg/kg bwtDŽ ൘ Hammond˄1993˅Ⲵ䈅傼ਾˈᆏኡ䜭ޜਨ䘈䪸ሩᣇ㥹⭈㟖⦹㊣ NK 603 ѝⲴ CP4 EPSPS 㳻ⲭ䘋㹼Ҷਖཆа亩㓿ਓᙕᙗ∂ᙗ䈅傼˄Bonnette, 2002˅DŽ Bonnette˄2002˅䈅傼ѝⲴ CP4 EPSPS 㳻ⲭо Hammond˄1993˅ᡰ⭘㳻ⲭӵ ᐞањ≘ส䞨ˈ਼Ⓚᙗ䗮ࡠ 99.9%˄㿱к㺘˅DŽBonnette˄2002˅൘䈅傼ѝ䟷⭘ Ⲵ㳻ⲭ♼㛳ࡲ䟿Ѫ 817 mg/kgˈѤᒺ㿲ሏᵏѪ 14 ཙˈ਼ṧ⋑ᴹ㿲ሏࡠ CP4 EPSPS ൘䈅傼ᴰ儈ࡲ䟿≤ᒣлሩሿ啐ާᴹн㢟᭸ᓄˈ䈅傼ᡰᗇ㔃䇪о Hammond˄1993˅ 㔃䇪аṧDŽBonnette˄2002˅Ⲵ䈅傼ᣕᐢ䲿ᣇ㥹⭈㟖⦹㊣ NK 603 Ⲵᆹޘ䇴ԧ ⭣䈧䙂Ӕࡠޘ⨳ഭᇦⲴ⌅㿴ⴁ㇑ᵪᶴˈᒦ㻛ᇑḕ઼ᢩ߶DŽ 2015 ᒤˈ⭡Ҿߌъ䜘䇴ᇑуᇦሩ CP4 EPSPS 㳻ⲭሿ啐ᙕᙗ∂ᙗ⹄ウᵚѕṬ 䚥ᗚߌъ䜘ޣҾᙕᙗ∂ᙗ䇴ԧᯩ⌅ᆈ൘亮㲁ˈᆏኡ䜭ޜਨ⅑ሩ CP4 EPSPS 㳻 ⲭ䘋㹼Ҷሿ啐㓿ਓ♼㛳ᙕᙗ∂ᙗ䈅傼DŽSmedley˄2015˅ㅹӪ࡙⭘བྷ㛐ᵶ㧼㺘䗮 㓟ॆⲴ CP4 EPSPS 㳻ⲭԕ㓿ਓᯩᔿԕ 2000 mg/kg փ䟽ࡲ䟿অ⅑♼㛳䳼䳴 10 ਚ CD-1 ሿ啐DŽ਼ᰦ䘋㹼䈅傼Ⲵ䘈ᴹⓦࡲሩ➗㓴ԕ৺ԕ਼ㅹࡲ䟿ሿ⢋㹰㳻ⲭ ˄BSA˅♼㛳Ⲵ㳻ⲭሩ➗㓴DŽᡰᴹࣘ⢙൘♼㛳ࡽ൷⾱伏༴⨶ˈ♼㛳ਾ⇿ཙє⅑㿲 92 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ሏሿ啐ᱟ↫ӑᡆᱟ㺘⧠ࠪѝ∂⯷⣦ˈ㿲ሏᵏѪ 14 ཙDŽㅜ 14 ཙᰦሶᡰᴹࣘ⢙༴ ↫ˈ䘋㹼བྷփ䀓ࢆDŽ⹄ウ㔃᷌㺘᰾ˈᰐ䇪Ӿሿ啐ᆈ⍫⦷ǃѤᒺ㺘⧠ǃփ䟽ǃփ䟽 ໎࣐ǃ伏⢙⎸㙇ᡆབྷփ⯵⨶ᆖᯩ䶒ⴻˈ൷ᵚਁ⧠о༴⨶ޣⲴн㢟৽ᓄDŽഐ↔ˈ 䙊䗷䈕ሿ啐ᙕᙗ∂ᙗ⹄ウᗇࡠⲴ CP4 EPSPS 㳻ⲭⲴᰐ᰾ᱮᦏᇣ⭘ࡲ䟿 ˄NOAEL˅㠣ቁѪ⹄ウᴰ儈ࡲ䟿 2000 mg/kg bwˈ⭡↔ՠ㇇ᗇࡠ䙊䗷㟣伏ᩴޕ MON 87427 ѝ CP4 EPSPS 㳻ⲭⲴ᳤䵢䲀˄MOE˅儈䗮 2.76 × 106DŽSmedley˄2015˅ Ⲵ䈖㓶䈅傼㔃᷌䈧৲㿱ᵜ⭣䈧Җлᢰᵟᣕ 14DŽ 2016 ᒤࡍˈᆏኡ䜭ޜਨ⅑ሩ CP4 EPSPS 㳻ⲭ䘋㹼Ҷሿ啐㓿ਓ♼㛳ᙕᙗ∂ ᙗ䈅傼˄ᢰᵟᣕ 15˅DŽSmedley˄2016˅ㅹӪ࡙⭘བྷ㛐ᵶ㧼㺘䗮㓟ॆⲴ CP4 EPSPS 㳻ⲭԕ㓿ਓᯩᔿԕ 5000 mg/kg փ䟽ࡲ䟿অᰕє⅑♼㛳䳼䳴 10 ਚ CD-1 ሿ啐ˈ 㿲ሏ 14 ཙDŽо Smedley˄2015˅⹄ウаṧˈSmedley˄2016˅䈅傼䇮䇑ѫ㾱ᦞ EPA OPPTS 870.1100ˈҏ┑䏣ߌъ䜘亱ਁⲴޣḷ߶ NY-2031-16-2013DŽ⹄ウѝ ൷ᵚਁ⧠ᇎ傼ࣘ⢙ᴹԫօо CP4 EPSPS 㳻ⲭޣⲴн㢟৽ᓄDŽഐ↔ˈк䘠ཊ⅑ ᓧڕ䘋㹼Ⲵᡰᴹᙕᙗਓᴽ∂ᙗ⹄ウ㔃᷌৽༽䇱ᇎˈCP4 EPSPS 㳻ⲭሩପңࣘ⢙Ⲵ ⋑ᴹԫօн㢟ᖡ૽DŽ 8˅90 ཙབྷ啐லޫ䈅傼 ਇߌъ䜘ငᢈˈ2014 ᒤߌъ䜘䖜สഐ⭏⢙伏⭘ᆹޘⴁⶓỰ傼⍻䈅ѝᗳ˄ཙ ⍕˅᳘ཙ⍕ᐲ⯮⯵亴䱢᧗ࡦѝᗳሩ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ MON 87427 ৺ަ䶎䖜สഐᑨ㿴⦹㊣ሩ➗ EXP262 䘋㹼Ҷᣇ㩕ޫഐᆀਜ਼䟿Ự⍻઼ 90 ཙབྷ啐ޘ 伏૱லޫ䈅傼DŽ ᣇ㩕ޫഐᆀਜ਼䟿Ự⍻㔃᷌㺘᰾ˈ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ MON 87427 ѝἽ䞨ਜ਼䟿Ѫ 20.3 mg/gˈ䶎䖜สഐᑨ㿴⦹㊣ EXP262 ѝἽ䞨ਜ਼䟿Ѫ 20.2 mg/gˈ є㘵⋑ᴹᱮ㪇ᐞᔲDŽ ሶ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ MON 87427 ઼䶎䖜สഐӢᵜ⦹㊣ EXP262 侢ᯉ˄∄ֻѪ 50% ǃ25% ǃ12.5%˅ல侢བྷ啐 90 ཙˈல侢ᵏ䰤ࣘ⢙⍫ޕ᧪࠶࡛ ࣘ㠚ྲˈ㻛∋ᴹݹ⌭ˈ啫ǃǃਓ㞄ᰐᔲᑨ࠶⋼⢙DŽ㔏䇑㔃᷌㺘᰾䜘࠶ᑨ㿴ሩ➗ ৺䖜สഐਇ䈅⢙㓴ࣘ⢙䘨伏䟿оᑨ㿴ส侢ᯉሩ➗㓴∄䖳ˈᴹ㔏䇑ᆖѹ ˄P<0.05˅ˈն൷ᰐࡲ䟿৽ᓄޣ㌫ˈᵚ㿱ަ⭏⢙ᆖѹDŽަ։㓴ࣘ⢙փ䟽ǃ䘋 伏䟿ǃᙫ໎䟽ǃᙫ䘋伏䟿઼ᙫ伏⢙࡙⭘⦷оᑨ㿴ส侢ᯉሩ➗㓴∄䖳ˈᐞᔲ൷ᰐ 㔏䇑ᆖѹ˄P>0.05˅DŽ ᇎ傼ѝᵏ઼ᇎ傼ᵛᵏ亩㹰⏢ᆖᤷḷ˄㓒㓶㜎䇑ᮠǃ㹰㓒㳻ⲭǃ㹰ሿᶯǃⲭ 㓶㜎䇑ᮠ৺࠶㊫˅ǃ㹰⭏ॆᤷḷ˄㹰щ≘䞨≘ส䖜〫䞦ǃཙ䰘ߜ≘䞨≘ส䖜〫 䞦ǃቯ㍐≞ǃ㛶പ䞷ǃ⭈⋩й䞟ǃ㹰㌆ǃң䞨㝡≒䞦ǃᙫ㳻ⲭǃⲭ㳻ⲭǃ㚼䞀ǃ ᙗ⼧䞨䞦˅൷൘ᵜỰ⍻অս↓ᑨ٬㤳തDŽࣘ⢙䀓ࢆᰦˈབྷփ㿲ሏᵚਁ⧠ᔲᑨDŽ⻡ 䳴ᙗࣘ⢙Ӣѝ㓴㛮/փ∄վҾሩ➗㓴ˈӢ儈㓴㛍/փ∄儈Ҿሩ➗㓴ˈ䖜վ㓴㛍/փ∄ 儈Ҿሩ➗㓴ˈ䖜վ㓴ᗳ/փ∄儈ҾӢվ㓴ˈ䖜ѝ㓴㛮к㞪/փ∄儈ҾӢѝ㓴ˈᐞᔲ ᴹ㔏䇑ᆖѹ˄P<0.05˅DŽ䳼ᙗࣘ⢙Ӣ儈㓴㜨㞪/փ∄ǃ㛮/փ∄儈Ҿሩ➗㓴ˈ䖜 ѝ㓴㛍/փ儈Ҿሩ➗㓴ˈ䖜儈㓴㜨㞪/փ∄ǃ㛮/փ∄儈Ҿሩ➗㓴ˈ䖜ѝ㓴㛍/փ儈Ҿ Ӣѝ㓴ˈᐞᔲᴹ㔏䇑ᆖѹ˄P<0.05˅DŽնк䘠ᐞᔲᰐࡲ䟿৽ᓄޣ㌫ˈᵚ㿱ަ⭏ 93 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ٬оሩ➗㓴∄䖳ˈᐞᔲ൷ᰐ㔏䇑ᆖ∄ᆖѹDŽަ։㓴ࣘ⢙㜿ಘ䟽䟿઼㜿/փ⢙ ѹ˄P<0.05˅DŽ⯵⨶㓴㓷ᆖỰḕᵚਁ⧠䈕䖜สഐṧ૱ᕅ䎧ᇎ傼ࣘ⢙ިර⯵⨶㓴 㓷ᆖ᭩ਈ઼⢩ᔲᙗᦏՔDŽ 㔬кˈ90 ཙབྷ啐ޘ伏૱ல侢䈅傼㺘᰾ˈ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ 侢ᯉ˄∄ֻѪ 50% ǃ25% ǃޕ᧪MON 87427 ઼䶎䖜สഐӢᵜ⦹㊣ EXP262 ࠶࡛ 12.5%˅ல侢བྷ啐 90 ཙˈᵚ㿱ަሩࣘ⢙ӗ⭏н㢟ᖡ૽DŽ 9˅MON 87427 ᶕⓀ CP4 EPSPS 㳻ⲭⲴӪ㗔㟣伏ᩴޕ仾䲙䇴ՠ ṩᦞ CP4 EPSPS 㳻ⲭⲴପңࣘ⢙ᙕᙗ∂ᙗᇎ傼㔃᷌ˈᶕ䇴ՠᲞ䙊Ӫ㗔ഐ㟣 伏⎸䍩 MON 87427 ⦹㊣㘼ᩴޕ CP4 EPSPS 㳻ⲭⲴ≤ᒣ઼᭸ᓄˈᱟᆹޘᙗ䇴ԧⲴ ањ䟽㾱ᯩ䶒DŽ䙊䗷ሿ啐ᙕᙗ∂ᙗᇎ傼ѝᰐ᰾ᱮᦏᇣ⭘ࡲ䟿˄NOAEL˅઼㟣 ᓧⲴ▌൘仾䲙DŽڕ᳤䵢䟿ਟԕ䇑㇇᳤ࠪ䵢䲀˄MOE˅ˈ䘋㘼䇴ՠሩӪ㊫ޕ伏ᩴ ᓧ⣦ߥⴁ⍻—2010-2013 ᒤ㔬ڕ⭡Ҿ 2015 ᒤছ䇑ငᯠਁᐳⲴljѝഭት≁㩕ޫо ਸᣕNJѝ⋑ᴹѝഭӪ㗔ሩ⦹㊣Ⲵ㟣伏ᩴޕ䟿Ⲵᯠᮠᦞˈሩ MON 87427 ᶕⓀ 仾䲙䇴ՠӽ❦֯⭘Ҷ㘏ࠔ㤡઼ᶘᲃݹ˄2006˅൘ޕCP4 EPSPS 㳻ⲭⲴӪ㗔㟣伏ᩴ ߥNJ⣦ޕᓧ⣦ߥᶑḕᣕѻҼ˄2002˅üü㟣伏о㩕ޫ㍐ᩴڕljѝഭት≁㩕ޫо ѝࡇࠪࠪѝഭӪᒣ൷⎸䍩⦹㊣Ⲵ䈳ḕᛵߥDŽṩᦞ䈕亩䈳ḕˈѝഭӪ⇿ཙ⇿Ӫᒣ൷ Ⲵ⦹㊣⎸䍩䟿Ѫ 9.9 gˈṩᦞѝഭӪᒣ൷փ䟽㓖 50 kgˈᣈ㇇ᡀ⇿ཙ⇿ޜᯔփ䟽⎸ 䍩䟿ࡉѪ 0.2 g˄㺘 21˅DŽ ൘䈕亩䈳ḕѝˈ⋑ᴹ⎹৺⇿Ӫ⇿ཙᴰབྷⲴ⦹㊣ᶕⓀ伏⢙˄वᤜ⦹㊣઼ਜ਼ᴹ⦹ Ҿᙕᙗ⎸䍩Ⲵՠ䇑ᱟᦞޣ䟿˄ᙕᙗ⎸䍩˅DŽഐ↔ˈᵜᣕѝޕ㊣Ⲵ伏૱˅Ⲵᩴ ц⭼ছ⭏㓴㓷ޘ⨳⧟ຳⴁ᧗㌫㔏 – 伏૱⊑ḃⴁ᧗৺䇴ՠ䇑ࡂ˄GEMS/Foodˈ www.who.int/foodsafety/chem/gems˅Ⲵ䈳ḕᮠᦞDŽGEMS/Food 亩ⴞᨀⲴᱟ њഭᇦᲞ䙊⎸䍩Ӫ㗔䙊䗷㟣伏⇿ཙᇎ䱵⎸䍩ᴰ儈ᩴޕ䟿Ⲵㅜ 97.5 Ⲯ࠶սⲴ⎸䍩 ⎸⢙㺘 21˅DŽᲞ䙊Ӫ㗔⦹㊣㊹Ⲵᙕᙗ⎸䍩٬ᱟ࠶࡛ṩᦞ⌅ഭ઼◣བྷ࡙ӊⲴ伏˄٬ ⢙䍩䈳ḕᗇࠪⲴDŽ㘼⭌⦹㊣઼⠶㊣㣡Ⲵᙕᙗ⎸䍩٬ࡉᱟ࠶࡛ṩᦞ⌠ഭ઼ᰕᵜⲴ伏 䍩䈳ḕᗇࠪⲴDŽ⭡Ҿ⋑ᴹањᚠᖃⲴᙫⲴㅜ 97.5 Ⲯ࠶սˈҏ⭡Ҿ䘉Ӌ⎸䍩٬⎸ ᶕⓀҾн਼ഭᇦ઼ൠ४ˈഐ↔ᗵ享ሩ䘉Ӌ伏⢙Ⲵᙕᙗ⎸䍩٬࠶࡛ՠ䇑DŽ ⭡Ҿ⦹㊣Ⲵѫ㾱⭘䙄ᱟѪ侢ᯉˈഐ↔ MON 87427 ⦹㊣৺ަ࣐ᐕⲴ伏૱ሶ ᶕਚՊঐѝഭӪ⦹㊣⎸䍩Ⲵаሿ䜘࠶DŽਖཆˈ䲔ⴤ᧕⎸䍩Ⲵ⠶㊣㣡઼ḀӋᖒᔿⲴ ⭌⦹㊣˄勌伏᷌ょ˅ཆˈབྷ䜭ᮠ⦹㊣䜭ᱟн਼⦹㊣ਸ൘а䎧Ⲵˈՠ䇑 MON 87427 ⎸ᇊѝഭӪٷᆸⲴᯩᔿ؍൘ਸ⦹㊣ѝⲴⲮ࠶ᮠ䶎ᑨഠ䳮DŽഐ↔ˈᡁԜਟԕ䟷ਆ 䍩Ⲵ⦹㊣ 100%䜭ᱟ MON 87427 ⦹㊣DŽ Ӿᆹޘ䇴ԧⲴ䀂ᓖᶕ䈤ˈਟԕ⭘ MON 87427 ㊭㋂ѝ CP4 EPSPS 㳻ⲭⲴᒣ൷ ≤ᒣᶕ䇑㇇⦹㊣㊹ǃ勌伏᷌ょ˄⭌⦹㊣˅઼⠶㊣㣡ѝ CP4 EPSPS 㳻ⲭⲴਜ਼䟿DŽ ṩᦞ 2008 ᒤ㖾ഭ⭠䰤䈅傼ѝਆṧ⍻ᇊⲴᮠᦞˈMON 87427 ㊭㋂Ⲵ CP4 EPSPS 㳻ⲭᒣ൷ਜ਼䟿Ѫ 3.6 g/g 勌䟽˄㿱 2.6.1 䜘࠶˅DŽᶕⓀҾ MON 87427 ⦹㊣㊹ǃ勌 伏᷌ょ˄⭌⦹㊣˅઼⠶㊣㣡Ⲵ CP4 EPSPS 㳻ⲭⲴᙕᙗ㟣伏ᩴޕ䟿䈖㿱㺘 22DŽ ሿ啐ᙕᙗ♼㛳ᇎ傼㔃᷌㺘᰾ CP4 EPSPS 㳻ⲭⲴᰐ᰾ᱮᦏᇣ⭘ࡲ䟿 ˄NOAEL˅Ѫ 572 mg/kg/ཙ˄㿱 3.3.1 䜘࠶˅DŽMOE ቡᱟṩᦞ䘉аࡲ䟿઼ 94 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 CP4 EPSPS 㳻ⲭⲴ㟣伏ᩴޕ䟿ᗇࠪⲴDŽ⭡㺘 22 ਟԕⴻࠪˈCP4 EPSPS 㳻ⲭⲴ ٬Ⲵ㤳ത൘ 1.4 × 104 㠣 7.9 × 105 ѻ䰤ˈᖃҾањփ䟽Ѫ 50 kg ⲴӪ䴰㾱 MOE ཙ⎸䍩㓖 7900 kg ˄䎵䗷㠚䓛փ䟽 150 ؽ˅Ⲵ MON 87427 ⦹㊣㊭㋂DŽ䘉Ӌᮠ⇿ ٬ᖃབྷˈӾਖаᯩ䶒䘋а↕䇱᰾൘ѝഭ䙊䗷⎸䍩 MON 87427 㘼᳤䵢Ҿ ᓧ仾䲙DŽᒦфˈṩᦞ 2016 ᒤᴰᯠⲴᙕᙗ∂⨶ᆖ⹄ウ㔃᷌ڕCP4 EPSPS нՊᑖᶕ ˄ᢰᵟᣕ15˅ˈCP4 EPSPS㳻ⲭ㓿ਓ♼㛳ሿ啐Ⲵ NOAEL㠣ቁѪ 5000 mg/kg bwˈ MOE Պᴤ儈DŽ ⭡↔ՠ㇇ᗇࠪⲴ CP4 EPSPS 㳻ⲭ᳤䵢䲀٬ 㺘21. ѝഭӪᒣ൷⦹㊣⎸䍩䟿1 ᙫӪਓ⦹㊣ᩴޕ≤ᒣ 伏⢙ ݻ/Ӫ/ཙ ݻ/ॳݻփ䟽/ཙ 2 ⦹㊣ 9.9 0.20 ˅ᓧ⣦ߥᶑḕᣕѻҼ˄2002˅NJ˄㘏ࠔ㤡, ᶘᲃݹ.2006ڕljѝഭት≁㩕ޫо 1 ”ṩᦞѝഭӪᒣ൷փ䟽Ѫ 50 kg ሶঅս“ݻ/Ӫ/ཙᦒ㇇Ѫ“ݻ/ॳݻփ䟽/ཙ 2 㺘22. ᶕⓀҾMON 87427Ⲵ伏⢙ѝCP4 EPSPS㳻ⲭⲴ㟣伏ᩴޕ᳤઼䵢䲀˄MOE˅ ⎸䍩⦹㊣ 1 CP4 EPSPS 㳻ⲭᩴޕ 2 ᳤䵢䲀˄MOE˅3 (g/kg/d) (μg/kg/d) สҾѝഭ䈳ḕᮠᦞ 伏⢙˖⦹⦹㊣ а㡜Ӫ㗔 0.20 0.70 7.9 × 105 ˄ᙫӪਓᒣ൷˅ สҾ GEMS/Food ᮠᦞ 伏⢙˖⦹⦹㊣㊹ а㡜Ӫ㗔 2.04 7.3 7.8 × 104 ˄ㅜ 97.5 Ⲯ࠶ս˅ 伏⢙˖⠶⠶㊣㣡 а㡜Ӫ㗔 3.33 12.0 4.8 × 104 ˄ㅜ 97.5 Ⲯ࠶ս˅ 伏⢙˖勌勌伏᷌ょ а㡜Ӫ㗔 7.16 25.8 2.2 × 104 ˄ㅜ 97.5 Ⲯ࠶ս˅ ˗ṩᦞ㘏ࠔ㤡઼ᶘᲃݹ˄2006˅ԕ৺ GEMS/Food ᮠᦞѝⲴᲞ䙊Ӫ㗔Ⲵ⦹㊣⎸䍩≤ᒣ 1 ᇊ⎸䍩Ⲵ⦹㊣ 100%䜭ᱟ MON 87427 ᒦф MON 87427 ㊭㋂ѝⲴ CP4 EPSPS 㳻ⲭٷ 2 ਜ਼䟿Ѫ 3.6 μg/g 勌䟽˄ppm˅˗ 3 ⭘ሿ啐♼㛳ᇎ傼ᗇࠪⲴ NOAEL˄572 mg/kg bw˅䲔ԕՠ㇇Ⲵ㳻ⲭᩴޕ䟿ᗇࡠ MOE ṩᦞ 2016 ᒤᴰᯠⲴᙕᙗ∂⨶ᆖ⹄ウ㔃᷌ˈCP4 EPSPS 㳻ⲭⲴ NOAEL 㠣ቁѪ 5000˗٬ MOE Պᴤ儈DŽ mg/kg bwˈ⭡↔ՠ㇇ᗇࠪⲴ CP4 EPSPS 㳻ⲭ᳤䵢䲀٬ 95 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.3.2 䗽ᮅᙝ ṩᦞഭ䱵伏૱⌅ިငઈՊ᧘㦀Ⲵᯠ㳻ⲭ▌൘㠤ᙗ䇴ԧᤷই˄Codex Alimentarius, 2003; Codex Alimentarius, 2009˅ˈḀ㳻ⲭⲴ▌൘㠤ᙗ䴰㾱਼ᰦ 䇴ԧ䈕㳻ⲭⲴ⭏ॆ⢩ᙗԕ৺оᐢ⸕㠤ⓀⲴᒿࡇլᙗDŽྲ᷌䈕㳻ⲭާᴹԕл⢩ ᖱˈࡉа㡜н䇔Ѫ䈕㳻ⲭҾ㠤㳻ⲭ˖ i) 䈕㳻ⲭᶕⓀҾ䶎㠤˗ ˗ii) 䈕㳻ⲭ൘ᙫ㳻ⲭਜ਼䟿ѝᡰঐ∄ֻᖸሿ iii) 䈕㳻ⲭⲴ≘ส䞨ᒿࡇоᐢ⸕㠤Ⲵ≘ส䞨ᒿࡇнާᴹᱮ㪇լᙗ˗ iv) 䈕㳻ⲭ൘ପңࣘ⢙㛳㛐⎸ॆ㌫㔏ѝ䗵䙏㻛⎸ॆ˗ v) 䈕㳻ⲭሩ✝༴⨶нっᇊˈ࣐✝ᶑԦл䗵䙏ཡ⍫DŽ ሩ MON 87427 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭⲴ▌൘㠤ᙗ䇴ՠҏᱟ䚥ᗚ䘉Ӌ ࡉˈ䇴ԧ㔃᷌䇔Ѫ CP4 EPSPS 㳻ⲭнާᴹ㠤ᙗDŽ 1˅CP4 EPSPS 㳻ⲭⲴ䮯ᵏᆹޘᓄ⭘শਢ ྲࡽ 3.3.1 䜘࠶᧿䘠ˈEPSPS 㳻ⲭᱟ㧭㥹䞨ԓ䉒䙄ᖴѝⲴањ䞦ˈ൘Ἵ⢙઼ ᗞ⭏⢙ѝᲞ䙽ᆈ൘ˈѪӪ㊫઼ࣘ⢙伏૱ѝⲴᡀ࠶ᐢ㓿ᓄ⭘Ҷᖃ䮯ᰦ䰤DŽ CP4 EPSPS 㳻ⲭᶕⓀҾ൏༔ߌᵶ㧼ˈҾ EPSPS 㳻ⲭᇦ᯿ⲴаDŽἽ⢙઼ ᗞ⭏⢙ѝⲴ EPSPS 㳻ⲭާᴹ儈ᓖⲴ㔃ᶴլᙗDŽ㺘䗮 CP4 EPSPS Ⲵߌ⢙ ˄བྷ䉶ǃ⦹㊣ǃỹ㣡ǃ⋩㨌ǃ㤌㬯઼⭌㨌ㅹ˅ᐢ㓿୶ъॆᓄ⭘䘁 20 ᒤDŽ㠣Ӻ⋑ ᴹԫօ、ᆖ䇱ᦞ㺘᰾ CP4 EPSPS 㳻ⲭՊ䙐ᡀӪ㊫䗷DŽ ᙗޘCP4 EPSPS 㳻ⲭփ⭏⢙Ⲵᆹ˅2 CP4 EPSPS 㳻ⲭⲴփ⭏⢙Ѫ൏༔ߌᵶ㧼 CP4 㧼ṚˈCP4 㧼Ṛਟԕ㺘䗮ཙ ❦Ⲵᒦфሩ㥹⭈㟖нᝏⲴ EPSPS 㳻ⲭˈᒦભѪ CP4 EPSPS 㳻ⲭ˄Padgette et al., 1996˅DŽ㖾ഭᗞ⭏⢙ษޫᓃ˄American Type Culture Collection˅ᐢ㓿ሩ䘉 CP4 㧼Ṛ䘋㹼Ҷ࠶઼䢤ᇊˈᴰ㓸⺞ᇊҾ൏༔ߌᵶ㧼Ⲵ⭏⨶ሿDŽⴞࡽ䘈⋑ᴹ ᣕ䚃㺘᰾ԫօаߌᵶ㧼ሩӪ㊫ᡆ㘵ࣘ⢙ާᴹ㠤⯵㜭࣋ˈ㘼фߌᵶ㧼а㡜нᱟ㠤 Ⓚ˄FAO/WHO, 1991˅DŽ↔ཆˈṩᦞ㚄ਸഭ㋞ߌ㓴㓷઼ц⭼ছ⭏㓴㓷˄FAO/WHO, 2001˅Ⲵᣕˈⴞࡽ䘈⋑ਁ⧠ԫօⲴӪ㗔ሩ㓶㧼ᙗ㳻ⲭ䗷DŽ 3˅CP4 EPSPS 㳻ⲭⲴ⭏ॆ⢩ᙗ઼㳻ⲭㅹ਼ᙗ࠶᷀ ྲࡽ3.3.1䜘࠶᧿䘠ˈCP4 EPSPSᱟ⭡455њ≘ส䞨㓴ᡀⲴঅ䬮ཊ㛭ˈ⨶䇪࠶ ᆀ䟿Ѫ47.6 kDaDŽⴞࡽᐢ⸕ⲴᡰᴹᶕⓀEPSPS䞦˄वᤜCP4 EPSPS˅൘й㓗ᤃᢁ 㔃ᶴк儈ᓖа㠤˄Hammond, et al. 2013˅DŽCP4 EPSPS䞦Ⲵ⤜⢩ᶴ䙐֯ᗇަሩ㥹 ᤱᖸ儈ⲴӢ઼ᙗDŽ؍⭈㟖нᝏˈն਼ᰦሩཙ❦ᓅ⢙PEP EPSPS 䞦˄वᤜ CP4 EPSPS˅ާᴹ儈ᓖⲴᓅ⢙⢩ᔲᙗDŽⴞࡽᐢ⸕ሩ EPSPS ਈॆDŽ׳䞦ާᴹ⭏⢙ᆖѹⲴᓅ⢙ӵѪ S3P ઼ PEPDŽ㥹⭈㟖н㜭ਁ⭏ EPSPS 䞦 ٬ǃᓖ઼ᆀᕪᓖㅹഐ㍐ᖡ૽DŽᴰ䘲 CP4 EPSPS 㳻ⲭⲴ䞦⍫ᙗ઼っᇊᙗਇ pH ٬䗷儈ᡆ䗷վ䜭Պ䱽վ䞦⍫ᙗˈpH ሿҾ 5.5 ᰦ CP4 EPSPS 䞦⍫ pH Ѫ 9-9.5ˈpH 96 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䞦⍫ࠐѾᆼޘїཡˈн䗷⭡ pH ᕅ䎧Ⲵ䞦⍫ਈॆᒦ䶎нਟ䘶˄Padgette et al. 1993˅DŽ CP4 EPSPS ሩ✝нっᇊˈᓖ䎵䗷 75°C ᰦˈCP4 EPSPS Ⲵ࣏㜭⍫ᙗণ䱽㠣Ự⍻ 䲀ԕлˈն࣐✝ᒦнᖡ૽㳻ⲭа㓗㔃ᶴⲴᆼᮤᙗDŽоᡰᴹިරⲴ㳻ⲭ䞦㊫аṧˈ CP4 EPSPS Ⲵۜॆ⍫ᙗ䘈ਇ৽ᓄⓦ⏢ѝᆀᕪᓖⲴᖡ૽DŽ↓ᑨⲴ䍞ᆈ㕃ߢ⏢˄1 mM KCl˅ᶑԦлˈCP4 EPSPS Ⲵ⢩ᔲ⍫ᙗᴰ儈㜭ཏ䗮ࡠ 5.5 U/mgDŽCP4 EPSPS Ⲵ⢩ᔲ⍫ᙗ䲿⵰ᆀᕪᓖ໎བྷ㘼ॷ儈ˈ㠣 50 mM KCl ᰦˈ䞦⍫ᙗ㜭ཏ໎࣐ 2-2.6 ؽ˄Padgette et al. 1993˅DŽ 䟷⭘а㌫ࡇ࠶᷀ᯩ⌅ሩ MON 87427 ⦹㊣㊭㋂ѝᨀਆ㓟ॆⲴ CP4 EPSPS 㳻ⲭ 䘋㹼Ҷ⢩ᖱ䢤ᇊˈᒦоབྷ㛐ᵶ㧼㺘䗮㌫㔏ѝ㓟ॆᗇࡠⲴ CP4 EPSPS 㳻ⲭ䘋㹼∄ 䖳DŽ࠶᷀ᯩ⌅वᤜ˖1˅⭘ Bio-Rad 㳻ⲭ࠶᷀㌫㔏⍻ᇊᙫ㳻ⲭ⎃ᓖ˗2˅ॱҼ✧ส ⼪䞨䫐-㚊щ✟䞠㜪ࠍ㜦⭥⌣˄SDS-PAGE˅৺ᇶᓖ䇑䟿˗3˅N-ᵛㄟᒿࡇ࠶᷀˗4˅ ส䍘䖵ࣙ◰ݹ䀓᷀⭥伎㹼ᰦ䰤˄MALDI-TOF˅䍘䉡˗5˅CP4 EPSPS 䞦⍫ᙗỰ ⍻˗6˅㌆สॆ࠶᷀˗7˅䍞ᆈっᇊᙗㅹ࠶᷀ˈᴰ㓸⺞ᇊ MON 87427 Ⲵ CP4 EPSPS 㳻ⲭоབྷ㛐ᵶ㧼⭏ӗⲴ CP4 EPSPS 㳻ⲭާᴹ਼Ⲵ⭏ॆ⢩ᙗˈҼ㘵ᇎ䍘ㅹ਼DŽ ᴹޣབྷ㛐ᵶ㧼㺘䗮Ⲵ CP4 EPSPS 㳻ⲭᐢᴹབྷ䟿Ⲵ⹄ウᣕ䚃ˈᡰᴹᇎ傼㔃᷌൷㺘 ᰾ᆳᱟᆹޘⲴˈнާᴹ㠤ᙗDŽ⭡↔ҏਟԕ᧘ᯝ MON 87427 Ⲵ CP4 EPSPS 㳻ⲭ ҏᱟᆹޘⲴDŽ ֻ∄CP4 EPSPS 㳻ⲭঐ㊭㋂ᙫ㳻ⲭⲴ˅4 ൘ MON 87427 ⦹㊣Ⲵ⭏䮯䗷〻ѝˈ䲔ᓄⲴ㣡㊹㓴㓷ཆˈަᆳн਼ಘᇈ㓴 㓷䜭㜭Ự⍻ࡠ CP4 EPSPS 㳻ⲭ˄㺘 9˅DŽ൘ᡰᴹ㓴㓷㊫රѝˈ㊭㋂ᱟӪ㊫઼ࣘ ⢙Ⲵ伏⭘䜘սˈഐ↔ᑨ⭘㊭㋂ᶕ䇴ԧަ㠤ᙗDŽ൘ MON 87427 ㊭㋂ѝⲴ CP4 EPSPS 㳻ⲭᒣ൷≤ᒣᱟ ˄㺘 9˅ˈ㘼㊭㋂ѝᒣ൷ᙫ㳻ⲭਜ਼䟿Ⲯ࠶ ᮠѪ㊭㋂ᒢ䟽Ⲵ 10.05%˄ᡆ㘵 100500 μg/g dwt˅DŽ䇑㇇ᗇࡠ CP4 EPSPS 㳻ⲭঐ ㊭㋂ᙫ㳻ⲭⲴ 0.004%˄ @ 100% = 0.004%˅DŽഐ↔ˈ CP4 EPSPS 㳻ⲭ൘㊭㋂ᙫ㳻ⲭѝⲴ∄ֻᶱվDŽ 5˅CP4 EPSPS оަᆳᐢ⸕㠤㳻ⲭⲴ㔃ᶴլᙗ ṩᦞ CODEX ᤷই˄Codex, 2003˅ˈྲ᷌Ḁ㳻ⲭоᐢ⸕㠤㳻ⲭ൘ԫօ 80 њԕк≘ส䞨⡷⇥Ⲵ਼≘ส䞨∄ֻ䗮ࡠ 35%ԕкˈࡉ㺘᰾Ҽ㘵ާᴹ㔃ᶴլ ᙗˈ䈕㳻ⲭਟ㜭ާᴹ▌൘㠤ᙗDŽCODEX ᤷই䘈᧘㦀䟷⭘䘎㔝━㹼デਓỰ㍒〻 ᒿࡔᯝḀ㳻ⲭᱟާᴹ▌൘㠤ᙗ˄Codex, 2003; Thomas et al., 2005˅DŽྲ᷌ⴞ Ⲵ㳻ⲭⲴ≘ส䞨ᒿࡇоḀ▌൘㠤ⓀⲴᣇփߣᇊ㈷ᴹ㠣ቁ 8 њ䘎㔝䛫≘ส䞨 ᱟ਼Ⲵˈ䛓Ѹ䈕≘ส䞨ਟԕ㻛䇔Ѫާᴹ▌൘Ⲵ㠤ᙗ˄Metcalfe et al., 1996; Hileman et al., 2002˅DŽ ࡙⭘ FASTA 䖟Ԧਟԕⴤ᧕∄ሩ≘ส䞨ᒿࡇ˄ণࡍ㓗㓯ᙗᒿࡇ˅ˈ∄ሩⲴ䱥ࡇ 㔃᷌ҏਟԕ⭘Ҿ᧘ᯝᴤ儈㓗Ⲵ㔃ᶴլᙗ˄ণ㳻ⲭⲴҼ㓗઼й㓗㔃ᶴ˅DŽ䙊ᑨ㳻 ⲭᒿࡇⲴլᙗ䎺儈ˈ਼Ⓚᙗҏቡ䎺儈DŽ㘼਼Ⓚ㳻ⲭᖰᖰާᴹޡ਼ⲴҼ㓗㔃ᶴ઼ й㔤㔃ᶴˈᒦާᴹ਼Ⲵ࣏㜭DŽ ⭘Ҿ CP4 EPSPS 㳻ⲭ㠤ᙗ࠶᷀ⲴᮠᦞᓃѪ AD_2016ˈवਜ਼ᴹ㠤㳻ⲭǃ 97 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䞷ⓦ㳻ⲭ઼哖䉧㳻ⲭ˄䈖㿱䲿⭣䈧ҖᨀӔⲴᢰᵟᣕ 6˅DŽAD_2016 ᮠᦞᓃवਜ਼ 1,956 ᶑᒿࡇ˄Hileman & Silvanovich, 2016˅ˈᶕⓀҾ伏⢙㠤⹄ウ઼䍴Ⓚ〻ᒿ ٬ᱟỰ㍒ѝሩ㿲ሏࡠ ᮠᦞᓃ FARRP˄2016˅˄http://www.allergenonline.org˅DŽE ٬䎺ሿˈ㺘᰾є㳻ⲭ-Ⲵլᙗᗇ࠶䲿ᵪਁ⭏ਟ㜭ᙗⲴ㔏䇑ᆖ䟿ᓖDŽ∄ሩⲴ E Ⲵᒿࡇլᙗቡ䎺儈DŽа㡜㘼䀰ˈᖃєњ㳻ⲭ൘ԫօ 80 њ≘ส䞨⡷⇥∄ሩⲴ 䶒ޘ٬䗮ࡠᡆվҾ 1×10-5ˈণਟԕ䇔Ѫ∄ሩⲴє㳻ⲭާᴹլᙗDŽѪҶᴤ-E E=0.01 䘋㹼⭏⢙ؑᆖ ᆸⲴ䰸٬؍ൠ䘋㹼䇴ԧˈӾ 2016 ᒤᔰˈ䟷⭘ањᶱㄟ ࠶᷀DŽ൘լᙗ∄ሩѝˈ䘈䟷⭘Ҷ䘎㔝 8 њ≘ส䞨━㹼デਓỰ㍒∄ሩˈԕ⺞ᇊ CP4 EPSPS оᐢ⸕㠤൘⸝Ⲵ㓯ᙗཊ㛭кᱟާᴹլᙗDŽ ⭏⢙ؑᆖ∄ሩ㔃᷌㺘᰾ˈCP4 EPSPS 㳻ⲭᒿࡇо AD_2016 ᮠᦞᓃѝⲴ㳻 ٬Ѫ 1.2˄ᢰ-ⲭᒿࡇ䰤լᙗ࠶᷀䇴࠶ᴰվⲴᱟ㕆ਧѪ GI-21725588 Ⲵ㳻ⲭˈަ E ؍٬䗷儈ˈᴰ儈ᗇ࠶઼㿲ሏࡠⲴަԆ∄ሩ㔃᷌н㜭㺘᰾ᆈ൘-ᵟᣕ 6˅DŽ⭡Ҿ E ᆸᒿࡇᡆ▌൘Ⲵ㠤Ӕ৹⍫ᙗˈ∄ሩᰐᴹ᭸ѹDŽഐ↔ᡰᴹ∄ሩ㔃᷌ᵚ䗮ࡠᡆ䎵 䗷伏૱⌅ިငઈՊ˄Codex, 2003˅᧘㦀Ⲵ 80 њ≘ส䞨↻สᴹ 35%≘ส䞨լ ٬ሿҾᡆㅹҾ 1×10-5˅DŽ↔ཆˈCP4 EPSPS 㳻ⲭᒿࡇо AD_2016-Ⲵ䰸٬ḷ߶˄E ᒿࡇᮠᦞᓃѝ㳻ⲭ䘋㹼∄䖳ᰦˈҏᵚ൘ AD_2016 ᮠᦞᓃѝᩌ㍒ࡠᴹ䘎㔝 8 њ≘ ส䞨਼Ⲵ㳻ⲭDŽᙫѻˈ⭏⢙ؑᆖ࠶᷀䇱᰾ˈCP4 EPSPS 㳻ⲭоᐢ⸕Ⲵ㠤 Ⲵᒿࡇ઼㔃ᶴкⲴլᙗDŽޣнާᴹݽ⯛ᆖ 6˅CP4 EPSPS 㳻ⲭփཆ⁑ᤏ⎸ॆᇎ傼 Harrison ㅹ˄ 1996˅䇱᰾Ҷ CP4 EPSPS 㳻ⲭ㜭൘⁑ᤏ⎸ॆᶑԦлՊ㻛䗵䙏⎸ ॆᦹDŽṩᦞ Western ঠ䘩࠶᷀ˈCP4 EPSPS ൘⁑ᤏ㛳⏢⎸ॆ㌫㔏ѝ 15 s ਾቡᰐ⌅ Ự⍻ࡠDŽ ᆏኡ䜭ޜਨሩ CP4 EPSPS 㳻ⲭ൘փ⁑ᤏ㛳⏢˄SGF˅⎸ॆᇎ傼ҏ䇱ᇎҶ к䘠㿲⛩DŽ䈕䈅傼䟷⭘ഭ䱵⭏ભ、ᆖ⹄ウᡰ˄ILSI˅ޜᐳⲴḷ߶ᯩ⌅˄Thomas et al., 2005˅DŽ਼ Harrison ㅹ˄1996˅ԕࡽⲴᇎ傼аṧˈᵜ亩⹄ウҏ䟷⭘Ҷབྷ㛐ᵶ㧼 ⭏ӗⲴ CP4 EPSPS 㳻ⲭˈ⎸ॆ⦷䟷⭘ SDS-PAGE ⭥⌣ࠍ㜦ḃ㢢ǃ㳻ⲭ䍘ঠ䘩઼ CP4 EPSPS 䞦৽ᓄ⍫ᙗ䢤ᇊ䘋㹼䇴ՠDŽ ᇎ傼㔃᷌䇱ᇎҶ CP4 EPSPS 㳻ⲭ൘փཆ⁑ᤏⲴ SGF 䟼䗵䙏㻛⎸ॆDŽ SDS-PAGE ࠍ㜦ḃ㢢㔃᷌㺘᰾ˈ98%ԕкⲴ CP4 EPSPS 㳻ⲭ൘ 15 s 㻛䗵䙏⎸ ॆ˄മ 21˅DŽWestern ᵲӔ࠶᷀㔃᷌㺘᰾ˈ95%ԕкⲴ CP4 EPSPS 㳻ⲭ൘ 15 s ণᰐ⌅Ự⍻˄മ 22˅DŽ਼ṧˈCP4 EPSPS 㳻ⲭ൘ SGF 䟼ษޫнࡠ 15 sˈަ⍫ᙗ䱽 վࡠ 10%ԕл˄㺘 18˅DŽᙫѻˈփཆ⁑ᤏ⎸ॆᇎ傼Ⲵ㔃᷌ਟԕᯝᇊ CP4 EPSPS ᓧӗ⭏ড䲙DŽڕ㳻ⲭ㜭൘⁑ᤏ㛳⏢䟼䗵䙏㻛䱽䀓ˈᡰԕнਟ㜭ሩӪփ 7˅CP4 EPSPS 㳻ⲭⲴ✝っᇊᙗᇎ傼 ࣐✝༴⨶൘⦹㊣㊭㋂ԕ৺ਜ਼ᴹ⦹㊣㊭㋂Ⲵӗ૱࣐ᐕ䗷〻ѝᓄ⭘䶎ᑨᒯ⌋ˈഐ ↔䇴ՠ CP4 EPSPS 㳻ⲭሩ✝༴⨶Ⲵっᇊᙗҏᱟ䶎ᑨ䟽㾱ⲴDŽ ✝༴⨶ᇎ傼⭘Ⲵ CP4 EPSPS ᱟ⭡བྷ㛐ᵶ㧼⭏ӗⲴˈ✝༴⨶ᓖѪ 25°Cǃ37°Cǃ 55°Cǃ75°C ઼ 95°Cˈ༴⨶ᰦ䰤Ѫ 15 min ᡆ㘵 30 minDŽ䇴ԧⲴᇩवᤜ˖1˅࣐ 98 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ✝༴⨶ሩ CP4 EPSPS 㳻ⲭ䞦⍫࣋Ⲵᖡ૽˗2˅࣐✝༴⨶ሩ㳻ⲭᆼᮤᙗⲴᖡ૽DŽ ൘ 25°Cǃ37°Cǃ55°Cǃ75°C ઼ 95°C ᓖᶑԦл࣐✝༴⨶ 15 ઼ 30 min ਾˈ CP4 EPSPS 㳻ⲭⲴ⍫ᙗ⍻䈅㔃᷌࠶࡛㿱㺘 19 ઼㺘 20DŽᖃԕ 25ºCǃ37ºC ઼ 55ºC ࣐✝ 15 min ਾ㿲ሏࡠ CP4 EPSPS ⍫ᙗ〽ᴹ䱽վˈ࠶࡛䱽㠣ሩ➗Ⲵ 81%ǃ84%઼ 70%˗ն࣐✝ 30 min ਾˈ25ºC ༴⨶Ⲵ CP4 EPSPS ⍫ᙗ⋑ᴹਈॆˈ37ºC ༴⨶㓴Ⲵ 䞦⍫䱽㠣ሩ➗ⲴⲴ 88%ˈ㘼 55ºC ༴⨶㓴Ⲵ CP4 EPSPS 㺘⧠Ѫབྷᑵл䱽ˈӵѪሩ ➗Ⲵ 25%DŽн䇪ᱟ༴⨶ 15 min 䘈ᱟ༴⨶ 30 minˈ75°C ઼ 95°C ᓖᶑԦлⲴ CP4 EPSPS 䞦⍫൷վҾỰ⍻䲀DŽ SDS-PAGE ࠶᷀㔃᷌㺘᰾ˈ৲➗ḷ߶઼ሩ➗⢙वਜ਼аᶑ㺘㿲࠶ᆀ䟿㓖Ѫ 43.8 kDa ⲴѫᶑᑖˈሩᓄҾ CP4 EPSPS 㳻ⲭ˄മ 23 ઼മ 24˅DŽ൘ᡰᴹ༴⨶ᰦ䰤 ⌣ˈ༴⨶ᓖᶑԦлˈṧ૱Ⲵ 43.8 kDa ᶑᑖؑਧᕪᓖ⋑ᴹ᰾ᱮⲴ䱽վ˄മ 23઼ 䚃 3~7˗മ 24ˈ⌣䚃 2~7˅DŽ к䘠㔃᷌㺘᰾ˈ࣐✝༴⨶ӵՊ֯ CP4 EPSPS 㳻ⲭⲴ䞦⍫ᙗ䱽վˈնнՊ֯ CP4 EPSPS 㳻ⲭ䱽䀓DŽഐ↔ˈнՊᖒᡀᯠⲴ㳻ⲭ⡷⇥㘼ᑖᶕᯠⲴ▌൘㠤仾䲙DŽ 8˅ਇփⲴ㠤ᙗ࠶᷀ ྲㅜ 1.2.3 䜘࠶ѝᡰ䘠ˈ⦹㊣䮯ᵏѪӪ㊫઼ࣘ⢙Ⲵ伏⢙ˈ䙊ᑨҾ䶎䗷 ᙗ伏⢙DŽⴞࡽᖸቁᴹޣ伏⭘⦹㊣ӗ૱ਾਁ⭏䗷৽ᓄⲴᣕ䚃˄OECD, 2002˅DŽ ,.ᣕ䚃˄Pauls and Cross, 1998; Tanaka et alޣ䗷৫ॱࠐᒤѝˈ൘㖾ഭਚᴹєֻ 2001˅DŽJones et al.˄1995˅䇔Ѫˈ䇨ཊሩ㊭㋂㺘⧠䗷ⲴӪᇎ䱵кᱟሩ㣡㊹䗷 ˈ䘉ӋӪѝᴹབྷ㓖 80%ⲴӪ䘋㹼伏⢙᭫ࠫ䈅傼ᰦ⋑ᴹӗ⭏Ѥᒺ䗷⯷⣦DŽ↔ ཆˈ൘ᴮ㓿ሩ㊭㋂ӗ⭏䗷৽ᓄⲴӪѝˈ㲭❦ཊᮠՊሩሿ哖㳻ⲭ䍘ਁ⭏䗷৽ ᓄ˄~75%˅ˈնሩ⦹㊣㊭㋂ӗ⭏䗷৽ᓄⲴӪᶱቁˈаⲮཊս⯵Ӫѝнࡠ 6 ս 䈕ሿݯ、䗷৽ᓄу、ѝᗳᣕਁ⭏Ҷ䗷৽ᓄDŽੁ ᶕ㠚Ҿབྷ࡙Ⲵєԭᣕ˄Pastorello et al., 2000; Pasini et al., 2002˅㺘᰾ˈ ᴹаӋ䗷⯵Ӫࠪ⧠Ⲵ伏૱䗷৽ᓄˈ਼伏⭘वᤜ⦹㊣㋕൘Ⲵ⦹㊣ӗ૱ࠪ⧠ Ⲵ䗷৽ᓄ⯷⣦а㠤ˈަѝᶕ㠚⌒䟼Ⲵ 6 ս⯵Ӫ䖫ᓖ伏૱᭫ࠫ䈅傼㔃᷌ 䱣ᙗDŽնᱟˈк䘠བྷ࡙⹄ウѝᡰᣕⲴ⯵Ӫˈ䙊ᑨሩᵲ㥹ǃprunidae ㊫Ἵ⢙ ӗ⭏Ⲵ㣡㊹ǃ㊭㋂৺俉ᯉㅹˈ䜭Պਁ⭏ཊ䟽䗷৽ᓄDŽཊ䟽䗷৽ᓄ֯ᗇѤᒺ к䇶࡛⢩ᇊ⯵ֻⲴ㠤ᰦާᴹн⺞ᇊᙗ˗⭡Ҿ䗷৽ᓄѻ䰤ᡆཊᡆቁ▌൘Ⲵ Ӕ৹⭘ˈ֯ᗇⳞ䈅䇺ᯝ઼փ IgE 㔃ਸ䈅傼ᴤѪ༽ᵲ˄Pastorello et al., 2000˅DŽ ⦹㊣㊭㋂ѝѫ㾱㠤ѻаѪ࠶ᆀ䟿 9kDaⲴ㜲䖜䘀㳻ⲭ˄LTPˈlipid transfer protein˅ˈҾ伏⢙ѫ㾱䗷ॱޛ䟋ӊᇦ᯿ˈо≤にǃṳᆀоབྷ哖ㅹ䗷਼ Ⓚˈ⋑ᴹਁ⧠⦹㊣⢩ᔲⲴ LTP 䗷˄Pastorello et al.ˈ2000˅DŽ⦹㊣оަԆἽ ⢙㣡㊹䗷ޣᡀԭሩ∄࠶᷀⋑ᴹਁ⧠⦹㊣⢩ᔲⲴ㠤˄Petersen A., 2006˅DŽⴞࡽˈ䘈⋑ᴹ䏣ཏⲴ䇱ᦞᶕҶ䀓൘བྷ࡙ᡆൠѝ⎧ൠ४⦹㊣ӗ૱䗷৽ ᓄ⍱㹼Ⲵ⢩⛩ˈҏ䘈⋑ᴹᶕ㠚Ҿц⭼ަԆൠ४ޣҾ⦹㊣伏૱䗷৽ᓄⲴᣕᇎ DŽ䘉㺘᰾ˈ⭡Ҿ伏⭘⦹㊣৺ަ㹽⭏ӗ૱ˈሩӪ㊫䙐ᡀ䗷৽ᓄ仾䲙Ⲵਟ㜭ᙗֻ ᖸվ˄Scibilia et al., 2008˅DŽ 99 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.3.3 ᣍ㩛ޱഖᆆ ṩᦞ OECD˄OECDˈ2002˅Ⲵᣕ䚃ˈ⦹㊣㊭㋂ਜ਼ᴹєѫ㾱Ⲵᣇ㩕ޫᡀ ࠶ˈণἽ䞨઼ỹᆀ㌆DŽἽ䞨ᆈ൘Ҿ⦹㊣㊭㋂ѝˈ㜭ཏо䫉ǃ䭱ǃ䫮ǃ䫱ਸ䬼ㅹ ⸯ⢙㩕ޫ㷟ਸˈ֯ᗇঅ㛳ࣘ⢙н㜭࡙⭘㊭㋂ѝⲴ䘉Ӌݳ㍐˄Lienerˈ2000˅DŽ ỹᆀ㌆ᱟᆈ൘Ҿ⦹㊣㊭㋂ѝⲴа࠶ᆀ䟿䖳վфнਟ㻛⎸ॆⲴ⻣≤ॆਸ⢙ˈ⭡ Ҿަӗ≄ਟሬ㠤㛳㛰ˈᡰԕҏ㻛䇔Ѫᱟаᣇ㩕ޫഐᆀ˄Lienerˈ2000˅DŽ Ӿ 2008 ᒤ㖾ഭⲴ⭠䰤䈅傼ѝ 3 њൠ⛩˄⡡㦧ॾᐎⲴ Jefferson 䜑ǃԺ࡙䈪 ԺᐎⲴ Stark 䜑઼䱯㛟㢢ᐎⲴ Jackson 䜑˅ਆṧ䘋㹼ᡀ࠶࠶᷀DŽ⭠䰤䈅傼䟷⭘ᆼ ޘ䲿ᵪ४㓴䇮䇑ˈ3 ⅑䟽༽DŽਆṧᶀᯉѝ䘈वᤜ 4 њᐢ㓿୶ъॆἽⲴᑨ㿴⦹ ㊣ᵲӔDŽਆṧࡽሩ MON 87427 ௧ᯭ㥹⭈㟖˄௧ᯭࡲ䟿Ѫ 1.12 kg ai/ha˅DŽ ṩᦞᡰᴹ䈅傼⛩Ⲵᒣ൷㔃᷌˄㺘 23˅ˈ㥹⭈㟖༴⨶Ⲵ MON 87427 оᑨ㿴 ሩ➗ѻ䰤Ἵ䞨Ⲵᒣ൷٬ᆈ൘㔏䇑ᆖᱮ㪇ᙗᐞᔲ˄p<0.05˅ˈնҼ㘵䰤൘ỹᆀ㌆ ਜ਼䟿ᯩ䶒ࡉ⋑ᴹᱮ㪇ᙗᐞᔲDŽሩ MON 87427 ઼ሩ➗䰤Ἵ䞨ਜ਼䟿Ⲵᱮ㪇ᐞᔲ䘋 㹼䘋а↕Ⲵ࠶᷀ˈਟԕⴻࠪ䘉ᱮ㪇ᙗᐞᔲнާᴹ⭏⢙ᆖѹDŽ俆ݸˈ㿲ሏࡠ Ⲵᱮ㪇ᙗᐞᔲᖸሿˈMON 87427 ⲴἽ䞨ਜ਼䟿∄ሩ➗վ 5.92%DŽަ⅑ˈMON 87427 Ἵ䞨ਜ਼䟿Ⲵᒣ൷٬൘୶ъॆ৲➗૱ᒣ൷٬Ⲵ 99%ᇩ䇨४䰤DŽㅜйˈ൘њ 䈅傼⛩Ⲵঅ⤜࠶᷀ᰦᒦ⋑ᴹ㿲ሏࡠ MON 87427 ઼ሩ➗䰤ⲴἽ䞨ਜ਼䟿ᴹ㔏䇑ᆖ ᱮ㪇ᙗᐞᔲDŽㅜഋˈMON 87427 Ἵ䞨ᒣ൷٬㩭൘ ILSI Ⲵ⢙㩕ޫᡀ࠶ᮠᦞᓃ ˄ILSIˈ2006˅ѝ⦹㊣Ἵ䞨ਜ਼䟿㠚❦ਈᔲ㤳തDŽ ṩᦞ䘉Ӌ㔃᷌ˈӾ伏૱઼侢ᯉᆹޘᡆ㩕ޫ䀂ᓖᶕⴻˈ㥹⭈㟖༴⨶Ⲵ MON 87427 оᑨ㿴ሩ➗ѻ䰤൘ᣇ㩕ޫᡀ࠶ᯩ䶒ᱟᇎ䍘ㅹ਼ⲴDŽ 3.3.4 㩛ޱᡆ࠼ MON 87427 Ⲵᡀ࠶࠶᷀㔃᷌ԕ৺оᑨ㿴ሩ➗઼୶ъॆ৲➗૱Ⲵᡀ࠶∄ 䖳㔃᷌㺘᰾ˈMON 87427 ઼ᑨ㿴⦹㊣൘㓴ᡀᡀ࠶кᇎ䍘ㅹ਼DŽ ᡰᴹ⭘Ҿᡀ࠶࠶᷀Ⲵṧ૱ᶀᯉᱟӾ 2008 ᒤ㖾ഭ⭠䰤䈅傼ѝ䟷䳶ⲴDŽ䟷ṧ Ⲵ䈅傼ൠ⛩Ѫ 3 њˈ࠶࡛Ѫ⡡㦧ॾᐎⲴ Jefferson 䜑ǃԺ࡙䈪ԺᐎⲴ Stark 䜑઼ 䱯㛟㢢ᐎⲴ Jackson 䜑DŽ⭠䰤䈅傼䟷⭘ᆼޘ䲿ᵪ४㓴䇮䇑ˈ࠶࡛䇮 3 ⅑䟽༽DŽ ਆṧᶀᯉѝ䘈वᤜ਼ᰦ䘋㹼⭠䰤䈅傼Ⲵ 4 њᐢ㓿୶ъॆἽⲴᑨ㿴⦹㊣ᵲӔDŽ ਆṧࡽሩ MON 87427 ௧ᯭ㥹⭈㟖䘋㹼༴⨶˄⎃ᓖѪ 1.12 kg ai/ha˅DŽ ࠶᷀Ⲵᡀ࠶वᤜ㩕ޫᡀ࠶ǃᣇ㩕ޫᡀ࠶˄ᐢ൘ 3.3.3 䜘࠶ӻ㓽˅઼⅑⭏ԓ 䉒⢙䍘DŽOECD ޡ䇶᮷Ԧ˄OECD, 2002˅ᐢሩ⦹㊣Ⲵᡀ࠶䘋Ҷ䈖㓶᧿䘠DŽ㩕ޫ ᡀ࠶वᤜ˖㊭㋂ѝⲴᑨ㿴ᡀ࠶˄⚠࠶ǃᣈ㇇Ⲵ⻣≤ॆਸ⢙ǃ≤࠶ǃ㳻ⲭ䍘઼㜲 㛚˅ǃ䞨ᙗ⍇⏔㓔㔤˄ADF˅ǃѝᙗ⍇⏔㓔㔤˄NDF˅ǃᙫ㟣伏㓔㔤˄TDF˅ǃ≘ ส䞨ǃ㜲㛚䞨˄C8~C22˅ǃⸯ⢙䍘˄䫉ǃ䬌ǃ䫱ǃ䭱ǃ䭠ǃ⼧ǃ䫮ǃ䫐઼䬼˅ ઼㔤⭏㍐˄ਦ䞨ǃ✏䞨ǃ㔤⭏㍐ A˄!㜑㩍ঌ㍐˅ǃ㔤⭏㍐ B1ǃ㔤⭏㍐ B2ǃ㔤 ⭏㍐ B6 ઼㔤⭏㍐ E˅ԕ৺ൠк䜘ἽṚⲴᑨ㿴ᡀ࠶ǃADFǃNDFǃ䫉઼⼧DŽᣇ㩕 100 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ޫᡀ࠶वᤜἽ䞨઼ỹᆀ㌆DŽ⅑⭏ԓ䉒ӗ⢙वᤜ㌐䟋ǃ䱯兿䞨઼ሩ-俉䉶䞨DŽഐ↔ˈ ᙫޡ࠶᷀Ҷ 78 ᡀ࠶˄वਜ਼ᣇ㩕ޫᡀ࠶઼⅑⭏ԓ䉒ӗ⢙˅ˈަѝ㊭㋂ᡀ࠶ 69 ˈൠк䜘ἽṚᡀ࠶ 9 DŽᙫޡ࠶᷀Ⲵ 78 ᡀ࠶ѝˈᴹ 16 ㊭㋂ᡀ࠶˄15 㩕ޫᡀ࠶઼ 1 ᣇ㩕ޫᡀ࠶˅䎵䗷 50%Ⲵ࠶᷀⋑ᴹ䗮ࡠᇊ䟿л䲀˄LOQ˅ˈᰐ ⌅䘋㹼㔏䇑࠶᷀DŽഐ↔ਚሩަᆳᡀ࠶䘋㹼Ҷ㔏䇑࠶᷀DŽሶᡀ࠶ਜ਼䟿ᣈ㇇Ѫᒢส ਜ਼䟿ਾ䘋㹼㔏䇑࠶᷀ˈਚᴹ≤࠶˄⒯สਜ਼䟿˅઼㜲㛚䞨ਜ਼䟿˄ঐᙫ㜲㛚䞨%˅ ཆDŽֻ ⇿ᡀ࠶൷वᤜ 4 њ㔏䇑ሩ∄ˈণ 3 њ䈅傼⛩࠶࡛ሩ∄઼ᡰᴹ䈅傼⛩ᒣ൷ ٬ሩ∄DŽ㔏䇑ᐞᔲⲴᱮ㪇ᙗ≤ᒣѪ 5%˄ ` ˅DŽ4 њ୶ъॆ৲➗૱Ⲵᡰᴹ 䈅傼⛩Ⲵᡀ࠶ᒣ൷٬⭘ᶕ⺞ᇊ䈕ᡀ࠶Ⲵ 99%ᇩ䇨४䰤DŽILSI ⢙㩕ޫᡀ࠶ᮠᦞ ᓃѝ⦹㊣Ⲵᡀ࠶ਜ਼䟿Ѫ⦹㊣ᡀ࠶㠚❦ਈᔲⲴ㤳തDŽ MON 87427 ⦹㊣㊭㋂㩕ޫᡀ࠶˖ ൘࠶᷀Ⲵ 69 ㊭㋂ᡀ࠶ѝˈᴹ 64 Ѫ㩕ޫᡀ࠶ˈवᤜᑨ㿴ᡀ࠶˄5 ˅ǃ ADFǃNDFǃTDFǃ≘ส䞨˄18 ˅ǃ㜲㛚䞨˄22 ˅ǃ㔤⭏㍐˄7 ˅઼ⸯ⢙ ˈ䍘˄9 ˅DŽ࠶᷀䗷〻ѝDŽᴹ 15 ㊭㋂㩕ޫᡀ࠶䎵䗷 50%Ⲵ࠶᷀٬վҾ LOQ ഐ↔⋑ᴹ㓣ޕᴰਾⲴ㔏䇑࠶᷀DŽ൘䘋㹼㔏䇑࠶᷀Ⲵ 49 㩕ޫᡀ࠶ѝ˄㺘 24˅ˈ ᴹ 43 ᡀ࠶Ⲵᒣ൷٬൘ MON 87427 ઼ᑨ㿴ሩ➗䰤нᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲDŽ MON 87427 ઼ᑨ㿴ሩ➗ᒣ൷٬ᆈ൘ᱮ㪇ᙗᐞᔲⲴᡀ࠶ᴹ 6 ˈ࠶࡛Ѫ 16:0 ἅ Ᾰ䞨ǃ18:0 ⺜㜲䞨ǃ18:1 ⋩䞨ǃ18:2 ӊ⋩䞨ǃ20:0 㣡⭏䞨઼ᙫ㜲㛚䞨˄㺘 27˅DŽ ሩާᴹᱮ㪇ᐞᔲⲴ 6 ㊭㋂㩕ޫᡀ࠶䘋㹼䘋а↕Ⲵ䇴ԧˈਟԕᗇࠪ㔃䇪˖ ൘ާᴹ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴ㩕ޫᡀ࠶ሩ∄ѝˈMON 87427 Ⲵᒣ൷٬о˅1 ᑨ㿴ሩ➗Ⲵᒣ൷٬ᐞᔲᖸሿ˄н䇪ᱟ儈Ҿሩ➗䘈ᱟվҾሩ➗˅ˈণҼ㘵 䰤ᐞᔲབྷሿ㤳തӵᖃҾሩ➗ᒣ൷٬Ⲵ 1.96%~5.09%˄ާփᶕ䈤ˈ16:0 ἅᾸ䞨Ѫ儈 3.52%ǃ18:0 ⺜㜲䞨儈 3.67%ǃ18:1 ⋩䞨儈 3.22%ǃ18:2 ӊ ⋩䞨վ 1.96%ǃ20:0 㣡⭏䞨儈 4.00%ǃᙫ㜲㛚ਜ਼䟿վ 5.09%˅DŽ ሩҾ䘉Ӌާᴹ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶ˈMON 87427 Ⲵᒣ൷٬൷൘˅2 ᦞ୶ъॆ૱Ⲵᒣ൷٬ᔪ・䎧ᶕⲴ 99%ᇩ䇨४䰤˄㺘 24 ઼㺘 27˅DŽ 䘉ቡણ⵰ MON 87427 Ⲵᒣ൷٬༴൘୶ъॆ⦹㊣૱㠚❦ਈᔲ㤳ത DŽ 3˅ྲ᷌䘋а↕㘳㲁䘉Ӌާᴹ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶൘н਼ൠ⛩Ⲵ㺘⧠ ਟԕⴻࠪˈMON 87427 ઼ሩ➗䰤 18:0 ⺜㜲䞨઼ 20:0 㣡⭏䞨Ⲵᱮ㪇ᙗ ᐞᔲਚ൘ 1 њൠ⛩ࠪ⧠ˈ㘼൘ަᆳ 2 њൠ⛩⋑ᴹࠪ⧠˗16:0 ἅᾸ䞨ǃ 18:1 ⋩䞨઼ 18:2 ӊ⋩䞨Ⲵ㔏䇑ᆖᱮ㪇ᙗᐞᔲࡉ൘ 3 њൠ⛩ࠪ⧠˄㺘 27˅DŽ 㘼ሩҾᙫ㜲㛚ਜ਼䟿Ⲵᱮ㪇ᙗᐞᔲӵࠪ⧠൘ᡰᴹ䈅傼⛩Ⲵᒣ൷ᮠ∄䖳кˈ ൘ԫօањঅњൠ⛩ˈMON 87427 ઼ሩ➗൷⋑ᴹᱮ㪇ᙗᐞᔲDŽնн㇑ ᘾṧˈMON 87427 Ⲵ䘉Ӌᡀ࠶ᒣ൷٬൷൘୶ъॆ૱ᒣ൷٬Ⲵ 99%ᇩ 䇨४䰤DŽ 101 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 4˅ሩҾ䘉Ӌ㺘⧠ࠪ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶ˈн䇪ᱟ MON 87427 䘈ᱟሩ ➗ˈᡀ࠶ᒣ൷٬൷൘᮷⥞ਁ㺘Ⲵ⦹㊣㊭㋂㩕ޫᡀ࠶Ⲵ㤳തˈҏ൘ ISLI ⢙㩕ޫᮠᦞᓃ˄ILSI, 2006˅ޣ⦹㊣㊭㋂㩕ޫᡀ࠶㤳ത˄㺘 28˅DŽ ᙫѻˈ൘䘋㹼㔏䇑࠶᷀Ⲵ 49 ㊭㋂㩕ޫᡀ࠶ѝˈᴹ 43 ᡀ࠶ MON 87427 ઼ᑨ㿴ሩ➗䰤нᆈ൘㔏䇑ᆖᱮ㪇ᙗᐞᔲˈᴹ 6 ᡀ࠶ᆈ൘ᱮ㪇ᐞᔲDŽ൘䘉Ӌާ ᴹ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶ᯩ䶒ˈMON 87427 ઼ᑨ㿴ሩ➗൘ 16:0 ἅᾸ䞨ǃ18:1 ⋩䞨઼ 18:2 ӊ⋩䞨ᯩ䶒Ⲵᱮ㪇ᐞᔲ൘н਼䈅傼ൠ⛩㺘⧠䖳Ѫа㠤ˈ㘼Ҽ㘵൘ަ ᆳᡀ࠶к˄18:0 ⺜㜲䞨ǃ20:0 㣡⭏䞨઼ᙫ㜲㛚ਜ਼䟿˅ᱮ㪇ᐞᔲ൘н਼ൠ⛩䰤㺘 ⧠на㠤˗նӾᮠ䟿кⴻˈҼ㘵൘ 6 ᡀ࠶䰤ᆈ൘ᱮ㪇ᙗᐞᔲᖸሿ˗ሩҾᡰᴹ 㺘⧠ࠪᱮ㪇ᙗᐞᔲⲴᡀ࠶ˈMON 87427 Ⲵᒣ൷٬൷㩭൘ᐢਁ㺘᮷⥞઼ ILSI ⢙㩕ޫᮠᦞᓃⲴ⦹㊣㊭㋂㩕ޫᡀ࠶Ⲵ↓ᑨ㤳തDŽഐ↔ˈਟԕᗇࠪ MON 87427 ઼ᑨ㿴ሩ➗൘㊭㋂㩕ޫᡀ࠶ᯩ䶒ᇎ䍘ㅹ਼Ⲵ㔃䇪DŽ MON 87427 ㊭㋂⅑⭏ԓ䉒ӗ⢙˖ ṩᦞ OECD˄2002˅DŽ⦹㊣㊭㋂ਜ਼ᴹ 3 ѫ㾱Ⲵ⅑⭏ԓ䉒ӗ⢙˖㌐䟋ǃ䱯 兿䞨઼ሩ-俉䉶䞨DŽ䶎⏰㊹ཊ㚊ᠺ㌆ᱟᱟ㌐䟋Ⲵѫ㾱ᶕⓀ˄Adams et al., 1997˅DŽ 䱯兿䞨઼ሩ-俉䉶䞨ࡉ⭡㣣俉᯿≘ส䞨˄㤟щ≘䞨઼䞚≘䞨˅ԓ䉒ӗ⭏ˈᱟ㤟ส щ䞨㊫ॆਸ⢙Ⲵࡽփ˄Buchanan et al., 2000˅DŽ MON 87427 ઼ሩ➗㊭㋂ѝ㌐䟋ⲴỰ⍻൷ሿҾᇊ䟿Ự⍻䲀˄LOQ ˈ ppm˅ˈᵚ䘋㹼㔏䇑࠶᷀DŽMON 87427 Ⲵ䱯兿䞨઼ሩ-俉䉶䞨ᒣ൷٬оᑨ 0.500> ᴹ㔏䇑ᆖᱮ㪇ᙗᐞᔲ˄ ` ˅˄㺘 25˅DŽ⋐㿴ሩ➗઼୶ъॆ৲➗૱Ⲵᒣ൷٬ ഐ↔ˈӾ⅑⭏ԓ䉒ӗ⢙ᶕⴻˈMON 87427 ઼ᑨ㿴ሩ➗ᇎ䍘ㅹ਼DŽ MON 87427 ൠк䜘ᮤṚ㩕ޫᡀ࠶˖ ޡ࠶᷀Ҷ MON 87427 ൠк䜘ᮤṚṧ૱Ⲵ 9 㩕ޫᡀ࠶˄㳻ⲭ䍘ǃ≤࠶ǃ 㜲㛚ǃ⚠࠶ǃ⻣≤ॆਸ⢙ǃADFǃNDFǃ䫉઼⼧˅DŽṩᦞᡰᴹ䈅傼ൠ⛩Ⲵᒣ൷ 㓿㥹⭈㟖༴⨶Ⲵ MON 87427 оᑨ㿴ሩ➗ѻ䰤Ⲵ㩕ޫᡀ࠶⋑ᴹ㔏䇑ᆖˈ٬ ᱮ㪇ᐞᔲ˄p>0.05˅˄㺘 26˅DŽնྲ᷌࠶ᔰ䈅傼ൠ⛩䘋㹼࠶᷀ˈMON 87427 ઼ሩ➗൘ 1 њ䈅傼ൠ⛩˄⡡㦧ॾᐎ Jefferson 䜑˅Ⲵњ࡛㩕ޫᡀ࠶˄⻣≤ॆਸ⢙ǃ ࠶઼㳻ⲭ䍘˅кᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲ˄㺘 27˅DŽণ֯㺘⧠ᱮ㪇ᐞᔲˈMON 87427≥ 䘉 3 ᡀ࠶Ⲵᒣ൷٬൷༴Ҿ୶ъॆ৲➗૱ᒣ൷٬Ⲵ 99%ᇩ䇨४䰤ˈҏ൘ ILSI ⢙㩕ޫᮠᦞᓃ઼᮷⥞ᣕ䚃Ⲵᮠᦞ㤳ത˄㺘 28˅DŽഐ↔ˈӾ伏૱઼侢ᯉ ᆹޘᡆ㩕ޫ䀂ᓖᶕⴻˈ䘉Ӌᐞᔲ⋑ᴹ⭏⢙ᆖѹDŽ 102 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘23. MON 87427ԕ৺ᑨ㿴ሩ➗Ⲵᣇ㩕ޫᡀ࠶Ⲵ≷ᙫᒣ൷٬ ૱ᦞ୶ъॆ ˅ሩ➗ᒣ൷٬ – ᐞᔲ˄MON 87427 ᒣ൷٬ MON 87427 ᑨ㿴ሩ➗ ᒣ൷٬ᗇࠪ ˅.S.E˄S.E.˅ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅ ᒣ൷٬ *S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ Ⲵᇩ䇨४䰤˄ᒣ൷٬ ˄㤳ത˅ ˄㤳ത˅ ˅㤳ത˄ ˅٬-㤳ത˅ л䲀ˈк䲀 ˄p˄ ᣇ㩕ޫᡀ࠶˄ᒢ䟽%˅ Ἵ䞨 0.96 (0.031) 1.02 (0.031) -0.060 (0.022) -0.10, -0.016 0.008 0.73, 1.23 (0.87 - 1.04) (0.94 - 1.12) (-0.12 - 0.032) (0.82 - 1.07) ỹᆀ㌆ 0.14 (0.028) 0.15 (0.029) -0.0054 (0.0082) -0.024, 0.013 0.524 0.024, 0.29 (0.098 - 0.21) (0.11 - 0.21) (-0.028 - 0.025) (0.092 - 0.21) ୶ъॆ৲➗૱ 95%㖞ؑ䲀лⲴ 99%ᇩ䇨४䰤ˈྲ᷌Ѫ䍏ᮠࡉ䇮Ѫ 0DŽ * 103 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᙫ≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬ ୶ъॆ৲➗૱ ˅ᑨ㿴ሩ➗ᒣ൷٬ – MON 87427² ᑨ㿴ሩ➗ 4 ᐞᔲ˄MON 87427 ᒣ൷٬ S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ ᇩ䇨४䰤 5˄S.E.˅ ᒣ൷٬˄S.E.˅³ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷٬ ˅㤳ത˄ ˅٬-㤳ത˅ ˄㤳ത˅ ˄㤳ത˅ л䲀, к䲀 ˄p˄ ᑨ㿴ᡀ࠶˄ᒢส%˅ ⚠࠶ 1.58 (0.036) 1.56 (0.038) 0.013 (0.042) -0.074, 0.099 0.765 1.13, 1.97 (1.43 - 1.81) (1.48 - 1.67) (-0.14 - 0.14) (1.18 - 1.82) ⻣≤ॆਸ⢙ 84.88 (0.56) 84.51 (0.57) 0.37 (0.33) -0.40, 1.14 0.305 80.77, 89.46 (83.60 - 86.33) (82.96 - 85.76) (-0.87 - 1.63) (82.26 - 87.17) ≤࠶˄⒯ส%˅ 11.62 (0.46) 11.41 (0.46) 0.22 (0.21) -0.27, 0.71 0.337 7.56, 14.80 (10.90 - 13.30) (10.20 - 12.40) (-0.30 - 1.10) (9.31 - 12.70) 㳻ⲭ䍘 10.05 (0.63) 10.26 (0.63) -0.21 (0.38) -1.08, 0.66 0.594 5.79, 13.43 (8.46 - 11.35) (8.62 - 11.92) (-1.50 - 1.20) (8.07 - 12.13) ᙫ㜲㛚䞨 3.50 (0.13) 3.69 (0.13) -0.19 (0.075) -0.36, -0.015 0.036 2.12, 5.35 (3.13 - 3.83) (3.47 - 3.98) (-0.52 - 0.11) (2.90 - 4.30) 㓔㔤˄ᒢส%˅ 䞨ᙗ⍇⏔㓔㔤˄ADF˅ 3.37 (0.23) 3.19 (0.23) 0.18 (0.27) -0.43, 0.79 0.521 1.84, 4.39 (2.67 - 4.27) (2.80 - 3.54) (-0.27 - 1.09) (2.29 - 4.27) 104 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬ ୶ъॆ৲➗૱ ˅ᑨ㿴ሩ➗ᒣ൷٬ – MON 87427² ᑨ㿴ሩ➗ 4 ᐞᔲ˄MON 87427 ᒣ൷٬ S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ ᇩ䇨४䰤 5˄S.E.˅ ᒣ൷٬˄S.E.˅³ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷٬ ˅㤳ത˄ ˅٬-㤳ത˅ ˄㤳ത˅ ˄㤳ത˅ л䲀, к䲀 ˄p˄ 㓔㔤˄ᒢส%˅ ѝᙗ⍇⏔㓔㔤˄NDF˅ 10.00 (0.51) 10.12 (0.51) -0.12 (0.24) -0.68, 0.43 0.628 5.69, 11.81 (9.17 - 10.97) (9.21 - 11.27) (-0.90 - 0.98) (7.06 - 10.66) ᙫ㟣伏㓔㔤˄TDF˅ 13.00 (0.37) 13.05 (0.37) -0.044 (0.24) -0.53, 0.44 0.854 8.67, 15.32 (12.13 - 14.35) (12.64 - 13.75) (-0.67 - 1.07) (10.25 - 14.30) ≘ส䞨˄ᒢส%˅ щ≘䞨 0.75 (0.061) 0.76 (0.061) -0.0061 (0.033) -0.082, 0.069 0.857 0.32, 1.12 (0.61 - 0.89) (0.55 - 0.90) (-0.15 - 0.080) (0.58 - 0.98) ㋮≘䞨 0.48 (0.024) 0.49 (0.025) -0.010 (0.015) -0.040, 0.020 0.501 0.24, 0.68 (0.40 - 0.55) (0.39 - 0.56) (-0.079 - 0.065) (0.34 - 0.57) ཙߜ≘䞨 0.64 (0.041) 0.64 (0.042) -0.0025 (0.025) -0.059, 0.054 0.920 0.34, 0.92 (0.54 - 0.71) (0.48 - 0.73) (-0.099 - 0.064) (0.52 - 0.78) 㜡≘䞨 0.24 (0.010) 0.24 (0.010) -0.0022 (0.0068) -0.018, 0.013 0.750 0.14, 0.30 (0.21 - 0.27) (0.21 - 0.26) (-0.015 - 0.020) (0.18 - 0.26) 105 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬ ୶ъॆ৲➗૱ ˅ᑨ㿴ሩ➗ᒣ൷٬ – MON 87427² ᑨ㿴ሩ➗ 4 ᐞᔲ˄MON 87427 ᒣ൷٬ S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ ᇩ䇨४䰤 5˄S.E.˅ ᒣ൷٬˄S.E.˅³ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷٬ ˅㤳ത˄ ˅٬-㤳ത˅ ˄㤳ത˅ ˄㤳ത˅ л䲀, к䲀 ˄p˄ ≘ส䞨˄ᒢส%˅ 䉧≘䞨 1.87 (0.15) 1.89 (0.15) -0.020 (0.077) -0.20, 0.16 0.801 0.77, 2.84 (1.53 - 2.24) (1.38 - 2.28) (-0.35 - 0.20) (1.46 - 2.49) ⭈≘䞨 0.38 (0.018) 0.38 (0.018) 0.0012 (0.0098) -0.021, 0.024 0.906 0.23, 0.52 (0.34 - 0.43) (0.31 - 0.42) (-0.038 - 0.033) (0.32 - 0.43) 㓴≘䞨 0.30 (0.013) 0.30 (0.013) -0.0014 (0.0081) -0.018, 0.015 0.867 0.16, 0.39 (0.27 - 0.34) (0.23 - 0.34) (-0.045 - 0.033) (0.22 - 0.33) ᔲӞ≘䞨 0.35 (0.026) 0.36 (0.027) -0.0018 (0.014) -0.035, 0.032 0.901 0.16, 0.53 (0.29 - 0.42) (0.26 - 0.42) (-0.081 - 0.039) (0.27 - 0.46) Ӟ≘䞨 1.23 (0.11) 1.25 (0.11) -0.022 (0.060) -0.16, 0.12 0.725 0.43, 1.95 (0.97 - 1.52) (0.89 - 1.56) (-0.29 - 0.13) (0.93 - 1.69) 䎆≘䞨 0.30 (0.012) 0.30 (0.013) -0.0020 (0.0072) -0.018, 0.014 0.782 0.19, 0.40 (0.27 - 0.33) (0.25 - 0.33) (-0.024 - 0.026) (0.26 - 0.34) 106 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬ ୶ъॆ৲➗૱ ˅ᑨ㿴ሩ➗ᒣ൷٬ – MON 87427² ᑨ㿴ሩ➗ 4 ᐞᔲ˄MON 87427 ᒣ൷٬ S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ ᇩ䇨४䰤 5˄S.E.˅ ᒣ൷٬˄S.E.˅³ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷٬ ˅㤳ത˄ ˅٬-㤳ത˅ ˄㤳ത˅ ˄㤳ത˅ л䲀, к䲀 ˄p˄ ≘ส䞨˄ᒢส%˅ 㳻≘䞨 0.24 (0.019) 0.24 (0.019) 0.00043 (0.0094) -0.021, 0.022 0.964 0.11, 0.29 (0.20 - 0.29) (0.20 - 0.27) (-0.015 - 0.024) (0.17 - 0.25) 㤟щ≘䞨 0.51 (0.040) 0.52 (0.040) -0.0088 (0.023) -0.063, 0.045 0.714 0.23, 0.75 (0.40 - 0.60) (0.38 - 0.61) (-0.10 - 0.052) (0.39 - 0.66) 㝟≘䞨 0.90 (0.067) 0.90 (0.067) -0.0045 (0.032) -0.078, 0.069 0.889 0.40, 1.24 (0.74 - 1.08) (0.65 - 1.06) (-0.15 - 0.12) (0.66 - 1.07) э≘䞨 0.47 (0.033) 0.48 (0.033) -0.011 (0.022) -0.062, 0.040 0.625 0.24, 0.66 (0.38 - 0.52) (0.36 - 0.58) (-0.063 - 0.052) (0.38 - 0.59) 㣿≘䞨 0.35 (0.020) 0.35 (0.020) -0.0022 (0.013) -0.032, 0.028 0.871 0.20, 0.46 (0.29 - 0.39) (0.28 - 0.39) (-0.042 - 0.033) (0.28 - 0.41) 㢢≘䞨 0.054 (0.0032) 0.053 (0.0033) 0.00070 (0.0032) -0.0067, 0.0081 0.835 0.032, 0.069 (0.045 - 0.064) (0.042 - 0.065) (-0.015 - 0.013) (0.039 - 0.063) 107 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬ ୶ъॆ৲➗૱ ˅ᑨ㿴ሩ➗ᒣ൷٬ – MON 87427² ᑨ㿴ሩ➗ 4 ᐞᔲ˄MON 87427 ᒣ൷٬ S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ ᇩ䇨४䰤 5˄S.E.˅ ᒣ൷٬˄S.E.˅³ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷٬ ˅㤳ത˄ ˅٬-㤳ത˅ ˄㤳ത˅ ˄㤳ത˅ л䲀, к䲀 ˄p˄ ≘ส䞨˄ᒢส%˅ 䞚≘䞨 0.29 (0.029) 0.30 (0.029) -0.0041 (0.026) -0.057, 0.048 0.874 0.077, 0.45 (0.18 - 0.38) (0.21 - 0.39) (-0.12 - 0.11) (0.11 - 0.43) 㕜≘䞨 0.48 (0.029) 0.49 (0.029) -0.0015 (0.017) -0.040, 0.037 0.930 0.25, 0.67 (0.41 - 0.55) (0.37 - 0.56) (-0.089 - 0.049) (0.38 - 0.58) 㜲㛚䞨˄ঐᙫ㜲㛚䞨%˅ 16:0 ἅᾸ䞨 10.91 (0.26) 10.54 (0.26) 0.37 (0.065) 0.22, 0.52 <0.001 6.42, 15.23 (10.44 - 11.52) (10.15 - 11.08) (0.14 - 0.59) (9.13 - 12.33) 18:0 ⺜㜲䞨 1.97 (0.091) 1.90 (0.091) 0.070 (0.028) 0.0048, 0.13 0.038 0.87, 2.88 (1.81 - 2.17) (1.77 - 2.07) (-0.028 - 0.18) (1.54 - 2.38) 18:1 ⋩䞨 c 24.28 (0.92) 23.52 (0.92) 0.76 (0.23) 0.23, 1.28 0.010 11.30, 43.27 (22.84 - 26.62) (21.74 - 25.71) (0.13 - 1.20) (21.39 - 34.71) 18:2 ӊ⋩䞨 60.84 (1.28) 62.06 (1.28) -1.22 (0.29) -1.88, -0.55 0.002 41.35, 74.78 (57.61 - 62.70) (59.18 - 64.09) (-1.69 - -0.46) (49.38 - 63.16) 108 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬ ୶ъॆ৲➗૱ ˅ᑨ㿴ሩ➗ᒣ൷٬ – MON 87427² ᑨ㿴ሩ➗ 4 ᐞᔲ˄MON 87427 ᒣ൷٬ S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ ᇩ䇨४䰤 5˄S.E.˅ ᒣ൷٬˄S.E.˅³ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷٬ ˅㤳ത˄ ˅٬-㤳ത˅ ˄㤳ത˅ ˄㤳ത˅ л䲀, к䲀 ˄p˄ 㜲㛚䞨˄ঐᙫ㜲㛚䞨%˅ 18:3 ӊ哫⋩䞨 1.20 (0.014) 1.20 (0.014) -0.0012 (0.015) -0.035, 0.033 0.935 0.78, 1.52 (1.13 - 1.26) (1.18 - 1.22) (-0.088 - 0.043) (0.97 - 1.35) 20:0 㣡⭏䞨 0.42 (0.030) 0.41 (0.030) 0.016 (0.0043) 0.0063, 0.026 0.005 0.15, 0.67 (0.37 - 0.48) (0.37 - 0.46) (-0.0022 - 0.034) (0.32 - 0.53) 20:1 Ҽॱ⻣✟䞨 0.21 (0.0080) 0.21 (0.0080) -0.00097 (0.0017) -0.0049, 0.0029 0.583 0.12, 0.36 (0.19 - 0.23) (0.20 - 0.23) (-0.0049 - 0.0033) (0.21 - 0.31) 22:0 ኡ㩞䞨 0.17 (0.018) 0.16 (0.018) 0.0076 (0.0050) -0.0039, 0.019 0.167 0, 0.32 (0.14 - 0.23) (0.14 - 0.20) (-0.0099 - 0.031) (0.057 - 0.23) ⸯ⢙䍘 䫉˄ᒢส%˅ 0.0060 (0.00063) 0.0055 (0.00063) 0.00049 (0.00033) -0.00027, 0.0013 0.176 0.0019, 0.0076 (0.0048 - 0.0079) (0.0046 - 0.0076) (-0.00037 - 0.0017) (0.0038 - 0.0068) 䬌˄mg/kg dwt˅ 1.63 (0.11) 1.71 (0.12) -0.085 (0.11) -0.33, 0.16 0.458 0.17, 3.48 (1.21 - 2.07) (1.49 - 1.99) (-0.42 - 0.18) (1.10 - 2.62) 109 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬ ୶ъॆ৲➗૱ ˅ᑨ㿴ሩ➗ᒣ൷٬ – MON 87427² ᑨ㿴ሩ➗ 4 ᐞᔲ˄MON 87427 ᒣ൷٬ S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ ᇩ䇨४䰤 5˄S.E.˅ ᒣ൷٬˄S.E.˅³ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷٬ ˅㤳ത˄ ˅٬-㤳ത˅ ˄㤳ത˅ ˄㤳ത˅ л䲀, к䲀 ˄p˄ ⸯ⢙䍘 䫱˄mg/kg dwt˅ 23.61 (0.78) 23.03 (0.79) 0.58 (0.61) -0.82, 1.98 0.368 11.42, 28.01 (22.21 - 25.84) (20.66 - 25.57) (-2.12 - 2.11) (16.55 - 24.10) 䭱˄ᒢส%˅ 0.13 (0.0033) 0.13 (0.0033) -0.00021 (0.0034) -0.0080, 0.0076 0.952 0.080, 0.16 (0.13 - 0.14) (0.12 - 0.14) (-0.0062 - 0.010) (0.11 - 0.15) 䭠˄mg/kg dwt˅ 7.91 (1.06) 8.07 (1.06) -0.16 (0.27) -0.71, 0.39 0.567 0, 12.67 (5.52 - 9.40) (4.89 - 9.82) (-0.83 - 0.83) (4.00 - 9.17) ⼧˄ᒢส%˅ 0.34 (0.0034) 0.34 (0.0036) -0.0071 (0.0050) -0.018, 0.0040 0.185 0.24, 0.42 (0.32 - 0.35) (0.33 - 0.35) (-0.020 - 0.0053) (0.28 - 0.37) 䫮˄ᒢส%˅ 0.40 (0.0074) 0.40 (0.0077) -0.0045 (0.0073) -0.019, 0.010 0.546 0.24, 0.54 (0.38 - 0.42) (0.38 - 0.43) (-0.029 - 0.021) (0.33 - 0.46) 䬼˄mg/kg dwt˅ 22.67 (1.06) 23.99 (1.07) -1.32 (1.00) -3.62, 0.99 0.225 11.46, 30.37 (20.99 - 25.42) (21.65 - 28.08) (-5.63 - 3.29) (17.30 - 25.45) 110 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬ ୶ъॆ৲➗૱ ˅ᑨ㿴ሩ➗ᒣ൷٬ – MON 87427² ᑨ㿴ሩ➗ 4 ᐞᔲ˄MON 87427 ᒣ൷٬ S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ ᇩ䇨४䰤 5˄S.E.˅ ᒣ൷٬˄S.E.˅³ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷٬ ˅㤳ത˄ ˅٬-㤳ത˅ ˄㤳ത˅ ˄㤳ത˅ л䲀, к䲀 ˄p˄ 㔤⭏㍐˄mg/kg dwt˅ ਦ䞨 0.36 (0.025) 0.39 (0.025) -0.030 (0.030) -0.099, 0.040 0.347 0.11, 0.61 (0.28 - 0.43) (0.29 - 0.49) (-0.097 - 0.078) (0.24 - 0.57) ✏䞨 27.22 (2.15) 27.71 (2.18) -0.48 (1.34) -3.22, 2.26 0.722 7.89, 49.83 (22.56 - 33.37) (22.61 - 33.26) (-3.30 - 2.66) (20.63 - 43.08) 㔤⭏㍐ A 1.01 (0.050) 0.96 (0.051) 0.057 (0.043) -0.029, 0.14 0.186 0.38, 1.68 (0.88 - 1.21) (0.76 - 1.16) (-0.094 - 0.21) (0.58 - 1.50) 㔤⭏㍐ B1 2.97 (0.19) 2.88 (0.20) 0.084 (0.16) -0.28, 0.45 0.606 2.21, 3.65 (2.58 - 3.41) (2.48 - 3.41) (-0.44 - 0.45) (2.41 - 3.48) 㔤⭏㍐ B2 2.09 (0.37) 1.93 (0.37) 0.16 (0.33) -0.59, 0.92 0.630 0, 4.47 (1.17 - 3.56) (1.32 - 2.58) (-0.72 - 1.23) (1.28 - 3.29) 㔤⭏㍐ B6 7.48 (0.60) 7.71 (0.60) -0.23 (0.41) -1.16, 0.70 0.589 2.57, 12.07 (5.91 - 8.69) (5.67 - 9.61) (-1.40 - 1.76) (5.24 - 10.29) 111 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬ ୶ъॆ৲➗૱ ˅ᑨ㿴ሩ➗ᒣ൷٬ – MON 87427² ᑨ㿴ሩ➗ 4 ᐞᔲ˄MON 87427 ᒣ൷٬ S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ ᇩ䇨४䰤 5˄S.E.˅ ᒣ൷٬˄S.E.˅³ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷٬ ˅㤳ത˄ ˅٬-㤳ത˅ ˄㤳ത˅ ˄㤳ത˅ л䲀, к䲀 ˄p˄ 㔤⭏㍐˄mg/kg dwt˅ 㔤⭏㍐ E 13.14 (2.09) 13.46 (2.10) -0.31 (0.86) -2.05, 1.43 0.718 0, 25.61 (7.04 - 17.44) (10.13 - 18.10) (-6.54 - 4.52) (6.67 - 17.34) ¹dwt=ᒢ䟽DŽ ² MON 87427 ௧㥹⭈㟖༴⨶DŽ S.E.) = ḷ߶ᐞDŽ)ˈᒣ൷٬Ѫᴰሿᒣᯩᒣ൷٬ ³ 4 ᑨ㿴ሩ➗о MON 87427 Ѫ䘁ㅹสഐޣ㌫ˈ௧㥹⭈㟖༴⨶DŽ ୶ъॆ૱Ⲵᒣ൷٬൘ 95%㖞ؑ䲀лⲴ 99%ᇩ䇨४䰤ˈྲ᷌ᱟ䍏ᮠቡ䇮ᇊѪ 0DŽ 5 112 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᙫ≶㺘 25. MON 87427 ઼ᑨ㿴ሩ➗㊭㋂⅑⭏ԓ䉒ӗ⢙Ⲵᡰᴹ䈅傼⛩ᒣ൷٬ ୶ъॆ৲➗૱ ˅ᑨ㿴ሩ➗ᒣ൷٬ – MON 87427² ᑨ㿴ሩ➗ 4 ᐞᔲ˄MON 87427 ᒣ൷٬ S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ ᇩ䇨४䰤 5˄S.E.˅ ᒣ൷٬˄S.E.˅³ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷٬ ˅㤳ത˄ ˅٬-㤳ത˅ ˄㤳ത˅ ˄㤳ത˅ л䲀, к䲀 ˄p˄ ⅑⭏ԓ䉒ӗ⢙˄μg/g dwt˅ 䱯兿䞨 2348.63 (58.17) 2387.92 (60.24) -39.29 (81.45) -221.69, 143.10 0.640 1070.41, 2955.86 (2188.55 - 2559.19) (2236.10 - 2500.00) (-171.29 - 209.93) (1588.35 - 2630.98) ሩ-俉䉶䞨 204.94 (17.45) 205.00 (17.54) -0.060 (8.82) -20.17, 20.05 0.994 58.74, 313.97 (166.11 - 260.43) (162.58 - 252.26) (-28.53 - 32.92) (124.16 - 250.30) ¹dwt=ᒢ䟽DŽ ² MON 87427 ௧㥹⭈㟖༴⨶DŽ S.E.) = ḷ߶ᐞDŽ)ˈᒣ൷٬Ѫᴰሿᒣᯩᒣ൷٬ ³ 4 ᑨ㿴ሩ➗о MON 87427 Ѫ䘁ㅹสഐޣ㌫ˈ௧㥹⭈㟖༴⨶DŽ ୶ъॆ૱Ⲵᒣ൷٬൘ 95%㖞ؑ䲀лⲴ 99%ᇩ䇨४䰤ˈྲ᷌ᱟ䍏ᮠቡ䇮ᇊѪ 0DŽ 5 113 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᙫ≶㺘26. MON 87427઼ᑨ㿴ሩ➗ൠк䜘ᮤṚ㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬ ୶ъॆ৲➗૱ ˅ᑨ㿴ሩ➗ᒣ൷٬ – MON 87427² ᑨ㿴ሩ➗ 4 ᐞᔲ˄MON 87427 ᒣ൷٬ S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ ᇩ䇨४䰤 5˄S.E.˅ ᒣ൷٬˄S.E.˅³ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷٬ ˅㤳ത˄ ˅٬-㤳ത˅ ˄㤳ത˅ ˄㤳ത˅ л䲀, к䲀 ˄p˄ ᑨ㿴ᡀ࠶˄ᒢส%˅ ⚠࠶ 4.73 (0.23) 4.86 (0.23) -0.13 (0.19) -0.53, 0.27 0.508 2.66, 6.48 (4.39 - 5.13) (3.99 - 5.84) (-0.74 - 0.66) (3.70 - 5.95) ⻣≤ॆਸ⢙ 87.23 (0.90) 86.69 (0.91) 0.54 (0.49) -0.46, 1.54 0.277 80.13, 94.05 (86.21 - 89.23) (83.80 - 88.92) (-1.59 - 2.61) (83.23 - 90.37) ≤࠶˄⒯ส%˅ 68.71 (2.30) 69.76 (2.32) -1.05 (1.06) -3.50, 1.40 0.350 51.70, 86.22 (62.70 - 73.10) (64.10 - 75.00) (-5.90 - 5.70) (61.00 - 76.00) 㳻ⲭ䍘 6.44 (0.75) 6.78 (0.76) -0.34 (0.39) -1.25, 0.57 0.413 1.34, 11.57 (4.48 - 7.40) (5.17 - 8.94) (-2.00 - 1.26) (4.37 - 9.31) ᙫ㜲㛚 1.60 (0.17) 1.69 (0.18) -0.092 (0.25) -0.65, 0.46 0.720 0.44, 3.33 (1.09 - 1.85) (0.58 - 2.28) (-1.11 - 1.18) (0.78 - 3.16) 㓔㔤˄ᒢส%˅ ADF 24.96 (0.97) 26.74 (1.03) -1.78 (1.42) -4.65, 1.09 0.216 14.84, 38.51 (21.08 - 29.00) (20.27 - 32.16) (-8.15 - 3.58) (21.33 - 35.92) 114 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᙫ≶㺘 26. MON 87427 ઼ᑨ㿴ሩ➗ൠк䜘ᮤṚ㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬ ୶ъॆ৲➗૱ ˅ᑨ㿴ሩ➗ᒣ൷٬ – MON 87427² ᑨ㿴ሩ➗ 4 ᐞᔲ˄MON 87427 ᒣ൷٬ S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ ᇩ䇨४䰤 5˄S.E.˅ ᒣ൷٬˄S.E.˅³ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷٬ ˅㤳ത˄ ˅٬-㤳ത˅ ˄㤳ത˅ ˄㤳ത˅ л䲀, к䲀 ˄p˄ 㓔㔤˄ᒢส%˅ 39.79 (1.32) 38.12 (1.38) 1.67 (1.76) -2.32, 5.65 0.368 25.12, 54.99 NDF (36.14 - 43.70) (33.07 - 43.43) (-1.55 - 4.79) (29.68 - 60.16) ⸯ⢙䍘˄ᒢส%˅ 0.19 (0.010) 0.19 (0.011) -0.0083 (0.011) -0.031, 0.014 0.455 0.075, 0.29 䫉 (0.14 - 0.22) (0.15 - 0.25) (-0.063 - 0.036) (0.10 - 0.24) ⼧ 0.24 (0.021) 0.24 (0.021) -0.0050 (0.013) -0.032, 0.022 0.708 0.063, 0.37 (0.20 - 0.31) (0.19 - 0.31) (-0.074 - 0.038) (0.16 - 0.31) ¹dwt=ᒢ䟽DŽ ² MON 87427 ௧㥹⭈㟖༴⨶DŽ S.E.) = ḷ߶ᐞDŽ)ˈᒣ൷٬Ѫᴰሿᒣᯩᒣ൷٬ ³ 4 ᑨ㿴ሩ➗о MON 87427 Ѫ䘁ㅹสഐޣ㌫ˈ௧㥹⭈㟖༴⨶DŽ ୶ъॆ૱Ⲵᒣ൷٬൘ 95%㖞ؑ䲀лⲴ 99%ᇩ䇨४䰤ˈྲ᷌ᱟ䍏ᮠቡ䇮ᇊѪ 0DŽ 5 115 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘27. MON 87427઼ᑨ㿴ሩ➗ᒣ൷٬㺘⧠ࠪ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶≷ᙫ ୶ъॆ৲➗૱ ˅ሩ➗٬ – ٬ ᒣ൷ᐞ˄MON 87427 MON 87427² ᑨ㿴ሩ➗ 4 MON 87427 ࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷ᐞ 95%㖞ؑ४䰤 ᇩ䇨४䰤 5 ˅㤳ത˄ 3 ᒣ൷٬ ᒣ൷٬ ˄ᖃҾሩ➗%˅ л䲀, к䲀 ˄㤳ത˅ ᡰᴹ䈅傼⛩ᒣ൷٬ᆈ൘ᱮ㪇ᙗᐞᔲ ᑨ㿴ᡀ࠶˄ᒢส%˅ ᙫ㜲㛚 3.50 3.69 -5.09 0.036 3.13 - 3.83 2.12, 5.35 ㊭㋂㜲㛚䞨˄ঐᙫ㜲㛚䞨%˅ 16:0 ἅᾸ䞨 10.91 10.54 3.52 <0.001 10.44 - 11.52 6.42, 15.23 18:0 ⺜㜲䞨 1.97 1.90 3.67 0.038 1.81 - 2.17 0.87, 2.88 18:1 ⋩䞨 24.28 23.52 3.22 0.010 22.84 - 26.62 11.30, 43.27 18:2 ӊ⋩䞨 60.84 62.06 -1.96 0.002 57.61 - 62.70 41.35, 74.78 20:0 㣡⭏䞨 0.42 0.41 4.00 0.005 0.37 - 0.48 0.15, 0.67 ㊭㋂ᣇ㩕ޫᡀ࠶˄ᒢส%˅ Ἵ䞨 0.96 1.02 -5.92 0.008 0.87 - 1.04 0.73, 1.23 116 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅㺘 27. MON 87427 ઼ᑨ㿴ሩ➗ᒣ൷٬㺘⧠ࠪ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶≷ᙫ˄㔝 ୶ъॆ৲➗૱ ˅ሩ➗٬ – ٬ ᒣ൷ᐞ˄MON 87427 MON 87427² ᑨ㿴ሩ➗ 4 MON 87427 ࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷ᐞ 95%㖞ؑ४䰤 ᇩ䇨४䰤 5 ˅㤳ത˄ 3 ᒣ൷٬ ᒣ൷٬ ˄ᖃҾሩ➗%˅ л䲀, к䲀 ˄㤳ത˅ ањԕк䈅傼⛩ᮠ٬ᆈ൘ᱮ㪇ᙗᐞᔲ ㊭㋂㜲㛚䞨˄ঐᙫ㜲㛚䞨%˅ 16:0 ἅᾸ䞨 - ൠ⛩˖ARNE 11.49 10.99 4.53 <0.001 11.47 - 11.52 6.42, 15.23 - ൠ⛩˖IARL 10.72 10.44 2.66 0.007 10.58 - 10.85 6.42, 15.23 - ൠ⛩˖ILWY 10.54 10.21 3.25 <0.001 10.44 - 10.65 6.42, 15.23 18:1 ⋩䞨 - ൠ⛩˖ARNE 26.34 25.35 3.93 <0.001 26.16 - 26.62 11.30, 43.27 - ൠ⛩˖IARL 22.91 21.95 4.41 0.002 22.84 - 22.98 11.30, 43.27 - ൠ⛩˖ILWY 23.58 23.24 1.44 0.043 23.29 - 23.78 11.30, 43.27 18:2 ӊ⋩䞨 - ൠ⛩˖ARNE 57.94 59.56 -2.72 <0.001 57.61 - 58.13 41.35, 74.78 - ൠ⛩˖IARL 62.57 63.90 -2.09 <0.001 62.49 - 62.70 41.35, 74.78 - ൠ⛩˖ILWY 62.01 62.72 -1.13 0.005 61.68 - 62.32 41.35, 74.78 117 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅㺘 27. MON 87427 ઼ᑨ㿴ሩ➗ᒣ൷٬㺘⧠ࠪ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶≷ᙫ˄㔝 ୶ъॆ৲➗૱ ˅ሩ➗٬ – ٬ ᒣ൷ᐞ˄MON 87427 MON 87427² ᑨ㿴ሩ➗ 4 MON 87427 ࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷ᐞ 95%㖞ؑ४䰤 ᇩ䇨४䰤 5 ˅㤳ത˄ 3 ᒣ൷٬ ᒣ൷٬ ˄ᖃҾሩ➗%˅ л䲀, к䲀 ˄㤳ത˅ ањԕк䈅傼⛩ᮠ٬ᆈ൘ᱮ㪇ᙗᐞᔲ ㊭㋂≘ส䞨˄ᒢส%˅ 㳻≘䞨 - ൠ⛩˖ARNE 0.29 0.27 6.48 0.043 0.28 - 0.29 0.11, 0.29 - ൠ⛩˖IARL 0.23 0.25 -7.29 0.018 0.22 - 0.23 0.11, 0.29 ㊭㋂㜲㛚䞨˄ঐᙫ㜲㛚䞨%˅ 18:3 ӊ哫⋩䞨 - ൠ⛩˖ARNE 1.15 1.19 -3.92 0.033 1.13 - 1.17 0.78, 1.52 - ൠ⛩˖IARL 1.24 1.20 3.35 0.014 1.22 - 1.26 0.78, 1.52 ㊭㋂㔤⭏㍐˄mg/kg dwt˅ 㔤⭏㍐ B2 - ൠ⛩˖ARNE 3.27 2.36 38.30 0.004 3.05 - 3.56 0, 4.47 - ൠ⛩˖IARL 1.41 1.93 -26.71 0.042 1.17 - 1.60 0, 4.47 118 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅㺘 27. MON 87427 ઼ᑨ㿴ሩ➗ᒣ൷٬㺘⧠ࠪ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶≷ᙫ˄㔝 ୶ъॆ৲➗૱ ˅ሩ➗٬ – ٬ ᒣ൷ᐞ˄MON 87427 MON 87427² ᑨ㿴ሩ➗ 4 MON 87427 ࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷ᐞ 95%㖞ؑ४䰤 ᇩ䇨४䰤 5 ˅㤳ത˄ 3 ᒣ൷٬ ᒣ൷٬ ˄ᖃҾሩ➗%˅ л䲀, к䲀 ˄㤳ത˅ অњ䈅傼⛩ᮠ٬ᆈ൘ᱮ㪇ᙗᐞᔲ ㊭㋂ᑨ㿴ᡀ࠶˄ᒢส%˅ ⻣≤ॆਸ⢙˄ൠ⛩˖IARL˅ 84.24 83.11 1.36 0.047 83.60 - 84.96 80.77, 89.46 ≤࠶˄⒯ส%˅˄ൠ⛩˖IARL˅ 10.93 10.40 5.13 0.043 10.90 - 11.00 7.56, 14.80 㳻ⲭ䍘˄ൠ⛩˖IARL˅ 10.60 11.73 -9.64 0.019 9.91 - 11.35 5.79, 13.43 ㊭㋂㓔㔤˄ᒢส%˅ ADF˄ൠ⛩˖ILWY˅ 3.78 3.05 23.75 0.020 3.33 - 4.27 1.84, 4.39 ㊭㋂≘ส䞨˄ᒢส%˅ ㋮≘䞨˄ൠ⛩˖IARL˅ 0.48 0.53 -9.19 0.033 0.45 - 0.49 0.24, 0.68 㜡≘䞨˄ൠ⛩˖IARL˅ 0.24 0.26 -5.95 0.012 0.24 - 0.25 0.14, 0.30 э≘䞨˄ൠ⛩˖IARL˅ 0.49 0.56 -11.21 0.037 0.46 - 0.51 0.24, 0.66 119 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅㺘 27. MON 87427 ઼ᑨ㿴ሩ➗ᒣ൷٬㺘⧠ࠪ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶≷ᙫ˄㔝 ୶ъॆ৲➗૱ ˅ሩ➗٬ – ٬ ᒣ൷ᐞ˄MON 87427 MON 87427² ᑨ㿴ሩ➗ 4 MON 87427 ࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷ᐞ 95%㖞ؑ४䰤 ᇩ䇨४䰤 5 ˅㤳ത˄ 3 ᒣ൷٬ ᒣ൷٬ ˄ᖃҾሩ➗%˅ л䲀, к䲀 ˄㤳ത˅ অњ䈅傼⛩ᮠ٬ᆈ൘ᱮ㪇ᙗᐞᔲ ㊭㋂≘ส䞨˄ᒢส%˅ 0.062 0.052 19.32 0.006 0.059 - 0.064 0.032, 0.069 㢢≘䞨˄ൠ⛩˖ARNE˅ ㊭㋂㜲㛚䞨˄ঐᙫ㜲㛚䞨%˅ 18:0 ⺜㜲䞨˄ൠ⛩˖ARNE˅ 2.17 2.04 6.43 0.002 2.16 - 2.17 0.87, 2.88 20:0 㣡⭏䞨˄ൠ⛩˖ARNE˅ 0.48 0.46 4.63 0.002 0.47 - 0.48 0.15, 0.67 22:0 ኡ㩞䞨˄ൠ⛩˖ARNE˅ 0.21 0.19 11.00 0.007 0.21 - 0.23 0, 0.32 ㊭㋂ⸯ⢙䍘 䫉˄ᒢส%˅˄ൠ⛩˖ARNE˅ 0.0077 0.0067 14.03 0.024 0.0075 - 0.0079 0.0019, 0.0076 䬼˄mg/kg dw˅˄ൠ⛩˖IARL˅ 23.54 26.51 -11.20 0.010 22.45 - 24.61 11.46, 30.37 ㊭㋂㔤⭏㍐˄mg/kg dwt˅ ਦ䞨˄ൠ⛩˖IARL˅ 0.36 0.45 -19.59 0.020 0.31 - 0.40 0.11, 0.61 120 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅㺘 27. MON 87427 ઼ᑨ㿴ሩ➗ᒣ൷٬㺘⧠ࠪ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶≷ᙫ˄㔝 ୶ъॆ৲➗૱ ˅ሩ➗٬ – ٬ ᒣ൷ᐞ˄MON 87427 MON 87427² ᑨ㿴ሩ➗ 4 MON 87427 ࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷ᐞ 95%㖞ؑ४䰤 ᇩ䇨४䰤 5 ˅㤳ത˄ 3 ᒣ൷٬ ᒣ൷٬ ˄ᖃҾሩ➗%˅ л䲀, к䲀 ˄㤳ത˅ অњ䈅傼⛩ᮠ٬ᆈ൘ᱮ㪇ᙗᐞᔲ ㊭㋂ᣇ㩕ޫᡀ࠶˄ᒢส%˅ ỹᆀ㌆˄ൠ⛩˖ARNE˅ 0.11 0.13 -18.51 0.031 0.11 - 0.11 0.024, 0.29 ൠк䜘ᮤṚⲴᑨ㿴ᡀ࠶˄ᒢส%˅ ⻣≤ॆਸ⢙˄ൠ⛩˖IARL˅ 86.46 84.12 2.78 0.029 86.21 - 86.75 80.13, 94.05 ≤࠶˄⒯ส%˅˄ൠ⛩˖IARL˅ 69.90 74.71 -6.44 0.008 67.70 - 71.20 51.70, 86.22 㳻ⲭ䍘˄ൠ⛩˖IARL˅ 7.03 8.63 -18.59 0.037 6.75 - 7.40 1.34, 11.57 ¹dwt=ᒢ䟽˗ൠ⛩˖ARNE = 䱯㛟㢢ᐎⲴ JacksonˈIARL = ⡡㦧ॾᐎⲴ JeffersonˈILWY = Ժ࡙䈪ԺᐎⲴ StarkDŽ ² MON 87427 ௧㥹⭈㟖༴⨶DŽ ᒣ൷٬Ѫᴰሿᒣᯩᒣ൷٬DŽ ³ 4 ᑨ㿴ሩ➗о MON 87427 Ѫ䘁ㅹสഐޣ㌫ˈ௧㥹⭈㟖༴⨶DŽ ୶ъॆ૱Ⲵᒣ൷٬൘ 95%㖞ؑ䲀лⲴ 99%ᇩ䇨४䰤ˈྲ᷌ᱟ䍏ᮠቡ䇮ᇊѪ 0DŽ 5 121 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘28. ਁ㺘᮷⥞઼ILSIᮠᦞᓃѝ⦹㊣ൠк䜘ἽṚ઼㊭㋂ᡀ࠶Ⲵᮠ٬㤳ത ㊭㋂㓴㓷ᡀ࠶ 1 ਁ㺘᮷⥞ѝᮠ٬㤳ത 2 ILSI ᮠᦞᓃѝᮠ٬㤳ത 3 ㊭㋂㩕ޫᡀ࠶ਜ਼䟿 ᑨ㿴ᡀ࠶˄ᒢส%˅ ⚠࠶ 1.17 – 2.01a; 1.14 – 1.63b 0.616 – 6.282 ᣈਸ⻣≤ॆਸ⢙ 81.31 – 87.06a; 82.10 – 86.65b 77.4 – 89.5 ᙫ㜲㛚 2.95 – 4.40a; 3.16 – 4.23b 1.742 – 5.823 ≤࠶˄⒯ส%˅ 8.74 – 11.30a; 11.00 – 13.20b 6.1 – 40.5 㳻ⲭ䍘 8.27 – 13.33a; 8.55 – 12.19b 6.15 – 17.26 㓔㔤˄ᒢส%˅ 䞨ᙗ⍇⏔㓔㔤˄ADF˅ 1.82 – 4.48a; 1.14 – 4.41b 1.82 – 11.34 ѝᙗ⍇⏔㓔㔤˄NDF˅ 6.51 –12.28a; 6.08 – 10.36b 5.59 – 22.64 ᙫ㟣伏㓔㔤˄TDF˅ 10.65 – 16.26a; 10.24 – 14.56b 8.82 – 35.31 ≘ส䞨˄ᒢส%˅ щ≘䞨 0.60 – 1.04a; 0.63 – 0.96b 0.439 – 1.393 ㋮≘䞨 0.34 – 0.52a; 0.32 – 0.50b 0.119 –0.639 ཙߜ≘䞨 0.52 – 0.78a; 0.56 – 0.77b 0.335 – 1.208 㜡≘䞨 0.19 – 0.26a; 0.20 – 0.26b 0.125 – 0.514 䉧≘䞨 1.54 – 2.67a; 1.62 – 2.44b 0.965 – 3.536 ⭈≘䞨 0.33 – 0.43a; 0.31 – 0.42b 0.184 – 0.539 㓴≘䞨 0.25 – 0.37a; 0.24 – 0.34b 0.137 – 0.434 ᔲӞ≘䞨 0.30 – 0.48a; 0.30 – 0.44b 0.179 – 0.692 Ӟ≘䞨 1.02 – 1.87a; 1.06 – 1.65b 0.642 – 2.492 䎆≘䞨 0.26 – 0.33a; 0.25 – 0.31b 0.172 – 0.668 㳻≘䞨 0.17 – 0.26a; 0.16 – 0.30b 0.124 – 0.468 㤟щ≘䞨 0.43 – 0.72a; 0.43 – 0.63b 0.244 – 0.930 㝟≘䞨 0.74 – 1.21a;0.72–1.11b 0.462 – 1.632 э≘䞨 0.39 – 0.67a; 0.40 – 0.60b 0.235 – 0.769 㣿≘䞨 0.29 – 0.45a; 0.29 – 0.39b 0.224 – 0.666 㢢≘䞨 0.047 – 0.085a; 0.040 – 0.070b 0.0271 – 0.215 䞚≘䞨 0.13 – 0.43a; 0.12 – 0.41b 0.103 – 0.642 㕜≘䞨 0.42 – 0.62a; 0.41 – 0.58b 0.266 – 0.855 122 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ㊭㋂㓴㓷ᡀ࠶ 1 ਁ㺘᮷⥞ѝᮠ٬㤳ത 2 ILSI ᮠᦞᓃѝᮠ٬㤳ത 3 㜲㛚䞨˄ঐᙫ㜲㛚䞨%˅ 16:0 ἅᾸ䞨 8.80 – 13.33a; 9.53 – 12.33b 7.94 – 20.71 18:0 ⺜㜲䞨 1.36 – 2.14 a; 1.28 – 2.13b 1.02 – 3.40 18:1 ⋩䞨 19.50 – 33.71a; 19.59 – 31.09b 17.4 – 40.2 18:2 ӊ⋩䞨 49.31 – 64.70a; 55.17 – 65.65b 36.2 – 66.5 18:3 ӊ哫⋩䞨 0.89 – 1.56a; 1.00 – 1.38b 0.57 – 2.25 20:0 㣡⭏䞨 0.30 – 0.49a; 0.29 – 0.42b 0.279 – 0.965 20:1 Ҽॱ⻣✟䞨 0.17 – 0.29a; 0.17 – 0.31b 0.170 – 1.917 22:0 ኡ㩞䞨 0.069 – 0.28a; 0.059 – 0.33b 0.110 – 0.349 ⸯ⢙䍘 䫉˄ᒢส%˅ 0.0036 – 0.0068a; 0.0032 – 0.0070b 0.00127 – 0.02084 䬌˄mg/kg dwt˅ 1.14 – 3.43a; 1.29 – 4.16b 0.73 – 18.50 䫱˄mg/kg dwt˅ 14.17 – 23.40a; 14.37 – 24.66b 10.42 – 49.07 䭱˄ᒢส%˅ 0.091 – 0.14a; 0.095 – 0.14b 0.0594 – 0.194 䭠˄mg/kg dwt˅ 4.83 – 8.34a; 4.55 – 9.35b 1.69 – 14.30 ⼧˄ᒢส%˅ 0.24 – 0.37a; 0.26 – 0.38b 0.147 – 0.533 䫮˄ᒢส%˅ 0.29 – 0.39a; 0.32 – 0.45b 0.181 – 0.603 䬼˄mg/kg dwt˅ 16.78 – 28.17a; 18.12 – 30.44b 6.5 – 37.2 㔤⭏㍐˄mg/kg dw˅ ਦ䞨 0.19 – 0.35a; 0.22 – 0.42b 0.147 – 1.464 㔤⭏㍐ A[ –㜑㩍ঌ㍐] Not Available 0.19 – 46.81 a b 㔤⭏㍐ B1 [⺛㜪] 2.33 – 4.17 ; 2.71 – 4.78 1.26 – 40.00 a b 㔤⭏㍐ B2 [Ṩ哴㍐] 0.94 – 2.42 ; 1.46 – 2.81 0.50 – 2.36 a b 㔤⭏㍐ B3 [✏䞨] 15.07 – 32.38 ; 13.64 – 42.60 10.37 – 46.94 a b 㔤⭏㍐ B6 [ੑ㍐] 4.93 – 7.53 ; 4.01 – 8.27 3.68 – 11.32 㔤⭏㍐ E[–⭏㛢䞊] 5.96 – 18.44a; 2.83 – 15.53b 1.5 – 68.7 ㊭㋂ᣇ㩕ޫഐᆀਜ਼䟿˄ᒢส%˅ Ἵ䞨 0.69 – 1.09a; 0.58 – 0.97b 0.111 – 1.570 ỹᆀ㌆ 0.079 – 0.22a; 0.028 – 0.15b 0.020 – 0.320 ㊭㋂⅑⭏ԓ䉒ӗ⢙˄g/g dwt˅ 123 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ㊭㋂㓴㓷ᡀ࠶ 1 ਁ㺘᮷⥞ѝᮠ٬㤳ത 2 ILSI ᮠᦞᓃѝᮠ٬㤳ത 3 䱯兿䞨 1205.75 – 2873.05a; 820.14 – 2539.86b 291.9 – 3885.8 ሩ-俉䉶䞨 94.77 – 327.39a; 64.03 – 259.68b 53.4 – 576.2 ൠк䜘ἽṚ㩕ޫᡀ࠶ ᑨ㿴ᡀ࠶˄ᒢส%˅ ⚠࠶ 2.67–8.01a; 3.88 – 6.90b 1.527 – 9.638 ᣈਸ⻣≤ॆਸ⢙ 81.88 – 89.26a; 84.11 – 89.52b 76.4 – 92.1 ᙫ㜲㛚 1.28 – 3.62a; 0.20 – 2.33b 0.296 – 4.570 ≤࠶˄⒯ส%˅ 64.20 – 75.70a ; 71.40 – 78.00b 49.1 – 81.3 㳻ⲭ䍘 5.80 – 10.24a; 5.56 – 9.14b 3.14 – 11.57 㓔㔤˄ᒢส%˅ 䞨ᙗ⍇⏔㓔㔤˄ADF˅ 19.11 – 30.49a; 20.73 – 33.39b 16.13 – 47.39 ѝᙗ⍇⏔㓔㔤˄NDF˅ 27.73 – 49.62a; 31.81 – 50.61b 20.29 – 63.71 ⸯ⢙䍘˄ᒢส%˅ 䫉 0.12 – 0.33a; 0.17 – 0.41b 0.0714 – 0.5768 ⼧ 0.090 – 0.26a; 0.13 – 0.21b 0.0936 – 0.3704 1 dwt = ᒢ⢙䟽DŽ 2 ਁ㺘᮷⥞ᶕⓀ˖a (Harrigan et al., 2009) [US 2006], b (Harrigan et al., 2009) [Chile 2006/2007]. 3 ILSI ᮠᦞᶕⓀ˖ILSI ⢙ᡀ࠶ᮠᦞᓃ˄Version 3.0˅ˈhttp://www.cropcomposition.org/ (ILSI, 2006) 124 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.3.5 ᣍ⭕㍖ᣍᙝ MON 87427 Ⲵᨂޕᒿࡇ˄T-DNA˅ѝнਜ਼ᴹԫօᣇ⭏㍐ᣇᙗสഐ˄മ 6˅DŽ MON 87427 Ⲵ䖜ॆ㺘䗮䖭փާᴹⲴ aadAˈ൘བྷ㛐ᵶ㧼઼ߌᵶ㧼࠶ᆀݻ䲶 䗷〻ѝ⭘ᣇ⭏㍐䘹ᤙḷ䇠˄Fling et al., 1985˅DŽ⭡Ҿ aadA ൘䖜ॆ⡷⇥˄T-DNA˅ 䗩⭼ᒿࡇѻཆˈഐ↔⋑ᴹ㻛䖜ॆࡠἽ⢙สഐ㓴DŽ䙊䗷 Southern ᵲӔҏ䇱ᇎҶ MON 87427 нਜ਼䍘㋂僘ᷦᒿࡇ˄മ 17˅DŽ 3.3.6 ሯӰ㊱ૂ伕ᆿޞᙝⲺެᆹᖧଃ ⭏⢙ᢰᵟ⢙ѝሬޕⲴ㳻ⲭሩӪ㊫伏⭘Ⲵᆹޘᙗ䇴ՠᗵ享㘳㲁ԕлࠐњ ᓄ⭘শਢ˗(c) оᐢ⸕ޘᙗ˗(b) 伏૱ᓄ⭘৺䮯ᵏᆹޘᯩ䶒˖(a) փ⭏⢙Ⲵᆹ ∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃ㠤㳻ⲭ઼ަᆳሩପңࣘ⢙ާᴹн㢟⭘Ⲵ⍫ᙗ㳻ⲭ ൘㔃ᶴкⲴլᙗ˗(d) ࣐✝༴⨶ਾⲴっᇊᙗ˗(e) ൘⁑ᤏ㛳⏢ѝⲴ⎸ॆᙗ˗ (f) ሩପңࣘ⢙Ⲵ▌൘∂ᙗDŽԕкⲴᆹޘ䇴ԧ䍴ᯉᐢ㓿ሩ䘉Ӌᯩ䶒䘋㹼Ҷ䈖㓶䱀 䘠ˈMON 87427 ⦹㊣ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭо䇨ཊᐢ㓿୶ъॆⲴᣇ㥹⭈㟖 ⢙ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭаṧ˗൘䗷৫ 20 ᒤˈޘ⨳ᐢ㓿ᴹ 488 亩⅑ޣҾ CP4 EPSPS 㳻ⲭᆹޘᙗⲴᇑḕᡆ䇴ᇑˈᗇࠪⲴ㔃䇪൷䇔Ѫ CP4 EPSPS 㳻ⲭሩӪ ᓧ⋑ᴹн㢟ᖡ૽DŽڕ⢙㊫઼ࣘ ⭡Ҿ MON 87427 ⦹㊣ᱟᣇ䲔㥹ࡲ⢙ˈ൘⭏ӗ֯⭘䗷〻ѝ䴰㾱䝽ਸ֯⭘ ԕ㥹⭈㟖Ѫᴹ᭸ᡀ࠶Ⲵ䲔㥹ࡲDŽ൘ᯠⲴᣇ䲔㥹ࡲᙗ⣦୶ъॆἽѻࡽˈ䜭Պ᤹ ➗㢟ྭߌъ㿴㤳˄Good Agricultural Practices ˈGAP˅ḷ߶䘋㹼⭠䰤䈅傼ˈ᤹ ➗᧘㦀ࡲ䟿઼ᯩ⌅൘⢙кⴤ᧕௧ᯭ䲔㥹ࡲˈ࠶᷀⺞ᇊⴤ᧕᭦㧧Ⲵߌӗ૱৺ަ ࣐ᐕ㓴࠶ѝⲴ䲔㥹ࡲ↻⮉≤ᒣDŽ䲔㥹ࡲ൘㊭㋂н਼㓴࠶ѝ↻⮉≤ᒣਟ㜭ᆈ൘ᐞ ᔲˈ൘ḀӋ㓴࠶ѝሩᇼ䳶ˈ㘼൘ަᆳ㓴࠶ѝሩ䖳ቁDŽ࣐ᐕ㓴࠶ѝⲴሩ↻ ⮉⎃㕙≤ᒣ⭘⎃㕙㌫ᮠ˄concentration factors, CF˅㺘⽪ˈ䇑㇇ޜᔿྲл˖CF= ࣐ᐕ㓴࠶↻⮉≤ᒣ/ᵚ࣐ᐕ㊭㋂↻⮉≤ᒣDŽ MON 87427 Ⲵ䳴ᙗಘᇈѝ CP4 EPSPS 㳻ⲭ㺘䗮䟿ᶱվˈн㜭㙀ਇ㥹⭈㟖䲔 㥹ࡲDŽ൘⦹㊣ᵲӔࡦ䗷〻ѝˈ⭘ਜ਼ᴹ MON 87427 䖜ॆփⲴ⦹㊣㠚Ӕ㌫⇽ ᵜˈُࣙҾ㥹⭈㟖䲔㥹ࡲⲴ௧ᯭӗ⭏䳴ᙗн㛢ˈ⭘ਜ਼ᴹ NK603ǃMON 88017 ㅹ ަᆳᣇ䲔㥹ࡲ䖜ॆփⲴ㠚Ӕ㌫⡦ᵜˈ⭏ӗⲴᵲӔ MON 87427 × NK603 ᡆ ❦MON 87427 × MON 88017 ⭘Ҿ⭏ӗ䬰DŽ䘉ӋᵲӔⲴṭษ䗷〻ѝˈߌ≁ Պ᤹➗਼Ⲵ㥹⭈㟖ḷㆮ᧘㦀ᯩᔿ௧ᯭ䲔㥹ࡲDŽ 2008 ᒤ൘㖾ഭ⦹㊣Ἵ४ 6 њᐎǃ6 њ䈅傼⛩ሩਜ਼ᴹ MON 87427 䖜ॆһԦ Ⲵ⦹㊣ᵲӔ MON 87427 × NK603 䘋㹼⭠䰤䈅傼ˈ᤹➗ḷㆮ᧘㦀֯⭘ᯩ⌅௧ᯭ 㥹⭈㟖ˈỰ⍻࠶᷀Ҷ᭦㧧Ⲵ⦹㊣㊭㋂ѝ㥹⭈㟖৺ަԓ䉒ӗ⢙≘⭢ส⼧䞨˄AMPA˅ Ⲵ↻⮉≤ᒣ˄㿱ᢰᵟᣕ 16a˅ˈഭ䱵伏૱⌅ިငઈՊ˄Codex˅൘⦹㊣кᡰ䇮 ᇊⲴ㥹⭈㟖ᴰབྷ↻⮉䲀䟿˄MRL˅ਚवᤜ㥹⭈㟖DŽк䘠 MON 87427 ᵲӔ㥹⭈ 㟖ⲴỰ⍻㔃᷌㿱л㺘ˈ㺘ѝ MON 87427 ᵲӔ⦹㊣㥹⭈㟖↻⮉䟿ⲴỰ⍻㔃᷌о ࡙⭘ㅜањᣇ㥹⭈㟖⦹㊣ GA21 ᔪ・Ⲵสᮠᦞสᵜа㠤ˈ䜭վҾ Codex MRL 125 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ٬DŽ㘼สҾ GA21 䘋㹼Ⲵ࣐ᐕ㓴࠶↻⮉⹄ウᔪ・Ҷ䈕㊫⦹㊣⋩ǃ⦹㊣㊹Ⲵ↻⮉ᮠ ᦞˈࡇҾл㺘DŽ 2016 ᒤ 4 ᴸˈ൘㖾ഭሩ MON 87427 × NK603 ⦹㊣˄㥹⭈㟖௧ᯭ༴⨶˅઼ᑨ 㿴⦹㊣ MPA640B˄ᵚ௧ᯭ༴⨶˅䘋㹼Ҷ䲔㥹ࡲ↻⮉≤ᒣⲴ࠶᷀ˈੁࠪ㤇ਾⲴ⦹ ㊣௧ᯭ䗷䟿˄~2×˅Ⲵ㥹⭈㟖䲔㥹ࡲ MON 79789ˈᴰѪ V8 ᵏᡆṚ儈 48 㤡ረDŽ MON 79789 Ⲵ௧ᯭᙫࡲ䟿Ѫ 4.5 lb a.e./㤡әˈ㘼㖾ഭሩਜ਼ᴹ NK603 ᡀ࠶ⲴᵲӔ ㊣ࡦᇊⲴᴰབྷݱ䇨㤇ਾ௧ᯭࡲ䟿Ѫ 2.25 lb a.e./ 㤡әDŽ᭦䳶ࡠⲴ⦹㊣ἂ⭡㚄ਸ⦹ ᭦ࢢᵪ㝡㋂ˈᡰᗇ㊭㋂㖞Ҿ 4 њ㺻ᆀѝˈ⇿㺻㓖 50 ⦹㊣㊭㋂DŽ㊭㋂㓿䗷⌱ǃ 䘹ਾ࠶ᡀࠐ㓴ԕ༷⹄⼘࣐ᐕˈሶ⌱ਾⲴᆼᮤ㊭㋂䘋㹼≤࠶䈳㢲ˈ㠣ਜ਼≤䟿㓖 21%DŽ⭘ⴈᔿ⼘㊹ᵪ㊹⺾⦹㊣㋂ˈ᭦䳶⼘ࠪⲴᒢ㊹ˈ㖞Ҿߧ߫㇡ѝԕ༷࠶᷀DŽ㜊 ң䜘࠶㓿仾ᒢǃ࣐✝ǃ⡷ǃ└ㆋㅹˈԕᐢ✧㨳ਆˈӾ㜊㣭㊹ѝ࠶ࠪ㋇ᨀ ⦹㊣⋩DŽ㋇ᨀ⋩㓿㋮ࡦǃ┲ⲭ઼৫㠝˄RBD˅༴⨶ˈᗇࡠ㋮⛬ RBD ⦹㊣⋩ˈ㖞 Ҿߧ߫㇡ѝԕ༷࠶᷀DŽ࡙⭘ᑖᴹ⭥ᆀॆ௧䴮ಘⲴ LC-MS/MS ㌫㔏⍻ᇊࡍ⦹ ㊣㊭㋂৺ަ࣐ᐕ㓴࠶ѝⲴ㥹⭈㟖઼ AMPA ↻⮉≤ᒣDŽ࠶᷀㔃᷌㿱л㺘ˈ䈖㓶⹄ ウᣕ䈧㿱ᵜ⭣䈧䍴ᯉлᢰᵟᣕ 16bDŽ ഭ䱵伏૱⌅ިငઈՊ˄Codex˅൘⦹㊣кᡰ䇮ᇊⲴ㥹⭈㟖ᴰབྷ↻⮉䲀䟿˄MRL˅ ਚवᤜ㥹⭈㟖DŽк䘠 MON 87427 × NK603 ᵲӔ⦹㊣ѝ㥹⭈㟖ⲴỰ⍻㔃᷌㿱л 㺘ˈ㺘ѝ MON 87427 ᵲӔ⦹㊣㥹⭈㟖↻⮉䟿ⲴỰ⍻㔃᷌䜭䘌վҾ Codex MRL ⦹٬DŽ㺘ѝഭ䱵伏૱⌅ިငઈՊ˄Codex˅ᡰ䇮・Ⲵᴰབྷ↻⮉䲀䟿ᴰࡍᱟ൘ᑨ㿴 ㊣к䇮ᇊⲴDŽ൘ᣇ㥹⭈㟖⦹㊣кⲴ↻⮉䈅傼㔃᷌㺘᰾ˈ䲔㥹ࡲ㥹⭈㟖Ⲵ↻⮉≤ᒣ վҾ Codex 䇮ᇊⲴ MRLDŽഐ↔⧠ᴹⲴ Codex MRL ᰒ䘲⭘Ҿᑨ㿴⦹㊣ˈҏ䘲⭘ Ҿᣇ㥹⭈㟖䲔㥹ࡲ⦹㊣DŽྲ᷌⦹㊣࣐ᐕ㓴࠶Ự⍻ࡠⲴ↻⮉䟿≤ᒣ᰾ᱮ儈Ҿ㊭㋂к ٬DŽն⭡Ҿ⦹㊣ Codex ᡆ䇨Պ䪸ሩ࣐ᐕ㓴࠶䇮ᇊу䰘Ⲵ MRLˈ٬ 䇮ᇊⲴ MRL ˈ˅5 ppm˄٬ ǃ⦹㊣㊹ㅹ㓴࠶ѝỰ⍻ࡠⲴ㥹⭈㟖Ⲵ↻⮉䟿䘌վҾ㊭㋂кⲴ MRL⋨ ٬DŽ ഐ↔⋑ᴹу䰘䇮・⦹㊣㊹ǃ⦹㊣⋩Ⲵ MRL ߌ㦟ᴰབྷ↻⮉䲀䟿˄MRL˅ᱟᤷ伏૱ᡆߌӗ૱䜘ᡆ㺘䶒⌅ᇊݱ䇨Ⲵߌ㦟 ᴰབྷ⎃ᓖˈԕ⇿ॳݻ伏૱ᡆߌӗ૱ѝߌ㦟↻⮉Ⲵ∛ݻᮠ㺘⽪˄mg/kg ᡆ ppm˅DŽ ߌ㦟ᴰབྷ↻⮉䲀䟿˄MRL˅ᱟ䙊䗷ሩߌ㦟䘋㹼ᒯ⌋Ⲵ∂⨶ᆖ䈅傼઼ሩҾᴍ䵢 ᓧӗ⭏ڕ䇱нՊሩӪ㊫؍䟿Ⲵ㟣伏仾䲙䇴ՠˈ⺞ᇊⲴਟ᧕ਇⲴߌ㦟↻⮉≤ᒣˈԕ н㢟ᖡ૽DŽߌ㦟ᴰབྷ↻⮉䲀䟿ᇎ䱵кᱟߌ㦟ਸ⨶֯⭘઼ߌӗ૱䍨᱃ᡰ䴰㾱䚥ӾⲴ ḷ߶DŽᴹӋഭᇦᦞ⌅ᖻ⌅㿴Ⲵ㾱≲ˈՊࡦᇊᵜഭߌӗ૱ᴰབྷ↻⮉䲀䟿ḷ߶ˈ㘼 Codex MRL Ѫഭ䱵伏૱⌅ިငઈՊ伏૱ḷ߶Ⲵа䜘࠶ˈ⭡ Codex ߌ㦟↻⮉င ઈՊᦞ FAO/WHO ߌ㦟↻⮉㚄ᑝՊ䇞˄JMPR˅Ⲵᔪ䇞ࡦᇊⲴDŽJMPR ᱟ⭡㚄 ਸഭ㋞ߌ㓴㓷઼ц⭼ছ⭏㓴㓷уᇦޡ਼㓴ᡀⲴ、ᆖ亮䰞㓴㓷ˈJMPR 䙊䗷ሩߌ㦟 ˅ᆖᮠᦞ઼↻⮉ᮠᦞ䘋㹼䇴ԧˈᦞ䇴ԧ㔃䇪ˈੁഭ䱵伏૱⌅ިငઈՊ˄Codex⨶∂ ᔪ䇞ᴰབྷ↻⮉䲀䟿ḷ߶˄MRL˅DŽ ᙫѻˈ䙊䗷↻⮉䈅傼ˈ࠶᷀Ự⍻Ҷ MON 87427 × NK603 ⦹㊣ᵲӔᡰ᭦ 㧧㊭㋂Ⲵ㥹⭈㟖↻⮉≤ᒣDŽ൘㊭㋂ѝỰ⍻ࡠⲴᴰ儈↻⮉䟿䘌վҾ Codex ൘ᑨ㿴 ⦹㊣к䇮・Ⲵᴰབྷ↻⮉䲀䟿≤ᒣDŽᣇ䲔㥹ࡲ⦹㊣㊭㋂৺ަ࣐ᐕᡀ࠶㥹⭈㟖↻⮉ ᮠᦞ㺘᰾ˈሩҾ⦹㊣㊭㋂ˈ⦹㊣⋩ѝⲴ㥹⭈㟖↻⮉≤ᒣᶱབྷ䱽վˈ⦹㊣㊹↻ 126 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⮉≤ᒣ∄㊭㋂ҏ〽ᴹ߿ቁDŽᰐ䇪ᱟ⦹㊣㊹ǃ⦹㊣⋩䘈ᱟ㊭㋂ᵜ䓛ˈ㥹⭈㟖↻ ٬DŽ ⮉䟿䜭䘌վҾ Codex ൘⦹㊣㊭㋂к䇮ᇊⲴ MRL 㺘 29. ᣇ㥹⭈㟖⦹㊣↻⮉䈅傼ᙫ㔃৺ Codex ᴰབྷ↻⮉䲀䟿≤ᒣ˄MRL˅ 㥹⭈㟖 GA21 ᵲӔ 1 MON 87427 × NK603 ᵲӔ 2 㓴㓷㊫ර Codex MRL ѝսᮠ˄㤳ത˅ ѝսᮠ˄㤳ത˅ ppm ppm ppm 0.11 0.04 ⦹㊣㊭㋂ 5 (<0.05 - 0.96) (0.01 - 0.13) <0.04 <0.053 ㋮⛬⦹㊣⋩ ᵚ䇮ᇊ (<0.02 – 0.32) (<0.05 - 0.1) 0.12 <0.054 ⦹㊣㊹ ᵚ䇮ᇊ (<0.05 – 1.1) (<0.05 - <0.05) 1 ਜ਼ᴹ䖜ॆһԦ GA21 Ⲵㅜаԓᣇ㥹⭈㟖⦹㊣ᵲӔⲴ㥹⭈㟖↻⮉ᮠᦞˈ⭘Ҿᔪ・䈕㊫ ᣇ㥹⭈㟖⦹㊣᭦㧧ӗ૱઼᭦㧧㊭㋂࣐ᐕ㓴࠶↻⮉สᮠᦞ˗ 2 ㊭㋂↻⮉ᮠᦞᶕ㠚Ҿ 2008 ᒤ㖾ഭޝњ䈅傼⛩ѝԕ㥹⭈㟖ḷㆮࡲ䟿௧ᯭⲴ↻⮉䈅傼˄ᢰ ᵟᣕ 16a˅ˈ㋮⛬⦹㊣⋩઼⦹㊣㊹ѝⲴ㥹⭈㟖↻⮉≤ᒣ䙊䗷 2016 ᒤ⍻ᗇⲴ⎃㕙㌫ᮠᣈ ㇇ᗇࡠ˄ᢰᵟᣕ 16b˅˗ ⎃㕙㌫ᮠ=[࣐ᐕ㓴࠶ѝ࠶᷀⢙Ⲵ⎃ᓖ]/[RAC ㊭㋂ѝ࠶᷀⢙ Ⲵ⎃ᓖ]DŽྲ᷌Ḁ࣐ᐕ㓴࠶ѝⲴ↻⮉≤ᒣվҾ LOQ˄<0.05 ppm˅ᡆ㘵ᵚỰ⍻ࡠ࠶᷀⢙ˈ ࡉ⭘<0.05 ppm Ⲵ↻⮉≤ᒣᶕ䇑㇇䈕㓴࠶Ⲵ⎃㕙㌫ᮠDŽ⭡Ҿᮠᆖ䇑㇇Ⲵഋ㠽ӄޕ㿴ࡉˈ DŽޕᴹࠪ〽㺘ѝ⧠Ⲵ⎃㕙㌫ᮠਟ㜭Պоࣘ䇑㇇٬ 3 ⦹㊣⋩⎃㕙㌫ᮠ=⦹㊣⋩↻⮉䟿/⦹㊣㊭㋂↻⮉䟿=<0.05ppm/0.91ppm=<0.06˄⎃㕙㌫ᮠ ˅ᇊ٬䈧㿱ᢰᵟᣕ 16b⍻ 4 ⦹㊣㊹⎃㕙㌫ᮠ=⦹㊣㊹↻⮉䟿/⦹㊣㊭㋂↻⮉䟿=0.83ppm/0.91ppm=0.91˄⎃㕙㌫ᮠ⍻ ˅ᇊ٬䈧㿱ᢰᵟᣕ 16b 3.4 ṯᦤр䘦䇺ԭθ৸➝ᵢࣔ⌋ㅢॷпᶗᴿީḽࡈ࠼䖢ะഖὃ⢟ Ⲻᆿޞㅿ㓝 MON 87427㌫䟷⭘ᆹޘㅹ㓗ѪIⲴᑨ㿴⦹㊣ᵲӔѪਇփᒦ㓿㊫ර2Ⲵสഐ ᓧԕ৺ሩ⭏ڕ⢙ᯩ⌅䖜ॆਾ㧧ᗇⲴDŽབྷ䟿Ⲵ⹄ウ㺘᰾MON 87427ሩӪ㊫઼ࣘ ᘱ⧟ຳᆹޘᙗⲴᖡ૽оᑨ㿴䶎䖜สഐ⦹㊣ᱟа㠤ⲴDŽṩᦞljߌъ䖜สഐ⭏⢙ᆹޘ 䇴ԧ㇑⨶࣎⌅NJㅜҼㄐㅜॱйᶑⲴ㿴ᇊˈ䖜สഐἽ⢙ᓄ䈕Ҿᆹޘㅹ㓗IDŽ 127 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 4. 䖢ะഖὃ⢟ӝᆿޞᙝ䇺ԭ 4.1 ⭕ӝૂࣖᐛ⍱ࣞሯ䖢ะഖὃ⢟ⲺᆿޞᙝⲺᖧଃ ൘⦹㊣㊭㋂ԕ৺ਜ਼ᴹ⦹㊣㊭㋂Ⲵӗ૱Ⲵ࣐ᐕ䗷〻ѝˈሩሬޕ㳻ⲭⲴᆹޘᙗᖡ ૽ᴰབྷቡᱟ࣐✝༴⨶DŽᇎ䱵кˈ࣐✝༴⨶൘⦹㊣㊭㋂৺ަӗ૱Ⲵ࣐ᐕ䗷〻ѝ䶎ᑨ Პ䙽DŽഐ↔ˈ䇴ԧ࣐✝༴⨶ሩ CP4 EPSPS 㳻ⲭっᇊᙗⲴᖡ૽ᱟ䶎ᑨ䟽㾱ⲴDŽ ྲ 3.3.2 䜘࠶ᡰ䘠ˈ䞦⍫ᙗᇎ傼㔃᷌㺘᰾ˈԕ 25ºCǃ37ºC ઼ 55ºC ࣐✝ 15 min ਾ㿲ሏࡠ CP4 EPSPS ⍫ᙗ〽ᴹ䱽վˈ࠶࡛䱽㠣ሩ➗Ⲵ 81%ǃ84%઼ 70%˗ն࣐ ✝ 30 min ਾˈ25ºC ༴⨶Ⲵ CP4 EPSPS ⍫ᙗ⋑ᴹਈॆˈ37ºC ༴⨶㓴Ⲵ䞦⍫䱽㠣ሩ ➗ⲴⲴ 88%ˈ㘼 55ºC ༴⨶㓴Ⲵ CP4 EPSPS 㺘⧠Ѫབྷᑵл䱽ˈӵѪሩ➗Ⲵ 25%DŽ н䇪ᱟ༴⨶ 15 min 䘈ᱟ༴⨶ 30 minˈ75°C ઼ 95°C ᓖᶑԦлⲴ CP4 EPSPS 䞦 ⍫൷վҾỰ⍻䲀˄㺘 19 ઼㺘 20˅DŽSDS-PAGE ࠶᷀㔃᷌㺘᰾ˈ৲➗ḷ߶઼ሩ➗ ⢙वਜ਼аᶑ㺘㿲࠶ᆀ䟿㓖Ѫ 43.8 kDa ⲴѫᶑᑖˈሩᓄҾ CP4 EPSPS 㳻ⲭ˄മ 23 ⋐മ 24˅DŽ൘ᡰᴹ༴⨶ᰦ䰤઼༴⨶ᓖᶑԦлˈṧ૱Ⲵ 43.8 kDa ᶑᑖؑਧᕪᓖ઼ ᴹ᰾ᱮⲴ䱽վ˄മ 23ˈ⌣䚃 3~7˗മ 24ˈ⌣䚃 2~7˅DŽ ഐ↔ˈ࣐✝༴⨶ӵՊ֯ CP4 EPSPS 㳻ⲭⲴ䞦⍫ᙗ䱽վˈնнՊ֯ CP4 EPSPS 㳻ⲭ䱽䀓ˈнՊᖒᡀᯠⲴ㳻ⲭ⡷⇥㘼ᑖᶕᯠⲴ▌൘㠤仾䲙DŽ ൘ަᆳᯩ䶒ˈ⭏ӗ઼࣐ᐕ⍫ࣘሩ MON 87427 ⦹㊣ӗ૱઼ᑨ㿴⦹㊣ӗ૱Ⲵᖡ ૽ᱟ਼ⲴDŽ࣐✝༴⨶൘аᇊ〻ᓖкՊ֯ᣇ㩕ޫഐᆀཡ৫⍫ᙗˈഐ↔Պᨀ儈䖜ส ഐἽ⢙࣐ᐕӗ૱ⲴᆹޘᙗDŽ 4.2 䖢ะഖὃ⢟ӝⲺどᇐᙝ оᑨ㿴⦹㊣∄ˈMON 87427 ⦹㊣ѝሬޕањᶕⓀҾ൏༔ߌᵶ㧼Ⲵ cp4 epsps สഐ㺘䗮ⴂˈਟԕ㺘䗮 CP4 EPSPS 㳻ⲭDŽ䲔↔ཆˈMON 87427 оᑨ㿴 ⦹㊣⋑ᴹӰѸн਼DŽCP4 EPSPS 㳻ⲭᱟ㣣俉᯿≘ส䞨ਸᡀ䙄ᖴкⲴањ䞦㳻ⲭˈ о MON 87427 ࣐ᐕӗ૱Ⲵっᇊᙗ⋑ᴹޣ㌫DŽਖཆˈCP4 EPSPS 㳻ⲭ൘࣐ᐕⲴ✝ ༴⨶䗷〻ѝՊཡ৫⍫ᙗ˄㿱к䘠 4.1 䜘࠶˅DŽഐ↔ˈMON 87427 ࣐ᐕӗ૱Ⲵっᇊ ᙗоᑨ㿴⦹㊣࣐ᐕӗ૱Ⲵっᇊᙗ⋑ᴹᐞᔲDŽ о MON 87427 ⦹㊣аṧˈMON 87427 Ⲵ࣐ᐕӗ૱ѝҏवਜ਼ CP4 EPSPS 㳻 ⲭDŽ⁑ᤏ㛳⏢⎸ॆᇎ傼㔃᷌㺘᰾ˈ൘⁑ᤏ㛳⏢ᶑԦл 15 s ਾ CP4 EPSPS 㳻ⲭՊ 㻛⎸ॆ 95%ԕкˈ䞦⍫ᙗՊ䱽վ 90%ԕк˄㿱 3.3.2 䜘࠶˅DŽ 4.3 䖢ะഖὃ⢟ӝૂ䖢ะഖὃ⢟ҁ䰪⧥ູᆿޞᙝⲺᐤᔸ MON 87427 ⦹㊣઼ᑨ㿴⦹㊣ѻ䰤ୟаⲴᐞ࡛൘Ҿ MON 87427 ѝሬޕ cp4 epsps สഐˈਟԕ㺘䗮ཆⓀ CP4 EPSP 㳻ⲭˈ֯ MON 87427 ਟԕᣇ㥹⭈㟖䲔 㥹ࡲDŽնབྷ䟿Ⲵᆹޘ䇴ԧ⹄ウ㔃᷌㺘᰾˖1˅MON 87427 ѝⲴཆⓀสഐᨂޕѪ অᤧ䍍ᨂޕˈਟԕっᇊ䚇Ր˗2˅CP4 EPSPS 㳻ⲭнՊሩ⧟ຳᑖᶕн࡙ᖡ૽˗3˅ MON 87427 Ⲵ㺘රᙗ⣦઼ߌ㢪ᙗ⣦оᑨ㿴⦹㊣⋑ᴹᱮ㪇ᐞᔲˈнՊ໎࣐䖜ਈѪ 128 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᴹᇣ⭏⢙Ⲵ仾䲙˗4˅ሩ䶎䶦ḷ⭏⢙Ⲵᖡ૽ਟԕᘭ⮕н䇑ˈнՊᖡ૽⧽〰઼☂ড ⢙DŽഐ↔ਟԕ䈤ሬޕⲴཆⓀ㳻ⲭሩ⧟ຳᆹޘᙗнՊӗ⭏н࡙ᖡ૽DŽ৽ˈ MON 87427 Ⲵᣇ䲔㥹ࡲ⢩ᙗ֯ߌ≁ᴹҶᴤཊᵲ㥹᧗ࡦ઼㙅ᯩᔿⲴ䘹ᤙˈਟԕ ⧟ᣔ؍㲰ˈᴹ࡙Ҿᴤྭൠץ䘋ݽ㙅ᢰᵟⲴਁኅˈᴹ࡙Ҿ߿ቁ≤൏⍱ཡ઼൏༔׳ ຳDŽ MON 87427 ӗ૱൘⧟ຳᆹޘᙗᯩ䶒о MON 87427 ⋑ᴹӰѸн਼DŽն MON 87427 ࣐ᐕӗ૱ѝ CP4 EPSPS 㳻ⲭ⍫ᙗՊ䱽վᡆїཡˈ਼ᰦ࣐ᐕӗ૱䟺 ᭮ࡠ⧟ຳⲴਟ㜭ᙗՊᴤվDŽ 㔬кᡰ䘠ˈMON 87427 ⴤ᧕᭦㧧ӗ૱ᡆ㘵ަ࣐ᐕӗ૱䜭нՊ᭩ਈަሩ⧟ຳ ᆹޘᙗⲴᖡ૽ˈо MON 87427 ᵜ䓛ሩ⧟ຳᆹޘⲴᖡ૽ᱟаṧⲴDŽ ᓭᖧଃⲺᐤᔸڛ㊱䖢ะഖὃ⢟ӝૂ䖢ะഖὃ⢟ҁ䰪ሯӰ 4.4 ྲࡽᡰ䘠ˈMON 87427 䖜ॆփ઼ᑨ㿴⦹㊣䰤Ⲵୟаᐞ࡛൘Ҿ MON 87427 ѝ cp4 epsps สഐˈਟԕ㺘䗮ཆⓀ CP4 EPSP 㳻ⲭˈ֯ MON 87427 ਟԕᣇ㥹⭈ ޕሬ 㟖䲔㥹ࡲDŽ䲔Ҷᣇ䲔㥹ࡲᙗ⣦ˈMON 87427 ൘ߌ㢪ᙗ⣦ǃ㺘⧠රᙗ⣦઼㩕ޫᡀ ࠶ㅹᯩ䶒оᑨ㿴⦹㊣⋑ᴹᱮ㪇ᐞ࡛DŽབྷ䟿Ⲵᆹޘ䇴ԧ⹄ウ㔃᷌㺘᰾˖1˅⦹㊣ᱟ 䮯ᵏ傟ॆ⢙ˈѪӪԜᡰ⟏⸕ˈާᴹ䮯ᵏᆹޘᓄ⭘ⲴশਢˈMON 87427 оѻާ ᴹᇎ䍘ㅹ਼ᙗ˗2˅MON 87427 ѝཆⓀᨂޕᒿࡇѪঅᤧ䍍Ⲵᆼᮤᨂޕˈਟԕっᇊ 䚇Ր˗3˅CP4 EPSPS 㳻ⲭᱟἽ⢙઼ᗞ⭏⢙ѝᲞ䙽ᆈ൘Ⲵ EPSPS 㳻ⲭѝⲴаˈ 䶎ᑨլ˗4˅CP4 EPSPS оᐢ⸕Ⲵ∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃ㠤㳻ⲭ൘ᒿࡇ઼ 㔃ᶴкнާլᙗ˗5˅ሿ啐ᙕᙗ∂ᙗᇎ傼㔃᷌㺘᰾ CP4 EPSPS 㳻ⲭнާᴹ∂ᙗˈ NOAEL Ѫ㠣ቁ 2000 mg/kg/ཙˈMOE 儈䗮 2.76 × 106˗6˅൘⁑ᤏ㛳⏢ᶑԦл 15 s ਾ 95%ԕкⲴ CP4 EPSPS 㳻ⲭՊ㻛⎸ॆᦹ˗7˅࣐✝༴⨶Պ֯ CP4 EPSPS 㳻ⲭ ཡ⍫նнՊ䱽䀓˗8˅MON 87427 оᑨ㿴⦹㊣൘㩕ޫᡀ࠶кᇎ䍘ㅹ਼˗9˅ MON 87427 ѝ CP4 EPSPS 㳻ⲭоަᆳᐢ㓿ᢩ߶୶ъॆᓄ⭘Ⲵ䇨ཊަᆳᣇ㥹⭈㟖 ⢙˄ྲ NK603 ⦹㊣ǃ40-3-2 བྷ䉶઼ MON89788 བྷ䉶ㅹ˅Ⲵ CP4 EPSPS 㳻ⲭ ᓧᑖᶕ仾䲙DŽڕDŽഐ↔ˈMON 87427 нՊሩӪ㊫਼ MON 87427 ӗ૱Ⲵ࣐ᐕ䗷〻ਟ㜭ᆈ൘࣐✝༴⨶DŽ࣐✝༴⨶Պሬ㠤 CP4 EPSPS 㳻ⲭཡ⍫ˈഐ↔нՊ໎࣐ᡆ᭩ਈ MON 87427 ӗ૱ⲴᆹޘᙗDŽ ᓧڕᙫѻˈMON 87427 ⴤ᧕᭦㧧ӗ૱ᡆ㘵ަ࣐ᐕӗ૱䜭нՊ᭩ਈަሩӪ㊫ ᓧⲴᖡ૽ᱟаṧⲴDŽڕᙗˈо MON 87427 ᵜ䓛ሩӪ㊫ޘⲴᆹ 4.5 ৸➝ᵢࣔ⌋ㅢॷᶗᴿީḽࡈ࠼䖢ะഖὃ⢟ӝⲺᆿޞㅿ 㓝 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Journal of Molecular and Applied Genetics 1:361-370. 140 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ޝǃޣ䱴Ԧ䍴ᯉ ީ䱺Ԭ䍺ᯏⴤᖋ ᓅਭ 䱺Ԭ䍺ᯏ 享⸷ 1 ⴤⲺะഖⲺṮ㤭䞮ᓅࡍެ᧞ሲⲺ≞ะ䞮ᓅࡍ 142 2 ⴤⲺะഖф䖳։ᶺᔰⲺഴ䉧 143 3 ⴤⲺะഖфὃ⢟ะഖ㓺᮪ਾެ㺞䗴Ⲻ࠼ᆆỶ⎁ᡌ䢪ᇐ㔉 146 ᷒δPCR Ỷ⎁ȽSouthern ᵸӚ࠼᷆ȽNorthern ᡌ Western ࠼᷆㔉᷒ȽⴤⲺะഖӝ⢟㺞䗴㔉᷒ε 4 䖢ะഖᙝ⣬ӝ⢟ⲺỶ⎁ૂ䢪ᇐᢶᵥ 171 5 䈋僂䱬⇫ᇗҜⲺགྷদԬδ䘑ਙ䖢ะഖ⭕⢟⭩䈭ᆿ 174 ޞ䇷ҜⲺθᵢ亯уມߏε 6 䈋僂䱬⇫Ⲻᆿޞᙝ䇺ԭ䈋僂ᙱ㔉ᣛδ䘑ਙ䖢ะഖ⭕⢟ 174 ⭩䈭ᆿޞ䇷ҜⲺθᵢ亯уມߏε 7 䖢ะഖὃ⢟ሯ⭕ᘷ⧥ູᆿޞᙝⲺ㔲ਾ䇺ԭᣛ 174 8 伕⭞ᆿޞᙝⲺ㔲ਾ䇺ԭᣛθऻᤢ:Aεᗻ㾷Ⲻࣞ⢟∈⨼䈋 174 僂ᣛχBε伕䗽ᮅᙝ䇺ԭ䈋僂ᣛχCεф䶔䖢ะഖὃ ⢟∊䖹θެ㩛ޱᡆԳᣍ㩛ޱഖᆆ࠼᷆ᣛㅿ 9 䈛㊱䖢ะഖὃ⢟ളཌ⭕ӝᓊ⭞Ᾰ߫ 182 10 ⭦䰪ⴇᯯṾθऻᤢⴇᢶᵥȽᣍᙝ⋱⨼᧠᯳Ƚ䮵ᵕ⧥ູ 268 ᭾ᓊⲺ⹊ガᯯ⌋ㅿ 11 ᇗḛᡶ䴶Ⲻެᆹީ䍺ᯏ 269 䖢ะഖ⭕⢟൞䗉࠰ളᇬᡌ൦॰ᐨ㔅ݷ䇮֒Ѱᓊ⭞䙊ᒬᣋ 271 12 ᭴ᐸ൰Ⲻ䇷᱄ᮽԬ 13 䗉࠰ളᇬᡌ൦॰㔅䗽〇ᆜ䈋僂䇷᱄ሯӰ㊱Ƚࣞὃ⢟ૂ⭕ᘷ 290 ⧥ູᰖᇩⲺ䍺ᯏ ⌞φ ѰҼф↙ᮽ॰࠼θᵢ䱺Ԭ䜞࠼ഴ㺞䠃᯦㕌ਭѰ䱺ഴ 1Ƚ䱺ഴ 2 ᡌ䱺㺞 1Ƚ 䱺㺞 2 ㅿȾ 141 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 1. ⴤⲺะഖⲺṮ㤭䞮ᓅࡍެ᧞ሲⲺ≞ะ䞮ᓅࡍ MON 87427 ⲴᨂޕᒿࡇབྷሿѪ 3746 bpˈ㘼 T-DNA ᨂޕᒿࡇབྷሿѪ 3681 bpDŽ 䲔 T-DNA ཆˈ൘T-DNAⲴ5'ㄟ઼ 3'ㄟ࠶࡛ᴹ 41 bp઼ 24 bp Ⲵᨂޕˈ䘉ᱟ൘ T-DNA ᨂޕ䗷〻ѝ㠚㹼ᨂޕⲴᒿࡇDŽT-DNA ѝⲴⴞⲴสഐ cp4 epsps ⲴབྷሿѪ 1368 bpDŽ cp4 epsps ⲴṨ㤧䞨ᒿࡇ㿱䱴മ 1ˈᖃҾ䱴മ 5˄MON 87427 ޘ䮯ᨂޕᒿࡇ઼ส 㘬 DNA ᒿࡇ˅ᡰ⽪Ⲵ 2995~4362 bp Ⲵս㖞DŽחഐ㓴 MON 87427 㺘䗮Ⲵ CP4 EPSPS 㳻ⲭབྷሿ㓖Ѫ 47.6 kDˈਜ਼ᴹ 455 њ≘ส䞨ˈ ṩᦞⴞⲴสഐṨ㤧䞨ᒿࡇ᧘ሬⲴ CP4 EPSPS 㳻ⲭⲴ≘ส䞨ᒿࡇ㿱䱴മ 2DŽ ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ 䱴മ1. MON 87427ѝcp4 epspsสഐⲴṨ㤧䞨ᒿࡇ˄CBI˅ 1 MLHGASSRPA TARKSSGLSG TVRIPGDKSI SHRSFMFGGL ASGETRITGL 51 LEGEDVINTG KAMQAMGARI RKEGDTWIID GVGNGGLLAP EAPLDFGNAA 101 TGCRLTMGLV GVYDFDSTFI GDASLTKRPM GRVLNPLREM GVQVKSEDGD 151 RLPVTLRGPK TPTPITYRVP MASAQVKSAV LLAGLNTPGI TTVIEPIMTR 201 DHTEKMLQGF GANLTVETDA DGVRTIRLEG RGKLTGQVID VPGDPSSTAF 251 PLVAALLVPG SDVTILNVLM NPTRTGLILT LQEMGADIEV INPRLAGGED 301 VADLRVRSST LKGVTVPEDR APSMIDEYPI LAVAAAFAEG ATVMNGLEEL 351 RVKESDRLSA VANGLKLNGV DCDEGETSLV VRGRPDGKGL GNASGAAVAT 401 HLDHRIAMSF LVMGLVSENP VTVDDATMIA TSFPEFMDLM AGLGAKIELS 451 DTKAA 䱴മ2. ṩᦞMON 87427ѝcp4 epspsสഐⲴṨ㤧䞨ᒿࡇ᧘ሬⲴ CP4 EPSPS㳻ⲭⲴ≘ส䞨ᒿࡇ 142 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߙԅୖٲԅݮ࿙ူၻં .2 MON 87427 Ⲵ䖜ॆ䖭փѪ PV-ZMAP1043 䍘㋂DŽPV-ZMAP1043 䍘㋂Ⲵབྷሿ 㓖Ѫ 8.9 kbˈवਜ਼࠶࡛⭡ᐖǃਣ䗩⭼࠶ᔰⲴ T-DNADŽT-DNA वਜ਼ањᆼᮤⲴ cp4 epsps สഐ㺘䗮ⴂˈ䈕สഐ㺘䗮ⴂवᤜ e35S ࣘᆀǃhsp70 Ⲵਜ਼ᆀǃCTP2 ਦ㔯փ䖜〫㛭ᒿࡇǃcp4 epsps 㕆⸱ᒿࡇ઼ nos 㓸→ᆀDŽPV-ZMAP1043 䍘㋂ᡰव 䜘䚇ՐݳԦ≷ᙫҾ䱴㺘 1ˈަ䍘㋂മ䉡㿱䱴മ 3DŽޘਜ਼Ⲵ PV-ZMAP1043 䍘㋂ᱟӪᐕᶴᔪਸᡀⲴˈަ僘ᷦᶕⓀҾབྷ㛐ᵶ㧼DŽབྷ㛐ᵶ㧼 ൘⧟ຳ䟼ᰐᡰн൘ˈҏᆈ൘ҾӪ㊫ㅹ㜺Ἶࣘ⢙Ⲵ⎸ॆ㌫㔏ѝ˄Jefferson et al., 1986˅DŽ⭡Ҿབྷ㛐ᵶ㧼Ⲵ䘉⭏⢙ᆖ⢩ᙗˈа㡜䇔Ѫ⋑ᴹᗵ㾱ਖཆ䘋㹼仍ཆᆹޘ ᙗ࠶᷀ᡆᇎ傼DŽ䈕㺘䗮䖭փнާᴹ仍ཆⲴ㠤⯵ᙗˈҏнਟ㜭╄ਈᡀѪާᴹ㠤⯵ᙗ Ⲵ䖭փˈሩӪ㊫઼ࣘ⢙ᱟᆹޘⲴDŽ PV-ZMAP1043 䍘㋂кⲴᐖ䗩⭼ᒿࡇ઼ਣ䗩⭼ᒿࡇᶕⓀҾṩⱼߌᵶ㧼 ˄Agrobacterium tumefaciens˅˄Barker et al., 1983; Depicker et al., 1982; Zambryski et al., 1982˅DŽ⇿њ䗩⭼ᒿࡇवਜ਼ᴹ 24-25 bp ൘䖜ॆᰦਟԕਁ⭏ DNA ӔᦒⲴ 㕪ս⛩DŽᐖਣ䗩⭼ᒿࡇሶ T-DNA ઼䍘㋂僘ᷦᒿࡇ࠶ᔰDŽ൘ PV-ZMAP1043 䍘 ㋂Ⲵ僘ᷦᒿࡇ४ฏवᤜᴹ㓶㧼༽ࡦ䎧⛩˄ori V ઼ ori-pBR322˅ǃ㓶㧼䘹ᤙḷ ᤱ൘བྷ㛐ᵶ㧼ѝⲴᤧ䍍ᮠⲴᕅᆀ㳻ⲭ䱫䙿⢙Ⲵ㕆⸱४˄rop˅˄䱴؍઼ 䇠สഐ aadA 㺘 1˅DŽн䇪ᱟ䘉ӋսҾ僘ᷦᒿࡇк䚇ՐݳԦ䘈ᱟ僘ᷦкⲴަᆳᒿࡇ൷⋑ᴹᮤਸ 䘋⦹㊣สഐ㓴˄䱴മ 10˅DŽ 143 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 B-Left Border Region OR-ori V P-e35S I-hsp70 Nsi I1719 Nco I1952 TS-CTP2 CS-rop T-DNA PV-ZMAP1043 Sph I2184 6 8946 bp OR-ori-pBR322 CS-cp4 epsps Nsi I3676 aadA T-nos Sph I3986 B-Right Border Region 䱴മ3. PV-ZMAP1043䍘㋂മ˄⽪ᵲӔ᧒䪸˅ кമѪُࣙҾߌᵶ㧼䖜ॆᯩ⌅ᗇࡠᣇ㥹⭈㟖䖜สഐ⦹㊣ MON 87427 ᰦᡰ˖⌘ ḷ᰾Ҷ䚇ՐݳԦ઼ח⭘㺘䗮䖭փ PV-ZMAP1043 Ⲵ⧟ᖒ䍘㋂മ䉡DŽ䍘㋂മཆ Southern ᵲӔ࠶᷀ᡰ⭘䲀ࡦᙗ࠷䞦ս⛩˄㋇փ˅ˈSouthern ᵲӔ࠶᷀ᡰ⭘᧒䪸˄᧒ DŽח䪸 1~7˅ሩս㖞ḷҾ⧟ᖒമ 144 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴㺘1. PV-ZMAP1043䍘㋂ᡰਜ਼䚇ՐݳԦᾲ䘠 䍘 ㋂ ѝ Ⲵ ˅⥞䚇ՐݳԦ ࣏㜭᧿䘠˄৲㘳᮷ ս㖞˄bp˅ T-DNA ṩⱼߌᵶ㧼 T-DNA ᐖ䗩⭼ᒿࡇˈ৲о T-DNA 䖜઼〫ᮤਸ B1-ᐖ䗩⭼ 1-442 ˄Barker et al., 1983˅ 䰤ᨂᒿࡇ 443-483 DNA ݻ䲶઼ᶴᔪ䴰㾱 P2-e35S वਜ਼ᴹ䟽༽໎ᕪ४ฏ˄Kay et al., 1987˅Ⲵ㣡ὠ㨌㣡ਦ⯵∂ 484-1104 ˄CaMV˅35S RNA Ⲵࣘᆀ˄Odelletal.,1985˅ 䰤ᨂᒿࡇ 1105-1125 DNA ݻ䲶઼ᶴᔪ䴰㾱 ⦹㊣✝◰㳻ⲭ 70 สഐⲴㅜањਜ਼ᆀ˄Brown and Santino, I3-hsp70 1126-1929 1997˅ 䰤ᨂᒿࡇ 1930-1953 DNA ݻ䲶઼ᶴᔪ䴰㾱 㕆⸱ᤏই㣕 EPSPS 䞦ਦ㔯փ䖜〫㛭४ฏⲴสഐ ShkG Ⲵ䶦 TS4-CTP2 1954-2181 ḷᒿࡇ˄Herrmann, 1995; Klee et al., 1987˅ˈᕅሬ CP4 EPSPS 䖜〫ࡠਦ㔯փ 㓿ᇶ⸱ᆀՈॆⲴṩⱼߌᵶ㧼 CP4 㧼Ṛ aroA สഐ㕆⸱ᒿࡇˈ CS5- 2182-3549 㕆⸱ CP4 EPSPS 㳻ⲭ˄Barry et al., 2001; Padgette et al., cp4 epsps 1996˅ 䰤ᨂᒿࡇ 3550-3555 DNA ݻ䲶઼ᶴᔪ䴰㾱 ṩⱼߌᵶ㧼㜝㜲⻡ਸ䞦สഐ˄nos˅3'ㄟ䶎䖜ᖅ४ˈ㓸→䖜 T6-nos 3556-3808 ᖅᒦфᕅሬ mRNA Ⲵཊ㚊㞪㤧䞨ॆ˄Bevan et al., 1983˅ 䰤ᨂᒿࡇ 3809-3835 DNA ݻ䲶઼ᶴᔪ䴰㾱 ṩⱼߌᵶ㧼 T-DNA ᐖ䗩⭼ᒿࡇˈ৲о T-DNA 䖜઼〫ᮤਸ B-ਣ䗩⭼ 3836-4192 ˄Depicker et al., 1982; Zambryski et al., 1982˅ 䍘㋂僘ᷦ DNA 䰤ᨂᒿࡇ 4193-4328 DNA ݻ䲶઼ᶴᔪ䴰㾱 䖜ᓗᆀ Tn7 Ⲵ≘ส㤧㊫ᣇ⭏㍐䫍ॆ䞦˄3''(9)-O-Ṩ㤧䞨䖜〫 aadA 4329-5217 䞦˅Ⲵࣘᆀǃ㕆⸱ᒿࡇ઼ ㄟ䶎䖜ᖅ४˄Fling et al., 1985˅ˈ ਟԕ֯㓶㧼ᣇᇴ᭮㓯㧼㍐઼䬮䴹㍐ 䰤ᨂᒿࡇ 5218-5747 DNA ݻ䲶઼ᶴᔪ䴰㾱 OR7-ori- pBR322 䍘㋂Ⲵ DNA ༽ࡦ䎧⛩ˈ㔤ᤱབྷ㛐ᵶ㧼˄E. coli˅ 5748-6336 pBR322 ѝ䍘㋂Ⲵ༽ࡦ˄Sutcliffe, 1979˅ 䰤ᨂᒿࡇ 6337-6763 DNA ݻ䲶઼ᶴᔪ䴰㾱 ColE1 䍘㋂ѝᕅᆀ㳻ⲭ䱫䙿⢙Ⲵ㕆⸱ᒿࡇˈ㔤ᤱབྷ㛐ᵶ㧼 CS-rop 6764-6955 ˄E. coli˅ѝ䍘㋂Ⲵᤧ䍍ᮠ˄Giza and Huang, 1989˅ 䰤ᨂᒿࡇ 6956-8463 DNA ݻ䲶઼ᶴᔪ䴰㾱 ᒯᇯѫ㤳ത䍘㋂ RK2 Ⲵ༽ࡦ䎧ս⛩ˈ㔤ᤱߌᵶ㧼 OR-ori V 8464-8860 ˄Agrobacterium˅ѝ䍘㋂༽ࡦ˄Stalker et al., 1981˅ 䰤ᨂᒿࡇ 8861-8946 DNA ݻ䲶઼ᶴᔪ䴰㾱 1 B–䗩⭼ᒿࡇ˗2 P–ࣘᆀ˗3 I–ਜ਼ᆀ˗4 TS –䖜〫㳻ⲭᒿࡇ˗5 CS –㕆⸱ᒿࡇ˗6 T– 㓸→ᆀ˗7 OR –༽ࡦ⛩ 145 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3. ⴤⲺะഖфὃ⢟ะഖ㓺᮪ਾެ㺞䗴Ⲻ࠼ᆆỶ⎁ᡌ䢪ᇐ㔉᷒ δPCR Ỷ⎁ȽSouthern ᵸӚ࠼᷆ȽNorthern ᡌ Western ࠼᷆㔉 ᷒ȽⴤⲺะഖӝ⢟㺞䗴㔉᷒ε ˅㘬ᒿࡇⲴ PCR ࠶᷀㔃᷌˄CBIחᒿࡇ৺ަ䛫Ⲵสഐ㓴ޕᨂ 3-1 ˅㘬ᒿࡇ˄CBIחᒿࡇ৺สഐ㓴ޕᨂ 3-2 㘬⡷⇥઼䞦࠷ս⛩㓯ᙗ⽪മחǃสഐ㓴⇥⡷ޕᨂ 3-3 3-4 ᨂޕᮠ઼ᤧ䍍ᮠⲴ Southern ᵲӔ࠶᷀㔃᷌ 3-5 MON 87427 ѝнਜ਼䍘㋂僘ᷦᒿࡇⲴ Southern ᵲӔ傼䇱 3-6 ᨂޕᒿࡇцԓっᇊᙗⲴ Southern ᵲӔ࠶᷀ 3-7 ᨂޕ⡷⇥㺘䗮ӗ⢙Ⲵ㺘䗮䟿࠶᷀ 3-8 ᨂޕ⡷⇥㺘䗮ӗ⢙൘ཊњцԓѝⲴ㺘䗮っᇊᙗ࠶᷀ 146 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅㘬ᒿࡇⲴ PCR ࠶᷀㔃᷌˄CBIחᒿࡇ৺ަ䛫Ⲵสഐ㓴ޕᨂ 3-1 ࡙⭘ PCR ᯩ⌅ᢙ໎Ҷ 3 њӂ䟽ਐⲴ DNA ⡷⇥˄䱴മ 4ˈӗ⢙ Aǃӗ⢙ B 㘬สഐ㓴ᒿࡇDŽPCR ᢙ໎ᡰחᒿࡇ৺ަޕ䮯ᨂޘӗ⢙ C˅ˈ䘉 3 њ⡷⇥⏥ⴆҶ઼ ⭘ᕅ⢙઼ᶑԦ㿱䱴㺘 2DŽሶ䘉 3 њᢙ໎ӗ⢙䘋㹼ᒿࡇ࠶᷀ᒦ᧕ˈণਟ⺞ᇊޘ䮯 ޕ㘬ᒿࡇ˄䱴മ 5˅DŽṩᦞᒿࡇ࠶᷀㔃᷌ˈMON 87427 Ⲵᨂחᒿࡇ৺สഐ㓴ޕᨂ ᒿࡇབྷሿѪ 3746 bpˈަѝ T-DNA ᨂޕѪ 3681 bpDŽਖཆ൘ T-DNA Ⲵ 5'ㄟ઼ 3' ㄟ࠶࡛ᴹ 41 bp ઼ 24 bp Ⲵᨂޕˈ䘉ᱟ൘ T-DNA ᨂޕ䗷〻ѝ㠚㹼ᨂޕⲴᒿࡇDŽ䘉 䗷〻ѝᑨ㿱ˈਟ㜭ᱟ⭡Ҿৼ䬮ᯝ㻲؞༽ᵪࡦᕅ䎧ޕ⧠䊑൘ཆⓀ DNA ᨂ ˄Salomon and Puchta, 1998˅DŽ ↔ཆˈᒿࡇ࠶᷀ҏ䇱ᇎ T-DNA cp4 epsps สഐ㺘䗮ⴂᱟᆼᮤⲴˈަѝ䚇 ՐݳԦⲴᧂࡇᯩᔿоަ൘ PV-ZMAP1043 䖜ॆ䍘㋂кⲴᧂࡇᯩᔿа㠤DŽ ᒿࡇޕ㘬ᒿࡇԕ৺㔃ᶴ㿱䱴മ 5 ઼䱴മ 6˗ᨂחᒿࡇ઼สഐ㓴ޕMON 87427 Ⲵᨂ ѝⲴ䈳᧗ݳԦ᧿䘠㿱䱴㺘 3DŽ 147 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᒿࡇPCRᢙ໎࠶᷀˄CBIחᒿࡇ৺สഐ㓴ᯱޕ䮯ᨂޘ䱴㺘2. MON 87427 ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ 148 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᒿࡇѝⲴ䚇Ր䈳᧗ݳԦ৺ަᆳᒿࡇ≷ᙫޕ䱴㺘3. MON 87427ᨂ ˅⥞䚇ՐݳԦ བྷሿ˄ս㖞*˅ ࣏㜭᧿䘠˄৲㘳᮷ 1003 bp ս⛩ 5'ㄟ䛫Ⲵ⦹㊣สഐ㓴 DNAޕ㘬ᒿࡇ ᨂחㄟ'5 ˄1-1003˅ 252 bp ṩⱼߌᵶ㧼 T-DNA ᐖ䗩⭼ᒿࡇˈ৲о T-DNA T-DNA ᐖ䗩⭼ᒿࡇ ˄1004-1255˅ 䖜઼〫ᮤਸ˄Barker et al., 1983˅ 41 bp 䰤ᨂᒿࡇ DNA ݻ䲶઼䖭փᶴᔪ䴰㾱 ˄1256-1296˅ वਜ਼ᴹ䟽༽໎ᕪ४ฏ˄Kay et al., 1987˅Ⲵ㣡 621 bp e35S ࣘᆀ ὠ㨌㣡ਦ⯵∂˄CaMV˅35S RNA Ⲵࣘᆀ ˄1297-1917˅ ˄Odell et al., 1985˅ 21 bp 䰤ᨂᒿࡇ DNA ݻ䲶઼ᶴᔪ䴰㾱 ˄1918-1938˅ 804 bp ⦹㊣✝◰㳻ⲭ 70 สഐⲴㅜањਜ਼ᆀ˄Brown hsp70 ਜ਼ᆀ ˄1939-2742˅ and Santino, 1997˅ 24 bp 䰤ᨂᒿࡇ DNA ݻ䲶઼䖭փᶴᔪ䴰㾱 ˄2743-2766˅ 㕆⸱ᤏই㣕 EPSPS 䞦ਦ㔯փ䖜〫㛭४ฏⲴส 228 bp CTP2 ਦ㔯փ䖜〫㛭 ഐ ShkG Ⲵ䶦ḷᒿࡇ˄Herrmann, 1995; Klee et ˄2767-2994˅ al., 1987˅ˈᕅሬ CP4 EPSPS 䖜〫ࡠਦ㔯փ 㓿ᇶ⸱ᆀՈॆⲴṩⱼߌᵶ㧼 CP4 㧼Ṛ aroA ส 1368 bp cp4 epsps 㕆⸱ᒿࡇ ഐ㕆⸱ᒿࡇˈ㕆⸱ CP4 EPSPS 㳻ⲭ˄Barry et ˄2995-4362˅ al., 1992; Padgette et al., 1996˅ 6bp 䰤ᨂᒿࡇ DNA ݻ䲶઼䖭փᶴᔪ䴰㾱 ˄4363-4368˅ ṩⱼߌᵶ㧼㜝㜲⻡ਸ䞦สഐ˄nos˅3'ㄟ䶎䖜ᖅ 253 bp nos 㓸→ᆀ ४ˈ㓸→䖜ᖅᒦфᕅሬ mRNA Ⲵཊ㚊㞪㤧䞨 ˄4369-4621˅ ॆ˄Bevan et al., 1983˅ 27 bp 䰤ᨂᒿࡇ DNA ݻ䲶઼䖭փᶴᔪ䴰㾱 ˄4622-4648˅ ṩⱼߌᵶ㧼 T-DNA ਣ䗩⭼ᒿࡇˈ৲о T-DNA 36 bp T-DNA ਣ䗩⭼ᒿࡇ 䖜઼〫ᮤਸ˄Depicker et al., 1982; Zambryski ˄4649-4684˅ et al., 1982˅ 1092 bp ս⛩ 3'ㄟ䛫Ⲵ⦹㊣สഐ㓴 DNAޕ㘬ᒿࡇ ᨂחㄟ'3 ˄4685-5776˅ 㘬ᒿࡇ˄㿱䱴മ 5˅Ⲵᓄս㖞DŽחᒿࡇ઼ޕᤜਧḷࠪⲴս㖞ሩᓄҾ MON 87427 ᨂ * 149 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ ˅㘬สഐ㓴⡷⇥Ⲵ䟽ਐPCR࠶᷀˄CBIח४৺ޕ䱴മ4. ⏥ⴆMON 87427ᨂ ᢙ໎⡷⇥Ⲵ亴ᵏབྷሿᱮ⽪൘ PCR ӗ⢙ࠍ㜦മлᯩⲴ MON 87427 ᨂޕᒿࡇ㓯 ᖒമкDŽ⇿⌣䚃кṧ䟿Ѫ 4~9 lPCRᢙ໎ӗ⢙DŽ⌣䚃䈤᰾ྲл˖ ⌣䚃 ṧ૱᧿䘠 1 1 kb DNA ࠶ᆀ䟿ḷ䇠 2 MON 87427 3 ሩ➗ LH198 × HiII 4 ᰐ DNA ⁑ᶯሩ➗ 5 MON 87427 6 ሩ➗ LH198 × HiII 7 ᰐ DNA ⁑ᶯሩ➗ 8 MON 87427 9 ሩ➗ LH198 × HiII 10 ᰐ DNA ⁑ᶯሩ➗ 11 1 kb DNA Ladder ㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠 150 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅㘬ᒿࡇ˄CBIחᒿࡇ৺สഐ㓴ޕᨂ 3-2 ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ ˅㘬⦹㊣สഐ㓴ᒿࡇ˄CBIחᒿࡇ઼ޕ䮯ᨂޘ 䱴മ 5. MON 87427 151 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㘬⡷⇥઼䞦࠷ս⛩㓯ᙗ⽪മחǃสഐ㓴⇥⡷ޕᨂ 3-3 䱴㺘4. MON 87427ⲴSouthernᵲӔѝ䲀ࡦᙗ࠷䞦ǃᵲӔ᧒䪸઼亴ᵏ⡷⇥≷ᙫ Southern ᵲӔ㔃᷌ 䱴മ 7 䱴മ 8 䱴മ 9 䱴മ 10 ⭘Ⲵ᧒䪸 ᧒䪸 1 ઼ 4 ᧒䪸 2 ᧒䪸 3 ᧒䪸 5ǃ6 ઼ 7֯ Ự⍻ⴞⲴ ᨂޕ/ᤧ䍍ᮠ ᨂޕ/ᤧ䍍ᮠ ᨂޕ/ᤧ䍍ᮠ 䍘㋂僘ᷦᒿࡇ ᧒䪸䶦ḷᒿࡇ ࠷䞦 亴ᵏ Southern ᵲӔؑਧ ~7.1 kb ~7.1 kb 䍘㋂ PV-ZMAP1043 Sph I ~7.1 kb ~7.1 kb ~1.8 kb ~1.8 kb ~1.8 kb ~1.2 kb ᧒䪸⁑ᶯ --* ~1.0 kb ~1.5 kb ~1.5 kb ~0.7 kb ~1.7 kb ı 2.8 kb ı 2.8 kb Nco I ~2.2 kb ᰐᵲӔᶑᑖ ~2.2 kb ~2.2 kb MON 87427 ~1.7 kb ~2.0 kb Nsi I ~2.0 kb ~2.0 kb ᰐᵲӔᶑᑖ ~1.7 kb ı 1.3 kb *㺘⽪ᵚ⭘ᓄ䞦䘋㹼䞦࠷࠶᷀ 152 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Nsi I 2533 Nsi I 4490 Nco I4931 Nsi I 795 Nco I2766 r1 r1 1 nos - e35S - T hsp70 CTP2 5776 - - P I TS cp4 epsps - CS Left Border Region - B B-Right Border Region Probe 1 Probe 2 Probe 3 Probe 4 ~ 2.2 kb Nco I ~ 1.7 kb ~ 2.0 kb Nsi I 㘬ᒿࡇ৺亴ᵏ䞦࠷⡷⇥བྷሿⲴ㓯ᙗ⽪മחᒿࡇǃสഐ㓴ޕ䱴മ6. MON 87427ᨂ ᒿࡇлᯩ㇝ཤ㺘⽪䖜ᖅᯩੁˈⴤ䀂㇝ཤ㺘᰾ޕᒿࡇ䚇Ր㓴ᡀݳԦ઼ Southern ᵲӔ࠶᷀ѝⲴ䲀ࡦᙗ䞦࠷ս⛩ˈᨂޕമк䜘㺘⽪ᨂ 㘬ᒿࡇⲴᔰս㖞઼ᯩੁDŽമл䜘ᡰ⽪Ѫ䲀ࡦᙗ䞦Ⲵ亴ᵏ䞦࠷⡷⇥བྷሿˈᵜ⽪മᒦᵚ᤹∄ֻ㔈ࡦˈഐ↔䚇ՐݳԦⲴח㊣สഐ㓴⦹ ս㖞઼ T-DNA ᧒䪸བྷሿѪ䘁լ٬DŽ 153 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3-4 ᨂޕᮠ઼ᤧ䍍ᮠⲴ Southern ᵲӔ࠶᷀㔃᷌ ⭘䲀ࡦᙗ࠷䞦 Nco I ᡆ Nsi I ⎸ॆ MON 87427 สഐ㓴 DNAˈԕ㜭ཏ⏥ⴆ T-DNA ᒿࡇޘ䮯Ⲵӂ䟽ਐ᧒䪸˄᧒䪸 1~᧒䪸 4ˈ䱴മ ઼䱴മ 6˅䘋㹼 Southern ᵲӔ࠶᷀ˈӾ㘼⺞ᇊ MON 87427 ѝⲴᨂޕս⛩ᮠ઼ᨂޕᤧ䍍ᮠDŽ 㘬ᒿࡇⲴ㓯ᙗ⢙⨶മ䉡˄䱴മ 6˅ˈ⭘䲀ࡦᙗ࠷䞦חᒿࡇ৺ަޕṩᦞᨂ 㘬ᒿࡇкᴹ 1חⲴ T-DNA 䜘ਚᴹ 1 њ䞦࠷ս⛩ˈᒦ൘䛫Ⲵ 3'ㄟޕNco I ൘ᨂ њ䞦࠷ս⛩DŽྲ᷌ T-DNA ൘ MON 87427 ѝѪঅս⛩অᤧ䍍ᨂޕˈࡉ亴ᵏ⭘ Nco I 䞦⎸ॆ MON 87427 สഐ㓴 DNA ਾ㜭ཏӗ⭏ 2 њ䞦࠷⡷⇥ˈަѝањ⡷⇥བྷሿ ˈ㘬ᒿࡇח亴ᵏѪ~2.2 kbˈवਜ਼ CTP2ǃcp4 epspsǃnosǃਣ䗩⭼ᒿࡇ઼ 3'ㄟ䜘࠶ 㘬ᒿࡇǃᐖ䗩⭼ᒿࡇǃח㘼ਖཆањ䗩⭼⡷⇥亴ᵏı2.8 kbˈवਜ਼ᵚ⸕བྷሿⲴ 5'ㄟ e35S ઼ hsp70DŽ 㘬ᒿח䲀ࡦᙗ࠷䞦 Nsi I ൘ T-DNA 䜘ᴹ 2 њ䞦࠷ս⛩ˈ൘ަ䛫᧕Ⲵ 5'ㄟ ࡇкᴹ 1 њ䞦࠷ս⛩DŽྲ᷌ T-DNA ൘ MON 87427 ѝѪঅս⛩অᤧ䍍ᨂޕˈࡉ 亴ᵏ⭘ Nsi I ⎸ॆ MON 87427 สഐ㓴 DNA ਾ㜭ཏӗ⭏ 3 њ䞦࠷⡷⇥DŽަѝаᶑ ˗㘬ᒿࡇǃᐖ䗩⭼ᒿࡇǃe35S ઼䜘࠶ hsp70חkbˈ䈕ᶑᑖवᤜᐢ⸕བྷሿⲴ 5'ㄟ 1.7~ аᶑᑖ~2.0 kbˈवᤜ䜘࠶ hsp70ǃCTP2ǃcp4 epsps ઼䜘࠶ nos˗ਖཆаᶑᑖı1.3 kbˈ 㘬ᒿࡇDŽחवᤜ䜘࠶ nosǃਣ䗩⭼ᒿࡇ઼ᵚ⸕བྷሿⲴ 3'ㄟ MON 87427 ᱟԕ LH198 × HiII ᵲӔѪਇփ䖜ॆⲴˈഐ↔䟷⭘ LH198 × HiII Ѫ䱤ᙗሩ➗ˈ਼ᰦ⭘㓿 Sph I 䞦࠷Ⲵ PV-ZMAP1043 䍘㋂ DNA ᡆ ⺞᧒䪸Ѫ䱣ᙗሩ➗DŽ䱣ᙗሩ➗кṧ䟿Ѫ~0.1 ؽ઼~1.0 ؽสഐ㓴 DNA Ⲵ䟿ˈԕ ᇊ Southern ᵲӔⲴ⚥ᓖDŽ⭘᧒䪸 1~4˄䱴മ ઼䱴മ 6˅䘋㹼 Southern ᵲӔਟ 㜭Պࠪ⧠ⲴᵲӔᑖර≷ᙫҾ䱴㺘 4ˈᵲӔ㔃᷌㿱䱴മ 7ǃമ 15 ઼䱴മ 9DŽ 1˅᧒䪸 1 ઼᧒䪸 4 ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆᰦˈ൘~6.1 kb ઼~4.1 kb ༴ӗ⭏ⓀᙗᵲӔᶑᑖ˄䱴മ 7ˈ⌣䚃 1 ઼ 8˅˗㘼⭘ Nsi I ⎸ॆᰦˈ൘~9.8 kb ⍻kb ༴ӗ⭏ⓀᙗᵲӔᶑᑖ˄䱴മ 7ˈ⌣䚃 3 ઼ 10˅DŽ䘉ӋᵲӔؑਧ൘ 4.3~઼ ⢩䈅⢙઼ᑨ㿴ሩ➗⌣䚃ѝ൷ᆈ൘ˈഐ↔䇔Ѫ䘉ӋؑਧᱟⓀᙗⲴ㛼ᲟᵲӔˈᒦ䶎 DNADŽ䘉Ӌؑਧᖸਟ㜭ᱟ⭡᧒䪸 1оⓀ Hsp70 ޕᔲᙗൠ䪸ሩMON 87427ѝⲴᨂ ᒿࡇⲴᵲӔᕅ䎧ⲴˈഐѪ䈕᧒䪸ਜ਼ᴹаሿ䜘࠶ Hsp70 ਜ਼ᆀ˄䱴മ 6˅DŽ⭡Ҿ᧒ 䪸 1 ӵ㾶ⴆҶ Hsp70 ਜ਼ᆀѝᖸሿа䜘࠶ᒿࡇˈᵲӔؑਧሩ䖳ᕡDŽ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо亴ݸ⭘ Sph I ⎸ॆⲴ PV-ZMAP1043 䍘㋂ DNA ਸ˄䱴മ 7ˈ⌣䚃 7˅ˈ䲔к䘠ⓀᙗᵲӔᶑᑖཆˈ 䘈൘~7.1 kb ઼~1.8 kb ༴ӗ⭏ 2 ᶑ亴ᵏᶑᑖ˄䱴മ 6ǃ䱴㺘 4˅DŽ䱣ᙗሩ➗ᵲӔؑ ਧⲴỰࠪˈ㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔDŽ MON 87427 สഐ㓴 DNA ԕ Nco I ⎸ॆ˄䱴മ 7ˈ⌣䚃 2 ઼ 9˅ˈ䲔к䘠 ⓀᙗᵲӔᶑᑖཆˈ䘈൘~5.5 kb ઼~2.2 kb ༴ӗ⭏ 2 ᶑᑖDŽަѝˈ~5.5 kb ᶑᑖԓ㺘 㘬สഐ㓴 DNAˈㅖਸı2.8 kb Ⲵ亴ᵏ䗩⭼⡷⇥བྷሿ˄䱴חT-DNA ৺ަ䛫᧕ ޕҶᨂ 㘬สחDNA Ⲵ 3'ᵛㄟ৺ަ 3'ㄟ䛫᧕Ⲵ ޕമ 6ǃ䱴㺘 4˅˗~2.2 kb ᶑᑖԓ㺘Ҷᨂ 䱴മ 6ǃ䱴㺘 4˅DŽ˄ഐ㓴 DNAˈ䈕㔃᷌ㅖਸ~2.2 kb 䗩⭼⡷⇥Ⲵ亴ᵏ٬ 154 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 MON 87427 สഐ㓴 DNA ԕ Nsi I ⎸ॆ˄䱴മ 7ˈ⌣䚃 4 ઼ 11˅ˈ䲔к䘠 ⓀᙗᵲӔᶑᑖཆˈ䘈൘~6.4 kbǃ~2.0 kb ઼~1.7 kb ༴ӗ⭏ 3 ᶑᑖDŽަѝˈ~1.7 kb 㘬สഐ㓴 DNAˈㅖ ਸ ~1.7 kbחⲴ T-DNA 5'ㄟ৺ަ䛫᧕Ⲵаሿ䜘࠶ޕԓ㺘ᨂ⇥⡷ DNA 䜘Ⲵа䜘 ޕ䱴മ 6ǃ䱴㺘 4˅˗~2.0 kb ᶑᑖԓ㺘ᨂ˄Ⲵ䗩⭼⡷⇥亴ᵏ٬ ࠶ᒿࡇˈㅖਸ~2.0 kb Ⲵ亴ᵏ⡷⇥བྷሿ˄䱴മ 6ǃ䱴㺘 4˅˗~6.4 kb ᶑᑖԓ㺘ᨂޕ 㘬สഐ㓴 DNAˈㅖਸı1.3 kb 䗩⭼⡷⇥Ⲵ亴ᵏབྷח'T-DNA Ⲵ 3'ㄟ৺ަ䛫᧕Ⲵ 3 ሿ˄䱴മ 6ǃ䱴㺘 4˅DŽ 䱴മ 7 ᡰ⽪㔃᷌㺘᰾ˈ᧒䪸 1 ઼᧒䪸 4 ᡰ⏥ⴆⲴᒿࡇӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ MON 87427 ⲴঅњਟỰ⍻สഐᓗѝDŽ 2˅᧒䪸 2 ᑨ㿴ሩ➗ LH198 × HiII ઼ MON 87427 Ⲵสഐ㓴 DNA ࠶࡛⭘ Nco I ᡆ Nsi I ⢙മ 15˅ˈ൷ӗ⭏ҶаӋⓀᙗᵲӔؑਧˈ䘉ӋᵲӔؑਧ൷ᆈ൘Ҿ⍻䈅˄ॆ⎸ ᑨ㿴ሩ➗⌣䚃ѝ˄മ 15ˈ⌣䚃 1~11˅DŽഐ↔䇔Ѫ䘉ӋؑਧᱟⓀᙗⲴ㛼Ჟᵲ઼ DNADŽ䘉Ӌؑਧᖸਟ㜭ᱟ⭡᧒䪸 2 ޕӔˈᒦ䶎⢩ᔲᙗൠ䪸ሩ MON 87427 ѝⲴᨂ оⓀ hsp70 ᒿࡇⲴᵲӔᕅ䎧ⲴˈഐѪ䈕᧒䪸ਜ਼ᴹᖸབྷа䜘࠶ hsp70 ਜ਼ᆀᒿࡇ ˄䱴മ ǃ䱴മ 6˅DŽ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆ˄മ 15ˈ⌣䚃 1 ઼ 8˅ˈ ൘~4.1 kb ༴ӗ⭏ⓀᙗᵲӔᶑᑖ˗ԕ Nsi I ⎸ॆ˄മ 15ˈ⌣䚃 3 ઼ 10˅ˈ൘~5.2 kb ઼~4.2 kb ༴ӗ⭏ⓀᙗᵲӔᶑᑖDŽ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘 ㋂ӗ⭏Ⲵ᧒䪸⁑ᶯਸ˄䱴മ ˈ᧒䪸 2˅ˈ䲔к䘠ⓀᙗᵲӔᶑᑖཆˈ䘈൘~1.0 kb ༴ӗ⭏亴ᵏᶑᑖ˄മ 15ˈ⌣䚃 5 ઼ 6˅DŽᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо亴ݸ⭘ Sph I ⎸ॆⲴ PV-ZMAP1043 䍘㋂ DNA ਸ˄മ 15ˈ⌣䚃 䲔к䘠ⓀᙗᵲӔᶑᑖཆˈ䘈൘~7.1 kb ༴ӗ⭏亴ᵏᶑᑖDŽ䱣ᙗሩ➗ᵲӔؑˈ˅7 ਧⲴỰࠪ㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔDŽ MON 87427 สഐ㓴 DNA ԕ Nco I ⎸ॆ˄മ 15ˈ⌣䚃 2 ઼ 9˅ˈ䲔к䘠Ⓚ ᙗᵲӔᶑᑖཆˈ䘈൘~5.5 kb ઼~2.2 kb ༴ӗ⭏ 2 ᶑᑖDŽަѝˈ~5.5 kb ᶑᑖԓ㺘Ҷ 㘬สഐ㓴 DNAˈㅖਸı2.8 kb Ⲵ亴ᵏ䗩⭼⡷⇥བྷሿ˄䱴חT-DNA ৺ަ䛫᧕ ޕᨂ 㘬สחDNA Ⲵ 3'ᵛㄟ৺ަ 3'ㄟ䛫᧕Ⲵ ޕമ 6ǃ䱴㺘 4˅˗~2.2 kb ᶑᑖԓ㺘Ҷᨂ 䱴മ 6ǃ䱴㺘 4˅DŽ˄ഐ㓴 DNAˈ䈕㔃᷌ㅖਸ~2.2 kb 䗩⭼⡷⇥Ⲵ亴ᵏ٬ MON 87427 สഐ㓴 DNA ԕ Nsi I ⎸ॆ˄മ 15ˈ⌣䚃 4 ઼ 11˅ˈ䲔к䘠Ⓚ ᙗᵲӔᶑᑖཆˈ䘈൘~2.0 kb ઼~1.7 kb ༴ӗ⭏ 2 ᶑᑖDŽަѝˈ~1.7 kb ⡷⇥ᨂޕ ⇥⡷㘬สഐ㓴 DNAˈㅖਸ~1.7 kb Ⲵ䗩⭼חT-DNA Ⲵ 5'ᵛㄟ઼аሿ䜘࠶䛫᧕Ⲵ- DNA Ⲵ䜘ᒿࡇˈㅖਸ~2.0 kb ޕ䱴മ 6ǃ䱴 㺘 4˅˗~2.0 kb ᶑᑖਜ਼ᴹᨂ˄亴ᵏ٬ Ⲵ亴ᵏ⡷⇥བྷሿ˄䱴മ 6ǃ䱴㺘 4˅DŽ മ 15 ᡰ⽪㔃᷌㺘᰾ˈ᧒䪸 2 ᡰ⏥ⴆⲴᒿࡇӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ MON 87427 ⲴঅњਟỰ⍻สഐᓗѝDŽ 3˅᧒䪸 3 155 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᑨ㿴ሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA ⭘ Nco ˄䱴മI 9ˈ⌣䚃 1 ઼ 8˅ᡆ Nsi I ˄䱴മ 9ˈ⌣䚃 3 ઼ 10˅࠶࡛⎸ॆˈྲ亴ᵏᡰᯉˈ䈕䱤ᙗሩ➗⋑ᴹӗ⭏ਟỰ⍻Ⲵ ᵲӔᶑᑖDŽ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘 ㋂ӗ⭏Ⲵ᧒䪸⁑ᶯਸ˄䱴മˈ᧒䪸 3˅ˈ൘ ~1.5 kb ༴ӗ⭏亴ᵏᶑᑖ˄䱴മ 9ˈ 䚃 5 ઼ 6˅DŽᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо亴ݸ⭘ Sph I⌣ ⎸ॆⲴ PV-ZMAP1043 䍘㋂ DNA ਸ˄䱴മ 9ˈ⌣䚃 7˅ˈ൘~1.8 kb ༴ӗ⭏亴 ᵏᶑᑖ˄䱴മ 6ǃ䱴㺘 4˅DŽᶕ㠚Ҿ䍘㋂Ⲵਖа Sph I ⡷⇥˄~7.1 kb˅ਜ਼ᴹ᧒䪸 3 ᒿࡇⲴаሿ䜘࠶ˈн䗷൘ᵜ⹄ウѝⲴᵲӔ৽ᓄᶑᑖлᵚ㜭ӗ⭏ਟỰ⍻ⲴᵲӔؑਧDŽ 䱣ᙗሩ➗ᵲӔؑਧⲴỰࠪ㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔDŽ MON 87427 สഐ㓴 DNA ԕ Nco I ⎸ॆ˄䱴മ 9ˈ⌣䚃 2 ઼ 9˅ˈ൘~2.2 kb 㘬สחDNA Ⲵ 3'ᵛㄟ৺ަ 3'ㄟ䛫᧕Ⲵ ޕ༴ӗ⭏ 1 ᶑᑖDŽ䈕~2.2 kb ᶑᑖԓ㺘Ҷᨂ 䱴മ 6ǃ䱴㺘 4˅DŽMON 87427˄ഐ㓴 DNA˗䈕㔃᷌ㅖਸ~2.2 kb 䗩⭼⡷⇥Ⲵ亴ᵏ٬ สഐ㓴 DNA ԕ Nsi I ⎸ॆ˄䱴മ 9ˈ⌣䚃 4 ઼ 11˅ˈ൘~2.0 kb ༴ӗ⭏ 1 ᶑᑖˈ ↓ྲ亴ᵏⲴаṧ˄䱴മ 6ǃ䱴 㺘 4˅DŽ䈕 ~2.0 kb ᶑᑖਜ਼ᴹᨂޕ DNA Ⲵ䜘ᒿࡇDŽ 䱴മ 9 ᡰ⽪㔃᷌㺘᰾ˈ᧒䪸 3 ᡰ⏥ⴆⲴᒿࡇӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ MON 87427 ⲴঅњਟỰ⍻สഐᓗѝDŽ 156 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴മ7. MON 87427ѝT-DNAᨂޕս⛩઼ᤧ䍍ᮠⲴSouthernঠ䘩˄᧒䪸1઼᧒䪸4˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢DŽ᧒䪸 1 ઼᧒䪸 4 ӵ㾶ⴆ䜘࠶ T-DNA˄䱴 മ઼മ13˅DŽ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ࠷䞦⎸ॆⲴ DNA ṧ૱DŽ⌣䚃䈤᰾ྲ л˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ [᧒䪸 1 ઼᧒䪸 4 [~0.1 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 5 [᧒䪸 1 ઼᧒䪸 4 [~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 6 [䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 7 8 ሩ➗ LH198 × HiII˄Nco I˅ 9 MON 87427˄Nco I˅ 10 ሩ➗ LH198 × HiII˄Nsi I˅ 11 MON 87427˄Nsi I˅ ㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠 157 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴മ8. MON 87427ѝT-DNAᨂޕս⛩઼ᤧ䍍ᮠⲴSouthernঠ䘩˄᧒䪸2˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢DŽ᧒䪸 2 ӵ㾶ⴆ䜘࠶ T-DNA˄മ 6 ઼മ 13˅DŽ ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ࠷䞦⎸ॆⲴ DNA ṧ૱DŽ⌣䚃䈤᰾ྲл˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ [᧒䪸 2 [~0.1 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 5 [᧒䪸 2 [~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 6 [䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 7 8 ሩ➗ LH198 × HiII˄Nco I˅ 9 MON 87427˄Nco I˅ 10 ሩ➗ LH198 × HiII˄Nsi I˅ 11 MON 87427˄Nsi I˅ ㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠 158 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴മ9. MON 87427ѝT-DNAᨂޕս⛩઼ᤧ䍍ᮠⲴSouthernঠ䘩˄᧒䪸3˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢DŽ᧒䪸 3 ӵ㾶ⴆ䜘࠶ T-DNA˄䱴മ ઼മ 13˅DŽ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ࠷䞦⎸ॆⲴ DNA ṧ૱DŽ⌣䚃䈤᰾ྲл˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ [᧒䪸 3 [~0.1 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 5 [᧒䪸 3 [~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 6 [䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅ 7 8 ሩ➗ LH198 × HiII˄Nco I˅ 9 MON 87427˄Nco I˅ 10 ሩ➗ LH198 × HiII˄Nsi I˅ 11 MON 87427˄Nsi I˅ ㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠 159 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3-5 MON 87427 ѝнਜ਼䍘㋂僘ᷦᒿࡇⲴ Southern ᵲӔ傼䇱 ൘ MON 87427 ᨂޕᒿࡇѝнवਜ਼ PV-ZMAP1043 䍘㋂Ⲵԫօ僘ᷦᒿࡇDŽ਼ ṧ⭘ Nco I ᡆ Nsi I 䲀ࡦᙗ࠷䞦⎸ॆ MON 87427 สഐ㓴 DNAˈᒦ⭘᧒䪸 5~7 䘋㹼ᵲӔਟԕ⺞ᇊ MON 87427 ᱟਜ਼ᴹ僘ᷦᒿࡇDŽ᧒䪸 5~7 ਟԕ㾶ⴆ PV-ZMAP1043 䍘㋂Ⲵޘ䜘僘ᷦᒿࡇ˄䱴മ ˅DŽ⭘᧒䪸 5~7 䘋㹼 Southern ᵲӔ ਟ㜭Պࠪ⧠ⲴᵲӔᑖර≷ᙫҾ䱴㺘 4DŽSouthern ᵲӔ㔃᷌㿱䱴മ 10DŽ ᑨ㿴ሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA ⭘ Nco I˄䱴മ 10ˈ⌣䚃 1 ઼ 10˅ᡆ Nsi I˄䱴മ 10ˈ⌣䚃 3 ઼ 12˅࠶࡛⎸ॆˈ⭘ਟԕ㾶ⴆ PV-ZMAP1043 䍘㋂僘ᷦ ᒿࡇⲴ᧒䪸 5ǃ᧒䪸 6 ઼᧒䪸 7 ਼ᰦᵲӔˈ↓ྲ䱤ᙗሩ➗亴ᵏᓄᴹ㔃᷌аṧˈ⋑ ᴹӗ⭏ਟỰ⍻ⲴᵲӔᶑᑖDŽᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾ о㓿 Sph I ⎸ॆⲴ PV-ZMAP1043 䍘㋂ਸˈ൘~7.1 kb ༴ӗ⭏亴ᵏᶑᑖ˄䱴മ 10ˈ ⌣䚃 9˗䱴㺘 4˅DŽᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘㋂ӗ⭏Ⲵ᧒䪸 5 ઼᧒䪸 6˄䱴മ ˅Ⲵ⁑ᶯਸˈ൘~1.8 kb ઼ ~1.5 kb ༴ӗ⭏ 2 ᶑ亴ᵏᶑᑖ˄䱴മ 10ˈ⌣䚃 5 ઼ 6˗䱴㺘 4˅DŽᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘㋂ӗ⭏Ⲵ᧒䪸 7˄䱴മ ˅Ⲵ ⁑ᶯਸˈ൘~1.7 kb ༴ӗ⭏亴ᵏᶑᑖ˄䱴മ 10ˈ⌣䚃 7 ઼ 8˗䱴㺘 4˅DŽ䘉Ӌ䱣 ᙗሩ➗ᵲӔؑਧⲴỰࠪ㔃᷌㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔDŽ ᑨ㿴ሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA ⭘ Nco I˄䱴മ 10ˈ⌣䚃 2 ઼ 11˅ᡆ Nsi I˄䱴മ 10ˈ⌣䚃 4 ઼ 13˅࠶࡛⎸ॆˈ⭘ਟԕ㾶ⴆ PV-ZMAP1043 䍘㋂僘ᷦ ᒿࡇⲴ᧒䪸 5ǃ᧒䪸 6 ઼᧒䪸 7 ਼ᰦᵲӔˈ↓ྲ亴ᵏᓄᴹ㔃᷌аṧ˄䱴㺘 4˅ˈ ⋑ᴹԫօᵲӔᶑᑖࠪ⧠DŽ䘉а㔃᷌㺘᰾ˈMON 87427 нਜ਼ᴹԫօ PV-ZMAP1043 䍘㋂Ⲵ僘ᷦᒿࡇDŽ 160 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴മ10. MON 8742нਜ਼䍘㋂僘ᷦᒿࡇⲴSouthernᵲӔ࠶᷀˄᧒䪸5~7˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢DŽ᧒䪸 5ǃ᧒䪸 6 ઼᧒䪸 7 ਟԕ㾶ⴆޘ䜘 PV-ZMAP1043 䍘㋂Ⲵ僘ᷦᒿࡇ˄䱴മ ˅DŽ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ࠷䞦⎸ ॆⲴ DNA ṧ૱DŽ⌣䚃䈤᰾ྲл˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 5 ઼᧒䪸 6[~0.1ؽสഐ㓴ᖃ䟿]ਸ 5 ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 5 ઼᧒䪸 6[~1.0ؽสഐ㓴ᖃ䟿]ਸ 6 ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 7 [~0.1 ؽสഐ㓴ᖃ䟿]ਸ 7 ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 7 [~1.0 ؽสഐ㓴ᖃ䟿]ਸ 8 ሩ➗ LH198 × HiII˄Nco I˅䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿]ਸ 9 10 ሩ➗ LH198 × HiII˄Nco I˅ 11 MON 87427˄Nco I˅ 12 ሩ➗ LH198 × HiII˄Nsi I˅ 13 MON 87427˄Nsi I˅ ㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠 3-6 ᨂޕᒿࡇцԓっᇊᙗⲴ Southern ᵲӔ࠶᷀ 161 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 1˅н਼цԓ䰤 T-DNA っᇊᙗⲴ Southern ᵲӔ࠶᷀ ޡ䘋㹼Ҷ 5 њцԓᶀᯉ T-DNA ᨂޕっᇊᙗⲴ Southern ᵲӔ࠶᷀DŽ䘉 5 њц ԓवᤜ LH198 BC3F3 ǃ LH198 BC3F4 ǃ LH198 BC3F6 ǃ LH198 BC3F7 ઼ ˄LH198 BC3F7 × LH287˅F1˄䱴മ 11˅ˈަѝ LH198 BC3F4 ᱟ⭘ᶕ䘋㹼࠶ᆀ ⢩ᖱ⢩ᙗ࠶᷀Ⲵцԓ˄䱴മ 7ǃമ 15 ઼䱴മ 9 ઼䱴മ 10˅DŽ䖜ॆਇփᵲӔ LH198 × HiII ԕ৺ᵲӔ LH198 × LH287 Ѫ Southern ᵲӔⲴሩ➗DŽ䴰㾱ᤷࠪ Ⲵᱟˈቭ㇑㠚 R0 ԓᔰ䖜ॆἽṚᔰ⭘ LH198 䘋㹼ཊԓഎӔˈަ䚇Ր㛼Ჟสᵜ Ҿ LH198ˈն❦ਟԕ㛟ᇊަ䚇Ր㛼Ჟѝᴹਟ㜭ਜ਼ᴹ HiII˄∄ྲ൘ BC3F4ੁٮ цԓਜ਼ᴹ 94%Ⲵ LH198 䚇Ր㛼Ჟ઼ 6%Ⲵ HiII 䚇Ր㛼Ჟ˅DŽ㘳㲁ࡠਟ㜭ᆈ൘Ⲵ Ⓚ㛼Ჟˈ䘹⭘ਇփ LH198 × HiII˄㘼䶎 LH198˅Ѫሩ➗DŽ Ӿ MON 87427 Ⲵн਼цԓᶀᯉԕ৺ሩ➗ᶀᯉᨀਆ DNA˄LH198 BC3F3ǃ LH198 BC3F6 ઼ LH198 BC3F7 Ⲵ DNA ᱟӾਦ⡷ᨀਆˈަᆳцԓ DNA ᱟӾᆀ ѝᨀਆⲴ˅ˈ㓿䲀ࡦᙗ࠷䞦 Nsi I ⎸ॆ˄䱴മ 6˅ˈ⭘ PV-ZMAP1043 䍘㋂Ⲵ᧒䪸 1 ઼᧒䪸 4˄䱴മ ˅䘋㹼 Southern ᵲӔDŽྲ᷌ T-DNA ᨂޕ൘цԓ䰤н㜭ཏっᇊ 䚇ՐˈࡉՊ൘ Southern ᵲӔ㔃᷌ѝࠪ⧠䶎亴ᵏᶑᑖ˄亴ᵏᶑᑖ㿱䱴മ 6 ઼䱴㺘 4˅DŽ⭘᧒䪸 1 ઼᧒䪸 4 ᵲӔⲴ㔃᷌㿱䱴മ 12DŽ ᰐ䇪ᱟሩ➗䘈ᱟ MON 87427 䜭൘~4.3 kb ༴Ự⍻ࡠаᶑᵲӔᶑᑖ˄䱴മ 12ˈ 䚃 1~10˅ˈഐ↔ਟԕ䇔Ѫ䈕ᵲӔᶑᑖҾ⦹㊣Ⓚ㛼ᲟⲴᵲӔؑਧˈ㘼о⌣ MON 87427 Ⲵᨂޕ DNA ᰐޣDŽ ሩ➗ LH198 × HiII สഐ㓴 DNA ⭘ Nsi I ⎸ॆਾо᧒䪸⁑ᶯ˄᧒䪸 1 ઼᧒䪸 4˅ ਸˈ䲔~4.3 kb ⲴⓀᙗᵲӔᶑᑖཆˈ䘈ӗ⭏ҶⓀҾ䍘㋂ PV-ZMAP1043 Ⲵ ~1.2 kb ઼~0.7 kb 亴ᵏབྷሿᶑᑖ˄䱴മ 12ˈ⌣䚃 1 ઼ 2˅DŽᑨ㿴ሩ➗ LH198 × HiII ˈสഐ㓴 DNA ⭘Nsi I⎸ॆਾо亴ݸ⭘ Sph I⎸ॆⲴ䍘㋂䖭փPV-ZMAP1043 ਸ 䲔~4.3 kb ⲴⓀᙗᵲӔᶑᑖཆˈ䘈൘~7.1 kb ઼~1.8 kb ༴ӗ⭏ 2 ᶑ亴ᵏབྷሿⲴᶑ ᑖ˄䱴മ 12ˈ⌣䚃 3˅DŽ䱣ᙗሩ➗ⲴỰࠪ㺘᰾᧒䪸оަ䶦ḷᒿࡇਟԕᵲӔDŽ Ӿ LH198 BC3F3ǃLH198 BC3F4ǃLH198 BC3F6ǃLH198 BC3F7 ઼˄ LH198 BC3F7 × LH287˅F1 цԓᨀਆⲴ DNA ⭘ Nsi I ⎸ॆ˄䱴മ 12ˈ⌣䚃 5ǃ6ǃ7ǃ8 ઼ 10˅ˈ䲔к䘠ⓀᙗᵲӔᶑᑖཆˈ䘈൘~6.4 kbǃ~2.0 kb ઼~1.7 kb ༴ӗ⭏ 3 ᶑ 亴ᵏᶑᑖ˄䱴㺘 4˅DŽ~1.7 kb ᶑᑖԓ㺘Ҷᨂޕ T-DNA Ⲵ 5’ᵛㄟԕ৺аሿ䜘࠶䛫 㘬สഐ㓴 DNAˈㅖ ਸ ~1.7 kb 䗩⭼⡷⇥Ⲵ亴ᵏབྷሿ˗~2.0 kb ᶑᑖਜ਼ᴹа䜘ח᧕Ⲵ 㘬䛫חㄟ’3 ޕDNA 䜘ᒿࡇDŽ~6.4 kb ᶑᑖԓ㺘 T-DNA Ⲵ 3’ᵛㄟԕ৺ᨂ ޕ࠶ᨂ ᧕Ⲵสഐ㓴 DNA ˈㅖਸ @ \ 䗩⭼⡷⇥Ⲵ亴ᵏབྷሿDŽMON 87427 Ⲵ LH198 BC3F3ǃLH198 BC3F6ǃLH198 BC3F7 ઼˄LH198 BC3F7 × LH287˅F1 цԓ˄䱴മ 12ˈ⌣䚃 5ǃ6ǃ7ǃ8 ઼ 10˅ӗ⭏Ⲵᑖ䉡⢩ᖱо LH198 BC3F4 цԓ ਼˄䱴മ 7ˈ⌣䚃 4 ઼ 11˅ˈ㺘᰾ MON 87427 ѝঅњᤧ䍍Ⲵ T-DNA ൘ᡰ䘹 цԓѝਟԕっᇊ䚇ՐDŽ 162 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䘋㹼୶ъॆ ૱䖜㛢 TI: [RP×LH198 BC3F4] BC0F1 ×RP ˅TI: BC1F1 ˄࠶∄ֻ 1:1 ×RP ˅TI: BC2F1 ˄࠶∄ֻ 1:1 ! ˅TI: BC2F2 ˄࠶∄ֻ 3:1 䱴മ11. MON 87427㛢㌫䉡മ Ӿ LH198 BC3F2 ѝ䘹ᤙаṚᣇ㥹⭈㟖ἽṚ䘋а↕㠚Ӕਟԕᗇࡠ LH198 BC3F3ˈ 䘹ᤙ LH198 BC3F3 ѝ㓟ਸփ㠚Ӕᗇࡠ LH198 BC3F4DŽLH198 BC3F4 цԓ⭘Ҿ࠶ᆀ ᖱ⢩ᙗ࠶᷀ˈᒦ⭘䘋а↕എӔ䖜㛢ษ㛢ਟ୶ъॆᓄ⭘Ⲵ૱DŽLH198 BC3F3ǃ⢩ LH198 BC3F4ǃLH198 BC3F6ǃLH198 BC3F7 ઼[LH198 BC3F7 × LH287] F1 цԓ ˄࣐㋇ᱮ⽪˅㻛⭘Ҿཊцԓ䚇Րっᇊᙗ࠶᷀DŽ[LH198 BC3F7 × LH287] F1 цԓ⭘ Ҿ䘋㹼㳻ⲭ㺘䗮䟿ǃᡀ࠶ǃ㺘රᙗ⣦ǃߌ㢪ᙗ⣦ԕ৺⧟ຳӂㅹ࠶᷀DŽ RP × LH198 BC3F4 ⲴഎӔ઼㠚Ӕਾԓᶀᯉ⭘Ҿᆏᗧቄ䚇Րᆖ࠶᷀DŽ˄F#Ѫ㠚Ӕц ԓ˗ Ѫ㠚Ӕ˗BC#ѪഎӔцԓDŽ˅ 163 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴മ 12. MON 87427ᨂޕᒿࡇ൘ཊцԓ䰤っᇊⲴSouthern࠶᷀˖᧒䪸1઼᧒䪸4 ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢DŽ᧒䪸 1 ઼᧒䪸 4 ӵ㾶ⴆ䜘࠶ T-DNA˄䱴 മ઼മ13˅DŽLH198 BC3F3ǃLH198 BC3F6 ઼ LH198 BC3F7 Ⲵ DNA ᱟӾਦ⡷ ᨀਆˈަᆳцԓ DNA ᱟӾᆀѝᨀਆⲴDŽ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ࠷䞦⎸ ॆⲴ DNA ṧ૱DŽ⌣䚃䈤᰾ྲл˄ᤜਧ㺘⽪⭘Ҿ⎸ॆⲴ࠷䞦˅˖ ⌣䚃 ṧ૱᧿䘠 [᧒䪸 1 ઼᧒䪸 4[ᖃҾ~0.1 ؽสഐ㓴 DNAޕሩ➗ LH198 × HiII (Nsi I) 1 [᧒䪸 1 ઼᧒䪸 4[ᖃҾ~1.0 ؽสഐ㓴 DNAޕሩ➗ LH198 × HiII (Nsi I) 2 [䞦࠷䍘㋂ (Sph I) [ᖃҾ~1.0 ؽสഐ㓴 DNAޕሩ➗ LH198 × HiII (Nsi I) 3 4 ሩ➗ LH198 × HiII (Nsi I) 5 MON 87427 (LH198 BC3F3) (Nsi I) 6 MON 87427 (LH198 BC3F4) (Nsi I) 7 MON 87427 (LH198 BC3F6) (Nsi I) 8 MON 87427 (LH198 BC3F7) (Nsi I) 9 ሩ➗ LH198 × LH287 (Nsi I) 10 MON 87427([LH198 BC3F7 × LH287] F1) (Nsi I) ㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠 164 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2˅MON 87427 ሬޕᙗ⣦Ⲵ䚇Ր࠶ ൘ MON 87427 Ⲵ⹄ウᔰਁ䗷〻ѝˈ䘋㹼ҶޣⲴ䚇Ր࠶䈅傼ˈԕ䇴ՠ MON 87427 ѝᨂޕⲴ cp4 epsps สഐ㺘䗮ⴂⲴ䚇ՐっᇊᙗDŽ࡙⭘ᯩ⍻傼ᶕ∄䖳 ᇎ䱵࠶∄઼ֻᦞᆏᗧቄ䚇Ր㿴ᖻ䇑㇇Ⲵ亴ᵏ࠶∄ֻⲴᤏਸ〻ᓖDŽ ᯩⲴ䇑㇇ޜᔿѪ˖ 02 ` 5 |} M ަѝ˖o= ᇎ䱵㿲ሏࡠⲴสഐරᡆ㺘⧠ර仁⦷˗e= ⨶䇪亴ᵏสഐරᡆ㺘⧠ර仁⦷˗ 㔏䇑ᱮ㪇≤ᒣ䇮ᇊѪ ` DŽ 䖜ॆᗇࡠⲴ R0 ԓἽṚ⭘ LH198 എӔࠐԓਟԕᗇࡠᵲਸⲴ LH198 BC3F1 ц ԓDŽሶ LH198 BC3F1 㠚Ӕਟԕᗇࡠ࠶㗔փ LH198 BC3F2DŽӾ LH198 BC3F2 ѝ䘹ᤙаṚᣇ㥹⭈㟖ἽṚ䘋а↕㠚Ӕਟԕᗇࡠ LH198 BC3F3 ˈ䘹ᤙ LH198 BC3F3 ѝ㓟ਸփ㠚Ӕᗇࡠ LH198 BC3F4˄䱴മ 11˅DŽLH198 BC3F4 Ⲵ ᆀ⭘ҾഎӔ䖜㛢ᶕ䘋а↕ᔰਁ୶ъॆⲴ૱DŽާփᱟ⭘нਜ਼ cp4 epsps สഐ㺘䗮 ⴂⲴӢᵜ˄〠Ѫ䖞എӢᵜˈRP ˅എӔDŽഎӔᖃԓѪᵲਸ㗔փˈ〠Ѫ ˄RP × LH198 BC3F4˅BC0F1DŽ䘋а↕എӔᗇࡠ˄RP × LH198 BC3F4˅BC1F1ˈ ሩަ௧ᯭ㥹⭈㟖ਟԕ䘹ᤙᣇ㥹⭈㟖ⲴἽṚDŽ⭘ᣇᙗἽṚഎӔᗇࡠ BC2F1ˈ൘ BC2F1 цԓ䘹ᤙᣇᙗἽṚ㠚Ӕᗇࡠ BC2F2 ԓDŽ൘ BC1F1ǃBC2F1 ઼ BC2F2 ц ⨶ԓ䈳ḕ㥹⭈㟖ᣇᙗἽṚⲴ࠶∄ֻˈBC1F1 ઼ BC2F1 ⲴᣇᙗἽṚ:ᝏἽṚⲴ 䇪࠶∄ֻѪ 1:1ˈ㘼 BC2F2 Ⲵ⨶䇪࠶∄ֻѪ 3:1˄䱴മ 11˅DŽ䈳ḕⲴἽṚᮠǃ 䇪࠶∄ֻ৺ަᯩ⍻傼㔃᷌㿱䱴㺘 5DŽӾ䘉Ӌ㔃᷌ਟⴻࠪˈMON 87427 Ⲵᣇ⨶ 㥹⭈㟖ᙗ⣦ㅖਸᆏᗧቄঅสഐ䚇Ր㿴ᖻDŽӾ㘼䇱ᇎ MON 87427 ѝⲴ cp4 epsps สഐ㺘䗮ⴂ൘অаս⛩кӵᴹঅᤧ䍍ᨂޕˈᒦਟ൘ਾԓѝっᇊ䚇ՐDŽ ֻ∄䱴㺘5. MON 87427Ⲵ㓴㓷⢩ᔲᙗ㥹⭈㟖ᣇᙗⲴ䚇Ր࠶ 㿲ሏἽṚ 㿲ሏࡠ 㿲ሏࡠ 亴ᵏⲴ 亴ᵏⲴ цԓ 1 2 ᱮ㪇ᙗ ᙫᮠ 2 䱣ᙗṚᮠ 䱤ᙗṚᮠ 䱣ᙗṚᮠ 䱤ᙗṚᮠ BC1F1 238 109 129 119 119 1.6807 >0.05 BC2F1 290 145 145 145 145 0 >0.05 BC2F2 1107 820 287 830 277 0.5062 >0.05 1 ᡰ䘹⭘цԓᶀᯉⲴ䉡㌫ޣ㌫䈧৲㿱മ 18˗ 2 䇴ԧⲴἽṚᙫᮠˈަѝ䱣ᙗṚѪ㥹⭈㟖㙀ਇරἽṚˈ䱤ᙗṚѪ㥹⭈㟖ᝏරἽṚDŽ 3˅MON 87427 н਼цԓᶀᯉ CP4 EPSPS 㺘䗮Ⲵ Western ࠶᷀ ࡽ 2.6.3 䜘࠶ᡰ䘠ˈ֯⭘ Western ᯩ⌅࠶᷀ MON 87427 㛢䉡㌫Ⲵ 5 њцྲ ԓᶀᯉˈवᤜ˖LH198 BC3F3 цԓⲴਦ⡷઼ LH198 BC3F4ǃLH198 BC3F6ǃ LH198 BC3F7 ઼˄LH198 BC3F7 × LH287˅F1 цԓⲴᆀ˗ሩ➗ᶀᯉѪਇփ LH198 × HiII ⲴᆀDŽ࠶᷀㔃᷌㺘᰾ CP4 EPSPS 㳻ⲭ൘ MON 87427 㛢䉡㌫Ⲵ 5 њцԓᶀᯉѝ൷ਟԕっᇊ㺘䗮˄䱴മ 13˅DŽ 165 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3-7 ᨂޕ⡷⇥㺘䗮ӗ⢙Ⲵ㺘䗮䟿࠶᷀ 㓴㓷઼ಘᇈѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭⲴᇊ䟿ᱟ䙊䗷䞦㚄ݽޣMON 87427 ⯛˄ELISA˅Ⲵᯩ⌅ᆼᡀⲴDŽMON 87427 Ⲵᶀᯉṧ૱ᱟӾ 2008 ᒤ൘㖾ഭ䘋㹼Ⲵ ⭠䰤䈅傼ѝ䟷䳶Ⲵˈޡ 5 њ䈅傼⛩˄࠶࡛սҾսҾ㖾ഭ⦹㊣ѫӗ४Ⲵ䱯㛟㢢ᐎ Jackcon 䜑ǃ⡡㦧ॾᐎⲴ Jefferson 䜑ǃԺ࡙䈪ԺᐎⲴ Stark 䜑ǃঠㅜᆹ㓣ᐎⲴ Parker 䜑ԕ৺ᐳᯟ࣐ᐎⲴ York 䜑˅ˈ⇿њ䈅傼⛩Ⲵ䈅傼Ѫ 3 ⅑䟽༽ˈਆṧ൘ᡰᴹ 䈅傼⛩Ⲵᡰᴹ䟽༽䘋㹼DŽਆṧ઼࠶᷀Ⲵᶀᯉवᤜн਼⭏䮯䱦⇥䟷䳶Ⲵ MON 87427 㓴㓷ṧ૱㊫රޡ䇑 19 ˖ޘ⭏㛢ᵏⲴਦ⡷˄OSL1~4˅ǃ㊭㋂ǃ㣡 ㊹ǃ㣡эǃൠк䜘ᮤṚǃ㤾ਦǃޘ⭏㛢ᵏṩ㌫˄OSR1~4˅ǃ≄⭏ṩǃ㺠㘱ṩ㌫ ԕ৺ޘ⭏㛢ᵏᮤṚ˄OSWP1~4˅DŽ ELISA ࠶᷀㔃᷌≷ᙫҾ䱴㺘 6DŽMON 87427 Ⲵн਼㓴㓷઼ಘᇈѝ CP4 EPSPS 940 μg/g dwt 㳻ⲭⲴ㺘䗮䟿ਈॆ㤳ത䖳བྷˈӾᴰሿ٬վҾỰࠪ䲀˄LOD˅ࡠᴰབྷ٬ ᗞݻ/ݻ㓴㓷ᒢ䟽˅DŽަѝਦ⡷ѝⲴᒣ൷㺘䗮䟿ᴰ儈˄㤳തӾ OSL3 Ⲵ˄ 㤳തӾ OSWP4 Ⲵ˄μg/g dwt ࡠ OSL1 Ⲵ 680 μg/g dwt˅˗ަ⅑ᱟᮤṚᒣ൷٬ 290 μg/g dwt ࡠ OSWP1 Ⲵ 500 μg/g dwt˅DŽ䲿ਾ⅑Ѫṩ㌫˄Ӿ OSR3 Ⲵ 240 73 μg/g dwt ࡠ OSR1 Ⲵ 140 μg/g dwt˅ǃൠк䜘ᮤṚ˄120 μg/g dwt˅ǃ㣡э ˄100 μg/g dwt˅ǃ≄⭏ṩ˄72 μg/g dwt˅ǃ㺠㘱ṩ㌫˄72 μg/g dwt˅ǃ㤾ਦ˄43 μg/g dwt˅ ઼㊭㋂˄4.2 μg/g dwt˅DŽ ᴹ 6 њ MON 87427 㣡㊹ṧ૱˄ᶕ㠚 3 њ䈅傼⛩˅Ⲵ CP4 EPSPS 㳻ⲭ㺘䗮䟿 վҾỰࠪ䲀˄ 166 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴㺘 6. 2008 ᒤ൘㖾ഭ⭠䰤䟷䳶Ⲵ MON 87427 㓴㓷ṧ૱ѝ CP4 EPSPS 㳻ⲭਜ਼䟿 ˅SD˄SD˅ ᒣ൷٬˄ᒣ൷٬ ਾⲴཙ LOD/LOQ4 㓴㓷㊫ර ਁ㛢ᰦᵏ 1 㤳ത 㤳ത ᮠ˄DAP˅ 2 3 ਦ⡷ OSL1 V2-V5 20-28 100 (21) 680 (170) 0.069/0.137 75 – 140 400 – 940 OSL2 V6-V8 32-46 83 (25) 410 (130) 0.069/0.137 30 –110 130 – 560 OSL3 V10-V12 41-67 61 (19) 290 (74) 0.069/0.137 35 –95 210 – 410 OSL4 VT 54-73 95 (30) 370 (120) 0.069/0.137 17 – 140 70 – 520 ㊭㋂ R6 118-182 3.6 (0.73) 4.2 (0.89) 0.16/0.228 2.6 – 5.3 2.8 – 6.2 㣡㊹ 5 ᦸ㊹ᵏ 58-81 < LOD (NA) 㣡э ᦸ㊹ᵏ 58-76 9.4 (0.97) 100 (12) 0.121/0.137 8.1 – 11 90 – 120 ൠк䜘ᮤṚ R5 83-116 38 (14) 120 (48) 0.069/0.137 8.3 – 57 21 – 200 㤾ਦ R6 124-180 14 (6.3) 43 (27) 0.069/0.137 5.9 – 26 13 – 98 ṩ㌫ OSR1 V2-V5 22-28 18 (5.3) 140 (46) 0.033/0.068 8.1 – 27 58 – 210 OSR2 V6-V8 32-46 16 (6.8) 110 (62) 0.033/0.068 8.3 –29 48 – 240 OSR3 V10-V12 41-67 12 (4.3) 73 (28) 0.033/0.068 4.9 – 19 22 – 110 OSR4 VT 54-73 15 (5.7) 83 (36) 0.033/0.068 5.6 – 23 23 – 140 167 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅SD˄SD˅ ᒣ൷٬˄ᒣ൷٬ ਾⲴཙ LOD/LOQ4 㓴㓷㊫ර ਁ㛢ᰦᵏ 1 㤳ത 㤳ത ᮠ˄DAP˅ 2 3 ≄⭏ṩ R5 83-116 15 (5.2) 72 (23) 0.033/0.068 8.6 – 24 39 – 100 㺠㘱ṩ㌫ R6 124-180 16 (8.3) 72 (37) 0.033/0.068 5.9 – 29 26 – 130 ᮤṚ OSWP1 V2-V5 22-28 50 (8.3) 500 (190) 0.069/0.137 37 – 66 310 – 840 OSWP2 V6-V8 32-46 46 (7.6) 360 (42) 0.069/0.137 33 – 58 300 – 420 OSWP3 V10-V12 41-67 43 (7.1) 380 (78) 0.069/0.137 28 – 56 230 – 500 OSWP4 VT 54-73 37 (6.3) 240 (42) 0.069/0.137 23 – 47 160 – 340 1 䟷ṧᰦ⦹㊣ᡰ༴Ⲵਁ㛢ᰦᵏDŽ ᴰབྷ-ݻ勌䟽㓴㓷˄fwt˅ѝਜ਼ᴹⲴᗞݻᮠˈᒣ൷٬ǃḷ߶ᐞ˄SD˅઼㤳ത˄ᴰሿ٬⇿ 2 ᱟṩᦞᡰᴹ䈅傼⛩Ⲵṧ૱ᮠᦞ䇑㇇ᶕⲴ˄≄⭏ṩṧ૱ n=11ˈ㣡㊹ṧ૱ n=6ˈަᆳ˅٬ ṧ૱ n=14˅DŽ ݻᒢ䟽㓴㓷˄dwt˅ѝਜ਼ᴹⲴᗞݻᮠˈᒢ䟽٬ᱟṩᦞ勌䟽٬䲔ԕᒢ䟽䖜ᦒ㌫ᮠ㘼ᗇDŽ⇿ 3 4 LOQ=ᇊ䟿䲀ˈLOD=Ựࠪ䲀DŽ 5 MON 87427 㣡㊹ṧ૱Ⲵ CP4 EPSPS 㳻ⲭ≤ᒣᡆ㘵վҾỰࠪ䲀˄LODˈ ˈn=6˅ˈ ᡆ㘵ӵ⮕儈Ҿᇊ䟿䲀˄LOQˈ ˈn=6˅DŽ 168 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3-8 ᨂޕ⡷⇥㺘䗮ӗ⢙൘ཊњцԓѝⲴ㺘䗮っᇊᙗ࠶᷀ MON 87427 ᱟ䙊䗷ߌᵶ㧼ӻሬ䖜ॆᯩ⌅ሶ PV-ZMAP1043 䍘㋂Ⲵ T-DNA ሬ ޕࡠਇփ⦹㊣ LH198 × HiII ѝ㧧ᗇⲴDŽLH198 × HiII ᱟњᵲӔˈަѝӢᵜ LH198 ḃ∄䖳ᝏ৸᱃Ҿ࠶ॆ⭏ˈᱟᑨ⭘ץᴹ㢟ྭⲴߌ㢪ᙗ⣦ˈ㘼 HiII ሩߌᵶ㧼ާ Ⲵ⦹㊣䖜ॆਇփDŽ㧧ᗇⲴ R0 ԓἽṚ䙊䗷оߌ㢪ᙗ⣦䖳ྭⲴ LH198 എӔཊԓˈᒦ 㓿䘎㔝㠚ᵲˈᴰ㓸㧧ᗇ MON 87427DŽMON 87427 Ⲵ䘹㛢䗷〻৺㛢മ䉡㿱䱴മ 11DŽ ᨂޕᒿࡇ㺘䗮っᇊᙗ࠶᷀वᤜҶ MON 87427 Ⲵ 5 њцԓᶀᯉ˄䱴മ 11˅˖ LH198 BC3F3 цԓⲴਦ⡷઼ LH198 BC3F4ǃLH198 BC3F6ǃLH198 BC3F7 ઼ ˄LH198 BC3F7 × LH287˅F1 цԓⲴᆀ˗ሩ➗ᶀᯉѪਇփ LH198 × HiII Ⲵ ᆀDŽ䴰㾱ᤷࠪⲴᱟˈቭ㇑㠚 R0 ԓᔰ䖜ॆἽṚᔰ⭘ LH198 䘋㹼ཊԓഎӔˈަ ∄˄Ҿ LH198ˈն❦ਟԕ㛟ᇊަ䚇Ր㛼Ჟѝᴹਟ㜭ਜ਼ᴹ HiIIੁٮ䚇Ր㛼Ჟสᵜ ྲ൘ BC3F4 цԓਜ਼ᴹ 94%Ⲵ LH198 䚇Ր㛼Ჟ઼ 6%Ⲵ HiII 䚇Ր㛼Ჟ˅DŽ㘳㲁ࡠਟ 㜭ᆈ൘ⲴⓀ㛼Ჟˈ䘹⭘ਇփ LH198 × HiII Ѫሩ➗DŽབྷ㛐ᵶ㧼㺘䗮Ⲵ CP4 EPSPS 㳻ⲭ˄2ng˅Ѫḷ߶৲➗㳻ⲭDŽ ࡙⭘ CP EPSPS 㳻ⲭⲴ Western ঠ䘩࠶᷀ᶕ䇱ᇎ MON 87427 ѝᨂޕᒿࡇ㺘䗮 ӗ⢙൘ཊњцԓѝⲴっᇊᙗˈWestern ࠶᷀㔃᷌㿱䱴മ 13DŽ↓ྲ亴ᵏ㔃᷌аṧˈ MON 87427 Ⲵཊњцԓᶀᯉ൷ਟỰ⍻ࡠ CP4 EPSPS 㳻ⲭᶑᑖ˄䱴മ 13ˈ⌣䚃 DŽLH198 BC3F3 цԓਦ⡷ᶀᯉⲴ Western ݽ⯛ؑਧ˄䱴മ 13ˈ⌣䚃 4˅᰾ᱮ˅8~4 ᕪҾަᆳцԓⲴᆀᶀᯉˈ䈤᰾ CP4 EPSPS 㳻ⲭ൘ਦ⡷ѝⲴ㺘䗮䟿᰾ᱮ儈Ҿ ᆀˈ䘉о CP4 EPSPS 㺘䗮䟿Ⲵ ELISA Ự⍻㔃᷌ᱟа㠤Ⲵ˄䱴㺘 6˅DŽᡰᴹ䘉Ӌ ᶑᑖⲴ䗱〫⦷оབྷ㛐ᵶ㧼㺘䗮 CP4 EPSPS 㳻ⲭⲴ䗱〫⦷˄䱴മ 13ˈ⌣䚃 3˅ᱟ а㠤Ⲵˈ䇱ᇎҶ Western ঠ䘩ѝỰ⍻ࡠⲴ MON 87427 цԓᶀᯉݽ⯛ᶑᑖ⺞ᇎ ᱟ CP4 EPSPS 㳻ⲭˈᒦфњцԓᆀᶀᯉѝ㺘䗮䟿լ˄䱴മ 13ˈ⌣䚃 5~8˅DŽ Ѫ䱤ᙗሩ➗ˈᑨ㿴ሩ➗ᶀᯉѝ⋑ᴹỰ⍻ࡠ CP4 EPSPS 㳻ⲭ˄䱴മ 13ˈ⌣䚃 10˅DŽ Western ࠶᷀㔃᷌㺘᰾ˈMON 87427 Ⲵ CP4 EPSPS 㳻ⲭ൘н਼䚇Րцԓ䰤 ਟԕっᇊ㺘䗮DŽ 169 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴മ13. MON 87427ཊњцԓᶀᯉѝCP4 EPSPS㳻ⲭⲴWestern࠶᷀ MON 87427 Ⲵ 4 њцԓᆀᶀᯉ઼ 1 њцԓਦ⡷ᶀᯉⲴㅹ䟿ᨀਆ⢙ԕ ৺࠶ᆀ䟿ḷ䇠䟷⭘ SDS-PAGE ࠶ˈ❦ਾ⭥䖜〫㠣 PVDF 㟌DŽሶ㟌оኡ㖺 ˖ᣇ CP4 EPSPS ᣇփᆥ㛢ˈ⭘ॆᆖਁݹ䈅ࡲᱮ㢢DŽ⌣䚃䈤᰾ྲл ⌣䚃 ᧿䘠 ࠍ㜦кṧ䟿 1 オⲭ -- 2 ࠶ᆀ䟿ḷ䇠˄Magic Marker˅ 0.5 l 3 བྷ㛐ᵶ㧼˄E. coli˅㺘䗮Ⲵ CP4 EPSPS 㳻ⲭ˄2ng˅ 2ng 4 LH198 BC3F3˄ਦ⡷˅ 20 l 5 LH198 BC3F4˄ᆀ˅ 20 l 6 LH198 BC3F6˄ᆀ˅ 20 l 7 LH198 BC3F7˄ᆀ˅ 20 l 8 (LH198 BC3F7 × LH287)F1˄ᆀ˅ 20 l 9 オⲭ -- 10 ᑨ㿴ሩ➗ LH198 × HiII˄ᆀˈᢩਧ 11214241-004˅ 20 l 170 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 4. 䖢ะഖᙝ⣬ӝ⢟ⲺỶ⎁ૂ䢪ᇐᢶᵥ 4-1. MON 87427 䖜ॆփ⢩ᔲᙗࠍ㜦ᇊᙗ PCR Ự⍻ᯩ⌅˄CBI˅ 1˅ࠍ㜦ᇊᙗ PCR Ự⍻ᕅ⢙ ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ 2˅৽ᓄփ㌫઼৽ᓄ〻ᒿ MON 87427 ࠍ㜦ᇊᙗ PCR ᢙ໎৽ᓄփ㌫˖ ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ hmgA ࠍ㜦ᇊᙗ PCR ᢙ໎৽ᓄփ㌫˖ ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ 2˅৽ᓄ〻ᒿ ᇊᙗ PCR ᢙ໎৽ᓄᗚ⧟ᶑԦ˄䘲⭘Ҿ MON 87427 ઼ hmgA PCR ৽ᓄ˅˖ ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ 3˅PCR 㔃᷌Ⲵਟ᧕ਇḷ߶ a. ᐢ⸕Ⲵ䱣ᙗṧ૱ᗵ享ӗ⭏亴ᵏⲴ PCR ӗ⢙˗ b. ᰐ⁑ᶯሩ➗઼ަᆳᐢ⸕Ⲵ䱤ᙗṧ૱ᗵ享Ự⍻нࡠ亴ᵏབྷሿⲴ PCR ӗ⢙DŽ 171 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 4-2. MON 87427 䖜ॆփ⢩ᔲᙗᇎᰦᇊ䟿 TaqMan® PCR Ự⍻ᯩ⌅˄CBI˅ 1˅ᇊ䟿 PCR Ự⍻ᕅ⢙ ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ 2˅৽ᓄփ㌫઼৽ᓄ〻ᒿ MON 87427 Ⲵ PCR ৽ᓄਸ⏢˄অњ৽ᓄ˅ ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ hmgA Ⲵ PCR ৽ᓄਸ⏢˄অњ৽ᓄ˅ ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ ᇊ䟿 PCR ᢙ໎৽ᓄᗚ⧟ᶑԦ˄䘲⭘Ҿ MON 87427 ઼ hmgA PCR ৽ᓄ˅ ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ 3˅ᇊ䟿ḷ߶ᴢ㓯Ⲵ㔈ࡦ઼䇑㇇ ሶ㓟ॆⲴᐢ⸕⎃ᓖ MON 87427 สഐ㓴 DNA ⓦ⏢оᐢ⸕⎃ᓖᑨ㿴⦹㊣สഐ 㓴 DNA ⓦ⏢ਸᶕࡦ༷ MON 87427 ḷ߶ DNA ⓦ⏢DŽMON 87427 Ⲵ DNA ḷ߶ ⓦ⏢⎃ᓖ࠶࡛Ѫ 50.0ǃ25.0ǃ5.0ǃ2.5ǃ0.5ǃ0.25ǃ0.05ǃ0.025 ઼ ˗ᓄ Ⲵ MON 87427 สഐ㓴 DNA ൘ᙫ⦹㊣สഐ㓴 DNA ѝᡰঐ∄ֻ࠶࡛Ѫ 100%ǃ50%ǃ 10%ǃ5%ǃ1%ǃ0.5%ǃ0.1%ǃ0.05%઼ 0DŽᡰᴹḷ߶ DNA ⓦ⏢ѝᙫⲴ⦹㊣ DNA ⎃ᓖᓄ䈕Ѫ DŽ ᑨ㿴⦹㊣Ⲵḷ߶ DNA ⓦ⏢䟷⭘ሶ㓟ॆⲴᐢ⸕ᑨ㿴⦹㊣สഐ㓴 DNA оᐢ⸕ Ⲵᑨ㿴ަᆳ⢙˄ྲỹ㣡ǃሿ哖ǃབྷ䉶ㅹ˅สഐ㓴 DNA ਸᶕࡦ༷DŽᑨ㿴⦹㊣ ḷ߶ DNA Ⲵ⎃ᓖ࠶࡛Ѫ 50.0ǃ25.0ǃ12.5ǃ5.0ǃ2.5 ઼ ˗ᓄⲴ⦹㊣ส ഐ㓴 DNA ൘ᙫἽ⢙สഐ㓴 DNA ѝᡰঐ∄ֻ࠶࡛Ѫ 100%ǃ50%ǃ25%ǃ10%ǃ 5%ǃ1%઼ 0DŽᡰᴹḷ߶ⓦ⏢ѝᙫⲴἽ⢙ DNA ⎃ᓖᓄ䈕Ѫ DŽ ᐞˈᡰᴹḷ߶ⓦ⏢൷⭡਼аᢩ⅑㓿䗷ٿѪҶ᧗ࡦḷ߶ⓦ⏢䝽ࡦ䗷〻ѝⲴ䈅傼 㦗ݹᇊ䟿Ⲵสഐ㓴 DNA ۘ⏢䝽ࡦӗ⭏DŽ↔ཆ䙊䗷⭘਼аۘ⏢ởᓖ〰䟺ᗇࡠⲴн 䇱њ⎃ᓖḷ߶ⓦ⏢㓸փ〟䏣ཏབྷⲴ؍ᓖᐕ⏢䘋㹼ḷ߶ⓦ⏢䝽ࡦˈԕ৺⎃਼ 172 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᯩ⌅ˈ䚯ݽሿփ〟ਆṧ䙐ᡀⲴਆṧ䈟ᐞDŽ਼ᰦˈ䝽ࡦḷ߶ⓦ⏢ᡰ⭘Ⲵ࣐ṧᷚҏ 䇱ᡰᴹ DNA Ⲵа㠤ᙗˈк䘠ᡰᴹ DNA ḷ߶ⓦ⏢ࡦ؍㾱≲䙊䗷ѕṬṑ߶DŽѪҶ ⭘Ⲵ DNA ۘ⏢ᓄ䈕ᱟ䟷⭘਼ᯩ⌅ᨀਆⲴDŽ༷֯ ࡦ༷ PCR ৽ᓄփ㌫ᰦˈᔪ䇞൘ 37°C ≤⎤ѝ䀓߫䈅ࡲDŽ䈅ࡲаᰖ䀓߫ˈᔪ䇞 䇱৽ᓄ䰤Ⲵ਼аᙗ઼ሶӔ৹⊑ḃᴰሿ؍ᆈˈᒦ֯⭘ѻࡽᖫᓅरDŽѪҶ؍൘ߠк ॆˈ䲔Ҷ DNA ⁑ᶯঅ⤜࣐ޕࡠ⇿њ৽ᓄփ㌫ѝཆˈє৽ᓄਸ⏢˄ањᱟѪ MON 87427 Ự⍻㘼ਖањᱟѪ hmgA Ự⍻˅ѝⲴަԆ䈅ࡲ㾱᤹➗䏣ཏޘ䜘ᢙ໎ ањ৽ᓄⲴ䟿˅а⅑ڊ৽ᓄⲴ䟿䇑㇇˄ྲ߶༷ањ৽ᓄਸ⏢ᰦ㾱㠣ቁཊ߶༷ ᙗޘ䜘࣐ޕDŽMON 87427 Ⲵ PCR ઼ hmgA Ⲵ PCR ਟԕн൘਼аཙ䘋㹼DŽ PCR ൘ ABI 7500 ᇎᰦᇊ䟿 PCR Ԛк䘋㹼ˈ᤹➗Ԛಘ⭏ӗল୶ᨀⲴ䈤᰾Җ 䘋㹼DŽ96 ᆄ৽ᓄᶯ⇿ᆄѝ࣐ޕ৽ᓄਸ⢙ˈ❦ਾ⇿њ৽ᓄѝ࣐ޕ䘲䟿Ⲵ DNA ⁑ᶯˈⴆ㍗ⴆᆀDŽѪҶᇊ䟿Ự⍻ṧ૱ѝ MON 87427 Ⲵ DNA ਜ਼䟿ˈ㾱ሩᡰᴹ MON 87427 ḷ߶ DNA ⓦ⏢䘋㹼 MON 87427 Ⲵ PCR ৽ᓄˈ਼ᰦ䘈㾱ሩᑨ㿴⦹㊣ ḷ߶ DNA ⓦ⏢䘋㹼 hmgA Ⲵ PCR ৽ᓄDŽ⇿ԭṧ૱䜭ᓄ䈕䘋㹼к䘠єњ PCR ৽ ᓄDŽ⇿њṧ૱䜭㾱ሩк䘠є PCR Ⲵ㔃᷌䘋㹼࠶᷀DŽ⇿њ PCR 䜭ᓄवᤜ 1 њᐢ ⸕ਜ਼ᴹ 1% MON 87427 Ⲵሩ➗˄ਜ਼ 1% MON 87427 Ⲵ DNA ઼ 99%ᑨ㿴⦹㊣ DNA˅ǃ 1 њᐢ⸕Ⲵᑨ㿴⦹㊣ DNA ሩ➗઼ 1 њᰐ DNA ⁑ᶯⲴሩ➗DŽḷ߶ DNA ⓦ⏢ǃỰ ⍻ṧ૱ԕ৺ሩ➗Ⲵ PCR ৽ᓄ൷ᓄ䇮㖞єњԕкⲴ䟽༽DŽ ࣐ޕᡰᴹ䈅ࡲࡠ৽ᓄᶯਾˈሩަ䘋㹼վ䙏ᗳ˄ᔪ䇞 4°C 㠣ᇔ઼ 250× g лᗳ 1 min˅ˈԕ֯ᡰᴹ⏢փ≷㚊൘৽ᓄᆄᓅ䜘DŽ MON 87427 DNA Ⲵ⎃ᓖ઼ᙫ DNA Ⲵ⎃ᓖ˄ ˅ᱟ࡙⭘ MON 87427 PCR ઼ hmgA PCR ḷ߶ᴢ㓯ᶕ䇑㇇ⲴDŽ ḷ߶ᴢ㓯䙊䗷ሩᓄḷ߶ DNA ⓦ⏢⎃ᓖⲴ log 䖜ᦒ٬оᓄḷ߶ DNA ⓦ⏢ ٬Ⲵ൷٬䘋㹼㓯ᙗഎᖂ㔈ࡦDŽഎᖂᴢ㓯Ⲵᯌ⦷㾱≲൘-3.6 ~ -3.1 ѻ䰤 ⅑䟽༽ Ct 3 ٬㾱 ˄㺘᰾൘䇮ᇊⲴ⎃ᓖ㤳ത 㺘᰾ PCR ᢙ໎᭸⦷൘ 90%~110%ѻ䰤˅ˈR2˄ ㌫˅DŽޣ٬оᓅ⢙⎃ᓖሩᮠѻ䰤ާᴹᖸᕪⲴ㓯ᙗ ˈPCR ৽ᓄᡰᗇ Ct ࡙⭘ྲлᯩ〻䇑㇇ MON 87427 Ⲵ DNA ሩҾᙫ DNA Ⲵሩਜ਼䟿˖ MON 87427 DNA ঐᙫ DNA Ⲵሩਜ਼䟿˄%˅= [MON 87427 DNA ⎃ᓖ/ᙫ DNA Ⲵ⎃ ᓖ] ×100 ࡔᯝањ PCR ᮠᦞᱟਟԕ᧕ਇˈᗵ享┑䏣лࡇḷ߶˖˄1˅ᐢ⸕Ⲵ 1% MON 87427 ሩ➗Ⲵሩਜ਼䟿䇑㇇㔃᷌ᗵ享൘ 0.75%~1.25%ѻ䰤˗˄2˅ᐢ⸕ᑨ㿴 ˈ٬൘ MON 87427 PCR 亴䇮Ⲵ 40 њᗚ⧟ᰐਟỰ⍻ؑਧ ㊣ DNA ሩ➗Ⲵ Ct⦹ ٬൘ MON 87427 PCR ઼ hmg PCR ൘ hmg PCR ѝ<40˗˄3˅ᰐ⁑ᶯ DNA ሩ➗Ⲵ Ct 亴䇮Ⲵ 40 њᗚ⧟൷ᰐਟỰ⍻ؑਧDŽ ⤜・ PCR ᇎ傼ѻ䰤Ⲵ 0 DŽRSD Ѫ൘䟽༽ᶑԦлᗇࡠḷ߶䈟ᐞˈᱟ 䙊䗷ሶḷ߶䈟ᐞ˄SD˅䲔ԕ൷٬ᗇࡠⲴDŽਈᔲ㌫ᮠ˄CV˅ҏᱟ䙊䗷ሶḷ߶䈟ᐞ 䲔ԕ൷٬ᗇࡠⲴˈо RSD ਼ˈRSD о CV 䜭ԕⲮ࠶ᮠⲴᖒᔿ㺘⽪DŽ 173 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 5. 䈋僂䱬⇫ᇗҜⲺགྷদԬδ䘑ਙ䖢ะഖ⭕⢟⭩䈭ᆿޞ䇷Ҝ Ⲻθᵢ亯уມߏε ᵜ⭣䈧Ѫ䘋ਓ䖜สഐ⭏⢙ⴤ᧕⭣䈧ᆹޘ䇱Җˈᵜ亩н䘲⭘DŽ 6. 䈋僂䱬⇫Ⲻᆿޞᙝ䇺ԭ䈋僂ᙱ㔉ᣛδ䘑ਙ䖢ะഖ⭕⢟⭩ 䈭ᆿޞ䇷ҜⲺθᵢ亯уມߏε ᵜ⭣䈧Ѫ䘋ਓ䖜สഐ⭏⢙ⴤ᧕⭣䈧ᆹޘ䇱Җˈᵜ亩н䘲⭘DŽ 7. 䖢ะഖὃ⢟ሯ⭕ᘷ⧥ູᆿޞᙝⲺ㔲ਾ䇺ԭᣛ 䈧㿱л亥lj䖜 cp4 epsps สഐ㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣ MON 87427 Ⲵ⧟ຳ ઼伏⭘ᆹޘᙗ㔬ਸ䇴ԧᣕNJDŽ 8. 伕ᆿޞᙝⲺ㔲ਾ䇺ԭᣛθऻᤢ:Aεᗻ㾷Ⲻࣞ⢟∈⨼䈋僂ᣛχ Bε伕䗽ᮅᙝ䇺ԭ䈋僂ᣛχCεф䶔䖢ะഖὃ⢟∊䖹θެ㩛ޱ ᡆԳᣍ㩛ޱഖᆆ࠼᷆ᣛㅿ 䈧㿱л亥lj䖜 cp4 epsps สഐ㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣ MON 87427 Ⲵ⧟ຳ ઼伏⭘ᆹޘᙗ㔬ਸ䇴ԧᣕNJDŽ 174 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䖢 cp4 epsps ะഖ㓺㓽⢯ᔸᙝᣍ㥿⭎㟜⦿㊩ MON 87427 Ⲻ⧥ູૂ伕⭞ᆿޞᙝ㔲ਾ䇺ԭᣛ жȽ㾷δ䖢䘦䖢ะഖ֒⢟Ⲻ䚍Ֆᙝ⣬Ƚ䈋僂ᒪ䲆Ƚ䇺ԭᡌỶ⎁ḽ㔉䇰ε MON 87427 ⭞Ҽ⢯ᇐⲺࣞᆆ-ᆆ㓺ਾδ e35S-hsp70εθ໔ᕰශ 35S ࣞᆆૂ⦿㊩✣◶㴁ⲳ 70 ะഖⲺㅢжѠᆆȾ䈛ࣞᆆ -ᆆ㓺ਾਥԛ⢯ᔸ ᙝ൦൞㩛ޱ⭕䮵ಞᇎδਬ⡽Ƚ〮ૂṯεૂ䴂ᙝ⭕⇌ಞᇎδਥԛ䘑ж↛ਇ㛨ᡆ〃 ᆆᡌ㊳㋈ૂ㣧ѓⲺ㓺㓽εѣ㺞䗴 CP4 EPSPS 㴁ⲳθֵ䘏ӑ㓺㓽ૂಞᇎਥԛᣍ㥿 ⭎㟜Ⱦռ൞њѠީ䭤Ⲻ䳺ᙝಞᇎδ㣧㊿ቅᆘᆆૂ∗㔈ቸ㓼㜔εѣ㺞䗴䠅ᶷքθࠖ ҄Ỷ⎁уࡦȾഖ↚θMON 87427 Ⲻ䳺ᙝಞᇎуᣍ㥿⭎㟜Ⱦ⭞䈛⢯ᙝਥԛ൞⦿ ㊩〃ᆆ⭕ӝ䗽ぁѣθӰѰࡑ䙖䳺ᙝу㛨θ⭞ӄ⦿㊩ᵸӚ〃〃Ⱦ MON 87427 ᱥ䙐䗽ߒᵼ㨂ԁሲ䖢ौθሼ PV-ZMAP1043 䍞㋈ѣⲺ T-DNA ሲ ޛࡦ LH198 × HiII ։㌱ѣ㧭ᗍⲺȾT-DNA ऻᴿжѠ cp4 epsps ะഖ㺞䗴ⴈθ 䈛ะഖ㺞䗴ⴈऻᤢ e35S ࣞᆆȽhsp70 ⲺᆆȽ CTP2 ਬ㔵։䖢〱㛳ᓅࡍȽ cp4 epsps 㕌⸷ᓅࡍૂ nos 㓾↘ᆆȾ Southern ᵸӚ࠼᷆㺞᱄θT-DNA ӻ൞⦿㊩ะഖ㓺жѠᓝփрᆎ൞ঋ䍓ᨈ Ⲻ MON 87427ޛȾ䲚Ҽ T-DNA ཌθ䍞㋈Ⲻެᆹ䈹ݹԬδऻᤢ僞ᷬᓅࡍε⋗ᴿᨈޛ Ⲻะഖ㓺ᓅࡍѣȾPCRૂDNA ᓅࡍ࠼᷆㔉᷒㺞᱄θ T-DNA ᨈޛⲺ㔉ᶺᱥᇂ᮪ⲺȽ 㘲ᓅࡍȾSouthernם㔉ᶺᱥ↙⺤Ⲻχᒬ⭞ PCR ૂ DNA ᓅࡍ࠼᷆⺤ᇐҼะഖ㓺 ൞ 5 Ѡ㛨〃ьԙ䰪ؓᤷどᇐȾ↚ཌθሲޛᵸӚ࠼᷆䇷ᇔ MON 87427 ѣⲺཌⓆᨈ Ⲻ DNA ᓅࡍ൞ཐьԙ䰪ؓᤷどᇐȾޛᙝ⣬Ⲻ䚍Ֆ࠼⿱ᇔ僂㔉᷒ҕ䇷ᇔᨈޛ CP4 EPSPS 㴁ⲳ䍞⢯ᖷ⢯ᙝ䇺ԭᇯऻᤢφ 1ε⨼ौ⢯ᙝૂࣕ㜳࠼᷆χ2ε ൞уੂ㓺㓽ѣⲺ㺞䗴䠅χ3ε⭕⢟ؗᚥᆜ࠼᷆χ4 ൞⁗ᤕ㛹㛖⏨ᶗԬсⲺ⎾ौχ5ε ✣༺⨼どᇐᙝχ 6εᆿޞᓊ⭞়ਨχԛ 7εሯରҩࣞ⢟▒൞ᙛᙝ∈ᙝᇔ僂Ⱦᡶ ᴿԛр䘏ӑ࠼᷆㔉᷒ൽ㺞᱄ MON 87427 㺞䗴Ⲻ CP4 EPSPS фཝ㛖ᵼ㨂㺞䗴Ⲻ ᓭ⋗ᴿ伄䲟Ⱦڛ⢟CP4 EPSPS 㴁ⲳㅿੂθሯӰૂࣞ ṯᦤ OECD θ࠼᷆Ҽ MON 87427 Ⲻ㊳㋈ૂ〮Ⲻ㩛ޱᡆ࠼Ƚᣍ㩛ޱ ᡆ࠼ૂ↚⭕ԙ䉘ᡆ࠼Ⱦᡆ࠼࠼᷆㔉᷒㺞᱄θ MON 87427 Ⲻ〃ᡆ࠼ф䶔䖢ะഖ ሯ➝Ⲻᡆ࠼ᇔ䍞ㅿੂθᒬъൽ൞ᑮ㿺⦿㊩Ⲻ 99%ᇯ䇮॰䰪Ⱦ 〃ᆆՇⵖૂ㩂ਇ⢯ᙝૂ㣧㊿ᖘᘷ⢯ᖷⲺ䇺զ㔉᷒㺞᱄θMON 87427 уՐ໔ Ⱦ㺞ශᙝ⣬Ƚߒ㢰ᙝ⣬ૂф⧥ູӈ֒⹊ガⲺ⭦ੇٴࣖᵸ㥿ौᡌ䖢Ѱᴿᇩ⭕⢟Ⲻ 䰪䈋僂ӄ 2008 ᒪ൞㗄ള⦿㊩ᑜⲺ 5 Ѡ൦⛯䘑㺂ȾMON 87427 ૂ䶔䖢ะഖሯ➝൞ ࠖ҄ᡶᴿᙝ⣬Ⲻᡶᴿᮦᦤሯ∊рൽуᆎ൞㔕䇗ᆜᱴ㪍ᙝᐤᔸȾሯӄᶷቇᮦᆎ൞㔕 䇗ᆜᱴ㪍ᙝᐤᔸⲺᙝ⣬θӂ㘻䰪Ⲻᐤᔸҕуޭᴿ⭕⢟ᆜᝅѿȾ ӂȽ㜂Ქԁ㔃 ᆕኧ䜳ޢਮᔶਇⲺ MON 87427 ⭞Ҽ⢯ᇐⲺࣞᆆ - ᆆ㓺ਾ δe35S-hsp70εθ໔ᕰශ 35S ࣞᆆૂ⦿㊩✣◶㴁ⲳ 70 ะഖⲺㅢжѠᆆȾ 䈛ࣞᆆ-ᆆ㓺ਾਥԛ⢯ᔸᙝ൦൞㩛ޱ⭕䮵ಞᇎδਬ⡽Ƚ〮ૂṯεૂ䴂ᙝ ⭕⇌ಞᇎδਥԛ䘑ж↛ਇ㛨ᡆ〃ᆆᡌ㊳㋈ૂ㣧ѓⲺ㓺㓽εѣ㺞䗴ߒᵼ㨂 CP4 㨂 175 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ṠⲺ 5-✥䞽я䞤䞦-3-⼭䞮䖢〱䞬δCP4 EPSPSεθֵ䘏ӑ㓺㓽ૂಞᇎਥԛᣍ㥿⭎ 㟜Ⱦռ䘏ж⢯ᇐⲺࣞᆆૂᆆ㓺ਾֵᗍ CP4 EPSPS 㴁ⲳ൞њѠީ䭤Ⲻ䳺ᙝ ಞᇎѣу㺞䗴ᡌ㺞䗴䠅ᴿ䲆φ㣧㊿ቅᆘᆆδਥԛ䘑ж↛ਇ㛨ᡆ㣧㊿㋈εૂ∗㔈ቸ εȾഖ↚θMON 87427 Ⲻ䳺ᙝಞᇎуᣍ㥿⭎㟜Ⱦޱ㩛ב㓼㜔δѰ㣧㊿㋈ਇ㛨ᨆ 䳺ろⲺ⭕䮵ਇ㛨ခӄ㩛ޱ⭕䮵Ⲻ V9 䱬⇫θഖ↚൞ MON 87427 ⭕䮵ਇ㛨Ⲻ V8-V13 䱬⇫њ⅗௭᯳㥿⭎㟜䲚㥿ࡸਥԛᵶ↱㣧㊿∃㓼㜔θӰᐛࡑ䙖䳺ᙝу㛨Ⱦ ⭞ᴿ MON 87427 䖢ौ։Ⲻ㠠Ӛ㌱֒∃ᵢθ⭞ᴿެᆹᣍ㥿⭎㟜䖢ौ։Ⲻ㠠 ૂ〃Ӛ㌱֒⡬ᵢθਥԛᶺᡆӰᐛу㛨㌱〃㌱㔕Ⱦ⭞ MON 87427 䘑㺂⦿㊩ 〃ᆆ⭕ӝⲺᝅѿ൞ӄφㅢжθ໔ࣖᵸӚ〃〃Ⲻ⚫⍱ᙝȾ∃ᵢৱ䳺Ⲻީ䭤ᰬᵕᱥ Ԅ䳺ろᣳ࠰ࡦᮙ㊿䘏⇫ᰬᵕθᒩൽѰ 3-4 ཟȾᖉࢃӰᐛৱ䳺Ⲻᯯ⌋ऻᤢᵰỦᣳ䳺 δж㡢㾷 2 ⅗εૂᢁᐛᣳ䳺δж㡢 3 ⅗εȾу䇰ଠ〃ᯯ⌋θ䜳䴶㾷൞ 3-4 ཟⲺᰬ 䰪䠂䳼ѣ䏩ཕⲺӰ࣑ૂ䇴༽Ⱦ㙂䟽⭞ MON 87427 〃θਠ䴶㾷൞ V8-V13δཝ㓜 14 ཟεⲺᰬ䰪௭⍈њ⅗㥿⭎㟜䲚㥿ࡸȾഖ↚θ⦿㊩〃Ⲻ⚫⍱ᙝᴪ儎Ⱦㅢӂθ ࠅቇᵸӚ〃〃ⲺᡆᵢȾ䳅㔅⎄ਇኋθ䏀ᶛ䏀ཐߒѐӰਙ䖢ੇคᐸθᒬъࣩࣞ ࣑ᡆᵢⵕ൞уᯣрॽθ䟽⭞Ӱᐛৱ䳺Ⲻᯯ⌋ሼՐ䶘Ѫ⭞ᐛ⸣㕰ૂᵸӚ〃〃ᆆᡆᵢ ሯ䖹儎Ⲻ᥇ᡎȾ㙂䟽⭞ MON 87427 〃㌱㔕Րᴿࣟӄ䀙ߩ䘏ӑ䰤从Ⱦ пȽ։⭕⢟ᆜ⢯ᙝ MON 87427 Ⲻ䖢ौ։ᱥѠᵸӚ〃θ㓺ਾѰ LH198 × HiIIȾLH198 ޭᴿ㢥 Ⲻߒ㢰ᙝ⣬χ㙂 HiII ሯߒᵼ㨂ḉ∊䖹ᮅθᒬъᇯ᱉߃⭕Ⱦ։ኔӄṳฯླ ⦿㊩θѰжᒪ⭕֒⢟Ⱦ൞࠼㊱ᆜрኔӄঋᆆਬὃ⢟㓨Ƚ⿴ᵢ〇δGramineaeεȽ⦿ 㴶唃ᰅδMaydeaeεȽ⦿㴶唃ኔδZea L.εȽ⦿㊩〃δZea mays LεȽṳฯ⦿㊩Ӑ〃 δmaysεȾ⦿㊩ᱥᴿᙝ㑷⇌֒⢟θᱥ㠠ӚȽᵸӚൽӨਾⲺ⢟〃Ⱦ㣧㊿ⲺՖᯯᕅ ѱ㾷ᱥ伄Ֆ㊿θ㲡Ֆ㊿䖹ቇȾ⦿㊩ૂໞ㾵ଛ⦿㴶唃ޭᴿ䚍ՖਥӨਾᙝȾռໞ 㾵ଛ⦿㴶唃Ⲻ㠠❬࠼ᐹӻቶ䲆ӄ⋵ໞ㾵ଛૂধ൦傢㾵䜞Ⱦ⦿㊩ૂᬜ⿴ኔਥ ᵸӚθռᶷѰу᱉ȾᒬъᵸӚ〃ޭᴿ儎ᓜⲺу㛨ᙝθ൞䚍ՖᆜрҕуどᇐȾ ࠖⲴᒪᶛθ⦿㊩жᱥࣞ⢟侨ᯏૂӰ㊱Ⲻѱ伕Ⱦ⦿㊩㊳㋈ѣу∈㍖ૂ㠪 ᮅ㴁ⲳθ伕⭞⦿㊩ᰬਇ⭕Ⲻ䗽ᮅਃᓊᶷѰቇ㿷Ⱦռ⦿㊩ᴿࠖѠӰԢ⟕⸛Ⲻᣍ㩛 ޱഖᆆθऻᤢὃ䞮ȽзᐹȽỿᆆ㌌Ƚ㜦㴁ⲳ䞬ᣇࡸૂ㜦ࠓҩ㴁ⲳ䞬ᣇࡸȾ ታ㇗⦿㊩൞ޞь⮂ᒵ⌑〃ὃθռᱥӰԢᒬу䇚ѰᆹՐᡆѰ≮ѻᙝᵸ㥿ᡌ㘻 䳴ӄ䱨⋱Ⲻᵸ㥿Ⱦ↙ྸᡇԢԀཟᡶ⸛θ⭧ӄ⦿㊩䴂ᙝ㣧ᓅδ᷒ろε㜳䲆〃ᆆⲺ Ֆθഖ↚⦿㊩у㜳൞㠠❬⧥ູ䠂ᆎ⍱ Ƚะഖᬃ֒ MON 87427 ᱥ䙐䗽ߒᵼ㨂ԁሲ䖢ौ⦿㊩ᒲ㜐θሼ PV-ZMAP1043 䍞㋈ѣⲺ T-DNA ሲޛࡦ LH198 × HiII ։㌱ѣ㧭ᗍⲺȾT-DNA ऻжѠ⭧ e35S ࣞᆆȽ hsp70 ⲺᆆȽCTP2 ਬ㔵։䖢〱㛳ᓅࡍȽcp4 epsps ะഖ㕌⸷ᓅࡍૂ nos 㓾↘ ᆆᶺᡆⲺะഖ㺞䗴ⴈȾ Southern ᵸӚ㔉᷒㺞᱄θMON 87427 ਠᱥ൞ঋжփ⛯рᴿঋѠ䍓Ⲻ T-DNA ᨈޛχᨈޛⲺ cp4 epsps ะഖ㺞䗴ⴈ൞㔉ᶺрᱥᇂ᮪ૂᴿᓅⲺχ䍞㋈Ⲻ僞 ᷬᓅࡍ⋗ᴿᨈޛࡦ⦿㊩ะഖ㓺ѣȾ 176 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⭞ PCR ᯯ⌋ᢟ໔Ҽ 3 Ѡӈ䠃ਖⲺ DNA ⡽⇫θ䘏 3 Ѡ⡽⇫⏫ⴌҼޞ䮵 㘲ะഖ㓺ᓅࡍȾሼ䘏 3 Ѡᢟ໔ӝ⢟䘑㺂ᓅࡍ࠼᷆ᒬᤲθਥ⺤ᇐםᓅࡍૂޛᨈ ᓅࡍཝቅѰ 3746 bpθެѣޛ㘲ᓅࡍȾMON 87427 Ⲻᨈםᓅࡍะഖ㓺ޛ䮵ᨈޞ T-DNA ᨈޛѰ 3681 bpȾਜཌ൞ T-DNA Ⲻ 5'ㄥૂ 3'ㄥ࠼ࡡᴿ 41 bp ૂ 24 bp Ⲻᨈ փ⛯䘑ޛགྷᵰᕋ䎭Ⱦ䪾ሯᨈؤ䗽ぁѣਂ䬴ᯣ㻸ޛθ䘏ਥ㜳ᱥ⭧ӄ T-DNA ᨈޛ 㺂Ⲻ PCR ࠼᷆㔉᷒㺞᱄θ൞ T-DNA ᨈޛᰬ։ะഖ㓺 DNA ᴿ 140 Ѡ⻧ะ㻡ࡖ 䲚Ⱦռ䘏⇫ࡖ䲚ᓅࡍуޭᴿ⢯ᇐⲺ⭕⢟ᆜࣕ㜳Ⱦ ELISA ࠼᷆㔉᷒㺞᱄θMON 87427 Ⲻуੂ㓺㓽ૂಞᇎѣ CP4 EPSPS 㴁ⲳⲺ μg/g dwtȾެ 940 ٲքӄỶ࠰䲆δLODεࡦᴶཝٲ㺞䗴䠅ौ㤹പ䖹ཝθԄᴶቅ ѣਬ⡽ѣⲺᒩൽ㺞䗴䠅ᴶ儎δ㤹പԄ OSL3 Ⲻ 290 μg/g dwt ࡦ OSL1 Ⲻ δ㤹പԄ OSWP4 Ⲻ 240 μg/g dwt ࡦ OSWP1ٲμg/g dwtεχެ⅗ᱥ᮪Ṡᒩൽ 680 Ѱṯ㌱δԄ OSR3 Ⲻ 73 μg/g dwt ࡦ OSR1 Ⲻ⅗דⲺ 500 μg/g dwtεȾԛс 140 μg/g dwtεȽ൦р䜞᮪Ṡδ120 μg/g dwtεȽ㣧ѓδ100 μg/g dwtεȽ≊⭕ṯ δ72 μg/g dwtεȽ㺦㘷ṯ㌱δ72 μg/g dwtεȽ㥄ਬδ43 μg/g dwtεૂ㊳㋈δ4.2 μg/g dwtεȾ ᴿ 6 㣧㊿ṭδᶛ㠠 3 Ѡ䈋僂⛯εⲺ CP4 EPSPS 㴁ⲳ㺞䗴䠅քӄỶ࠰䲆δ 178 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ⓠф։Ⲻ䐓⿱䎻䗽 200 mθਇ⭕ะഖ┸〱Ⲻਥ㜳ᙝቧՐ䲃ࡦ 1%ԛсȾ⦿㊩ૂ жᒪ⭕ໞ㾵ଛ㊱㴶唃δZea mays subsp. mexicanaεໞ㾵ଛૂধ൦傢Ⲻжӑ൦॰ ྸ᷒〃ὃ䐓⿱ᖾ䘇θਥਇ⭕㠠❬ӈᦾ㊿ᒬ㺞⧦ᵸӚӨૂᙝȾռᴿ⹊ガ㺞᱄θ ሼ⦿㊩ૂໞ㾵ଛ⦿㴶唃〃൞ж䎭θӂ㘻ҁ䰪ਇ⭕ᔸӚⲺ仇⦽䘎ᱥᖾքⲺȾྸֵ᷒ 㘹㲇ᔶ㣧ᰬᵕȽ൦⨼䐓⿱䳊⿱ૂਇ㛨ぁᓜㅿഖ㍖θӂ㘻уའਥ㜳ਇ⭕㠠❬ᔸӚȾ ㊿㊩фᬜ⿴ኔδTripsacumεⲺཐᒪ⭕⢟〃ⲺᵸӚ䶔ᑮദ䳴Ⱦ↚ཌθֵᦾ⦿ ᵸӚᡆࣕθެԙҕՐу㛨ᒬъ൞䚍ՖрҕᱥуどᇐⲺȾ㠩ӄ MON 87427 ੇެ ᆹ⦿㊩ਇ⭕ะഖ┸〱уՐᕋ䎭ᆿޞᯯ䶘Ⲻ亴㲇θഖѰཝ䠅⹊ガᐨ㔅䇷᱄ ᑜᶛধᇩȾޞᓭૂ⧥ູᆿڛ㊱MON 87427 ऻⲺ cp4 epsps ะഖуՐሯӰ йȽ伕⭞ᆿޞ䇺ԭ 㩛ޱᆜ䇺ԭφԄ 2008 ᒪ㗄ള 3 Ѡ൦⛯Ⲻ⭦䰪䈋僂ѣ䟽䳼ṭ䘑㺂㩛ޱᡆ࠼ ࠼᷆Ⱦ࠼᷆Ⲻᡆ࠼ऻᤢ㩛ޱᡆ࠼Ƚᣍ㩛ޱᡆ࠼ૂ⅗⭕ԙ䉘⢟䍞Ⱦ㩛ޱᡆ࠼ऻᤢφ ㊳㋈ѣⲺᑮ㿺ᡆ࠼δ⚦࠼Ƚᣎ㇍Ⲻ⻩≪ौਾ⢟Ƚ≪࠼Ƚ㴁ⲳ䍞ૂ㜸㛠εȽ䞮ᙝ⍍ ⏚㓚㔪δADFεȽѣᙝ⍍⏚㓚㔪δNDFεȽᙱ㟩伕㓚㔪δTDFεȽ≞ะ䞮Ƚ㜸㛠䞮 δC8~C22εȽ⸵⢟䍞δ䫏Ƚ䬒Ƚ䫷Ƚ䭷Ƚ䭦Ƚ⼭Ƚ䫴Ƚ䫖ૂ䭂εૂ㔪⭕㍖δਬ䞮Ƚ ✕䞮Ƚ㔪⭕㍖ Aδ!㜗㩓㍖εȽ㔪⭕㍖ B1Ƚ㔪⭕㍖ B2Ƚ㔪⭕㍖ B6 ૂ㔪⭕㍖ Eε ԛ൦р䜞ὃṠⲺᑮ㿺ᡆ࠼ȽADFȽNDFȽ䫏ૂ⼭Ⱦᣍ㩛ޱᡆ࠼ऻᤢὃ䞮ૂỿ㊳ ٲ㌌Ⱦ⅗⭕ԙ䉘ӝ⢟ऻᤢ㌖䟑Ƚ䱵内䞮ૂሯ-俏䉼䞮Ⱦ4 Ѡѐौ৸➝〃Ⲻᒩൽ ⭞ᶛ⺤ᇐḆ〃ᡆ࠼Ⲻ 99%ᇯ䇮॰䰪Ⱦ൞࠼᷆Ⲻ 78 〃ᡆ࠼ѣθᴿ 16 〃㊳㋈ᡆ࠼δ15 〃㩛ޱᡆ࠼ૂ 1 〃ᣍ㩛ޱᡆ࠼ε䎻䗽 50%Ⲻ࠼᷆⋗ᴿ䗴ࡦᇐ䠅с䲆δLOQεθᰖ ⌋䘑㺂㔕䇗࠼᷆Ⱦഖ↚ਠሯެᆹᡆ࠼䘑㺂Ҽ㔕䇗࠼᷆Ⱦ൞䘑㺂㔕䇗࠼Ⲻᡆ࠼ѣθ MON 87427 ૂᑮ㿺ሯ➝൞ཝཐᮦᡆ࠼руᆎ൞㔕䇗ᆜᱴ㪍ᐤᔸȾቇ䠅ᆎ൞ᱴ㪍 㩳൞ѐौٲᐤᔸⲺᡆ࠼θ㾷Ѿᐤᔸ㺞⧦уж㠪θ㾷Ѿᐤᔸᖾቅθ㾷Ѿ࠼᷆ᒩൽ 〃Ⲻ 99%ᇯ䇮॰䰪ᡌ㘻㩳൞ ILSI ᮦᦤᓉᡌਇ㺞ᮽ⥤Ⲻ䈛ᡆ࠼ᮦᦤ॰䰪θ ᙱҁൽуޭᴿ⭕⢟ᆜᝅѿȾഖ↚θਥԛᗍ࠰㔉䇰䇚Ѱ MON 87427 ૂᑮ㿺ሯ➝൞ 㩛ޱᡆ࠼рᇔ䍞ㅿੂȾཟ⍛⯴⯻人䱨ѣᗹሯ MON 87427 ㊳㋈䘑㺂Ⲻᣍ㩛 ޱഖᆆᡆ࠼Ỷ⎁㔉᷒ҕ㺞᱄θ䖢 cp4 epsps ะഖᣍ䲚㥿ࡸ⦿㊩ MON 87427 ѣᣍ㩛 ޱഖᆆὃ䞮䠅Ѱ 20.3 mg/gθ䶔䖢ะഖᑮ㿺⦿㊩ EXP262 ѣὃ䞮䠅Ѱ 20.2 mg/gθ њ㘻⋗ᴿᱴ㪍ᐤᔸȾ ∈⨼ᆜ䇺ԭφCP4 EPSPS 㴁ⲳᶛⓆӄൕ༚ߒᵼ㨂θᱥ㠠❬⮂ѣὃ⢟ૂᗤ⭕ ⢟Ფ䚃ޭᴿⲺ EPSPS 㴁ⲳⲺж〃θޭᴿ䮵ᵕᆿޞᓊ⭞়ਨȾ㴁ⲳ⨼ौ⢯ᖷ⢯ᙝ ⹊ガ㔉᷒㺞᱄θMON 87427 ᶛⓆⲺ CP4 EPSPS 㴁ⲳфཝ㛖ᵼ㨂ᶛⓆⲺㅿੂȾ⭕ ૂ ؗᚥᆜ࠼᷆㔉᷒㺞᱄θMON 87427 㺞䗴Ⲻ CP4 EPSPS 㴁ⲳф TOX_2016⢟ PRT_2016 ᮦᦤᓉѣᐨ⸛Ⲻ∈㍖㴁ⲳૂᣍ㩛ޱഖᆆ⋗ᴿᓅࡍૂ㔉ᶺрⲺղᙝȾ ሯ 10 ਠ䳺ᙝૂ 10 ਠ䴂ᙝ CD-1 ቅ啖䘑㺂 CP4 EPSPS 㴁ⲳ⚂㛹θ䟽⭞ 3 Ѡࡸ䠅δ࠼ ࡡѰ 49Ƚ154 ૂ 572 mg/kg bwtε༺⨼Ⱦグⲳሯ➝㓺⭞ 50 mM ⻩䞮≘䫖㕉ߨ⏨༺ ⨼䳺ᙝૂ䴂ᙝቅ啖 10 ਠθࡸ䠅Ѱ 33.33 ml/kg bwtȾ䱪ᙝሯ➝㓺⭞⢑㹶ⲳ㴁 ⲳδBSAε༺⨼ 10 ਠ䳺ᙝૂ 10 ਠ䴂ᙝ CD-1 ቅ啖θ⚂㛹ࡸ䠅Ѱ 363 mg/kg bwtȾ ⎁ᓭ⣬߫Ⱦ∅ઞ䘑㺂ж⅗䈜㓼ⲺѪᓀ㿸ሕᒬڛૂ⦽ᇔ僂ᵕ䰪θ∅ཟњ⅗䈹ḛ↱ӗ ᇐ։䠃ૂ䘑伕䠅Ⱦ⭧ӄ⋗ᴿ㿸ሕࡦу㢥ਃᓊθㅢ 8 ཟ㔉ᶕ䈋僂θሼᡶᴿቅ啖䀙ࢌ 䘑㺂⯻⨼ᆜ㿸ሕȾᇔ僂ѣ⋗ᴿਇ⧦ CP4 EPSPS 㴁ⲳ༺⨼ሯӄቅ啖Ⲻᆎ⍱⦽ȽѪ 179 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᓀ㺞⧦Ƚ໔䠃Ƚ侨ᯏ੮᭬⭞ᡌᙱⲺ⯻⨼ᆜ㿸ሕᴿӶѾуᖧଃθཝ։䀙ࢌҕᵠ ਇ⧦㝅ಞᔸᑮȾCP4 EPSPS 㴁ⲳԛᇔ僂ѣⲺᴶ儎ࡸ䠅 572 mg/kg bwt ⚂㛹ᰬθ⋗ ᴿሯቅ啖ӝ⭕ԱуᖧଃȾഖ↚θCP4 EPSPS Ⲻᰖ᱄ᱴᦕᇩ֒⭞ࡸ䠅δNOAELε 㠩ቇѰ 572 mg/kg bwtȾṯᦤ↚ NOAEL ԛѣളӰ㗚㩛ޱ᩺ޛ≪ᒩ䈹ḛᮦᦤθ ਥԛ䇗㇍࠰䙐䗽㟩伕᩺ޛ MON 87427 ѣ CP4 EPSPS 㴁ⲳⲺᳪ䵨䲆δMOEε儎 䗴 1.4 × 104~7.9 × 105Ⱦഖ↚θMON 87427 ѣⲺ CP4 EPSPS 㴁ⲳуՐሯӰ㊱ૂࣞ ⢟ӝ⭕∈ᙝȾ 2016 ᒪθ⭧ӄߒѐ䜞䇺ᇗщᇬཐ⅗ሯ CP4 EPSPS 㴁ⲳቅ啖ᙛᙝ∈ᙝ⹊ガᵠ ћṲ䚫ᗠߒѐ䜞ީӄᙛᙝ∈ᙝ䇺ԭᯯ⌋㺞⽰亴㲇θᆕኧ䜳ޢਮ߃⅗ሯ CP4 EPSPS 㴁ⲳ䘑㺂Ҽቅ啖㔅ਙ⚂㛹ᙛᙝ∈ᙝ䈋僂Ⱦ⭞ཝ㛖ᵼ㨂㺞䗴㓥ौⲺ CP4 EPSPS 㴁ⲳԛ㔅ਙᯯᕅԛ 5000 mg/kg ։䠃ࡸ䠅ঋᰛњ⅗⚂㛹䴂䳺 10 ਠ CD-1 ቅ啖Ⱦ ੂᰬ䘑㺂䈋僂Ⲻ䘎ᴿ⓬ࡸሯ➝㓺ԛԛੂㅿࡸ䠅ቅ⢑㹶㴁ⲳδBSAε⚂㛹Ⲻ㴁 ⲳሯ➝㓺Ⱦᡶᴿࣞ⢟൞⚂㛹ࢃൽ⾷伕༺⨼θ⚂㛹∅ཟњ⅗㿸ሕቅ啖ᱥੜ↱ӗᡌ ᱥ㺞⧦࠰ѣ∈⯽⣬θ㿸ሕᵕѰ 14 ཟȾㅢ 14 ཟᰬሼᡶᴿࣞ⢟༺↱θ䘑㺂ཝ։䀙ࢌȾ ⹊ガ㔉᷒㺞᱄θᰖ䇰Ԅቅ啖ᆎ⍱⦽ȽѪᓀ㺞⧦Ƚ։䠃Ƚ։䠃໔ࣖȽ伕⢟⎾㙍ᡌཝ ։⯻⨼ᆜᯯ䶘ⵁθൽᵠਇ⧦ф༺⨼ީⲺу㢥ਃᓊȾഖ↚θ䙐䗽䈛ቅ啖ᙛᙝ∈ᙝ ⹊ガᗍࡦⲺ CP4 EPSPS 㴁ⲳⲺᰖ᱄ᱴᦕᇩ֒⭞ࡸ䠅δNOAELε㠩ቇѰ⹊ガᴶ儎 ࡸ䠅 5000 mg/kg bwθ⭧↚զ㇍ᗍࡦ䙐䗽㟩伕᩺ޛ MON 87427 ѣ CP4 EPSPS 㴁 ⲳⲺᳪ䵨䲆δMOEε儎䗴 6.9 × 106Ⱦ ߒѐ䜞ညᢎθཟ⍛⯴⯻人䱨ѣᗹሯ MON 87427 䘑㺂Ҽ伕⭞ᆿޞỶ⎁Ⱦ ሼ䖢 cp4 epsps ะഖᣍ䲚㥿ࡸ⦿㊩ MON 87427 ૂ䶔䖢ะഖӨᵢ⦿㊩ EXP262 ࠼ࡡ 侨ᯏδ∊ׁѰ 50% Ƚ25% Ƚ12.5%εஸ侨ཝ啖 90 ཟθஸ侨ᵕ䰪ࣞ⢟⍱ࣞ㠠ޛ᧰ θ㻡∑ᴿݿ⌳θ啱ȽȽਙ㞊ᰖᔸᑮ࠼⌂⢟Ⱦ㔕䇗㔉᷒㺞᱄䜞࠼ᑮ㿺ሯ➝䖢ྸ ะഖ䈋⢟㓺ࣞ⢟䘮伕䠅фᑮ㿺ะ侨ᯏሯ➝㓺∊䖹θᴿ㔕䇗ᆜᝅѿδP<0.05εθ ռൽᰖࡸ䠅ਃᓊީ㌱θᵠ㿷ެ⭕⢟ᆜᝅѿȾެ֏㓺ࣞ⢟։䠃Ƚ䘑伕䠅Ƚᙱ໔䠃Ƚ ᙱ䘑伕䠅ૂᙱ伕⢟⭞⦽фᑮ㿺ะ侨ᯏሯ➝㓺∊䖹θᐤᔸൽᰖ㔕䇗ᆜᝅѿ δP>0.05εȾ ᇔ僂ѣᵕૂᇔ僂ᵡᵕ亯㹶⏨ᆜḽδ㓘㓼㜔䇗ᮦȽ㹶㓘㴁ⲳȽ㹶ቅᶵȽ ⲳ㓼㜔䇗ᮦ࠼㊱εȽ㹶⭕ौḽδ㹶я≞䞮≞ะ䖢〱䞬Ƚཟ䰞ߢ≞䞮≞ะ䖢 〱䞬Ƚት㍖≤Ƚ㛼ര䞽Ƚ⭎⋯п䞥Ƚ㹶㌌Ƚҩ䞮㝧≘䞬Ƚᙱ㴁ⲳȽⲳ㴁ⲳȽ㛂䞆Ƚ 㤹പȾࣞ⢟䀙ࢌᰬθཝ։㿸ሕᵠਇ⧦ᔸᑮȾٲᙝ⼭䞮䞬εൽ൞ᵢỶ⎁ঋփ↙ᑮ⻧ 䳺ᙝࣞ⢟Өѣ㓺㛴/։∊քӄሯ➝㓺θӨ儎㓺㛓/։∊儎ӄሯ➝㓺θ䖢ք㓺㛓/։∊ 儎ӄሯ➝㓺θ䖢ք㓺ᗹ/։∊儎ӄӨք㓺θ䖢ѣ㓺㛴р㞰/։∊儎ӄӨѣ㓺θᐤᔸ ᴿ㔕䇗ᆜᝅѿδP<0.05εȾ䴂ᙝࣞ⢟Ө儎㓺㜮㞰/։∊Ƚ㛴/։∊儎ӄሯ➝㓺θ䖢 ѣ㓺㛓/։儎ӄሯ➝㓺θ䖢儎㓺㜮㞰/։∊Ƚ㛴/։∊儎ӄሯ➝㓺θ䖢ѣ㓺㛓/։儎ӄ Өѣ㓺θᐤᔸᴿ㔕䇗ᆜᝅѿδP<0.05εȾռр䘦ᐤᔸᰖࡸ䠅ਃᓊީ㌱θᵠ㿷ެ⭕ фሯ➝㓺∊䖹θᐤᔸൽᰖ㔕䇗ᆜٲ∍ᆜᝅѿȾެ֏㓺ࣞ⢟㝅ಞ䠃䠅ૂ㝅/։⢟ ᝅѿδP<0.05εȾ⯻⨼㓺㓽ᆜỶḛᵠਇ⧦䈛䖢ะഖṭᕋ䎭ᇔ僂ࣞ⢟ޮශ⯻⨼㓺 㓽ᆜ᭯ૂ⢯ᔸᙝᦕ՚Ⱦ 㠪ᮅᙝ䇺ԭφMON 87427 ㊳㋈ѣ㺞䗴Ⲻ CP4 EPSPS 㴁ⲳᒩൽ≪ᒩᱥ θ㙂㊳㋈ѣᒩൽᙱ㴁ⲳ䠅Ⲵ࠼ᮦѰ㊳㋈ᒨ䠃Ⲻ 10.05αδᡌ㘻䈪 180 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 100500 μg/g dwtεȾ䇗㇍ᗍࡦ CP4 EPSPS 㴁ⲳখ㊳㋈ᙱ㴁ⲳⲺ䠅Ѱ 0.004αθ ҕቧᱥখ㊳㋈ᙱ㴁ⲳⲺ∊ׁᖾቅȾ⭕⢟ؗᚥᆜ࠼᷆㔉᷒㺞᱄θMON 87427 㺞䗴 Ⲻ CP4 EPSPS 㴁ⲳф AD_2016 ᮦᦤᓉѣᐨ⸛Ⲻ㠪ᮅ㴁ⲳԛެᆹ⍱ᙝ㴁ⲳ⋗ᴿ ᓅࡍૂ㔉ᶺрⲺղᙝȾ⁗ᤕ⎾ौᇔ僂㔉᷒㺞᱄θཝ㛖ᵼ㨂ӝ⭕Ⲻ CP4 EPSPS 㴁ⲳ SGF 䠂䗻䙕㻡⎾ौȾṯᦤ SDS-PAGE ࠓ㜬ḉ㢨ૂ Western ࠼᷆㔉᷒θ࠼ࡡ ᴿ 98αૂ 95%ԛрⲺ CP4 EPSPS 㴁ⲳ൞ 15 s 㻡⎾ौȾੂṭθCP4 EPSPS 㴁ⲳ ൞ SGF 䠂ฯޱуࡦ 15 sθެ⍱ᙝ䲃քࡦ 10αԛсȾCP4 EPSPS 㴁ⲳⲺ✣どᇐᙝ ᇔ僂㔉᷒㺞᱄θᖉԛ 25ºCȽ37ºC ૂ 55ºC ࣖ✣ 15 min 㿸ሕࡦ CP4 EPSPS ⍱ᙝ ぃᴿ䲃քθ࠼ࡡ䲃㠩ሯ➝Ⲻ 81%Ƚ84%ૂ 70%χռࣖ✣ 30 min θ25ºC ༺⨼Ⲻ CP4 EPSPS ⍱ᙝ⋗ᴿौθ37ºC ༺⨼㓺Ⲻ䞬⍱䲃㠩ሯ➝ⲺⲺ 88%θ㙂 55ºC ༺⨼ 㓺Ⲻ CP4 EPSPS 㺞⧦Ѱཝᑻс䲃θӻѰሯ➝Ⲻ 25%Ⱦу䇰ᱥ༺⨼ 15 min 䘎ᱥ༺ ⨼ 30 minθ75°C ૂ 95°C ᓜᶗԬсⲺ CP4 EPSPS 䞬⍱ൽքӄỶ⎁䲆ȾSDS-PAGE ࠼᷆㔉᷒㺞᱄θ൞ᡶᴿ༺⨼ᰬ䰪ૂ༺⨼ᓜᶗԬсθṭⲺ 43.8 kDa ᶗᑜؗਭ ᕰᓜ⋗ᴿ᱄ᱴⲺ䲃քθ㺞᱄ࣖ✣༺⨼ӻՐֵ CP4 EPSPS 㴁ⲳⲺ䞬⍱ᙝ䲃քθռ уՐֵ CP4 EPSPS 㴁ⲳ䲃䀙Ⱦ㔲рᡶ䘦θCP4 EPSPS 㴁ⲳуՐᕋ䎭ᴪཐⲺ㠪ᮅ ᯯ䶘Ⲻ亴㲇Ⱦ ᣍ⭕㍖ᣍᙝφMON 87427 уԱᣍ⭕㍖ᣍᙝḽ䇦ะഖȾ ޡȽ䶔人ᵕ᭾ᓊ MON 87427 ѣሲޛⲺะഖᱥᶛⓆӄൕ༚ߒᵼ㨂 CP4 㨂ṠⲺ cp4 epsps ะഖθ ਥԛ㺞䗴 CP4 EPSPS 㴁ⲳȾEPSPS ൞ὃ⢟ૂᗤ⭕⢟ѣᲤ䚃ᆎ൞θᱥ㣩俏ᰅ≞ะ 䞮ਾᡆ䙊ᖺрⲺީ䭤䞬θެ֒⭞ᵰᖾᾐȾCP4 EPSPS ᱥ EPSPS 㴁ⲳᇬᰅѣ Ⲻжθфὃ⢟Ⓠ EPSPS ᴿᖾ儎ੂⓆᙝθ֒⭞ᵰੂθռޭᴿф㥿⭎㟜у ӨૂⲺ⢯ᙝȾഖ↚θԄሲޛཌⓆะഖ䀈ᓜᶛ䈪θਥԛ人ᵕ MON 87427 ᕋޛⲺ᯦ ᙝ⣬уՐᑜᶛ䶔人ᵕ᭾ᓊȾ ࠼ᆆ⢯ᖷ⢯ᙝ࠼᷆Ƚ㺞ශᙝ⣬ૂߒ㢰ᙝ⣬࠼᷆ԛ㓺ᡆᡆ࠼࠼᷆Ⲻ㔉᷒ҕ 㺞᱄θMON 87427 ᒬ⋗ᴿഖѰሲޛ CP4 EPSPS 㴁ⲳ㙂ᑜᶛ䶔人ᵕ᭾ᓊȾ ғȽ⭕ӝᓊ⭞ࢃᲥᤕ䟽Ⲻᆿޞⴇ᧠᯳ MON 87427 ሼ⭞ӄᶺᔰ᯦Ⲻ⦿㊩ᵸӚ〃〃㌱㔕ȾᴶᰟՐ൞㗄ളૂࣖᤵཝ ᔶခѐौᓊ⭞θਥԛ人ᵕሼᶛՐᲤ䚃ᆎ൞ӄ⦿㊩ᵸӚ〃ѣȾռᵢ⭩䈭ӻ䲆ӄ 䇷ҜȾ֒Ѱ MON 87427 Ⲻᢶᵥ⹊ਇθᆕޞ൞ѣള㧭ᗍ䘑ਙ⭞֒ࣖᐛᯏⲺᆿ ኧ䜳ޢਮՐ㾷≸䍮᱉ћṲ䟽᧠᯳θ䚫ᆾީ⌋ᗁ⌋㿺θऻ㻻䘆䗉㾷ਥ䶖θḽ ᢶᵥሲૂ᭥ᤷθԛؓ䇷ב䇼㾷ᾐθ䘆䗉⧥㢸ћṲᬃ֒θᗻ㾷ᰬੇ䍮᱉ᨆ MON 87427 Ⲻ䍮᱉ㅜਾߒѐ䜞亷ਇⲺᆿޞ䇷ҜⲺ㾷≸Ⱦ ॷȽ㔉䇰 ᴿ䍕䶘ᖧଃθ⋖ޞᓭૂ⧥ູᆿڛ㊱ཝ䠅Ⲻ⹊ガ㔉᷒㺞᱄θMON 87427 ሯӰ ф։ૂᑮ㿺⦿㊩ੂṭᆿޞȾ 181 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 9. 䈛㊱䖢ะഖὃ⢟ളཌ⭕ӝᓊ⭞Ᾰ߫ 2016 ᒤᱟ䖜สഐ⢙୶ъॆㅜ 21 ᒤDŽ൘ 2014 ᒤˈ࡙⭘⭏⢙ᢰᵟᔰਁⲴ䖜 สഐ⢙൘ޘ⨳ⲴἽ䶒〟䗮ࡠ 1.81 ӯޜ产ˈ∄ 2013 ᒤⲴ 1.752 ӯޜ产໎䮯Ҷ 3.6ˁ˗Ӿ 1996 ᒤⲴ 170 зޜ产ࡠ 2014 ᒤⲴ 1.815 ӯޜ产ˈޘ⨳䖜สഐ⢙Ⲵ Ἵ䶒〟໎䮯Ҷ 100 ཊؽˈ䘉а໎䮯֯ᗇ䖜สഐᢰᵟᡀѪ⧠ԓߌъਢкᓄ⭘ᴰѪ䗵 䙏Ⲵ⢙ᢰᵟDŽ 2013 ᒤ 10 ᴸ 30 ᰕˈ䲿⵰ 4 њ䖜สഐᣇ㲛㤴ᆀ૱㧧߶⭘Ҿᆀ⭏ӗ઼୶ ъॆἽˈᆏ࣐ഭᡀѪޘ⨳ㅜ 28 њἽ䖜สഐ⢙ⲴഭᇦDŽ㖾ഭǃᐤ㾯ǃ䱯 ṩᔧǃঠᓖǃ࣐བྷ઼ѝഭӽ❦ᱟᴰѫ㾱䖜สഐ⢙ἽഭᇦDŽޡᴹ 19 њ䖜ส ഐ⢙ἽབྷഭⲴἽ䶒〟൘ 5 зޜ产ԕкDŽᒦфˈ䲿⵰䎺ই઼ঠᓖቬ㾯ӊҾ 2014 ᒤ࠶࡛ᢩ߶Ҷ䖜สഐ⦹㊣઼⭈㭇Ⲵ୶ъॆἽˈᴹᵋ 2015 ᒤޘ⨳䖜สഐ ⢙Ἵഭᇦ䗮ࡠ 30 њDŽ ቡ䖜สഐᙗ⣦㘼䀰ˈӾ 1996 ᒤ䖜สഐ⢙俆⅑୶ъॆ㠣Ӻˈᣇ䲔㥹ࡲᙗ⣦ 㓸ᱟ䖜สഐ⢙Ⲵѫ㾱ᙗ⣦DŽ2014 ᒤˈᣇ䲔㥹ࡲⲴ䖜สഐ⢙˄ѫ㾱वᤜབྷ 䉶ǃ⦹㊣ǃ⋩㨌ǃỹ㣡ǃ⭌㨌઼㤌㬯˅Ἵ䶒〟Ѫ 1.02 ӯޜ产ˈঐޘ⨳䖜สഐ ⢙Ἵ䶒〟Ⲵ 57ˁDŽ༽ਸᙗ⣦ӗ૱ᱟ 2010 ᒤ̚2013 ᒤᵏ䰤໎䮯ᴰᘛⲴа㊫ᙗ ⣦ˈ2014 ᒤਜ਼ᴹєᡆԕкᙗ⣦Ⲵ༽ਸᙗ⣦䖜สഐ⢙Ἵ䶒〟䗮ࡠ 5140 зޜ 产ˈঐᙫ䖜สഐ⢙Ἵ䶒〟Ⲵ 28%ˈ∄ 2013 ᒤ໎࣐ 9%ˈᐢ㓿䘌䘌䎵ࠪҶঅ аⲴ Bt ᣇ㲛ᙗ⣦ӗ૱˄2014 ᒤἽ䶒〟 2740 зޜ产ˈঐ 15%˅DŽ2014 ᒤб 㖾⍢Ⲵᐤ㾯ǃ䱯ṩᔧǃᐤ൝઼Ѽ൝䱶㔝ᔰἽᣇ㲛㙀䲔㥹ࡲⲴ༽ਸᙗ⣦བྷ 䉶ˈᖃᒤἽ䶒〟ণ䗮 580 зޜ产DŽ༽ਸᙗ⣦ᰕ⳺ᡀѪ䖜สഐ⢙Ⲵањ䟽㾱䀂 㢢˄James, 2014˅DŽ 䖜สഐ⦹㊣ᱟӵ⅑Ҿབྷ䉶ⲴㅜҼབྷ䖜สഐ⢙ˈ2013 ᒤἽ䶒〟䎵䗷 5740 зޜ产ˈঐޘ⨳䖜สഐ⢙Ἵ䶒〟Ⲵ 33%DŽަѝˈᣇ䲔㥹ࡲ઼ᣇ㲛༽ਸᙗ⣦⦹ ㊣Ⲵ䶒〟ᴰབྷˈѪ 3730 зޜ产ˈঐޘ䜘䖜สഐ⢙ᓄ⭘䶒〟Ⲵ 23%˗অ㓟ᣇ䲔 㥹ࡲⲴ⦹㊣䶒〟Ѫ 770 зޜ产ˈঐ 5%˗অ㓟ᣇ㲛⦹㊣䶒〟Ѫ 600 зޜ产ˈঐ 4%DŽ 2013 ᒤˈޘ⨳ᴹ 17 њഭᇦᢩ߶୶ъॆἽ䖜สഐ⦹㊣ˈަѝᧂࡽ 5 սⲴഭᇦ 䖜สഐ⦹㊣Ἵ䶒〟䎵䗷 100 зޜ产ˈ࠶࡛Ѫ˖㖾ഭ˄3560 зޜ产˅ǃᐤ㾯˄1290 зޜ产˅ǃ䱯ṩᔧ˄320 з ޜ 产 ˅ǃই 䶎˄ 240 зޜ产˅઼࣐བྷ˄170 зޜ产˅DŽ ⅗ⴏⲴ 5 њᡀઈഭ൘ 2013 ᒤ㔗㔝 Bt 䖜สഐ⦹㊣ⲴἽˈᙫ䶒〟䗮ࡠ 148,013 ޜ 产ˈ∄ 2012 ᒤ໎䮯㓖 15%DŽ Klumper ઼ Qaim˄2014˅࡙⭘൘ц⭼ൠ䘋㹼Ⲵߌ൪䈳ḕᡆ⭠䰤䈅傼ᗇࠪ Ⲵᮠᦞሩ䗷৫ 20 ᒤޘ⨳ 147 亩ᐢ⸕Ⲵ䖜สഐ⢙⹄ウ䘋㹼Ҷѕ䉘Ⲵ㔬ਸ࠶ ᷀ˈᒦᣕҶ䖜สഐབྷ䉶ǃ⦹㊣઼ỹ㣡ሩ⢙ӗ䟿ǃߌ㦟֯⭘઼ߌ≁࡙⏖ᯩ䶒Ⲵ ᖡ૽DŽ䈕㔬ਸ࠶᷀Ⲵ㔃䇪ᱟĀ䖜สഐ⢙൘ 1995 ᒤ㠣 2014 ᒤ䰤ӗ⭏Ҷཊ䟽䟽བྷ ˈ᭸⳺˖䟷⭘䖜สഐᢰᵟ֯ॆᆖߌ㦟Ⲵ֯⭘⦷䱽վҶ 37ˁˈ⢙ӗ䟿ᨀ儈Ҷ 22ˁ ߌ≁࡙⏖໎࣐Ҷ 68ˁDŽᣇ㲛⢙Ⲵӗ䟿໎⳺઼ߌ㦟߿ቁⲴ〻ᓖབྷҾᣇ䲔㥹ࡲ ⢙DŽਁኅѝഭᇦ∄ਁ䗮ഭᇦⲴӗ䟿઼࡙⏖໎⳺儈DŽā䈕㔃䇪ঠ䇱ҶԕᖰⲴޘ⨳ᒤ ᓖ⹄ウᗇࠪⲴа㠤ᙗ㔃᷌˖Ӿ 1996 ᒤࡠ 2013 ᒤ䰤⢙ӗ䟿໎࣐Ⲵՠ㇇ԧ٬Ѫ 182 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ӯ㖾ݳˈަѝˈਁ䗮ഭᇦ㧧ᗇⲴ㍟䇑㓿⍾᭸⳺㓖Ѫ 652 ӯ㖾ݳˈਁኅѝഭ 1333 ᇦӗ⭏Ⲵ㓿⍾᭸⳺㓖Ѫ 681 ӯ㖾ݳ˄Brookes and Barfoot, 2015˅DŽ MON 87427 ᱟㅜањ㓴㓷⢩ᙗ㺘䗮ᣇ㥹⭈㟖Ⲵ⦹㊣ˈਟԕ㻛⭘ᶕ䇔Ѫࡋ䙐 䳴ᙗн㛢DŽഐ↔ਟԕ⭘Ҿ⦹㊣ᵲӔࡦDŽMON 87427 ᴰᰙҾ 2012 ᒤ൘࣐བྷ 㧧߶୶ъॆἽ䇨ਟˈ䲿ਾ൘㖾ഭҏ㧧ᗇҶἽᢩ߶DŽMON 87427 ሶՊ俆ݸ൘ 㖾ഭ઼࣐བྷ⭘Ҿ୶ъॆᵲӔࡦDŽ 䖜 cp4 epsps สഐ㙀䲔㥹ࡲ⦹㊣ MON 87427 ᴰᰙҾ 2012 ᒤ൘࣐བྷ㧧߶୶ ъॆἽ䇨ਟˈ䲿ਾ൘㖾ഭҏ㧧ᗇҶἽᢩ߶ˈᒦݸਾ൘໘㾯କǃ◣བྷ࡙ӊ/ᯠ 㾯ޠǃਠ⒮ǃᰕᵜǃ丙ഭǃକՖ∄ӊǃ㨢ᖻᇮǃ䎺ইǃ⅗ⴏㅹഭᇦ઼ൠ४㧧ᗇ䘋 ਓᢩ߶⭘Ҿ伏⭘઼侢⭘DŽާփᇑᢩᛵߥ䈧㿱л㺘ˈਾ䱴䘋ਓഭᢩ߶᮷Ԧ᮷৺㘫 䈁DŽӗൠഭᢩ߶᮷Ԧ᮷৺㘫䈁䈧㿱ㅜ 271 亥Ā12. 䖜สഐ⭏⢙൘䗃ࠪഭᇦᡆൠ ४ᐢ㓿ݱ䇨Ѫᓄ⭘䙄ᒦᣅ᭮ᐲ൪Ⲵ䇱᰾᮷ԦāDŽ 183 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䖜 cp4 epsps สഐ㙀䲔㥹ࡲ⦹㊣ MON 87427 ⴞࡽ൘ޘ⨳㤳തⲴᢩ߶ᛵߥ ൦॰ ᇗᵰᶺ ⭞䙊 ᒪԳ ࣖᤵཝ ࣖᤵཝড⭕䜞δHCε伕⭞2012 ᒪ ࣖᤵཝ ࣖᤵཝ伕Ỷ⯡ቶδCFIAε侨⭞/〃ὃ 2012 ᒪ 㗄ള 㗄ള伕ф㦥ⴇ㇗⨼ቶδFDAε 伕⭞/侨⭞ 2012 ᒪ 㗄ള 㗄ളߒѐ䜞ࣞὃ⢟Ỷ⯡ቶδUSDA- APHISε 〃ὃ 2013 ᒪ ໞ㾵ଛড⭕䜞δMOHε – ໞ㾵ଛ 伕⭞ 2012 ᒪ 㚊䛜ড⭕伄䲟人䱨㚊䛜ညՐδCOFEPRISε ◩ཝӐ/ ◩᯦伕ḽ㇗⨼ቶδFSANZε伕⭞2012 ᒪ ᯦㾵ަ ਦ⒴ ਦ⒴㺂᭵䲘ড⭕㖨δDOHε伕⭞2012 ᒪ ᰛᵢ ᰛᵢ⭕ࣩࣞⴷδMHLWε 伕⭞ 2013 ᒪ ᰛᵢ ᰛᵢߒ᷍≪ӝⴷδMAFFε 侨⭞ 2013 ᒪ ᰛᵢߒ᷍≪ӝⴷδMAFFε- ᰛᵢ ⧥ູ 2013 ᒪ ᰛᵢ⧥ؓ㖨δMOEε 丟ള 丟ളߒᶇਇኋ㇗⨼ቶδRDAε 侨⭞ 2013 ᒪ 丟ള 丟ള伕㦥㇗⨼ቶδMFDS) 伕⭞ 2014 ᒪ ଛ՜∊Ӑളᇬড⭕䜞δCTN-Healthε- ଛ՜∊Ӑ 伕⭞ 2013 ᒪ 伕㦥ⴇቶδINVIMAε ଛ՜∊Ӑളᇬ⭕⢟ᢶᵥညՐδCTNbioε- ଛ՜∊Ӑ 侨⭞ 2014 ᒪ ߒѐ⹊ガᡶδICAε 㨨ᗁᇴ 㨨ᗁᇴߒѐ䜞ὃ⢟ӝѐቶδBPIε 伕⭞/侨⭞ 2014 ᒪ 䏀 䏀ߒѐૂߒᶇਇኋ䜞δMARDε 伕⭞/侨⭞ 2015 ᒪ ⅝ⴕ ⅝ⴕညՐδECε 伕⭞/侨⭞ 2015 ᒪ ᯦ࣖ AVA 伕⭞/侨⭞ 2016 ᒪ 184 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 1Ƚໞ㾵ଛড⭕䜞ᮽԬ δMOHθ伕⭞ε 185 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ড⭕䜞 㚊䛜ড⭕伄䲟人䱨ညՐ ড⭕ညՐ 䖢ะഖ⭕⢟ѐौૂ䘑ਙⲺড⭕ 㕌ਭφ123300913X0002 ᦤφໞ㾵ଛਾՍള᭵⋱ᇠ⌋ㅢ 4 ᶗㅢ 3Ƚ8Ƚ14 ૂ 16 亯θ㚊䛜㺂᭵ぁᓅד ⌋Ṿㅢ 1Ƚ3Ƚ16-X 亯θໞ㾵ଛ䙐⭞ড⭕⌋ㅢ 3-XXIV ᶗȽㅢ 4-III-17 ᶗȽㅢ 4-IV ᶗȽㅢ 194-I-282Ƚ194-I-282-1Ƚ194-I-282-2 ᶗȽㅢ 368 ᶗȽㅢ 371 ᶗθড⭕ 䜞䜞⌋㿺ㅢ 2 ㄖㅢ c-X 㢸θ䖢ะഖ⭕⢟ᆿޞ⌋ㅢ 91Ƚ93Ƚ94 ૂ 97 ᶗθ2008 ᒪ 3 ᴾ 19 ᰛ㚊䛜ᇎᯯޢрਇᐹⲺ䖢ะഖ⭕⢟ᆿޞ⌋㿺ㅢ 23Ƚ24Ƚ25 ૂ 28 ᶗθ㚊䛜ড⭕伄䲟人䱨㇗⨼ညՐ⌋㿺ㅢ 1 ᶗȽㅢ 3-I-K ૂ 3-IȽ-VȽ-VIIȽ-XI ૂ-XII ᶗȽㅢ 4 ᶗȽㅢ 12-I ᶗૂㅢ 14-I ᶗθ2004 ᒪ 12 ᴾ 28 ᰛ㚊䛜ᇎᯯޢр ਇᐹⲺᇐ㚊䛜ড⭕伄䲟人䱨ညՐ㇗⨼ᵰᶺ㚂㜳ԙ⨼অ䇤θᒬ㘹㲇ࡦ伄䲟䇷ᦤ ᦤᡶ᭬ࡦⲺ⭩䈭䍺ᯏㆴਇⲺ䇺զᮽԬθ⢯㔏Ҿԛсӝԛφד㇗⨼ညՐ ᤷᴿӰφ Engineer Jose Javier Gándara Espinosa Monsanto Comercial S.A. de C.V. Prolongación Paseo de la Reforma No. 1015 Torre A Piso 21, Colonia Desarrollo Santa Fe Delegación Alvaro Obregón C.P 01376 Mexico D.F. 㚊䛜᭵ᓒ⌞߂ⲱ䇦ਭδR.F.C.ε MCO-701113-C5A 186 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ӝ ⢯ ᖷ ѐौ〦φ RHS ⦿㊩ ᆜφ Zea mays 〇 Familyφ ⿴ᵢ〇 Graminaceous ኔ Genreφ ⦿㴶唃ኔ Zea 〃 Speciesφ ⦿㊩〃 mays ṳฯ〃 Cultivarφ Zea mays OECD 䇼ࡡਭ⸷φ MON-87427-7 䖢ौ։㊱ශφ ߒᵼ㨂ԁሲ䖢ौ ࣕ㜳ᙝ⣬φ 㓺㓽⢯ᔸᙝᣍ㥿⭎㟜 䚍Ֆどᇐᙝ どᇐᙝ儎 ᇔ䍞ㅿੂᙝφ фੂශᑮ㿺ሯ➝〃ᇔ䍞ㅿੂ ᓊ⭞㤹പφ ࣞ⢟侨⭞ᡌӰ㊱伕⭞Ⲻ䘑ਙૂѐौ 㤹പφ ޞള॰ต ⣬ᘷφ ㊳㋈Ƚ㣧ࢥӝ 㠪ᮅᙝφ ք ∈ᙝφ ᰖ∈ ӝളφ 㗄ള 亯ⴤᶛⓆളᇬφ 㗄ള 㺞䗴㴁ⲳφ 㓺㓽⢯ᔸᙝ㺞䗴 CP4 EPSPS 㴁ⲳ 㿺ㅢ 37 ૂ 40⌊ޞṾㅢ 69 ૂ 98 ᶗȽ䖢ะഖ⭕⢟ᆿ⌊ޞᦤ䖢ะഖ⭕⢟ᆿד ᶗθؓ⮏㔝㔣ሯр䘦䘑㺂ᇗṮૂӝ䘑㺂䇺ԭⲺᵹȾ ᰛᵕφ2012 ᒪ 11 ᴾ 16 ᰛ ᛞ䈐ᥐⲺ FEDERICO ALBERTO ARGÜELLES TELLO ড⭕伄䲟人䱨ညՐщ 187 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 188 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 189 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2Ƚ◩ཝӐ-᯦㾵ަ伕ḽ㇗⨼ቶᮽԬ δFSANZθ伕⭞ε 190 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䇘ؤㅢ 133 ਭ ԛсޢൽѰɅ㚊䛜⌋ᮽҜޢᣛδFederal Register of Legislative 䇘ȾؤɆѣжᒬᖈѰㅢ 133 ਭޢθ൞Ʌ伕ḽޢInstrumentsεɆѣ⤢ ᇯࡍ㺞 伕ḽδ⭩䈭ਭφA1064——CV127 ᣍ䲚㥿ࡸཝ䉼ᶛⓆ伕εᴪ 伕ḽδ⭩䈭ਭφA1066——MON 87427 ᣍ䲚㥿ࡸ⦿㊩ᶛⓆ伕εᴪ 191 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 伕ḽδ⭩䈭ਭφA1066——MON 87427 ᣍ䲚㥿ࡸ⦿㊩ᶛⓆ伕εᴪ ᦤɅ◩᯦伕ḽ⌋Ṿδ1991εɆדཝӐ-᯦㾵ަ伕ḽ㇗⨼ቶ⨼ӁՐ◩ ㅢ 92 ㄖ㿺ᇐθㆴਇԛсᴪ䙐⸛Ⱦḽ⭕᭾ᰛᵕ䚫➝ᵢᴪޢѣᶗⅴ 3 ᇯȾ 2012 ᒪ 7 ᴾ 9 ᰛ ⌋ᗁф⌋㿺Ӂࣗ䜞θᙱ⨼Ӂ ԙ㺞◩ཝӐ-᯦㾵ަ伕ḽ㇗⨼ቶ⨼ӁՐ 1 〦 ᵢޢᱥሯ伕ḽδ⭩⭩䈭ਭφA1066——MON 87427 ᣍ䲚㥿ࡸ⦿㊩ᶛⓆ 伕εⲺᴪȾ 2 ሯɅ◩ཝӐ-᯦㾵ަ伕ḽ⌋ޮɆѣḽⲺᴪ Ʌ◩ཝӐ-᯦㾵ަ伕ḽ⌋ޮɆѣḽⲺᇯࡍ㺞ਇ⭕ᴪȾ 3 ⭕᭾ᰛᵕ 䈛ᴪ㠠 2012 ᒪ 7 ᴾ 12 ᰛ䎭⭕᭾Ⱦ ᇯࡍ㺞 [1] ḽ 1.5.2 ഖ᯦໔ྸсᓅࡍਭ㙂ਇ⭕ᴪφ 2.21 MON 87427 ᣍ䲚㥿ࡸ⦿㊩ᶛⓆ伕 δ䈇⌞φCV127 ѰެԌޢਮཝ䉼ӝθ㘱䈇⮛ε 192 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 193 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 194 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 195 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 196 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 197 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3Ƚਦ⒴㺂᭵䲘ড⭕㖨ᮽԬ δDOHθ伕⭞ε 198 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 199 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 200 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 4Ƚᰛᵢ⭕ࣩࣞⴷᮽԬ δMHLWθ伕⭞ε 201 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᰛᵢ⭕ࣩࣞⴷδMHLWεޢㅢ 162 ਭ 䢪ӄԛс⭞ DNA 䠃㓺ᢶᵥӝ⭕Ⲻ⭕⢟ᐨ㔅䙐䗽伕ф伕ࣖࡸㅢ 1A-2 ਭḽδ1959 ᒪ⭕ⴷޢㅢ 370 ਭεᇗḛぁᓅθཝ㠙↙ᕅᇙᐹԛс⭕⢟ᐨ㔅 ➝䠃㓺 DNA ᢶᵥᶛⓆⲺ伕ૂ伕ࣖࡸᆿޞ䇺ԭぁᓅㅢ 3Њ4 ਭ㿺ᇐδ2000 ᒪ MHLW ޢㅢ 233 ਭεθᇂᡆҼᆿޞᙝ䇺ԭȾ 2013 ᒪ 5 ᴾ 2 ᰛ Norihisa Tamura ⭕ࣩࣞⴷཝ㠙 䙐䗽Ҽ伕ૂ伕ࣖࡸ䇺ԭぁᓅⲺ䠃㓺 DNA ᢶᵥᶛⓆⲺ⭕⢟φ ӝ ㌱/䖢ौ։〦 ⭩䈭Ӱ/⢟֒ 㥿⭎㟜䈧ሲ䳺ᙝу㛨ૂ㙆㥿⭎㟜⦿ ⦿㊩ ᆕኧ䜳ᰛᵢᴿ䲆ޢਮ ㊩ MON 87427 202 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 203 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 5Ƚᰛᵢߒ᷍≪ӝⴷᮽԬ δMAFFθ侨⭞ε 204 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䠃㓺 DNA ᢶᵥᶛⓆⲺ侨ᯏᇂᡆᆿޞᙝ⺤䇚δᇎᯯޢε ᦤ侨ᯏૂ侨ᯏࣖࡸᡆ࠼ḽⲺ䜞㓝ᶗׁδ1976 ᒪߒ᷍≪ӝ䜞㓝ᶗד䢪ӄ ㅢ 35 ਭε䱺Ԭ 1-1-(1)Ⲻ㾷≸θߒ᷍≪ӝⴷδMAFFε⺤䇚Ҽԛс⭞䠃㓺 DNAׁ ᢶᵥ⭕ӝⲺ侨ᯏⲺ侨⭞ᆿޞᙝθᵢ䜞䮵ਇ㺞ᇎᯯޢφ➝䠃㓺 DNA ᢶᵥӝ⭕ Ⲻ侨ᯏૂ侨ᯏࣖࡸⲺᆿޞぁᓅδ2002 ᒪ 11 ᴾ 26 ᰛߒ᷍≪ӝⴷㅢ 1780 ਭޢ εㅢ 4 ⅴ㿺ᇐⲺ㾷≸θԛс侨⭞ᆿޞᙝᗍࡦ⺤䇚Ⱦ 2013 ᒪ 5 ᴾ 2 ᰛ Yoshimasa Hayashi ߒ᷍≪ӝⴷཝ㠙 ᇂᡆҼᆿޞᙝ⺤䇚Ⲻ䠃㓺 DNA ᢶᵥᶛⓆⲺ侨ᯏφ ӝ ㌱/䖢ौ։〦 ⭩䈭Ӱ/⢟֒ ਮޢ㊩ ᣍ䷎㗻ⴤᇩ㲡⦿㊩䖢ौ։ 5307 ݾ↙䗴ᰛᵢᴿ䲆⦿ 㥿⭎㟜䈧ሲ䳺ᙝу㛨ૂ㙆㥿⭎㟜⦿ ⦿㊩ ᆕኧ䜳ᰛᵢᴿ䲆ޢਮ ㊩ MON 87427 205 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 206 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 6Ƚᰛᵢߒ᷍≪ӝⴷ-ᰛᵢ⧥ؓ㖨ᮽԬ δMAFF / MOEθ⧥ູε 207 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᭬ԬӰφSeiichiro Yamane ᆕኧ䜳ᰛᵢᴿ䲆ޢਮθ⌋Ӱԙ㺞ᢝ㺂ᙱ㻷 ᇺԬӰφYoshimasa Hayashi ߒ᷍≪ӝⴷཝ㠙 Nobuteru Ishihara ⧥ູⴷཝ㠙 ഔགྷφሯ㊱ශ I ⭞䙊Ⲻ⌋㿺 ᦤ䙐䗽┗⍱ᙝ᭯䙖⭕⢟։ᓊ⭞ሯ⭕⢟ཐṭᙝ䘑㺂ؓᣚфਥᤷ㔣ᓊ⭞Ⲻ⌋ᗁד ਮṯᦤ䙐䗽㿺㤹⍱ᙝ᭯䙖⭕⢟։ޢߒ᷍≪ӝⴷཝ㠙ૂ⧥ູⴷཝ㠙䙐⸛ᛞθ֖ ᓊ⭞ሯ⭕⢟ཐṭᙝ䘑㺂ؓᣚфਥᤷ㔣ᓊ⭞⌋δ2003 ᒪㅢ 97 ਭ⌋ᗁεㅢ 4 ᶗㅢ 1 䜞࠼ӄ 2011 ᒪ 6 ᴾ 17 ᰛᨆӚⲺ⭩䈭θྸ䱺Ԭ㺞Ṳᡶࡍθᐨ㔅㧭ᗍ㊱ශ 1 ⭞䙊 δ1 亯εȾ 208 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 δ䱺㺞ε 㕌ਭ: 13-46P-0025 㧭ᗍޢਮθ⌋Ӱ ᆕኧ䜳ᰛᵢᴿ䲆ޢਮ ԙ㺞ဉфࣔޢᇚ൦ Seiichiro Yamaneθᙱ㻷 ൶ 4-10-10 Ginza, Chuo-ku, Tokyo ㊱ශ 1 ⭞䙊 侦Ⲻؤ⍱ᙝ᭯䙖⭕⢟։〦 㥿⭎㟜䈧ሲ䳺ᙝу㛨ૂ㙆㥿⭎㟜⦿㊩δ㔅䗽 cp4 epspsθZea mays subsp. mays (L.) IltisεδMON87427, OECD UIφMON-87427-7ε ⍱ᙝ᭯㢥⭕⢟։㊱ශ ᶛ㠠ӄ⍱ᙝ᭯㢥⭕⢟։Ⲻ伕ᡌ侨ᯏⲺᓊ⭞ȽṳฯȽࣖᐛȽ ⭞䙊Ⲻᇯ ۞ᆎȽ䖢〱ૂ༺⨼θԛެᆹީ㺂ѰȾ 1 ⍱ᙝ᭯㢥⭕⢟։㊱ශ _ 1 ⭞䙊ᯯᕅ 209 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 210 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 211 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 7Ƚ丟ളߒᶇਇኋ㇗⨼ቶᮽԬ δRDAθ侨⭞ε 212 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߒᶇਇኋ㇗⨼ቶ ᭬ঋփྸс 从ⴤφߒѐᓊ⭞⭕⢟ᢶᵥ᭯㢥⭕⢟ MON 87427 ⧥ູ伄䲟䇺ԭᇗḛ㔉᷒ޢ ᦤɅ⍱ᙝ᭯㢥⭕⢟։ㅿ䏀ູ䖢〱⌋Ɇㅢ 16 ᶗⅴδ伄䲟䇺զ䈘εדഔགྷφ .1 ૂㅢ 13 ᶗⅴδ伄䲟䇺զညᢎᇔ᯳⍷ぁεθԛɅ䪾ሯ⍱ᙝ᭯㢥⭕⢟։ㅿ 䏀ູ䖢〱Ⲻ㔕жޢɆㅢ 1-4 ᶗδԙ㺞/ㆴ㓜ᵰᶺεㅢж⇫ૂ 4-8 ᶗδ⧥ ູ伄䲟䇺զㅿεȾ ਮᨆӚⲺ⍱ᙝ䚍Ֆ᭯㢥⦿㊩ޢṯᦤԛрؗᚥθᡇԢ䙐䱺Ԭѣᆕኧ䜳丟ള .2 δMON 87427εߒѐᓊ⭞Ⲻᇗḛ㔉᷒Ⱦ a. ᇗḛ亯ⴤφ䚍Ֆ᭯㢥ᣍ䲚㥿ࡸδ㓺㓽䘿ᙝε⦿㊩δMON 87427ε b. ⭩䈭Ӱφᆕኧ䜳丟ളޢਮ c. ⭩䈭ᰛᵕφ2011 ᒪ 6 ᴾ 28 ᰛ 䱺Ԭφ䚍Ֆ᭯㢥⍱ᙝ⭕⢟։ߒѐᓊ⭞⧥ູ伄䲟ᇗḛ㔉᷒δNo 2013-07εȾ㔉ᶕȾ ߒᶇਇኋ㇗⨼ቶቶ䮵 ᭬Ӱφ伕ȽߒѐȽ᷍ѐૂ⑊ѐ䜞䜞䮵δḽ䇼ȽỶ⯡ૂⴇ༺༺䮵εȽളᇬߒ ѐӝ䍞䠅㇗⨼ᵃࣗ䜞δӝ൦Ỷ⎁ഘ䱕䍕䍙ӰεȽᆕኧ䜳丟ളޢਮ 213 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䚍Ֆ᭯㢥⍱ᙝ⭕⢟։ߒѐᓊ⭞⧥ູ伄䲟ᇗḛ㔉᷒ ᦤφדᗁ⌊ Ʌ⍱ᙝ᭯㢥⭕⢟։ㅿ䏀ູ䖢〱⌋Ɇㅢ 16 ᶗⅴδ伄䲟䇺զ䈘εૂㅢ 13 ᶗⅴδ伄 䲟䇺զညᢎᇔ᯳⍷ぁεχ Ʌ䪾ሯ⍱ᙝ᭯㢥⭕⢟։ㅿ䏀ູ䖢〱Ⲻ㔕жޢɆㅢ 1-4 ᶗδԙ㺞/ㆴ㓜ᵰᶺεㅢ ж⇫ૂ 4-8 ᶗδ⧥ູ伄䲟䇺զㅿεȾ 伄䲟ᇗḛᇯφ ⭧ӄɅ⍱։᭯㢥⭕⢟։ㅿ䏀ູ䖢〱⌋Ɇӄ 2008 ᒪ 1 ᴾ 1 ᰛᔶခᇔ᯳θ䘑ਙᡌ⭕ ӝ䖢ะഖ⭕⢟䴶㾷㔅䗽伄䲟ᇗḛχ ᣍ䲚㥿ࡸ⦿㊩ MON 87427 ⧥ູ伄䲟ᇗḛ⭩䈭ӄ 2011 ᒪ 6 ᴾ 28 ᰛᨆӚθ㔅䗽щ ᇬ䇺ᇗညՐⲺ 3 䖤ᇗḛȾ ᇗḛ㔉᷒ ᣍ䲚㥿ࡸ⦿㊩ MON 87427 ㊱ශ ӝ〦 ⭩䈭Ӱ ⢯ᙝ ᨆӚᰬ䰪 ᇗḛᇂᡆᰬ䰪 ᆕኧ䜳丟ള 2011 ᒪ 2013 ᒪ ⦿㊩ MON 87427 ᣍ䲚㥿ࡸ ޢਮ 6 ᴾ 28 ᰛ 12 ᴾ 16 ᰛ ṯᦤㅢ 101 ⅗⧥ູ伄䲟щᇬ䇺ᇗညՐՐ䇤δ2013 ᒪ 12 ᴾ 13 ᰛεⲺᇗḛ㔉᷒θ ߒᶇਇኋ㇗⨼ቶቶ䮵⺤䇚൞䘑ਙ⭞֒侨ᯏᡌ侨ᯏࣖᐛ㙂䶔ṳฯⲺ䗽ぁѣθྸ᷒ਇ ⭕ᰖᝅ⍷ᮙθ䚍Ֆ᭯㢥⦿㊩ MON 87427 ߒѐᓊ⭞ሯളߒѐ⧥ູуՐӝ⭕伄䲟Ⱦ 䱺Ԭφ䚍Ֆ᭯㢥⍱ᙝ⭕⢟։ߒѐᓊ⭞⧥ູ伄䲟ᇗḛ㔉᷒ 214 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䙐⸛㕌ਭ ߒѐ⍱ᙝ᭯㢥⭕⢟⧥ູ伄䲟ᇗḛ㔉᷒ 2013-07