Cp4 Epsps MON 87427

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䖜 cp4 epsps สഐ㙀䲔㥹ࡲ⦹㊣ MON 87427 䘋ਓ⭘ ֌࣐ᐕ৏ᯉⲴᆹޘ䇱Җ

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2017 ᒤ 6 ᴸ 20 ᰕ

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亩ⴞ਽〠˖ 䖜 cp4 epsps สഐ㙀䲔㥹ࡲ⦹㊣ MON 87427 䘋ਓ⭘֌࣐ ᐕ৏ᯉⲴᆹޘ䇱Җ

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ປᣕᰕᵏ˖2017 ᒤ 6 ᴸ 20 ᰕ

ѝॾӪ≁ޡ઼ഭߌъ䜘ࡦ ᆏኡ䜭ޜਨ᮷Ԧ

ᵜ⭣䈧ҖਇѝॾӪ≁ޡ઼ഭ㪇֌ᵳ⌅ǃц⭼⸕䇶ӗᵳ㓴㓷˄WIPO˅㪇֌ᵳޜ㓖઼ ˈᣔǄᵜ⭣䈧Җ৺ަ䱴ᑖᶀᯉӵ׋᧕᭦Ⲵ⌅㿴ᵪᶴᡰ⭘؍㓖Ⲵޜ૱WIPO 㺘╄৺ᖅ丣ࡦ ᒦӵ⭘Ҿ᭟ᤱᆏኡ䜭ޜਨⲴ⴨ᓄ⭣䈧Ǆ䲔䶎⴨ޣ⌅ᖻ⌅㿴৺⌅㿴ѫ㇑䜘䰘㿴ᇊˈަԆ ԫօሩᵜ⭣䈧Җ৺ަ䱴ᑖ䍴ᯉⲴަԆᓄ⭘ǃᤧ䍍৺वᤜӂ㚄㖁ޜᐳ൘޵ⲴՐ᫝䜭ᗵ享 ਨⲴҖ䶒਼᜿Ǆᵜ⭣䈧ѝ᧿䘠Ⲵᡆ䱴ᑖⲴ⸕䇶ӗᵳǃؑ᚟ԕ৺ᶀᯉޜһݸᗇࡠᆏኡ䜭 ᐢ㓿⭡ᆏኡ䜭ޜਨ⭣ᣕу࡙ᡆ㘵㧧ᗇу࡙ˈѪᆏኡ䜭ޜਨᡰᴹǄᆏኡ䜭ޜਨᨀӔᵜ⭣ ਨሶᵜ⭣䈧Җѝ᧿䘠ᡆवਜ਼Ⲵᶀᯉǃؑ᚟ᡆ⸕䇶ӗᵳᦸᵳ㔉ަԆޜ䈧Җн㺘⽪ᆏኡ䜭 ԫօњӪᡆᇎփǄ ມ ߏ 䈪 ᱄ ㇗⨼ᶗׁɆȽɅߒѐ䖢ޞ1κ൞ມߏ⭩ᣛҜҁࢃθᓊ䇚ⵕ䰻䈱Ʌߒѐ䖢ะഖ⭕⢟ᆿ ะഖ⭕⢟ᆿޞ䇺ԭ㇗⨼ࣔ⌋ɆȽɅߒѐ䖢ะഖ⭕⢟䘑ਙᆿޞ㇗⨼ࣔ⌋Ɇㅿᴿީ⌋㿺θ Ҽ䀙⴮ީ㾷≸Ⱦ 2κ↚⭩ᣛҜੂṭ䘸⭞ӄ⌋㿺㿺ᇐⲺᣛ઀㊱ᇔ僂⹊ガૂѣ䰪䈋僂Ⲻᣛ઀θ੃〦࠼ࡡ ᭯Ѱ“ߒѐ䖢ะഖ⭕⢟ᇔ僂⹊ガᣛ઀Ҝ ”ૂ“ߒѐ䖢ะഖ⭕⢟ѣ䰪䈋僂ᣛ઀Ҝ ”Ⱦ 3κ⭩ᣛҜ޻ᇯᓊᖉऻᤢԛс䜞࠼φ⭩䈭㺞Ƚ亯ⴤ޻ᇯ᪎㾷Ƚᐛ֒ⴤⲺૂᝅѿȽള ޻ཌ⹊ガⲺ⴮ީ㜂Ქ䍺ᯏȽᆿޞᙝ䇺ԭȽ䈋僂ᯯṾȽ⴮ީ䱺Ԭ䍺ᯏȽᵢঋփߒѐ䖢ะ ഖ⭕⢟ᆿޞቅ㓺ᇗḛᝅ㿷Ƚᵢঋփᇗḛᝅ㿷Ƚᡶ൞ⴷδᐸȽ㠠⋱॰εⲺߒѐ㺂᭵ѱ㇗䜞䰞Ⲻᇗḛᝅ㿷Ⱦ 4κ⭩䈭ᇔ僂⹊ガⲺθ⭩䈭㺞᤿㺞 2 ມߏθ亯ⴤ੃〦ᓊऻ੡䖢ะഖᙝ⣬Ƚ਍։⭕⢟ ੃〦Ƚᇔ僂⹊ガᡶ൞ⴷδᐸȽ㠠⋱॰ε੃〦ૂᇔ僂⹊ガ䱬⇫ㅿ޻ᇯθྸ “䖢ะഖᣍ㲡ỿ 㣧൞⋩्ⴷⲺᇔ僂⹊ガ ”ȾжԳ⭩ᣛҜਠ㜳ऻ੡ੂж⢟〃Ⲻ਍։⭕⢟ૂ⴮ੂⲺ䖢ะഖᙝ ⣬Ⱦ“ᆿޞᙝ䇺ԭ ”ਥуມߏȾ “䈋僂ᯯṾ”ऻᤢ⹊ガⴤḽȽཌⓆะഖⲺ੃〦ૂᶛⓆȽ䖳 ։ᶺᔰഴ䉧Ƚ䖢ौᯯ⌋ૂ㿺⁗Ƚ൦⛯Ƚᆿޞ᧝࡬᧠᯳ㅿȾ “⴮ީ䱺Ԭ䍺ᯏ ”ऻᤢ⌋Ӱ䇷 Ҝૂ㩛ѐᢝ➝ⲺགྷদԬθ 㤛⎿਀ਾ֒亯ⴤθ ᓊᨆӚਂᯯਾ֒ᡌ䖢䇟অ䇤ㅿȾ “ᡶ൞ⴷδᐸȽ 㠠⋱॰εⲺߒѐ㺂᭵ѱ㇗䜞䰞Ⲻᇗḛᝅ㿷 ”ਥуມߏθ⭩ᣛᶆᯏᣛ䘷ߒѐ䜞ᰬθᢺ䘷ᇔ 僂⹊ガᡶ൞ⴷδᐸȽ㠠⋱॰εⲺߒѐ㺂᭵ѱ㇗䜞䰞Ⱦ 5κ⭩䈭ѣ䰪䈋僂Ⲻθ⭩䈭㺞᤿㺞 2 ມߏȾ⭩ᣛҜѣ“ᆿޞᙝ䇺ԭ ”Ƚ“䈋僂ᯯṾ ”ૂ 䇺ԭ㇗⨼ࣔ⌋Ɇ䱺ᖋ IȽIIȽIII 㾷≸䙆ᶗມޞɅߒѐ䖢ะഖ⭕⢟ᆿ➝ד” ⴮ީ䱺Ԭ䍺ᯏ“ ߏȾ“ᡶ൞ⴷδᐸȽ㠠⋱॰ε㓝ߒѐ㺂᭵ѱ㇗䜞䰞Ⲻᇗḛᝅ㿷 ”ਥуມߏθ⭩ᣛᶆᯏᣛ 䘷ߒѐ䜞ᰬθᢺ䘷ѣ䰪䈋僂ᡶ൞ⴷδᐸȽ㠠⋱॰ε㓝ߒѐ㺂᭵ѱ㇗䜞䰞Ⱦ 6κ⭩䈭⧥ູ䠀᭴ૂ⭕ӝᙝ䈋僂Ⲻθ⭩䈭㺞᤿㺞 2 ມߏχ⭩ᣛҜѣ “ᆿޞᙝ䇺ԭ ”Ƚ 䇺ԭ㇗⨼ࣔ⌋Ɇ䱺ᖋ IȽIIȽޞɅߒѐ䖢ะഖ⭕⢟ᆿ➝ד” 䈋僂ᯯṾ ”ૂ“⴮ީ䱺Ԭ䍺ᯏ“ III 㾷≸䙆ᶗມߏȾሯӄᐨ᢯߼䗽Ⲻ⧥ູ䠀᭴ᡌ⭕ӝᙝ䈋僂θ൞䈋僂㔉ᶕ੄θ㤛ੂж䖢 ะഖ⭕⢟൞৕᢯߼൦⛯ԛ⴮ੂ㿺⁗䠃གྷ䈋僂θ൞⭩ᣛᰬ “ᆿޞᙝ䇺ԭ ”䜞࠼ਥⴷ⮛Ⱦ 7κ⭩䈭ߒѐ䖢ะഖ⭕⢟ᆿޞ䇷ҜⲺθ⭩䈭㺞᤿㺞 3 ມߏχ⭩ᣛҜѣ “ᆿޞᙝ䇺ԭ ” 䇺ԭ㇗⨼ࣔ⌋Ɇ䱺ᖋ IȽIIȽIII 㾷≸䙆ᶗޞɅߒѐ䖢ะഖ⭕⢟ᆿ➝ד” ⴮ީ䱺Ԭ䍺ᯏ“ૂ ມߏθ“䈋僂ᯯṾ ”у⭞ມߏχ 䖢ะഖὃ⢟Ⲻ૷〃ળ੃ᓊㅜਾ Ʌߒѐὃ⢟૷〃ળ੃㿺ᇐɆ χ Ʌߒѐ䖢ะഖב䇷ҜⲺθ䴶ᨆޞ俌⅗⭩䈭ߒѐ䖢ะഖ⭕⢟૷〃δᡌ૷㌱ε⭕ӝᓊ⭞ᆿ ⭕⢟૷〃δᡌ૷㌱ε⭕ӝᓊ⭞㔲ਾ䇺ԭᣛ઀Ɇθѱ㾷ऻᤢሯᡇള⭕ӝȽ䍮᱉Ƚ⽴Րㅿ ᯯ䶘ᖧଃȾ 䇷ҜⲺθ൞⭩䈭ᡠ↘ᰛ 30 Ѡᐛ֒ޞ8κ俌⅗⭩䈭ߒѐ䖢ะഖ⭕⢟⭕ӝᙝ䈋僂ૂᆿ ᡶ⭩ᣛ䖢ะഖ⭕⢟⍱ᙝṭ૷ૂᢶᵥ䍺ᯏȾṭ૷㾷≸φㅜਾ㾷≸Ⲻθᴿ⍱ᙝⲺבᰛࢃᨆ ὃ⢟〃ᆆ 2.5 kgθࣞ⢟ 5ml 㹶ṭᡌ 3g 㓺㓽਺пԳθᗤ⭕⢟ 3 ᢯⅗θ਺ 10 ㇗ṭ૷Ⱦᢶ ཌⓆ⡽⇫᮪ਾ䘑ะഖ㓺Ⲻ Southern ᵸӚ㔉᷒Ƚ᤭䍓בᵥ䍺ᯏ㾷≸φ⭩䈭⭕ӝᙝ䈋僂ᨆ ⌊ؗᚥ਀䖢ौ։⢯ᔸᙝ PCR Ỷ⎁ᯯ⇫⡽ޛཌⓆᨈב䇷Ҝᨆޞᮦ਀Ỷ⎁ᯯ⌋ㅿχ ⭩䈭ᆿ ㅿȾ 9κ⭩ᣛҜᓊᖉ⭞ѣᮽມߏθжᕅॷԳθжᗁֵ⭞ A4 㓮θ↙ᮽ⭞ቅ഑ᇁ։ᢉদθ ݿⴎȾሯӄуㅜਾ㾷≸Ⲻ⭩ᣛҜθуҾ਍⨼Ⱦב㺂䐓θᒬᨆكḽ߼ᆍ䰪䐓ૂঋ 10κ⭩䈭㘻ਥԛ⌞᱄ଠӑ䍺ᯏ䴶㾷ؓᇼᒬ䈪᱄⨼⭧Ⱦ 11κ਍⨼ᰬ䰪φ∅ᒪ 3 ⅗θᡠ↘ᰛᵕ࠼ࡡѰ 3 ᴾνᰛȽ 7 ᴾ 1 ᰛૂ 11 ᴾ 1 ᰛȾ 12κ਍⨼ঋփφߒѐ䜞㺂᭵ᇗ᢯㔲ਾࣔޢᇚ〇ᮏネਙȾ൦൶φ्Ӣᐸᵓ䱩॰ߒኋ 侼঍䠂 11 ਭȾ䛤᭵㕌⸷φ 100125Ⱦ᭬ⅴঋփφߒѐ䜞〇ᢶਇኋѣᗹχᔶᡭ㺂φѣؗ䬬㺂्Ӣᵓ䱩᭥㺂χᑆਭφ 7111110189800000419Ⱦ ⴞ ᖅ

аǃ⭣䈧㺘...... 1 Ҽǃ亩ⴞ޵ᇩ᪈㾱...... 9 йǃᐕ֌ⴞⲴ઼᜿ѹ ...... 12 ഋǃഭ޵ཆ⴨ޣ⹄ウⲴ㛼Ჟ䍴ᯉ ...... 13 ӄǃ䖜สഐἽ⢙Ⲵᆹޘᙗ䇴ԧ ...... 20 1. ਍։ὃ⢟Ⲻᆿޞᙝ䇺ԭ...... 20 ᙝ䇺ԭ...... 35ޞะഖᬃ֒Ⲻᆿ .2 3. 䖢ะഖὃ⢟ᆿޞᙝ䇺ԭ...... 68 4. 䖢ะഖὃ⢟ӝ૷ᆿޞᙝ䇺ԭ...... 128 ޝǃ⴨ޣ䱴Ԧ䍴ᯉ...... 141 1. ⴤⲺะഖⲺṮ㤭䞮ᓅࡍ਀ެ᧞ሲⲺ≞ะ䞮ᓅࡍ...... 142 2. ⴤⲺะഖф䖳։ᶺᔰⲺഴ䉧...... 143 3. ⴤⲺะഖфὃ⢟ะഖ㓺᮪ਾ਀ެ㺞䗴Ⲻ࠼ᆆỶ⎁ᡌ䢪ᇐ㔉᷒...... 146 4. 䖢ะഖᙝ⣬਀ӝ⢟ⲺỶ⎁ૂ䢪ᇐᢶᵥ...... 171 5. ਺䈋僂䱬⇫ᇗ᢯ҜⲺགྷদԬ...... 174 6. ਺䈋僂䱬⇫Ⲻᆿޞᙝ䇺ԭ䈋僂ᙱ㔉ᣛ઀...... 174 7. 䖢ะഖὃ⢟ሯ⭕ᘷ⧥ູᆿޞᙝⲺ㔲ਾ䇺ԭᣛ઀...... 174 8. 伕૷ᆿޞᙝⲺ㔲ਾ䇺ԭᣛ઀...... 174 9. 䈛㊱䖢ะഖὃ⢟ള޻ཌ⭕ӝᓊ⭞Ᾰ߫...... 182 10. ⭦䰪ⴇ᧝ᯯṾ...... 268 11. ᇗḛᡶ䴶Ⲻެᆹ⴮ީ䍺ᯏ ...... 269 䖢ะഖ⭕⢟൞䗉࠰ളᇬᡌ൦॰ᐨ㔅ݷ䇮֒Ѱ⴮ᓊ⭞䙊ᒬᣋ᭴ᐸ൰Ⲻ .12 䇷᱄ᮽԬ...... 271 13. 䗉࠰ളᇬᡌ൦॰㔅䗽〇ᆜ䈋僂䇷᱄ሯӰ㊱Ƚࣞὃ⢟ૂ⭕ᘷ⧥ູᰖᇩⲺ 䍺ᯏ...... 290 гǃᵜঅսߌъ䖜สഐ⭏⢙ᆹޘሿ㓴ᇑḕ᜿㿱...... 292 ޛǃᵜঅսᇑḕ᜿㿱 ...... 293 ҍǃᡰ൘ⴱ˄ᐲǃ㠚⋫४˅Ⲵߌъ㹼᭯ѫ㇑䜘䰘Ⲵᇑḕ᜿㿱...... 294 ߌъ䜘ޣҾMON 87427ᆹޘ䇴ԧ⭣䈧Ⲵഎ༽࠭ ˄2017ᒤ6ᴸ12ᰕ˅

I ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ҿߌъ䜘ᢩ༽ؑ࠭Ⲵ䈤᰾ޣਨޜᆏኡ䜭

䰞仈˖ሩҾ 2016 ᒤ 12 ᴸᢩ༽᮷ԦⲴഎ༽޵ᇩѝˈᵚᨀ׋㖾ഭᐢਁ⧠Ⲵ 17 ⿽㥹 ⭈㟖ᣇᙗᵲ㥹Ⲵ਽〠ǃᣇᙗᵲ㥹Ⲵ䲔㥹ࡲ⭘䟿৺ަ↻⮉䟿ਈॆǄ 䈤᰾˖ᵲ㥹ሩ䲔㥹ࡲⲴᣇᙗаⴤᱟߌъ⭏ӗѝⲴаབྷ䰞仈ǄሩҾԫօа⿽䲔㥹ࡲ 㘼䀰ˈ৽༽䮯ᵏঅа֯⭘㘼н䖵ԕަԆᵲ㥹᧗ࡦ᡻⇥ᴰ㓸䜭Պሬ㠤ᣇᙗᵲ㥹㗔փ Ⲵӗ⭏Ǆᵲ㥹Ⲵ䲔㥹ࡲᣇᙗ䰞仈㠚кц㓚ӄॱᒤԓ䎧ቡᕅ䎧Ҷޣ⌘ˈᒦ䶎ᱟ䲿⵰ 㙀䲔㥹ࡲ䖜สഐ֌⢙㘼ࠪ⧠Ⲵᯠ䰞仈Ǆ ⴞࡽ൘㖾ഭᣕ੺ਁ⧠Ⲵ㥹⭈㟖ᣇᙗᵲ㥹ޡ 17 ⿽ˈަ਽〠䈧㿱л㺘Ǆ㔏䇑ᮠ ᦞᶕⓀҾഭ䱵䲔㥹ࡲᣇᙗᵲ㥹䈳ḕ㖁ㄉ http://www.weedscience.org/Ǆ

᣹бᆖ਽ 㤡᮷਽ ѝ᮷਽ ਁ⧠ൠ֌⢙㊫ර 1 Amaranthus palmeri Palmer 䮯㣂㣻 ⦹㊣ǃỹ㣡ǃབྷ䉶 Amaranth 2 Amaranthus spinosus Spiny Amaranth ࡪ㣻 ỹ㣡 3 Amaranthus Tall Waterhemp ㌉᷌㣻 ⦹㊣ǃỹ㣡ǃབྷ䉶ǃ tuberculatus ˄=㾯䜘㣻˅ 儈㋡ (=A.rudis) 4 Ambrosia Common 䊊㥹 ⦹㊣ǃỹ㣡ǃབྷ䉶 artemisiifolia Ragweed 5 Ambrosia trifida Giant Ragweed й㻲ਦ䊊㥹 ⦹㊣ǃỹ㣡ǃབྷ䉶 6 Conyza bonariensis Hairy Fleabane 俉э㥹 ⦹㊣ǃ㪑㨴ǃ᷌ഝ 7 Conyza canadensis Horseweed ሿ㬜㥹 ⦹㊣ǃỹ㣡ǃབྷ䉶ǃ ሿ哖ǃᵿӱ Echinochloa colona Junglerice ݹཤぇ ⦹㊣ǃỹ㣡ǃ㪑㨴ǃ 8 ᷌ഝ 9 Eleusine indica Goosegrass ⢋ㅻ㥹 ỹ㣡ǃབྷ䉶ǃ䉧⢙ 10 Helianthus annuus Common ੁᰕ㪥 ⦹㊣ Sunflower 11 Kochia scoparia Kochia ൠ㛔 ⦹㊣ǃỹ㣡ǃབྷ䉶ǃ ⭈㭇 12 Lolium perenne ssp. Italian Ryegrass ཊ㣡唁哖㥹 ⦹㊣ǃỹ㣡ǃབྷ䉶ǃ multiflorum ሿ哖ǃ᷌ഝǃ㪑㨴ǃ 㤌㬯 13 Lolium rigidum Rigid Ryegrass ⺜ⴤ唁哖㥹 ᵿӱǃ᷌ഝ 14 Poa annua Annual ᰙ⟏⿮ ᵿӱǃ㥹ක Bluegrass 15 Parthenium Ragweed 䬦㜦㧺䶎֌⢙⿽Ἵ४˄ྲ

II ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 hysterophorus Parthenium 䐟䗩ǃ䫱䖘˅ 㖇ᯟ㬏 ሿ哖״ Salsola tragus Russian-thistle 16 17 Sorghum halepense Johnsongrass ⸣㤵 བྷ䉶

к㺘ѝᡰࡇⲴ㥹⭈㟖ᣇᙗᵲ㥹བྷ䜘࠶ᱟ൘䮯ᵏԕḷㆮࡲ䟿௧ᯭ㥹⭈㟖Ⲵߌ ⭠ѝਁ⧠Ⲵˈ㥹⭈㟖Ⲵާփ௧ᯭࡲ䟿ഐߌ⭠ѝ⿽ἽⲴ֌⢙㘼ᔲˈঅ⅑௧ᯭᴰ儈ࡲ 䟿ਟ䗮 1.5 lbs. a.e./A.Ǆл㺘ѝᾲ䘠Ҷн਼ᣇ㥹⭈㟖֌⢙Ⲵḷㆮ௧ᯭᴰ儈ࡲ䟿˖

অ⅑௧ᯭᴰ儈ࡲ䟿 ᙫ௧ᯭᴰ儈ࡲ䟿 ᣇ㥹⭈㟖֌⢙ ˄֌⢙㹼䰤/䎺亦௧ᯭ˅ ˄֌⢙㹼䰤/䎺亦௧ᯭ˅ (lb. a.e./A) (lb. a.e./A) ᣇ㥹⭈㟖㤌㬯 1.55 4.61 ᣇ㥹⭈㟖⋩㨌 0.56 0.77 ˄᱕⋩㨌˅ ᣇ㥹⭈㟖⋩㨌 0.77 1.55 ˄ߜ⋩㨌˅ ᣇ㥹⭈㟖⋩㨌 1.55 1.55 ˄MON 88302˅ ᣇ㥹⭈㟖བྷ⭠⦹ 1.125 2.25 ㊣˄NK603˅ 0.77˄᭦㧧ࡽ௧ᯭ˅ 2.32˄ྲ᷌᭦㧧ࡽ࣐௧а⅑˅ ᣇ㥹⭈㟖⭌⦹㊣ 1.55 4.64 ˄NK603˅ ᣇ㥹⭈㟖ỹ㣡 0.77 2.8125 ˄MON 1445˅ 1.125˄ӊ࡙ẁ䛓ᐎ˅ 4.359˄ྲ᷌᭦㧧ࡽ࣐௧а⅑˅ 1.55˄᭦㧧ࡽ௧ᯭ˅ ᣇ㥹⭈㟖ỹ㣡 1.125 ˄MON 88913˅ 1.55˄ӊ࡙ẁ䛓ǃᯠ໘㾯 4.50 ˅କᐎǃ㾯ᗧݻ㩘ᯟᐎ˅ 6.05˄ྲ᷌᭦㧧ࡽ࣐௧а⅑ 1.55˄᭦㧧ࡽ௧ᯭ˅ ᣇ㥹⭈㟖བྷ䉶 1.55 2.25 ˄MON 89788˅ ᣇ㥹⭈㟖⭌㨌 1.125 3.5156

ᵲ㥹Ⲵ䲔㥹ࡲᣇᙗ㻛ᇊѹѪḀ⿽䲔㥹ࡲⴞḷᵲ㥹ѝⲴḀа㗔փ㧧ᗇҶ㙀ਇ 䘉⿽䲔㥹ࡲⲴ㜭࣋ˈ൘ԕᖰⲴ௧ᯭࡲ䟿л䈕䲔㥹ࡲሩ⢩ᇊ㗔փᵲ㥹н޽ᴹ᭸ ˄WSSA, 2011˗HRAC, 2016˅ˈࡽ㺘ѝᡰࡇᵲ㥹ণᱟ൘ḷㆮࡲ䟿௧ᯭਾᆈ⍫Ⲵ ᣇᙗ㗔փǄ᤹➗ഭ䱵䲔㥹ࡲᣇᙗᵲ㥹䈳ḕ㖁ㄉⲴ༠᰾ˈᨀ׋ࡠ䈕㖁ㄉᒦ༠〠Ѫ“ᣇ ᙗᵲ㥹”Ⲵ㗔փᗵ享㓿䗷䘋а↕Ⲵ⍻䈅ˈ᧘㦀᧗ࡦᶑԦ˄ษޫ㇡ǃ⑙ᇔ˅лⲴἽ Ṛࡲ䟿૽ᓄ䈅傼˄http://www.weedscience.org/Documents/ResistanceCriterion.pdf˅Ǆ 䘹㗔փ࠶࡛⭘վҾ઼儈ҾḷㆮⲴࡲ䟿௧ᯭⴞḷ䲔ى൘ࡲ䟿૽ᓄ⹄ウѝˈᣇᙗᵲ㥹 III ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㥹ࡲˈ䇴ՠᵲ㥹ἽṚⲴᆈ⍫㺘⧠Ǆࡽ㺘ѝᡰࡇ㖁ㄉѝⲴᵲ㥹ᮠᦞ⭡н਼Ⲵ⹄ウ㘵 ᨀ׋ˈަ⹄ウᰦ֯⭘Ⲵ௧ᯭࡲ䟿ҏнቭ⴨਼Ǆ∄ྲˈNandula ㅹӪሩ㌉᷌㣻 ˄Amaranthus tuberculatus˅䘋㹼Ⲵ⹄ウѝˈ㥹⭈㟖௧ᯭࡲ䟿㤳തѪ 0.21-3.36 kg a.e. ha-1˄http://www.bioone.org/doi/full/10.1614/WS-D-12-00155.1˅ˈ㘼 Bell ㅹӪ ਼ṧሩ㌉᷌㣻䘋㹼Ⲵ⹄ウѝˈ㥹⭈㟖Ⲵ௧ᯭࡲ䟿㤳തࡉѪ 0.052 to 6.7 kg a.e. ha-1 http://pubs.acs.org/doi/abs/10.1021/jf103797n˅Ǆє亩⹄ウ൷㺘᰾ˈণ֯൘⍻䈅˄ ᡰ⭘ᴰ儈ࡲ䟿лˈ㥹⭈㟖ሩ䘉Ӌᵲ㥹Ⲵ䲔㥹᭸᷌൷∄ሩ➗㓴ᴹᡰ߿ᕡǄᰐ䇪ᱟ൘ ࡲ䟿૽ᓄ⹄ウ઼ᑨ㿴Ⲵ䲔㥹ࡲ㦟᭸઼ᣇᙗ⹄ウѝˈ䙊ᑨ䜭нՊሩᵲ㥹ѝⲴ䲔㥹ࡲ ↻⮉䟿䘋㹼Ự⍻ˈަѝа䜘࠶৏ഐᱟ᱃ᝏᵲ㥹Պྲ亴ᵏ㻛䲔㥹ࡲᵰ↫ˈ㘼䲔㥹ࡲ 㦟᭸઼Ἵ⢙䮯࣯ࡉᱟєњᴤᑨ㿱Ⲵ⍻䟿ᤷḷǄഐ↔ˈ㥹⭈㟖൘ᵲ㥹ѝ↻⮉䟿ⲴỰ ⍻ᮠᦞ䶎ᑨᴹ䲀Ǆ Ѫ⺞ᇊ⢩ᇊ䲔㥹ࡲ൘Ḁ⿽Ἵ⢙ѝⲴ↻⮉䟿ˈỰ⍻ᯩ⌅ᗵ享㓿䗷傼䇱ˈ⎸䲔 н਼ส䍘ӗ⭏Ⲵ䈟ᐞˈԕ㧧ᗇਟ䶐ⲴỰ⍻㔃᷌Ǆ❦㘼ˈสҾᡁԜӾк䘠ഭ䱵䲔㥹ࡲᣇᙗᵲ㥹䈳ḕ㖁ㄉѝ㧧ᗇⲴؑ᚟ˈ䪸ሩ䘉 17 ⿽㻛ᣕ੺Ѫާᴹ㥹⭈㟖ᣇᙗⲴ ᵲ㥹ˈ勌ᴹ⢩࡛䪸ሩḀ⿽ᵲ㥹㘼䇮䇑Ⲵ䲔㥹ࡲ↻⮉Ự⍻ᯩ⌅Ǆᵲ㥹、ᆖ亶ฏⲴ ⹄ウ㘵ਟ㜭Պሩᣇᙗᵲ㥹ѝ䲔㥹ࡲⲴ↻⮉䟿ᝏޤ䏓ᒦ䘋㹼⍻䈅ˈնሩҾ䘋ਓⲴ ˈⲴ䇱ᦞޣᙗ⴨ޘབྷᇇߌӗ૱㘼䀰ˈ䘉㊫ᮠᦞᰐ⌅ᨀ׋ᴤཊо䖜สഐ֌⢙伏⭘ᆹ 䘉ҏᱟ㥹⭈㟖൘ᵲ㥹ѝ↻⮉䟿ᮠᦞ䶎ᑨቁⲴ৏ഐѻаǄ 㲭❦㥹⭈㟖ᱟ䶎䘹ᤙᙗ䲔㥹ࡲˈնྲࡽᡰ䘠ˈ䮯ᵏ৽༽֯⭘অа䲔㥹ࡲᴰ㓸 ∄˄䜭Պሬ㠤ᣇᙗᵲ㥹Ⲵӗ⭏Ǆᖃߌ≁᜿䇶ࡠ㔗㔝֯⭘ḷㆮࡲ䟿௧ᯭḀ⿽䲔㥹ࡲ ྲ㥹⭈㟖˅൘㠚ᇦߌ⭠Ⲵ䲔㥹᭸᷌ᐢнྲԕᖰᰦˈԆԜᗵ享䘹ᤙަԆᯩᔿ৫䲔䘉 Ӌ亭പⲴᵲ㥹ˈवᤜ֯⭘ަԆ㊫රⲴ䲔㥹ࡲᡆᱟ֯⭘⢙⨶䲔㥹ᯩᔿˈ∄ྲ㘫㙅ᡆ 䬴㥹Ǆ㥹⭈㟖ሩߌ⭠ѝަԆⲴ᱃ᝏᵲ㥹ӽ❦ᴹ᭸ˈնণ֯ߌ≁൘䘹ᤙަԆ䲔㥹ᯩ ˈᔿⲴ਼ᰦ㔗㔝֯⭘㥹⭈㟖ˈԆԜҏнՊ䙊䗷ᨀ儈௧ᯭࡲ䟿ᶕ䈅മᵰ↫ᣇᙗᵲ㥹 ↔䇱ᣇᙗᵲ㥹аᇊՊ㻛ᵰ↫Ǆ؍⌅ҏᰐˈ؍⧟ഐѪᨀ儈ࡲ䟿Ⲵᯩ⌅ᰒн㓿⍾ҏн ተѕṬ㇑⨶ᡰᴹߌ㦟Ⲵ֯⭘ḷㆮˈᤷᇊ⇿а⿽ߌ㦟ӗ૱൘ⴞⲴ⭘䙄؍⧟ཆˈ㖾ഭ ⌅кⲴ֯⭘ᯩ⌅ˈवᤜᴰվ઼ᴰ儈֯⭘ࡲ䟿Ǆԫօнㅖਸḷㆮ㿴ᇊⲴ֯⭘൷Ѫ䘍㹼ѪǄഐ↔ˈ൘ਸ⌅֯⭘Ⲵࡽᨀлˈਟ亴ᵏ֌⢙᭦㧧㊭㋂ᡆᱟᣇᙗᵲ㥹ѝⲴ䲔㥹 ࡲ↻⮉䟿ᒦнՊഐѪߌ⭠ѝᵲ㥹ᱟ੖ާᴹᣇᙗ㘼ਈॆǄ MON 87427 ᱟᆏኡ䜭ޜਨ᧘ࠪⲴ㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣૱⿽ˈަ䳴ᙗಘ ᇈᒦн㙀ਇ㥹⭈㟖ˈഐ↔௧ᯭਜ਼ᴹ㥹⭈㟖Ⲵ䲔㥹ࡲਾਟ䗮ࡠᘛ䙏ᖫᓅⲴ৫䳴᭸᷌Ǆ ަӗ૱ⴞⲴᱟ֌Ѫ⇽ᵜ⭘Ҿࡦ⿽ˈ㘼䶎⭠䰤⿽Ἵˈഐ↔ᒦнՊ໎࣐ߌ⭠ѝ⧠ᴹ㥹 ⭈㟖ᣇᙗᵲ㥹Ⲵ䘹ᤙ঻࣋Ǆ਼ṧˈ൘ԕ MON 87427 Ѫ⇽ᵜᗇࡠⲴᵲӔ⿽ਾԓ⭏ ⢙ተᢩ߶Ⲵ㥹⭈㟖ḷㆮ֯⭘ࡲ䟿䘋㹼֌؍⧟䮯Ⲵߌ⭠ѝˈ㖾ഭߌ≁ᗵ享᤹➗㖾ഭ 㹼䰤/䎺亦௧ᯭ䲔㥹ˈഐ↔ˈ⦹㊣㊭㋂৺ަ࣐ᐕ㓴࠶ѝⲴ㥹⭈㟖↻⮉䟿亴ᵏнՊ ഐᆈ൘ᣇᙗᵲ㥹㘼ਁ⭏ਈॆˈߌ⭠ѝԫօਟ㜭ᆈ൘Ⲵ㥹⭈㟖ᣇᙗᵲ㥹ѝⲴ↻⮉䟿 ҏᱟྲ↔Ǆ ᆏኡ䜭ޜਨ࡙⭘㓿傼䇱ᴹ᭸Ⲵ⍻䈅ᯩ⌅Ự⍻Ҷਜ਼ᴹ MON 87427 ᙗ⣦Ⲵ⦹㊣㊭㋂ѝ㥹⭈㟖Ⲵ↻⮉≤ᒣǄѪᗇࡠ⦹㊣⋩઼⦹㊣㊹ѝ㥹⭈㟖↻⮉≤ᒣ⴨ሩҾ㊭㋂ Ⲵ⎃㕙㌫ᮠ˄CF˅ˈ⢩࡛ሶᇎ傼ᶑԦѝⲴ௧ᯭࡲ䟿࣐བྷ˄~2 ؽ˅ˈণ֯൘䘉⿽

IV ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ࣐བྷࡲ䟿ᶑԦлˈਜ਼ᴹ MON 87427 ᙗ⣦Ⲵ⦹㊣㊭㋂ѝ㥹⭈㟖Ⲵ↻⮉䟿Ѫ 0.91 ppmˈ䘌վҾ CODEX ൘⦹㊣㊭㋂к䇮ᇊⲴ㥹⭈㟖↻⮉䲀䟿ḷ߶˄5 ppm˅˄Codex Alimentarius, 2016˅ˈ䈧㿱ᢰᵟᣕ੺ 16bǄഐ↔ˈণ֯ᴹњ࡛ߌ≁䈅മ䙊䗷ᨀ儈 ࡲ䟿ᶕᵰ↫ᣇᙗᵲ㥹ˈ亴ᵏ⦹㊣㊭㋂ᡆᵲ㥹ѝⲴ㥹⭈㟖↻⮉≤ᒣҏнՊ䎵䗷 ٬Ǆ CODEX 䇮ᇊⲴ MRL ࡽᡰ䘠ˈሩҾԫօа⿽䲔㥹ࡲ㘼䀰ˈ৽༽䮯ᵏঅа֯⭘㘼н䖵ԕަԆᵲ㥹ྲ ᧗ࡦ᡻⇥ᴰ㓸䜭Պሬ㠤ᣇᙗᵲ㥹㗔փⲴӗ⭏Ǆഐ↔ˈᣇཊ⿽䲔㥹ࡲⲴ༽ਸᙗ⣦䖜 สഐ૱⿽ҏᱟа⿽ᴹ᭸䱢→ᣇᙗᵲ㥹ӗ⭏Ⲵ䖳ྭ䘹ᤙˈⴞࡽᐢᴹ䎺ᶕ䎺ཊⲴ䘉㊫ ӗ૱䰞цǄ㘼 MON 87427 ਚᱟᆏኡ䜭ޜਨ᧘ࠪⲴ㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣૱⿽ˈ ަ䳴ᙗಘᇈᒦн㙀ਇ㥹⭈㟖ˈഐ↔௧ᯭਜ਼ᴹ㥹⭈㟖Ⲵ䲔㥹ࡲਾਟ䗮ࡠᘛ䙏ᖫᓅⲴ ৫䳴᭸᷌Ǆ㲭❦ MON 87427 ⦹㊣ҏ㜭ཏ㺘䗮 CP4 EPSPS 㳻ⲭˈնަӗ૱ⴞⲴᒦ 䶎ѪҶ൘୶ъॆ⿽Ἵᰦ䙊䗷௧ᯭ㥹⭈㟖᧗ࡦߌ⭠ᵲ㥹ˈ㘼ਚᱟ֌Ѫ⇽ᵜ⭘Ҿࡦ⿽ˈ ഐ↔ᒦнՊ໎࣐ߌ⭠ѝ⧠ᴹ㥹⭈㟖ᣇᙗᵲ㥹Ⲵ䘹ᤙ঻࣋Ǆ

V ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߌъ䜘ޣҾMON 87427ᆹޘ䇴ԧ⭣䈧Ⲵഎ༽࠭ ˄2016ᒤ12ᴸ31ᰕ˅

VI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ҿߌъ䜘ᢩ༽ؑ࠭Ⲵ䈤᰾ޣਨޜᆏኡ䜭

䰞仈 1˖ ᵚᨀ׋㖾ഭߌ⭠ᵲ㥹ሩ㥹⭈㟖䲔㥹ࡲ㙀ਇᙗ⧠⣦᧿䘠઼࠶᷀Ⲵ䈖㓶䍴ᯉ˗

䈤᰾˖㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣ MON 87427 ѝᕅޕҶⓀ㠚Ҿ൏༔ߌᵶ㧼 CP4 㧼 ṚⲴ cp4 epsps 㕆⸱ᒿࡇˈަ㺘䗮ӗ⢙ 5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦 ˄CP4 EPSPS˅ሩ㥹⭈㟖ⲴӢ઼ᙗ䶎ᑨվˈഐ↔㜭ཏ㙀ਇ㥹⭈㟖㊫䲔㥹ࡲǄ

㥹⭈㟖㊫䲔㥹ࡲᐢ㓿୶ъॆᓄ⭘Ҷ䎵䗷40ᒤˈ⢩࡛ᱟ㠚кц㓚90ᒤԓԕᶕˈ 䲿⵰ᣇ㥹⭈㟖֌⢙୶ъॆ⿽ἽⲴнᯝᢙབྷˈ㥹⭈㟖ᐢ㓿ᡀѪߌ≁ᴰѫ㾱ǃᴰᴹ᭸ Ⲵߌ⭠ᵲ㥹᧗ࡦ᡻⇥ѻаǄդ䲿⵰㥹⭈㟖Ⲵ䮯ᵏᓄ⭘ˈṩᦞHeap(2016a,b,c)Ⲵ ᣕ䚃ˈवᤜ㖾ഭ൘޵Ⲵཊњഭᇦҏࠪ⧠Ҷ㥹⭈㟖ᣇᙗᵲ㥹Ⲵᣕ੺ˈᴹ17⿽㥹⭈㟖 ᣇᙗᵲ㥹൘㖾ഭⲴ38њᐎᴹᣕ੺Ǆ

㲭❦ԫօа⿽䲔㥹ࡲ䮯ᵏᓄ⭘ᴰ㓸䜭Պሬ㠤ᵲ㥹ᣇᙗӗ⭏ˈնᱟᵲ㥹ᣇᙗӗ ⭏ҏᱟਟԕ䙊䗷аӋ᧚ᯭᔦ㕃ᒦ᧗ࡦⲴˈ⢩࡛ᱟᖃ䘉Ӌ᧚ᯭоާᴹн਼֌⭘ᯩᔿ Ⲵཊ⿽н਼䲔㥹ࡲ༽ਸ֯⭘ᰦˈᴤ㜭བྷབྷ䱽վᣇᙗἽṚᆈ⍫৺䘋а↕㑱㹽Ⲵਟ㜭Ǆ ˖᚟ؑޣԕлѪᵲ㥹ᣇᙗⲴаӋ⴨

ᵲ㥹ᱟᖡ૽֌⢙ӗ䟿ᴰ䟽㾱Ⲵഐ㍐ѻаˈਟሬ㠤ѕ䟽Ⲵӗ䟿ᦏཡ઼㓿⍾ᆖᖡ ૽˄Ross and Lembi, 1985; Aref and Pike, 1998; Dalley et al., 2001˅Ǆഐ↔ˈᵲ㥹䱢䲔ᱟߌъ⭏ӗѝ䶎ᑨ䟽㾱Ⲵа䜘࠶Ǆⴞࡽᑨ⭘Ⲵ䲔㥹ᯩ⌅ѫ㾱ᴹഋ⿽˖㙅֌᧚ ᯭǃ⢙⨶䲔㥹ǃॆᆖ䲔㥹઼⭏⢙䲔㥹˄Knezevic and Cassman, 2003, HRAC, 2016˅˖

ᓧ≤ᒣ઼ᣁࡦᵲ㥹⭏䮯㘼䟷ਆⲴњ㌫ࡇ㙅ڕ⢙㙅֌᧚ᯭ䲔㥹ᱟѪᨀ儈֌ - ֌ᯩ⌅ᙫ઼ˈवᤜ䘲ᖃ♼Ⓓǃᯭ㛕ǃ䘹ᤙ䘲ᓄᓖྭⲴ૱⿽ǃ䘲ᇌⲴ᫝⿽䰤 ⢙ᣔㅹüüަⴞⲴ䜭ᱟѪҶᑞࣙ֌؍⢙䐍઼᫝⿽ᇶᓖǃ䟷⭘䖞֌઼㾶൏֌ 䮯࣯䎵䗷ᵲ㥹ᡆᣁࡦᵲ㥹⭏䮯Ǆ

䲔㥹वᤜ֯⭘⢙⨶ᯩ⌅ᒢᢠᵲ㥹⭏䮯ˈवᤜᵪỠ㘫㙅ǃ࠸ࢢˈᡆ᡻ࣘ⨶⢙ - 䲔㥹Ǆ

ᆖ䲔㥹वᤜ֯⭘䲔㥹ࡲ᧗ࡦᵲ㥹Ǆॆ -

⭏⢙䲔㥹वᤜ֯⭘ᰶ㲛ǃᗞ⭏⢙઼伏㥹ࣘ⢙ᶕ᧗ࡦᵲ㥹Ǆн䗷བྷ䉶ǃ⦹㊣ - ỹ㣡ㅹаᒤ⭏֌⢙Ⲵ⭏ӗѝᖸቁ֯⭘⭏⢙䲔㥹᧚ᯭǄ઼

ᡰᴹഋ⿽ᯩ⌅䜭㜭ཏӾаᇊ〻ᓖкᴹ᭸䱢⋫ᵲ㥹ˈ൘ᇎ䱵᫽֌ѝ䙊ᑨ㓴ਸ ⭘Ǆֻྲˈ䲔㥹ࡲᑨᑨо㙅֌᧚ᯭа䎧֯⭘ˈ䇨ཊᛵߥлˈ㘫㙅઼⭏⢙᧚ᯭҏ֯ ˈ⌅㻛䇔Ѫᱟ֯⭘䲔㥹ࡲㅹѫ㾱䲔㥹᧚ᯭⲴ䖵ࣙ᡻⇥˄Beckie, 2006˅Ǆॆᆖᯩ ࡛ᱟ䲔㥹ࡲо㙀䲔㥹ࡲ֌⢙䝽ਸ֯⭘ˈਟѪߌ≁ᨀ׋ᴹ᭸Ⲵᵲ㥹᧗ࡦ᡻⇥ˈ䘹⢩ ᤙᙗൠ৫䲔ᵲ㥹ˈ㘼䇙֌⢙⭏䮯ᴤ࣐ᰪⴋ˄Knezevic and Cassman, 2003˅Ǆᦞՠ ㇇ˈ൘௧ᯭ䲔㥹ࡲⲴᶑԦлˈ⭡ᵲ㥹ᕅ䎧Ⲵӗ䟿ᦏཡӾ1%㠣20%нㅹˈ㘼ྲ᷌

VII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 н௧ᯭ䲔㥹ࡲˈӗ䟿ᦏཡࡉՊ໎࣐㠣10㠣75%˄Dalley et al., 2001, Dille et al., 2015, Dille et al., 2016˅Ǆ

⭘䲔㥹ࡲሩᵲ㥹䘋㹼᧗ࡦ㜭ཏᴹ᭸Ⲵ߿ቁӗ䟿ᦏཡˈѪᒯབྷߌ≁ᑖᶕҶ֯ ਟ㿲Ⲵ᭦⳺Ǆ֯⭘䲔㥹ࡲሩᵲ㥹䘋㹼᧗ࡦҏ߿ቁҶ⭘Ҿ㘫㙅ⲴӪᐕǃ⟳ᯉ઼ᵪỠ ,ᤱර㙅֌ൠ४ᒯ⌋䟷⭘˄Fernandez-Cornejo and McBride؍䇮༷ˈഐ↔㻛≤൏ ᤱර؍Gianessi, 2005; Gianessi and Reigner, 2006˅Ǆྲ᷌⋑ᴹ䲔㥹ࡲˈ≤൏ ;2002 ,ᤱⲴⴞḷ˄CTIC؍㲰઼໎ᕪ≤൏ץ㙅֌ሶᰐ⌅ᇎ⧠ˈҏቡᰐ⌅ᇎ⧠߿ቁ൏༔ 2016˅Ǆ䲔㥹ࡲᱟਟᤱ㔝ߌъѝнਟᡆ㕪Ⲵ䟽㾱㓴ᡀ䜘࠶˄Knezevic and Cassman, 2003˅Ǆᵲ㥹㇑⨶઼ᣇᙗᵲ㥹Ⲵ⋫⨶ҏᱟਟᤱ㔝ߌъᇎ䐥ѝ䶎ᑨ䟽㾱Ⲵ㓴ᡀ䜘࠶Ǆ ᵲ㥹ᣇᙗਟᇊѹѪḀ⿽䲔㥹ࡲⴞḷᵲ㥹ѝⲴḀа㗔փ㧧ᗇҶ㙀ਇ䘉⿽䲔㥹 ࡲⲴ㜭࣋ˈ௧ᯭ䈕䲔㥹ࡲሩ⢩ᇊ㗔փᵲ㥹н޽ᴹ᭸˄WSSA, 2011˗HRAC, 2016˅Ǆ ᵲ㥹Ⲵ䲔㥹ࡲᣇᙗᒦ䶎ᯠޤ䰞仈ˈᆳᱟа⿽㠚❦ਁ⭏Ⲵ䗷〻ǄἽ⢙㗔փਟ㜭Պᆈ ൘ḀӋቁᮠњփˈഐㅹսสഐᐞᔲ㘼ሩ㠤↫ර䲔㥹ࡲާᴹཙ❦㙀ਇᙗˈสҾ㠚❦ 䘹ᤙ⨶䇪ˈ௧ᯭ䲔㥹ࡲਾˈ᭿ᝏἽṚ㗔փ㻛䲔㥹ࡲᵰ↫ˈӵᴹ䘉Ӌቁᮠ㙀ਇරњ փᆈ⍫лᶕǄྲ᷌৽༽֯⭘਼а⿽䲔㥹ࡲ㘼н࣐ԕަԆᵲ㥹᧗ࡦ᧚ᯭ˄ྲ֯⭘֌ ˈ˅⭘ᵪࡦн਼ⲴަԆॆᆖᯩᔿ䲔㥹ˈᡆ֯⭘ᵪỠ䲔㥹ǃ㘫㙅䲔㥹ǃᡆ⭏⢙䲔㥹 ԕᰦᰕˈ䘉Ӌཙ❦ᩪᑖᴹᣇᙗสഐⲴቁᮠњփՊ䙀⑀ਁኅᡀѪѫሬ㗔փٷ ˄Beckie and Reboud, 2009; WSSA, 2011; HRAC, 2016˅Ǆሩԫօа⿽䲔㥹ࡲᶕ䈤ˈ ৽༽䮯ᵏঅа֯⭘䜭ᴹਟ㜭ӗ⭏ᣇᙗᵲ㥹㗔փˈަᣇᙗ╄ॆⲴᵪࡦᱟањ༽ᵲⲴ 䗷〻ˈн䟷⭘ަԆᵲ㥹⋫⨶ᶑԦлˈ⭡ᣇᙗㅹսสഐਁ⭏仁⦷ǃᣇᙗᵪࡦǃᱮᙗ /䳀ᙗᣇᙗㅹսสഐǃᣇᙗሿ⿽Ⲵ⴨ሩ䘲ਸᓖԕ৺䲔㥹ࡲⲴ௧ᯭ仁⦷઼ᤱ㔝ᴹ᭸ ᰦ䮯ㅹഐ㍐㔬ਸߣᇊ˄Jasieniuk et al., 1996; Beckie and Reboud, 2009; Sammons et al., 2007˅Ǆሩн਼䲔㥹ࡲᶕ䈤ˈަӗ⭏ᣇᙗᵲ㥹Ⲵਟ㜭ᙗҏнቭ⴨਼ˈḀӋ䲔㥹ࡲ˄ྲALS઼ACCase㊫˅оަԆ䲔㥹ࡲ˄ྲ⭏䮯㍐ǃ㥹⭈㟖ǃ㥹䬥㟖઼҉䞠㤟㜪㊫˅⴨∄ˈࠪ⧠ᣇᙗᵲ㥹Ⲵ䙏ᓖᴤᘛǄ䲔㥹ࡲᣇᙗⲴ䰞仈ҏнᱟ㖾ഭᡰ⢩ᴹ Ⲵ⧠䊑ˈޘ⨳㤳ത޵ሩḀӋ䲔㥹ࡲᴹ㙀ਇᙗⲴᵲ㥹઼ᣇᙗᵲ㥹㓖ᴹ250ཊ⿽ˈ࠶ ᐳҾ66њഭᇦ˄Heap, 2016a; b˅Ǆлമᡰ⽪Ѫޘ⨳㤳ത޵⺞䇔Ⲵн਼䲔㥹ࡲᇦ᯿ ᣇᙗᵲ㥹⿽㊫ᮠⴞਈॆⲴᴢ㓯ˈн਼仌㢢ԓ㺘н਼Ⲵ䲔㥹ࡲᇦ᯿ˈᯌ⦷ԓ㺘ᣇᙗ ᵲ㥹ӗ⭏Ⲵ䙏ᓖǄӾлമਟԕⴻࠪˈн਼䲔㥹ࡲᇦ᯿ᣇᙗᵲ㥹ӗ⭏Ⲵ䙏ᓖн਼Ǆ 䘁ᒤᶕˈჂփᶌ᫠ࠪањᯠ䇽Ā䎵㓗ᵲ㥹āˈ䘉њ䇽ަᇎᒦ⋑ᴹ、ᆖⲴᇊ ѹˈնᆳᑨᑨ㻛⭘ᶕᤷԓ䛓Ӌ⭡Ҿ৽༽֯⭘Ḁ⿽䲔㥹ࡲˈ൘ᤱ㔝঻࣋лӗ⭏ᣇᙗ Ⲵᵲ㥹ˈ∄ྲᣇᙗ⭏⢙රᡀѪߌ⭠ѝᑨ㿱ᵲ㥹Ⲵ⧠䊑˄WSSAˈ2016˅Ǆഐ↔ˈ Āᣇᙗᵲ㥹āǄྲࡽᡰ䘠ˈ䲔㥹ࡲᣇᙗ䰞仈ᒦڊᡰ䉃Ā䎵㓗ᵲ㥹āᇎ䱵кᓄ䈕ਛ 䶎ᯠޤһ⢙ˈҏᒦ䶎Ḁ⿽ᵲ㥹⢩ᴹⲴㄎҹᙗ⢩ᖱǄ㲭❦൘䗷৫Ⲵॱࠐᒤѝˈ⭡Ҿ 䎺ᶕ䎺ཊⲴߌ≁ӵӵ׍䎆Ҿঅа֌⭘ᵪࡦⲴᵲ㥹⋫⨶᡻⇥ˈᡁԜⴻࡠ䎺ᶕ䎺ཊⲴ ߌ⭠ᔰ࿻ࠪ⧠ᣇᙗᵲ㥹䰞仈ˈն䘉⿽ᣇᙗᵲ㥹䰞仈ᒦ䶎ӵ䲀Ҿቁᮠ䲔㥹ࡲˈᇎ䱵 кˈ൘䗷৫40ᒤѝˈᖸཊ䲔㥹ࡲ䜭ᴹ⴨ᓄⲴᣇᙗᵲ㥹㻛ᣕ䚃˄ྲлമᡰ⽪˅ˈḀ Ӌᵲ㥹⭊㠣䘋ॆࠪሩє⿽ᡆє⿽ԕк䲔㥹ࡲⲴᣇᙗ˄WSSAˈ2016˅Ǆ

VIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 н਼䲔㥹ࡲӗ⭏ᣇᙗᵲ㥹Ⲵᮠ䟿䲿ᰦ䰤ਈॆᴢ㓯 ྲ᷌н㜭⭘ަԆ㊫රⲴ䲔㥹ࡲᡆ䟷⭘ަԆ᡻⇥ᶕ᧗ࡦᣇᙗᵲ㥹㗔փˈ䲔㥹ࡲ ᣇᙗᵲ㥹ᖸᴹਟ㜭ՊᡀѪᖡ૽ߌъ⭏ӗѝⲴ䲀ࡦᙗഐ㍐˄Norsworthy et al., 2012˅ˈ նⴞࡽ䘈⋑ᴹԫօа⿽䲔㥹ࡲࠪ⧠Ҷ䘉Ѹѕ䟽Ⲵ䰞仈Ǆ↔ཆˈ⹄ਁ㘵ǃ᭯ᓌ઼⴨ ޣ、⹄ᵪᶴҏޡ਼ࣚ࣋ˈࡦᇊࠪҶаӋ㢟ྭⲴᵲ㥹䱢⋫᧚ᯭᶕᑞࣙᔦ㕃ᵲ㥹ᣇᙗ Ⲵӗ⭏ˈ਴䜘䰘ҏ൘〟ᶱ᧘㹼䘉Ӌᵲ㥹䱢⋫᧚ᯭ˄Norsworthy et al., 2012; WSSA, HRAC, 2016˅Ǆᴰ֣Ⲵᵲ㥹䱢⋫᧚ᯭवᤜ֌⢙䖞֌ǃ䘲ᖃ♼Ⓓǃ䘲ᇌ㹼䐍 ;2015 ઼᫝⿽ᇶᓖㅹ㙅֌᧚ᯭˈԕ৺㘫㙅ǃ⭠䰤㿲ሏ઼᡻ࣘ䲔㥹ㅹᵪỠ᧚ᯭǄ䶒ሩ∄䖳亭പⲴᵲ㥹ᰦˈ䘉Ӌᵲ㥹䱢⋫᧚ᯭ䘈䴰㾱оᴹ᭸ᡀ࠶н਼Ⲵཊ㊫䲔㥹ࡲ㔃ਸ֯⭘Ǆ н਼֌⭘ᵪࡦⲴ䲔㥹ࡲԕ᧘㦀ࡲ䟿਼ᰦǃ亪ᒿǃᡆ䳄ᒤ֯⭘ˈ䘉Ӌ㓴ਸਟᶱབྷൠ ߿ቁᣇᙗᵲ㥹ӗ⭏઼޽⭏Ⲵਟ㜭ᙗ˄Norsworthy et al., 2012˅Ǆ 㲭❦Ḁ⿽䲔㥹ࡲⲴ֯⭘ᴰ㓸䜭ᴹਟ㜭Պӗ⭏ᣇᙗᵲ㥹ˈն䘉⿽ᣇᙗⲴࠪ⧠㜭 ཏ䙊䗷㢟ྭⲴ䱢⋫᧚ᯭǃ⹄ウ઼ᮉ㛢㘼㻛ᔦ㕃ǃ᧗ࡦ઼㇑⨶Ǆ䲔㥹ࡲӽᱟⴞࡽᴰ ѫ㾱Ⲵᵲ㥹䱢⋫᡻⇥ˈ֯⭘֌⭘ᵪࡦн਼㘼䲔㥹䉡ᴹ䟽ਐⲴ䲔㥹ࡲᴹࣙҾ߿㕃ᵲ 㥹ᣇᙗⲴӗ⭏˄Beckie, 2006; HRAC, 2016˅Ǆ∄ྲˈྲ᷌֌⢙ਟ਼ᰦ㙀ਇཊ⿽䲔㥹ࡲˈণ֯⭠䰤ᵲ㥹ሩަѝḀа㊫䲔㥹ࡲӗ⭏Ҷᣇˈ䈕ᵲ㥹ҏਟԕ㻛ަԆ㊫䲔㥹 ࡲᵰ↫Ǆഐ↔ˈᣇཊ⿽н਼֌⭘ᵪࡦ䲔㥹ࡲⲴ֌⢙ᴹࣙҾ൘ߌ≁Ⲵᵲ㥹䱢⋫փ㌫ ѝᕅޕᯠⲴ䲔㥹ࡲˈӾ㘼ᴹਟ㜭߿㕃ᵲ㥹ᣇᙗⲴ䘋а↕ਁኅǄ

IX ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⴞࡽᐢ㓿ᴹаӋሩཊ⿽䲔㥹ࡲާᴹᣇᙗⲴӗ૱᧘ੁᐲ൪ˈѪߌ≁ᨀ׋ᴤཊᵲ 㥹䱢⋫ᯩ⌅ˈԕਟᤱ㔝ਁኅⲴᯩᔿᕪॆᵲ㥹㇑⨶᧚ᯭ˄USDA-APHIS, 2014; 2015a; ᤱ⧠ᴹ؍b and 2016˅Ǆᵲ㥹㇑⨶㌫㔏ᡀ࣏о੖׍䎆Ҿਟ⭘䲔㥹ᯩᔿⲴཊṧᙗǄ 䲔㥹ࡲ˄ྲ㥹⭈㟖઼㥹䬥㟖˅Ⲵᴹ᭸ᙗˈ਼ᰦнᯝᕅޕᢰᵟࡋᯠӗ૱˄ྲᣇ哖㥹 ߌ≁ᤕᴹ䏣ཏᕪབྷⲴᐕާᶕ⋫⨶ᵲ㥹ᒦ䙊䗷ཊṧॆⲴᵲ㥹䱢⋫᡻؍⺞⭿֌⢙˅ਟ ⇥ᔦ㕃ᵲ㥹ᣇᙗⲴӗ⭏Ǆ

৲㘳᮷⥞: 1. Aref, S. and D.R. Pike. 1998. Midwest farmers’ perceptions of crop pest infestations. Agronomy Journal 90:819-825. 2. Beckie, H.J. 2006. Herbicide-resistant weeds: Management tactics and practices. Weed Technology 20: 793-814. 3. Beckie, H.J., and X. Reboud. 2009. Selecting for weed resistance: Herbicide rotation and mixture. Weed Technology 23:363-370. 4. CTIC. 2016. Top 10 conservation tillage benefits: Conservation for agriculture's future. 5. Conservation Technology Information Center, West Lafayette, Indiana. 6. http://www.ctic.purdue.edu/resourcedisplay/293/ [Accessed September, 2016]. 7. Dalley, C.D., K.A. Renner, and J.J. Kells. 2001. Weed competition in Roundup Ready soybean and corn. Michigan Soybean Promotion Committee, Corn Marketing Program of Michigan, East Lansing, Michigan. 8. Dille, J.A., Sikkema, P.H., Everman, W.J., Davis, V.M. and I.C. Burke. 2015. Perspecitves on corn yield losses due to weeds in North America. Weed Science Society of America., Champaign, Illinois. http://wssa.net/wp-content/uploads/WSSA-2015-Corn-Yield-Loss-poster-updated-calc.p df [Accessed September, 2016] 9. Dille, J.A., Sikkema, P.H., Everman, W.J., Davis, V.M. and I.C. Burke. 2016. Perspecitves on soybean yield losses due to weeds in North America. Weed Science Society of America., Champaign, Illinois. http://wssa.net/wp-content/uploads/WSSA-2016-Soybean-Yield-Loss-poster.pdf [Accessed September, 2016] 10. Fernandez-Cornejo, J. and W.D. McBride. 2002. Adoption of bioengineered crops. U.S. Department of Agriculture, Washington, D.C. http://www.ers.usda.gov/publications/aer810/ [Accessed September, 2016] 11. Gianessi, L.P. 2005. Economic and herbicide use impacts of glyphosate-resistant crops. Pest Management Science 61: 241-245. 12. Gianessi, L. and N. Reigner. 2006. The value of herbicides in U.S. crop production: 2005 update. CropLife Foundation, Washington, D.C. 13. Heap, I. 2014. Global perspectives of herbicide-resistant weeds. Pest Management Science 70: 1306-1315. 14. Heap, I. 2016a.The International Survey of Herbicide Resistant Weeds: herbicide resistant weeds by species and site of action. WeedScience.org, Corvallis, Oregon. http://www.weedscience.org/Summary/SpeciesbySOATable.aspx [Accessed September, 2016]. 15. Heap, I. 2016b.The International Survey of Herbicide Resistant Weeds: Herbicide resistant weeds by country and site of action. WeedScience.org, Corvallis, Oregon. http://www.weedscience.org/Summary/CountrySummary.aspx [Accessed September, 2016]. 16. Heap, I. 2016c. The International Survey of Herbicide Resistant Weeds: Weeds Resistant to EPSP synthase inhibitors. WeedScience.org, Corvallis, Oregon.

X ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 http://www.weedscience.org/Summary/MOA.aspx [Accessed February, 2017]. 17. HRAC. 2016. Guideline to the management of herbicide resistance. Herbicide Resistance Action Committee. [Accessed September, 2016] http://hrac.tsstaging.com/files/Management-of-Herbicide-Resistance.pdf 18. Jasieniuk, M., A.L. Brûlé-Babel, and I.N. Morrison. 1996. The evolution and genetics of herbicide resistance in weeds. Weed Science 44:176-193. 19. Knezevic, S.Z. and K.G. Cassman. 2003. Use of herbicide-tolerant crops as a component of an integrated weed management program. Crop Management Online, St. Paul, Minnesota. 20. Norsworthy, J.K., S.M. Ward, D.R. Shaw, R.S. Llewellyn, R.L. Nichols, T.M. Webster, K.W. Bradley, G. Frisvold, S. B. Powles, N. R. Burgos, W.W. Witt, and M. Barrett. 2012. Reducing the risks of herbicide resistance: Best management practices and recommendations. Weed Science: Special Issue: 31-62. 21. Ross, M.A. and C.A. Lembi. 1985. Applied Weed Science. Burgess Publishing Co., Minneapolis. 22. Sammons, R.D., D.C. Heering, N. DiNicola, H. Glick, and G.A. Elmore. 2007. Sustainability and stewardship of glyphosate and glyphosate-resistant crops. Weed Technology 21:347-354. 23. USDA-APHIS. 2014. Determination of Nonregulated Status for Dow AgroSciences’ DAS-40278-9 Corn. U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Washington, D.C. 24. USDA-APHIS. 2015a. Determination of Nonregulated Status for Monsanto Company MON 87708 Soybean. U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Washington, D.C. 25. USDA-APHIS. 2015b. Determination of Nonregulated Status for Monsanto Company MON 88701 Cotton. U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Washington, D.C. 26. USDA-APHIS. 2016. Determination of Nonregulated Status for Monsanto MON 87419 Maize. U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Washington, D.C. 27. WSSA. 2011. Herbicide Resitance Management Lesson 3. Weed Science Society of America., Champaign, Illinois. http://wssa.net/wp-content/uploads/resistancemodules/three/index.htm 28. WSSA. 2015. WSSA lesson module: Herbicide resistant weeds. Weed Science Society of America, Champaign, Illinois http://wssa.net/wpcontent/uploads/resistancemodules/five/index.htm 29. WSSA. 2016. WSSA Fact Sheet: Dispelling Common Misconceptions about Superweeds. Public Awareness Committee of the Weed Science Society of America., Champaign, Illinois. http://wssa.net/wp-content/uploads/WSSA-Weed-Science-Definitions.pdf

XI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䰞仈 2˖ ⦹㊣㣡㊹⍫࣋Ⲵ⍻ᇊᯩ⌅ᰐ⌅⺞ᇊ⦹㊣ᮓ㊹ਾн਼ᰦᵏⲴ㣡㊹⍫࣋ˈ ф㕪ቁާփⲴỰ⍻ᮠᦞ˗ 䈤᰾˖ MON 87427 ࡙⭘Ҷ⢩ᇊⲴ੟ࣘᆀ-޵ਜ਼ᆀ㓴ਸˈഐ↔䳴ᙗಘᇈн㜭㙀ਇ 㥹⭈㟖Ǆ൘⦹㊣ᵲӔ⿽ࡦ⿽䗷〻ѝˈਟԕ⭘ਜ਼ᴹ MON 87427 䖜ॆփⲴ⦹㊣㠚Ӕ ㌫֌⇽ᵜˈُࣙҾ㥹⭈㟖䲔㥹ࡲⲴ௧ᯭӗ⭏䳴ᙗн㛢ˈݽ৫Ӫᐕᣭ䳴Ⲵ↕僔ˈབྷ བྷ㢲㓖Ҷࡦ⿽ᡀᵜǄ ൘ӗ૱ᔰਁѻࡍˈᆏኡ䜭ޜਨሩ MON 87427 䘋㹼Ҷवᤜ㣡㊹⍫࣋൘޵Ⲵޘ 䶒⧟ຳᆹޘᙗ䇴ԧǄ⧟ຳᆹޘ䇴ԧⲴ㔃᷌䇱ᇎˈоᑨ㿴⦹㊣⴨∄ˈMON 87427 ⦹㊣䖜ਈѪᴹᇣ⭏⢙઼ᵲ㥹ॆⲴ▌࣯ᒦ⋑ᴹ໎࣐Ǆަѝˈሩ MON 87427 ⦹㊣㣡㊹⍫࣋ⲴỰ⍻ᴰࡍ⭘ӊশኡབྷḃ㢢⌅䘋㹼ˈ䈖㓶ⲴỰ⍻ᯩ⌅৺㔃᷌㿱ᵜ⭣䈧ҖⲴ 72 亥Ǆӊশኡབྷḃ㢢Ⲵ㔃᷌㺘᰾ MON 87427 㣡㊹Ⲵ⍫઼࣋ᖒᘱоᑨ㿴⦹㊣⴨∄ 䜭⋑ᴹާᴹ⭏⢙ᆖ᜿ѹⲴᐞᔲǄ ṩᦞߌъ䜘৽侸᜿㿱ˈᆏኡ䜭ޜਨҾ 2017 ᒤࡍ൘㖾ഭ༿ေཧ⿽ἽҶਜ਼ᴹ MON 87427 䖜ॆփⲴ⦹㊣ᵲӔ⿽઼нਜ਼䈕䖜ॆփⲴᑨ㿴ᵲӔ⿽ሩ➗ˈ਼ᰦ䘋㹼 ⍻䈅Ⲵ䘈ᴹ 5 њ৲➗૱⿽ˈ⭘Ҿᗇࡠ㣡㊹⍫࣋ਈॆⲴཙ❦ਈᔲ㤳തǄ䈅傼䟷⭘ᆼ ޘ䲿ᵪ४㓴䇮䇑ˈ਴ 4 њ䟽༽Ǆ䈅傼⭠਴ሿ४ἽṚᔰ࿻ᮓ㊹ਾˈ䘹ᤙ൘ѝॸѻࡽ 䟷䳶㣡㊹ṧ૱ˈ㣡㊹䟷䳶ᰦ⑙ᓖᒣ൷Ѫ 21.7°CǄሶ਼аሿ४н਼ἽṚк᭦䳶Ⲵ㣡㊹␧ਸਾ⭘Ҿ㣡㊹㨼ਁ䈅傼ˈሶ㣡㊹㖞Ҿ㨼ਁษޫสࡽҾᇔ⑙˄~25°C˅ᆈ᭮ˈ ᇔ⑙ᆈ᭮ᰦ䰤࠶࡛Ѫ 0ǃ2ǃ3.5 ઼ 5 ሿᰦǄ 㣡㊹㨼ਁ䈅傼ᡰ⭘ษޫสѪՈॆⲴ㚊҉Ҽ䞷˄PEG˅Ѫส⹰Ⲵ⭏䮯ӻ䍘 ˄Subbaiah, 1984; Zhang et al., 1997; Tandon et al., 1999˅ˈަѝਜ਼ᴹ 10%㭇㌆ǃ 6% PEGǃ0.6%Ἵ⢙⩬㜲ǃ0.005%⺬䞨ǃ0.1%≟ॆ䫉઼ 0.001%⼧䞨䫮Ǆሶ㣡㊹㖞 Ҿษޫ⳯ѻਾ޽൘ᇔ⑙᭮㖞㠣ቁ 2 ሿᰦਾ䘋㹼㣡㊹㨼ਁ䇑ᮠǄ ⭘ AmScope Simul-Focal йⴞᱮᗞ䮌˄60×᭮བྷ˅䘋㹼㣡㊹㨼ਁ䇑ᮠˈᖃ㣡㊹㇑䮯ᓖı㣡㊹ⴤᖴᰦ㿶Ѫ㨼ਁ˄Kakani et al., 2002˅Ǆ⇿њᰦ䰤⛩Ӿ⇿ањሿ ४᭦䳶Ⲵ㣡㊹㠣ቁ䇑ᮠ 125 ㋂ˈ㨼ਁ⦷⭡㨼ਁⲴ㣡㊹ᮠⴞ䲔ԕ䇑ᮠⲴޘ䜘㣡㊹ᮠ ⴞਾ҈ԕ 100 ᗇࡠǄᓄ⭘ SAS PROC MIXED (SAS, 2012)㔏䇑ᆖ䖟Ԧሩ㨼ਁ⦷ᮠ ᦞ䘋㹼 ANOVA ࠶᷀Ǆ⭘ᱮ㪇ᙗ≤ᒣѪ 5%Ⲵ t-test ሩ⇿њᰦ䰤⛩ MON 87427 ઼ ᑨ㿴ሩ➗൷䘋㹼ᡀሩ∄䖳Ǆ䈅傼㔃᷌㿱㺘 1 ઼㺘 2Ǆ ⭡Ҿਇ䚇Ր઼⧟ຳㅹཊ⿽ഐ㍐Ⲵᖡ૽˄Fonseca and Westgate, 2005; Nielsen, 2010˅ˈа㡜㘼䀰㣡㊹㨼ਁ䈅傼㔃᷌Ⲵਈᔲ㤳തᖸབྷˈ㨼ਁ⦷㤳തਟӾ 13%ࡠ 75%нㅹ˄Fonseca and Westgate, 2005˅Ǆо᮷⥞ᣕ䚃а㠤ˈᵜ⹄ウѝн਼৲㘳 ૱⿽н਼ᰦ䰤⛩Ⲵ㣡㊹㨼ਁ⦷Ⲵਈᔲ㤳തҏ䖳བྷ˄㺘 2˅Ǆ㔏䇑ᆖ࠶᷀㔃᷌㺘᰾ˈ ൘⇿њᰦ䰤⛩ˈMON 87427 оᑨ㿴ሩ➗Ⲵ㨼ਁ⦷൷ᰐ㔏䇑ᆖᱮ㪇ᙗᐞᔲ˄㺘 1˅ˈ ф MON 87427 н਼ᰦ䰤⛩㣡㊹㨼ਁ⦷Ⲵ൷٬սҾ৲㘳૱⿽Ⲵ㠚❦ਈᔲ㤳ത޵Ǆ ᵜ⹄ウⲴ㔃᷌оѻࡽӊশኡབྷ⌅㣡㊹ḃ㢢Ⲵ㔃᷌а㠤ˈ൷᭟ᤱ MON 87427 оᑨ㿴ሩ➗㣡㊹⍫࣋⴨ᖃⲴ㔃䇪Ǆ↔ཆˈ2008 ᒤ㖾ഭ⭠䰤䈅傼઼ 2014 ᒤ൘ਹ᷇ ⴱ䘋㹼Ⲵഭ޵⧟ຳᆹޘỰ⍻Ⲵ㔃᷌൷㺘᰾ MON 87427 ઼ᑨ㿴ሩ➗൘㺘ර৺ߌ㢪

XII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᙗ⣦઼о⧟ຳӂ֌ㅹᯩ䶒൷⴨ᖃˈᵚ㿲ሏࡠԫօᴹ⭏⢙ᆖ᜿ѹⲴᐞᔲǄ㺘᰾оᑨ 㿴ሩ➗⴨∄ˈMON 87427 䖜ਈѪᴹᇣ⭏⢙ᡆᵲ㥹Ⲵ▌࣯ᒦ⋑ᴹ໎࣐Ǆ↔ཆˈਜ਼ ᴹ MON 87427 Ⲵ⦹㊣㊭㋂ӗ૱ӵݱ䇨֌Ѫ࣐ᐕ৏ᯉ䘋ਓࡠѝഭˈнՊ൘ѝഭຳ ޵䘋㹼⿽Ἵˈഐ↔ MON 87427 ἽṚ৺ަᡰӗ㣡㊹Ⲵ⧟ຳ᳤䵢䟿ᶱվǄ

˅SD±㺘 1. MON 87427 ઼ᑨ㿴ሩ➗㣡㊹⍫࣋Ⲯ࠶ᮠᙫ㔃㺘˄൷٬ T11 T22 T33 T44 ᶀᯉ ᶀᯉ㊫ර ˄%˅ ˄%˅ ˄%˅ ˄%˅ MON 87427 ⍻䈅⢙ 66.5 (3.6) 70.6 (4.4) 70.7 (2.8) 68.1 (3.1) LH198+LH287 ᑨ㿴ሩ➗ 57.5 (1.3) 63.6 (2.4) 60.6 (3.1) 59.6 (3.1) ৲㘳㤳ത 5 ৲㘳㤳ത 54.7 – 74.5 56.5 – 75.1 48.2 – 72.7 49.4 – 72.5 1 ᭦䳶ਾ・ণ䇑ᮠ˗2 ᭦䳶ਾᇔ⑙᭮㖞 2 ሿᰦ˗3 ᭦䳶ਾᇔ⑙᭮㖞 3.5 ሿᰦ˗4 ᭦䳶ਾᇔ ⑙᭮㖞 5 ሿᰦ˗5 5 њ৲㘳૱⿽Ⲵᴰབྷ٬઼ᴰሿ٬Ǆ

˅SD±㺘 2. ৲㘳૱⿽㣡㊹⍫࣋Ⲯ࠶ᮠᙫ㔃㺘˄൷٬ T11 T22 T33 T44 ᶀᯉ ᶀᯉ㊫ර ˄%˅ ˄%˅ ˄%˅ ˄%˅ Stewart S750 ৲㘳૱⿽ 57.8 (3.2) 58.7 (3.3) 52.2 (4.1) 49.4 (3.1) Stine 9628 ৲㘳૱⿽ 54.7 (5.9) 56.5 (8.0) 48.2 (6.1) 50.3 (7.1) DKC64-85 ৲㘳૱⿽ 70.8 (5.1) 75.0 (3.4) 72.6 (5.5) 66.3 (3.8) Lewis 1613 ৲㘳૱⿽ 74.5 (2.1) 68.1 (7.1) 57.4 (6.9) 56.6 (11.1) HCL654+LH244 ৲㘳૱⿽ 69.2 (1.0) 75.1 (2.6) 72.7 (3.7) 72.5 (1.1) 1 ᭦䳶ਾ・ণ䇑ᮠ˗2 ᭦䳶ਾᇔ⑙᭮㖞 2 ሿᰦ˗3 ᭦䳶ਾᇔ⑙᭮㖞 3.5 ሿᰦ˗4 ᭦䳶ਾᇔ ⑙᭮㖞 5 ሿᰦ˗5 5 њ৲㘳૱⿽Ⲵᴰབྷ٬઼ᴰሿ٬Ǆ

XIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䰞仈 3˖ 㕪ቁ䖜สഐ⦹㊣ሩ劬㊫ǃ⿭㊫ᖡ૽Ⲵ∂⨶ᆖ䇴ԧ䍴ᯉǄ 䈤᰾˖൘ӗ૱ᔰਁѻࡍˈᆏኡ䜭ޜਨԕ MON 87427 ⦹㊣㊭㋂Ѫ侢ᯉᡀ࠶ˈ࠶࡛ ሩ劬㊫઼⿭㊫䘋㹼Ҷ㩕ޫᆖ䇴ԧˈᒦоᑨ㿴ሩ➗⦹㊣ᡆ৲➗⦹㊣㊭㋂ࡦᡀⲴ侢ᯉ லޫ㔃᷌䘋㹼∄䖳Ǆ䙊䗷∄䖳␫࣐ᴹ䖜สഐ⦹㊣Ⲵ侢ᯉ઼␫࣐ᴹᑨ㿴⦹㊣Ⲵ侢ᯉ Ⲵᇎ傼ࣘ⢙൘⭏䮯઼ᆈ⍫⦷ᯩ䶒ᱟ੖ᆈ൘ᐞᔲˈӾ㘼⺞ᇊ䖜สഐ⦹㊣൘㩕ޫᆖᯩ 䶒ᱟ੖оᑨ㿴⦹㊣ᆈ൘ᐞᔲǄ⹄ウᣕ੺䈧㿱ᵜ⭣䈧䍴ᯉл޼䱴Ԧᢰᵟᣕ੺ 17 ઼ ᢰᵟᣕ੺ 18Ǆ 劬㊫லޫᇎ傼 ⭘Ҿ⍻䈅Ⲵ劬㊫Ѫᯁ⛩৹ቮ几˄Ictalurus punctatus˅ˈᱟа⿽৏ӗҾे㖾⍢ Ⲵབྷර␑≤劬㊫ˈഐާᴹ伏ᵲᙗǃ⭏䮯ᘛǃ䘲ᓄᙗᕪㅹ⢩⛩㘼ᑨᑨ㻛⭘֌㩕ޫ⹄ ウ⍻䈅⭘劬㊫Ǆ劬侢ᯉѝᡰਜ਼⦹㊣㊭㋂Ⲵ∄ֻ˄30%˅⴨ᖃҾᡆ儈Ҿᐲ୞劬侢ᯉ ѝⲴ⦹㊣ਜ਼䟿Ǆ␫࣐ᴹ䖜สഐ⦹㊣㊭㋂ǃሩ➗⦹㊣㊭㋂ᡆ৲➗⦹㊣㊭㋂Ⲵ劬侢ᯉˈ ަᙫփᡀ࠶оᐲ୞ᯁ⛩৹ቮ几劬Ⲵ侢ᯉᡀ࠶⴨լǄ侢ᯉ㓿䝽ࡦਾަ㋇㳻ⲭ䍘ਜ਼䟿㓖Ѫ 32%ˈᒦਜ਼ᴹㅹ䟿≞Ǆሩ劬侢ᯉ䘋㹼࠶᷀ˈᵚਁ⧠н਼㊫ර劬侢ᯉѻ䰤൘ᑨ 㿴㓴࠶ਜ਼䟿кᆈ൘ᱮ㪇ᐞᔲǄ

⹄ウ⭘劬䍝㠚ᇶ㣿䟼ᐎྕຎཷ⎧┙Ⲵ Osage Catfisheries ⑄൪ѝ 2010 ᒤ 7 ᴸᆥ ॆⲴ䖳བྷаネ劬ˈഐަᮠ䟿䖳བྷˈн޽ሩњփаа䘋㹼ᙗ࡛䢤ᇊǄ䘀䘱ࡠ⍻䈅ᵪᶴ ਾ䇙劬㗔൘བྷර≤㇡ѝ䘲ᓄǄ䘲ᓄᵏ䰤ˈᑨ㿴ல伏ᒦ䘋㹼≤䍘ⴁ⍻ˈ劬㗔ԕᐲ୞劬侢ᯉѪ伏ˈல伏䟿㓖Ѫ⇿ཙ 3%փ䟽Ǆ䘲ᓄйઘਾˈԫ᜿࠶ࠪа㓴劬ˈޡ䇑 800 ᶑ 劬㻛䖜〫ࡠ⍻䈅⭘Ⲵ 20 њ≤㇡ѝ䘋㹼傟ॆǄ䘲ᓄ傟ॆ䗷〻ᆼᡀਾˈ਴њ≤㇡ѝⲴ 劬᤹➗փරབྷሿ࠶㓴Ǆ⇿ 20 ᶑ⴨լփරⲴ劬˄֌Ѫ਼а㓴˅䘋㹼〠䟽ˈ❦ਾ᭮ എ৏≤㇡Ǆ਴≤㇡ѻ䰤劬㗔њփⲴ䎧࿻ᒣ൷փ䟽Ѫ 7.0-7.3 gǄ20 њ⍻䈅⭘≤㇡㻛䲿ᵪ࠶Ѫ 5 њ侢ல㓴ˈ⇿㓴 4 њ≤㇡ˈ⇿њ≤㇡ѝ 40 ᶑ劬Ǆ ᡰᴹ劬侢ᯉࡦᡀਟл⊹Ⲵ仇㋂⣦ˈӾㅜ 0 ཙᔰ࿻ˈ䘎㔝 8 ઘ⇿ཙੁ劬㗔ᣅல 䇱 40 min؍ᤱа㠤Ǆᣅ᭮侢ᯉⲴ䗷〻؍ᤷᇊⲴ⍻䈅侢ᯉˈ⇿ཙⲴᣅலᰦ䰤ቭ䟿䇱ᡰᴹⲴ劬䜭㜭൘ 40؍޵லᆼᡰᴹ≤㇡䟼Ⲵ劬ˈ⇿⅑侢ᯉᣅலⲴ䟿઼ᰦ䰤䰤䳄 min Ⲵᣅல䰤䳄޵䗮ࡠ価伏⣦ᘱǄ൘⹄ウᔰ࿻ࡽаཙǃ⹄ウㅜഋઘ઼ㅜޛઘᆼᡀ ਾ࠶࡛䘋㹼䇑ᮠ઼〠䟽Ǆ⇿ᰕ䇠ᖅ劬Ⲵ侢ᯉ⎸㙇䟿ԕ৺ᇎ傼ࣘ⢙↫ӑᛵߥǄ ሩ劬㊫Ⲵ⍻䈅㔃᷌䈧㿱ᵜ⭣䈧䍴ᯉл޼䱴Ԧᢰᵟᣕ੺ 17Ǆ⹄ウᮠᦞ㺘᰾ˈ 䈅㓴Ⲵᆈ⍫⦷Ѫ 100%ˈሩ➗㓴ѝᴹаֻ↫ӑˈᵚ㿲ሏࡠަԆ劬㗔Ⲵ↫ӑᡆᔲ⍻ ᑨ㹼ѪǄᶕⓀҾ MON 87427 ⦹㊣㊭㋂Ⲵ劬侢ᯉо᧪ᴹᑨ㿴ሩ➗⦹㊣ᡆᐲ୞⦹㊣ Ⲵ劬侢ᯉ⴨∄ˈሩᯁ⛩৹ቮ几劬Ⲵ侢ᯉ⎸㙇ǃփ䟽໎࣐ǃ侢ᯉ䖜ᦒ∄ǃᆈ⍫⦷৺ 㹼Ѫ㺘⧠⋑ᴹн㢟ᖡ૽ǄสҾ㿲ሏ㔃᷌ˈ䇔Ѫ MON 87427 ⦹㊣㊭㋂൘侢லᯁ⛩ ৹ቮ几劬ᰦˈоᑨ㿴⦹㊣㊭㋂൘㩕ޫᆖᯩ䶒ᇎ䍘ㅹ਼Ǆ ⿭㊫லޫᇎ傼 ⭘Ҿ⍻䈅Ⲵ⿭㊫Ѫ୶⭘㚹呑૱⿽˄Gallus domesticus˅ˈᱟа⿽⭏䮯䙏ᓖ䖳 ᘛⲴᑨ㿱ᇦ⿭ˈ䘲⭘Ҿ䘋㹼㩕ޫᆖ⹄ウǄ⍻䈅ࣘ⢙൘᭦ࡠᰦ൷Ѫ 1 ᰕ喴㚹呑૱⿽ Cobb × Cobb 500ˈ䳼䳴䲿ᵪǄ䟷⭘䲿ᵪᆼޘ४㓴䇮䇑ሶ༴⨶࠶䝽㠣呑ㅬǄሶ

XIV ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 MON 87427 ⦹㊣ǃᑨ㿴ሩ➗⦹㊣ԕ৺ 6 ⿽৲➗⦹㊣૱⿽Ⲵ㊭㋂࠶࡛࣐ᐕᡀ㊹ᵛˈ ԕ呑侢ᯉᴰ儈⦹㊣␫࣐ࡲ䟿␧ޕ侢ᯉѝ䘋㹼 42 ཙலޫǄާփ࠶㓴ྲл㺘ᡰ⽪˖ ༴⨶1 ⦹㊣ḷ䇶 ⇿⿽ᙗ࡛ ޜ呑ᮠⴞ ⇽呑ᮠⴞ ᙫ呑ᮠ/ ᙫ呑ᮠ/ ㅬᮠ /ㅬ2 /ㅬ2 ᙗ࡛ ༴⨶ 1 Pioneer 34P88 5 10 10 50 100 2 NK N72-G8 5 10 10 50 100 3 MON 87427 5 10 10 50 100 4 Middlekoop 4413 5 10 10 50 100 5 Garst 8450 5 10 10 50 100 6 Golden Harvest 5 10 10 50 100 H8920 7 DKC62-30 5 10 10 50 100 8 ᑨ㿴ሩ➗ 5 10 10 50 100

1 ⴤࡠᵜ⹄ウ⭏ᆈ䱦⇥㢲⇥ᆼᡀѻࡽ༴⨶ḷ䇶аⴤн⸕ᲃǄ 2 ᇎ傼ᔰ࿻ᰦ⇿ਚㅬᆀ൷ᴹ2ਚཊ։Ⲵ䳿呑ˈԕᕕ㺕ࡽ7ཙ⭡Ҿ↫ӑǃ侯↫઼〫ࠪⲴ䳿呑Ǆ ྲIII. Cа㢲ᡰ䘠ˈԫօཊ։Ⲵ䳿呑൘ㅜ7ཙ〫ࠪǄᖃ侢ᯉ䖜ॆ⦷઼փ䟽֌Ѫѫ㾱⹄ウᮠ Ǆ൘㚹呑侢லᇎ傼ѝᑨᑨਁ⭏ഐ侕侯㘼↫઼〫ࠪ䳿呑⌅ڊᦞᰦˈ䘉ᱟ䈕⹄ウᇎ傼Ⲵḷ߶ ⲴᛵߥǄ

䘎㔝லޫਾҾ 42 ᰕ喴〠䟽ˈ43-44 ᰕ喴ᰦ䟷⭘亸Ἶ㝡ս⌅༴↫ˈ䘋㹼࠶᷀Ǆ ൘ᇎ傼ㅜ 0 ཙ˄᧕᭦ᖃཙ˅઼ 42 ཙ˄⭏ӗᙗ㜭䇴ԧ䱦⇥㔃ᶏ˅᤹ㅬѪ呑〠䟽Ǆ 〠呑ㅬ᤹㓴࡛〠䟽ˈє㓴൘਼аཙ〠䟽Ǆ䳿呑֙ᡤ㗵ḷˈነᇠ࣐ᐕѻࡽ・ণ䙀а 䟽Ǆㅜ 21 ཙ઼ㅜ 42 ཙல伏ಘѝ࢙։Ⲵ侢ᯉ〠䟽ˈ呑ㅬѝⲴ侢ᯉᙫ䟿߿৫呑ㅬѝ Ⲵ侢ᯉˈ䇑㇇䟷伏䟿Ǆ䇑㇇⭏ӗᙗ㜭ᮠᦞˈᙫ㔃ㅜ 42 ཙ⇿ਚ呑Ⲵᒣ൷໎䟽Ǆᙫ 䟷伏䟿䲔ԕ䈕ㅬᆈ⍫呑Ⲵᙫ䟽䟿ˈᗇࠪㅜ 0-42 ཙⲴᒣ൷侢ᯉ:໎䟽∄Ǆᙫ䟷伏䟿䲔ԕᆈ⍫呑Ⲵ໎䟽࣐к↫ӑ⍫Ӿ䈕ㅬѝ〫ࠪⲴ呑໎䟽䇑㇇䈳ᮤ侢ᯉ:໎䟽∄ˈণ˖ ㅜ 0-42 ཙ䈳ᮤ侢ᯉ:໎䟽∄ = ㅜ 0-42 ཙ䟷伏䟿 ÷[(ㅜ 42 ཙㅬ䟽 – ㅜ 0 ཙㅬ䟽) +(ㅜ 0-42 ཙ↫ӑ/〫ࠪ呑Ⲵփ䟽 – ㅜ 0 ཙᒣ൷փ䟽)Ǆ ሩ䇠ᖅ㔃᷌䘋㹼䲿ᵪᆼޘ४㓴䇮䇑ⲴҼഐ㍐ᯩᐞ࠶᷀ˈєњഐ㍐ᤷ侢ᯉ㊫ ර઼呑Ⲵᙗ࡛Ǆާփ࠶᷀㔃᷌䈧㿱ᵜ⭣䈧䍴ᯉл޼䱴Ԧᢰᵟᣕ੺ 18Ǆ֯⭘ MON 87427ǃᑨ㿴ሩ➗ᡆ 6 њᑨ㿴৲➗⦹㊣ᵲӔ⿽㊭㋂䝽ࡦⲴ侢ᯉ䘎㔝லޫ㚹呑 ⧠ཙˈަ䓛փᙗ㜭Ⲵ⍻ᇊ٬⴨լˈণн਼ᶕⓀ⦹㊣侢ᯉሩ㚹呑Ⲵ䓛փᙗ㜭㺘 42 ⋑ᴹਁ⧠䶎亴ᵏⲴн㢟ᖡ૽Ǆ༴↫ਾቨ䀓࠶᷀㔃᷌ҏ㺘᰾ˈԕн਼ᶕⓀ⦹㊣ᵲӔ ᒣ⴨ᖃǄ≥⿽㊭㋂䝽ࡦⲴ侢ᯉ䘎㔝லޫ㚹呑 42 ཙˈަᇠᵰਾ䓛փ⍻ᇊ٬Ⲵᮠ٬ 㔬кˈ侢லਜ਼ MON 87427 侢ᯉ઼侢லਜ਼ᑨ㿴ሩ➗⦹㊣Ⲵ㚹呑䓛փᙗ㜭ǃነᇠ⦷ ᡆ㚹ᡀ࠶кнᆈ൘⭏⢙⴨ޣᐞᔲǄਜ਼ MON 87427 Ⲵ侢ᯉо࡙⭘ᑨ㿴ሩ➗ᡆ୶૱ Ǆṩᦞ䘉Ӌ䍴ᯉޘڕ➗⦹㊣㊭㋂䝽ࡦⲴ侢ᯉ൘᭟ᤱ㚹呑ᘛ䙏⭏䮯㜭࣋ᯩ䶒аṧ৲ ਟԕᗇࠪ㔃䇪ᱟ MON 87427 оᑨ㿴⦹㊣൘㩕ޫкᱟ⴨਼ⲴǄ

XV ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߌъ䜘ޣҾMON 87427ᆹޘ䇴ԧ⭣䈧Ⲵഎ༽࠭ ˄2015ᒤ12ᴸ31ᰕ˅

XVI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ҿߌъ䜘ᢩ༽ؑ࠭Ⲵ䈤᰾ޣਨޜᆏኡ䜭

䰞仈˖ᙕᙗ∂ᙗ䈅傼ᵚ᤹➗ḷ߶ǉ㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼Ǌ˄ߌъ䜘 2031 ਧޜ ੺-16-2013˅Ⲵ㾱≲ᆼᡀǄ

ᴰᯠᆼᡀⲴCP4 EPSPSᙕᙗ∂ᙗ⹄ウ⭡SmedleyㅹӪҾ2016ᒤ2ᴸᆼᡀˈ䈅 ḷ߶ޣ傼䇮䇑ѫ㾱׍ᦞEPA OPPTS 870.1100 ˈҏ┑䏣ߌъ䜘亱ਁⲴ⴨ NY-2031-16-2013Ǆ൘ᔪ・ҶἽ⢙ᶕⓀ㳻ⲭоབྷ㛐ᵶ㧼㺘䗮㌫㔏㓟ॆ㳻ⲭѻ䰤ᇎ 䍘ㅹ਼ᙗⲴส⹰к˄ᢰᵟᣕ੺5˅Ǆ⍻ᇊᗇࡠⲴབྷ㛐ᵶ㧼㺘䗮㓟ॆⲴCP4 EPSPS 㳻ⲭ㓟ᓖѪ92%ˈ㓿㓟ᓖṑ↓ਾⲴ♼㛳ⓦ⏢ѝCP4 EPSPS㳻ⲭⲴᇎ䱵⎃ᓖѪ83.9 mg/mLˈ䙊䗷㓿ਓᯩᔿঅᰕє⅑♼㛳䳼䳴਴10ਚCD-1ሿ啐ˈ⇿⅑♼㛳փ〟Ѫ33.3 mL/kgփ䟽˄䰤䳄བྷ㓖3-4ሿᰦ˅ˈԕ䗮ࡠ5000 mg/kgփ䟽Ⲵᙫࡲ䟿Ǆ਼ᰦ䘋㹼䈅傼Ⲵ䘈ᴹⓦࡲሩ➗㓴ԕ৺ԕ਼ㅹࡲ䟿ሿ⢋㹰␵㳻ⲭ˄BSA˅♼㛳Ⲵ㳻ⲭሩ➗㓴Ǆ ᡰᴹࣘ⢙൘♼㛳ࡽ൷⾱伏༴⨶ˈ♼㛳ਾ⇿ཙє⅑㿲ሏሿ啐ᱟ੖↫ӑᡆᱟ㺘⧠ࠪѝ ∂⯷⣦ˈ㿲ሏᵏѪ14ཙǄ䈖㓶ⲴѤᒺ㿲ሏᮠᦞवᤜ˖൘䲿ᵪ࠶㓴ѻᰕ˄ㅜ-2ཙ˅ 㿲ሏ1⅑ǃㅜ0ཙ♼㛳ࡽ㿲ሏ1⅑ǃㅜ0ཙ♼㛳ਾ㠣ቁ㿲ሏ2⅑˄ㅜа⅑♼㛳1ሿᰦ޵ 1⅑ˈㅜҼ⅑♼㛳ਾ㓖2ሿᰦ1⅑˅ˈѻਾ˄ㅜ1~14ཙ˅⇿ཙ㠣ቁ㿲ሏ2⅑Ǆ൘䲿ᵪ ࠶㓴ᖃཙ˄ㅜ-2ཙ˅ǃ⾱伏ࡽ˄ㅜ0ཙ˅ǃ♼㛳ࡽ˄ㅜ0ཙˈ⾱伏˅઼ㅜ7઼14ཙˈ ሩ⇿ਚሿ啐〠䟽Ǆ൘ㅜ0~7ཙ઼ㅜ7~14ཙᇊ䟿⍻ᇊ⇿ਚሿ啐Ⲵਆ伏䟿Ǆㅜ14ཙᰦ ሶᡰᴹࣘ⢙༴↫ˈ䘋㹼བྷփ䀓ࢆˈवᤜቨփ઼㚼㚹僘僬㌫㔏Ⲵ䇴ԧǃᡰᴹཆ㺘䶒 ઼ᆄਓǃ亵㞄઼བྷ㝁Ⲵཆ㺘䶒ˈԕ৺㜨㞄ǃ㞩㞄઼⳶㞄оަ⴨ޣⲴಘᇈ઼㓴㓷Ǆ

⹄ウ㔃᷌㺘᰾ˈᇎ傼ᵏ䰤⋑ᴹਁ⭏↫ӑ⧠䊑ˈҏ⋑ᴹ㿲ሏࡠоਇ䈅⢙⴨ޣ ⲴѤᒺ⯷⣦ˈᒦф൘փ䟽ǃփ䟽ਈॆǃᩴ伏䟿ᡆབྷփቨỰ㺘⧠ᯩ䶒⋑ᴹਁ⧠о CP4 EPSPS⴨ޣⲴᐞᔲǄ⭡Ҿ൘ሿ啐ᆈ⍫⦷ǃѤᒺ㺘⧠ǃփ䟽ǃփ䟽໎࣐ǃ伏⢙ ⎸㙇ᡆབྷփ⯵⨶ᆖᯩ䶒൷ᵚਁ⧠о༴⨶⴨ޣⲴн㢟৽ᓄˈഐ↔⺞ᇊCP4 EPSPS㳻 ⲭⲴᰐ᰾ᱮᦏᇣ֌⭘ࡲ䟿˄NOAEL˅㠣ቁѪ䈅傼ᴰ儈ࡲ䟿ণ5000 mg/kgփ䟽Ǆ

ᓧ⣦ߥᶑḕᣕ੺ѻҼڕṩᦞ㘏ࠔ㤡઼ᶘᲃݹ˄2006˅൘ǉѝഭት≁㩕ޫо ˄2002˅üü㟣伏о㩕ޫ㍐ᩴޕ⣦ߥǊࡇࠪⲴѝഭӪᒣ൷⎸䍩⦹㊣Ⲵ䈳ḕᛵߥˈ ѝഭӪ⇿ཙ⇿Ӫᒣ൷Ⲵ⦹㊣⎸䍩䟿Ѫ9.9 gˈṩᦞѝഭӪᒣ൷փ䟽㓖50 kgˈᣈ㇇ ᡀ⇿ཙ⇿ޜᯔփ䟽⎸䍩䟿ࡉѪ0.2 gǄ㔃ਸMON 87427⦹㊣㊭㋂ѝCP4 EPSPS㳻ⲭ Ⲵᒣ൷㺘䗮≤ᒣ˄3.6 μg/g㓴㓷勌䟽˅ˈ⭡↔ՠ㇇ѝഭӪ㗔䙊䗷㟣伏ᩴޕ XVII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 MON 87427⦹㊣㊭㋂㘼ӗ⭏ⲴCP4 EPSPS㳻ⲭ᳤䵢MOE儈䗮6.9 × 106Ǆഐ↔ˈᯠ 䘁ᆼᡀⲴሿ啐ᙕᙗ㓿ਓ♼㛳⹄ウ޽⅑䇱ᇎˈCP4 EPSPS㳻ⲭሩ䈅傼ሿ啐⋑ᴹн㢟 ᖡ૽ˈ䈕ᆹޘ♼㛳ࡲ䟿⴨ᖃҾањᲞ䙊ᡀᒤӪ⇿ཙ伏⭘㓖69੘ⲴMON 87427⦹㊣㊭㋂᡽㜭䗮ࡠⲴCP4 EPSPS㳻ⲭ᳤䵢≤ᒣǄ

㠚Ӿ1996ᒤᣇ㥹⭈㟖བྷ䉶୶ъॆ⿽Ἵԕᶕˈਜ਼ᴹCP4 EPSPS㳻ⲭⲴߌӗ૱ 伏⭘Ǆਜ਼ᴹCP4 EPSPSⲴᣇޘ伏⢙׋ᓄ䬮ѝᆈ൘ᒦ㻛Ӫ㊫઼ࣘ⢙ᆹ⨳ޘቡаⴤ൘ 㥹⭈㟖֌⢙⇿ᒤ䜭ᴹ30ཊњӗ૱൘13њഭᇦ⿽Ἵ䶒〟䎵䗷7400зޜ产˄Dill et al., 2008˅Ǆ㠚1996ᒤᣇ㥹⭈㟖֌⢙୶ъॆ⿽Ἵԕᶕˈᐢᴹ䎵䗷1000ӯཤࣘ⢙㻛侢ல ⦷ᓧ઼⭏ӗڕ⢙ਜ਼ᴹ䖜สഐ֌⢙˄བྷ䜘࠶ᱟᣇ㥹⭈㟖֌⢙˅Ⲵ侢ᯉˈ⋑ᴹᖡ૽ࣘ Ⲵᣕ੺Ǆаㇷ㓿䗷਼㹼䇴䇞ⲴޣҾ侢லਜ਼ᴹ䖜สഐ֌⢙˄वᤜᣇ㥹⭈㟖֌⢙˅侢 ᓧᖡ૽Ⲵ᮷⥞㔬䘠˄Eenennaam & Young, 2014˅ҏᤷࠪˈⴞڕᯉሩࣘ⢙⭏䮯઼ ᓧӗ⭏н࡙ᖡ૽Ǆڕࡽ䘈⋑ᴹԫօֻᆀ㺘᰾ਜ਼ᴹ䖜สഐ֌⢙侢ᯉՊሩࣘ⢙⭏䮯઼ ൘䗷৫20ᒤˈޘ⨳ᐢ㓿ᴹ488亩⅑ޣҾCP4 EPSPS㳻ⲭᆹޘᙗⲴᇑḕᡆ䇴ᇑˈᗇ ᓧ⋑ᴹн㢟ᖡ૽Ǆڕ⢙ࠪⲴ㔃䇪൷䇔ѪCP4 EPSPS㳻ⲭሩӪ㊫઼ࣘ

MON 87427⦹㊣ѝୟаᕅޕⲴᯠ㺘䗮ᱟCP4 EPSPS㳻ⲭˈоަԆᐢ㓿୶ъ ॆⲴ30ཊ⿽ᣇ㥹⭈㟖֌⢙ѝ㺘䗮ⲴCP4 EPSPS㳻ⲭᆼޘ⴨਼Ǆᆏኡ䜭ޜਨ࡙⭘ሩ བྷ㛐ᵶ㧼㺘䗮㓟ॆⲴCP4 EPSPS㳻ⲭ䘋㹼Ҷཊ⅑ሿ啐ᙕᙗਓᴽ∂ᙗ⹄ウǄ䘉Ӌ⹄ ウᒦ䶎ࠪҾሩCP4 EPSPS㳻ⲭᆹޘᙗᆈ⯁ˈ㘼ᱟࠪҾ䟽㿶ߌъ䜘уᇦ൘৽侸᜿㿱 ѝᨀࠪⲴሩнㅖਸ䈅傼ḷ߶Ⲵᙕᙗ⹄ウ∂ᙗ⹄ウᯩ⌅Ⲵ⯁㲁ǄᡰᴹⲴᙕᙗਓᴽ∂ ᓧ⋑ᴹԫօн㢟ᖡ૽Ǆڕᙗ⹄ウ㔃᷌৽༽䇱ᇎˈCP4 EPSPS㳻ⲭሩପңࣘ⢙Ⲵ

৲㘳᮷⥞˖ ᓧ⣦ߥᶑḕᣕ੺ѻҼ˄2002˅ˉ㟣伏о㩕ޫڕ㘏ࠔ㤡, ᶘᲃݹ. 2006. ѝഭት≁㩕ޫо .1 ㍐ᩴޕ⣦ߥ, pp. 21-33, Ӫ≁ছ⭏ࠪ⡸⽮. 2. ǉ䖜สഐἽ⢙ᆹޘ䇴ԧᤷইǊ˄ѝഭߌъ䜘ˈ2010˅ 3. Clive, J. 2014a. Global status of commercialized biotech/GM crops: 2014. ISAAA Brief No. 49. International Service for the Acquisition of Agri-Biotech Applications. Ithaca, NY. USA. 4. Codex Alimentarius (2009). Foods derived from modern biotechnology. Rome, Italy, Codex Alimentarius Commission, Joint FAO/WHO Food Standards Programme, Food and Agriculture Organization of the United Nations. Second Edition. 5. Eenennaam, A. L. v. and A. E. Young (2014). "Prevalence and impacts of genetically engineered feedstuffs on livestock populations." Journal of Animal Science 92(10): 4255-4278. 6. Kuiper, H. A., et al. (2013). "New EU legislation for risk assessment of GM food: no scientific justification for mandatory animal feeding trials." Plant Biotechnology Journal 11(7): 781-784.

XVIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߌъ䜘ޣҾMON 87427ᆹޘ䇴ԧ⭣䈧Ⲵഎ༽࠭ ˄2015ᒤ9ᴸ30ᰕ˅

XIX ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ҿߌъ䜘ᢩ༽ؑ࠭Ⲵ䈤᰾ޣਨޜᆏኡ䜭

䰞仈˖ᙕᙗ∂ᙗ䈅傼ᵚ᤹➗ḷ߶ǉ㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼Ǌ˄ߌъ䜘 2031 ਧޜ ੺-16-2013˅Ⲵ㾱≲ᆼᡀǄ

䈤᰾˖㠚 1996 ᒤㅜаֻ䖜สഐᣇ㥹⭈㟖བྷ䉶୶ъॆԕᶕˈCP4 EPSPS 㳻ⲭ䗴Ӻ Ⲵᯠ㺘䗮ᱟޕ⭘শਢǄMON 87427 ⦹㊣ѝୟаᕅ֯ޘѪ→ᐢᴹ䘁 20 ᒤⲴᆹ CP4 EPSPS 㳻ⲭˈоަԆᐢ㓿୶ъॆⲴ 30 ཊ⿽ᣇ㥹⭈㟖֌⢙ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭᆼޘ⴨਼Ǆ ڕ⢙㲭❦䘁 20 ᒤⲴ୶ъॆᓄ⭘䗷〻ѝᒦᵚਁ⧠ CP4 EPSPS 㳻ⲭሩӪ㊫઼ࣘ ᙗ䇴ԧ䍴ᯉˈ൘䇱ᇎབྷ㛐ᵶ㧼ޘ䶒Ⲵᆹޘᓧᴹԫօн࡙ᖡ૽ˈн䗷ˈѪҶᨀ׋ᴤ 㺘䗮㌫㔏ӗ⭏㳻ⲭо MON 87427 ⦹㊣ѝᯠ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭѻ䰤ާᴹㅹ਼ ᙗⲴส⹰кˈᆏኡ䜭ޜਨ࡙⭘ሩབྷ㛐ᵶ㧼㺘䗮㓟ॆⲴ CP4 EPSPS 㳻ⲭ䘋㹼Ҷཊ ⅑ሿ啐ᙕᙗਓᴽ∂ᙗ⹄ウǄ䘉Ӌ⹄ウᒦ䶎ࠪҾሩ CP4 EPSPS 㳻ⲭᆹޘᙗᆈ⯁ˈ ڕ⴨৽ˈᡰᴹⲴᙕᙗਓᴽ∂ᙗ⹄ウ㔃᷌৽༽䇱ᇎˈCP4 EPSPS 㳻ⲭሩପңࣘ⢙Ⲵ ᓧ⋑ᴹԫօн㢟ᖡ૽ǄަѝˈᴰᯠᆼᡀⲴ⹄ウ׍ᦞ EPA OPPTS 870.1100 ઼ OECD Ự⍻ᤷই TG-425 䘋㹼Ǆབྷ㛐ᵶ㧼㺘䗮㓟ॆⲴ CP4 EPSPS 㳻ⲭ䙊䗷㓿ਓᯩᔿԕ 2000 mg/kg փ䟽ࡲ䟿অ⅑♼㛳䳼䳴਴ 10 ਚ CD-1 ሿ啐Ǆ⭡Ҿ൘ሿ啐ᆈ⍫⦷ǃѤ ᒺ㺘⧠ǃփ䟽ǃփ䟽໎࣐ǃ伏⢙⎸㙇ᡆབྷփ⯵⨶ᆖᯩ䶒൷ᵚਁ⧠о༴⨶⴨ޣⲴн 㢟৽ᓄˈഐ↔⺞ᇊ CP4 EPSPS 㳻ⲭⲴᰐ᰾ᱮᦏᇣ֌⭘ࡲ䟿˄NOAEL˅㠣ቁѪ䈅傼ᴰ儈ࡲ䟿ণ 2000 mg/kg փ䟽Ǆ 䪸ሩߌъ䜘Ҿ 2015 ᒤ 6 ᴸ઼ 9 ᴸє⅑ޣҾሿ啐ᙕᙗ∂ᙗ䈅傼ᮠᦞⲴ਼ṧ⯁ 䰞ˈᆏኡ䜭ޜਨ޽⅑䇔ⵏ⹄ウҶߌъ䜘 2031 ਧޜ੺ѝޣҾ㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼Ⲵ⴨ޣ㾱≲ǄᡁԜ⌘᜿ࡠˈỰ⍻ḷ߶ѝ㿴ᇊҶᴰབྷ㙀ਇࡲ䟿⌅˄MTD˅઼ॺ ᮠ㠤↫ࡲ䟿⌅˄LD50˅ǄสҾ䇱ᦞᵳ䟽⌅ˈབྷ䟿Ⲵᆹޘᙗ䇴ԧ䍴ᯉԕ৺㠚 1996 ᒤԕᶕᣇ㥹⭈㟖֌⢙൘ޘц⭼㤳ത޵Ⲵབྷ䶒〟⿽Ἵ൷ਟ䇱ᇎ CP4 EPSPS 㳻ⲭⲴ 伏⭘ᆹޘᙗǄഐ↔ˈLD50 ᯩ⌅ᒦн䘲⭘Ҿ䈨ྲ CP4 EPSPS 䘉ṧᰐᇣⲴ㳻ⲭǄᆏ ኡ䜭ޜਨ৲➗Ҷഭ䱵к䙊㹼Ⲵ OECD Ự⍻ᤷই TG 425 ઼ EPA OPPTS 870.1100 ѝ᧕䘁Ҿᴰབྷ㙀ਇࡲ䟿⌅Ⲵ䲀ᇊࡲ䟿⌅˄Limit Dose˅ᶕ䘋㹼ᙕᙗ∂ᙗ䈅傼Ǆ ؍OECD Ự⍻ᤷই TG 425 ᰾⺞ᤷࠪ˖ĀࠪҾሩࣘ⢙⾿࡙Ⲵ㘳㲁ˈ䲔䶎䈕䈅傼о ㌫ˈ੖ࡉн啃࣡֯⭘ GHS ⴞᖅ 5 㤳ത˄2000~5000ޣᣔӪ㊫ǃࣘ⢙৺⧟ຳᴹⴤ᧕ ਨ⋑ᴹ֯⭘ 2000 mg/kgޜmg/kg˅ᶕ䘋㹼ࣘ⢙䈅傼Ǆā㘳㲁ࡠԕлһᇎˈᆏኡ䜭 ԕкⲴ♼㛳ࡲ䟿䘋㹼ሿ啐Ⲵᙕᙗ㓿ਓ∂ᙗ⹄ウ˖ ᓧᒦᰐডᇣǄ㠚ڕ⢙ཊᒤⲴ୶ъॆᓄ⭘ᮠᦞ㺘᰾ CP4 EPSPS 㳻ⲭሩӪ઼ࣘ˅1 Ӿ 1996 ᒤᣇ㥹⭈㟖བྷ䉶୶ъॆ⿽Ἵԕᶕˈਜ਼ᴹ CP4 EPSPS 㳻ⲭⲴߌӗ૱ ˈ伏⭘Ǆ൘䗷৫ 20 ᒤޘ伏⢙׋ᓄ䬮ѝᆈ൘ᒦ㻛Ӫ㊫઼ࣘ⢙ᆹ⨳ޘቡаⴤ൘ ޘ⨳ᐢ㓿ᴹ 488 亩⅑ޣҾ CP4 EPSPS 㳻ⲭᆹޘᙗⲴᇑḕᡆ䇴ᇑˈᗇࠪⲴ㔃 ᓧ⋑ᴹн㢟ᖡ૽Ǆṩᦞഭ䱵ߌъڕ⢙䇪൷䇔Ѫ CP4 EPSPS 㳻ⲭሩӪ㊫઼ࣘ ⭏⢙ᢰᵟ㓴㓷˄ISAAA˅ᴰᯠⲴᙫ㔃ᣕ੺ˈࡠ 2014 ᒤˈ਴⿽অ㓟ᣇ䲔㥹ࡲ ᙗ⣦Ⲵ䖜สഐ֌⢙⿽Ἵ䶒〟儈䗮 1.03 ӯޜ产ˈਖᴹ 0.51 ӯޜ产ᣇ㲛ᣇ䲔㥹

XX ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ࡲ༽ਸᙗ⣦֌⢙ˈޡ䇑ঐޘ⨳䖜สഐ֌⢙⿽Ἵᙫ䶒〟Ⲵ㓖 85%ˈᒦᴹ䎵䗷 1000 ӯཤࣘ⢙㻛侢லਜ਼ᴹ䖜สഐ֌⢙˄བྷ䜘࠶ᱟᣇ㥹⭈㟖֌⢙˅Ⲵ侢ᯉǄ 2˅䲿ᵜ⭣䈧ҖᨀӔⲴབྷ䟿ᆹޘᙗ䇴ԧᮠᦞҏ䏣ԕ䇱ᇎ CP4 EPSPS 㳻ⲭⲴᆹޘ ᓧᴹн࡙ڕᙗ˖CP4 EPSPS оᐢ⸕Ⲵ∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃ䗷᭿৏ᡆሩ ᖡ૽Ⲵ⭏⢙⍫ᙗ㳻ⲭѻ䰤൷нާᴹ㔃ᶴ⴨լᙗ˗CP4 EPSPS оӪ㊫઼ࣘ⢙ ᰕᑨ伏⭘Ⲵᒦᐢ䇱᰾ᱟᆹޘⲴἽ⢙㳻ⲭ˄ྲἽ⢙޵Ⓚ EPSPS 㳻ⲭ˅ᴹᖸ儈 ਼Ⓚᙗˈ㘼䘉Ӌ㳻ⲭ൷ާᴹ䮯ᵏᆹޘ伏⭘শਢ˗CP4 EPSPS 㳻ⲭሩ✝нっ ᇊˈ䶎ᑨᇩ᱃൘⦹㊣✩ࡦ䗷〻ѝཡ⍫ˈᒦ൘⎸ॆ⏢ѝՊ㻛䗵䙏⎸ॆᦹˈн ާᴹ㌫㔏ᙗ᳤䵢䰞仈Ǆ 3˅ṩᦞѝഭӪᒣ൷⎸䍩⦹㊣Ⲵ䈳ḕᛵߥ˄↓᮷ㅜ 95 亥㺘 21˅ˈԕѝഭӪᒣ൷ փ䟽㓖 50 kg 䇑ˈ⇿ཙ⇿ޜᯔփ䟽Ⲵ⦹㊣⎸䍩䟿ࡉѪ 0.2 gǄ㔃ਸ MON 87427 ⦹㊣㊭㋂ѝ CP4 EPSPS 㳻ⲭⲴᒣ൷㺘䗮≤ᒣ˄3.6 μg/g 㓴㓷勌䟽˅ˈ⭡↔ՠ ㇇ѝഭӪ㗔䙊䗷㟣伏ᩴޕ MON 87427 ⦹㊣㊭㋂㘼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ нਟ㜭䗮ࡠޕ᳤䵢 MOE 儈䗮 2.76 × 106Ǆ䘉аᮠ٬⴨ᖃབྷˈ䙊ᑨⲴ㟣伏ᩴ 䈕ᮠ䟿㓗Ⲵ᳤䵢䟿Ǆ ഐ↔ˈਜ਼ᴹ CP4 EPSPS 㳻ⲭⲴߌӗ૱ᐢ㓿൘ޘ⨳㤳ത޵㻛ᆹޘᓄ⭘Ҷ䘁 20 ᙗ䇱ޘҾ CP4 EPSPS Ⲵᆹޣᒤˈᴤ儈♼㛳ࡲ䟿Ⲵᙕᙗ∂ᙗ⹄ウᒦн㜭ᨀ׋ᴤཊ ᦞǄ↔ཆˈ儈ࡲ䟿♼㛳ᖰᖰ൘ᢰᵟк䳮ԕᇎ⧠˖ഐ㳻ⲭ䍘ⓦ䀓ᓖᴹ䲀ˈ㾱䗮ࡠ儈 ࡲ䟿♼㛳ᗵ享ᨀ儈♼㛳փ〟ᡆᱟ໎࣐♼㛳⅑ᮠˈ㘼䘉є⿽ᯩᔿਟ㜭䜭Պᕅ䎧ᇎ傼 ࣘ⢙нᗵ㾱Ⲵᓄ◰঻࣋ˈᖸᴹਟ㜭䇙ᇎ傼㔃᷌␧⏶н␵Ǆ㔬кᡰ䘠ˈޘ䶒Ⲵ伏⭘ ⭘শਢ൷ᐢ䇱ᇎҶ CP4 EPSPS 㳻ⲭⲴ伏⭘઼侢⭘ᆹ֯ޘᙗ䇴ԧ઼䮯ᵏⲴᆹޘᆹ ޘᙗˈᯠ䘁ᆼᡀⲴሿ啐ᙕᙗ㓿ਓ∂ᙗ⹄ウ˄ᢰᵟᣕ੺ 14˅ҏ޽а⅑䇱ᇎҶ䖜ส ᓧᒦ⋑ᴹн㢟ᖡ૽Ǆڕ⢙ഐ⦹㊣ MON 87427 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭሩӪ㊫઼ࣘ

৲㘳᮷⥞˖ ᓧ⣦ߥᶑḕᣕ੺ѻҼ˄2002˅ˉ㟣伏о㩕ޫڕ㘏ࠔ㤡, ᶘᲃݹ. 2006. ѝഭት≁㩕ޫо .1 ㍐ᩴޕ⣦ߥ, pp. 21-33, Ӫ≁ছ⭏ࠪ⡸⽮. 2. ǉ䖜สഐἽ⢙ᆹޘ䇴ԧᤷইǊ˄ѝഭߌъ䜘ˈ2010˅ 3. Clive, J. 2014a. Global status of commercialized biotech/GM crops: 2014. ISAAA Brief No. 49. International Service for the Acquisition of Agri-Biotech Applications. Ithaca, NY. USA. 4. Codex Alimentarius (2009). Foods derived from modern biotechnology. Rome, Italy, Codex Alimentarius Commission, Joint FAO/WHO Food Standards Programme, Food and Agriculture Organization of the United Nations. Second Edition. 5. Kuiper, H. A., et al. (2013). "New EU legislation for risk assessment of GM food: no scientific justification for mandatory animal feeding trials." Plant Biotechnology Journal 11(7): 781-784.

XXI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߌъ䜘ޣҾMON 87427ᆹޘ䇴ԧ⭣䈧Ⲵഎ༽࠭ ˄2015ᒤ6ᴸ24ᰕ˅

XXII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ҿߌъ䜘ᢩ༽ؑ࠭Ⲵ䈤᰾ޣਨޜᆏኡ䜭

䰞仈˖ᙕᙗ∂ᙗ䈅傼ᵚ᤹➗ḷ߶ǉ㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼Ǌ˄ߌъ䜘 2031 ਧޜ ੺-16-2013˅Ⲵ㾱≲ᆼᡀǄ

䈤᰾˖㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣ MON 87427 ѝᕅޕҶⓀ㠚Ҿ൏༔ߌᵶ㧼 CP4 㧼 ṚⲴ cp4 epsps 㕆⸱ᒿࡇˈަ㺘䗮ӗ⢙ 5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦 ˄CP4 EPSPS˅ሩ㥹⭈㟖ⲴӢ઼ᙗ䶎ᑨվˈഐ↔㜭ཏ㙀ਇ㥹⭈㟖㊫䲔㥹ࡲǄᆏኡ 䜭ޜਨ൘ᔰਁ MON 87427 㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣Ⲵ䗷〻ѝˈ䘋㹼Ҷབྷ䟿Ⲵᆹ ޘᙗ䇴ԧˈ䘉Ӌ䍴ᯉҾ 2012 ᒤ 7 ᴸ俆⅑ᨀӔ㔉ѝഭߌъ䜘ᇑṨǄሩҾᵜ⅑᭦ࡠ ਨ䲔ሩ৏ᴹᢰᵟ䍴ᯉ䘋а↕▴␵ཆˈ䘈㺕ݵᨀޜҾᙕᙗ∂ᙗ䈅傼Ⲵ䰞仈ˈᡁޣⲴ ӔҶᯠⲴ䈅傼ᮠᦞ˖࡙⭘བྷ㛐ᵶ㧼㌫㔏㺘䗮㓟ॆⲴ CP4 EPSPS 㳻ⲭሩሿ啐䘋㹼 Ⲵᙕᙗ㓿ਓ∂ᙗ⹄ウǄ䈕⹄ウᣕ੺ޘ᮷䈧㿱ᵜ⭣䈧Җл޼ᢰᵟᣕ੺ 14Ǆ 䈕亩ᯠⲴ CP4 EPSPS ᙕᙗ∂ᙗ⹄ウ⭡ Smedley ㅹӪҾ 2015 ᒤ 6 ᴸᆼᡀˈ䈅 ┑㾱≲а㠤ˈҏޣ傼䇮䇑ѫ㾱׍ᦞ EPA OPPTS 870.1100ˈо OECD TG 425 ѝ⴨ 䏣ߌъ䜘亱ਁⲴ⴨ޣḷ߶ NY-2031-16-2013Ǆߌъ䜘ޣҾ㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼 Ⲵḷ߶ѝ᧿䘠Ҷє⿽ᯩ⌅˖LD50 ઼ MTDǄަѝˈLD50 ⌅ᒦн䘲⭘Ҿ䖜สഐ֌⢙ ѝᯠ㺘䗮㳻ⲭⲴᆹޘᙗ䇴ԧǄ֌Ѫޣ䭞Ⲵ㩕ޫ⢙䍘ˈ㔍བྷ䜘࠶㳻ⲭ䍘䜭ᱟᰐ∂ⲴǄ བྷ䜘࠶ᩴޕⲴ㳻ⲭ䍘䜭Պ㓿䗷≤䀓઼㳻ⲭ䞦Ⲵ䱽䀓䗷〻ˈਈᡀঅњ≘ส䞨ᡆ⸝䬮㛭⇥ˈᴰ㓸൘փ޵⭘Ҿ㳻ⲭ䍘ⲴਸᡀᡆަԆԓ䉒䙄ᖴ˄Delaney, Astwood et al. 2008˅Ǆ伏⢙ѝⲴ㔍བྷཊᮠ㳻ⲭ䍘䜭䘋㹼ҶᆹޘᙗỰ⍻ˈ൷ᵚਁ⧠ާᴹ∂ᙗ ˄Hammond and Cockburn 2008˅Ǆഐ↔ˈӪ㊫㟣伏ѝࣘ⢙઼Ἵ⢙ᶕⓀⲴ㳻ⲭ䍘 ൷ާᴹ䮯ᵏᆹޘ伏⭘শਢǄᒦфˈ㠚 1996 ᒤ䎧ᐢሩ䎵䗷 25 ⿽н਼Ⲵ䖜สഐ㳻ⲭ 䘋㹼Ҷᙕᙗ∂ᙗ⹄ウˈᵚਁ⧠о⍻䈅⢙⴨ޣⲴሿ啐䶎↓ᑨ↫ӑ˄CERA, 2015˅ ٬Ǆഐ↔ˈMTD ᯩ⌅ᱟ⭘Ҿ䇴ՠ䖜㘼ᵚ㿲ሏࡠࣘ⢙↫ӑণ᜿ણ⵰ᰐ⌅⍻ᇊ LD50 สഐ֌⢙ѝ㳻ⲭ䍘ԫօ▌൘ডᇣⲴᴤѪ、ᆖⲴᯩ⌅Ǆ NY-2031-16-2013 ѝޣҾ MTD Ⲵᯩ⌅ᒦ⋑ᴹ㿴ᇊᴰվਟ᧕ਇࡲ䟿≤ᒣˈ䘉 оഭ޵ཆⲴ䈅傼ḷ߶઼ᤷইᱟа㠤ⲴǄ൘ᯠⲴᙕᙗ∂ᙗ⹄ウⲴࡲ䟿䇮ᇊᰦˈᡁԜ ৲㘳Ҷ EPA OPPTS 870.1100 ઼ OECD TG 425 ѝⲴ䲀ᇊࡲ䟿⌅˄limit dose˅Ǆ EPA OPPTS 870.1100 ઼ OECD TG 425 ൷Ѫ䙊⭘Ⲵᙕᙗ∂ᙗ䈅傼ᤷইˈ㘼нᱟ⢩ ࡛䪸ሩ㳻ⲭᙕᙗ∂ᙗ⹄ウࡦᇊⲴǄOECD TG 425 ѝ⢩࡛ᤷࠪˈࠪҾࣘ⢙⾿࡙Ⲵ ᓧᴹⴤ᧕ᖡ૽ˈнڕ⢙㘳㲁ˈྲ᷌ᴹ䍴ᯉ㜭ཏ㺘᰾⍻䈅⢙䍘нཚਟ㜭ሩӪ㊫ᡆࣘ 啃࣡䘋㹼䎵䗷 2000 mg/kg փ䟽Ⲵ䲀ᇊࡲ䟿⌅⹄ウǄ䲔Ҷࣘ⢙⾿࡙ഐ㍐ཆˈаӋ о㳻ⲭⓦ䀓ᓖ઼♼㛳ࡦࡲࡦ༷⴨ޣⲴᢰᵟᙗഠ䳮ҏਟ㜭䲀ࡦᙕᙗ∂ᙗ䈅傼ѝ㳻 ⲭⲴᴰ儈♼㛳ࡲ䟿˄ণׯᱟ䗮ࡠ 2000 mg/kg bw ҏᖸഠ䳮˅Ǆ儈ࡲ䟿♼㛳䴰㾱ࡦ ࡠǄ䘉⿽ᛵߥлᓄᖃቭᴰབྷਟ㜭ڊ儈ᓖ⎃㕙ⲴࡦࡲˈሩᴹӋ㳻ⲭ㘼䀰ਟ㜭ᖸ䳮༷ MOE˄Bushey, Bannon et al. 2014˅Ǆ↔ཆˈ䘈ᴹަԆаӋо֯ 䲀٬ޘൠᨀ儈ᆹ ⭘儈ᓖ⎃㕙㳻ⲭ⴨ޣⲴᢰᵟ䳮仈Ǆ∄ྲḀӋ㳻ⲭ൘♼㛳ⓦࡲѝⲴⓦ䀓ᓖᴹ䲀ˈᰐ ⌅ᙫᱟ䗮ࡠ g/kg ᮠ䟿㓗Ⲵࡲ䟿≤ᒣㅹㅹǄ⭡Ҿབྷ䟿Ⲵᆹޘᙗ䇴ԧ䍴ᯉ൷㺘᰾ˈ ᓧ⋑ᴹн㢟ᖡ૽ˈഐ↔ˈᡁԜ䘹ᤙҶ 2000 mg/kg փڕ⢙CP4 EPSPS ሩӪ㊫઼ࣘ 䟽Ⲵ♼㛳ࡲ䟿޽⅑ሩ CP4 EPSPS 䘋㹼ᙕᙗ∂ᙗ⹄ウǄᒦфˈ䈕ࡲ䟿⴨ᖃҾᡀᒤ

XXIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ӫ⇿ᰕ伏⭘㓖 27.6 ੘Ⲵ MON 87427 ⦹㊣㊭㋂ˈ䘉аࡲ䟿ԓ㺘Ҷ㟣伏ᩴޕнਟ㜭䗮ࡠⲴ≤ᒣǄ Smedley˄2015˅⹄ウᱟ൘ᔪ・Ҷབྷ㛐ᵶ㧼㺘䗮㌫㔏ӗ⭏㳻ⲭо MON 87427 ⦹㊣ѝᯠ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭѻ䰤Ⲵㅹ਼ᙗส⹰к˄ᢰᵟᣕ੺ 5˅ˈ࡙⭘བྷ㛐 ᵶ㧼㺘䗮㓟ॆⲴ CP4 EPSPS 㳻ⲭԕ㓿ਓᯩᔿԕ 2000 mg/kg փ䟽ࡲ䟿অ⅑♼㛳䳼䳴਴ 10 ਚ CD-1 ሿ啐Ǆ਼ᰦ䘋㹼䈅傼Ⲵ䘈ᴹⓦࡲሩ➗㓴ԕ৺ԕ਼ㅹࡲ䟿ሿ⢋㹰 ␵㳻ⲭ˄BSA˅♼㛳Ⲵ㳻ⲭሩ➗㓴Ǆᡰᴹࣘ⢙൘♼㛳ࡽ൷⾱伏༴⨶ˈ♼㛳ਾ⇿ཙ є⅑㿲ሏሿ啐ᱟ੖↫ӑᡆᱟ㺘⧠ࠪѝ∂⯷⣦ˈ㿲ሏᵏѪ 14 ཙǄㅜ 14 ཙᰦሶᡰᴹ ࣘ⢙༴↫ˈ䘋㹼བྷփ䀓ࢆǄ⹄ウ㔃᷌㺘᰾ˈᰐ䇪Ӿሿ啐ᆈ⍫⦷ǃѤᒺ㺘⧠ǃփ䟽ǃ փ䟽໎࣐ǃ伏⢙⎸㙇ᡆབྷփ⯵⨶ᆖᯩ䶒ⴻˈ൷ᵚਁ⧠о༴⨶⴨ޣⲴн㢟৽ᓄǄഐ ↔ˈ䙊䗷䈕ሿ啐ᙕᙗ∂ᙗ⹄ウᗇࡠⲴ CP4 EPSPS 㳻ⲭⲴᰐ᰾ᱮᦏᇣ֌⭘ࡲ䟿 ˄NOAEL˅㠣ቁѪ⹄ウᴰ儈ࡲ䟿 2000 mg/kg փ䟽ˈণ CP4 EPSPS 㳻ⲭⲴ㓿ਓᙕ ᙗ∂ᙗᴰབྷ㙀ਇࡲ䟿Ѫ 2000 mg/kg փ䟽Ǆṩᦞ㘏ࠔ㤡઼ᶘᲃݹ˄2006˅൘ǉѝ ߥǊࡇࠪ⣦ޕᓧ⣦ߥᶑḕᣕ੺ѻҼ˄2002˅üü㟣伏о㩕ޫ㍐ᩴڕഭት≁㩕ޫо ⲴѝഭӪᒣ൷⎸䍩⦹㊣Ⲵ䈳ḕᛵߥˈѝഭӪ⇿ཙ⇿Ӫᒣ൷Ⲵ⦹㊣⎸䍩䟿Ѫ 9.9 gˈ ṩᦞѝഭӪᒣ൷փ䟽㓖 50 kgˈᣈ㇇ᡀ⇿ཙ⇿ޜᯔփ䟽⎸䍩䟿ࡉѪ 0.2 gǄ㔃ਸ MON 87427 ⦹㊣㊭㋂ѝ CP4 EPSPS 㳻ⲭⲴᒣ൷㺘䗮≤ᒣ˄3.6 μg/g 㓴㓷勌䟽˅ˈ ⭡↔ՠ㇇ѝഭӪ㗔䙊䗷㟣伏ᩴޕ MON 87427 ⦹㊣㊭㋂㘼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ ᳤䵢 MOE 儈䗮 2.76 × 106Ǆഐ↔ˈᯠ䘁ᆼᡀⲴሿ啐ᙕᙗ㓿ਓ♼㛳⹄ウ޽⅑䇱ᇎˈ CP4 EPSPS 㳻ⲭሩ䈅傼ሿ啐⋑ᴹн㢟ᖡ૽ˈ䈕ᆹޘ♼㛳ࡲ䟿⴨ᖃҾањᲞ䙊ᡀᒤ Ӫ⇿ཙ伏⭘㓖 27.6 ੘Ⲵ MON 87427 ⦹㊣㊭㋂᡽㜭䗮ࡠⲴ CP4 EPSPS 㳻ⲭ᳤䵢 ≤ᒣǄ ሩҾ䖜สഐ֌⢙Ⲵᆹޘᙗ䇴ԧˈवᤜѝഭ൘޵Ⲵޘ⨳਴ⴁ㇑ᵪᶴⲴ⌅㿴ᤷই ѝ൷ᤷࠪˈ׍ᦞњṸ࠶᷀৏ࡉᨀ׋ᙕᙗ∂⨶ᆖ䍴ᯉˈᖃ䇱ᦞᵳ䟽࠶᷀ᯩ⌅㜭ཏ㺘 ⭘শਢᰦˈᙕᙗ∂⨶ᆖ䍴ᯉᒦ䶎ᗵ䴰Ǆྲ֯ޘ᰾䖜สഐ⭏⢙ѝᯠ㺘䗮㳻ⲭާᴹᆹ ߌъ䜘亱ᐳⲴǉ䖜สഐἽ⢙ᆹޘ䇴ԧᤷইǊ˄2010˅ѝ㿴ᇊĀᖃᯠ㺘䗮㳻ⲭ䍘ᰐ ᙗ䍴ᯉн䏣ᰦˈᗵ享ᨀ׋ᙕᙗ㓿ਓ∂ᙗ䍴ᯉāǄ਼ṧˈ䍏䍓ޘ伏⭘শਢˈᆹޘᆹ ᢗ㹼㋞ߌ㓴㓷/ц⭼ছ⭏㓴㓷㚄ਸ伏૱ḷ߶䇑ࡂⲴ伏૱⌅ިငઈՊ Codex ҏਁᐳ Ҷഭ䱵Პ䙽䟷⭘Ⲵᤷই˄Codex Alimentarius 2009˅ˈ᧘㦀֯⭘䇱ᦞᵳ䟽⌅ሩ䖜 สഐἽ⢙ᶕⓀ伏૱䘋㹼ᆹޘᙗ䇴ՠǄ2009 ᒤ⡸Ⲵ Codex ޡ䇶᮷Ԧѝᤷࠪ˖Āྲ ᷌伏⢙ѝᆈ൘Ⲵ㳻ⲭоᐢ⸕ਟᆹޘ伏⭘㳻ⲭѻ䰤ᒦᰐ⴨լᙗˈਟ㜭䴰㾱䘋㹼䘲ᖃ Ⲵ㓿ਓ∂⨶ᆖ⹄ウāǄ֌Ѫޣ䭞Ⲵ㩕ޫ⢙䍘ˈ㔍བྷ䜘࠶㳻ⲭ䍘䜭ᱟᰐ∂ⲴǄਚᴹ ḀӋ⭏⢙փӗ⭏Ⲵᶱቁᮠ㳻ⲭ䍘ᱟᐢ⸕ᴹ∂ⲴǄབྷ䜘࠶ᴹ∂㳻ⲭ䜭ᱟ䙊䗷ᙕᙗ֌ ⭘ᵪࡦӗ⭏∂ᙗ˄Sjoblad, McClintock et al. 1992, Hammond and Fuchs 1998, Pariza and Johnson 2001˅Ǆҏᴹᶱቁᮠ㳻ⲭֻཆˈѫ㾱ᱟḀӋᣇ㩕ޫ㳻ⲭˈྲἽ⢙ࠍ ↺⢩䳶㍐Ǆн䗷ˈ䙊䗷⭏⢙ؑ᚟ᆖᯩ⌅ਟԕ⺞䇔䖜สഐ֌⢙ѝᯠ㺘䗮㳻ⲭо䘉Ӌ 㳻ⲭᱟ੖ާᴹ㔃ᶴ⴨լᙗ˄Hammond, et al. 2013˅Ǆ Smedley˄2015˅⹄ウᒦ䶎⭡Ҿሩ CP4 EPSPS 㳻ⲭⲴᆹޘᙗᆈ⯁ˈ㘼ᱟࠪҾ 䟽㿶ߌъ䜘уᇦ൘৽侸᜿㿱ѝᨀࠪⲴሩнㅖਸ䈅傼ḷ߶Ⲵᙕᙗ⹄ウ∂ᙗ⹄ウᯩ ⌅Ⲵ⯁㲁Ǆо亴ᵏ㔃᷌а㠤ˈSmedley˄2015˅Ⲵ⹄ウ㔃᷌䘋а↕䇱ᇎҶѻࡽᐢ ᙗ㔃䇪ǄޘҾ CP4 EPSPS 㳻ⲭⲴᆹޣ㓿ᨀ׋㔉ߌъ䜘Ⲵєԭᙕᙗ∂ᙗ⹄ウᣕ੺ѝ XXIV ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ѻࡽሩ CP4 EPSPS 㳻ⲭ䘋㹼Ⲵᙕᙗ∂ᙗ⹄ウ˄࠶࡛Ѫ 1993 ᒤ Hammond Ⲵ ⹄ウ઼ 2002 ᒤ Bonnette Ⲵ⹄ウ˅൷׍ᦞ EPA OPPTS 870.1100 ᤷই઼ഭ䱵䙊⭘ Ⲵ OECD Test Guideline 425 ᤷই䘋㹼ǄHammond Ҿ 1993 ᒤᆼᡀⲴ⹄ウѝˈCP4 EPSPS 㳻ⲭԕঅаࡲ䟿ਓᴽ♼㛳а㓴䳼䳴਴ 10 ਚ CD-1 ሿ啐ˈ਼ᰦ䇮а㓴⴨լ 㳻ⲭࡲ䟿≤ᒣⲴሩ➗ሿ㓴ˈԕሿ⢋㹰␵㳻ⲭ˄BSA˅ਓᴽ♼㛳ሿ啐˗♼㛳ࡽ䇙ሿ 啐⾱伏オ㞩ˈ⇿ཙє⅑㿲ሏᱟ੖ᴹሿ啐↫ӑᡆᱟѝ∂⯷⣦ˈ㿲ሏᵏ࠶࡛Ѫ㔉㦟ਾ 7 ཙ˄Hammond, 1993˅઼ 14 ཙ˄Bonnette, 2002˅˗⍫փ㿲ሏ㔃ᶏਾˈሶሿ啐༴ ↫ᒦ䘋㹼བྷփ䀓ࢆǄHammond˄1993˅ᙕᙗ∂ᙗ⹄ウѝ䘹ᤙ 572 mg/kg bw Ⲵࡲ ᆸՠ㇇Ⲵ⇿ᰕ᳤䵢䟿ᶑԦлӽ❦ާᴹ䏣ཏབྷⲴ᳤䵢؍൘儈ᓖ؍⺞䟿≤ᒣᱟѪҶ ᆸՠ㇇ѝᒦ⋑ᴹवᤜ伏⢙൘࣐ᐕǃ✩ࡦԕ৺⎸ॆ䗷〻؍㘼൘⇿ᰕ᳤䵢䟿Ⲵˈ䲀٬ ѝਁ⭏䱽䀓ሬ㠤ᴰਾӪփᖸ䳮ሩᆼᮤ㳻ⲭާᴹᴹ᜿ѹⲴ᳤䵢Ǆਖа亩ޣҾ CP4 EPSPS 㳻ⲭⲴᙕᙗ⹄ウᱟ NK063 ѝ㺘䗮Ⲵ CP4 EPSPS L214P 㳻ⲭˈަ⹄ウᣕ੺ ᙗᨀ׋ᴤཊޘҏҾ 2015 ᒤ 3 ᴸᨀӔ㔉ѝഭߌъ䜘ˈ⭘ҾѪ CP4 EPSPS 㳻ⲭⲴᆹ Ⲵ䇱ᦞ˄Bonnette, 2002˅Ǆ䘉а⹄ウਟⴤ᧕⭘Ҿր䇱 MON 87427 ⦹㊣ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭˈഐѪє㘵ӵᴹањ≘ส䞨Ⲵᐞᔲˈ㕆⸱ᒿࡇ⴨լᙗǄ ൘䖳ᯠⲴ⹄ウ˄Bonnette, 2002˅ѝˈ㿲ሏᵏѪ 14 ཙˈ♼㛳ࡲ䟿ҏᴤ儈˄817 mg/kg bw˅Ǆ䘉Ӌ⹄ウᒦн䘍㛼ѝഭ⴨ޣᤷই NY-2031-16-2013 ѝሩ䖜สഐ⭏⢙ᯠ㺘䗮㳻ⲭᙕᙗ∂ᙗ࠶᷀Ⲵ䈅傼䇮䇑㾱≲˖䘉Ӌ⹄ウ⺞ᇊᴰ儈♼㛳ࡲ䟿Ѫᴰབྷ㙀ਇࡲ 䟿˄MTD˅ˈᒦሩѻࡽᐢ⭡䇱ᦞᵳ䟽࠶᷀⌅⺞ᇊⲴ MON 87427 ѝ㺘䗮 CP4 EPSPS ᙗᨀ׋Ҷ䘋а↕䇱ᦞǄޘ㳻ⲭⲴᆹ བྷ䟿Ⲵᆹޘᙗ䇴ԧ䍴ᯉԕ৺㠚 1996 ᒤԕᶕᣇ㥹⭈㟖֌⢙൘ޘц⭼㤳ത޵Ⲵ བྷ䶒〟⿽Ἵ൷ਟ䇱ᇎ CP4 EPSPS 㳻ⲭⲴ伏⭘ᆹޘᙗǄᵜ⭣䈧ҖѝสҾ䇱ᦞᵳ䟽 ⌅ሩ MON 87427 ⦹㊣䘋㹼Ⲵ伏⭘ᆹޘᙗ䇴ԧ䍴ᯉवᤜ˖ ⭏⢙ؑ᚟ᆖ࠶᷀㺘᰾ˈCP4 EPSPS оᐢ⸕∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃ䗷᭿˅1 ˗˅ᓧᴹн࡙ᖡ૽Ⲵ⭏⍫⍫ᙗ㳻ⲭѻ䰤нާᴹ㔃ᶴ⴨լᙗ˄ᢰᵟᣕ੺ 6ڕ৏ᡆሩ 2˅CP4 EPSPS оӪ㊫઼ࣘ⢙ᰕᑨ伏⭘Ⲵᒦᐢ䇱᰾ᱟᆹޘⲴἽ⢙㳻ⲭ˄ྲἽ ⢙޵Ⓚ EPSPS 㳻ⲭ˅ᴹᖸ儈਼Ⓚᙗˈ㘼䘉Ӌ㳻ⲭ൷ާᴹ䮯ᵏᆹޘ伏⭘শਢ˗ 3˅փཆ⎸ॆ䈅傼㺘᰾ˈCP4 EPSPS 㳻ⲭ൘⁑ᤏ㛳⏢˄SGF˅ѝՊ㻛䗵䙏⎸ ॆᦹˈнާᴹ㌫㔏ᙗ᳤䵢䰞仈˄ᢰᵟᣕ੺ 8˅Ǆ 4˅CP4 EPSPS 㳻ⲭሩ✝нっᇊˈ㘼ᰕᑨ㟣伏ѝ⦹㊣䙊ᑨ䴰㾱✩ࡦˈ߿ቁҶ ާᴹᆼᮤ࣏㜭 CP4 EPSPS 㳻ⲭ൘㟣伏ѝⲴ᳤䵢ࠐ⦷˄Hammond and Jez, 2011˅Ǆ䈅傼㔃᷌㺘᰾ˈCP4 EPSPS 㳻ⲭ൘⑙ᓖ䎵䗷 75°C ᰦ࣏㜭⍫ᙗᆼޘ їཡ˄ᢰᵟᣕ੺ 8˅Ǆ 5˅CP4 EPSPS 㳻ⲭ൘ MON 87427 ⦹㊣㊭㋂ѝⲴ㺘䗮䟿ᖸվ˄3.6 μg/g 㓴㓷勌䟽ˈ㓖ঐ⦹㊣ᙫ㳻ⲭⲴ 0.004%˅ˈഐ↔䲀ࡦҶ䙊䗷ᰕᑨ⎸䍩 MON 87427 ⦹㊣ሩ CP4 EPSPS 㳻ⲭⲴ᳤䵢≤ᒣǄṩᦞ Hammond˄1993˅ሿ啐ᙕᙗ∂ᙗ ⹄ウ㔃᷌ NOAEL 㠣ቁѪ 572 mg/kg bwˈՠ㇇ MON 87427 ⦹㊣ѝ CP4 EPSPS 㳻ⲭሩѝഭӪ㗔㘼䀰ަ MOE Ѫ㠣ቁ 1.4 × 104 㠣 7.9 × 105ˈ˄㿱ᵜ⭣䈧Җㅜ 95 亥Ӫ㗔㟣伏ᩴޕ仾䲙䇴ՠ˅ˈᡰԕᴹ⨶⭡ᶕ᧘ᯝ伏⢙઼侢ᯉѝⲴ

XXV ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 CP4 EPSPS нՊሩӪ㊫઼ࣘ⢙䙐ᡀডᇣǄቡ MON 87427 ⦹㊣ѝⲴ CP4 EPSPS 㳻ⲭ㘼䀰ˈањփ䟽Ѫ 50 kg ⲴӪ䴰㾱⇿ཙ⎸䍩㓖 7900 kg ˄䎵 ˅䗷㠚䓛փ䟽 150 ؽ˅Ⲵ MON 87427 ⦹㊣㊭㋂᡽㜭䗮ࡠо Hammond˄1993 ሿ啐ᙕᙗ∂ᙗ⹄ウᡰ⭘ࡲ䟿བྷ㠤⴨ᖃⲴ CP4 EPSPS 㳻ⲭ᳤䵢≤ᒣǄ㘼 2015 ٬ᨀ儈ࡠ 2000 mg/kg ᒤᯠᆼᡀⲴ CP4 EPSPS ሿ啐ᙕᙗ∂ᙗ⹄ウሶ NOAEL bwˈᦞ↔䇑㇇ࠪⲴѝഭӪ㗔䙊䗷㟣伏ᩴޕ MON 87427 ⦹㊣㊭㋂㘼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ᳤䵢 MOE 儈䗮 2.76 × 106Ǆ ↔ཆˈᦞ ISAAA 㔏䇑ˈᡚ→ 2014 ᒤˈޘ⨳ᴹ䎵䗷 170 ⿽䖜สഐ֌⢙ӗ૱㧧 ᗇҶ⿽Ἵᢩ߶ˈ䘉Ӌӗ૱ѝѫ㾱वᤜ CP4 EPSPSǃPAT ઼ Bt 㳻ⲭㅹ㻛ӪԜ⟏⸕ Ⲵ㳻ⲭ˄Clive, 2014b; CERA, 2015˅Ǆᡰᴹ䖜สഐ֌⢙൘୶ъॆᓄ⭘ࡽ൷׍ᦞ䇱 ᦞᵳ䟽Ⲵᯩ⌅䘋㹼Ҷޘ䶒Ⲵᆹޘᙗ䇴ԧˈᒦ㓿䗷Ҷཊഭ⌅㿴ᵪᶴѕṬⲴᢰᵟᇑḕǄ 2014 ᒤޘ⨳䖜สഐ֌⢙˄ѫ㾱ᱟ㙀䲔㥹ࡲ઼ᣇ㲛֌⢙˅Ⲵ⿽Ἵ䶒〟ᐢ䗮 1.8 ӯޜ 产ˈঐޘ⨳ᙫ᫝⿽䶒〟Ⲵ 49%ˈ1996-2014 ᒤ䰤䖜สഐ֌⢙Ⲵ㍟〟⿽Ἵ䶒〟ᴤ䗮产ѻཊ˄Clive, 2014a˅Ǆ㘼֌Ѫㅜаֻ୶ъॆⲴᙗ⣦ˈCP4 EPSPS 㳻ⲭޜӯ 18 ާᴹ൘ޘ⨳伏⢙䬮ѝ䮯ᵏᆹޘ⎸䍩ⲴশਢǄ㠚Ӿ 1996 ᒤᣇ㥹⭈㟖བྷ䉶୶ъॆ⿽ 伏⢙׋ᓄ䬮ѝᆈ൘ᒦ㻛Ӫ⨳ޘἽԕᶕˈਜ਼ᴹ CP4 EPSPS 㳻ⲭⲴߌӗ૱ቡаⴤ൘ ㊫઼ࣘ⢙ᆹޘ伏⭘Ǆਜ਼ᴹ CP4 EPSPS Ⲵᣇ㥹⭈㟖֌⢙⇿ᒤ䜭ᴹ 30 ཊњӗ૱൘ 13 њഭᇦ⿽Ἵ䶒〟䎵䗷 7400 зޜ产˄Dill et al., 2008˅Ǆ㠚 1996 ᒤᣇ㥹⭈㟖֌⢙ ୶ъॆ⿽Ἵԕᶕˈᐢᴹ䎵䗷 1000 ӯཤࣘ⢙㻛侢லਜ਼ᴹ䖜สഐ֌⢙˄བྷ䜘࠶ᱟᣇ ᓧ઼⭏ӗ⦷Ⲵᣕ੺Ǆаㇷ㓿䗷਼㹼䇴䇞Ⲵڕ⢙㥹⭈㟖֌⢙˅Ⲵ侢ᯉˈ⋑ᴹᖡ૽ࣘ ᓧᖡ૽Ⲵ᮷ڕҾ侢லਜ਼ᴹ䖜สഐ֌⢙˄वᤜᣇ㥹⭈㟖֌⢙˅侢ᯉሩࣘ⢙⭏䮯઼ޣ 㔬䘠˄Eenennaam & Young, 2014˅ҏᤷࠪˈⴞࡽ䘈⋑ᴹԫօֻᆀ㺘᰾ਜ਼ᴹ䖜⥞ ᐢ㓿ᴹ 488⨳ޘˈᓧӗ⭏н࡙ᖡ૽Ǆ൘䗷৫ 20 ᒤڕสഐ֌⢙侢ᯉՊሩࣘ⢙⭏䮯઼ 亩⅑ޣҾ CP4 EPSPS 㳻ⲭᆹޘᙗⲴᇑḕᡆ䇴ᇑˈᗇࠪⲴ㔃䇪൷䇔Ѫ CP4 EPSPS ᓧ⋑ᴹн㢟ᖡ૽Ǆڕ⢙㳻ⲭሩӪ㊫઼ࣘ ⭘শਢԕ৺ཊ⅑䘋㹼Ⲵሿ֯ޘᙗ䇴ԧǃ䮯ᵏⲴᆹޘ䶒Ⲵ伏⭘ᆹޘˈ㔬кᡰ䘠啐ਓᴽ∂⨶ᆖ⹄ウ㔃᷌൷䇱ᇎҶ CP4 EPSPS 㳻ⲭⲴ伏⭘઼侢⭘ᆹޘᙗǄ

৲㘳᮷⥞˖ ᓧ⣦ߥᶑḕᣕ੺ѻҼ˄2002˅ˉ㟣伏оڕ㘏ࠔ㤡, ᶘᲃݹ. 2006. ѝഭት≁㩕ޫо .1 㩕ޫ㍐ᩴޕ⣦ߥ, pp. 21-33, Ӫ≁ছ⭏ࠪ⡸⽮. 2. ǉ䖜สഐἽ⢙ᆹޘ䇴ԧᤷইǊ˄ѝഭߌъ䜘ˈ2010˅ 3. Bushey, D. F., et al. (2014). "Characteristics and safety assessment of intractable proteins in genetically modified crops." Regulatory Toxicology and Pharmacology 69(2): 154-170. 4. Center for Environmental Risk Assessment (CERA). 2015. GMO crop database. [accessed Jun 10, 2015] 5. Clive, J. 2014a. Global status of commercialized biotech/GM crops: 2014. ISAAA Brief No. 49. International Service for the Acquisition of Agri-Biotech Applications. Ithaca, NY. USA. 6. Clive, J. 2014b. Executive summary. Global status of commercialized biotech/GM crops: 2014. ISAAA Brief No. 49. International Service for the Acquisition of

XXVI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Agri-Biotech Applications. Ithaca, NY. USA. 7. http://www.isaaa.org/resources/publications/briefs/49/executivesummary/default.asp 8. Codex Alimentarius (2009). Foods derived from modern biotechnology. Rome, Italy, Codex Alimentarius Commission, Joint FAO/WHO Food Standards Programme, Food and Agriculture Organization of the United Nations. Second Edition. 9. Delaney, B., et al. (2008). "Evaluation of protein safety in the context of agricultural biotechnology." Food and Chemical Toxicology 46(Supplement 2): S71-S97. 10. Dill, G. M., et al. (2008). "Glyphosate-resistant crops: Adoption, use and future considerations." Pest Management Science 64: 326-331. 11. Eenennaam, A. L. v. and A. E. Young (2014). "Prevalence and impacts of genetically engineered feedstuffs on livestock populations." Journal of Animal Science 92(10): 4255-4278. 12. EFSA (2009). "Scientific opinion of the scientific committee: "Existing approaches incorporating replacement, reduction and refinement of animal testing: Applicability in food and feed risk assessment"." EFSA Journal 1052: 1-77. 13. Faustman, E. M. and G. S. Omenn (2008). Risk assessment. Casarett and Doull's Toxicology - The Basic Science of Poisons. C. D. Klassen. New York, New York, McGraw-Hill: 107-128. 14. Hammond, B. G. and A. Cockburn (2008). The safety assessment of proteins introduced into crops developed through agricultural biotechnology: A consolidated approach to meet current and future needs. Food Safety of Proteins in Agricultural Biotechnology. Boca Raton, Florida, CRC Press, Inc.: 259-288. 15. Hammond, B. and R. L. Fuchs (1998). "Safety evaluation of new varieties of food crops developed through biotechnology." Biotechnology and Safety Assessment.: 61-79. 16. Hammond, B. G. and J. M. Jez (2011). "Impact of food processing on the safety assessment for proteins introduced into biotechnology-derived soybean and corn crops." Food and Chemical Toxicology 49(4): 711-721. 17. Hammond, B., et al. (2013). "Toxicological evaluation of proteins introduced into food crops." Crit Rev Toxicol 43: 25-42. 18. ISAAA. 2014. Global biotech/GM crop plantings increase 100-fold from 1996. International Service for the Acquisition of Agri-Biotech Applications. Manila, Philippines. 19. Kuiper, H. A., et al. (2013). "New EU legislation for risk assessment of GM food: no scientific justification for mandatory animal feeding trials." Plant Biotechnology Journal 11(7): 781-784. 20. Liener, I. E. (1994). "Implications of antinutritional components in soybean foods." Critical Reviews in Food Science and Nutrition 34: 31-67. 21. Pariza, M. W. and E. A. Johnson (2001). "Evaluating the safety of microbial enzyme preparations used in food processing: update for a new century." Regul Toxicol Pharmacol 33(2): 173-186. 22. Sjoblad, R. D., et al. (1992). "Toxicological considerations for protein components of biological pesticide products." Regul. Toxicol. Pharmacol. 15(1): 3-9.

XXVII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߌъ䜘ޣҾMON 87427ᆹޘ䇴ԧ⭣䈧Ⲵഎ༽࠭ ˄2014ᒤ12ᴸ11ᰕ˅

XXVIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ҿߌъ䜘ᢩ༽ؑ࠭Ⲵ䈤᰾ޣਨޜᆏኡ䜭

䰞仈 1˖ ᙕᙗ∂ᙗ䈅傼ᵚ᤹ߌъ䜘 2031 ਧޜ੺-16-2013Ā䖜สഐ⭏⢙৺ަӗ૱ 伏⭘ᆹޘỰ⍻üü㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼ā䘋㹼˖ᯠ㺘䗮㳻ⲭ EPSPS ᙕᙗ∂ᙗ䈅傼Ⲵ㿲ሏᵏਚᴹ 7 ཙǄ

䈤᰾˖ᣇ㥹⭈㟖⦹㊣ MON 87427 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭоᣇ㥹⭈㟖བྷ䉶 GTS40-3-2 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭᱟаṧˈ൷ᶕⓀҾ൏༔ߌᵶ㧼 CP4 㧼ṚǄ GTS40-3-2 ᱟޘ⨳ㅜањ㻛ᢩ߶୶ъॆ⿽ἽⲴᣇ㥹⭈㟖֌⢙ˈҾ 1996 ᒤ൘㖾ഭ ᔰ࿻୶ъॆ⿽Ἵˈ䲿ਾࠐᒤⲴ⿽Ἵ㤳ത䗵䙏ᢙኅࡠे㖾৺ই㖾Ⲵ࣐᤯བྷǃ䱯ṩ ᔧǃᐤ㾯ǃᐤ᣹൝ㅹഭᇦ઼ൠ४ǄࡠⴞࡽѪ→ˈᐢ㓿୶ъॆᓄ⭘Ⲵ䖜 cp4 epsps สഐᣇ㥹⭈㟖֌⢙ᴹ 6 ⿽ˈवᤜ㤌㬯ǃ⋩㨌ǃ⦹㊣ǃỹ㣡ǃབྷ䉶઼⭌㨌˄㿱л 㺘˅Ǆ䘉Ӌ㺘䗮 CP4 EPSPS 㳻ⲭⲴᣇ㥹⭈㟖֌⢙൘ޘ⨳ᒯ⌋⿽Ἵ઼⎸䍩ˈᡀѪ ᓄ⭘䶒〟ᴰབྷⲴа㊫䖜สഐ֌⢙Ǆ

ᐢ㓿ᢩ߶ᒦ൘ޘ⨳ᒯ⌋ᓄ⭘Ⲵਜ਼ CP4 EPSPS 㳻ⲭⲴ䖜สഐ֌⢙ ᣇ㥹⭈㟖֌⢙ FDA ᢩ߶ᰦ䰤 USDA ᢩ߶ᰦ䰤㳻ⲭ⴨լᙗ* ᣇ㥹⭈㟖བྷ䉶 GTS40-3-2** 1996 1994 100% ᣇ㥹⭈㟖ỹ㣡 MON 1445** 1996 1995 100% ᣇ㥹⭈㟖⋩㨌 GT73** 1995 1999 100% ᣇ㥹⭈㟖⦹㊣ NK603** 2000 2000 100/99.9%*** ᣇ㥹⭈㟖 Flex ỹ㣡 MON 88913** 2005 2004 100% ᣇ㥹⭈㟖㤌㬯 J101 ઼ J163 2004 2005 100% ᣇ㥹⭈㟖⭌㨌 H7-1** 2004 2005 100% ᣇ㲛ᣇ㥹⭈㟖⦹㊣ MON 88017** 2005 2005 100% ᣇ㥹⭈㟖儈ӗབྷ䉶 MON 89788** 2006 2007 100% 儈⋩䞨ᣇ㥹⭈㟖བྷ䉶 MON 87705 2011 2011 100% ᣇ㥹⭈㟖⦹㊣ MON 87427 2012 2013 100% ᣇ㥹⭈㟖⋩㨌 MON 88302 2012 2013 100%

* ԕᣇ㥹⭈㟖བྷ䉶 GTS40-3-2 Ⲵ CP4 EPSPS 㳻ⲭ֌Ѫ৲➗ ** ᐢ㓿㧧ᗇѝഭᢩ߶Ⲵ䘋ਓᆹޘ䇱Җ˄⭘֌࣐ᐕ৏ᯉ˅ *** NK603 ਜ਼ᴹєњᯠ㺘䗮㳻ⲭˈCP4 EPSPS ઼ CP4 EPSPS L214Pˈє㘵ਚᴹањ≘ ส䞨Ⲵᐞ࡛

⭡Ҿ㺘䗮Ⲵ㳻ⲭ⴨਼ˈᒦфᐢ䇱ᇎἽ⢙ᶕⓀ CP4 EPSPS 㳻ⲭоབྷ㛐ᵶ㧼ᶕ Ⓚ㳻ⲭⲴㅹ਼ᙗˈ䘉Ӌ䖜สഐ֌⢙ᆹޘᙗ䇴ԧᡰ⭘Ⲵ㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼ᣕ੺ ൷䟷⭘ Hammond Ҿ 1993 ᆼᡀⲴ CP4 EPSPS 㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼ǄHammond ˄1993˅൘䈅傼ѝⲴѤᒺ㿲ሏᤱ㔝Ҷ 7 ཙˈ⭡Ҿ⋑ᴹ㿲ሏࡠн㢟৽ᓄˈㅜ 8 ཙ༴ ↫ሿ啐ᒦ䘋㹼⯵⨶ᆖ㿲ሏˈ㔃᷌ҏᵚਁ⧠ᔲᑨ˄㿱ᵜ⭣䈧Җл޼ᢰᵟᣕ੺ 10˅Ǆ

XXIX ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㳻ⲭ∂㍐а㡜ᱟ䙊䗷ᙕᙗ֌⭘ᵪࡦӗ⭏∂ᙗˈ᧕䀖ਾࠐሿᰦ㠣ࠐཙ޵ቡՊ㺘 ⧠ࠪн䘲৽ᓄˈа㡜нՊ䎵䗷аઘǄᐢ⸕ⲴἽ⢙㳻ⲭ∂㍐䜭ᱟ൘ᮠሿᰦ޵ӗ⭏∂ ᙗˈ∄ྲ˖㬆哫∂㍐ᱟ 3-24 ሿᰦǃ㓒䉶∂㍐ᱟ 6-8 ሿᰦǃἽ⢙ࠍ䳶㍐ A ѪሿҾ 3 ሿᰦǄ㘼ᐢ⸕Ⲵ㓶㧼㳻ⲭ∂㍐਼ṧՊ൘ᖸ⸝ᰦ䰤㺘⧠ࠪ∂ᙗ⯷⣦ˈྲ䴽ҡ⯵∂ Ѫ 1-5 ཙǃ㪑㨴⨳㧼㛐∂㍐ B Ѫ 1-6 ሿᰦǃ㚹∂㧼∂㍐Ѫ 18-36 ሿᰦǄHammond ˄1993˅䘋㹼Ⲵ CP4 EPSPS ሿ啐㓿ਓᙕᙗ∂ᙗ䈅傼ѝˈ♼㛳ਾ⋑ᴹࠪ⧠н㢟৽ ᓄˈ൘↔ส⹰кѤᒺ㿲ሏᤱ㔝Ҷ 7 ཙǄѤᒺ㿲ሏ㔃ᶏਾˈབྷփ䀓ࢆҏᵚ㿲ሏࡠ㜿 ಘᔲᑨǄHammond˄1993˅⹄ウѝ 7 ཙ㿲ሏᵏ⏥ⴆҶབྷཊᐢ⸕㳻ⲭ∂㍐ਁᥕ∂ ᙗⲴᴹ᭸ᰦ䰤ˈণ֯䘉ᰦ㓸→䈅傼ˈҏᓄ䈕ਟԕ⺞䇔 CP4 EPSPS 㳻ⲭ൘䈅傼ࡲ 䟿лнާᴹ∂ᙗǄ ൘ Hammond˄1993˅Ⲵ䈅傼ਾˈᆏኡ䜭ޜਨ䘈䪸ሩᣇ㥹⭈㟖⦹㊣ NK 603 ѝⲴ CP4 EPSPS 㳻ⲭ䘋㹼Ҷਖཆа亩㓿ਓᙕᙗ∂ᙗ䈅傼˄Bonnette, 2002˅Ǆ Bonnette˄2002˅䈅傼ѝⲴ CP4 EPSPS 㳻ⲭо Hammond˄1993˅ᡰ⭘㳻ⲭӵ⴨ ᐞањ≘ส䞨ˈ਼Ⓚᙗ䗮ࡠ 99.9%˄㿱к㺘˅ǄBonnette˄2002˅൘䈅傼ѝ䟷⭘ Ⲵ㳻ⲭ♼㛳ࡲ䟿Ѫ 817 mg/kgˈѤᒺ㿲ሏᵏѪ 14 ཙˈ⋑ᴹ㿲ሏࡠ CP4 EPSPS ൘ 䈅傼ᴰ儈ࡲ䟿≤ᒣлާᴹн㢟᭸ᓄˈ䈅傼ᡰᗇ㔃䇪о Hammond˄1993˅㔃䇪а ṧǄBonnette˄2002˅Ⲵ䈅傼ᣕ੺ᐢ䲿ᣇ㥹⭈㟖⦹㊣ NK 603 Ⲵᆹޘ䇴ԧ⭣䈧䙂 Ӕࡠޘ⨳਴ഭᇦⲴ⌅㿴ⴁ㇑ᵪᶴˈᒦ㻛ᇑḕ઼ᢩ߶Ǆ ਨሩ MON 87427 䖜สഐޜ䇶᮷Ԧ઼∂⨶ᆖ⹄ウᜟֻˈᆏኡ䜭ޡ 䚥ᗚ Codex ∄㊣ѝᯠ㺘䗮㳻ⲭ CP4 EPSPS 䘋㹼Ҷ˖㔃ᶴ⍫ᙗ࠶᷀˄਼ⓀᙗⲴ⭏⢙ؑ᚟ᆖ⦹ ⭘শਢ˅ǃփཆ⍻䈅˄✝っᇊᙗ઼ SGF ⎸ॆっᇊ֯ޘሩ˅ǃ⍱㹼⯵ᆖ࠶᷀˄ᆹ ᙗ˅ˈԕ৺ࣘ⢙⭏⍻˄ԕ㓟ॆ㳻ⲭ䘋㹼ሿ啐ᙕᙗ∂ᙗ⹄ウ˅Ǆ䘉Ӌ࠶᷀䍴ᯉҏ㺘 ᰾ CP4 EPSPS нՊ㺘⧠ࠪᔦ䘏∂ᙗ˄䈖㿱ᵜ⭣䈧Җѝㅜ 3.3 䜘࠶˅˖ ˗⭏⢙ؑ᚟ᆖ࠶᷀㺘᰾ˈCP4 EPSPS оᐢ⸕Ⲵ∂㍐㳻ⲭнާᴹ਼Ⓚᙗ˅6 7˅CP4 EPSPS оӪ㊫઼ࣘ⢙ᰕᑨ伏⭘Ⲵᒦᐢ䇱᰾ᱟᆹޘⲴἽ⢙㳻ⲭ˄ྲἽ ⢙޵Ⓚ EPSPS 㳻ⲭ˅ᴹᖸ儈਼Ⓚᙗˈ㘼䘉Ӌ㳻ⲭ൷ާᴹ䮯ᵏᆹޘ伏⭘শਢ˗ 8˅փཆ⎸ॆ䈅傼㺘᰾ˈCP4 EPSPS 㳻ⲭ൘⁑ᤏ㛳⏢˄SGF˅ѝՊ㻛䗵䙏⎸ ॆᦹˈнާᴹ㌫㔏ᙗ᳤䵢䰞仈Ǆ 9˅CP4 EPSPS 㳻ⲭሩ✝нっᇊˈ㘼ᰕᑨ㟣伏ѝ⦹㊣䙊ᑨ䴰㾱✩ࡦˈ߿ቁҶ ާᴹᆼᮤ࣏㜭 CP4 EPSPS 㳻ⲭ൘㟣伏ѝⲴ᳤䵢ࠐ⦷˄Hammond and Jez, 2011˅Ǆ䈅傼㔃᷌㺘᰾ˈCP4 EPSPS 㳻ⲭ൘⑙ᓖ䎵䗷 75°C ᰦ࣏㜭⍫ᙗᆼޘ їཡǄ 㘳㲁ࡠབྷཊᮠ㳻ⲭ䜭нާᴹ∂ᙗǃCP4 EPSPS 㳻ⲭާᴹᆹޘ⎸䍩শਢԕ৺ᣇ MOE˅䜭ᖸབྷ˄MON 87427 ⦹㊣ѝ CP4 EPSPS˄㥹⭈㟖֌⢙ѝ䈕㳻ⲭⲴ᳤䵢䲀٬ 㳻ⲭሩѝഭӪ㗔㘼䀰ަ MOE Ѫ㠣ቁ 1.4 × 104 㠣 7.9 × 105ˈ㿱ᵜ⭣䈧Җㅜ 102-104 亥Ӫ㗔㟣伏ᩴޕ仾䲙䇴ՠ˅ˈᡰԕᴹ⨶⭡ᶕ᧘ᯝ伏⢙઼侢ᯉѝⲴ CP4 EPSPS н ՊሩӪ㊫઼ࣘ⢙䙐ᡀডᇣǄቡ MON 87427 ⦹㊣ѝⲴ CP4 EPSPS 㳻ⲭ㘼䀰ˈањ փ䟽Ѫ 50 kg ⲴӪ䴰㾱⇿ཙ⎸䍩㓖 7900 kg˄䎵䗷㠚䓛փ䟽 150 ؽ˅Ⲵ MON 87427

XXX ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⦹㊣㊭㋂᡽㜭䗮ࡠо Hammond˄1993˅ሿ啐ᙕᙗ∂ᙗ⹄ウᡰ⭘ࡲ䟿བྷ㠤⴨ᖃⲴ CP4 EPSPS 㳻ⲭ᳤䵢≤ᒣǄ ↔ཆˈCP4 EPSPS 㳻ⲭާᴹ൘ޘ⨳伏⢙䬮ѝ䮯ᵏᆹޘ⎸䍩ⲴশਢǄ㠚Ӿ 1996 ᒤᣇ㥹⭈㟖བྷ䉶୶ъॆ⿽Ἵԕᶕˈਜ਼ᴹ CP4 EPSPS 㳻ⲭⲴߌӗ૱ቡаⴤ൘ޘ⨳ ⢙伏⭘Ǆਜ਼ᴹ CP4 EPSPS Ⲵᣇ㥹⭈㟖֌ޘ伏⢙׋ᓄ䬮ѝᆈ൘ᒦ㻛Ӫ㊫઼ࣘ⢙ᆹ ⇿ᒤ䜭ᴹ 30 ཊњӗ૱൘ 13 њഭᇦ⿽Ἵ䶒〟䎵䗷 7400 зޜ产˄Dill et al., 2008˅Ǆ 㠚 1996 ᒤᣇ㥹⭈㟖֌⢙୶ъॆ⿽Ἵԕᶕˈᐢᴹ䎵䗷 1000 ӯཤࣘ⢙㻛侢லਜ਼ᴹ䖜 ᓧ઼⭏ӗ⦷Ⲵᣕ੺Ǆڕ⢙สഐ֌⢙˄བྷ䜘࠶ᱟᣇ㥹⭈㟖֌⢙˅Ⲵ侢ᯉˈ⋑ᴹᖡ૽ࣘ аㇷ㓿䗷਼㹼䇴䇞ⲴޣҾ侢லਜ਼ᴹ䖜สഐ֌⢙˄वᤜᣇ㥹⭈㟖֌⢙˅侢ᯉሩࣘ⢙ ᓧᖡ૽Ⲵ᮷⥞㔬䘠˄Eenennaam & Young, 2014˅ҏᤷࠪˈⴞࡽ䘈⋑ᴹڕ⭏䮯઼ ᓧӗ⭏н࡙ᖡ૽Ǆ൘䗷৫ڕԫօֻᆀ㺘᰾ਜ਼ᴹ䖜สഐ֌⢙侢ᯉՊሩࣘ⢙⭏䮯઼ 20 ᒤˈޘ⨳ᐢ㓿ᴹ 488 亩⅑ޣҾ CP4 EPSPS 㳻ⲭᆹޘᙗⲴᇑḕᡆ䇴ᇑˈᗇࠪⲴ ᓧ⋑ᴹн㢟ᖡ૽Ǆڕ⢙㔃䇪൷䇔Ѫ CP4 EPSPS 㳻ⲭሩӪ㊫઼ࣘ

৲㘳᮷⥞ Codex Alimentarius. 2003. Guideline for the conduct of food safety assessment of foods derived from recombinant-DNA plants. CAC/GL 45-2003. Codex Alimentarius Comission, Joint FAO/WHO Food Standards Programme, Food and Agriculture Organisation, Rome, Italy Codex Alimentarius. 2009. Foods derived from modern biotechnology. Second Edition, World Health Organization, Food and Agriculture Organization of the United Nations, Rome, Italy. Dill, G.M., C.A. CaJacob and S.R. Padgette. 2008. Glyphosate-resistant crops: Adoption, use and future considerations. Pest Management Science 64:326-331. Van Eenennaam, A.L. 2014. Prevalence and impacts of genetically engineered feedstuffs on livestock populations. Journal of Animal Science 92:4255-4278.

XXXI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䰞仈 2˖ ᵚᨀ׋ᯠ㺘䗮㳻ⲭ EPSPS 䞦⍫ᙗǃ䞦⍫ᙗᖡ૽ഐ㍐˄ྲ pHǃ⑙ᓖǃ ⿫ᆀᕪᓖ˅ǃᓅ⢙⢩ᔲᙗ઼৽ᓄӗ⢙ㅹ䍴ᯉǄ

䈤᰾˖5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦˄EPSPS˅ᒯ⌋ᆈ൘ҾἽ⢙઼ᗞ⭏⢙ փ޵ˈᱟ㧭㥹䞨䙄ᖴѝⲴањޣ䭞䞦ˈ㘼㧭㥹䞨䙄ᖴ䍏䍓൘Ἵ⢙઼ᗞ⭏⢙փ޵ ⢙ᨀ׋㣣俉⧟ˈ⭘Ҿ㣣俉᯿≘ส䞨˄㤟щ≘䞨ǃ䞚≘䞨ǃ㢢≘䞨˅઼䇨ཊ⅑㓗 ˄ྲഋ≒ਦ䞨ǃ⌋䞼ǃ㔤⭏㍐ Kǃ㣡䶂㍐ǃ㊫哴䞞ǃᵘ䍘㍐ㅹ˅Ⲵ⭏⢙ਸᡀ ˄Herrmann and Weaver 1999; Maeda and Dudareva 2012˅Ǆ䈕䙊䐟൘Ἵ⢙փ޵䶎 ᑨ䟽㾱ˈἽ⢙փ㠣ቁ 35%Ⲵᒢ⢙䍘ᶕⓀҾ㧭㥹䞨䙄ᖴⲴӗ⢙˄Franz et al. 1997˅Ǆ лമᡰ⽪ˈEPSPS 䞦֌⭘Ҿ㧭㥹䞨䙄ᖴѝⲴㅜ 6 ↕ˈ䍏䍓ۜॆሶ⼧䞨✟䞷ྲ ᔿщ䞞䞨˄PEP˅кⲴ✟䞷ᔿщ䞞䞠ส䖜〫ࡠ㧭㥹䞨-3-⼧䞨˄S3P˅кˈ৽ᓄ⭏ 2- ᡀ 5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨˄EPSP˅ˈ਼ᰦ䟺᭮ࠪᰐᵪ⼧䞨˄HPO4 ˅Ǆ ˄Alibhai and Stallings, 2001˅Ǆ㘼ߌ䗮®䲔㥹ࡲѝⲴᴹ᭸ᡀ࠶㥹⭈㟖˄glyphosate˅ ᱟ PEP Ⲵㄎҹᙗᣁࡦ⢙ˈ㥹⭈㟖Ⲵᆈ൘ਟ䱫→ EPSPS 䞦ሩ PEP Ⲵ࡙⭘ˈ䱫ᯝ㣣䭞ԓ䉒ӗ⢙ˈӾ㘼ਁᥕ䲔㥹֌⭘Ǆޣ俉⧟Ⲵਸᡀˈ֯ᗇἽ⢙㕪ѿབྷ䟿⭏䮯ᡰ䴰Ⲵ 儈ㅹ⭏⢙ྲପңࣘ⢙փ޵нᆈ൘㧭㥹䞨䙄ᖴˈਚ㜭Ӿ伏⢙ѝ㧧ਆ㣣俉᯿≘ส䞨˗ ⭡Ҿ㠚䓛н㜭ਸᡀ EPSPS 䞦ˈପңࣘ⢙ሩ㥹⭈㟖н᭿ᝏǄ

PEP

Inhibitor:

Shikimate-3-phosphate Glyphosate 5-Enolpyruvyl-shikimate-3-phosphate S3P EPSP

CP4 EPSPS ᴰࡍᱟӾ൏༔ѝᑨ㿱Ⲵߌᵶ㧼˄Agrobacterium˅CP4 㧼Ṛѝ࠶⿫ ᗇࡠⲴǄ⭡Ҿަۜॆս⛩Ⲵ㔃ᶴ⢩ᖱˈCP4 EPSPS ሩ㥹⭈㟖Ⲵᣁࡦާᴹཙ❦Ⲵн ᭿ᝏᙗ˄Funke, et al. 2006˅Ǆᣇ㥹⭈㟖֌⢙ѝⲴ CP4 EPSPS 䙊ᑨ൘ N-ᵛㄟᑖᴹ а⇥ਦ㔯փ䖜䘀㛭ˈ⭘Ҿᕅሬ CP4 EPSPS ᇊսࡠਦ㔯փкˈ䘉ᱟ޵ⓀᙗἽ⢙ EPSPS 䞦൘㓶㜎޵Ⲵս㖞Ǆ CP4 EPSPS ᱟ⭡ 455 њ≘ส䞨㓴ᡀⲴঅ䬮ཊ㛭ˈ⨶䇪࠶ᆀ䟿Ѫ 47.6 kDaǄӾ ᒿࡇкⴻˈCP4 EPSPS оἽ⢙ᶕⓀⲴ EPSPS ާᴹ 25-30%Ⲵ⴨լᙗˈо㓶㧼ᶕⓀ Ⲵ EPSPS ާᴹ 50-90%Ⲵ⴨լᙗǄ㲭❦൘㳻ⲭ䍘а㓗㔃ᶴк਴㊫ EPSPS Ⲵ≘ส䞨 ᒿࡇᒦнॱ࠶⴨լˈն EPSPS 㳻ⲭᇦ᯿൘䞦⍫ᙗѝᗳ઼й㓗㔃ᶴⲴޣ䭞ս⛩к ᆸˈഐ↔ⴞࡽᐢ⸕ⲴᡰᴹᶕⓀ EPSPS 䞦˄वᤜ CP4 EPSPS˅൘й؍Ⲵᒿࡇ䶎ᑨ 㓗ᤃᢁ㔃ᶴк儈ᓖа㠤˄Hammond, et al. 2013˅˄മ 2˅ǄӾᇊѹкᶕ䈤ˈ਼Ⓚ㳻 ⲭᴹ⴨լⲴҼ㓗㔃ᶴ઼й㓗ᶴ䊑ˈӾ㘼ާᴹ⴨լⲴ࣏㜭˄Pearson, 2000˅Ǆ CP4 EPSPS ԕঅփᖒᔿᢗ㹼ۜॆ࣏㜭ˈн䴰㾱䖵䞦ᡆ⢩↺䠁኎⿫ᆀ䖵ࣙǄ

XXXII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ѫᵚоᓅ⢙㔃ਸⲴ CP4 EPSPSחCP4 EPSPS Ⲵй㓗Ღփ㔃ᶴྲлമᡰ⽪ˈA മᐖ Ѫоᓅ⢙ S3P 㔃ਸਾⲴ䰝ਸ⣦ᘱⲴ䞦Ǆоަᆳח䞦ˈԕᔰ᭮⣦ᘱⲴᶴ䊑ᆈ൘˗ਣ EPSPS аṧˈCP4 EPSPS 䞦⭡є䜘࠶ॺ⨳⣦㔃ᶴฏ䙊䗷䬠䬮४䘎᧕ˈ⇿њॺ⨳ 㔃ᶴฏ൷⭡ཊњᒣ㹼Ⲵ-㷪᯻㓴ᡀˈ䞦ۜॆ⍫ᙗս⛩սҾєњॺ⨳⣦㔃ᶴฏѻ⣦ ⢙䰤ˈ⍫ᙗս⛩䱴䘁ਜ਼ᴹ䖳ཊᑖ⭥㦧Ⲵ≘ส䞨↻สˈׯҾоᓅ⢙⴨ӂ֌⭘Ǆоᓅ S3P Ⲵ㔃ਸ䈡ሬ䞦Ⲵᶴ䊑ਁ⭏ࢗ⛸ਈॆˈ৏ᵜ੸Āᢃᔰā⣦ᘱⲴєњॺ⨳⣦㔃ᶴ ฏ䰝ਸˈׯҾ䞦઼ PEP Ⲵ㔃ਸ˄ྲ B മᡰ⽪˅ǄCP4 EPSPS ሩ㥹⭈㟖Ⲵ㙀ਇ↓ᱟ ⭡Ҿަ⢩↺Ⲵࠐօᆖᶴ䙐⢩⛩˄Funke, et al. 2006˅ǄA മѝ₉㢢⧟⣦㔃ᶴᱟ䶐䘁 ᆸ↻ส Glu-354؍ᴹⲴ儈ᓖޡC-ᵛㄟⲴㅜ 347-358 ս↻สˈवᤜ EPSPS 䞦ᇦ᯿ᡰ ઼ Arg-357ˈо PEP/㥹⭈㟖Ⲵ㔃ਸᴹޣǄ⢩࡛䟽㾱Ⲵᱟ CP4 EPSPS ㅜ 100 սк 䬮䱫→Ҷ㥹⭈㟖о䞦Ⲵ㔃חⲴщ≘䞨↻ส˄㿱 B മѝ A100˅Ǆщ≘䞨кⲴ⭢ส ਸ˄Funke, et al. 2006˅ǄӪѪሶㅜ 100 սкⲴщ≘䞨ケਈᡀ⭈≘䞨ਾˈケਈⲴ CP4 EPSPS 䞦ሩ㥹⭈㟖ਈᗇ᭿ᝏǄѻࡽҏᴹ⹄ウ㺘᰾ˈབྷ㛐ᵶ㧼 EPSPS ѝ⴨ᓄ ս⛩ྲ᷌Ӿ⭈≘䞨ケਈᡀщ≘䞨˄Gly96Ala˅ˈ㜭ཏབྷབྷ䱽վަሩ㥹⭈㟖Ⲵ᭿ᝏ ᙗˈն਼ᰦо PEP ⲴӢ઼ᙗҏབྷབྷл䱽˄Barry et al. 1992; Padgette et al. 1996˅Ǆ ᤱ؍ CP4 EPSPS 䞦Ⲵ⤜⢩ᶴ䙐֯ᗇަሩ㥹⭈㟖н᭿ᝏˈն਼ᰦሩཙ❦ᓅ⢙ PEP ᖸ儈ⲴӢ઼ᙗǄ EPSPS 䞦˄वᤜ CP4 EPSPS˅ާᴹ儈ᓖⲴᓅ⢙⢩ᔲᙗǄⴞࡽᐢ⸕ሩ EPSPS ਈॆǄ׳䞦ާᴹ⭏⢙ᆖ᜿ѹⲴᓅ⢙ӵѪ S3P ઼ PEPǄ㥹⭈㟖н㜭ਁ⭏ EPSPS 䞦㧭㥹䞨оབྷ㛐ᵶ㧼ᶕⓀ EPSPS 䞦Ⲵ㔃ਸ㜭࣋䶎ᑨᕡˈо S3P ⴨∄ˈ㧭㥹䞨䴰㾱 ˈ䶎ᑨ儈⎃ᓖ˄mM 㓗˅᡽ᴹਟ㜭㻛 EPSPS 䞦ۜॆ˄Gruys, et al. 1992˅Ǆ⹄ウ㺘᰾ Ἵ⢙փ޵ཙ❦ᆈ൘ S3P ㊫լ⢙ྲ⭢ส㧭㥹䞨ǃཾቬ䞨ǃҼ≒㧭㥹䞨䜭нᱟ EPSPS Ⲵᓅ⢙˄Franz, et al. 1997˅Ǆ ٬ǃ⑙ᓖ઼⿫ᆀᕪᓖㅹഐ㍐ᖡ૽Ǆ CP4 EPSPS 㳻ⲭⲴ䞦⍫ᙗ઼っᇊᙗਇ pH ٬䗷儈ᡆ䗷վ䜭Պ䱽վ䞦⍫ᙗˈpH ሿҾ 5.5 ᰦ ᴰ䘲䞦⍫ pH Ѫ 9-9.5ˈpH CP4 EPSPS 䞦⍫ࠐѾᆼޘїཡˈн䗷⭡ pH ᕅ䎧Ⲵ䞦⍫ਈॆᒦ䶎нਟ䘶˄Padgette et al. 1993˅ǄCP4 EPSPS ሩ✝нっᇊˈ⑙ᓖ䎵䗷 75°C ᰦˈCP4 EPSPS Ⲵ࣏㜭⍫ ᙗণ䱽㠣Ự⍻䲀ԕлˈն࣐✝ᒦнᖡ૽㳻ⲭа㓗㔃ᶴⲴᆼᮤᙗ˄䈖㿱ᵜ⭣䈧Җѝ ㅜ 3.3 䜘࠶˅Ǆ䘉ㅖਸ✝ࣘ࣋ᆖ⌅ࡉˈণ⑙ᓖॷ儈Պ֯㳻ⲭ儈㓗㔃ᶴਈᙗˈᕅ䎧䞦⍫ᙗїཡǄCP4 EPSPS ሩ✝нっᇊⲴ⢩ᙗ㺘᰾ˈ൘ᐕъ࣐ᐕᶑԦлˈᴰ㓸Ⲵ䖜 สഐ⦹㊣ӗ૱ѝнཚਟ㜭ᆈ൘ާᴹ࣏㜭⍫ᙗⲴ CP4 EPSPS 㳻ⲭ˄Hammond and Jez 2011˅ǄоᡰᴹިරⲴ㳻ⲭ䞦㊫аṧˈCP4 EPSPS Ⲵۜॆ⍫ᙗ䘈ਇ৽ᓄⓦ⏢ѝ ⿫ᆀᕪᓖⲴᖡ૽Ǆ↓ᑨⲴ䍞ᆈ㕃ߢ⏢˄1mMKCl˅ᶑԦлˈCP4 EPSPS Ⲵ⢩ᔲ ⍫ᙗᴰ儈㜭ཏ䗮ࡠ 5.5 U/mgǄCP4 EPSPS Ⲵ⢩ᔲ⍫ᙗ䲿⵰⿫ᆀᕪᓖ໎བྷ㘼ॷ儈ˈ 㠣 50 mM KCl ᰦˈ䞦⍫ᙗ㜭ཏ໎࣐ 2-2.6 ؽ˄Padgette et al. 1993˅Ǆ

XXXIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ৲㘳᮷⥞ Alibhai, M.F. and W.C. Stallings. 2001. Closing down on glyphosate inhibition - With a new structure for drug discovery. Proceedings of the National Academy of Sciences of the United States of America 98:2944-2946. Barry, G., G. Kishore, S. Padgette, M. Taylor, K. Kolacz, M. Weldon, D. Re, D. Eichholtz, K. Fincher and L. Hallas. 1992. Inhibitors of Amino Acid Biosynthesis: Strategies for Imparting Glyphosate Tolerance to Crop Plants. Pages 139-145 in Biosynthesis and molecular Regulation of Amino Acids in Plants B.K. Singh, H.E. Flores, and Shannon (eds.). American Soceity of Plant Physiologists. Franz, J.E., M.K. Mao and J.A. Sikorski. 1997. Glyphosate's Molecular Mode of Action. Glyphosate A Unique Global herbicide. American Chemical Society, Washington, DC.

XXXIV ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Funke, T., H. Han, M.L. Healy-Fried, M. Fischer and E. Schonbrunn. 2006. Molecular basis for the herbicide resistance of Roundup Ready crops. Proc Natl Acad Sci U S A 103:13010-13015. Gruys, K.J., M.C. Walker and J.A. Sikorski. 1992. Substrate synergism and the steady-state kinetic reaction mechanism for EPSP synthase from Escherichia coli.Biochemistry 31:5534-5544. Hammond, B., J. Kough, C. Herouet-Guicheney, J.M. Jez. 2013. Toxicological evaluation of proteins introduced into food crops. Crit Rev Toxicol 43 Suppl 2:25-42. Hammond, B.G. and J.M. Jez. 2011. Impact of food processing on the safety assessment for proteins introduced into biotechnology-derived soybean and corn crops. Food Chem Toxicol 49:711-721. Herrmann, K.M. and L.M. Weaver. 1999. The Shikimate Pathway. Annual revel of Plant Physiology and Plant Molecular Biology 50:473-503. Kishore, G.M. and D.M. Shah. 1988. Amino acid biosynthesis inhibitors as herbicides. Annu Rev Biochem 57:627-663. Maeda, H. and N. Dudareva. 2012. The shikimate pathway and aromatic amino Acid biosynthesis in plants. Annu Rev Plant Biol 63:73-105. Padgette, S.R., G.F. Barry, D.B. Re, D.A. Eichholtz, M. Weldon, K. Kolacz and G.M. Kishore. 1993. Purification, Cloning, and Characterization of a Highly Glyphosate-tolerant 5-Enolpyruvylshikimate-3-phosphate Synthase from Agrobacterium sp. strain CP4. Monsanto Technical Report (St. Louis) MSL-12738. Padgette, S.R., D.B. Re, G.F. Barry, D.E. Eichholtz, X. Delannay, R.L. Fuchs, G.M. Kishore and R.T. Fraley. 1996. New weed control opportunities: Development of soybeans with a Roundup ReadyTM gene. Pages 53-84 in Herbicide-Resistant Crops: Agricultural, Environmental, Economic, Regulatory, and Technical Aspects. S.O. Duke (ed.). CRC Press, Boca Raton, Florida. Pearson, W.R. 2000. Flexible sequence similarity searching with the FASTA3 program package. Methods in Molecular Biology 132:185-219.

XXXV ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ӗ૱ѝ䲔㥹ࡲ㥹⭈㟖ޣ䰞仈 3˖ᵚᨀ׋⦹㊣⿽ᆀǃ⦹㊣ǃ⦹㊣⋩ǃ⦹㊣㊹ㅹ⴨ Ⲵ↻⮉䟿ᮠᦞǄ 䈤᰾˖㥹⭈㟖ᱟа⿽䶎䘹ᤙᙗᒯ䉡䲔㥹ࡲˈ㜭ཏᣁࡦἽ⢙޵Ⓚ 5-✟䞷ᔿ㧭㥹䞨щ 䞞䞠-3-⼧䞨ਸ䞦˄EPSPS˅Ⲵ⍫ᙗˈӾ㘼䱫ᯝἽ⢙⭏䮯ᡰᗵ䴰Ⲵ㣣俉᯿≘ส䞨Ⲵ ⭏⢙ਸᡀˈሬ㠤Ἵ⢙↫ӑˈ䗮ࡠ䲔㥹ⴞⲴǄᣇ㥹⭈㟖㊫䖜สഐ֌⢙࡙⭘Ҷа⿽ሩ 㥹⭈㟖н᭿ᝏⲴ EPSPS 䞦˄⭡ҾᶕⓀҾ൏༔ߌᵶ㧼 CP4 㧼Ṛˈભ਽Ѫ CP4 EPSPS˅ˈ ޵Ⓚᙗ EPSPS Ⲵ࣏㜭ˈ֯֌⢙⭏䮯нਇ㥹⭈㟖ᣁࡦǄگ൘௧ᯭ㥹⭈㟖ਾˈ㜭ཏԓ ൘ᯠⲴᣇ䲔㥹ࡲᙗ⣦୶ъॆ⿽ἽѻࡽˈՊ᤹➗㢟ྭߌъ㿴㤳˄Good Agricultural Practices ˈGAP˅ḷ߶䘋㹼⭠䰤䈅傼ˈ᤹➗᧘㦀ࡲ䟿઼ᯩ⌅൘֌ ⢙кⴤ᧕௧ᯭ䲔㥹ࡲˈ࠶᷀⺞ᇊⴤ᧕᭦㧧Ⲵߌӗ૱৺ަ࣐ᐕ㓴࠶ѝⲴ䲔㥹ࡲ↻ ⮉≤ᒣǄ䲔㥹ࡲ൘㊭㋂н਼㓴࠶ѝ↻⮉≤ᒣਟ㜭ᆈ൘ᐞᔲˈ൘ḀӋ㓴࠶ѝ⴨ሩ ᇼ䳶ˈ㘼൘ަᆳ㓴࠶ѝ⴨ሩ䖳ቁǄ࣐ᐕ㓴࠶ѝⲴ⴨ሩ↻⮉⎃㕙≤ᒣ⭘⎃㕙㌫ᮠ ˄concentration factors, CF˅㺘⽪ˈ䇑㇇ޜᔿྲл˖CF=࣐ᐕ㓴࠶↻⮉≤ᒣ/ᵚ ࣐ᐕ㊭㋂↻⮉≤ᒣǄ 䪸ሩ俆њᣇ⢩ᇊ䲔㥹ࡲᙗ⣦ᔰਁⲴ↻⮉ᮠᦞਟԕ֌Ѫ䈕㊫ӗ૱Ⲵส⹰ᮠ ᦞˈਾ㔝ᔰਁⲴᣇ⴨਼䲔㥹ࡲⲴӗ૱ਚ㾱ᕅޕ㳻ⲭⲴ֌⭘ᵪࡦ⴨਼ˈ∄ྲ䜭ᱟ ᣇ㥹⭈㟖Ⲵ EPSPS 㳻ⲭˈᒦ᤹➗⴨਼ḷㆮ᧘㦀Ⲵ௧ᯭࡲ䟿઼ᯩ⌅֯⭘䲔ޕᕅ 㥹ࡲˈᴰࡍᔰਁⲴ↻⮉ส⹰ᮠᦞਟ⭘Ҿᯠ䖜ॆһԦӗ૱Ⲵ↻⮉䟿䇴ՠǄሩҾᣇ 䲔㥹ࡲ⦹㊣ᶕ䈤ˈสҾㅜањᣇ㥹⭈㟖⦹㊣ GA21 ᔰਁⲴ↻⮉ᮠᦞवᤜ⦹㊣㊭㋂઼㊭㋂࣐ᐕ㓴࠶⦹㊣⋩઼⦹㊣㊹˄㿱л㺘˅ˈ䘉Ӌ㓴࠶สᵜ⏥ⴆҶ⦹㊣⭘֌ ࣐ᐕ৏ᯉⲴ䘋ਓӗ૱Ǆ⿽ᆀ઼㊭㋂൘ᾲᘥкᱟਟԕӂᦒⲴˈն⦹㊣ᵲӔ⿽ᆀⲴ ⴞⲴᱟ⭘ᶕ⿽Ἵˈ䙊ᑨнՊ⭘ҾӪ㊫⎸䍩ˈ亴䇑⦹㊣⿽ᆀⲴ↻⮉䟿亴䇑઼㊭㋂ ⴨լǄ䘉Ӌ↻⮉ส⹰ᮠᦞ䘲⭘Ҿᣇ㥹⭈㟖⦹㊣ MON 87427×NK603 ǃ MON 87427 × MON 88017 ㅹ਼㊫ӗ૱Ǆ MON 87427 Ⲵ䳴ᙗಘᇈѝ CP4 EPSPS 㳻ⲭ㺘䗮䟿ᶱվˈн㜭㙀ਇ㥹⭈㟖䲔㥹ࡲǄ൘⦹㊣ᵲӔ⿽ࡦ⿽䗷〻ѝˈ⭘ਜ਼ᴹ MON 87427 䖜ॆһԦⲴ⦹㊣㠚Ӕ ㌫֌⇽ᵜˈُࣙҾ㥹⭈㟖䲔㥹ࡲⲴ௧ᯭӗ⭏䳴ᙗн㛢ˈ⭘ਜ਼ᴹ NK603ǃ MON 88017 ㅹަᆳᣇ䲔㥹ࡲ䖜ॆһԦⲴ㠚Ӕ㌫֌⡦ᵜˈ⭏ӗⲴᵲӔ⿽ MON 87427 × NK603 ᡆ MON 87427 × MON 88017 ⭘Ҿ⭏ӗ䬰୞Ǆ䘉ӋᵲӔ⿽ Ⲵṭษ䗷〻ѝˈߌ≁׍❦Պ᤹➗⴨਼Ⲵ㥹⭈㟖ḷㆮ᧘㦀ᯩᔿ௧ᯭ䲔㥹ࡲǄ 2008 ᒤ൘㖾ഭ⦹㊣⿽Ἵ४ 6 њᐎǃ6 њ䈅傼⛩ሩਜ਼ᴹ MON 87427 䖜ॆһԦ Ⲵ⦹㊣ᵲӔ⿽ MON 87427 × NK603 ઼ MON 87427 × MON 88017 䘋㹼⭠䰤䈅傼ˈ ➗ḷㆮ᧘㦀֯⭘ᯩ⌅௧ᯭ㥹⭈㟖ˈỰ⍻࠶᷀Ҷ᭦㧧Ⲵ⦹㊣㊭㋂ѝ㥹⭈㟖৺ަԓ᤹ 䉒ӗ⢙≘⭢ส⼧䞨˄AMPA˅Ⲵ↻⮉≤ᒣ˄㿱ᢰᵟᣕ੺ 14˅ˈഭ䱵伏૱⌅ިင ઈՊ˄Codex˅൘⦹㊣кᡰ䇮ᇊⲴ㥹⭈㟖ᴰབྷ↻⮉䲀䟿˄MRL˅ਚवᤜ㥹⭈㟖Ǆ к䘠 MON 87427 ᵲӔ⿽㥹⭈㟖ⲴỰ⍻㔃᷌㿱л㺘ˈ㺘ѝ MON 87427 ᵲӔ⿽⦹ ㊣㥹⭈㟖↻⮉䟿ⲴỰ⍻㔃᷌о࡙⭘ㅜањᣇ㥹⭈㟖⦹㊣ GA21 ᔪ・Ⲵส⹰ᮠᦞ ・٬Ǆ㘼สҾ GA21 䘋㹼Ⲵ࣐ᐕ㓴࠶↻⮉⹄ウᔪ สᵜа㠤ˈ䜭վҾ Codex MRL Ҷ䈕㊫⦹㊣⋩ǃ⦹㊣㊹Ⲵ↻⮉ᮠᦞˈࡇҾл㺘Ǆ

XXXVI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᣇ㥹⭈㟖⦹㊣↻⮉䈅傼ᙫ㔃৺ Codex ᴰབྷ↻⮉䲀䟿≤ᒣ˄MRL˅ MON 87427 × MON 87427 × 㥹⭈㟖 GA21 ᵲӔ⿽ 1 NK603 ᵲӔ⿽ 2 MON 88017 ᵲӔ⿽ 2 㓴㓷㊫ර Codex MRL ѝսᮠ˄㤳ത˅ ѝսᮠ˄㤳ത˅ ѝսᮠ˄㤳ത˅ ppm ppm ppm ppm 0.11 0.04 0.06 ⦹㊣㊭㋂ 5 (<0.05 - 0.96) (0.01 - 0.13) (0.01 – 0.20) <0.04 ㋮⛬⦹㊣⋩ ᵚ䇮ᇊ ᵚ⍻ᇊ ᵚ⍻ᇊ (<0.02 – 0.32)3 0.12 ⦹㊣㊹ ᵚ䇮ᇊ ᵚ⍻ᇊ ᵚ⍻ᇊ (<0.05 – 1.1)3 1 ਜ਼ᴹ䖜ॆһԦ GA21 Ⲵㅜаԓᣇ㥹⭈㟖⦹㊣ᵲӔ⿽Ⲵ㥹⭈㟖↻⮉ᮠᦞˈ⭘Ҿᔪ・䈕㊫ᣇ㥹⭈㟖⦹㊣᭦㧧ӗ૱઼᭦㧧㊭㋂࣐ᐕ㓴࠶↻⮉ส⹰ᮠᦞ˗ 2 ᮠᦞᶕ㠚 MON 87427 о NK603 ᡆ MON 88017 ↻⮉䈅傼˄ᢰᵟᣕ੺ 14˅ ˅࣐ᐕ㓴࠶ѝⲴ↻⮉≤ᒣѝսᮠ઼㤳ത׍ᦞ㊭㋂↻⮉≤ᒣ઼⴨ᓄ㓴࠶⎃㕙ഐᆀ˄CF 3 䇑㇇ᗇࡠǄަѝ⦹㊣⋩ CF<0.33ˈ⦹㊣㊹ CF Ѫ 1.1Ǆ к㺘ѝഭ䱵伏૱⌅ިငઈՊ˄Codex˅ᡰ䇮・Ⲵᴰབྷ↻⮉䲀䟿ᴰࡍᱟ൘ᑨ 㿴⦹㊣к䇮ᇊⲴǄ൘ᣇ㥹⭈㟖⦹㊣кⲴ↻⮉䈅傼㔃᷌㺘᰾ˈ䲔㥹ࡲ㥹⭈㟖Ⲵ↻ ⮉≤ᒣվҾ Codex 䇮ᇊⲴ MRLǄഐ↔⧠ᴹⲴ Codex MRL ᰒ䘲⭘Ҿᑨ㿴⦹㊣ˈ ҏ䘲⭘Ҿᣇ㥹⭈㟖䲔㥹ࡲ⦹㊣Ǆྲ᷌⦹㊣࣐ᐕ㓴࠶Ự⍻ࡠⲴ↻⮉䟿≤ᒣ᰾ᱮ儈 ٬Ǆն Codex ᡆ䇨Պ䪸ሩ࣐ᐕ㓴࠶䇮ᇊу䰘Ⲵ MRLˈ٬ Ҿ㊭㋂к䇮ᇊⲴ MRL ⭡Ҿ⦹㊣⋩ǃ⦹㊣㊹ㅹ㓴࠶ѝỰ⍻ࡠⲴ㥹⭈㟖Ⲵ↻⮉䟿䘌վҾ㊭㋂кⲴ MRL ٬Ǆ ppm˅ˈഐ↔⋑ᴹу䰘䇮・⦹㊣㊹ǃ⦹㊣⋩Ⲵ MRL 5˄٬ ߌ㦟ᴰབྷ↻⮉䲀䟿˄MRL˅ᱟᤷ伏૱ᡆߌӗ૱޵䜘ᡆ㺘䶒⌅ᇊݱ䇨Ⲵߌ㦟 ᴰབྷ⎃ᓖˈԕ⇿ॳݻ伏૱ᡆߌӗ૱ѝߌ㦟↻⮉Ⲵ∛ݻᮠ㺘⽪˄mg/kg ᡆ ppm˅Ǆ ߌ㦟ᴰབྷ↻⮉䲀䟿˄MRL˅ᱟ䙊䗷ሩߌ㦟䘋㹼ᒯ⌋Ⲵ∂⨶ᆖ䈅傼઼⴨ሩҾᴍ䵢 ᓧӗ⭏ڕ䇱нՊሩӪ㊫؍䟿Ⲵ㟣伏仾䲙䇴ՠˈ⺞ᇊⲴਟ᧕ਇⲴߌ㦟↻⮉≤ᒣˈԕ н㢟ᖡ૽Ǆߌ㦟ᴰབྷ↻⮉䲀䟿ᇎ䱵кᱟߌ㦟ਸ⨶֯⭘઼ߌӗ૱䍨᱃ᡰ䴰㾱䚥ӾⲴ ḷ߶ǄᴹӋഭᇦ׍ᦞ⌅ᖻ⌅㿴Ⲵ㾱≲ˈՊࡦᇊᵜഭߌӗ૱ᴰབྷ↻⮉䲀䟿ḷ߶ˈ㘼 Codex MRL ֌Ѫഭ䱵伏૱⌅ިငઈՊ伏૱ḷ߶Ⲵа䜘࠶ˈ⭡ Codex ߌ㦟↻⮉င ઈՊ׍ᦞ FAO/WHO ߌ㦟↻⮉㚄ᑝՊ䇞˄JMPR˅Ⲵᔪ䇞ࡦᇊⲴǄJMPR ᱟ⭡㚄 ਸഭ㋞ߌ㓴㓷઼ц⭼ছ⭏㓴㓷уᇦޡ਼㓴ᡀⲴ、ᆖ亮䰞㓴㓷ˈJMPR 䙊䗷ሩߌ㦟 ˅ᆖᮠᦞ઼↻⮉ᮠᦞ䘋㹼䇴ԧˈ׍ᦞ䇴ԧ㔃䇪ˈੁഭ䱵伏૱⌅ިငઈՊ˄Codex⨶∂ ᔪ䇞ᴰབྷ↻⮉䲀䟿ḷ߶˄MRL˅Ǆ ᙫѻˈ䙊䗷↻⮉䈅傼ˈ࠶᷀Ự⍻Ҷ MON 87427 н਼⦹㊣ᵲӔ⿽ᡰ᭦㧧㊭㋂ Ⲵ㥹⭈㟖↻⮉≤ᒣǄ൘㊭㋂ѝỰ⍻ࡠⲴᴰ儈↻⮉䟿䘌վҾ Codex ൘ᑨ㿴⦹㊣к 䇮・Ⲵᴰབྷ↻⮉䲀䟿≤ᒣǄสҾ GA21 ᔪ・Ⲵส⹰↻⮉ᮠᦞ㺘᰾ˈ⴨ሩҾ⦹㊣㊭㋂ˈ⦹㊣⋩ѝⲴ㥹⭈㟖↻⮉≤ᒣᶱབྷ䱽վˈ⦹㊣㊹↻⮉≤ᒣ⴨∄㊭㋂〽ᴹ⎃㕙Ǆ ᰐ䇪ᱟ⦹㊣㊹䘈ᱟ⦹㊣⋩ˈ㥹⭈㟖↻⮉䟿䜭䘌վҾ Codex ൘⦹㊣㊭㋂к䇮ᇊⲴ ٬Ǆ MRL

XXXVII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߌъ䜘ޣҾMON 87427ᆹޘ䇴ԧ⭣䈧Ⲵഎ༽࠭ ˄2013ᒤ5ᴸ21ᰕ˅

XXXVIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ਮީӄߒѐ䜞᢯གྷؗ࠳Ⲻ䈪᱄ޢᆕኧ䜳

⍻Ựޘ䰞仈 1˖ᵚᨀ׋ߌъ䜘ငᢈỰ⍻ᵪᶴࠪާⲴ䖜สഐ⦹㊣Ⲵ⧟ຳ઼伏⭘ᆹ ᣕ੺Ǆ 䈤᰾˖᤹➗ߌъ䜘、ᢰਁኅѝᗳޣҾᣇ䲔㥹ࡲ⦹㊣ MON 87427 Ⲵ⧟ຳᆹޘ઼ 伏⭘ᆹޘỰ⍻ԫ࣑л䗮䙊⸕ˈ⭡ߌߌъ䜘䖜สഐ⭏⢙伏⭘ᆹޘⴁⶓỰ傼⍻䈅ѝᗳ ˄ཙ⍕˅᳘ཙ⍕ᐲ⯮⯵亴䱢᧗ࡦѝᗳ઼ߌߌъ䜘䖜สഐἽ⢙⧟ຳᆹޘⴁⶓỰ傼⍻ 䈅ѝᗳ˄䮯᱕˅᳘ਹ᷇ⴱߌъ、ᆖ䲒࠶࡛ᆼᡀҶ MON 87427 ⦹㊣Ⲵ伏⭘ᆹޘ ઼⧟ຳᆹޘỰ⍻ԫ࣑ˈ⴨ޣỰ⍻ᣕ੺࠶࡛㿱⭣䈧Җл޼ᢰᵟᣕ੺ 12 ઼ᢰᵟᣕ੺ 13Ǆ 伏⭘ᆹޘỰ⍻㔃᷌㺘᰾˖䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ MON 87427 ѝᣇ㩕 ޫഐᆀἽ䞨ਜ਼䟿Ѫ 20.3 mg/gˈ䶎䖜สഐᑨ㿴⦹㊣ EXP262 ѝἽ䞨ਜ਼䟿Ѫ 20.2 mg/gˈ є㘵⋑ᴹᱮ㪇ᐞᔲǄ ሶ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ MON 87427 ઼䶎䖜สഐӢᵜ⦹㊣ EXP262 侢ᯉ˄∄ֻѪ 50% ǃ25% ǃ12.5%˅ல侢བྷ啐 90 ཙˈல侢ᵏ䰤ࣘ⢙⍫ޕ᧪࠶࡛ ࣘ㠚ྲˈ㻛∋ᴹݹ⌭ˈ啫ǃ⵬ǃਓ㞄ᰐᔲᑨ࠶⋼⢙Ǆ㔏䇑㔃᷌㺘᰾䜘࠶ᑨ㿴ሩ➗ ৺䖜สഐਇ䈅⢙㓴ࣘ⢙䘨伏䟿оᑨ㿴ส⹰侢ᯉሩ➗㓴∄䖳ˈᴹ㔏䇑ᆖ᜿ѹ ˄P<0.05˅ˈն൷ᰐࡲ䟿৽ᓄޣ㌫ˈᵚ㿱ަ⭏⢙ᆖ᜿ѹǄަ։਴㓴ࣘ⢙փ䟽ǃ䘋伏䟿ǃᙫ໎䟽ǃᙫ䘋伏䟿઼ᙫ伏⢙࡙⭘⦷оᑨ㿴ส⹰侢ᯉሩ➗㓴∄䖳ˈᐞᔲ൷ᰐ 㔏䇑ᆖ᜿ѹ˄P>0.05˅Ǆ ᇎ傼ѝᵏ઼ᇎ傼ᵛᵏ਴亩㹰⏢ᆖᤷḷ˄㓒㓶㜎䇑ᮠǃ㹰㓒㳻ⲭǃ㹰ሿᶯǃⲭ 㓶㜎䇑ᮠ৺࠶㊫˅ǃ㹰⭏ॆᤷḷ˄㹰␵щ≘䞨≘ส䖜〫䞦ǃཙ䰘ߜ≘䞨≘ส䖜〫䞦ǃቯ㍐≞ǃ㛶പ䞷ǃ⭈⋩й䞟ǃ㹰㌆ǃң䞨㝡≒䞦ǃᙫ㳻ⲭǃⲭ㳻ⲭǃ㚼䞀ǃ ᙗ⼧䞨䞦˅൷൘ᵜỰ⍻অս↓ᑨ٬㤳ത޵Ǆࣘ⢙䀓ࢆᰦˈབྷփ㿲ሏᵚਁ⧠ᔲᑨǄ⻡䳴ᙗࣘ⢙Ӣѝ㓴㛮/փ∄վҾሩ➗㓴ˈӢ儈㓴㛍/փ∄儈Ҿሩ➗㓴ˈ䖜վ㓴㛍/փ∄ 儈Ҿሩ➗㓴ˈ䖜վ㓴ᗳ/փ∄儈ҾӢվ㓴ˈ䖜ѝ㓴㛮к㞪/փ∄儈ҾӢѝ㓴ˈᐞᔲ ᴹ㔏䇑ᆖ᜿ѹ˄P<0.05˅Ǆ䳼ᙗࣘ⢙Ӣ儈㓴㜨㞪/փ∄ǃ㛮/փ∄儈Ҿሩ➗㓴ˈ䖜 ѝ㓴㛍/փ儈Ҿሩ➗㓴ˈ䖜儈㓴㜨㞪/փ∄ǃ㛮/փ∄儈Ҿሩ➗㓴ˈ䖜ѝ㓴㛍/փ儈Ҿ Ӣѝ㓴ˈᐞᔲᴹ㔏䇑ᆖ᜿ѹ˄P<0.05˅Ǆնк䘠ᐞᔲᰐࡲ䟿৽ᓄޣ㌫ˈᵚ㿱ަ⭏ ٬оሩ➗㓴∄䖳ˈᐞᔲ൷ᰐ㔏䇑ᆖ∄ᆖ᜿ѹǄަ։਴㓴ࣘ⢙㜿ಘ䟽䟿઼㜿/փ⢙ ᜿ѹ˄P<0.05˅Ǆ⯵⨶㓴㓷ᆖỰḕᵚਁ⧠䈕䖜สഐṧ૱ᕅ䎧ᇎ傼ࣘ⢙ިර⯵⨶㓴㓷ᆖ᭩ਈ઼⢩ᔲᙗᦏՔǄ 㔬кˈ90 ཙབྷ啐ޘ伏૱ல侢䈅傼㺘᰾ˈ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣侢ᯉ˄∄ֻѪ 50% ǃ25% ǃޕ᧪MON 87427 ઼䶎䖜สഐӢᵜ⦹㊣ EXP262 ࠶࡛ 12.5%˅ல侢བྷ啐 90 ཙˈᵚ㿱ަሩࣘ⢙ӗ⭏н㢟ᖡ૽Ǆ ⧟ຳᆹޘỰ⍻㔃᷌㺘᰾˖ਇߌъ䜘Ⲵငᢈˈߌъ䜘䖜สഐἽ⢙⧟ຳᆹޘⴁⶓ Ự傼⍻䈅ѝᗳ˄䮯᱕˅᳘ਹ᷇ⴱߌъ、ᆖ䲒Ҿ 2014 ᒤᆼᡀҶሩ MON 87427 ᣇ䲔㥹ࡲ⦹㊣Ⲵ⧟ຳᆹޘᙗ䇴ՠǄ

XXXIX ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䲔㥹ࡲ㙀ਇᙗ䢤ᇊ㔃᷌㺘᰾ˈо䶎䖜สഐ⦹㊣ሩ➗૱⿽⴨∄ˈ௧ᯭ᧘㦀ࡲ䟿 ৺ 2 ؽ᧘㦀ࡲ䟿ߌ䗮䲔㥹ࡲˈሩ䖜สഐ⦹㊣ MON87427 ἽṚⲴ⭏䮯ਁ㛢ᰐ᰾ᱮ ᖡ૽ˈ㺘᰾䖜สഐᣇ䲔㥹ࡲ⦹㊣ MON87427 ሩߌ䗮䲔㥹ࡲާᴹ㢟ྭⲴ㙀ਇᙗǄ ⭏ᆈㄎҹ㜭࣋Ự⍻㔃᷌㺘᰾ˈ൘㦂ൠ䈅傼ᶑԦлˈ䖜สഐ⦹㊣ MON87427 Ⲵ⭏ᆈㄎҹ㜭࣋ᱮ㪇ᕡҾᵲ㥹ˈфоަሩᓄⲴ䶎䖜สഐ⦹㊣ᰐᱮ㪇ᐞᔲ˗൘ṭษ ൠ䈅傼ᶑԦлˈ䖜สഐ⦹㊣ MON87427 ൘Ṛ儈ǃ㾶ⴆᓖǃӗ䟿ǃਾԓ⿽ᆀਁ㣭 ⦷ㅹᯩ䶒ˈоሩᓄⲴ䶎䖜สഐ⦹㊣ᰐᱮ㪇ᐞᔲǄ ሩ⭏⢙ཊṧᙗᖡ૽Ự⍻㔃᷌㺘᰾ˈо䶎䖜สഐ⦹㊣⴨∄ˈ䖜สഐ⦹㊣ MON87427 ሩ⭠䰤ѫ㾱㢲㛒ࣘ⢙ᮠ䟿઼㗔㩭㔃ᶴǃѫ㾱匎㗵ⴞᇣ㲛ԕ৺ѫ㾱⯵ᇣ ᰐᱮ㪇ᖡ૽Ǆ

㔬ਸ䇴ԧᣕ੺Ǆޘ䰞仈 2˖ᵚᨀ׋䖜สഐ⦹㊣Ⲵ⧟ຳ઼伏⭘ᆹ 䈤᰾˖ᣇ䲔㥹ࡲ䖜สഐ⦹㊣ MON 87427 Ⲵ⧟ຳᆹޘ઼伏⭘ᆹޘ㔬ਸ䇴ԧᣕ੺ 䱴Ԧ䍴ᯉāѝ˄ㅜ 174 亥ǉ䖜 cp4 epspsޣ䜘࠶Ā⴨ޝᐢ㺕ݵ㠣ᵜ⭣䈧Җ↓᮷ㅜ สഐ㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣ MON 87427 Ⲵ⧟ຳ઼伏⭘ᆹޘᙗ㔬ਸ䇴ԧᣕ ੺Ǌ˅Ǆ

XL ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ഭ޵ཆᆹޘ䇴ԧᢰᵟᣕ੺ⴞᖅ ˄ᢰᵟᣕ੺৏᮷৺㘫䈁Ԧ䈧㿱ᵜ⭣䈧Җѝл޼޵ᇩ˅

⭣䈧Җѝ޼˄ᢰᵟᣕ੺1~8˅

㕆ਧ ᢰᵟᣕ੺仈ⴞ 亥⸱

ᣕ੺ 1˖ MON 87427 ⦹㊣Ⲵ࠶ᆀ⢩ᖱ࠶᷀˄ᆏኡ䜭ޜਨ⹄ウᣕ੺˖ <1> MSL0021822˅

ᣕ੺ 2˖ 2008 ᒤ㖾ഭ⭠䰤䈅傼 MON 87427 ⦹㊣㓴㓷ѝⲴ CP4 EPSPS <76> 㳻ⲭ㺘䗮≤ᒣ䇴ՠ˄ᆏኡ䜭ޜਨ⹄ウᣕ੺˖MSL0022370˅

ᣕ੺ 3˖ MON 87427 ⦹㊣ཊцԓਦ⡷઼⿽ᆀṧ૱ѝ CP4 EPSPS 㳻ⲭ < 110> Ⲵ Western ঠ䘩࠶᷀˄ᆏኡ䜭ޜਨ⹄ウᣕ੺˖MSL0022026˅

ᣕ੺ 4a˖2008 ᒤ㖾ഭ⭠䰤䈅傼ѝ MON 87427 Ⲵ㺘ර઼⭏ᘱӂ֌䇴ԧ < 135 > ˄ᆏኡ䜭ޜਨ⹄ウᣕ੺˖MSL0022501˅

ᣕ੺ 4b: 2008-2009 ᒤ㖾ഭབྷ⭠䖜สഐ⦹㊣ MON 87427 㠚⭏㤇▌࣋䇴 < 297 > ԧ˄ᆏኡ䜭ޜਨ⹄ウᣕ੺˖MSL000022540˅

ᣕ੺ 5a˖MON 87427 ㊭㋂ᶕⓀ CP4 EPSPS 㳻ⲭⲴ⢩ᙗ䢤ᇊ৺Ἵ⢙ᶕ < 334 > Ⓚ઼བྷ㛐ᵶ㧼ᶕⓀⲴ CP4 EPSPS 㳻ⲭ⨶ॆᙗ䍘઼࣏㜭⢩ᙗ ∄䖳˄ᆏኡ䜭ޜਨ⹄ウᣕ੺˖MSL0023119˅

ᣕ੺ 5b˖MON 87427 ⦹㊣㊭㋂ᨀਆⲴ CP4 EPSPS 㳻ⲭ઼བྷ㛐ᵶ㧼ӗ < 402 > ⭏Ⲵ CP4 EPSPS 㳻ⲭ⢩ᙗ࠶᷀˄ᆏኡ䜭ޜਨ⹄ウᣕ੺˖ SCR-2016-0287˅

ᣕ੺ 6˖ ࡙⭘ᴤᯠⲴ AD_2016ǃTOX_2016ǃPRT_2016 ᮠᦞᓃሩ < 427 > ˖CP4 EPSPS Ⲵ⭏⢙ؑ᚟ᆖ䇴ԧᴤᯠ˄ᆏኡ䜭ᢰᵟᣕ੺ RAR-2016-0208˅

ᣕ੺ 7˖ ࡙⭘ᴤᯠⲴ AD_2016ǃTOX_2016ǃPRT_2016ǃEST_2016ǃ < 441 > NT_2016 ઼ NR_2016 ᮠᦞᓃሩ MON 87427 Ⲵ⭏⢙ؑ᚟ᆖ䇴 ԧᴤᯠ˄ᆏኡ䜭ޜਨ⹄ウᣕ੺˖RAR-2016-0445˅

ᣕ੺ 8˖ CP4 EPSPS 㳻ⲭ൘⁑ᤏ㛳⏢ѝⲴ⎸ॆ⦷䇴ԧ˄ᆏኡ䜭ޜਨ⹄ < 479 > ウᣕ੺˖MSL0017566˅

XLI ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⭣䈧Җл޼˄ᢰᵟᣕ੺9~18˅

㕆ਧ ᢰᵟᣕ੺仈ⴞ 亥⸱

ᣕ੺ 9˖ ࣐✝༴⨶ሩ CP4 EPSPS ࣏㜭⍫ᙗⲴᖡ૽˄ᆏኡ䜭ޜਨ⹄ウᣕ < 530 > ੺˖MSL0023307˅

ᣕ੺ 10˖CP4 EPSPS 㳻ⲭⲴሿ啐ᙕᙗਓᴽ∂ᙗ䈅傼˄ᆏኡ䜭ޜਨ⹄ウᣕ < 560 > ੺˖MSL0013077˅

ᣕ੺ 11˖2008 ᒤ㖾ഭ⭠䰤䈅傼ѝ MON 87427 ⦹㊣〨⿶઼㊭㋂Ⲵᡀ࠶࠶ < 592 > ᷀˄ᆏኡ䜭ޜਨ⹄ウᣕ੺˖MSL0022340˅

ᣕ੺ 12˖MON 87427 ⦹㊣伏⭘ᆹޘỰ⍻ᣕ੺˄ߌъ䜘䖜สഐ⭏⢙伏⭘ < 799 > ᆹޘⴁⶓỰ傼⍻䈅ѝᗳ˄ཙ⍕˅ˈ2014 ᒤ 10 ᴸ˅

ᣕ੺ 13˖MON 87427 ⦹㊣⧟ຳᆹޘỰ⍻ᣕ੺˄ߌъ䜘䖜สഐἽ⢙⧟ຳ <823 > ᆹޘⴁⶓỰ傼⍻䈅ѝᗳ˄䮯᱕˅ˈ2014 ᒤ 10 ᴸ˅

ᣕ੺ 14˖བྷ㛐ᵶ㧼㺘䗮㓟ॆⲴ CP4 EPSPS 㳻ⲭሩሿ啐Ⲵᙕᙗਓᴽ∂ᙗ <858> ⹄ウ˄ᆏኡ䜭ޜਨ⹄ウᣕ੺˖MSL0026454˅

ᣕ੺ 15˖བྷ㛐ᵶ㧼ᶕⓀ CP4 EPSPS 㳻ⲭ㓿ਓ♼㛳 CD-1 ሿ啐Ⲵᙕᙗ∂ᙗ <918 > ⹄ウ-5000mg/kg փ䟽˄ᆏኡ䜭⹄ウᣕ੺˖MSL0027235˅

ᣕ੺ 16a˖ᯭ⭘㥹⭈㟖ࡦࡲਾ MON 87427 ⦹㊣㋇࣐ᐕߌӗ૱ѝ㥹⭈㟖↻ < 987 > ⮉䟿࠶᷀˄ᆏኡ䜭ޜਨ⹄ウᣕ੺˖MSL0021925˅

ᣕ੺16b˖䎺亦௧ᯭ㥹⭈㟖䲔㥹ࡲਾˈMON 87427 ⦹㊣࣐ᐕ㓴࠶ѝ㥹⭈ < 998 > 㟖↻⮉䟿৺⎃㕙㌫ᮠ⍻ᇊⲴᙫ㔃ᣕ੺˄ᆏኡ䜭ޜਨ⹄ウᣕ੺˖ MSL0028092˅

ᣕ੺ 17˖MON 87427 ⦹㊣㊭㋂⭘֌侢ᯉᡀ࠶ሩᯁ⛩৹ቮ几Ⲵᖡ૽䇴ՠ < 1008 > ˄ᆏኡ䜭ޜਨ⹄ウᣕ੺˖SE-2010-0626˅

ᣕ੺ 18˖侢லਜ਼ MON 87427ǃሩ➗ᡆ৲➗⦹㊣㊭㋂侢ᯉⲴ㚹呑䓛փᙗ <1045 > 㜭઼ᇠᵰਾ਴亩৲ᮠ∄䖳˄ᆏኡ䜭ޜਨ⹄ウᣕ੺˖ MSL0022485˅

XLII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᇶ୶ъ䍴ᯉ˄CBI˅༠᰾؍

ᇶ୶ъ䍴ᯉ˄CBI˅Ǆ؍ਨᔰਁᒦᤕᴹⲴޜᵜ⭣䈧Җवਜ਼Ҷ䇨ཊ኎Ҿᆏኡ䜭 ᐘབྷⲴӪ࣋⢙࣋ˈ㓿䗷䮯ᰦ䰤Ⲵ⹄ウ㧧ޕਨᣅޜᇶ୶ъ䍴ᯉᱟ⭡ᆏኡ䜭؍䘉Ӌ ᗇⲴˈवᤜ⹄ウ䐟㓯Ⲵࡦᇊǃ਴⿽㺘䗮䈳᧗ݳԦⲴ DNA ᒿࡇǃ䚇Ր᫽֌ㆆ⮕ ઼਴⿽ᆹޘ䇴ԧⲴᮠᦞㅹ䍴ᯉǄᆏኡ䜭ޜਨⴞࡽਚੁц⭼਴ഭߌъ䖜สഐ⭏⢙ 䇴ԧ઼ᇑᢩǄаᰖᆏኡ䜭ޘᇶ䍴ᯉˈ⭘Ҿ⌅㿴ᆹ؍㹼᭯ѫ㇑䜘䰘ᨀӔ䘉Ӌ୶ъ ޜਨⲴㄎҹሩ᡻㧧ᗇ䘉Ӌ䍴ᯉˈㄎҹሩ᡻ሶՊԕᖸቁӪ࣋⢙࣋ᣅޕ൘ᖸ⸝Ⲵᰦ 䰤޵ᔰਁࠪ㊫լ MON 87427 Ⲵ⴨ᓄӗ૱ˈ䘉ሩᆏኡ䜭ޜਨᶕ䈤ᱟнޜᒣⲴǄ ᆏኡ䜭ޜਨ൘ѝഭഭ޵ཆ䶒Ѥ⵰䇨ཊᢰᵟᇎ࣋ᖸᕪⲴㄎҹሩ᡻Ǆഐ↔ˈᆏኡ䜭㿴ᇊޣ䇴ԧ㇑⨶࣎⌅ǊㅜйㄐㅜҼॱгᶑⲴᴹޘਨ׍ᦞǉߌъ䖜สഐ⭏⢙ᆹޜ ᇶǄ؍ᇶ䍴ᯉ䘋㹼؍ԕ৺ᵜ⭣䈧ҖⲴປ߉䈤᰾ˈ㾱≲ሩᵜ⭣䈧Җḷᴹ CBI Ⲵ୶ъ

XLIII ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 аǃ⭣䈧㺘 ঠഃۜġ

૊ྜᅧݮ࿙ಓ๞̝௦ᄃೠಃைζ

MON 87427 ࠩࣇဈᆴަٝၐॸԅ̝௦ᄃೠ ਼ံޔ੠ы ᅧ cp4 epsps ݮ࿙ઐ҂Єંົ ࣿၗ ਴ౖ՛٤ഓંົ फι Վ๞ Ƙᄑ๞ ฑಓ๞ ᅧݮ࿙ಓ๞੠ы MON 87427

ᄯำ੠ ံ਼ ༰੠ Zea mays L. ೓඘ ᅧ फ༰ ۣ·ࢳc ံ೨೩೫c ଶᄵďଶຂ Đד ಓ๞ LH198 × HiII ̝௦ԉމ 1 ԙส ံ਼ᄵ ੠ы ݮ Agrobacterium tumefaciens ٢඘ಓ๞ ੠ы cp4 epsps ંԅ sp.CP4 ࿙ ݮ࿙ ޔᤐफ҂Єإಓ๞༰ٟટ ζӒ CP4 EPSPS ӱ͂ēರᅧݮ࿙ᄑ๞ઐ೓Є ಓ ٢඘ಓ๞ ԅ ੠ыં ๞ ݮ࿙ ಓ๞༰ٟટ ٢඘ಓ๞ Escherichia coli ၻ඘ PV-ZMAP1043 ؠ ํ ٢඘ಓ๞ ํ ݮ࿙ޚγ ํ ٢඘ಓ๞ ํ ݮ࿙فࣣ ͱ ݮ࿙Ѐᆴफ໸ 2 ܤࢉࠚӽᅧئᅧݮ࿙ֺ֥ ૊

1 މᅧݮ࿙ಓ๞̝௦ԉ

1 މᅧݮ࿙ಓ๞Сଶ̝௦ԉ

Ωۜރᄯ޷ೋི ᅧݮ࿙ಓ๞੠ы ੦ڟெࣣ ೋིԅನ޷cԙԤۤ Ωۜރᅧݮ࿙ಓ๞੠ы ೋ ࡂೄׂܬ ଛᅹำۜ ི ெࣣ ੦ڟؠ ଛᅹನ޷cԙԤۤ ࣣ Ωۜރᅧݮ࿙ಓ๞੠ы ಓС໿ೋི ଛᅹำۜ ெࣣ ੦ڟଛᅹನ޷cԙԤۤ

1 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ڳથಃைರဈֳถďಘcᆑᄭ௕cᄐຊೇĐ ௦ થಃைರဈભຫ 5 Ӧส੠ы ਴ౖ՛ၙՊပຫ٤ഓ ֥ఆӝζ ಃ Ӧส໿ᄩ ࡂෳ୲ྜӦส ை Ӧสԙᄖ ဏΩ 100028 Ӧ ಃைఆༀ੠ Ԩܥ ส ؠ Ҏყ Ԩᆐဏັ ࣣ ॓ຂఆༀ੠ Ԩܥ Ҏყ Ԩᆐဏັ Ӧส੠ы ਴ౖ՛٤ഓ ֥ఆӝζ ॓ຂఆༀ੠ Ԩܥ ཙ Ҏყ Ԩᆐဏັ ᅖྑ෻юఆ ᄥ ༀ੠ ໿ι ѻಓભၥ

Ӧ ༰ॅ ᅥྜޏ೬ᄏ๠ ۥԙႛҶಹۥನۥ ส ᄵݮ࿙ٝёٝᆴ ϵူ෻юఆ ٝדؠ ༀ੠ ભ঍ ༰ॅ ᄏы Ӧส ၽ·ົંᄯԅ

ࣣ

2 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 4 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 5 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 6 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 7 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 8 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ҽǃ亩ⴞ޵ᇩ᪈㾱 5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦˄EPSPS˅ᒯ⌋ᆈ൘ҾἽ⢙઼ᗞ⭏⢙փ޵ˈ 䭞䞦ˈ㘼㧭㥹䞨䙄ᖴ䍏䍓൘Ἵ⢙઼ᗞ⭏⢙փ޵ᨀ׋㣣俉ޣᱟ㧭㥹䞨䙄ᖴѝⲴањ ⧟ˈ⭘Ҿ㣣俉᯿≘ส䞨˄㤟щ≘䞨ǃ䞚≘䞨ǃ㢢≘䞨˅઼䇨ཊ⅑㓗⢙˄ྲഋ≒ਦ 䞨ǃ⌋䞼ǃ㔤⭏㍐ Kǃ㣡䶂㍐ǃ㊫哴䞞ǃᵘ䍘㍐ㅹ˅Ⲵ⭏⢙ਸᡀ˄Herrmann and Weaver 1999; Maeda and Dudareva 2012˅Ǆ䈕䙊䐟൘Ἵ⢙փ޵䶎ᑨ䟽㾱ˈἽ⢙փ㠣 ቁ 35%Ⲵᒢ⢙䍘ᶕⓀҾ㧭㥹䞨䙄ᖴⲴӗ⢙˄Franz et al. 1997˅Ǆԕ㥹⭈㟖Ѫᴹ᭸ ᡀ࠶Ⲵ䲔㥹ࡲߌ䗮®ᱟа㊫䶎䘹ᤙᙗ䲔㥹ࡲˈ㥹⭈㟖ਟԕᣁࡦἽ⢙޵ⓀⲴ EPSPS 䞦⍫ᙗˈӾ㘼䱫ᯝἽ⢙⭏䮯ᡰᗵ䴰Ⲵ㣣俉᯿≘ส䞨Ⲵ⭏⢙ਸᡀˈሬ㠤Ἵ⢙↫ӑˈ ᱟ䶎ᑨᴹ᭸Ⲵ䲔㥹ࡲˈ⭘Ҿ䱢⋫аᒤ⭏ᡆཊᒤ⭏Ⲵঅᆀਦᵲ㥹઼䱄ਦ㊫ᵲ㥹Ǆ儈 ㅹ⭏⢙ྲପңࣘ⢙փ޵нᆈ൘㧭㥹䞨䙄ᖴˈਚ㜭Ӿ伏⢙ѝ㧧ਆ㣣俉᯿≘ส䞨˗⭡ Ҿ㠚䓛н㜭ਸᡀ EPSPS 䞦ˈପңࣘ⢙ሩ㥹⭈㟖н᭿ᝏǄ ᆏኡ䜭ޜਨᔰਁⲴ MON 87427 ⦹㊣˄OECD ୟа䇶࡛ਧѪ MON-87427-7˅ ਟԕ䘹ᤙᙗൠ൘аӋ㓴㓷ѝ㺘䗮ߌᵶ㧼 CP4 㧼ṚⲴ 5-✟䞷щ䞞䞠-3-⼧䞨䖜〫䞦 ˄CP4 EPSPS˅ǄMON 87427 ࡙⭘Ҷ⢩ᇊⲴ੟ࣘᆀ-޵ਜ਼ᆀ㓴ਸ˄e35S-hsp70˅ˈ ণ໎ᕪර 35S ੟ࣘᆀ઼⦹㊣✝◰㳻ⲭ Hsp70 㕆⸱ᒿࡇⲴㅜањ޵ਜ਼ᆀǄ䈕੟ࣘ ᆀ-޵ਜ਼ᆀ㓴ਸਟԕ⢩ᔲᙗൠ൘㩕ޫ⭏䮯ಘᇈ˄ਦ⡷ǃ〨⿶઼ṩ˅઼䳼ᙗ⭏⇆ಘ ֯ˈᇈ˄ਟԕ䘋а↕ਁ㛢ᡀ⿽ᆀᡆ㊭㋂઼㣡эⲴ㓴㓷˅ѝ㺘䗮 CP4 EPSPS 㳻ⲭ 䘉Ӌ㓴㓷઼ಘᇈਟԕᣇ㥹⭈㟖Ǆն CP4 EPSPS 㳻ⲭ൘㣡㊹ሿᆒᆀ˄ਟԕ䘋а↕ 㛢ᡀ㣡㊹㋂˅઼∑㔂ቲ㓶㜎˄Ѫ㣡㊹㋂ਁ㛢ᨀ׋㩕ޫ˅ѝ㺘䗮䟿ᶱվˈࠐѾᰐਁ ⌅Ự⍻ࡠǄഐ↔ˈMON 87427 Ⲵ䳴ᙗಘᇈнᣇ㥹⭈㟖Ǆ൘ MON 87427 ⦹㊣⢩ᇊ ⭏䮯䱦⇥˄V8~V13 ⭏䮯䱦⇥˅௧ᯭ㥹⭈㟖䲔㥹ࡲˈӗ⭏Ⲵ㣡㊹нާᴹ⍫࣋ˈഐ ↔ˈ൘⦹㊣ᵲӔ⿽ࡦ⿽䗷〻ѝˈਟԕ⭘ਜ਼ᴹ MON 87427 䖜ॆփⲴ⦹㊣㠚Ӕ㌫֌ ᵜˈُࣙҾ㥹⭈㟖䲔㥹ࡲⲴ௧ᯭӗ⭏䳴ᙗн㛢ˈݽ৫Ӫᐕᣭ䳴Ⲵ↕僔ˈབྷབྷ㢲⇽ 㓖Ҷࡦ⿽ᡀᵜǄ MON 87427 ᱟ䙊䗷ߌᵶ㧼ӻሬ䖜ॆˈሶ PV-ZMAP1043 䍘㋂ѝⲴ T-DNA ሬ ޕࡠ LH198 × HiII ਇփ૱㌫ѝ㧧ᗇⲴǄ䈕 T-DNA वਜ਼ᴹањ cp4 epsps สഐ㺘䗮 ⴂˈवᤜ e35S ੟ࣘᆀǃhsp70 Ⲵ޵ਜ਼ᆀǃCTP2 ਦ㔯փ䖜〫㛭ᒿࡇǃcp4 epsps 㕆 ⸱ᒿࡇ઼ nos 㓸→ᆀǄSouthern ᵲӔ࠶᷀㺘᰾ˈᶕ㠚Ҿ䖜ॆ䍘㋂ PV-ZMAP1043 ѝⲴ T-DNA ᒿࡇӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ൘⦹㊣สഐ㓴Ⲵঅњสഐᓗк˗ᒦфˈ 䲔Ҷ T-DNA ཆˈPV-ZMAP1043 䍘㋂Ⲵަᆳ䈳᧗ݳԦ˄वᤜ僘ᷦᒿࡇ˅൷⋑ᴹᨂ ޕࡠ MON 87427 Ⲵสഐ㓴ᒿࡇѝǄPCR ઼ DNA ᒿࡇ࠶᷀䇱ᇎҶ T-DNA ᨂޕⲴ 㘬Ⲵสഐ㓴ᒿࡇǄחᒿࡇޕ㔃ᶴᱟᆼᮤⲴˈަ㔃ᶴо䖜ॆ䍘㋂а㠤˗ᒦ⺞ᇊҶᨂ ↔ཆˈ䘈䙊䗷 Southern ᵲӔ࠶᷀䇱ᇎҶ MON 87427 ѝⲴཆⓀᨂޕ൘ 5 њ㛢⿽ц ؍Ⲵ DNA ᒿࡇ൘ཊцԓ䰤ޕᙗ⣦Ⲵ䚇Ր࠶⿫ᮠᦞҏ䇱ᇎᨂޕᤱっᇊˈሬ؍ԓ䰤 ᤱっᇊǄ 㳻ⲭ䍘⢩ᖱ⢩ᙗ⹄ウ㔃᷌㺘᰾ˈMON 87427 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭоᐢ 㓿൘ѝഭԕ৺ц⭼ަԆഭᇦ㧧ᗇᢩ߶Ⲵཊ⿽ᣇ㥹⭈㟖֌⢙ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭᱟ⴨਼ⲴǄ䘉Ӌӗ૱ѝⲴ CP4 EPSPS 㳻ⲭⲴᆹޘᙗᐢ㓿㻛བྷ䟿Ⲵᆹޘᙗ䇴 ᓧᖡ૽Ⲵڕ⢙Ҿ MON 87427 ሩӪ઼ପңࣘޣԧ⹄ウᡰ䇱ᇎǄᵜ⭣䈧Җᡰᨀ׋Ⲵ 9 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 伏⭘ᆹޘ䇴ԧ䍴ᯉवᤜ˖1˅CP4 EPSPS 㳻ⲭⲴᆹޘᓄ⭘শਢ˗2˅CP4 EPSPS 㳻ⲭⲴ⨶ॆ⢩ᙗ઼࣏㜭࠶᷀˗3˅MON 87427 ⦹㊣ѝ CP4 EPSPS 㳻ⲭ㺘䗮䟿࠶ ᷀˗4˅࡙⭘⭏⢙ؑ᚟ᆖᯩ⌅ሩ CP4 EPSPS 㳻ⲭоᐢ⸕Ⲵ∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃ ᓧᴹн㢟ᖡ૽Ⲵާᴹ⭏⢙⍫ᙗ㳻ⲭ䘋㹼⴨ڕ⢙㠤᭿㳻ⲭᡆަᆳᐢ⸕ሩӪ㊫઼ࣘ լᙗ࠶᷀ˈᵚਁ⧠ CP4 EPSPS ок䘠∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃ㠤᭿㳻ⲭᡆᱟާ ᴹн㢟⭏⢙⍫ᙗ㳻ⲭѻ䰤ᴹԫօ㔃ᶴ⴨լᙗ˗5˅փཆ⎸ॆ࠶᷀㺘᰾ˈCP4 EPSPS 㳻ⲭ൘⁑ᤏ㛳⏢˄SGF˅ᶑԦл㻛䗵䙏⎸ॆ˗6˅✝っᇊᙗ࠶᷀㺘᰾ˈCP4 EPSPS 㳻ⲭ䲿⵰⑙ᓖкॷަ⍫ᙗ䗵䙏їཡ˗7˅ṩᦞᴰᯠᆼᡀⲴሿ啐ᙕᙗਓᴽᇎ傼ˈ CP4 EPSPS 㳻ⲭⲴᰐ᰾ᱮᦏᇣ֌⭘ࡲ䟿˄NOAEL˅㠣ቁѪ 5000 mg/kg bwtǄṩ ᦞѝഭӪ㗔㟣伏㩕ޫᩴޕᮠᦞԕ৺ MON 87427 ㊭㋂Ⲵ CP4 EPSPS 㳻ⲭਜ਼䟿 ˄3.6 μg/g fwt ᡆ 4.2 μg/g dwt˅ˈ䇑㇇ࠪⲴ᳤䵢䲀˄MOE˅儈䗮 6.9 × 106Ǆ ṩᦞ OECD ᤷইˈ࠶᷀Ҷ MON 87427 ⦹㊣Ⲵ㊭㋂઼〨⿶Ⲵ਴⿽㓴ᡀᡀ࠶ˈ वᤜ㩕ޫᡀ࠶ǃᣇ㩕ޫᡀ࠶઼⅑㓗ԓ䉒ᡀ࠶ˈᒦоަᑨ㿴ሩ➗૱⿽䘋㹼∄䖳Ǆ⭠ 䰤䈅傼৺ᡀ࠶࠶᷀ѝ䘈वᤜаӋᐢ㓿୶ъॆᓄ⭘Ⲵ⦹㊣૱⿽ˈ⭘ᶕᔪ・ᑨ㿴⦹㊣ ਴⿽ᡀ࠶Ⲵ 99%ᇩ䇨४䰤Ǆᡀ࠶࠶᷀㔃᷌㺘᰾ˈMON 87427 Ⲵ਴⿽ᡀ࠶о䶎䖜 สഐሩ➗Ⲵᡀ࠶ᇎ䍘ㅹ਼ˈᒦф൷൘ᑨ㿴⦹㊣Ⲵ 99%ᇩ䇨४䰤޵Ǆ ਇߌъ䜘Ⲵငᢈˈ2014 ᒤˈߌъ䜘䖜สഐ⭏⢙伏⭘ᆹޘⴁⶓỰ傼⍻䈅ѝᗳ ˄ཙ⍕˅᳘ཙ⍕ᐲ⯮⯵亴䱢᧗ࡦѝᗳ࡙⭘ MON 87427 ⦹㊣㊭㋂䘋㹼Ҷ伏⭘ᆹޘ Ự⍻Ǆ㔃᷌㺘᰾ˈ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ MON 87427 ѝᣇ㩕ޫഐᆀἽ䞨 ਜ਼䟿Ѫ 20.3 mg/gˈ䶎䖜สഐᑨ㿴⦹㊣ EXP262 ѝἽ䞨ਜ਼䟿Ѫ 20.2 mg/gˈє㘵⋑ ᴹᱮ㪇ᐞᔲǄሶ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ MON 87427 ઼䶎䖜สഐӢᵜ⦹㊣侢ᯉ˄∄ֻѪ 50% ǃ25% ǃ12.5%˅ல侢བྷ啐 90 ཙˈᵚ㿱ަޕ᧪EXP262 ࠶࡛ ሩࣘ⢙ӗ⭏н㢟ᖡ૽Ǆᡰᴹԕк䘉Ӌ࠶᷀㔃᷌൷䇱ᇎ˖MON 87427 ⦹㊣৺ަ㺘 ᓧ⋑ᴹн㢟ᖡ૽Ǆڕ䗮Ⲵ CP4 EPSPS 㳻ⲭሩӪ઼ପңࣘ⢙Ⲵ ↔ཆˈ䘈ሩ MON 87427 Ⲵ㺘රᙗ⣦ǃߌ㢪ᙗ⣦ԕ৺о⧟ຳӂ֌䘋㹼Ҷ䇴ՠˈ ᒦоަᑨ㿴⦹㊣ሩ➗䘋㹼∄䖳Ǆ਼ᰦ൘䈅傼ѝ䘈वᤜҶаӋᐢ㓿୶ъॆ⿽ἽⲴ૱ ⿽ˈ⭘ԕᔪ・ᑨ㿴⦹㊣ᙗ⣦Ⲵ㠚❦ਈᔲ㤳തˈԕׯ൘ࠪ⧠㔏䇑ᆖᱮ㪇ᙗᐞᔲᰦᶕ 䇴ՠ䘉Ӌᱮ㪇ᙗᐞᔲⲴ⭏⢙ᆖ᜿ѹǄ䇴ՠⲴᙗ⣦वᤜ˖⿽ᆀՁⵐ઼㨼ਁ⢩ᙗǃ㣡㊹ᖒᘱᆖ⢩ᖱǃἽṚ㺘රᙗ⣦઼о⧟ຳӂ֌ㅹǄ㔃᷌㺘᰾ˈMON 87427 Ⲵ⿽ᆀ Ձⵐ઼㨼ਁ⢩ᙗо䶎䖜สഐሩ➗⋑ᴹᐞ࡛ˈоᑨ㿴⦹㊣аṧˈMON 87427 ⋑ᴹ ⺜ᇎ⿽ᆀˈ㘼⺜ᇎ⿽ᆀᱟᵲ㥹ᡰާ༷Ⲵިර⢩ᖱǄMON 87427 ઼䶎䖜สഐሩ➗ ൘㣡㊹㋂ⴤᖴᯩ䶒⋑ᴹ㔏䇑ᆖᱮ㪇ᙗᐞᔲ˄=0.05˅ˈMON 87427 Ⲵ㣡㊹⍫࣋〽儈Ҿሩ➗˄99.7% vs. 98.9%˅ˈն MON 87427 Ⲵ㣡㊹⍫࣋о䶎䖜สഐሩ➗Ⲵᐞ࡛ ሿҾ 1%ˈਟԕ䇔Ѫ䘉⿽ሿҾ 1%Ⲵᐞ࡛нާᴹ⭏⢙ᆖ᜿ѹǄ 㺘රᙗ⣦ǃߌ㢪ᙗ⣦઼о⧟ຳӂ֌Ⲵ⭠䰤䈅傼㔃᷌ҏ䘋а↕䇱ᇎˈо䶎䖜ส Ǆ⭠䰤ੁٮഐሩ➗⴨∄ˈMON 87427 нՊ໎࣐֌⢙ᵲ㥹ॆᡆ䖜ਈѪᴹᇣ⭏⢙Ⲵ 䈅傼䇴ՠⲴᙗ⣦䲔Ҷवᤜ 14 њ⭏䮯ਁ㛢ᙗ⣦ཆˈ䘈वᤜἽṚሩ䶎⭏⢙㛱䘛৽ᓄ ԕ৺ἽṚо⯵ᇣ઼ἽṚо㢲㛒ࣘ⢙Ⲵӂ֌ޣ㌫ǄMON 87427 ઼䶎䖜สഐሩ➗ѻ 䰤ᆈ൘Ⲵୟаާᴹ㔏䇑ᆖᱮ㪇ᐞᔲⲴᮠᦞሩ∄ᱟཊ䈅傼⛩㔬ਸᮠᦞ࠶᷀ѝⲴᒬ 㤇⭏⍫࣋ǄMON 87427 Ⲵᒬ㤇⍫࣋վҾሩ➗˄2.4 㓗ሩ 2.7 㓗ˈ㓗࡛ࡂ࠶൘ 1-9 ѻ䰤˅Ǆ⭡Ҿ䘉⿽ᐞ࡛ᮠ٬ᖸሿˈᒦфᰐ䇪ᱟ MON 87427 䘈ᱟᑨ㿴ሩ➗Ⲵᒬ㤇⍫ 10 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ࣋ᮠ٬൷༴൘ᑨ㿴⦹㊣㠚❦ਈᔲⲴ㤳ത޵ˈഐ↔ਟԕ䇔Ѫ䘉⿽㔏䇑ᆖᱮ㪇ᐞᔲн ާᴹ⭏⢙ᆖ᜿ѹǄަԆⲴሩ∄ѝˈ൘ἽṚሩ䶎⭏⢙㛱䘛৽ᓄ˄ᙫޡ 172 㓴ᮠᦞሩ ∄˅ǃ⯵ᇣ䙐ᡀⲴᦏᇣ˄ޡ 210 㓴ᮠᦞሩ∄˅ԕ৺㢲㛒ࣘ⢙䙐ᡀডᇣ˄ޡ 167 㓴 ᮠᦞሩ∄˅Ⲵ䇴ՠᯩ䶒ˈMON 87427 ઼䶎䖜สഐሩ➗䰤⋑ᴹ㔏䇑ᆖᱮ㪇ᐞᔲǄ ↔ཆˈ൘⦹㊣ཌ㴮઼⅗⍢⦹㊣㷏ডᇣⲴ䇴ՠᯩ䶒ˈMON 87427 ઼ሩ➗䰤ҏнᆈ ൘㔏䇑ᆖᱮ㪇ᐞᔲǄ䘉Ӌ㔃᷌ҏ䘋а↕㺘᰾ˈMON 87427 нՊ໎࣐֌⢙ᵲ㥹ॆ Ǆੁٮᡆ䖜ਈѪᴹᇣ⭏⢙Ⲵ ൘ᴹᇣ㢲㛒ࣘ⢙઼ᴹ⳺㢲㛒ࣘ⢙ⲴѠᓖ䇴ՠѝˈሩ 4 њ䈅傼⛩Ⲵᮠᦞᙫޡ䘋㹼Ҷ 203 㓴ሩ∄˄98 㓴ᴹᇣ㢲㛒ࣘ⢙ᮠᦞሩ∄઼ 105 㓴ᴹ⳺㢲㛒ࣘ⢙ᮠᦞሩ∄˅ˈ ަѝ 191 㓴ᮠᦞሩ∄ MON 87427 ઼䶎䖜สഐሩ➗䰤⋑ᴹ㔏䇑ᆖᱮ㪇ᐞᔲǄ൘ 12 㓴ᆈ൘㔏䇑ᆖᐞᔲⲴᮠᦞሩ∄ѝˈᴹ 6 㓴ሩ∄ѝ MON 87427 Ⲵᴹᇣᡆᴹ⳺㢲㛒 ࣘ⢙ѠᓖⲴᒣ൷٬൘୶ъॆᑨ㿴૱⿽Ⲵᮠ٬㤳ത޵˗ᴹ 6 㓴ᮠᦞሩ∄ѝ MON 87427 Ⲵᒣ൷٬㲭❦䎵ࠪ୶ъॆᑨ㿴૱⿽Ⲵᒣ൷٬㤳തˈնᱟ䘉Ӌᐞᔲ൘ н਼䈅傼⛩䰤㺘⧠ᒦна㠤ˈഐ↔нާᴹ⭏⢙ᆖ᜿ѹǄ㔬ਸཊњ䈅傼⛩ᮠᦞⴻᶕˈ ˈੁٮоᑨ㿴⦹㊣⴨∄ˈMON 87427 нՊ໎࣐֌⢙ᵲ㥹ॆᡆ䖜ਈѪᴹᇣ⭏⢙Ⲵ нՊ᭩ਈަሩ䶎⭏⢙㛱䘛ǃ⯵ᇣᡆ㢲㛒ࣘ⢙Ⲵ৽ᓄ઼㙀ਇᙗˈнՊሩ⧟ຳӗ⭏ᱮ 㪇ᖡ૽Ǆ ਇߌъ䜘Ⲵငᢈˈߌъ䜘䖜สഐἽ⢙⧟ຳᆹޘⴁⶓỰ傼⍻䈅ѝᗳ˄䮯᱕˅᳘ ਹ᷇ⴱߌъ、ᆖ䲒Ҿ 2014 ᒤᆼᡀҶሩ MON 87427 ᣇ䲔㥹ࡲ⦹㊣Ⲵ⧟ຳᆹޘᙗ䇴 ՠǄ㔃᷌㺘᰾ˈ䖜สഐᣇ䲔㥹ࡲ⦹㊣ MON87427 ሩ㠣ቁ 2 ؽ᧘㦀ࡲ䟿௧ᯭⲴߌ 䗮䲔㥹ࡲާᴹ㢟ྭⲴ㙀ਇᙗǄ൘㦂ൠ઼ṭษൠ䈅傼ᶑԦлˈ䖜สഐ⦹㊣ MON87427 Ⲵ⭏ᆈㄎҹ㜭࣋оަሩᓄⲴ䶎䖜สഐ⦹㊣൷ᰐᱮ㪇ᐞᔲǄо䶎䖜สഐ ⦹㊣⴨∄ˈ䖜สഐ⦹㊣ MON87427 ሩ⭠䰤ѫ㾱㢲㛒ࣘ⢙ᮠ䟿઼㗔㩭㔃ᶴǃѫ㾱匎㗵ⴞᇣ㲛ԕ৺ѫ㾱⯵ᇣᰐᱮ㪇ᖡ૽Ǆ 㔬кᡰ䘠ˈMON 87427 ᱟᯠⲴ⦹㊣ࡦ⿽઼⿽ᆀ⭏ӗ㌫㔏ѝ䶎ᑨ䟽㾱Ⲵањ 䇴ԧ䍴ᯉ㺘᰾ˈMON 87427 䖜ॆ⭘Ⲵ䚇Ր㺘䗮о䈳᧗ݳԦᱟޘ㢲ˈབྷ䟿Ⲵᆹ⧟ ᆹޘⲴˈᒦф䖜ॆᯩ⌅ҏнՊ᭩ਈਇփⲴᆹޘᙗ˗ཆⓀสഐѪঅњս⛩Ⲵঅᤧ䍍 ᓧڕ⢙ф䚇Ր㺘⧠っᇊ˗MON 87427 㺘䗮Ⲵ CP4 EPSPS 㳻ⲭሩӪ㊫઼ࣘˈޕᨂ ⋑ᴹн㢟ᖡ૽ˈҏн኎Ҿ㠤᭿ᡆ∂ᙗ㳻ⲭ˗MON 87427 ൘ᡀ࠶коᑨ㿴ሩ➗૱ ⧟ሩ⭏ᘱˈੁٮ⿽⦹㊣ᱟᇎ䍘ㅹ਼Ⲵ˗MON 87427 ҏнާᴹ䖜ਈѪᴹᇣ⭏⢙Ⲵ ຳ⋑ᴹн࡙ᖡ૽ǄMON 87427 ⴞࡽᐢ㓿൘࣐᤯བྷ઼㖾ഭ㧧ᗇ୶ъॆ⿽Ἵᢩ߶ˈ ǃਠ⒮ǃᰕᵜǃ丙ഭǃକՖ∄ӊǃ㨢ᖻᇮǃޠᒦݸਾ൘໘㾯କǃ◣བྷ࡙ӊ/ᯠ㾯䎺ইǃ⅗ⴏㅹഭᇦ઼ൠ४㧧ᗇ䘋ਓᢩ߶Ǆ ᆏኡ䜭ޜਨᲲᰦ⋑ᴹ䇑ࡂ൘ѝഭ⭣䈧⿽Ἵ MON 87427 ⦹㊣ˈᵜ⭣䈧ӵѪҶ 㧧ᗇਟԕ⭘֌࣐ᐕ৏ᯉⲴᣇ䲔㥹ࡲ⦹㊣ MON 87427 ৺ަ࣐ᐕӗ૱Ⲵᆹޘ䇱ҖǄ 䢤Ҿ MON 87427 ᐢ㓿㧧ᗇ㖾ഭ઼࣐᤯བྷⲴ୶ъॆ⿽Ἵ䇨ਟˈѪҶнᖡ૽⦹㊣ӗ ૱䘋ਓˈᡆᱟ⭡Ҿ䖜สഐ⦹㊣վ≤ᒣ␧ᵲᑖᶕⲴ䍨᱃ѝᯝ仾䲙ˈᆏኡ䜭䘌ьᴹ䲀 ޜਨ᤹➗ѝॾӪ≁ޡ઼ഭߌъ䜘Ⲵ⴨ޣ⌅㿴㾱≲ˈ⢩↔ᨀࠪ⭣䈧ˈ䈧≲㧧ᗇᣇ㥹 ⭈㟖䲔㥹ࡲ䖜สഐ⦹㊣ MON 87427 䘋ਓ⭘֌࣐ᐕ৏ᯉⲴᆹޘ䇱ҖˈᵋҸԕᢩ߶Ǆ

11 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 йǃᐕ֌ⴞⲴ઼᜿ѹ ⦹㊣ࡦ⿽઼⿽ᆀ⭏ӗᱟ⦹㊣⭏ӗⲴањ䶎ᑨ䟽Ⲵ⧟㢲Ǆ䙊ᑨᛵߥл⦹㊣ᵲӔ ⿽ࡦ⿽⭠㾱᤹аᇊ∄ֻ⿽Ἵ⡦⇽ᵜˈᒦфሩ⇽ᵜ৫䳴Ǆ৫䳴ᱟ੖ᖫᓅԕ৺৫䳴ᱟ ੖৺ᰦሩ⿽ᆀ䍘䟿ᴹᖸབྷᖡ૽Ǆ਼ᰦˈ৫䳴ҏ㾱㙇䍩䖳ཊӪᐕǄӪԜҏᴮ䈅മ䟷 ⭘䳴ᙗн㛢㌫ᢰᵟᶕࡦ⿽ˈቭ㇑ҏᴹ⴨ᖃ㿴⁑Ⲵᓄ⭘ˈնࡠⴞࡽѪ→࡙⭘䳴ᙗн 㛢ᢰᵟࡦ⿽࿻㓸ᆈ൘⵰䘉ṧ઼䛓ṧⲴ䰞仈Ǆഐ↔ˈራ᢮ᯠⲴࡦ⿽䙄ᖴ઼ᢰᵟаⴤ ӪԜⲴⴞḷǄ MON 87427 ᱟ䙊䗷䖜สഐᢰᵟሬޕ cp4 epsps สഐ㧧ᗇⲴǄcp4 epsps สഐਟ ԕ㕆⸱ CP4 EPSPS 㳻ⲭǄն⭡Ҿ MON 87427 Ⲵสഐ㺘䗮ⴂѝ䟷⭘ањ⢩↺Ⲵ੟ ࣘᆀ઼޵ਜ਼ᆀ㓴ਸ˄e35S-hsp70˅ᶕ䈳᧗ CP4 EPSPS Ⲵ㺘䗮ˈᡰԕ MON 87427 ѝⲴ CP4 EPSPS 㳻ⲭ㺘䗮ᴹ㓴㓷⢩ᔲᙗǄCP4 EPSPS 㳻ⲭ൘ MON 87427 Ⲵ㩕ޫ 㓴㓷ಘᇈ઼䳼ᙗ⭏⇆㓴㓷ಘᇈѝਟԕ↓ᑨ㺘䗮ˈն൘䳴ᙗ⭏⇆㓴㓷ಘᇈѝн㺘䗮 ᡆ㺘䗮䟿ᖸվǄ࡙⭘䘉а⢩ᙗˈ㔃ਸ௧ᯭ㥹⭈㟖䲔㥹ࡲˈਟԕ൘ࡦ⿽䗷〻ѝӪᐕ ࡋ䙐䳴ᙗн㛢Ǆ ⦹㊣Ⲵ䳴ょਁ㛢࿻Ҿ㩕ޫ⭏䮯䱦⇥Ⲵ V9 ᵏˈᒦф CP4 EPSPS 㳻ⲭ൘ MON 87427 Ⲵ䳴ᙗಘᇈѝн㺘䗮ᡆ㺘䗮䟿ᖸվˈഐ↔൘ MON 87427 ⭏䮯ਁ㛢Ⲵ V8-V13 䱦⇥௧ᯭ㥹⭈㟖䲔㥹ࡲਟԕᵰ↫㣡㊹⇽㓶㜎Ǆ࡙⭘ਜ਼ᴹ MON 87427 䖜ॆ փⲴ㠚Ӕ㌫֌⇽ᵜˈ⭘ਜ਼ᴹަᆳᣇ㥹⭈㟖䖜ॆփⲴ㠚Ӕ㌫֌⡦ᵜˈ൘⦹㊣Ⲵ䘲ᇌ ⭏䮯䱦⇥௧ᯭ㥹⭈㟖ˈਟԕᶴᡀӪᐕн㛢㌫ࡦ⿽㌫㔏Ǆ ࡙⭘ MON 87427 䘋㹼⦹㊣ࡦ⿽઼⿽ᆀ⭏ӗˈާᴹԕл᜿ѹ˖ 1˅໎࣐ᵲӔ⿽ࡦ⿽Ⲵ⚥⍫ᙗ˖⇽ᵜ৫䳴Ⲵޣ䭞ᰦᵏᱟӾ䳴ょᣭࠪࡠᮓ㊹䘉 ⇥ᰦᵏˈᒣ൷Ѫ 3-4 ཙǄᖃࡽӪᐕ৫䳴Ⲵᯩ⌅वᤜᵪỠᣭ䳴˄а㡜㾱 2 ⅑˅઼᡻ᐕᣭ䳴˄а㡜 3 ⅑˅Ǆн䇪ଚ⿽ᯩ⌅ˈ䜭䴰㾱൘ 3-4 ཙⲴᰦ䰤䟼䳶ѝ䏣ཏⲴӪ઼࣋䇮༷Ǆ㘼䟷⭘ MON 87427 ࡦ⿽ˈਚ䴰㾱൘ V8-V13˄བྷ 㓖 14 ཙ˅Ⲵᰦ䰤޵௧⍂є⅑㥹⭈㟖䲔㥹ࡲǄഐ↔ˈ⦹㊣ࡦ⿽Ⲵ⚥⍫ᙗᴤ 儈Ǆ 2˅߿ቁᵲӔ⿽ࡦ⿽Ⲵᡀᵜ˖䲿⵰㓿⍾ਁኅˈ䎺ᶕ䎺ཊߌъӪਓ䖜ੁ෾ᐲˈ ᒦфࣣࣘ࣋ᡀᵜⵏ൘нᯝкॷˈ䟷⭘Ӫᐕ৫䳴Ⲵᯩ⌅ሶՊ䶒Ѥ⭘ᐕ⸝㕪 ઼ᵲӔ⿽⿽ᆀᡀᵜ⴨ሩ䖳儈Ⲵ᥁ᡈǄ㘼䟷⭘ MON 87427 ࡦ⿽㌫㔏Պᴹ ࣙҾ䀓ߣ䘉Ӌ䰞仈Ǆ ⴞࡽˈᆏኡ䜭ޜਨ⋑ᴹ䇑ࡂ൘ѝഭ୶ъॆ⿽Ἵ MON 87427Ǆ䢤Ҿ MON 87427 ᐢ㓿㧧ᗇ㖾ഭ઼࣐᤯བྷⲴ୶ъॆ⿽Ἵ䇨ਟˈѪҶнᖡ૽⦹㊣ӗ૱䘋ਓˈᡆᱟ⭡Ҿ 䖜สഐ⦹㊣վ≤ᒣ␧ᵲᑖᶕⲴ䍨᱃ѝᯝ仾䲙ˈᆏኡ䜭䘌ьᴹ䲀ޜਨ᤹➗ѝॾӪ≁ ޡ઼ഭߌъ䜘Ⲵ⴨ޣ⌅㿴㾱≲ˈ⢩↔ᨀࠪ⭣䈧ˈ䈧≲㧧ᗇᣇ㥹⭈㟖䲔㥹ࡲ䖜สഐ ⦹㊣ MON 87427 䘋ਓ⭘֌࣐ᐕ৏ᯉⲴᆹޘ䇱ҖˈᵋҸԕᢩ߶Ǆ

12 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ഋǃഭ޵ཆ⴨ޣ⹄ウⲴ㛼Ჟ䍴ᯉ ޘ⨳䖜สഐ֌⢙৺䖜สഐ⦹㊣ᓄ⭘ᛵߥ Ӿ 1996 ᒤࡠ 2017 ᒤˈ䖜สഐ֌⢙୶ъॆᐢ䎠䗷Ҷ 21 ᒤǄ൘ 2016 ᒤˈ26 њഭᇦ⿽ἽҶ 1.851 ӯޜ产Ⲵ䖜สഐ֌⢙ˈ∄ 2015 ᒤⲴ 1.797 ӯޜ产໎䮯Ҷ 3ˁ˗ ަѝ 19 њѪਁኅѝഭᇦˈ7 њѪਁ䗮ഭᇦ˗ਁኅѝഭᇦⲴ⿽Ἵ䶒〟ঐޘ⨳䖜ส ഐ֌⢙⿽Ἵ䶒〟˄1.851 ӯޜ产˅Ⲵ 54%ˈ㘼ਁ䗮ഭᇦⲴ⿽Ἵ䶒〟ঐ 46%ǄӾ 1996 ᒤⲴ 170 зޜ产ࡠ 2016 ᒤⲴ 1.85 ӯޜ产ˈޘ⨳䖜สഐ֌⢙Ⲵ⿽Ἵ䶒〟໎࣐໎䮯 ߌ≁Ӿѝਇ⳺䎵䗷 1500 ӯ㖾ݳ⨳ޘˈҶ 110 ؽ˄ISAAAϦ2017˅ˈ20 ᒤԕᶕ ˄ISAAAϦ2017˅Ǆ㖾ഭǃᐤ㾯ǃ䱯ṩᔧǃ࣐᤯བྷ઼ঠᓖӽ❦ᱟᧂ਽ࡽӄⲴᴰѫ 㾱䖜สഐ֌⢙⿽Ἵഭᇦˈਸ䇑⿽Ἵ䶒〟ঐࡠҶ 91%Ǆ䲔↔ѻཆˈ᣹㖾 10 ഭⲴ⿽ Ἵ䶒〟ޡ䇑Ѫ 8000 зޜ产ˈӊཚൠ४ 8 ഭ⿽Ἵ䶒〟ޡ䇑Ѫ 1860 зޜ产ˈ⅗ⴏ 4 ޜഭ˄㾯⨝⢉ǃ㪑㨴⢉ǃᯟ⍋Հݻǃᦧݻ˅Ⲵ䖜สഐ⦹㊣⿽Ἵ䶒〟䗮ࡠ 13.6 з 产ˈই䶎઼㣿ѩҏ໎࣐Ҷ䖜สഐ֌⢙Ⲵ⿽Ἵ䶒〟˄ISAAAϦ2017˅Ǆ ༽ਸᙗ⣦ӗ૱ᱟ䘁ᒤᶕ໎䮯ᴰᘛⲴа㊫ᙗ⣦ˈ㲭❦ 2016 ᒤ㙀䲔㥹ࡲ֌⢙Ⲵ ⿽Ἵ䶒〟ӽঐޘ⨳䖜สഐ֌⢙⿽Ἵ䶒〟Ⲵ 47%˄8650 зޜ产˅ˈնਜ਼ᴹє⿽ᡆ ԕкᙗ⣦Ⲵ༽ਸᙗ⣦䖜สഐ֌⢙⿽Ἵ䶒〟ᐢ䗮ࡠ 7540 зޜ产ˈঐᙫ䖜สഐ֌⢙ ⿽Ἵ䶒〟Ⲵ 41%ˈ∄ 2015 ᒤ໎࣐ 29%Ǆ㠚 2014 ᒤᔰ࿻ˈ᣹б㖾⍢Ⲵᐤ㾯ǃ䱯 ṩᔧǃᐤ᣹൝઼Ѽ᣹൝䱶㔝ᔰ࿻⿽Ἵᣇ㲛㙀䲔㥹ࡲⲴ༽ਸᙗ⣦བྷ䉶Ǆቔަᱟᐤ㾯ˈ ᣇ㲛㙀䲔㥹ࡲⲴ Intacta བྷ䉶ഐѪ㢲㓖ߌ㦟઼䘲⭘ݽ㙅ᢰᵟ㘼൘ߌ≁ѝབྷਇ⅒䗾Ǆ 䖜สഐ⦹㊣ᱟӵ⅑Ҿབྷ䉶ⲴㅜҼབྷ䖜สഐ֌⢙ˈ2016 ᒤ⿽Ἵ䶒〟ঐޘ⨳⦹ ㊣ᙫ⿽Ἵ䶒〟Ⲵ 26%Ǆ䖜สഐ⦹㊣ԕᣇ㲛ᣇ䲔㥹ࡲ༽ਸᙗ⣦ѪѫˈቔަᱟӾ 2010 ᒤᔰ࿻ˈਜ਼ᴹ 8 њสഐǃ3 ⿽ᙗ⣦Ⲵ SmartStaxTM ༽ਸᙗ⣦⦹㊣䘋ޕᐲ൪ˈᑞࣙ ߌ≁ᴤᴹ᭸ൠ䱢⋫ൠк઼ൠлᇣ㲛ˈ਼ᰦҏᨀ׋ᴹ᭸Ⲵᵲ㥹⋫⨶᡻⇥Ǆ䲿⵰䎺ই Ⲵ࣐ޕˈ2016 ᒤޘ⨳ᴹ 17 њഭᇦᢩ߶୶ъॆ⿽Ἵ䖜สഐ⦹㊣˄ISAAAϦ2017˅Ǆ 2016 ᒤᱟ᜿ѹ䶎ࠑⲴаᒤˈ䘉аᒤˈ䈪䍍ቄ྆㧧ᗇ㘵Ԝㅜа⅑ਁ㺘Ҷа亩 ༠᰾ˈ᭟ᤱ⭏⢙ᢰᵟˈ䚓䍓ԕ੩∋≲⯥Ⲵုᘱ৽ሩ䘉亩ᢰᵟ઼哴䠁བྷ㊣Ⲵᢩ䇴㘵Ǆ 䖜ԕ৺ਟᤱ㔝ߌъ 2030ٿ㚄ਸഭ㋞伏оߌъ㓴㓷ǃഭ䱵伏⢙о᭯ㆆ⹄ウᡰǃ20 ഭ 䇞〻ᤷሬлⲴަᆳ㊫լᵪᶴ൷㠤࣋Ҿ൘ 15 ᒤᡆ㘵ᴤ⸝Ⲵᰦ䰤޵䀓ߣ侕侯઼㩕ޫ 䰞仈Ǆᴤ䟽㾱Ⲵᱟˈ㖾ഭഭᇦ、ᆖ䲒ǃഭᇦᐕ〻䲒઼ഭᇦ५ᆖ䲒ਜ䳶Ҷ 50 ཊս 、ᆖᇦǃ⹄ウӪઈǃߌъ઼ᐕъуᇦˈ㓴ᡀᣕ੺㕆߉ငઈՊˈ൘䮯䗮єᒤⲴ㕆߉ ⹄ウˈ֯⭘Ҷ㠚䖜สഐ֌⢙俆⅑ਁ᰾ԕᶕǃ㠣Ӻ 20ޣ䗷〻ѝ৲㘳Ҷ 900 ཊ亩⴨ ᒤ䰤〟㍟ⲴᮠᦞǄ2016 ᒤ 5 ᴸ 19 ᰕˈ䈕уᇦငઈՊਁᐳᣕ੺ǉสഐᐕ〻֌⢙˖ 㓿傼оኅᵋǊ〠ˈ䙊䗷สഐᐕ〻⿽ἽⲴߌ֌⢙ਟԕᆹޘൠ伏⭘ˈнՊ໎࣐ᛓⱼ⯷ǃ 㛕㜆ǃ㛳㛐⯮⯵ǃ㛮⯵ǃ㠚䰝⯷ᡆ䗷᭿Ⲵࠐ⦷˄https://nas-sites.org/ge-crops/˅Ǆ аઘਾˈ㤡ഭⲷᇦᆖՊҏࠪ⡸ᇓՐ޼〠ˈоՐ㔏ߌ֌⢙⴨∄ˈ䖜สഐߌ֌⢙нՊ ᓧ䙐ᡀᴤབྷডᇣǄ䈕ሿ޼ᆀᱟ⭡㤡ഭ 6 ਽䍴␡Ἵ⢙ᆖᇦ㓴ڕ⢙ሩ⧟ຳᡆӪ㊫઼ࣘ ᡀⲴуᇦ㓴ᆼᡀǄᣕ੺ᤷࠪˈ“⋑ᴹ䇱ᦞ㺘᰾伏⭘а⿽ߌ֌⢙ᱟড䲙Ⲵ——ӵӵ ᱟഐѪᆳᱟ䖜สഐ伏૱”Ǆ“㠚 18 ᒤࡽ䖜สഐߌ֌⢙⭏ӗㅜа⅑བྷ㿴⁑୶ъॆԕ ᶕˈ⋑ᴹԫօ䇱ᦞ䇱᰾ˈаӋн㢟ਾ઼᷌⎸䍩ԫօа⿽㓿ᢩ߶Ⲵ䖜สഐߌ֌⢙ѻ

13 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䰤ᆈ൘ޣ㚄Ǆ”ᣕ੺䘈ᤷࠪˈ䖜สഐߌ֌⢙⋑ᴹሩ⧟ຳᶴᡀԫօ⢩↺Ⲵডᇣ ˄https://royalsociety.org/~/media/policy/projects/gm-plants/gm-plant-q-and-a.pdf˅Ǆ ∄˄䖜สഐ֌⢙Ⲵᐲ൪ԧ٬Ѫ 158 ӯ㖾ݳ⨳ޘᦞ Cropnosis ᵪᶴՠ㇇ˈ2016 ᒤ ⨳ ޘˈᣔᐲ൪ 735 ӯ㖾ݳᐲ٬Ⲵ 22%؍⢙֌⨳ޘᒤ໎䮯Ҷ 3%˅ˈঐ 2016 ᒤ 2015 ᐢ᭦㧧Ⲵ“ᴰ㓸ӗ૱”˄䖜สഐ֌⨳ޘ୶ъ⿽ᆀᐲ൪ 45 ӯ㖾ݳᐲ٬Ⲵ 35%Ǆ亴ᵏ ᱟ䖜สഐ⿽ᆀঅ⤜ԧ٬Ⲵ 10 ؽԕкǄޕᆳ᭦㧧Ⲵӗ૱˅Ⲵߌ൪ࠪ൪᭦ަ઼⢙ Klumper ઼ Qaim˄2014˅࡙⭘൘ц⭼਴ൠ䘋㹼Ⲵߌ൪䈳ḕᡆ⭠䰤䈅傼ᗇࠪⲴ৏ ࿻ᮠᦞሩ䗷৫ 20 ᒤޘ⨳ 147 亩ᐢ⸕Ⲵ䖜สഐ֌⢙⹄ウ䘋㹼Ҷѕ䉘Ⲵ㔬ਸ࠶᷀ˈ ᒦᣕ੺Ҷ䖜สഐབྷ䉶ǃ⦹㊣઼ỹ㣡ሩ֌⢙ӗ䟿ǃߌ㦟֯⭘઼ߌ≁᭦⳺ᯩ䶒Ⲵᖡ૽Ǆ 䈕㔬ਸ࠶᷀Ⲵ㔃䇪ᱟĀ䖜สഐ֌⢙൘ 1995 ᒤ㠣 2014 ᒤ䰤ӗ⭏Ҷཊ䟽䟽བྷ᭸⳺˖ ≁䟷⭘䖜สഐᢰᵟ֯ॆᆖߌ㦟Ⲵ֯⭘⦷䱽վҶ 37ˁˈ֌⢙ӗ䟿ᨀ儈Ҷ 22ˁˈߌ ࡙⏖໎࣐Ҷ 68ˁǄᣇ㲛֌⢙Ⲵӗ䟿໎⳺઼ߌ㦟߿ቁⲴ〻ᓖབྷҾᣇ䲔㥹ࡲ֌⢙Ǆ ਁኅѝഭᇦ∄ਁ䗮ഭᇦⲴӗ䟿઼࡙⏖໎⳺儈Ǆā䈕㔃䇪ঠ䇱ҶԕᖰⲴޘ⨳ᒤᓖ⹄ ウᗇࠪⲴа㠤ᙗ㔃᷌˖Ӿ 1996 ᒤࡠ 2013 ᒤ䰤֌⢙ӗ䟿໎࣐Ⲵՠ㇇ԧ٬Ѫ 1333 ӯ㖾ݳˈަѝˈਁ䗮ഭᇦ㧧ᗇⲴ㍟䇑㓿⍾᭸⳺㓖Ѫ 652 ӯ㖾ݳˈਁኅѝഭᇦӗ⭏ Ⲵ㓿⍾᭸⳺㓖Ѫ 681 ӯ㖾ݳ˄Brookes and Barfoot, 2015˅Ǆ Ӿ 1994 ᒤሿ㿴⁑⿽Ἵࡠ 2016 ᒤˈޘ⨳ޡᴹ 40 њഭᇦ઼ൠ४˄39 њഭᇦ৺ ⅗ⴏ 28 ഭ˅ᢩ߶Ҷཊњ䖜สഐӗ૱䘋ਓ⭘Ҿ伏⭘ǃ侢⭘ᡆ⧟ຳ䟺᭮Ǆ㠚 1994 ᒤԕᶕˈ䘉Ӌഭᇦޡ䇑ሩ 26 ⿽Ἵ⢙˄нवᤜᓧѳ俘ǃ⧛⪠ǃ⸞⢥⢋ㅹ㿲䍿Ἵ⢙˅ Ⲵ 392 њ䖜ॆһԦᆼᡀҶ 3768 亩⅑⌅㿴ᢩ߶Ǆަѝˈ1777 亩ᢩ߶Ѫ伏⭘⭘䙄˄ⴤ ᧕֯⭘ᡆ⭘Ҿ࣐ᐕ˅ǃ1238 亩Ѫ侢⭘⭘䙄˄ⴤ᧕֯⭘ᡆ⭘Ҿ࣐ᐕ˅ǃ753 亩Ѫ⧟ຳ 䟺᭮ᡆ⿽Ἵᢩ߶˄ISAAAϦ2017˅Ǆ⦹㊣ӽ❦ᱟ䖜ॆփ㧧ᢩᮠ䟿ᴰཊⲴ֌⢙˄29 њഭᇦ/ൠ४Ⲵ 218 њ䖜ॆփᢩ߶˅ˈަ⅑ᱟỹ㣡ǃ傜䫳㯟ǃ⋩㨌઼བྷ䉶Ǆ

CP4 EPSPS 㳻ⲭ৺ᣇ㥹⭈㟖䲔㥹ࡲ䖜สഐ֌⢙Ⲵ⹄ウ䘋ኅ 5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦˄EPSPS˅ᒯ⌋ᆈ൘ҾἽ⢙઼ᗞ⭏⢙փ޵ˈ 䭞䞦ˈ㘼㧭㥹䞨䙄ᖴ䍏䍓൘Ἵ⢙઼ᗞ⭏⢙փ޵ᨀ׋㣣俉ޣᱟ㧭㥹䞨䙄ᖴѝⲴањ ⧟ˈ⭘Ҿ㣣俉᯿≘ส䞨˄㤟щ≘䞨ǃ䞚≘䞨ǃ㢢≘䞨˅઼䇨ཊ⅑㓗⢙˄ྲഋ≒ਦ 䞨ǃ⌋䞼ǃ㔤⭏㍐ Kǃ㣡䶂㍐ǃ㊫哴䞞ǃᵘ䍘㍐ㅹ˅Ⲵ⭏⢙ਸᡀ˄Herrmann and Weaver 1999; Maeda and Dudareva 2012˅Ǆ䈕䙊䐟൘Ἵ⢙փ޵䶎ᑨ䟽㾱ˈἽ⢙փ㠣 ቁ 35%Ⲵᒢ⢙䍘ᶕⓀҾ㧭㥹䞨䙄ᖴⲴӗ⢙˄Franz et al. 1997˅Ǆྲമ 1 ᡰ⽪ˈEPSPS ✟䞦֌⭘Ҿ㧭㥹䞨䙄ᖴѝⲴㅜ 6 ↕ˈ䍏䍓ۜॆሶ⼧䞨✟䞷ᔿщ䞞䞨˄PEP˅кⲴ 䞷ᔿщ䞞䞠ส䖜〫ࡠ㧭㥹䞨-3-⼧䞨˄S3P˅кˈ৽ᓄ⭏ᡀ 5-✟䞷ᔿщ䞞䞠㧭㥹䞨 2- -3-⼧䞨˄EPSP˅ˈ਼ᰦ䟺᭮ࠪᰐᵪ⼧䞨˄HPO4 ˅Ǆ˄Alibhai and Stallings, 2001˅Ǆ

14 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 PEP

Inhibitor:

Shikimate-3-phosphate Glyphosate 5-Enolpyruvyl-shikimate-3-phosphate S3P EPSP മ 1. EPSPS 䞦ۜॆ৽ᓄ⽪᜿മ

˅EPSPS 䞦֌⭘Ҿ㧭㥹䞨䙄ᖴѝⲴㅜ 6 ↕ˈ䍏䍓ۜॆᓅ⢙㧭㥹䞨-3-⼧䞨˄S3P о✟䞷ᔿщ䞞䞨˄PEP˅㔃ਸˈ⭏ᡀ 5-✟䞷ᔿщ䞞䞠㧭㥹䞨-3-⼧䞨˄EPSP˅ˈ਼ᰦ 2- 䟺᭮ࠪᰐᵪ⼧䞨˄HPO4 ˅Ǆ㥹⭈㟖˄Glyphosate˅ᱟ PEP Ⲵㄎҹᙗᣁࡦ⢙ˈ㥹⭈㟖 Ⲵᆈ൘ਟ䱫→ EPSPS 䞦ሩ PEP Ⲵ࡙⭘ˈ䱫ᯝἽ⢙փ޵㣣俉᯿≘ส䞨ⲴਸᡀˈӾ㘼ਁ ᥕ䲔㥹֌⭘Ǆ

ԕ㥹⭈㟖Ѫᴹ᭸ᡀ࠶Ⲵ䲔㥹ࡲߌ䗮®ᱟа㊫䶎䘹ᤙᙗ䲔㥹ࡲˈ㥹⭈㟖ਟԕᣁ ࡦἽ⢙޵ⓀⲴ EPSPS 䞦⍫ᙗˈӾ㘼䱫ᯝἽ⢙⭏䮯ᡰᗵ䴰Ⲵ㣣俉᯿≘ส䞨Ⲵ⭏⢙ ਸᡀˈሬ㠤Ἵ⢙↫ӑˈᱟ䶎ᑨᴹ᭸Ⲵ䲔㥹ࡲˈ⭘Ҿ䱢⋫аᒤ⭏ᡆཊᒤ⭏Ⲵঅᆀਦ ᵲ㥹઼䱄ਦ㊫ᵲ㥹Ǆ❦㘼㥹⭈㟖኎Ҿ䶎䘹ᤙᙗ䲔㥹ࡲˈ൘䱢⋫ᵲ㥹Ⲵ਼ᰦ䘈Պᵰ ↫ߌ֌⢙Ǆഐ↔ˈᣇ㥹⭈㟖֌⢙Ⲵᓄ⭘ሩߌъ⭏ӗ⍫ࣘާᴹ䶎ᑨ䟽㾱Ⲵ᜿ѹǄ俆 ݸˈᣇ㥹⭈㟖֌⢙Ⲵᓄ⭘ਟԕѪߌ≁ᑖᶕа⿽ᒯ䉡ᵲ㥹䱢䲔᧚ᯭ˗ㅜҼˈ㥹⭈㟖 ˗ᱟа⿽⧟ຳ৻ྭරⲴ䲔㥹ࡲ˗ㅜйˈਟԕ֯㥹⭈㟖㊫䲔㥹ࡲ൘֌⢙⭏䮯ᵏᓄ⭘ ˈㅜഋˈᣇ䲔㥹ࡲ֌⢙઼䲔㥹ࡲ㓴ᡀⲴᵲ㥹䱢䲔㌫㔏ਟԕ᧘ᒯቁ㙅ᡆݽ㙅㙅֌ᯩᔿ 㲰઼㢲ⴱ㜭Ⓚ˗ㅜӄˈᨀ׋վ㙇儈᭸Ⲵᵲ㥹䱢䲔ץᤱ൏༔≤࠶ǃ߿ቁ൏༔؍Ӿ㘼 ᢰᵟǄ 、ᆖᇦ䮯ᵏԕᶕаⴤ㠤࣋ษ㛢ᣇ䲔㥹ࡲⲴ֌⢙૱⿽ˈն䟷⭘Ր㔏Ⲵ㛢⿽ᢰᵟ ˄ྲՐ㔏Ⲵ䈡ਈ઼䘹ᤙᢰᵟ˅䜭ᵚਆᗇᡀ࣏˄OECD, 1999˅ǄӾкц㓚 80 ᒤԓ ,ᔰ࿻ˈӪԜᔰ࿻ُࣙҾ䟽㓴 DNA ᢰᵟᶕᔰਁᣇ㥹⭈㟖֌⢙ˈᒦ㧧ᗇᡀ࣏˄Gasser 1989; Hinchee et al., 1993; Padgette et al., 1989; 1995; Mazur, 1989; Kishore and ֯׳Shah, 1988; Kishore et al., 1988˅Ǆѫ㾱䟷⭘Ⲵ৏⨶ᡆᵪࡦᴹ 3 њᯩ䶒˖аᱟ ޵Ⓚ EPSPS 䎵䟿㺘䗮˄Shah et al., 1986˅˗Ҽᱟሬޕањо㥹⭈㟖Ӣ઼ᙗվⲴཆ Ⓚ EPSPS สഐˈᶴᔪਖањԓ䉒䙊䚃˄Barry et al., 1992; Comai et al., 1983; Hinchee et al., 1993˅˗йᱟሬޕањਟ䱽䀓㥹⭈㟖Ⲵสഐ˄Barry et al., 1992˅Ǆ ᣇ䲔㥹ࡲ⦹㊣ MON 87427 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭ↓ᱟ࡙⭘Ҷањо㥹⭈ ㊣޵Ⓚᙗ⦹گ㟖Ӣ઼ᙗվⲴ EPSPS 䞦ˈ൘㥹⭈㟖ᆈ൘ᰦˈCP4 EPSPS 㜭ཏԓ EPSPS 䞦Ⲵ࣏㜭ˈᤱ㔝ᨀ׋Ἵ⢙փ⭏䮯ਁ㛢ᡰᗵ䴰Ⲵ㣣俉᯿≘ส䞨ࡽփǄ CP4 EPSPS ᴰࡍᱟӾ൏༔ѝᑨ㿱Ⲵߌᵶ㧼˄Agrobacterium˅CP4 㧼Ṛѝ࠶⿫ ᗇࡠⲴˈަоᣁࡦ⢙㥹⭈⼧Ⲵ㔃ਸᑨᮠ appKi[㥹⭈㟖]Ѫ 2.7 mMˈ㘼оཙ❦ᓅ⢙ PEP Ⲵ㔃ਸᑨᮠ appKm[PEP]Ѫ 12 μM˄Barry et al., 1992; Padgette et al., 1995a˅˗ 15 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 appK ᱟ䞦оḀ⿽ॆਸ⢙Ⲵ㔃ਸᑨᮠˈᮠ٬䎺བྷˈ㺘᰾䈕ॆਸ⢙о䞦ਁ⭏㔃ਸᡰ 䴰㾱Ⲵ⎃ᓖ䎺བྷˈণ䞦о䈕ॆਸ⢙Ⲵ㔃ਸ㜭࣋䎺ᕡǄCP4 EPSPS Ⲵ appK1[㥹⭈ ণ㥹⭈㟖ᆈ൘ᶑԦл CP4 EPSPS ӽˈ٬ᱟ᮷⥞ᣕ䚃ѝⲴᴰ儈٬∄[㟖]/appKm[PEP Ҿ઼ PEP 㔃ਸˈഐ↔䘲ਸҾᔰਁሩ㥹⭈㟖㙀ਇⲴ䖜สഐ֌⢙Ǆੁٮᴤ❦ CP4 EPSPS ᱟ⭡ 455 њ≘ส䞨㓴ᡀⲴঅ䬮ཊ㛭ˈ⨶䇪࠶ᆀ䟿Ѫ 47.6 kDaǄᣇ 㥹⭈㟖֌⢙ѝⲴ CP4 EPSPS 䙊ᑨ൘ N-ᵛㄟᑖᴹа⇥ਦ㔯փ䖜䘀㛭ˈ⭘Ҿᕅሬ CP4 EPSPS ᇊսࡠਦ㔯փкˈ䘉ᱟ޵ⓀᙗἽ⢙ EPSPS 䞦൘㓶㜎޵Ⲵս㖞ǄӾᒿ ࡇкⴻˈCP4 EPSPS оἽ⢙ᶕⓀⲴ EPSPS ާᴹ 25-30%Ⲵ⴨լᙗˈо㓶㧼ᶕⓀⲴ EPSPS ާᴹ 50-90%Ⲵ⴨լᙗǄ㲭❦൘㳻ⲭ䍘а㓗㔃ᶴк਴㊫ EPSPS Ⲵ≘ส䞨ᒿ ࡇᒦнॱ࠶⴨լˈնⴞࡽᐢ⸕ⲴᡰᴹᶕⓀ EPSPS 䞦˄वᤜ CP4 EPSPS˅൘й㓗 ᤃᢁ㔃ᶴк儈ᓖа㠤˄Hammond, et al. 2013˅ǄӾᇊѹкᶕ䈤ˈ਼Ⓚ㳻ⲭᴹ⴨լ ⲴҼ㓗㔃ᶴ઼й㓗ᶴ䊑ˈӾ㘼ާᴹ⴨լⲴ࣏㜭˄Pearson, 2000˅Ǆ CP4 EPSPS ԕঅփᖒᔿᢗ㹼ۜॆ࣏㜭ˈн䴰㾱䖵䞦ᡆ⢩↺䠁኎⿫ᆀ䖵ࣙǄ Ѫᵚоᓅ⢙㔃ਸⲴ CP4 EPSPSחCP4 EPSPS Ⲵй㓗Ღփ㔃ᶴྲമ 2 ᡰ⽪ˈA മᐖ Ѫоᓅ⢙ S3P 㔃ਸਾⲴ䰝ਸ⣦ᘱⲴ䞦Ǆоަᆳח䞦ˈԕᔰ᭮⣦ᘱⲴᶴ䊑ᆈ൘˗ਣ EPSPS аṧˈCP4 EPSPS 䞦⭡є䜘࠶ॺ⨳⣦㔃ᶴฏ䙊䗷䬠䬮४䘎᧕ˈ⇿њॺ⨳ 㔃ᶴฏ൷⭡ཊњᒣ㹼Ⲵ-㷪᯻㓴ᡀˈ䞦ۜॆ⍫ᙗս⛩սҾєњॺ⨳⣦㔃ᶴฏѻ⣦ ⢙䰤ˈ⍫ᙗս⛩䱴䘁ਜ਼ᴹ䖳ཊᑖ⭥㦧Ⲵ≘ส䞨↻สˈׯҾоᓅ⢙⴨ӂ֌⭘Ǆоᓅ S3P Ⲵ㔃ਸ䈡ሬ䞦Ⲵᶴ䊑ਁ⭏ࢗ⛸ਈॆˈ৏ᵜ੸Āᢃᔰā⣦ᘱⲴєњॺ⨳⣦㔃ᶴ ฏ䰝ਸˈׯҾ䞦઼ PEP Ⲵ㔃ਸ˄ྲ B മᡰ⽪˅ǄCP4 EPSPS ሩ㥹⭈㟖Ⲵ㙀ਇ↓ᱟ ⭡Ҿަ⢩↺Ⲵࠐօᆖᶴ䙐⢩⛩˄Funke, et al. 2006˅ǄA മѝ₉㢢⧟⣦㔃ᶴᱟ䶐䘁 ᆸ↻ส Glu-354؍ᴹⲴ儈ᓖޡC-ᵛㄟⲴㅜ 347-358 ս↻สˈवᤜ EPSPS 䞦ᇦ᯿ᡰ ઼ Arg-357ˈо PEP/㥹⭈㟖Ⲵ㔃ਸᴹޣǄ⢩࡛䟽㾱Ⲵᱟ CP4 EPSPS ㅜ 100 սк 䬮䱫→Ҷ㥹⭈㟖о䞦Ⲵ㔃חⲴщ≘䞨↻ส˄㿱 B മѝ A100˅Ǆщ≘䞨кⲴ⭢ส ਸ˄Funke, et al. 2006˅ǄӪѪሶㅜ 100 սкⲴщ≘䞨ケਈᡀ⭈≘䞨ਾˈケਈⲴ CP4 EPSPS 䞦ሩ㥹⭈㟖ਈᗇ᭿ᝏǄѻࡽҏᴹ⹄ウ㺘᰾ˈབྷ㛐ᵶ㧼 EPSPS ѝ⴨ᓄ ս⛩ྲ᷌Ӿ⭈≘䞨ケਈᡀщ≘䞨˄Gly96Ala˅ˈ㜭ཏབྷབྷ䱽վަሩ㥹⭈㟖Ⲵ᭿ᝏ ᙗˈն਼ᰦо PEP ⲴӢ઼ᙗҏབྷབྷл䱽˄Barry et al. 1992; Padgette et al. 1996˅Ǆ ᤱ؍ CP4 EPSPS 䞦Ⲵ⤜⢩ᶴ䙐֯ᗇަሩ㥹⭈㟖н᭿ᝏˈն਼ᰦሩཙ❦ᓅ⢙ PEP ᖸ儈ⲴӢ઼ᙗǄ

16 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ 2. CP4 EPSPS ⓗ୕㔤㔃ᯊ♧ពമ

ѪоᓅחѪᵚоᓅ⢙㔃ਸⲴ CP4 EPSPS 䞦ˈԕᔰ᭮⣦ᘱⲴᶴ䊑ᆈ൘˗ਣחമᐖ ⢙ S3P 㔃ਸਾˈ䞦Ⲵᶴ䊑ਁ⭏ҶਈॆˈᡀѪ䰝ਸ⣦ᘱǄമѝ⧟⣦㔃ᶴᱟ䶐䘁 C-ᵛㄟ Ⲵㅜ 347-358 ս↻สˈ൘ᔰ᭮ᶴ䊑ᰦⲴ㠚⭡ᓖ䶎ᑨ儈ˈն൘䰝ਸ⣦ᘱлਈᗇᴹᒿॆˈ о䱴䘁 N-ᵛㄟⲴ≘ส䞨↻ส⴨ӂ֌⭘ˈޡ਼㓴ᡀ⍫ᙗս⛩Ǆ䘉њ⧟кਜ਼ᴹ EPSPS Ǆ⹄ޣᆸ↻ส Glu-354 ઼ Arg-357ˈо PEP/㥹⭈㟖Ⲵ㔃ਸᴹ؍ᴹⲴ儈ᓖޡ䞦ᇦ᯿ᡰ .䘋ަо PEP Ⲵ㔃ਸ˄Funke, et al׳ウ㺘᰾ˈঅԧ䱣⿫ᆀਟ㜭Պᖡ૽↔⧟Ⲵᶴ䊑ᒦ 2006˅Ǆ EPSPS 䞦˄वᤜ CP4 EPSPS˅ާᴹ儈ᓖⲴᓅ⢙⢩ᔲᙗǄⴞࡽᐢ⸕ሩ EPSPS ਈॆǄ׳䞦ާᴹ⭏⢙ᆖ᜿ѹⲴᓅ⢙ӵѪ S3P ઼ PEPǄ㥹⭈㟖н㜭ਁ⭏ EPSPS 䞦㧭㥹䞨оབྷ㛐ᵶ㧼ᶕⓀ EPSPS 䞦Ⲵ㔃ਸ㜭࣋䶎ᑨᕡˈо S3P ⴨∄ˈ㧭㥹䞨䴰㾱

17 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˈ䶎ᑨ儈⎃ᓖ˄mM 㓗˅᡽ᴹਟ㜭㻛 EPSPS 䞦ۜॆ˄Gruys, et al. 1992˅Ǆ⹄ウ㺘᰾ Ἵ⢙փ޵ཙ❦ᆈ൘ S3P ㊫լ⢙ྲ⭢ส㧭㥹䞨ǃཾቬ䞨ǃҼ≒㧭㥹䞨䜭нᱟ EPSPS Ⲵᓅ⢙˄Franz, et al. 1997˅Ǆ ٬ǃ⑙ᓖ઼⿫ᆀᕪᓖㅹഐ㍐ᖡ૽Ǆ CP4 EPSPS 㳻ⲭⲴ䞦⍫ᙗ઼っᇊᙗਇ pH ٬䗷儈ᡆ䗷վ䜭Պ䱽վ䞦⍫ᙗˈpH ሿҾ 5.5 ᰦ ᴰ䘲䞦⍫ pH Ѫ 9-9.5ˈpH CP4 EPSPS 䞦⍫ࠐѾᆼޘїཡˈн䗷⭡ pH ᕅ䎧Ⲵ䞦⍫ਈॆᒦ䶎нਟ䘶˄Padgette et al. 1993˅ǄCP4 EPSPS ሩ✝нっᇊˈ⑙ᓖ䎵䗷 75°C ᰦˈCP4 EPSPS Ⲵ࣏㜭⍫ ᙗণ䱽㠣Ự⍻䲀ԕлˈն࣐✝ᒦнᖡ૽㳻ⲭа㓗㔃ᶴⲴᆼᮤᙗ˄䈖㿱ᵜ⭣䈧Җѝ ㅜ 3.3 䜘࠶˅Ǆ䘉ㅖਸ✝ࣘ࣋ᆖ⌅ࡉˈণ⑙ᓖॷ儈Պ֯㳻ⲭ儈㓗㔃ᶴਈᙗˈᕅ䎧䞦⍫ᙗїཡǄCP4 EPSPS ሩ✝нっᇊⲴ⢩ᙗ㺘᰾ˈ൘ᐕъ࣐ᐕᶑԦлˈᴰ㓸Ⲵ䖜 สഐ⦹㊣ӗ૱ѝнཚਟ㜭ᆈ൘ާᴹ࣏㜭⍫ᙗⲴ CP4 EPSPS 㳻ⲭ˄Hammond and Jez 2011˅ǄоᡰᴹިරⲴ㳻ⲭ䞦㊫аṧˈCP4 EPSPS Ⲵۜॆ⍫ᙗ䘈ਇ৽ᓄⓦ⏢ѝ ⿫ᆀᕪᓖⲴᖡ૽Ǆ↓ᑨⲴ䍞ᆈ㕃ߢ⏢˄1mMKCl˅ᶑԦлˈCP4 EPSPS Ⲵ⢩ᔲ ⍫ᙗᴰ儈㜭ཏ䗮ࡠ 5.5 U/mgǄCP4 EPSPS Ⲵ⢩ᔲ⍫ᙗ䲿⵰⿫ᆀᕪᓖ໎བྷ㘼ॷ儈ˈ 㠣 50 mM KCl ᰦˈ䞦⍫ᙗ㜭ཏ໎࣐ 2-2.6 ؽ˄Padgette et al. 1993˅Ǆ →㠚 1996 ᒤㅜаֻ䖜สഐᣇ㥹⭈㟖བྷ䉶 40-3-2 ୶ъॆԕᶕˈ䗴ӺѪ cp4 epsps สഐᐢ㓿㻛䖜ޕཊ⿽֌⢙ѝˈ൘ޘ⨳㤳ത޵㻛୶ъॆ⿽Ἵᒦབྷ䟿⎸䍩ˈ वᤜབྷ䉶ǃ⦹㊣ǃ⋩㨌ǃỹ㣡ǃ㤌㬯ǃ⭌㨌ㅹㅹˈ䘉Ӌ䖜สഐ֌⢙ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭާᴹᶱ儈Ⲵ⴨լᙗǄབྷ䟿Ⲵᆹޘ䇴ԧ䍴ᯉ㺘᰾ˈ䲔Ҷሩ㥹⭈㟖Ⲵ ᣇᙗѻཆˈᣇ㥹⭈㟖䖜สഐ֌⢙൘ӗ䟿ǃॆᆖᡀ࠶઼㩕ޫᡀ࠶ᯩ䶒оਇփ૱⿽⴨ ∄⋑ᴹ᭩ਈ˄Delannay et al., 1995; Hammond et al., 1995; Harrison et al., 1996; Padgette et al., 1995; Nida et al., 1996˅Ǆ୶ъॆ䘁 20 ᒤᶕˈ䖜 cp4 epsps สഐⲴ ᣇ㥹⭈㟖㻛֌⢙Ӫ㊫઼ࣘ⢙ⴤ᧕ᡆ䰤᧕བྷ䟿⎸䍩ˈާᴹ䮯ᵏᆹޘᓄ⭘Ⲵ䇠ᖅǄ ཆⓀสഐ㺘䗮䈳᧗৺ MON 87427 Ⲵᔰਁᓄ⭘ ቭ㇑䙊ᑨ䇔Ѫ CaMV35S ੟ࣘᆀ኎Ҿ㓴ᡀර੟ࣘᆀˈնᴹӋ⹄ウ䇔Ѫ CaMV35S ੟ࣘᆀ൘н਼㓶㜎ᡆ㓴㓷ѝᡰ䈳᧗ⲴⴞⲴสഐ㺘䗮䟿ᱟнаṧⲴ ˄Benfey and Chua, 1989; Terada and Shimamoto, 1990; Williamson, et al., 1989; Yang and Christou, 1990˅ˈণާᴹ㓴㓷⢩ᔲᙗ䈳᧗֌⭘Ǆ䟷⭘ CaMV35S ੟ࣘᆀ䈳 ᧗Ⲵᣕ੺สഐ൘аӋἽ⢙Ⲵ㣡㊹ѝⲴ㺘䗮䟿䖳վ˄Sunilkumar, et al., 2002; Wilkinson, et al., 1997˅Ǆ MON 87427 ѝ cp4 epsps สഐ⭡໎ᕪරⲴ 35S˄e35S˅੟ࣘᆀ䈳᧗Ǆ㣡ὠ㨌㣡ਦ⯵∂ 35S สഐⲴ੟ࣘᆀ˄CaMV35S˅वᤜ㔃ᶴฏ A˄Domain A˅઼㔃ᶴฏ B ˄Domain B˅є䜘࠶˄മ 3˅ˈަѝ㔃ᶴฏ B ਟԕ໎ᕪⴞḷสഐⲴ㺘䗮Ǆe35S ੟ ࣘᆀᱟ൘ CaMV35S ੟ࣘᆀⲴส⹰к␫࣐Ҷањ㔃ᶴฏ B˄Benfey, et al., 1989˅Ǆ ഐ↔ˈe35S ੟ࣘᆀо CaMV35S ֌⭘ᯩᔿ⴨਼ˈਚᱟ࣏㜭䘋а↕໎ᕪ˄McPherson and Kay, 1994˅Ǆ MON 87427 䖜ॆ㺘䗮䖭փⲴ T-DNA ४ฏ޵वਜ਼Ҷањ cp4 epsps สഐ㺘䗮 ⴂˈ䈕สഐ㺘䗮ⴂᶴᔪᴹ e35S ੟ࣘᆀǃ⦹㊣ hsp70 สഐⲴㅜ 1 њ޵ਜ਼ᆀǃcp4 epsps สഐԕ৺ nos 㓸→ᆀǄޣҾ޵ਜ਼ᆀⲴ֌⭘ᵪࡦⴞࡽ䘈нॱ࠶␵ᾊˈնਟԕ㛟ᇊⲴ

18 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᱟ޵ਜ਼ᆀՊ䙊䗷࢚᧕ᵪࡦ৲оἽ⢙Ⲵสഐ㺘䗮䈳᧗˄䉒ṁㄐㅹˈ2011˅ˈᒦф亪 ᔿ֌⭘ݳԦ˄cis acting element˅Պ᧗ࡦ㣡㊹⢩ᔲᙗ㺘䗮˄Eyal, et al., 1995˅Ǆ MON 87427 ѝⲴ⢩ᇊ੟ࣘᆀ઼޵ਜ਼ᆀ㓴ਸ˄e35S-hsp70˅ਟԕᖸྭൠ䈳᧗ cp4 epsps สഐ൘н਼㓴㓷ѝⲴ㺘䗮ˈণ൘⦹㊣ṩǃ㤾ǃਦǃ䳼ᙗಘᇈ˄㣡эǃ ᷌ょ઼㊭㋂˅ѝ㺘䗮ˈ൘䳴ᙗಘᇈ˄㣡㊹ሿᆒᆀ઼∑㔂ቲ˅н㺘䗮ᡆ㺘䗮䟿ᖸվǄ

മ3. CaMV 35S઼e35S੟ࣘᆀࡍ㓗㔃ᶴ⽪᜿മ

19 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ӄǃ䖜สഐἽ⢙Ⲵᆹޘᙗ䇴ԧ 1. ਍։ὃ⢟Ⲻᆿޞᙝ䇺ԭ 1.1 ਍։ὃ⢟Ⲻ㜂Ქ䍺ᯏ ੃ૂެᆹ੃〦؍ᆜ੃θ 1.1.1 ਇփἽ⢙Ѫ⦹㊣ˈᆖ਽Ѫ Zea mays L.ˈ؇਽Ѫ⦹㊣ˈҏ〠֌⦹㴰哽ǃ㤎㊣ǃ ἂᆀㅹǄ 1.1.2 ࠼㊱ᆜ൦փ ṭษ⦹㊣൘࠶㊫к኎ҾঅᆀਦἽ⢙㓢ǃ⿮ᵜ、˄Gramineae˅ǃ⦹㴰哽᯿ ˄Maydeae˅ǃ⦹㴰哽኎˄Zea L.˅ǃ⦹㊣⿽˄Zea mays L˅ǃṭษ⦹㊣ӊ⿽˄mays˅Ǆ ൘ᰙᵏⲴ࠶㊫㌫㔏ѝˈ⦹㴰哽኎ӵᴹṭษ⦹㊣˄Zea mays L.˅ањ⿽ǄWilkes ˄1967˅ሶ⦹㴰哽኎˄བྷ࠽㥹ĀTeosinteā˅ᖂޕ⦹㴰哽኎ˈᡀѪަѝⲴањӊ ኎ˈवᤜєњ⿽˖аᒤ⭏໘㾯କ⦹㊣˄Zea mexicana˅઼ཊᒤ⭏⦹㊣˄Zea perennis˅Ǆ ṩᦞᯠⲴ⹄ウ㔃᷌ˈDoebley 亶ሬⲴ⹄ウሿ㓴ሩ⦹㴰哽኎޵਴࠶㊫অսⲴൠս 䘋㹼䟽ᯠࡂ࠶ˈࡦᇊҶᯠⲴ࠶㊫㌫㔏˄Doebley and Iltis, 1980˅˖

Family - Gramineae˄⿮ᵜ、˅ Subfamily - Panicoideae˄哽ӊ、˅ Tribe - Maydeae˄⦹㴰哽᯿˅ Genus - Zea˄⦹㴰哽኎˅ Species - Zea mays L.˄⦹㊣⿽˅ subsp. mays˄ṭษ⦹㊣ӊ⿽˅

⦹㊣৺ަ䘁㕈⢙⿽઼࠶㊫ᆖ࠶㊫ˈ䈧䈖㿱㺘 3Ǆ 1.1.3 䈋僂⭞਍։ὃ⢟૷〃δᡌ૷㌱ε੃〦 MON 87427 Ⲵ䖜ॆਇփᱟ LH198 × HiII ᵲӔ⿽ǄLH198 ާᴹ㢟ྭⲴߌ㢪ᙗ ḃ∄䖳᭿ᝏˈᒦфᇩ᱃޽⭏Ǆץ㘼 HiII ሩߌᵶ㧼˗⣦ LH198 ᱟ⭡սҾ⡡㦧ॾᐎⲴ Holden’s ৏⿽ޜਨҾ 1992 ᒤษ㛢Ⲵ㠚Ӕ㌫ˈ ᶕ㠚ᵲӔ㓴ਸ˄LH132 × B84˅× LH132ǄLH132 ҏᱟ Holden’s ৏⿽ޜਨⲴษ㛢 Ⲵ㠚Ӕ㌫ˈ㘼 B84 ࡉᱟ⡡㦧ॾᐎ・བྷᆖษ㛢Ⲵ㠚Ӕ㌫Ǆ HiII 㠚Ӕ㌫ᱟ䶎ᑨྭⲴ䖜ॆਇփˈᒦфޜՇਟԕᖸᇩ᱃ӾĀ⦹㊣䚇Ր䍴Ⓚ ৏৏⿽ѝᗳā㧧ᗇ˄Maize GDB, 2010˅ǄHiII ᶕ㠚ᵲӔ㓴ਸѪ B73 × A188 ˄Armstrong et al., 1991˅Ǆ 1.1.4 ᱥ䠄⭕〃䘎ᱥṳฯ〃 MON 87427 Ⲵਇփ LH198 × HiII ৺ަᶕⓀ㠚Ӕ㌫൷Ѫṭษ⿽Ǆ

20 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘 3. ṭษ⦹㊣࠶㊫৺ަ䘁㕈⢙⿽ 、 – ⿮ᵜ、 Gramineae ӊ、 – 哽ӊ、 Panicoideae ᯿ – ⦹㴰哽᯿Maydae 㾯ॺ⨳˖ I. ኎ - ⦹㴰哽኎ Zea A. ӊ኎ – 㑱㤲ӊ኎ Luxuriantes 1. 㑱㤲⦹㊣⿽ Zea luxurians˄2n = 20˅ 2. ཊᒤ⭏㊫⦹㊣⿽ Zea perennis˄2n = 40˅ ˅Ҽؽփཊᒤ⭏㊫⦹㊣⿽ Zea diploperennis˄2n = 20 .3 B. ӊ኎ - ⦹㴰哽ӊ኎ Zea 1. ṭษ⦹㊣⿽ Zea mays˄2n = 20˅ 1. ṭษ⦹㊣ӊ⿽ Z. mays mays˄2n = 20˅ 2. ሿ仆⦹㊣ӊ⿽ Z. mays parviglumis˄2n = 20˅ 3. ငင⢩ইᠸ⦹㊣ӊ⿽ Z. mays huehuetenangensis˄2n = 20˅ 4. ໘㾯କ⦹㊣ӊ⿽Z. mays mexicana˄Schrad.˅˄2n = 20˅ II. ኎ – ᪙ᬖ⿮኎ Tripsacum A. ӊ኎ – Tripsacum B. ӊ኎ – Fasciculata ⿽⿽ 1. T. andersomii˄2n = 64˅ 1. T. jalapense˄2n=72˅ 2. T. australe˄2n = 36˅ 2. T. lanceolatum˄2n =72˅ ਈ⿽ 3. T. fasciculatum˄2n = 36˅ a˅T. australe var. australe 4. T. maizar˄2n = 36, 72˅ b˅T. australe var. hirstum 5. T. pilosum˄2n = 72˅ 3. T. bravum˄2n = 36, 72˅ਈ⿽ 4. T. cundinamarce˄2n = 36˅ a˅T. pilosum var. guatemalense 5. T. dactyloides˄2n = 72˅ b˅T. pilosum var. pilosum ਈ⿽ a˅T. dactyloides var. hispidum b˅T. dactyloides var. dactyloides c˅T. dactyloides var. meridonale d˅T. dactyloides var. mexicanum 6. T. floridanum˄2n = 36˅ 7. T. intermedium˄2n = 72 8. T. manisuroides˄2n = 72˅ 9. T. latifolium ˄2n = 36˅ 10. T. percuvianum˄2n = 72, 90, 108˅ 11. T. zopilotense˄2n = 36, 72˅ ӊ⍢˖ I. ኎ – ⍱㣿᷌኎ Chionachne˄2n = 20˅ ⺜Ⳟ᷌኎ Schlerachne˄2n = 20˅ 㮿㤑኎ Coix ˄2n = 10, 20˅ й㻲᷌኎ Trilobachne˄2n = 20˅ ཊ㼄哽኎ Polytoca˄2n = 20˅ ᯿ – 儈㋡᯿ Andropogoneae I. ኎ - Manisuris

21 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 1.1.5 ৕ӝ൦਀ᕋ䘑ᰬ䰪 བྷ䟿⹄ウ㺘᰾ˈ⦹㊣ᖸਟ㜭Ҿ 7,000 ࡠ 10,000ᒤࡽ൘໘㾯କই䜘ᔰ࿻傟ॆǄ 㲭❦⦹㊣ޜ䇔Ⲵ䎧Ⓚⴞࡽ䘈⋑ᴹ᢮ࡠˈնᱟ໘㾯କ⦹㴰哽ᖸਟ㜭൘⦹㊣Ⲵ䚇Ր 㛼Ჟѝ䎧Ҷ䟽㾱֌⭘Ǆ କՖᐳਁ⧠ᯠབྷ䱶ᰦˈӾᲪ࡙ࡠ࣐᤯བྷই䜘Ⲵᖃൠ᮷᰾⽮Պ↓൘⿽Ἵ⦹㊣Ǆ କՖᐳҾ 1492 ᒤᤷࠪ൘ਔᐤই䜘⋯⎧ൠ४ᴹ⦹㊣⿽Ἵˈᒦ൘䘄എ㾯⨝⢉ᰦᢺ⦹ ㊣ᕅޕҶ⅗⍢˄Goodman, 1988˅Ǆ⦹㊣ᕅޕ⅗⍢ԕਾнѵˈ⦹㊣Ր䙽Ҷц⭼к ਟԕ⿽Ἵ⦹㊣Ⲵᡰᴹൠ४Ǆ 䈤ǄٷሩҾ⦹㊣Ⲵ䎧Ⓚˈⴞࡽᴹഋњѫ㾱Ⲵ

1˅ ᶕ㠚Ҿ໘㾯କ⦹㴰哽˄৸〠㊫⦹㊣ᡆབྷ࠽㥹˅˖࡙⭘໘㾯କ⦹㴰哽䘹㛢 ӗ⭏Ⲵ⦹㊣˗

䈤˖a˅⦹㊣ᶕ㠚Ҿ᷌㦊⦹㊣˗b˅࡙⭘⦹㊣઼й೺㥹ᵲӔ㹽⭏Ⲵٷй䟽 ˅2 ໘㾯କ⦹㴰哽˗c˅䙊䗷⦹㊣਼໘㾯କ⦹㴰哽ᡆ㘵й೺㥹⿽䰤ᵲӔˈᡆ 㘵਼Ҽ㘵਼ᰦ⿽䰤ᵲӔˈ䘋ॆࠪᶕⲴ⧠ԓ⦹㊣૱⿽˄Mangelsdorf, 1974˅˗

䈤˖⦹㊣ǃ໘㾯କ⦹㴰哽઼й೺㥹⤜・ൠᶕⓀҾ਼а⿽ᑨٷᲞ䙊䎧Ⓚ ˅3 ˗㿱նᵚ⸕Ⲵ⾆ݸ

䈤˖໘㾯କ⦹㴰哽⭡Ҿਾᶕ䚇Րᴹᙗケਈˈሬ㠤䳼ٷ⚮䳮ᙗᴹᙗケਈ ˅4 ょⲴਁ㛢ˈӾ㘼ӗ⭏Ҷ⧠ԓ⦹㊣Ǆ

↔ཆ䘈ᴹаӋ᧘ᯝˈྲ Andropogoneae ᯿䟼Ⲵ Coix ઼ Manisuris ኎Ⲵ䚇Ր㓴 ᙗǃ਼ޡ䈤ᐢ㓿䙊䗷⹄ウᵲӔ⿽Ⲵสഐ㓴ٷᡀҏሩ⦹㊣สഐ㓴ᴹаᇊ䍑⥞Ǆ䘉Ӌ ਟ㛢ᙗǃਈᔲᙗ઼⭏ᘱᆖἽ⢙⢩ᙗⲴ࠶⿫䘋㹼ҶỰ傼ˈҏ䙊䗷㘳ਔᆖ䇱ᦞ઼䙊䗷 ࠶ᆀ䚇Րḷ䇠Ⲵ֯⭘䘋㹼ҶỰ傼Ǆ 䈤䜭ᴹ䇨ཊн਼Ⲵ⹄ウᣕ䚃᭟ᤱǄնᱟˈབྷཊᮠⲴ䇱ᦞ᭟ᤱ⦹㊣ᶕ㠚ٷ䘉Ӌ Ҿ໘㾯କ⦹㴰哽˄Galinat, 1988˅Ǆ໘㾯କ⦹㴰哽Ⲵสഐ㓴㊫լҾ⦹㊣ˈ᱃Ҿ਼⦹ ㊣ᵲӔˈ䘈ާᴹࠐњ㊫լҾ⦹㊣ⲴἽ⢙⭏ᘱᆖ⢩ᙗˈ䘉Ӌ䇱ᦞ䇱᰾⦹㊣ᶱᴹਟ㜭䎧ⓀҾ໘㾯କ⦹㴰哽Ǆ ݳ 1511 ᒤᆹᗭⴱǉ亽ᐎᘇǊ⢙ӗѝᡰࡇޜѝഭቊᰐᇊ䇪Ǆޕ㊣օᰦ㻛ᕅ⦹ ⧽⨐〛㻛䇔Ѫᱟᡁഭ⦹㊣Ⲵᴰᰙ䇠䖭˄ѝഭ֌⢙䚇Ր䍴Ⓚˈ1994˅Ǆኡьߌ、䲒 ѫ㕆Ⲵǉѝഭ⦹㊣ṭษᆖǊ˄1986 ᒤ˅ѝ䇔Ѫǉ亽ᐎᘇǊѝⲴ⧽⨐〛ᓄ኎儈㋡Ⲵ ањ૱⿽Ǆᡁഭᴰᰙ䇠䖭⦹㊣Ⲵਔ㉽ᴹ 1555 ᒤ⋣ইǉᐙ৯ᘇǊǃ1551 ᒤ˄᰾హ 䶆 30 ᒤ˅⋣ইǉ㽴෾৯ᘇǊǃ1555 ᒤ⋣ইǉᐙ৯ᘇǊǃ1560 ᒤ⭈㚳ǉᒣ߹ᓌᘇǊǃ 1563 ᒤǉབྷ⨶ᓌᘇǊ઼ 1574 ᒤⲴǉӁই䙊ᘇǊㅹǄа㡜䇔Ѫ⦹㊣ᱟ⭡䱯᣹՟Ӫ Ӿ㾯⨝⢉ᑖࡠ哖࣐ˈ⭡哖࣐㓿ѝӊ㾯ӊՐޕᡁഭⲴ㾯े䜘ˈ޽Րࡠ޵䱶਴ⴱ˗ᡆ Ӿ哖࣐Րޕঠᓖ઼ᡁഭ㾯ই䜘ˈ㘼ਾੁަԆൠ४Ր᫝Ǆնᱟ⭡Ҿᖃᰦ⋯⎧ൠ४୶ ъਁኅ䗵䙏઼㡚⎧һъॱ࠶ਁ䗮ˈ⦹㊣㓿⭡⎧䐟Րޕьই⋯⎧ൠ४ҏᱟਟ㜭ⲴǄ ᰙᵏᕅ䘋Ⲵ⦹㊣ᱟ⺜㋂රⲴˈ傜喯ර⦹㊣ᱟ൘ 20 ц㓚 20 ᒤԓԕਾᕅޕⲴǄ൘

22 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 1760 ᒤԕࡽ⭡Ӂইᒯ㾯аᑖⲴ⺜㋂රൠᯩ૱⿽㓿ケਈ઼䘹ᤙᖒᡀҶ㌟䍘⦹㊣૱ ⿽˄䈖㿱ǉѝഭߌъਁኅਢǊ˄ѕз㤡ㅹˈ1992˅˗ǉѝഭߌъ、ᆖᢰᵟਢはǊ˄ằ ᇦࣹˈ1989˅˗ǉѝഭ֌⢙ṭษਢはǊ˄ୀ੟ᆷˈ1986˅Ǆ 1.1.6 ⭞䙊 ⦹㊣൘ 100 ཊњഭᇦᴹ୶ъॆ⿽ἽǄѫ㾱Ⲵ⦹㊣⭏ӗഭᱟ㖾ഭǃѝഭǃᐤ㾯ǃ ໘㾯କǃ⌅ഭ઼ঠᓖˈঐޘц⭼⦹㊣ᙫӗ䟿Ⲵ 75%Ǆ൘ӊ⍢ˈѝഭⲴ⦹㊣⭏ӗঐ ѫሬൠսǄѝഭᱟц⭼кӵ⅑Ҿ㖾ഭⲴㅜҼབྷ⦹㊣⭏ӗഭ˄Morris, 1998˅ˈ⿽Ἵ ⦹㊣ѫ㾱ᱟѪҶ㧧ᗇ⦹㊣㊭㋂ˈަབྷཊᮠ⭘֌ࣘ⢙侢ᯉˈնᱟҏᴹ⴨ᖃབྷⲴ䜘࠶ ࡦᡀӗ૱⭘Ҿ伏૱ǃ㦟⢙઼ᐕъӗ૱ㅹᒯབྷ亶ฏǄӾ 2000 ᒤᔰ࿻ˈ൘⋣ইⴱ઼ ѝഭ㾯ेൠ४Ⲵ 3 њⴱ⦹㊣㻛⭘ᶕᨀ⛬⟳⭘䞂㋮Ǆᓄ⭘㤳ത⧠ᐢᢙབྷࡠьेǃ⋣ ेǃኡьǃ⊏㣿ㅹ䇨ཊൠ४Ǆ ⦹㊣㊭㋂ѝ㜊ң㓖ঐ 82%ǃ㜊㓖ঐ 12%ǃⳞ ቲ 㓖 ঐ 5%˄ަѝ 2.2%⭡㋇㓔㔤 ᶴᡀ˅ǃቆㄟ㓖ঐ 1%˄Earle et al., 1946; Perry, 1988˅ǄӪ㊫ሩ⦹㊣Ⲵ⎸䍩ᯩᔿѫ 㾱ᱟ࣐ᐕ伏૱ᡆ伏૱ᡀ࠶ˈਚᴹᖸቁⲴ∄ֻⴤ᧕伏⭘㊭㋂˄Watson, 1988˅Ǆ⭘Ҿ 伏૱઼ᐕъⴞⲴⲴ⦹㊣㊭㋂བྷཊᮠ˄77%˅䙊䗷⒯⼘㊹ᯩᔿ䘋㹼࣐ᐕǄ൘㊭㋂⒯ ⼘Ⲵ䗷〻ѝˈ㜊㣭ਟԕ㻛ᨀਆ⭘Ҿ⭏ӗ⦹㊣⋩ˈնᴤѫ㾱Ⲵӗ૱ᱟ⏰㊹Ǆ Ӫ㊫伏૱˖ ሶ㜊㣭䜘࠶਼㜊ң࠶⿫ˈ㓿঻Ῐਟ࠶⿫ࠪ⦹㊣⋩ˈਟ⭘Ҿࡦ䙐Ӫ䙐哴⋩ǃ✩ 䈳⋩઼✔⛨⋩ˈ׋Ӫ㊫伏⭘Ǆ⦹㊣⋩ቭ㇑ਚঐᮤњἽ⢙⋩ᐲ൪Ⲵаሿ䜘࠶ˈնަ ֌⭘Ǆ⦹㊣⋩൘ߧ㯿⑙ᓖлާᴹڕ؍ਜ਼ᴹⲴཊн価઼㜲㛚䞨তާᴹ䟽㾱Ⲵ㩕ޫ઼ 㢟ྭⲴ俉ણǃ㢢⌭ǃっᇊᙗ઼▴␵ᓖǄഐѪਜ਼ᴹӊ哫㜲㛚䞨઼㔤⭏㍐ Eˈ㻛䇔Ѫ ᱟа⿽儈㓗Ἵ⢙⋩Ǆ䘉⿽㋮ࡦ⋩ 50%ᐖਣ⭘Ҿ✩⛨⋩઼⋉᣹⋩ˈ25%⭘Ҿ⭏ӗӪ 䙐ྦ⋩ˈ25%⭘ҾަԆⴞⲴˈ঻ῘਾⲴ㜊㣭ਟԕ⭘֌侢ᯉǄ ሶ⦹㊣㜊ң⼘㊹ˈ❦ਾ⿫ᗳ༴⨶ˈਟԕሶ⏰㊹Ӿ⦹㊣⑓࠶⿫ࠪᶕǄ㓖 40% Ⲵ⏰㊹֌Ѫ伏૱ⴤ᧕⎸䍩ᡆ㘵⭘ҾަԆᐕъⴞⲴˈ㓖 60%䖜ॆѪ਴⿽⭌ણࡲ ˄White and Pollack, 1995˅Ǆ⏰㊹ਟԕ䖜ॆᡀཊ⿽⭌ણࡲ઼ਁ䞥ӗ૱ˈवᤜ儈᷌ ㌆⦹㊣㌆⍶઼҉䞷Ǆ⦹㊣⏰㊹ҏਟԕ֌Ѫа⿽伏૱㓴ᡀᡀ࠶ˈྲңࡦ૱઼ߠ◰߼ǃ 䶒㋺伏૱ǃ✔ࡦ伏૱ǃ⊔ᯉǃ䞡⋩઼⎃⊔ǃ⋉᣹ǃ㚹ǃ⿭઼劬ࡦ૱ǃ㌆઼᷌侞ᯉ ㅹㅹǄ ࣘ⢙侢ᯉ˖ ㊣㊭㋂䟼ਜ਼ᴹⲴԓ䉒㜭ᱟ⢢⮌侢ᯉ䟼֯⭘Ⲵ਴⿽䉧⢙ѝᴰ᱃Ҿԓ䉒Ⲵ㜭⦹ 䟿˄Ensminger et al., 1990˅Ǆ⦹㊣㊭㋂䟼ᴹབྷ㓖 83%Ⲵ⻣≤ॆਸ⢙ˈԕ⏰㊹ǃᠺ 㚊㌆ǃ㋺㋮ǃ㌆ǃ㓔㔤㍐઼ॺ㓔㔤㍐Ⲵᖒᔿᆈ൘Ǆ⻣≤ॆਸ⢙ѝ⏰㊹Ⲵਜ਼䟿ᴰ儈ˈ ⦹㜭ᨀ׋བྷ䜘࠶㜭䟿Ǆ㓔㔤वᤜ㓔㔤㍐઼ॺ㓔㔤㍐ˈਟԕ㻛৽࠽ࣘ⢙⎸ॆ੨᭦Ǆ ㊣㊭㋂ѝ⋩Ⲵਜ਼䟿བྷ㓖Ѫ 4%ˈ⦹㊣⋩ਜ਼ᴹབྷ䟿Ⲵ 18:2 ӊ哫䞨ˈ䘉ᱟ⥚઼ᇦ⿭ᗵ 䴰Ⲵཊ㚊н価઼㜲㛚䞨ѻаǄоަԆ䉧⢙㊭㋂⴨∄ˈ⦹㊣㊭㋂ѝ㳻ⲭ䍘ਜ਼䟿⴨ሩ ⦹䖳վˈ㓖ঐ㊭㋂ᒢ䟽Ⲵ 10%ˈնᱟ⭡Ҿ⦹㊣൘ࣘ⢙侢ᯉѝᡰঐ∄ֻ䖳儈ˈᡰԕ ㊣ᱟ侢ᯉѝḀӋᗵ䴰≘ส䞨ⲴᶕⓀˈྲ⦹㊣㊭㋂ᱟ⭢⺛≘䞨Ⲵ㢟ྭᶕⓀˈնнᱟ 23 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䎆≘䞨઼㢢≘䞨ⲴᶕⓀǄ⭢⺛≘䞨઼䎆≘䞨ᱟԕ⦹㊣Ѫѫ㾱侢ᯉᡀ࠶Ⲵᇦ⿭ǃ⥚ ઼ަԆ⢢⮌ѝєњᴰ䟽㾱Ⲵ䲀ࡦᙗ≘ส䞨˄NRC, 2001˅Ǆ ⦹㊣䶒ㅻ㊹ǃ⏰㊹⑓઼㫨便ᒢ䞂㌏ˈ֌Ѫ⒯⌅઼ᒢ⌅⼘㊹Ⲵ࢟ӗ૱ˈᱟ⢢⮌ 侢ᯉⲴ䟽㾱ᡀ࠶Ǆ⦹㊣⏰㊹⑓઼䶒ㅻ㊹ᱟ⒯⌅⼘㊹Ⲵ࢟ӗ૱ˈਟ᧪ޕࣘ⢙侢ᯉѝǄ 䶒ㅻ䟼㳻ⲭ䍘ਜ਼䟿䖳儈˄60%˅ˈᱟ㊫㜑㖇ঌ㍐Ⲵ䟽㾱ᶕⓀǄᆳ䙊ᑨ⭘Ҿ⢋ǃ劬ǃ ᇦ⿭ǃᇐ⢙઼ަԆࣘ⢙侢ᯉˈѫ㾱⭘൘ᇦ⿭Ⲵᰕ㋞䟼Ǆ⦹㊣⏰㊹⑓˄⒯Ⲵᡆ㘵ᒢ Ⲵ˅ᱟᶱྭⲴ侢ᯉˈަ⏰㊹઼⋩ਜ਼䟿վˈਟ⎸ॆ㓔㔤ਜ਼䟿儈ˈᱟ㳻ⲭ䍘˄20%˅ Ⲵ䟽㾱ᶕⓀˈѫ㾱⭘Ҿྦ⢋઼㚹⢋Ⲵᰕ㋞䟼Ǆ 䲔㊭㋂ཆˈ⦹㊣ҏਟԕࡦᡀ䶂䍞侢ᯉˈ⭘֌侢ޫ൪઼ྦ⢋൪侢லǄ 1.1.7 ൞ള޻Ⲻᓊ⭞᛻߫ ⦹㊣ᰙ൘ 16 ц㓚ቡ⭡⅗⍢Րᮉ༛ᕅ䘋ѝഭˈॱгц㓚Ր䙽Ҷѝഭ਴ൠǄⴞ ࡽˈ⦹㊣൘ѝഭⲴ䇨ཊⴱԭ䜭ᴹ⿽Ἵ˄Rozelle and Carter, 2001˅Ǆ ⦹㊣൘ѝഭⲴߌъ⭏ӗ㔃ᶴѝ䎧⵰䟽㾱Ⲵ֌⭘ˈᱟՈ㢟Ⲵ侢ᯉǃ䟽㾱ⲴⲴᐕ ъ৏ᯉ઼Ո䍘Ⲵ㋞伏֌⢙Ǆѝഭᱟц⭼кѫ㾱⦹㊣⭏ӗഭѻаǄ㖾ഭߌъ䜘㔏䇑 ᮠᦞ㺘᰾ˈ2012 ᒤѝഭⲴ⦹㊣ӗ䟿Ѫ 2.08 ӯ੘ˈӵ⅑Ҿ㖾ഭ˄2.74 ӯ੘ᱟޘ⨳ ㅜҼབྷ⦹㊣⭏ӗഭ˄USDA-NASS, 2013˅Ǆ⦹㊣࠶ᐳ൘े㓜 50°̚20°䰤㠚ьे㠣㾯ইањ⤝䮯Ⲵൠᑖˈवᤜ 14 њⴱǃᐲǃ㠚⋫४Ǆѝഭ⦹㊣ᑖਟ࠶Ѫ 6 њӗ४˖ I. ेᯩ᱕᫝⦹㊣४˗II. 哴␞⎧༿᫝⦹㊣४˗III. 㾯ইኡൠ⦹㊣४˗IV. ইᯩш䲥 ⦹㊣४˗V. 㾯े♼Ⓓ⦹㊣४઼ VI. 䶂㯿儈৏⦹㊣४˄䈖㿱 1.3.1 䜘࠶઼മ 4˅Ǆ ަѝˈࡽ 3 њӗ४ঐ⦹㊣᫝⿽ᙫ䶒〟Ⲵ 80%Ǆ শਢкˈ⦹㊣অ⤜ᡆоަԆ䉧㊫㋞伏а䎧ࡦ֌਴⿽ѝഭӪௌ伏Ⲵ伏૱˄ྲ⦹ ㊣侬ǃ䶒वǃ㳻㌅ㅹ˅Ǆ勌⦹㊣˄वᤜᲞ䙊⦹㊣઼⭌㌟⦹㊣˅ਟⴤ᧕伏⭘Ǆ൘䗷 ৫Ⲵ 20 ᒤ䰤ˈ⦹㊣ᡀѪᴰ䟽㾱Ⲵ侢ᯉ֌⢙ѻаǄ⦹㊣֌Ѫ৏ᶀᯉਟ⭏ӗ 300 ཊ ⿽ᐕъӗ૱ᡆ֌Ѫࡦ㦟৏ᯉǄ⦹㊣Ⲵᴰ㓸⭘䙄ҏ൘䗵䙏ਈॆ˗⦹㊣↓䎺ᶕ䎺ཊൠ ⭘Ҿབྷරޫ⥚൪઼ᇦ⿭൪ˈ㘼н޽ӵӵᱟа⿽㋞伏֌⢙ᡆ㘵ߌᇦ䲒ޫ⥚ޫ呑Ⲵ侢 ᯉ˄Rozelle and Carter, 2001˅Ǆѝഭབྷ㓖 75%Ⲵ⦹㊣⭘Ҿࣘ⢙侢ᯉˈަ։Ⲵ⭘Ҿ Ӫ㊫⎸䍩઼ᐕъ⭘䙄Ǆᦞ㔏䇑˄㘏ࠔ㤡઼ᶘᲃݹ, 2006˅ˈѝഭት≁ᒣ൷⇿ḷ߶Ӫ 䟿Ѫ 9.9 ݻ˄㺘 4˅Ǆ80 ᒤԓ൘ѝഭⲴᐲ൪㓿⍾᭩䶙઼䈳ᮤޕཙ⦹㊣伏⢙Ⲵᩴ⇿ 䘋Ҷ⮌⢗ъⲴਁኅˈҏഐ↔ᨀ儈Ҷ⦹㊣֌Ѫ侢׳Tuan and Peng, 2001˅ᶱབྷൠ˄ ᯉⲴ䴰≲䟿˄Rozelle and Carter, 2001˅Ǆ 㺘4. ѝഭ෾ґት≁⦹㊣伏⢙ᩴޕ䟿

ൠ४ བྷ෾ᐲ ѝሿ෾ᐲ а㊫ߌᶁ Ҽ㊫ߌᶁ й㊫ߌᶁ ഋ㊫ߌᶁ ᒣ൷ ᩴޕ䟿˄g/Ӫ/d˅ 8.6 8.0 7.1 7.9 19.5 17.3 9.9

ᓭૂ⭕ᘷ⧥ູᱥੜਇ⭕䗽у࡟ᖧଃڛ㊱ሯӰ 1.1.8 ݳࡽ 2,700 ᒤቡ֌Ѫ㋞伏֌⢙䘋㹼⿽ἽҶޜ㊣৏ӗҾ໘㾯କˈᰙ൘⦹ ˄Salvador, 1997˅ǄࠐⲮᒤᶕˈаⴤᱟࣘ⢙侢ᯉ઼Ӫ㊫Ⲵѫ伏Ǆ⦹㊣㊭㋂৺ަ࣐ ᐕᡀ࠶ਟԕ⭘൘਴⿽伏૱઼ࣘ⢙侢ᯉӗ૱䟼Ǆ伏⭘⦹㊣ᰦਁ⭏Ⲵ䗷᭿৽ᓄᶱѪቁ 24 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㿱˗⹄ウᴰཊⲴᱟ਼㜲㊫Ր䙂㳻ⲭ䍘ᴹޣⲴ䈮仈˄Pastorello et al., 2000˅Ǆ↔ཆˈ ⦹㊣㊭㋂ѝнਜ਼∂㍐઼㠤᭿㳻ⲭ˄Watson, 1982; White and Pollak, 1995˅Ǆ ቭ㇑⦹㊣൘ޘц⭼ᒯ⌋⿽ἽˈնᱟӪԜᒦн䇔ѪᆳՊᡀѪ≨ѵᙗᵲ㥹ᡆ㘵䳮 Ҿ䱢⋫Ⲵᵲ㥹Ǆ↓ྲᡁԜӺཙᡰ⸕ˈ⭡Ҿ⦹㊣䳼ᙗ㣡ᒿ˄᷌ょ˅㜭䲀ࡦ⿽ᆀⲴՐ ᫝ˈഐ↔⦹㊣н㜭൘䟾⭏⧟ຳ䟼ᆈ⍫Ǆ 䗴ӺѪ→ˈ⿽Ἵ⦹㊣ǃ伏⭘⦹㊣˄वᤜ伏⭘ਜ਼ᴹ⦹㊣ᡀ࠶Ⲵ伏૱˅ǃ֌Ѫ侢 ᓧ৺⭏ᘱ⧟ຳӗ⭏䗷н࡙ᖡ૽Ⲵᣕڕᯉᡆ⭘Ҿᐕъ⭘䙄ˈ䜭ᵚ㿱ሩӪ㊫ᡆࣘ⢙Ⲵ 䚃Ǆ 1.1.9 Ԅ়ਨрⵁθ਍։ὃ⢟╊Җᡆᴿᇩὃ⢟δྸᵸ㥿ㅿεⲺਥ㜳ᙝ ⦹㊣ᱟ䎧ⓀҾ㾯ॺ⨳ѪᮠнཊⲴѫ㾱֌⢙⢙⿽ѻаǄ㲭❦ⴞࡽ䘈⋑ᴹ᢮ࡠ ޜ䇔Ⲵ⦹㊣䎧Ⓚˈնᶱᴹਟ㜭໘㾯କ⦹㴰哽൘⦹㊣Ⲵ䚇Ր㛼Ჟ䟼䎧Ҷ䟽㾱Ⲵ֌ ⭘Ǆ⦹㊣Ӿ䟾⭏⢙⿽傟ॆᡀ׍䶐Ӫ㊫㙅֌᡽㜭⭏ᆈⲴṭษ⢙⿽ˈ㓿শҶᖸ䮯Ⲵ ᰦ䰤Ǆ↓ྲᡁԜӺཙᡰ⸕ˈоᵲ㥹н਼ˈ⭡Ҿ⦹㊣䳼ᙗ㣡ᒿ˄᷌ょ˅㜭䲀ࡦ⿽ ǄੁٮᆀⲴՐ᫝ˈ⦹㊣н㜭൘䟾⭏⧟ຳ䟼ᆈ⍫ˈഐ↔ṭษ⦹㊣нާᴹᵲ㥹 1.1.10 ᱥੜᴿ䮵ᵕᆿޞᓊ⭞Ⲻ䇦ᖋ ݳࡽ 2700 ᒤቡ֌Ѫ㋞伏֌⢙䘋㹼⿽ޜࡽᡰ䘠ˈ⦹㊣৏ӗҾ໘㾯କˈᰙ൘ྲ ἽҶ˄Salvador, 1997˅Ǆ䮯ᵏԕᶕˈ⦹㊣аⴤᱟӪ㊫઼ࣘ⢙Ⲵѫ㾱伏⢙ᶕⓀˈ ⭘শਢǄ֯ޘᆳ⭘Ҿ伏૱ާᴹ䮯ᵏⲴᆹ 1.2 ਍։ὃ⢟Ⲻ⭕⢟ᆜ⢯ᙝ 1.2.1 жᒪ⭕䘎ᱥཐᒪ⭕ ⦹㊣ᱟ䳼䳴਼Ṛᔲ㣡Ⲵаᒤ⭏⿮ᵜ、ṭษἽ⢙ˈ䴰㾱Ӫᐕ䖵ࣙ᡽ਟՐ᫝⿽ ᆀԕᆼᡀ㑱⇆઼⭏ᆈ˄OECDˈ2003˅Ǆ 1.2.2 ሯӰ㊱਀ެᆹ⭕⢟ᱥੜᴿ∈θྸᴿ∈θᓊ䈪᱄∈ᙝᆎ൞Ⲻ䜞փ ਀ެ∈ᙝⲺᙝ䍞 ṩᦞ OECD˄2002˅ˈ⦹㊣䟼ᴹࠐњӪԜ⟏⸕Ⲵᣇ㩕ޫഐᆀˈवᤜἽ䞨ǃ бᐳ˄2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3 (4H)-one (DIMBOA)˅ǃỹ ᆀ㌆ǃ㜠㳻ⲭ䞦ᣁࡦࡲ઼㜠ࠍң㳻ⲭ䞦ᣁࡦࡲǄ ㊣㊭㋂ѝⲴἽ䞨㜭㷟ਸⸯ⢙㩕ޫݳ㍐ˈवᤜ䫉ǃ䭱ǃ䫮ǃ䫱઼䬼ˈ֯অ⦹ 㛳ࣘ⢙൘⭏⨶ᆖкн㜭⎸ॆ੨᭦䘉Ӌ㩕ޫݳ㍐˄Liener, 2000˅ǄӪԜ਼ᰦ䇔Ѫ Ἵ䞨ሩҾࣘ⢙ˈ⢩࡛ᱟ䶎৽࠽ࣘ⢙ˈᱟа⿽䟽㾱Ⲵᣇ㩕ޫഐᆀˈഐѪᆳ㜭䱽վ ⼧Ⲵ⭏⢙ᆖᴹ᭸ᙗǄഐ↔൘侢ᯉ࣐ᐕ䗷〻ѝӪԜ䙊ᑨՊੁ⥚઼ᇦ⿭侢ᯉѝ仍ཆ ␫࣐Ἵ䞨䞦ˈԕᨀ儈⼧Ⲵ࡙⭘⦷Ǆ бᐳ˄DIMBOA˅኎Ҿа㓴ԓ䉒⢙ˈণ≗㛏䞨઼䞊㊫ॆਸ⢙ˈ䙊ᑨਟԕ൘ 䉧㊫Ἵ⢙ѝਁ⧠ǄDIMBOA 䟼Ⲵ㪑㨴㌆㤧ণ˖DIMBOA-glcˈᱟ൘⦹㊣ᴰࡍਁ

25 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㛢䱦⇥ᆈ൘Ҿൠк䜘㔯㢢㓴㓷઼ṩ䜘㓴㓷䟼Ⲵ䘉㊫ॆਸ⢙ѝᴰ䟽㾱Ⲵа⿽ ˄Cambier et al., 2000˅ǄDIMBOA-glc ൘ਇՔⲴἽ⢙㓴㓷䟼ˈ࡙⭘䞦৫䲔㪑㨴㌆㤧ˈਈᡀ DIMBOAˈਾ㘵ሩᰶ㲛ᴹ∂ᙗ˄OECD, 2002˅Ǆ ỹᆀ㌆ᱟа⿽վ࠶ᆀ䟿Ⲵ⻣≤ॆਸ⢙ˈ⭡Ҿ伏⭘ਾᆳ㜭ӗ⭏≄փ䙐ᡀ㛐㛳 ≄㛰ˈӪԜ䇔Ѫᆳᱟа⿽ᣇ㩕ޫഐᆀ˄Maynard et al., 1979˅Ǆ ↔ཆˈ⦹㊣䟼䘈ᴹᖸቁ䟿Ⲵ㜠㳻ⲭ䞦઼㜠ࠍ㳻ⲭ䞦Ⲵᣁࡦഐᆀˈн䗷䙊ᑨ ӪԜ䇔Ѫ䘉Ҽњഐᆀ䜭нՊሩ㩕ޫӗ⭏䟽㾱ᖡ૽˄White and Pollak, 1995˅Ǆ 䲔Ҷ䘉Ӌᣇ㩕ޫഐᆀཆˈ⋑ᴹᣕ䚃㺘᰾⦹㊣ѝਜ਼ᴹަᆳᴹ∂⢙䍘Ǆ 1.2.3 ᱥੜᴿ㠪ᮅ৕θྸᴿθᓊ䈪᱄㠪ᮅ৕ᆎ൞Ⲻ䜞փ਀ެ㠪ᮅ⢯ᙝ ⦹㊣䮯ᵏ֌ѪӪ㊫઼ࣘ⢙Ⲵ伏⢙ˈ䙊ᑨ኎Ҿ䶎䗷᭿৏ᙗ伏⢙Ǆⴞࡽᖸቁᴹ ޣ伏⭘⦹㊣ӗ૱ਾਁ⭏䗷᭿৽ᓄⲴᣕ䚃˄OECD, 2002˅Ǆ䗷৫ॱࠐᒤѝˈ൘㖾 ᣕ䚃˄Pauls and Cross, 1998; Tanaka et al., 2001˅Ǆޣഭਚᴹєֻ⴨ Jones et al.˄1995˅䇔Ѫˈ䇨ཊሩ㊭㋂㺘⧠䗷᭿ⲴӪᇎ䱵кᱟሩ㣡㊹䗷᭿ˈ 䘉ӋӪѝᴹབྷ㓖 80%ⲴӪ䘋㹼伏⢙᭫ࠫ䈅傼ᰦ⋑ᴹӗ⭏Ѥᒺ䗷᭿⯷⣦Ǆ↔ཆˈ ൘ᴮ㓿ሩ㊭㋂ӗ⭏䗷᭿৽ᓄⲴӪѝˈ㲭❦ཊᮠՊሩሿ哖㳻ⲭ䍘ਁ⭏䗷᭿৽ᓄ ˄~75%˅ˈնሩ⦹㊣㊭㋂ӗ⭏䗷᭿৽ᓄⲴӪᶱቁˈаⲮཊս⯵Ӫѝнࡠ 6 սੁ 䈕ሿݯ、䗷᭿৽ᓄу、ѝᗳᣕ੺ਁ⭏Ҷ䗷᭿৽ᓄǄ ᶕ㠚Ҿ᜿བྷ࡙Ⲵєԭᣕ੺˄Pastorello et al., 2000; Pasini et al., 2002˅㺘᰾ˈ ᴹаӋ䗷᭿⯵Ӫࠪ⧠Ⲵ伏૱䗷᭿৽ᓄˈ਼伏⭘वᤜ⦹㊣㋕൘޵Ⲵ⦹㊣ӗ૱ࠪ⧠ Ⲵ䗷᭿৽ᓄ⯷⣦⴨а㠤ˈަѝᶕ㠚᤯⌒䟼Ⲵ 6 ս⯵Ӫ䖫ᓖ伏૱᭫ࠫ䈅傼㔃᷌੸ 䱣ᙗǄնᱟˈк䘠᜿བྷ࡙⹄ウѝᡰᣕ੺Ⲵ⯵Ӫˈ䙊ᑨሩᵲ㥹ǃprunidae ㊫Ἵ⢙ ӗ⭏Ⲵ㣡㊹ǃ㊭㋂৺俉ᯉㅹˈ䜭Պਁ⭏ཊ䟽䗷᭿৽ᓄǄཊ䟽䗷᭿৽ᓄ֯ᗇѤᒺ к䇶࡛⢩ᇊ⯵ֻⲴ㠤᭿৏ᰦާᴹн⺞ᇊᙗ˗⭡Ҿ䗷᭿৽ᓄѻ䰤ᡆཊᡆቁ▌൘Ⲵ Ӕ৹֌⭘ˈ֯ᗇⳞ䈅䇺ᯝ઼⿫փ IgE 㔃ਸ䈅傼ᴤѪ༽ᵲ˄Pastorello et al., 2000˅Ǆ ⦹㊣㊭㋂ѝѫ㾱㠤᭿৏ѻаѪ࠶ᆀ䟿 9kDaⲴ㜲䖜䘀㳻ⲭ˄LTPˈlipid transfer protein˅ˈ኎Ҿ伏⢙ѫ㾱䗷᭿৏ॱޛ䟋ӊᇦ᯿ˈо≤にǃṳᆀоབྷ哖ㅹ䗷᭿৏਼ Ⓚˈ⋑ᴹਁ⧠⦹㊣⢩ᔲⲴ LTP 䗷᭿৏˄Pastorello et al.ˈ2000˅Ǆ⦹㊣оަԆἽ ⢙㣡㊹䗷᭿৏⴨ޣᡀԭሩ∄࠶᷀⋑ᴹਁ⧠⦹㊣⢩ᔲⲴ㠤᭿৏˄Petersen A., 2006˅Ǆⴞࡽˈ䘈⋑ᴹ䏣ཏⲴ䇱ᦞᶕҶ䀓൘᜿བྷ࡙ᡆൠѝ⎧ൠ४⦹㊣ӗ૱䗷᭿৽ ᓄ⍱㹼Ⲵ⢩⛩ˈҏ䘈⋑ᴹᶕ㠚Ҿц⭼ަԆൠ४ޣҾ⦹㊣伏૱䗷᭿৽ᓄⲴᣕ੺ᇎ Ǆ䘉㺘᰾ˈ⭡Ҿ伏⭘⦹㊣৺ަ㹽⭏ӗ૱ˈሩӪ㊫䙐ᡀ䗷᭿৽ᓄ仾䲙Ⲵਟ㜭ᙗֻ ᖸվ˄Scibilia et al., 2008˅Ǆ 1.2.4 㑷⇌ᯯᕅᱥᴿᙝ㑷⇌䘎ᱥᰖᙝ㑷⇌θྸѰᴿᙝ㑷⇌θᱥ㠠㣧ᦾ ㊿䘎ᱥᔸ㣧ᦾ㊿ᡌᑮᔸ㣧ᦾ㊿χᱥ㲡჈Ֆ㊿䘎ᱥ伄჈Ֆ㊿ ⦹㊣ᱟᴹᙗ㑱⇆֌⢙ˈᱟ㠚ӔǃᵲӔ൷ӢਸⲴ⢙⿽˄Wilkes, 1972, 1989˅Ǆ 㣡㊹ⲴՐ᫝ᯩᔿѫ㾱ᱟ仾ჂՐ㊹˄OECD, 2003b˅ˈ㲛ჂՐ㊹䖳ቁǄ㠚㣡ᦸ㊹ аἽṚ޵䚇Ր⢩ᖱ䎻ੁ㓟ਸˈ㘼ᔲ㣡ᦸ㊹㜭㔃ਸн਼ἽṚⲴ䚇Ր⢩⛩ˈ䘉਼֯

26 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 а㠚ӔǃᵲӔⲴᾲᘥ઼⭡↔ӗ⭏Ⲵӗ䟿᭸ᓄᱟ⧠ԓ⦹㊣ᵲӔ㛢⿽Ⲵ⨶䇪ส⹰Ǆ ᴹ⭏ભ࣋Ⲵ㣡㊹ਟԕՐ᫝Ⲵ䐍⿫ਆߣҾ仾Ⲵ⢩⛩ǃ⒯ᓖ઼⑙ᓖㅹǄ 1.2.5 ൞㠠❬ᶗԬсфੂ〃ᡌ䘇㕎〃ⲺᔸӚ⦽ ⦹㊣ᱟ仾Ⴢᦸ㊹ǃ㠚ӔӢਸǃᵲӔਟ㛢Ⲵ⢙⿽˄Wilkes, 1972, 1989˅Ǆᴹ⭏ ભ࣋Ⲵ㣡㊹ਟԕՐ᫝Ⲵ䐍⿫ਆߣҾ仾Ⲵ⢩⛩ǃ⒯ᓖ઼⑙ᓖǄ䲔ҶḀӋ⠶㻲⦹㊣૱ ⿽઼ᵲӔ⿽ഐѪањ䝽ᆀփ䚇Րഐᆀ˄൘ḃ㢢փ 4 кⲴ GasǃGa ઼ ga ㅹսสഐ ㌫ࡇ˅ᖡ૽ަਇ㋮ԕཆˈ⦹㊣о䘁㕈⿽䰤ਟԕ䘋㹼ᵲӔᦸ㊹Ǆ ⦹㊣઼໘㾯କ⦹㴰哽ާᴹ䚇ՐਟӢਸᙗǄ൘໘㾯କ઼ডൠ傜᣹ˈᆳԜ൘䐍⿫ 䖳䘁ǃަᆳᶑԦ㢟ྭᰦˈਟԕ㠚⭡ᵲӔǄն໘㾯କ⦹㴰哽Ⲵ㠚❦࠶ᐳӵተ䲀Ҿ⋯ ⵰໘㾯କ઼ডൠ傜᣹㾯䜘ፆඑⲴᆓ㢲ᙗᒢ⠕Ⲵǃ༿ᆓᴹ䴘Ⲵӊ✝ᑖൠ४઼໘㾯କ ѝ䜘儈৏ൠ४˄Wilkes, 1972; Gonzalez and Corral, 1997˅ǄӪᐕ䟷䳶໘㾯କ⦹㴰哽㣡㊹㔉⦹㊣ᵲӔ⿽઼ᔰ᭮ᦸ㊹㗔փᦸ㊹ˈ⇿ょᒣ൷ਟԕ࠶࡛ᗇࡠ 8 ㋂઼ 12 ㋂⿽ ᆀˈ㔃ᇎ⦷㓖Ѫ 1-2%˄Baltazar et al., 2005˅Ǆ৽䗷ᶕˈ⭘⦹㊣㣡㊹㔉໘㾯କ⦹㴰哽ᦸ㊹㔃ᇎቡՊ⴨ᖃഠ䳮ˈ⇿ょ㔃ᇎᴰཊҏቡᱟ 0.2-0.3 ㋂˄Baltazar et al., 2005; Ellstrand et al., 2007˅Ǆ ⦹㊣઼᪙ᬖ⿮኎ਟᵲӔˈնᶱѪн᱃ǄᒦфᵲӔ⿽ާᴹ儈ᓖⲴн㛢ᙗˈ൘䚇 Րᆖкҏнっᇊ˄Mangelsdorf, 1974˅ǄGalinat˄1988˅Ⲵ⹄ウ䘋а↕䈤᰾ˈ⭡Ҿ ᪙ᬖ⿮኎઼⦹㊣ḃ㢢փᮠⴞн਼ˈ␫࣐ањཊ։Ⲵ᪙ᬖ⿮኎ḃ㢢փࡠ⦹㊣สഐ㓴 Ⲵࠐ⦷ᱟᖸվⲴˈഐ↔ḃ㢢փ䰤ⲴӔᦒ⦷ᶱվǄ 1.2.6 㛨ᙝδਥ㛨䘎ᱥу㛨θ㛨ᙝ儎քθྸ᷒у㛨θᓊ䈪᱄ኔ֋〃у 㛨㊱ශε ਇփ⦹㊣ਟ㛢ˈ൘㠚❦ᶑԦлާᴹᦸ㊹ǃਇ㋮઼㔃ᇎ㜭࣋Ǆնᴹ䇨ཊഐ㍐ ਟԕᕅ䎧⦹㊣Ⲵ䳴㣡н㛢ˈྲ儈⑙ǃᒢᰡǃ䗀ሴǃॆᆖ㦟⢙༴⨶ԕ৺㩕ޫݳ㍐㕪ѿㅹǄ 1.2.7 ޞ⭕㛨ᵕ ⑙ᓖ઼⭏䮯ਁ㛢Ⲵޣ㌫˖ ⦹㊣ᱟаᒤ⭏Ἵ⢙ˈަ⭏㛢ᵏⲴ䮯⸝ਆߣҾ૱⿽⢩ᙗ৺ަ⭏䮯Ⲵ⧟ຳǄ⭏䮯 ⛩䵢ࠪൠ䶒˄5 ⡷ኅᔰਦࡠ 7 ⡷ኅᔰਦ䱦⇥˅ԕਾˈ⦹㊣൘վҾ 0°C Ⲵ⑙ᓖлᆈ ⍫ᰦ䰤н䎵䗷 6~8 ሿᰦˈ߫ᇣ〻ᓖਆߣҾ 0°C ԕл⑙ᓖᤱ㔝Ⲵᰦ䰤䮯⸝ǃ൏༔ᶑ Ԧǃ↻㥜ǃվ⑙ᵏⲴ䮯⸝ǃ仾ੁǃ⴨ሩ⒯ᓖ઼Ἵ⢙ਁ㛢䱦⇥Ǆ൘⑙ᑖൠ४ᲊ᱕ᰦ Ⲵ䖫䵌߫ਟԕᕅ䎧ਦ⡷ᦏՔˈҏᖡ૽⦹㊣♼⍶Ǆഐ↔ˈ⦹㊣㜭੖ᆼᡀ⭏ભઘᵏਆ ߣҾᰐ䵌ᵏⲴ䮯⸝Ǆа㡜ᰙ⟏૱⿽ࡠᲊ⟏૱⿽ޘ⭏㛢ᵏ㓖Ӿ 90 ཙ~ 130 ཙнㅹǄ ᰐ䵌ᵏⲴ䮯⸝ߣᇊҶн਼Ⲵ⦹㊣૱⿽Ⲵ⭏㛢ᵏ䮯⸝н਼Ǆ⴨ᓄⲴᡀ⟏ᵏҏਆ ᶑԦǃൠᖒǃབྷර≤փ઼൏༔㊫ර˄Troyer, 1994˅Ǆى≄ߣҾ⭏㛢ᵏ䰤Ⲵ ਼а૱⿽Ⲵᡀ⟏ᵏ൘਼аᒤн਼Ⲵ⿽Ἵൠ⛩ᴹᡰн਼ˈ൘਼аൠ⛩н਼ᒤԭ ҏ〽ᴹн਼ˈ䘉ਆߣҾӾ⿽Ἵࡠ᭦㧧㓿শⲴ⧟ຳᶑԦǄ 27 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⭏䮯ਁ㛢䱦⇥˖ ⦹㊣㠚᫝⿽㠣ᡀ⟏㓿শҶ㩕ޫ⭏䮯˄V˅઼⭏⇆⭏䮯˄R˅䱦⇥˄Kumudini and Tollenaar, 1998˅Ǆ䘉њ䗷〻ਟ䘋а↕࠶Ѫ䇨ཊ䱦⇥˄മ 4˅˖

മ4. ⦹㊣⭏䮯ਁ㛢䱦⇥⽪᜿മ 㤇ᵏ˄VE~V5˅˖⭡Ҿѝ㜊䖤䗵䙏⭏䮯ˈ⦹㊣ࠪ൏Ǆ䲿ਾˈ㜊ਦᔰ࿻䙊䗷㜊㣭䷈ቆ䮯ࠪᶕˈ50%ԕк⦹㊣㤇儈 2~3 cm ԕк〠Ѫࠪ㤇Ǆ㤇ᵏᱟԕ⭏ṩǃ䮯ਦǃ 㤾㢲࠶ॆѪѫⲴ㩕ޫ⭏䮯䱦⇥ˈа㡜শᰦ 20~35 ཙнㅹǄ ᤄ㢲ᵏ㠣ᣭ䳴ᵏ˄V6~VT˅˖⦹㊣⭏䮯⛩սҾൠ䶒ԕкˈ㤾䗵䙏ը䮯֯⦹㊣ ᤄ㢲Ǆᤄ㢲ᱟ⭡⦹㊣⭡㩕ޫ⭏䮯䖜ੁ㩕ޫ⭏䮯о⭏⇆⭏䮯ᒦ䘋ⲴᰦᵏǄ㠣ᴰਾа ⡷ਦ䮯ࠪˈ䲿ਾ䳴ょࠪ⧠ˈ⦹㊣䗮ࡠᴰབྷṚ儈Ǆа㡜শᰦ 27~30 ཙǄ ੀэᵏ˄R1˅˖ᖃ㣡э൘㤎ਦཆ䶒ਟ㿱ˈᣭэণ੺ᔰ࿻ˈ൘䘉а䱦⇥㜊⨐ཆ 㿲ⲭ㢢ˈ޵䜘␵Რਟ㿱ˈਜ਼ᴹᖸቁⲴ⏢փˈ㜊нਟ㿱Ǆ൘ᣭэࡽਾⲴҼઘᱟ⦹㊣ ἽṚⲴ⭏ભઘᵏ䟼ሩ⧟ຳ䘶ຳᴰ᭿ᝏⲴ䱦⇥Ǆ൘䘉а䱦⇥ˈ൘䘶ຳᶑԦлᑨ㿱㊭㋂䍕㛢Ǆ䍕㛢䙐ᡀⲴ㊭㋂ᮠⴞⲴ߿ቁՊ䙐ᡀѕ䟽߿ӗǄ ≤⌑ᵏ˄R2˅˖ੀэਾབྷ㓖 10~14 ཙˈ≤⌑ᵏণᔰ࿻Ǆަ⢩⛩ᱟ㊭㋂Ⲵょ䖤 кᴹ␑ⲭ㢢Ⲵ≤⌑⣦㊭㋂ˈ䟼䶒Ⲵ㜊ңਜ਼ᴹ䘿᰾Ⲵ⏢փˈ㘼㜊ᖒ⣦䖳ሿնਟԕ࠶ 䗘ࠪᶕǄ㣡ᐢ㓿ᆼᡀҶᦸ㊹ਇ㋮࣏㜭ˈᔰ࿻ᒢᷟǄ ң⟏ᵏ˄R3˅˖㣡эᖒᡀਾབྷ㓖 18~22 ཙˈ㊭㋂ཆ㿲Ѫ哴㢢ˈ䲿⵰⏰㊹൘㜊 ң䟼ᔰ࿻〟㍟ˈަ޵Ѫң⣦ⲭ㢢⏢փǄ↔ᰦ䙊䗷䀓ࢆˈ㜊᱃Ҿ䗘䇔Ǆң⟏ᵏょ㋂ ᮠᐢ㓿⺞ᇊˈ㊭㋂ѝᒢ⢙䍘Ⲵ〟㍟ᱟᖡ૽ӗ䟿Ⲵѫ㾱ഐ㍐Ǆ ֯ˈ㵑⟏ᵏ˄R4˅˖㣡эᖒᡀਾབྷ㓖 24~28 ཙˈ䲿⵰⏰㊹൘㜊ң޵ᤱ㔝〟㍟㊭㋂޵䜘ң⣦⏢փਈぐˈᖒᡀ“ഒṧ”⏢փˈ㵑⟏䱦⇥ণ੺ᔰ࿻Ǆ ࠩ䲧ᵏ˄R5˅˖㣡эᖒᡀਾབྷ㓖 35~42 ཙˈޘ䜘ᡆ䜘࠶㊭㋂亦䜘ᔰ࿻ࠩ䲧ˈ ൘㊭㋂Ⲵࠩ䲧ㄟ䱴䘁ˈࠪ⧠Ҷаᶑ᰾ᱮⲴ≤ᒣ㓯ˈᆳᱟᡀ⟏㊭㋂⏢փ˄ң˅४઼ പփ˄⏰㊹˅४Ⲵ࠶⭼㓯ˈ〠֌“ң㓯”Ǆ䲿⵰㊭㋂Ⲵᡀ⟏ˈ“ң㓯”ሶੁ⵰㊭㋂Ⲵ ส䜘˄ょ䖤ᯩੁ˅〫ࣘǄᖃң㓯൘㊭㋂Ⲵ亦䜘઼ส䜘ѻ䰤䗮ࡠ 50%ᰦˈ㊭㋂ণѪ 40~45%ਜ਼≤䟿ˈᒦфᐢ㓿䗮ࡠҶަᴰ㓸ᒢ⢙䍘䟽Ⲵ 95%Ǆ ㊭㋂ˈᆼ⟏ᵏ˄R6˅˖㣡эᖒᡀਾབྷ㓖 55~65 ཙˈ㊭㋂Ⲵᒢ⢙䍘䗮ࡠᴰབྷ٬ ণ䗮ࡠ⭏⨶ᙗᡀ⟏Ǆ൘㊭㋂Ⲵң㓯⎸ཡਾнѵˈ㊭㋂ส䜘ᖒᡀ唁ቲˈቡ䗮ࡠҶᆼ 28 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⟏ᵏ䱦⇥Ǆ唁ቲ俆ݸ൘᷌ょ亦䜘㊭㋂ࠪ⧠ˈ䲿ਾส䜘㊭㋂䙀↕ӗ⭏Ǆ唁ቲࠪ⧠ਾ ㊭㋂Ⲵᒣ൷ਜ਼≤䟿Ѫ 30~35%ˈնਟԕ䲿⵰⧟ຳᶑԦⲴн਼㘼ᴹᡰਈॆǄ 1.2.8 ൞㠠❬⮂ѣ⭕ᆎ㑷⇌Ⲻ㜳࣑θऻᤢ䏀ߢᙝȽ䏀ཅᙝ਀ᣍ䘼ᙝㅿ ㊣㓿䗷䮯ᵏⲴ傟ॆˈ֯⿽ᆀᡀѪୟа㑱⇆ಘᇈˈᒦ䴰㾱Ӫ㊫Ⲵᑞࣙ֯ަ㔗⦹ 㔝цԓ㑱㹽ᡆ㘵オ䰤Ր᫝˄Gould, 1968; OECD, 2002˅Ǆ൘㠚❦⭏ຳ䟼ˈ⦹㊣ᱟ 㻝ᙗⲴ⢙⿽ˈᒦфᐢ㓿їཡҶަ൘䟾⭏ᶑԦлᆈ⍫Ⲵ㜭࣋˄Gould, 1968˅Ǆץ䶎 ਼ᵲ㥹Ἵ⢙⴨∄ˈ⦹㊣ᴹ䳼ᙗ㣡ᒿ˄᷌ょ˅ˈょ䖤㻛㤎ਦव㼩ǄҾᱟˈа㡜н㜭 ਁ⭏অ㋂⿽ᆀⲴՐ᫝Ǆ ⦹㊣нާᰐᙗ㑱⇆㜭࣋ˈ⿽ᆀнާՁⵐ⢩ᙗǄ⦹㊣⿽ᆀⲴᆈ⍫ਆߣҾ⑙ᓖǃ ↔ᣔ઼⿽ᆀᡀ⟏ᓖㅹǄ⑙ᓖ䱽ࡠ 0°C ԕлՊᖡ૽㨼ਁˈഐ؍⒯ᓖǃสഐරǃ᷌Ⳟ վ⑙ᱟ⿽ᆀ⭏ӗᵏ䰤Ⲵѫ㾱仾䲙Ǆ儈Ҿ 45°C Ⲵ⑙ᓖҏՊሩ⿽ᆀⲴ⭏⍫࣋ӗ⭏н 㢟ᖡ૽˄Shaw, 1988˅Ǆ≤࠶൘⦹㊣Ⲵ⭏䮯ᆓ㢲ᱟ䲀ࡦᙗഐ㍐ˈቔަ൘⦹㊣Ⲵᔰ 㣡ᵏ઼♼⍶ᵏˈ≤࠶н䏣ਟԕሬ㠤ᦸ㊹ഠ䳮ᡆ㜊㛾䍕㛢˄Campos et al., 2006˅Ǆ 㲭❦൘ка⭏䮯ᆓ㢲⍂㩭Ⲵ⦹㊣ਟԕ䎺ߜᒦᴹਟ㜭൘лᒤ㨼ਁˈնᱟᆳн㜭 ↔ᵲ㥹аṧ䮯ᵏᆈ⍫Ǆ൘䇨ཊߌ⭠㌫㔏䟼ˈᑨ㿱Ⲵ⦹㊣㠚⭏㤇ᖸᇩ᱃৫䲔Ǆഐۿ ⿫ᔰӪᐕṭษ㇑⨶ˈ⦹㊣н㜭䮯ᵏᤱ㔝㑱⇆˄Gould, 1968˅Ǆ 1.3 ਍։ὃ⢟Ⲻ⭕ᘷ⧥ູ 1.3.1 ൞ള޻Ⲻ൦⨼࠼ᐹૂ㠠❬⭕ູ ⦹㊣൘ѝഭ࠶ᐳᖸᒯˈই䎧⎧ইዋˈेࡠे㓜 50°Ⲵ唁嗉⊏ˈь䎧ਠ⒮৺⋯ ⎧਴ⴱˈ㾯ࡠᯠ⮶ǃ䶂㯿儈৏ˈ䜭ᴹ⦹㊣⿽ἽǄьे৺㾯ই儈ሂኡ४Ѫ᱕᫝⦹㊣ˈ ᶑԦǃى≄哴␞⎧⍱ฏѪ༿᫝⦹㊣ˈ൘ᒯ㾯ǃ⎧ইㅹⴱਟаᒤєᆓ⿽ἽǄṩᦞ਴ൠ ⭏ӗᶑԦ઼⿽ἽࡦᓖˈӾьेࡠ㾯ইⲴ⤝䮯४ฏ޵ˈᖒᡀҶѝഭ⦹㊣Ⲵѫ㾱⿽Ἵ ४˄മ 5˅ˈণेᯩ᱕⦹㊣४ǃ哴␞⎧༿⦹㊣४ǃ㾯ই⦹㊣४ǃইᯩ⦹㊣४઼㾯 े⦹㊣४Ǆ ेᯩ᱕⦹㊣४˖Ѫᡁഭѫ㾱⦹㊣ѫӗ४ˈঐޘഭ᫝⿽ᙫ䶒〟Ⲵ 39.2%ˈঐޘ ഭᙫӗⲴ 43.8%Ǆ䈕४वᤜ唁嗉⊏ǃਹ᷇ǃ䗭ᆱǃᆱ༿ˈ޵㫉ਔǃኡ㾯བྷ䜘ˈ⋣ ेǃ䲅㾯઼⭈㚳े䜘Ǆ⭏ᘱᶑԦ઼⭏ӗᶑԦ䜭䘲ਸ⦹㊣⭏䮯ˈൠ࣯ᒣඖˈ൏༔㛕 ݹ➗ݵ䏣ˈᰐ䵌ᵏ 130~170 ཙˈᒤ䱽䴘䟿 400~800 mm˄60%䱽䴘൘ 6~9 ᴸˈ⊲ 䰤˅Ǆ኎Ҿаᒤа⟏ࡦൠ४Ǆेᯩ᱕⦹㊣४ѫ㾱वਜ਼˖ेᯩᶱᰙ⟏᱕⦹㊣㊫ර४ǃ ेᯩᰙ⟏᱕⦹㊣㊫ර४ǃьॾेѝᰙ⟏᱕⦹㊣㊫ර४ǃьॾेѝ⟏᱕⦹㊣㊫ර४ ઼ьॾेѝᲊ⟏᱕⦹㊣㊫ර४ㅹӄњ⦹㊣઼䶂䍞⦹㊣㊫ර४ˈԕ৺ेᯩ勌伏⭌⦹ ㊣ǃ勌伏㌟⦹㊣㊫ර४઼⠶㻲⦹㊣㊫ර४Ǆ 哴␞⎧༿⦹㊣४˖Ѫᡁഭ⦹㊣Ⲵѫӗ४ˈঐޘഭ᫝⿽ᙫ䶒〟Ⲵ 32.7%ˈঐޘ ഭᙫӗⲴ 35.5%Ǆ䈕४वᤜኡь઼⋣ইǃ⋣ेབྷ䜘ǃኡ㾯ѝই䜘ǃ䲅㾯ޣѝൠ४ ઼⊏㣿ᗀ␞ൠ४ˈᱟ⦹㊣Ⲵ䳶ѝӗ४Ǆ≤䍴ⓀѠᇼˈ哴⋣ǃ␞⋣઼⎧⋣⁚ク䈕४ˈ ᒤ䱽䴘䟿Ѫ 500~900 mm˄70%䱽䴘൘༿ᆓ˅ˈ♼Ⓓ䶒〟䗮 50%ˈ኎Ҿаᒤє⟏ࡦ ൠ४Ǆ哴␞⎧༿⦹㊣४ѫ㾱वਜ਼˖哴␞⎧༿⦹㊣㊫ර४ǃӜ⍕ްᰙ⟏༿⦹㊣㊫ර

29 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ४ㅹєњᲞ䙊⦹㊣઼䶂䍞⦹㊣㊫ර४ˈԕ৺哴␞⎧勌伏⭌⦹㊣ǃ勌伏㌟⦹㊣㊫ර ४઼⠶㻲⦹㊣㊫ර४Ǆ 㾯ই⦹㊣४˖वᤜമ 5 ѝⲴ㾯ইኡൠш䲥⦹㊣४઼䶂㯿儈৏⦹㊣४єњӊ४Ǆ ަѝ䶂㯿儈৏⦹㊣४սҾ儈㓜ᓖൠ४ˈवᤜ䶂⎧ⴱ઼㾯㯿㠚⋫४ǃഋᐍ㾯䜘઼Ӂ ইे䜘ൠ४Ǆ⦹㊣ṭษশਢ䖳⸝ˈ᫝⿽䶒〟ᖸሿǄ㾯ইኡൠ⦹㊣४वᤜഋᐍǃӁ ই઼䍥ᐎⴱǃ䲅㾯ই䜘ǃᒯ㾯㾯䜘儈৏ൠ४ǃ⒆ইǃ⒆े઼⭈㚳䜘࠶ൠ४Ǆኡൠ ঐ䈕४Ⲵ 90%ˈൠᖒ༽ᵲǄᰐ䵌ᵏѪ 240~330 ཙˈ䱽䴘䟿Ѫ 800~1200 mmǄ䈕 ४⿽ἽⲴ⦹㊣᱃ਇ⭏⢙઼䶎⭏⢙㛱䘛˄ྲᒢᰡ઼⯵㲛ᇣ˅ⲴডᇣǄ኎Ҿаᒤа⟏ ᡆаᒤཊ⟏ࡦൠ४Ǆҏ኎ҾᡁഭⲴ⦹㊣ѫӗ४ˈঐޘഭ᫝⿽ᙫ䶒〟Ⲵ 18.4%ˈঐ ޘഭᙫӗⲴ 13.4%Ǆ㾯ই⦹㊣४ѫ㾱वਜ਼˖㾯ই᱕⦹㊣㊫ර४ˈ㾯ই勌伏⭌⦹㊣ǃ 勌伏㌟⦹㊣㊫ර४ˈ⠶㻲⦹㊣㊫ර४ㅹǄ ইᯩ⦹㊣४˖䈕४䶒〟ᒯབྷˈवᤜᒯьǃ⎧ইǃ⎉⊏ǃ⊏㣿઼ᆹᗭই䜘ǃᒯ 㾯ь䜘ǃ⒆ই઼⒆ेⴱǄ䈕४ѫ㾱⿽Ἵ≤にˈ⦹㊣䶒〟䖳ሿˈਚঐޘഭ᫝⿽䶒〟 Ⲵ 3.2%ˈӗ䟿ਚঐޘഭᙫӗⲴ 2.2%Ǆ䈕४սҾ✝ᑖ઼ӊ✝ᑖൠ४ˈᰐ䵌ᵏ 220~360 ཙˈ䱽䴘䟿 1000~1800 mmˈ䘲ਸ⦹㊣⭏䮯Ǆ኎Ҿаᒤй⟏ᡆаᒤഋ⟏ࡦൠ४Ǆ ইᯩ⦹㊣४ѫ㾱वਜ਼˖✝ᑖӊ✝ᑖ⦹㊣㊫ර४ǃьই᱕⦹㊣㊫ර४ˈԕ৺ьই勌伏⭌⦹㊣ǃ勌伏㌟⦹㊣㊫ර४ㅹǄ 㾯े⦹㊣४˖䈕४Ѫᒢᰡൠ४ˈ䱽䴘䟿վҾ 200 mmˈवᤜᯠ⮶ǃ⋣㾯⭈㚳䎠ᓺǃᆱ༿⋣྇ൠ४Ǆ䘉аൠ४Ⲵ֌⢙ѫ㾱䶐♼ⒹǄᰐ䵌ᵏ 130~180 ཙǄ⦹㊣᫝ ⿽䶒〟ঐޘഭ᫝⿽䶒〟Ⲵ 3%Ǆ኎Ҿаᒤа⟏ࡦൠ४Ǆ㾯े⦹㊣४ѫ㾱वਜ਼˖㾯 े᱕⦹㊣㊫ර४ˈԕ৺⠶㻲⦹㊣㊫ර४ㅹǄ

മ5. ѝഭ⦹㊣⿽Ἵ४ࡂമ

30 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 1.3.2 ⭕䮵ਇ㛨ᡶ㾷≸Ⲻ⭕ᘷ⧥ູᶗԬθऻᤢ㠠❬ᶗԬૂṳฯᶗԬⲺ ᭯਎ሯެ൦⨼࠼ᐹ॰ตૂ㤹പᖧଃⲺਥ㜳ᙝ 䘋⿽ᆀ㨼ਁ઼ࠪ׳㊣⭏䮯ਁ㛢⨶ᜣⲴ⭏ᘱᶑԦवᤜݵ䏣Ⲵ൏༔ਜ਼≤䟿ǃ㜭⦹ ൏Ⲵᴰ֣൏༔⑙ᓖ઼ݵ䏣Ⲵ൏༔ޫ࠶Ǆ⦹㊣⭏ӗⲴᴰ֣ᰦᵏᱟᴰ✝ᴸԭⲴㅹ⑙㓯 ൘ 21~27 C ѻ䰤ˈᰐ䵌ᵏ൘ 120~180 ཙѻ䰤Ǆ15 cm Ⲵ༿ᆓ䱽䴘䟿㓖Ѫᰐ♼Ⓓᶑ Ԧл⦹㊣⭏ӗⲴ䱽䴘л䲀ˈ⦹㊣⭏䮯ᵏ䰤Ⲵ䱽䴘䟿㾱≲㲭ᰐк䲀ˈն䗷ཊⲴ䱽䴘 Պሬ㠤߿ӗǄ ⑙ᓖᱟ⦹㊣⭏䮯Ⲵањޣ䭞ഐ㍐Ǆ⑙ᓖ䱽ࡠ 0°C ԕлՊᖡ૽⿽ᆀⲴ㨼ਁˈഐ ↔վ⑙ᱟ⿽ᆀ⭏ӗᵏ䰤Ⲵѫ㾱仾䲙Ǆ儈Ҿ 45°C Ⲵ⑙ᓖҏՊሩ⿽ᆀⲴ⭏⍫࣋ӗ⭏ н㢟ᖡ૽˄Shaw, 1988˅Ǆ൘⦹㊣䗮ࡠ⭏⇆⭏䮯䱦⇥˄ᣭょ㠣ᔰ㣡ᵏ˅ਾˈᤱ㔝 Ⲵ儈⑙Պ߿ቁ㣡㊹Ⲵ⍫઼࣋ӗ䟿Ǆ≤࠶൘⦹㊣Ⲵ⭏䮯ᆓ㢲ᱟ䲀ࡦᙗഐ㍐ˈቔަ൘ ⦹㊣Ⲵᔰ㣡ᵏ઼♼⍶ᵏˈ≤࠶н䏣ਟԕሬ㠤ᦸ㊹ഠ䳮ᡆ㜊㛾䍕㛢˄Claassen and Shaw, 1970; Boyer and Westgate, 2004; Campos et al., 2006˅Ǆ ഐ↔ˈ䙊䗷䈳ᮤṭษ᧚ᯭˈྲӪᐕ♼Ⓓㅹˈਟԕᢙབྷ⦹㊣Ⲵ⿽Ἵ४ฏ઼㤳തǄ 1.3.3 ᱥੜѰ⭕ᘷ⧥ູѣⲺ㓺ᡆ䜞࠼ 㓿䗷䮯ᵏⲴӪᐕṭษ઼傟ॆˈ⦹㊣ᐢн޽ᱟ㠚❦⭏ᘱ⧟ຳѝⲴ㓴ᡀ䜘࠶ˈ 㘼ᱟߌъ⭏ᘱ㌫㔏ѝањ䟽㾱㓴ᡀ䜘࠶Ǆ↓ྲ൘ 1.1.7 㢲䟼䇘䇪Ⲵ䛓ṧˈ⦹㊣ᰙ ൘ 16 ц㓚ቡᶕࡠҶѝഭˈᱟᰙᵏⲴ⅗⍢Րᮉ༛ᑖᶕⲴǄⴞࡽˈ⦹㊣൘ѝഭⲴཊ ᮠⴱԭ䜭ᴹ⿽Ἵ˄1.3.1 㢲˅Ǆྲ൘ 1.2.8 ѝᡰᤷࠪⲴˈ⦹㊣н㜭൘䶎Ӫᐕṭษ ᶑԦл⭏ᆈǄ 1.3.4 ф⭕ᘷ㌱㔕ѣެᆹὃ⢟Ⲻ⭕ᘷީ㌱θऻᤢ⭕ᘷ⧥ູⲺ᭯਎ሯ䘏 ᓭڛ㊱δӑεީ㌱Ⲻᖧଃԛ਀ᱥੜՐഖ↚㙂ӝ⭕ᡌ໔ࣖሯӰ〃 ૂ⭕ᘷ⧥ູⲺу࡟ᖧଃ ྲкᡰ䘠ˈḀӋ໘㾯କ⦹㴰哽⢙⿽ਟԕ਼⦹㊣ᵲӔӗ⭏ਟ㛢ਾԓˈնᱟ໘ 㾯କ⦹㴰哽ਚ⭏䮯൘໘㾯କ઼ডൠ傜᣹˄Sánchez-González and Ruiz-Corral, 1997˅Ǆ᪙ᬖ⿮኎ⲴḀӋ⢙⿽ਟԕ਼⦹㊣ᵲӔˈնᶱѪн᱃ǄᒦфᵲӔ⿽ާᴹ 儈ᓖⲴн㛢ᙗˈнާᴹ䚇Րᆖっᇊᙗ˄Mangelsdorf, 1974˅Ǆ↔ཆˈ⦹㊣⋑ᴹ 㻝⧠ᴹⲴ⭏ᘱ㌫㔏Ǆഐ↔ˈ⭏ᘱ⧟ຳⲴץн㜭ᴹ᭸ൠˈੁٮᵲ㥹⢩ᙗ઼ᵲ㥹ॆ ⢙㻝ᙗᵲ㥹ᡆ䙐ᡀ〰ᴹץ᭩ਈሶнਟ㜭ሩ⦹㊣ӗ⭏䗷ཊⲴ⭏ᘱ᭸ᓄˈྲ╄ॆѪ ⿽☂Ҿ⚝㔍ㅹǄ ⦹㊣ᱟа⿽ᔲӔ֌⢙Ǆྲ൘ 1.3.1 ѝᨀࡠⲴˈѝഭ਴њൠ४䜭ᴹ⦹㊣Ⲵ⿽ἽǄ 亴䇑⦹㊣઼ަԆ֌⢙ᡆἽ⢙Ⲵ⭏ᘱᆖޣ㌫нՊ⭡Ҿ⭏ᘱ⧟ຳⲴਈॆ㘼ਁ⭏᭩ਈǄ 1.3.5 ф⭕ᘷ㌱㔕ѣެᆹ⭕⢟δࣞ⢟ૂᗤ⭕⢟εⲺ⭕ᘷީ㌱θऻᤢ⭕ ᘷ⧥ູⲺ᭯਎ሯ䘏〃δӑεީ㌱Ⲻᖧଃԛ਀ᱥੜՐഖ↚㙂ӝ⭕ ᓭᡌ⭕ᘷ⧥ູⲺу࡟ᖧଃڛ㊱ᡌ໔ࣖሯӰ ൘⭏ᘱ㌫㔏ѝˈ⦹㊣਼൏༔޵ཆⲴަԆ⭏⢙ᴹаᇊⲴ⭏ᘱᆖޣ㌫Ǆ⦹㊣ṩ 31 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ㌫⭡Ҿަ਼䈨ཊᗞ⭏⢙㗔փˈྲ㓶㧼ǃⵏ㧼ǃ᭮㓯㧼˄Vega-Segovia and Ferrera-Cerrato, 1996˅ǃ৏⭏ࣘ⢙઼㷘㊫ㅹᴹаᇊⲴޣ㌫ˈᡰԕ䘈㜭䎧ࡠ൏༔ ㌫˗⦹㊣਼ᰦҏᴹаӋᑨ㿱Ⲵ⯵ޣᤱ⭏ᘱ؍䈳㢲Ⲵ֌⭘ǄаӋᴹ⳺ᰶ㲛਼⦹㊣㲛ᇣǄ䬸⯵ǃ唁㊹⯵ǃਦᯁ⯵઼㤾㞀⯵ㅹ䜭ᱟ⦹㊣кⲴᑨ㿱⯵ᇣˈަਁ⭏ᛵߥ ḃ⦹㊣ˈ㘼ᆳԜӗץ㓿⍾ᆖ᜿ѹഐഭᇦ㘼ᔲǄྲᴢ䴹㧼઼䮠࠰㧼ㅹⵏ㧼ҏՊ઼ ץ⭏Ⲵ䴹㧼∂㍐Պᖡ૽Ӫ㊫઼⢢⮌Ⲵ伏⭘Ǆ㲛ᇣ઼ᒢᰡㅹ䘶ຳਟԕ࣐ࢗⵏ㧼Ⲵ ḃ˄Dowd, 2001˅Ǆ ⦹㊣Ⲵѫ㾱ᇣ㲛ᶕ㠚Ҿ匎㗵ⴞ઼䷈㗵ⴞᰶ㲛Ǆ匎㗵ⴞⲴᒬ㲛ྲ⦹㊣㷏ਟԕ ਆ伏⦹㊣Ⲵਦ⡷ǃ㤾઼ょ䜘Ǆ䷈㗵ⴞⲴᒬ㲛ྲ⦹㊣࠷ṩਦ⭢ਟԕਆ伏ṩ䜘ǃ㤾ǃ 㣡э઼㣡㊹ˈᡀ㲛ਟԕਆ伏ਦ䜘˄Dicke and Guthrie, 1988˅Ǆ ਼⦹㊣а䎧ᆈ൘Ⲵ⭏⢙о⦹㊣Ⲵ⭏ᘱᆖޣ㌫ˈަԫօⲴਈॆˈ亴䇑ሶнՊ ᓧᡆ⧟ຳӗ⭏ԫօⲴ䍏䶒ᖡ૽ǄڕሩӪփ 1.3.6 ሯ⭕ᘷ⧥ູⲺᖧଃ਀ެ▒൞ধ䲟ぁᓜ ⦹㊣ᱟṭษ֌⢙ˈ൘ѝഭާᴹ䮯ᵏⲴṭษশਢˈሩ⭏ᘱ⧟ຳнՊ䙐ᡀ仾䲙Ǆ 1.3.7 ⎿਀ࡦള޻䶔䙐ᑮ〃ὃⲺὃ⢟⢟〃ᰬθᓊᨅ䘦䈛ὃ⢟Ⲻ㠠❬⭕ ູૂᴿީެཟ❬ᦋ伕㘻Ƚᇺ⭕⢟Ƚㄔҿ⢟ૂާ⭕⢟Ⲻ䍺ᯏ ⦹㊣ާᴹ䮯ᵏⲴṭษশਢˈᱟѝഭഋབྷ֌⢙ѻаˈᱟ䟽㾱Ⲵ㋞伏઼侢ᯉ֌ ⢙ˈ൘ѝഭᲞ䙽⿽ἽǄഐ↔н኎Ҿ䶎䙊ᑨ⿽ἽⲴἽ⢙⢙⿽Ǆ 1.4 ਍։ὃ⢟Ⲻ䚍Ֆ਎ᔸ 1.4.1 䚍Ֆどᇐᙝ ⦹㊣ᱟ儈ㅹἽ⢙ѝᓄ⭘䖳ᒯⲴ䚇Րᆖ⹄ウ઼ᓄ⭘ᶀᯉǄ⦹㊣ᱟᴰᰙ൘ᇎ傼 ᇔѝᔰ࿻䚇Ր⹄ウⲴ֌⢙ѻаǄᰙᵏӪԜሩ⦹㊣㓶㜎Ⲵᴹэ࠶㻲ǃ߿ᮠ࠶㻲ǃ ḃ㢢փ࠶⿫о䘎䬱઼䖜ᓗᆀ᭸᷌ㅹ䚇Ր⧠䊑䜭䘋㹼Ҷᒯ⌋Ⲵ⹄ウǄ⭡Ҿ⦹㊣൘ 㖾ഭ઼ц⭼㓿⍾ѝᢞ╄⵰䟽㾱Ⲵ䀂㢢ˈᡰԕ⦹㊣Ⲵ䚇Րᆖ⹄ウ㠣Ӻӽਇࡠ䟽㿶Ǆ བྷ䟿Ⲵ䚇Րᆖ⹄ウ㔃᷌㺘᰾ˈ⦹㊣Ⲵᙗ⣦䙊䗷㠚Ӕ઼എӔਟԕっᇊ䚇ՐǄ ⦹㊣䘋ॆᡀѪ㠚⭡ᦸ㊹֌⢙⢙⿽ਾˈⴤࡠҼॱц㓚ˈ⦹㊣ṭษ૱⿽᡽ᡀѪ Ӻཙ᜿ѹкⲴᔲ㣡ᦸ㊹֌⢙ǄକՖᐳਁ⧠ᯠབྷ䱶ѻࡽˈᖃൠⲴ൏㪇Ӫ࡙⭘ㆰঅ Ⲵ㗔փ䘹ᤙˈษ㛢ҶаӋ૱⿽Ǆ൘ӺཙⴻᶕˈԆԜⲴ䘹㛢ᯩ⌅ᶱѪㆰঅˈն䘉 Ӌ䘹ᤙᯩ⌅㜭ॱ࠶ᴹ᭸ൠษ㛢૱⿽ᡆ૱㌫ˈԕ┑䏣ԆԜሩ伏⢙ǃ⟳ᯉǃ侢ᯉ઼ ᮷ॆⲴ䴰≲Ǆ䲿⵰㾯ॺ⨳᮷ॆⲴਁኅˈਁ⧠Ҷ૱⿽䰤ᵲӔ㛢⿽ᯩ⌅ˈ⭡↔ᔰ࿻ Ҷ࡙⭘Ἵ⢙Ⲵ䚇Րਈᔲᶕษ㛢⢩ᇊⲴ૱⿽Ǆ ॱҍц㓚Ҽॱᒤԓˈ、ᆖᇦྐᇊҶ⦹㊣ᵲӔ㛢⿽ส⹰⨶䇪ǄӪԜ䙊䗷䘋㹼 ⦹㊣૱⿽䚇Ր㓴ᡀⲴส⹰⹄ウˈᶕ⺞ᇊḀњ⢩ᇊ⦹㊣૱⿽޵Ⲵ㠚㣡ᦸ㊹᭸ᓄ ˄Shull, 1909˅Ǆᖃሩ⦹㊣অṚ䘎㔝䘋㹼 7 㠣 10 ԓⲴ㠚㣡ᦸ㊹ˈਟԕษ㛢ࠪ㓟㌫˄ণ㠚Ӕ㌫˅ˈ䘉њ㓟㌫㗔փѝⲴ⇿њἽṚ䜭ᴹ⴨਼Ⲵ䚇Ր⢩ᙗǄᆏᗧቄ䚇 Րᆖ⨶䇪ሩ൘㠚Ӕ䗷〻ѝਁ⭏Ⲵ䚇Րਈॆ䘋㹼Ҷ↓⺞䀓䟺˖䙊䗷㠚Ӕˈ֯⇿њ 32 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 สഐս⛩кⲴᵲਸㅹսสഐ䙀↕ਁ⭏㓟ਸˈᖒᡀ㓟㌫Ǆ൘㓟㌫䟼ㅹսสഐⲴ㓟 ਸˈ֯ἽṚⲴ⍫઼࣋⭏ӗ࣋Პ䙽л䱽Ǆ ᓭᡌ⭕ᘷ⧥ູӝ⭕у࡟ᖧଃڛ㊱ᱥੜᴿਇ⭕䚍Ֆ਎ᔸ㙂ሯӰ 1.4.2 Ⲻ䍺ᯏ ൘⦹㊣㛢⿽䗷〻ѝˈ㛢⿽ᇦ࡙⭘䈡ਈǃᵲӔㅹ᡻⇥ࡋ䙐ᯠⲴ䚇Րਈᔲˈ⭘ ᶕ䘹㛢ᯠ૱⿽Ǆ⦹㊣൘㠚❦ᶑԦлҏՊӗ⭏аӋ䚇Րਈᔲˈ㛢⿽ᇦҏ㓿ᑨᴹⴞ ⲴⲴ䘹ᤙǃ࡙⭘䘉Ӌ㠚❦ਈᔲ䘋㹼૱⿽᭩㢟Ǆⴞࡽᵚ㿱䘉Ӌ䚇ՐਈᔲሩӪ㊫ᡆ ⧟ຳӗн࡙ᖡ૽Ⲵ䍴ᯉǄ 1.4.3 ൞㠠❬ᶗԬсфެᆹὃ⢟〃ኔ䘑㺂䚍Ֆ⢟䍞ӚᦘⲺਥ㜳ᙝ Ἵ⢙ᴹਟ㜭ਁ⭏สഐ┲〫ˈᒦ䙊䗷↓ᑨᴹᙗՐ᫝ᇎ⧠สഐ⑀⑇ˈնᗵ享ާ ᆖ⢩ᙗᗵ享ᴹаᇊ䟽ਐˈণى⢙˅аᇊᶑԦ˖1˅єњӢᵜᗵ享ᴹᙗӢਸ˗2༷ 㣡ᵏ⴨䙷˗3˅ᗵ享ᴹ䘲ᖃⲴ㣡㊹䖭փˈᆳ㜭൘єњӢᵜѻ䰤Ր䙂㣡㊹Ǆ ⦹㊣઼аᒤ⭏໘㾯କ⦹㴰哽ާᴹ䚇ՐӢਸᙗǄ൘໘㾯କ઼ডൠ傜᣹ˈ䐍⿫ 䖳䘁ᰦˈ䙊䗷仾ჂՐ㊹ਟԕ㠚⭡ᵲӔ˄Wilkes, 1967; Sanchez et al.,1998˅Ǆն ൘ѝഭ⋑ᴹ໘㾯କ⦹㴰哽Ǆ ൘䟾⭏ᶑԦлˈ⦹㊣਼᪙ᬖ⿮኎⢙⿽ᵲӔᱟᶱѪഠ䳮Ⲵ˄Wilkes, 1972˅Ǆ ഐѪ᪙ᬖ⿮኎Ἵ⢙ሩ⭏ຳⲴ㾱≲㊫լҾ໘㾯କ⦹㴰哽ˈᡰԕ൘ѝഭ⋑ᴹ᪙ᬖ⿮ ኎Ἵ⢙Ǆ ൞㠠❬ᶗԬсфެᆹ⭕⢟δׁྸᗤ⭕⢟ε䘑㺂䚍Ֆ⢟䍞ӚᦘⲺ 1.4.4 ਥ㜳ᙝ ᵚਁ⧠ԫօޣҾ䚇Ր⢙䍘⭡⦹㊣䙊䗷⿽䰤ᴹᙗᵲӔੁަԆ⢙⿽Ր䙂Ⲵᣕ䚃Ǆ ⨶䇪к䇢ˈ䚇Ր⢙䍘䙊䗷≤ᒣՐ䙂ࡠަԆ⭏⢙ᱟਟ㜭Ⲵˈն൘㠚❦ᶑԦлˈӾ Ἵ⢙ੁࣘ⢙ᡆ㘵ᗞ⭏⢙Ⲵ≤ᒣสഐՐ䙂ˈ൘䈅傼кⴞࡽ䘈⋑ᴹᗇࡠ䇱ᇎ ˄Bertolla and Simonet, 1999˅Ǆ 1.5 ਍։ὃ⢟Ⲻⴇ⎁ᯯ⌋ૂⴇ᧝Ⲻਥ㜳ᙝ ㊣Ⲵ⤜⢩䮯⴨ǃ⣦ᘱ઼⭏䮯Ґᙗ֯ަ᱃Ҿ䇶઼࡛ⴁ⍻Ǆ࡙⭘ḀӋ䘹ᤙᙗ⦹ 䲔㥹ࡲ઼ᒯ䉡䲔㥹ࡲˈྲ㥹⭈㟖ǃ䱯⢩᣹⍕ǃ҉㥹㜪ㅹˈਟԕᴹ᭸᧗ࡦߌ⭠㌫㔏ѝнᜣ㾱Ⲵ⦹㊣Ǆ↔ཆˈ䘈ਟԕ࡙⭘ѝ㙅Ⲵᯩ⌅䱢⋫⦹㊣Ⲵ㠚⭏㤇Ǆ 1.6 ਍։ὃ⢟Ⲻެᆹ䍺ᯏ ㊣ⲴަԆ䍴ᯉਟԕ⦹ޣѪᵜ⭣䈧ⴞⲴˈ⋑ᴹᴤཊⲴ䍴ᯉ㾱ᨀ׋Ǆնᴤཊᴹ Ⲵᮉᶀǃ、ᢰҖ㉽ǃ㖁ㄉㅹؑ᚟⑐䚃к㧧ᗇǄޣӾ⴨ 1.7 ṯᦤр䘦䇺ԭθ৸➝ᵢࣔ⌋ㅢॷжᶗᴿީḽ߼ࡈ࠼਍։ὃ⢟Ⲻ ᆿޞㅿ㓝

33 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ሩ⧟ຳ˗ੁٮкᡰ䘠ˈ⦹㊣ሩӪփᡆࣘ⢙⋑ᴹ∂ᙗ˗ᒦфнާᴹᵲ㥹ॆྲ ӗ⭏䍏䶒᭸ᓄᡆ㘵╄ਈᡀᴹᇣ⭏⢙Ⲵਟ㜭ᙗᶱሿǄഐ↔ˈṩᦞߌъ䖜สഐ⭏⢙ ᆹޘ㇑⨶࣎⌅ㅜॱаᶑࡇࠪⲴḷ߶ˈਇփἽ⢙⦹㊣ᓄ኎ᆹޘㅹ㓗 I 㓗Ǆ

34 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᙝ䇺ԭޞะഖᬃ֒Ⲻᆿ .2 侦ᙝ⣬ૂ⢯ᙝⲺਏ䘦ؤᡌޛ䖢ะഖὃ⢟ѣᕋ 2.1 ߌ䗮㊫䲔㥹ࡲѝⲴᴹ᭸ᡀ࠶㥹⭈㟖ᱟ 5-✟䞷щ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦 ˄EPSPS˅ⲴᣁࡦࡲǄ㣣俉᯿≘ส䞨⭏⢙ਸᡀⲴ㧭㥹䞨䙄ᖴᲞ䙽ᆈ൘ҾἽ⢙ǃⵏ 㧼઼㓶㧼ѝˈ㘼 EPSPS ↓ᱟ㧭㥹䞨䙄ᖴѝⲴањޣ䭞䞦Ǆߌ䗮㊫䲔㥹ࡲ࡙⭘䘉 а䙄ᖴˈԕ㥹⭈㟖䱫ᯝἽ⢙փ޵㣣俉᯿≘ส䞨Ⲵਸᡀˈ֯Ἵ⢙ഐ㣣俉᯿≘ส䞨㕪 ཡ㘼↫ӑǄն൘ପңࣘ⢙ǃ呏㊫ᡆ≤⭏⭏⢙ѝˈнᆈ൘㧭㥹䞨䙄ᖴˈഐ↔㥹⭈㟖 ӵ⢩ᔲᙗൠሩἽ⢙ᴹ᭸Ǆ MON 87427 Ⲵᨂޕ DNA ᒿࡇѝवਜ਼ᴹањᆼᮤⲴ cp4 epsp สഐ㺘䗮ⴂˈਟ ԕ㺘䗮 CP4 EPSPS 㳻ⲭǄMON 87427 Ⲵ CP4 EPSPS 㳻ⲭབྷሿ㓖Ѫ 47.6 kDˈਜ਼ ᴹ 455 њ≘ส䞨˄മ 12˅ˈоἽ⢙޵ⓀⲴ EPSPS 㳻ⲭ࣏㜭⴨਼ˈնо㥹⭈㟖ⲴӢ ਸᙗվˈഐ㘼֯ᗇ MON 87427 ਟԕ㙀ਇ㥹⭈㟖㊫䲔㥹ࡲǄ MON 87427 䟷⭘⢩↺੟ࣘᆀ-޵ਜ਼ᆀ㓴ਸ˄e35S-hsp70˅ˈণ໎ᕪර 35S ੟ࣘ ᆀ઼⦹㊣✝◰㳻ⲭ Hsp70 㕆⸱ᒿࡇⲴㅜањ޵ਜ਼ᆀǄ䈕੟ࣘᆀ-޵ਜ਼ᆀ㓴ਸਟԕ ⢩ᔲᙗൠ൘㩕ޫಘᇈ˄ਦ⡷ǃ〨⿶઼ṩ˅઼䳼ᙗ⭏⇆ಘᇈ˄ਟԕ䘋а↕ਁ㛢ᡀ⿽ ᆀᡆ㊭㋂઼㣡эⲴ㓴㓷˅ѝ㺘䗮 CP4 EPSPS 㳻ⲭˈ֯䘉Ӌ㓴㓷઼ಘᇈਟԕᣇ㥹 ⭈㟖Ǆն CP4 EPSPS 㳻ⲭ൘єњޣ䭞Ⲵ䳴ᙗಘᇈѝн㺘䗮ᡆ㺘䗮䟿ᴹ䲀˖㣡㊹ ሿᆒᆀ˄ਟԕ䘋а↕ਁ㛢ᡀ㣡㊹㋂˅઼∑㔂ቲ㓶㜎˄Ѫ㣡㊹㋂ਁ㛢ᨀ׋㩕ޫ˅Ǆ ഐ↔ˈMON 87427 Ⲵ䳴ᙗಘᇈнᣇ㥹⭈㟖Ǆ ⭘ਜ਼ᴹ MON 87427 䖜ॆփⲴ⦹㊣㠚Ӕ㌫֌⇽ᵜˈ⭘ަᆳᣇ㥹⭈㟖Ⲵ⦹㊣㠚 Ӕ㌫֌⡦ᵜˈ൘⦹㊣ V8~V13 ⭏䮯䱦⇥˄䳴ょⲴ⭏䮯ਁ㛢࿻Ҿ㩕ޫ⭏䮯Ⲵ V9 䱦 ⇥˅௧ᯭє⅑㥹⭈㟖䲔㥹ࡲˈਟԕᣁࡦ⇽ᵜⲴ䳴ᙗಘᇈਁ㛢ˈӪᐕࡋ䙐䳴ᙗн㛢Ǆ նਜ਼ᴹަᆳᣇ㥹⭈㟖䖜ॆփⲴ⡦ᵜⲴ䳴ᙗಘᇈਁ㛢ࡉнਇᖡ૽Ǆ⭡↔ᖒᡀн⭘Ӫ ᐕ৫䳴ⲴᵲӔ⿽ࡦ⿽㌫㔏Ǆ 2.2 ᇔ䱻ᨈޛᡌࡖ䲚ᓅࡍⲺԛс䍺ᯏ 2.2.1 ᨈޛᓅࡍⲺཝቅૂ㔉ᶺθ⺤ᇐެ⢯ᙝⲺ࠼᷆ᯯ⌋ MON 87427 ᱟ䙊䗷ߌᵶ㧼ӻሬ䖜ॆˈሶ PV-ZMAP1043 䍘㋂˄മ 6˅ѝⲴ T-DNA ሬޕࡠ LH198 × HiII ਇփ૱㌫ѝ㧧ᗇⲴǄᶕ㠚Ҿ PV-ZMAP1043 䖜ॆ䍘㋂Ⲵ T-DNA वਜ਼ᴹањ cp4 epsps สഐ㺘䗮ⴂˈ䈕สഐ㺘䗮ⴂ⭡ e35S ੟ࣘᆀǃ hsp70 Ⲵ޵ਜ਼ᆀǃCTP2 ਦ㔯փ䖜〫㛭ᒿࡇǃcp4 epsps 㕆⸱ᒿࡇ઼ nos 㓸→ᆀᶴ ᡀǄ Southern ᵲӔ㔃᷌㺘᰾ˈMON 87427 ӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ⦹㊣สഐ㓴Ⲵ অњสഐᓗսкˈᒦф䲔 T-DNA ཆˈ⋑ᴹỰ⍻ࡠ MON 87427 ѝᆈ൘ަᆳᶕ㠚 Ҿ PV-ZMAP1043 䖜ॆ䍘㋂Ⲵᒿࡇ˄Southern ᵲӔ࠶᷀㔃᷌㿱ԕл 2.2.5 䜘࠶˅Ǆ ࡙⭘ PCR ᯩ⌅ᢙ໎Ҷ 3 њ⴨ӂ䟽ਐⲴ DNA ⡷⇥˄മ 7ˈӗ⢙ Aǃӗ⢙ B ઼ 㘬สഐ㓴ᒿࡇǄPCR ᢙ໎ᡰ⭘חᒿࡇ৺ަޕ䮯ᨂޘӗ⢙ C˅ˈ䘉 3 њ⡷⇥⏥ⴆҶ ᕅ⢙઼ᶑԦ㿱㺘 5Ǆሶ䘉 3 њᢙ໎ӗ⢙䘋㹼ᒿࡇ࠶᷀ᒦ᤬᧕ˈণਟ⺞ᇊޘ䮯ᨂޕ

35 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᒿࡇབྷޕ㘬ᒿࡇ˄മ 8˅Ǆṩᦞᒿࡇ࠶᷀㔃᷌ˈMON 87427 Ⲵᨂחᒿࡇ৺สഐ㓴 ሿѪ 3746 bpˈަ ѝ T-DNA ᨂޕѪ 3681 bpǄਖཆ൘ T-DNA Ⲵ 5'ㄟ઼ 3'ㄟ࠶࡛ᴹ 41 bp ઼ 24 bp Ⲵᨂޕˈ䘉ᱟ൘ T-DNA ᨂޕ䗷〻ѝ㠚㹼ᨂޕⲴᒿࡇǄ䘉⿽⧠䊑൘ 䗷〻ѝᑨ㿱ˈਟ㜭ᱟ⭡Ҿৼ䬮ᯝ㻲؞༽ᵪࡦᕅ䎧˄Salomon andޕཆⓀ DNA ᨂ Puchta, 1998˅Ǆ ↔ཆˈᒿࡇ࠶᷀ҏ䇱ᇎ T-DNA ޵ cp4 epsps สഐ㺘䗮ⴂᱟᆼᮤⲴˈަѝ਴䚇 ՐݳԦⲴᧂࡇᯩᔿоަ൘ PV-ZMAP1043 䖜ॆ䍘㋂кⲴᧂࡇᯩᔿа㠤Ǆ ᒿࡇѝޕ㘬ᒿࡇԕ৺㔃ᶴ㿱മ 13 ઼മ 8˗ᨂחᒿࡇ઼สഐ㓴ޕMON 87427 Ⲵᨂ Ⲵ䈳᧗ݳԦ᧿䘠㿱㺘 6Ǆ ˅䶒Ⲵ⭏⢙ؑ᚟ᆖ࠶᷀ˈԕ⺞ᇊ˖1ޘս⛩䱴䘁४ฏ䘋㹼ޕሩ MON 87427 ᨂ MON 87427 ⦹㊣ѝᨂޕ㔃⛩४䱴䘁Ⲵԫօਟ㜭ཊ㛭оᴤᯠⲴ∂㍐㳻ⲭ઼ᣇ㩕ޫ ഐᆀǃ㠤᭿㳻ⲭ઼࣏㜭⍫ᙗ㳻ⲭᮠᦞᓃѝⲴᒿࡇѻ䰤ᱟ੖ᆈ൘⴨լᙗ˗2˅MON 87427 ѝᨂޕⲴ T-DNA 㕆⸱ᒿࡇо∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃ㠤᭿㳻ⲭ઼࣏㜭⍫ ᙗ㳻ⲭᮠᦞᓃѝⲴᒿࡇѻ䰤ᱟ੖ᆈ൘⴨լᙗ˗3˅MON 87427 ᱟ੖⭡ҾཆⓀ DNA Ⲵᨂޕ㘼⹤ൿҶ৏ᴹⲴ⦹㊣޵ⓀสഐⲴ㺘䗮ˈᡆ㘵ӗ⭏ҶᯠⲴᔰ᭮䈫⸱Ṷ˄ORF˅Ǆ ⭏⢙ؑ᚟ᆖ࠶᷀㔃᷌䈖㿱䲿ᵜ⭣䈧䍴ᯉᨀӔⲴᢰᵟᣕ੺ 7Ǆ⭏⢙ؑ᚟ᆖ࠶᷀֯⭘ Ⲵᴰᯠ؞䇒઼ᴤᯠⲴᮠᦞᓃवᤜ˖䗷᭿৏ǃ䞷ⓦ㳻ⲭ઼哖䉧㳻ⲭᒿࡇᮠᦞᓃ ˄AD_2016˅ǃ∂㍐ᮠᦞᓃ˄TOX_2016˅ǃGenBank 㳻ⲭ䍘ᮠᦞᓃ˄PRT_2016˅ǃ GenBank 㺘䗮ᒿࡇḷㆮᮠᦞᓃ˄EST˅ǃ䶎߇։Ṩ㤧䞨ᮠᦞᓃ˄NT_2016˅ԕ৺ 䶎߇։≘ส䞨ᮠᦞᓃ˄NR_2016˅Ǆ ᇊᒿࡇԕ৺Ӿٷ㔃⛩४㘫䈁㘼ᗇⲴ 14 ⿽ޕ㘬ᒿࡇ-ᨂח’⭘Ӿ 5’ᡆ 3֯ T-DNA 㘫䈁Ⲵ 6 ⿽䈫⸱ṶѪỰ㍒ᒿࡇˈ൘ AD_2016ǃTOX_2016 ઼ PRT_2016 ᮠᦞᓃѝ࠶࡛䘋㹼 FASTA ઼ޛ≘ส䞨━ࣘデਓᩌ㍒Ǆᩌ㍒㔃᷌㺘᰾ˈ㲭❦⨶䇪 ˈᇊᒿࡇ઼Ӿ T-DNA 㘫䈁Ⲵ 6 ⿽䈫⸱Ṷ൘Ἵ⢙ѝᆈ൘Ⲵਟ㜭ᙗᶱվٷк䘉 14 ⿽ ᓧⲴ∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃڕ⢙ণ֯ᆈ൘ˈ䘉Ӌᒿࡇоᐢ⸕ਟ㜭ᖡ૽Ӫ㊫ᡆࣘ 㠤᭿㳻ⲭ઼࣏㜭⍫ᙗ㳻ⲭѻ䰤൷ᰐᱮ㪇ᒿࡇ⴨լᙗᡆ਼ⓀᙗǄሶ MON 87427 ѝ 㘬ᒿࡇ൘ EST_2016 ઼ NT_2016 ᮠᦞᓃѝ䘋㹼 BLASTnחս⛩䱴䘁Ⲵสഐ㓴ޕᨂ ࠶᷀ˈ൘ NR_2016 ᮠᦞᓃѝ䘋㹼 BLASTx ࠶᷀ˈ൷ᵚਁ⧠ MON 87427 ᨂޕս 㘬สഐ㓴 DNA ޵䜘ᡆ䱴䘁ᆈ൘ਟ㜭㕆⸱ᯠ㳻ⲭӗ⢙Ⲵ ORFˈҏ⋑ᴹ޵Ⓚᙗח⛩ Ⲵ ORF ഐѪ T-DNA Ⲵᨂޕ㘼㻛⹤ൿǄ

36 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 B-Left Border Region OR-ori V P-e35S

I-hsp70 Nsi I1719 Nco I1952 TS-CTP2 CS-rop T-DNA PV-ZMAP1043 Sph I2184 6 8946 bp

OR-ori-pBR322 CS-cp4 epsps

Nsi I3676 aadA T-nos Sph I3986 B-Right Border Region

᧒䪸 Ự⍻ⴞⲴ 䎧࿻ս⛩˄bp˅ 㓸→ս⛩˄bp˅ ᧒䪸ޘ䮯˄~kb˅ 1 T-DNA 1 1200 1.2 2 T-DNA 1150 2150 1.0 3 T-DNA 2100 3550 1.5 4 T-DNA 3500 4192 0.7 5 䍘㋂僘ᷦ 4193 5942 1.8 6 䍘㋂僘ᷦ 5864 7368 1.5 7 䍘㋂僘ᷦ 7290 8946 1.7 മ6. PV-ZMAP1043䍘㋂മ˄⽪ᵲӔ᧒䪸1-7˅ кമѪُࣙҾߌᵶ㧼䖜ॆᯩ⌅ᗇࡠᣇ㥹⭈㟖䖜สഐ⦹㊣ MON 87427 ᰦᡰ˖⌘ ḷ᰾Ҷ䚇ՐݳԦ઼ח⭘㺘䗮䖭փ PV-ZMAP1043 Ⲵ⧟ᖒ䍘㋂മ䉡Ǆ䍘㋂മཆ Southern ᵲӔ࠶᷀ᡰ⭘䲀ࡦᙗ޵࠷䞦ս⛩˄㋇փ˅ˈSouthern ᵲӔ࠶᷀ᡰ⭘᧒䪸˄᧒ Ǆח䪸 1~7˅⴨ሩս㖞ḷҾ⧟ᖒമ޵

37 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

˅㘬สഐ㓴⡷⇥Ⲵ䟽ਐ PCR ࠶᷀˄CBIח४৺ޕമ 7. ⏥ⴆ MON 87427 ᨂ ᢙ໎⡷⇥Ⲵ亴ᵏབྷሿᱮ⽪൘ PCR ӗ⢙ࠍ㜦മлᯩⲴ MON 87427 ᨂޕᒿࡇ㓯 ᖒമкǄ⇿⌣䚃кṧ䟿Ѫ 4~9 lPCRᢙ໎ӗ⢙Ǆ⌣䚃䈤᰾ྲл˖

⌣䚃 ṧ૱᧿䘠 1 1 kb DNA ࠶ᆀ䟿ḷ䇠 2 MON 87427 3 ሩ➗ LH198 × HiII 4 ᰐ DNA ⁑ᶯሩ➗ 5 MON 87427 6 ሩ➗ LH198 × HiII 7 ᰐ DNA ⁑ᶯሩ➗ 8 MON 87427 9 ሩ➗ LH198 × HiII 10 ᰐ DNA ⁑ᶯሩ➗ 11 1 kb DNA Ladder

㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠

38 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

˅㘬⦹㊣สഐ㓴ᒿࡇ˄CBIחᒿࡇ઼ޕ䮯ᨂޘ മ 8. MON 87427

39 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᒿࡇPCRᢙ໎࠶᷀˄CBIחᒿࡇ৺สഐ㓴ᯱޕ䮯ᨂޘ㺘5. MON 87427

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

40 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᒿࡇѝⲴ䚇Ր䈳᧗ݳԦ৺ަᆳᒿࡇ≷ᙫޕ㺘6. MON 87427ᨂ

1368 bp 㓿ᇶ⸱ᆀՈॆⲴṩⱼߌᵶ㧼 CP4 㧼Ṛ aroA ส cp4 epsps 㕆⸱ᒿࡇ ഐ㕆⸱ᒿࡇˈ㕆⸱ CP4 EPSPS 㳻ⲭ˄Barry et ˄2995-4362˅ al., 1992; Padgette et al., 1996˅ 6bp 䰤ᨂᒿࡇ DNA ݻ䲶઼䖭փᶴᔪ䴰㾱 ˄4363-4368˅ nos 3' 253 bp ṩⱼߌᵶ㧼㜝㜲⻡ਸ䞦สഐ˄ ˅ ㄟ䶎䖜ᖅ nos 㓸→ᆀ ४ˈ㓸→䖜ᖅᒦфᕅሬ mRNA Ⲵཊ㚊㞪㤧䞨 ˄4369-4621˅ ॆ˄Bevan et al., 1983˅ 27 bp 䰤ᨂᒿࡇ DNA ݻ䲶઼䖭փᶴᔪ䴰㾱 ˄4622-4648˅ T-DNA T-DNA 36 bp ṩⱼߌᵶ㧼 ਣ䗩⭼ᒿࡇˈ৲о T-DNA ਣ䗩⭼ᒿࡇ 䖜઼〫ᮤਸ˄Depicker et al., 1982; Zambryski ˄4649-4684˅ et al., 1982˅ 1092 bp ս⛩ 3'ㄟ⴨䛫Ⲵ⦹㊣สഐ㓴 DNAޕ㘬ᒿࡇ ᨂחㄟ'3 ˄4685-5776˅

㘬ᒿࡇ˄㿱മ 8˅Ⲵ⴨ᓄս㖞Ǆחᒿࡇ઼ޕᤜਧḷࠪⲴս㖞ሩᓄҾ MON 87427 ᨂ *

41 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᕅ⢙ᔰਁࠪҶ MON 87427ޣ㘬ᒿࡇ˄മ 8˅ˈ䇮䇑⴨חᒿࡇ઼สഐ㓴ޕṩᦞᨂ 䖜ॆփ⢩ᔲᙗⲴᇊᙗ઼ᇊ䟿Ự⍻ᯩ⌅˄䈖㿱ᵜ⭣䈧Җㅜ 171 亥Āㅜޝ䜘࠶⴨ޣ䱴 Ԧ䍴ᯉĀ4. 䖜สഐᙗ⣦৺ӗ⢙ⲴỰ⍻઼䢤ᇊᢰᵟā˅ǄᇊᙗỰ⍻ᯩ⌅Ѫࠍ㜦⭥⌣ PCR ᯩ⌅ˈᇊ䟿Ự⍻ᯩ⌅Ѫᇎᰦᇊ䟿 PCR ᯩ⌅Ǆᇊᙗ઼ᇊ䟿Ự⍻ᯩ⌅ᡰ⭘ᕅ⢙ǃ ᕅ⢙ս㖞ԕ৺ᢙ໎⡷⇥བྷሿྲл˖ ᇊᙗỰ⍻˄CBI˅˖

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

ᇊ䟿Ự⍻˄CBI˅˖

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

മ 9.䖜สഐ⦹㊣ MON 87427 ࠍ㜦 PCR ᇊᙗỰ⍻ᯩ⌅Ⲵ⢩ᔲᙗ傼䇱㇝ཤᤷ⽪ PCR ӗ⢙Ⲵ⡷⇥䱴䘁࠶ᆀ䟿ḷ䇠˗ަѝˈA മѪ֯⭘ MON 87427˖⌘ 䖜ॆփ⢩ᔲⲴᇊᙗỰ⍻ᕅ⢙䘋㹼 PCR Ⲵ㔃᷌˗B മѪ֯⭘ hmgAᇊᙗᕅ⢙䘋㹼 PCR Ⲵ㔃᷌Ǆ਴⌣䚃ѝᡰ⭘ DNA ⁑ᶯ᧿䘠㿱л亥㺘ṬǄ

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

42 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2.2.2 ࡖ䲚॰ตⲺཝቅૂࣕ㜳㘬Ⲵ DNA ᒿࡇоཙ❦⦹㊣สഐ㓴⴨਼ˈᒦחޕѪҶ⺞ᇊ MON 87427 ѝᨂ 䢤ᇊᑨ㿴ሩ➗ LH198 × HiII สഐ㓴ѝ MON 87427 Ⲵᨂޕս⛩ˈሩ MON 87427 ઼ᑨ㿴ሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA 䘋㹼 PCR ࠶᷀Ǆṩᦞ MON 87427 ᨂޕ 㘬ᒿࡇкˈᕅח㘬ᒿࡇ䇮䇑аሩᕅ⢙ˈަѝᕅ⢙ A սҾ 5'ㄟחєㄟ⴨䛫Ⲵ⇥⡷ 㘬ᒿࡇкˈሩᑨ㿴ਇփሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA 䘋㹼חB սҾ 3'ㄟ ⢙ PCR ᢙ໎࠶᷀˄മ 10˅Ǆ❦ਾሩ PCR ᢙ໎ӗ⢙䘋㹼⍻ᒿˈᒦо MON 87427 ᨂޕ 㘬ᒿࡇ˄മ 8˅䘋㹼∄ሩˈਁ⧠ਇփ૱⿽สഐ㓴ѝᴹ 140 bpחⲴ 5'ㄟ㠣 3'ㄟ⇥⡷ Ⲵᒿࡇ⋑ᴹࠪ⧠൘ MON 87427 Ⲵสഐ㓴 DNA ѝˈਟ㜭ᱟ൘ T-DNA ᮤਸᰦਁ⭏ Ҷࡐ䲔ǄⴞࡽሩҾཆⓀสഐᨂޕᰦᕅ䎧สഐ㓴ᒿࡇ䟽㓴Ⲵᵪࡦ䘈нॱ࠶␵ᾊˈᖸ ᴹਟ㜭ᱟ⭡Ҿ DNA ᮤਸᰦৼ䬮ᯝ㻲؞༽ᵪࡦᕅ䎧˄Salomon and Puchta, 1998˅Ǆ 㻛ࡐ䲔Ⲵ 140 bp ᒿࡇྲл˖

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

43 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

മ10. MON 87427ਇփᑨ㿴⦹㊣Ⲵᨂޕս⛩PCR࠶᷀˄CBI˅

44 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2.2.3 ⴤⲺะഖⲺṮ㤭䞮ᓅࡍૂ᧞ሲⲺ≞ะ䞮ᓅࡍ MON 87427 ⲴᨂޕᒿࡇབྷሿѪ 3746 bpˈ㘼 T-DNA ᨂޕᒿࡇབྷሿѪ 3681 bpǄ 䲔 T-DNA ཆˈ൘T-DNAⲴ5'ㄟ઼ 3'ㄟ࠶࡛ᴹ 41 bp઼ 24 bp Ⲵᨂޕˈ䘉ᱟ൘ T-DNA ᨂޕ䗷〻ѝ㠚㹼ᨂޕⲴᒿࡇǄT-DNA ѝⲴⴞⲴสഐ cp4 epsps ⲴབྷሿѪ 1368 bpǄ cp4 epsps ⲴṨ㤧䞨ᒿࡇ㿱മ ˈ⴨ᖃҾമ 8˄MON 87427 ޘ䮯ᨂޕᒿࡇ઼สഐ 㘬 DNA ᒿࡇ˅ᡰ⽪Ⲵ 2995~4362 bp Ⲵս㖞Ǆח㓴 MON 87427 㺘䗮Ⲵ CP4 EPSPS 㳻ⲭབྷሿ㓖Ѫ 47.6 kDˈਜ਼ᴹ 455 њ≘ส䞨ˈ ṩᦞⴞⲴสഐṨ㤧䞨ᒿࡇ᧘ሬⲴ CP4 EPSPS 㳻ⲭⲴ≘ส䞨ᒿࡇ㿱മ 12Ǆ

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

മ11. MON 87427ѝcp4 epspsสഐⲴṨ㤧䞨ᒿࡇ˄CBI˅ 1 MLHGASSRPA TARKSSGLSG TVRIPGDKSI SHRSFMFGGL ASGETRITGL 51 LEGEDVINTG KAMQAMGARI RKEGDTWIID GVGNGGLLAP EAPLDFGNAA 101 TGCRLTMGLV GVYDFDSTFI GDASLTKRPM GRVLNPLREM GVQVKSEDGD 151 RLPVTLRGPK TPTPITYRVP MASAQVKSAV LLAGLNTPGI TTVIEPIMTR 201 DHTEKMLQGF GANLTVETDA DGVRTIRLEG RGKLTGQVID VPGDPSSTAF 251 PLVAALLVPG SDVTILNVLM NPTRTGLILT LQEMGADIEV INPRLAGGED 301 VADLRVRSST LKGVTVPEDR APSMIDEYPI LAVAAAFAEG ATVMNGLEEL 351 RVKESDRLSA VANGLKLNGV DCDEGETSLV VRGRPDGKGL GNASGAAVAT 401 HLDHRIAMSF LVMGLVSENP VTVDDATMIA TSFPEFMDLM AGLGAKIELS 451 DTKAA മ12. ṩᦞMON 87427ѝcp4 epspsสഐⲴṨ㤧䞨ᒿࡇ᧘ሬⲴ CP4 EPSPS㳻ⲭⲴ≘ส䞨ᒿࡇ 2.2.4 ᨈޛᓅࡍ൞ὃ⢟㓼㜔ѣⲺᇐփδᱥੜ᮪ਾࡦḉ㢨։Ƚਬ㔵։Ƚ 㓵㋈։θᡌԛ䶔᮪ਾᖘᕅᆎ൞ε਀ެ⺤ᇐᯯ⌋ MON 87427 ѝ T-DNA ൘ਇփสഐ㓴Ѫঅᤧ䍍অս⛩ᨂޕˈަ൘ਾԓⲴสഐ 㓴ѝⲴ࠶⿫ҏㅖਸ㓿ިᆏᗧቄ䚇Ր࠶⿫∄˄࠶࡛㿱ㅜ 2.2.5 䜘࠶ᨂޕᒿࡇⲴᤧ䍍 ᮠ࠶઼᷀ㅜ 3.1 䜘࠶ᨂޕっᇊᙗ࠶᷀˅Ǆ࡙⭘৽ੁ PCR ᶕᢙ໎ᨂޕս⛩ཆสഐ 㘬ᒿࡇǄṩᦞᐢ⸕חᒿࡇⲴสഐ㓴ޕ㓴 DNAˈ❦ਾሩᢙ໎⡷⇥⍻ᒿˈԕ⺞ᇊᨂ Ⲵ⦹㊣สഐ㓴ᒿࡇⲴ⢙⨶䘎䬱㗔ˈ࡙⭘ GMAP ㇇⌅˄Wu and Watanabe, 2005˅ˈ 㘬ᒿࡇᇊս൘⦹㊣ㅜ 9 ਧḃ㢢փк䐍ѝᗳ⵰э㋂ 60חᒿࡇⲴޕሶ MON 87427 ᨂ ৈ᪙༴Ǆഐ↔ᴰ㓸ਟԕ⺞ᇊ MON 87427 ѝᨂޕᒿࡇᮤਸࡠ⦹㊣ⲴṨสഐ㓴ѝˈ սҾㅜ 9 ਧḃ㢢փкǄ

45 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2.2.5 ᨈޛᓅࡍⲺ᤭䍓ᮦ ⭘䲀ࡦᙗ޵࠷䞦 Nco I ᡆ Nsi I ⎸ॆ MON 87427 สഐ㓴 DNAˈԕ㜭ཏ⏥ⴆ T-DNA ᒿࡇޘ䮯Ⲵ⴨ӂ䟽ਐ᧒䪸˄᧒䪸 1~᧒䪸 4ˈമ 6 ઼മ 13˅䘋㹼 Southern ᵲӔ࠶᷀ˈӾ㘼⺞ᇊ MON 87427 ѝⲴᨂޕս⛩ᮠ઼ᨂޕᤧ䍍ᮠǄ 㘬ᒿࡇⲴ㓯ᙗ⢙⨶മ䉡˄മ 13˅ˈ⭘䲀ࡦᙗ޵࠷䞦 Nco Iחᒿࡇ৺ަޕṩᦞᨂ 㘬ᒿࡇкᴹ 1 њ䞦חⲴ T-DNA ޵䜘ਚᴹ 1 њ䞦࠷ս⛩ˈᒦ൘⴨䛫Ⲵ 3'ㄟޕ൘ᨂ ࠷ս⛩Ǆྲ᷌ T-DNA ൘ MON 87427 ѝѪঅս⛩অᤧ䍍ᨂޕˈࡉ亴ᵏ⭘ Nco I 䞦 ⎸ॆ MON 87427 สഐ㓴 DNA ਾ㜭ཏӗ⭏ 2 њ䞦࠷⡷⇥ˈަѝањ⡷⇥བྷሿ亴㘬ᒿࡇˈ㘼חᵏѪ~2.2 kbˈवਜ਼ CTP2ǃcp4 epspsǃnosǃਣ䗩⭼ᒿࡇ઼ 3'ㄟ䜘࠶ 㘬ᒿࡇǃᐖ䗩⭼ᒿࡇǃחਖཆањ䗩⭼⡷⇥亴ᵏı2.8 kbˈवਜ਼ᵚ⸕བྷሿⲴ 5'ㄟ e35S ઼ hsp70Ǆ 㘬ᒿח䲀ࡦᙗ޵࠷䞦 Nsi I ൘ T-DNA ޵䜘ᴹ 2 њ䞦࠷ս⛩ˈ൘ަ䛫᧕Ⲵ 5'ㄟ ࡇкᴹ 1 њ䞦࠷ս⛩Ǆྲ᷌ T-DNA ൘ MON 87427 ѝѪঅս⛩অᤧ䍍ᨂޕˈࡉ 亴ᵏ⭘ Nsi I ⎸ॆ MON 87427 สഐ㓴 DNA ਾ㜭ཏӗ⭏ 3 њ䞦࠷⡷⇥Ǆަѝаᶑ ˗㘬ᒿࡇǃᐖ䗩⭼ᒿࡇǃe35S ઼䜘࠶ hsp70חkbˈ䈕ᶑᑖवᤜᐢ⸕བྷሿⲴ 5'ㄟ 1.7~ аᶑᑖ~2.0 kbˈवᤜ䜘࠶ hsp70ǃCTP2ǃcp4 epsps ઼䜘࠶ nos˗ਖཆаᶑᑖı1.3 kbˈ 㘬ᒿࡇǄחवᤜ䜘࠶ nosǃਣ䗩⭼ᒿࡇ઼ᵚ⸕བྷሿⲴ 3'ㄟ MON 87427 ᱟԕ LH198 × HiII ᵲӔ⿽֌Ѫਇփ䖜ॆⲴˈഐ↔䟷⭘ LH198 × HiII ֌Ѫ䱤ᙗሩ➗ˈ਼ᰦ⭘㓿 Sph I 䞦࠷Ⲵ PV-ZMAP1043 䍘㋂ DNA ᡆ ⺞᧒䪸֌Ѫ䱣ᙗሩ➗Ǆ䱣ᙗሩ➗кṧ䟿Ѫ~0.1 ؽ઼~1.0 ؽสഐ㓴 DNA Ⲵ䟿ˈԕ ᇊ Southern ᵲӔⲴ⚥᭿ᓖǄ⭘᧒䪸 1~4˄മ 6 ઼മ 13˅䘋㹼 Southern ᵲӔਟ㜭 Պࠪ⧠ⲴᵲӔᑖර≷ᙫҾ㺘 7ˈᵲӔ㔃᷌㿱മ 14ǃമ 15 ઼മ 16Ǆ ൘ MON 87427 ᨂޕᒿࡇѝнवਜ਼ PV-ZMAP1043 䍘㋂Ⲵԫօ僘ᷦᒿࡇǄ਼ ṧ⭘ Nco I ᡆ Nsi I 䲀ࡦᙗ޵࠷䞦⎸ॆ MON 87427 สഐ㓴 DNAˈᒦ⭘᧒䪸 5~7 䘋㹼ᵲӔਟԕ⺞ᇊ MON 87427 ᱟ੖ਜ਼ᴹ僘ᷦᒿࡇǄ᧒䪸 5~7 ਟԕ㾶ⴆ PV-ZMAP1043 䍘㋂Ⲵޘ䜘僘ᷦᒿࡇ˄മ 6 ઼മ 13˅Ǆ⭘᧒䪸 5~7 䘋㹼 Southern ᵲӔਟ㜭Պࠪ⧠ⲴᵲӔᑖර≷ᙫҾ㺘 7ǄSouthern ᵲӔ㔃᷌㿱മ 17Ǆ

46 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘7. MON 87427ⲴSouthernᵲӔѝ䲀ࡦᙗ޵࠷䞦ǃᵲӔ᧒䪸઼亴ᵏ⡷⇥≷ᙫ

Southern ᵲӔ㔃᷌ മ 14 മ 15 മ 16 മ 17 ⭘Ⲵ᧒䪸 ᧒䪸 1 ઼ 4 ᧒䪸 2 ᧒䪸 3 ᧒䪸 5ǃ6 ઼ 7֯ Ự⍻ⴞⲴ ᨂޕ/ᤧ䍍ᮠ ᨂޕ/ᤧ䍍ᮠ ᨂޕ/ᤧ䍍ᮠ 䍘㋂僘ᷦᒿࡇ

᧒䪸䶦ḷᒿࡇ ޵࠷䞦亴ᵏ Southern ᵲӔؑਧ ~7.1 kb ~7.1 kb 䍘㋂ PV-ZMAP1043 Sph I ~7.1 kb ~7.1 kb ~1.8 kb ~1.8 kb ~1.8 kb ~1.2 kb ᧒䪸⁑ᶯ --* ~1.0 kb ~1.5 kb ~1.5 kb ~0.7 kb ~1.7 kb ı 2.8 kb ı 2.8 kb Nco I ~2.2 kb ᰐᵲӔᶑᑖ ~2.2 kb ~2.2 kb MON 87427 ~1.7 kb ~2.0 kb Nsi I ~2.0 kb ~2.0 kb ᰐᵲӔᶑᑖ ~1.7 kb ı 1.3 kb

*㺘⽪ᵚ⭘⴨ᓄ䞦䘋㹼䞦࠷࠶᷀

47 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427

Nsi I 2533 Nsi I 4490 Nco I4931 Nsi I 795 Nco I2766 r1 r1

1 nos - e35S - T

hsp70 CTP2 5776 - - P I TS cp4 epsps - CS Left Border Region - B B-Right Border Region

Probe 1 Probe 2

Probe 3

Probe 4 ~ 2.2 kb Nco I

~ 1.7 kb ~ 2.0 kb Nsi I

㘬ᒿࡇ৺亴ᵏ䞦࠷⡷⇥བྷሿⲴ㓯ᙗ⽪᜿മחᒿࡇǃสഐ㓴ޕമ13. MON 87427ᨂ ᒿࡇлᯩ㇝ཤ㺘⽪䖜ᖅᯩੁˈⴤ䀂㇝ཤ㺘᰾ޕᒿࡇ䚇Ր㓴ᡀݳԦ઼ Southern ᵲӔ࠶᷀ѝⲴ䲀ࡦᙗ䞦࠷ս⛩ˈᨂޕമк䜘㺘⽪ᨂ 㘬ᒿࡇⲴᔰ࿻ս㖞઼ᯩੁǄമл䜘ᡰ⽪Ѫ䲀ࡦᙗ䞦Ⲵ亴ᵏ䞦࠷⡷⇥བྷሿˈᵜ⽪᜿മᒦᵚ᤹∄ֻ㔈ࡦˈഐ↔䚇ՐݳԦⲴח㊣สഐ㓴⦹ ս㖞઼ T-DNA ᧒䪸བྷሿѪ䘁լ٬Ǆ

48 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 1˅᧒䪸 1 ઼᧒䪸 4 ⲴᵲӔ㔃᷌˄ᨂޕ৺ᤧ䍍ᮠỰ⍻˅ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆᰦˈ൘~6.1 kb ઼~4.1 kb ༴ӗ⭏޵ⓀᙗᵲӔᶑᑖ˄മ 14ˈ⌣䚃 1 ઼ 8˅˗㘼⭘ Nsi I ⎸ॆᰦˈ൘~9.8 kb ઼ ⢙kb ༴ӗ⭏޵ⓀᙗᵲӔᶑᑖ˄മ 14ˈ⌣䚃 3 ઼ 10˅Ǆ䘉ӋᵲӔؑਧ൘⍻䈅 4.3~ ᑨ㿴ሩ➗⌣䚃ѝ൷ᆈ൘ˈഐ↔䇔Ѫ䘉Ӌؑਧᱟ޵ⓀᙗⲴ㛼ᲟᵲӔˈᒦ䶎⢩ᔲᙗ઼ DNAǄ䘉Ӌؑਧᖸਟ㜭ᱟ⭡᧒䪸 1 о޵Ⓚ Hsp70 ޕൠ䪸ሩ MON 87427 ѝⲴᨂ ᒿࡇⲴᵲӔᕅ䎧ⲴˈഐѪ䈕᧒䪸ਜ਼ᴹаሿ䜘࠶ Hsp70 ޵ਜ਼ᆀ˄മ ˅Ǆ⭡Ҿ᧒ 䪸 1 ӵ㾶ⴆҶ Hsp70 ޵ਜ਼ᆀѝᖸሿа䜘࠶ᒿࡇˈᵲӔؑਧ⴨ሩ䖳ᕡǄ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо亴ݸ⭘ Sph I ⎸ॆⲴ PV-ZMAP1043 䍘㋂ DNA ␧ਸ˄മ 14ˈ⌣䚃 7˅ˈ䲔к䘠޵ⓀᙗᵲӔᶑᑖཆˈ 䘈൘~7.1 kb ઼~1.8 kb ༴ӗ⭏ 2 ᶑ亴ᵏᶑᑖ˄മ 13ǃ㺘 7˅Ǆ䱣ᙗሩ➗ᵲӔؑਧ ⲴỰࠪˈ㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔǄ MON 87427 สഐ㓴 DNA ԕ Nco I ⎸ॆ˄മ 14ˈ⌣䚃 2 ઼ 9˅ˈ䲔к䘠޵Ⓚ ᙗᵲӔᶑᑖཆˈ䘈൘~5.5 kb ઼~2.2 kb ༴ӗ⭏ 2 ᶑᑖǄަѝˈ~5.5 kb ᶑᑖԓ㺘Ҷ 㘬สഐ㓴 DNAˈㅖਸı2.8 kb Ⲵ亴ᵏ䗩⭼⡷⇥བྷሿ˄മחT-DNA ৺ަ䛫᧕ ޕᨂ 㘬สഐ㓴חDNA Ⲵ 3'ᵛㄟ৺ަ 3'ㄟ䛫᧕Ⲵ ޕ13ǃ㺘 7˅˗~2.2 kb ᶑᑖԓ㺘Ҷᨂ മ 13ǃ㺘 7˅Ǆ˄DNAˈ䈕㔃᷌ㅖਸ~2.2 kb 䗩⭼⡷⇥Ⲵ亴ᵏ٬ MON 87427 สഐ㓴 DNA ԕ Nsi I ⎸ॆ˄മ 14ˈ⌣䚃 4 ઼ 11˅ˈ䲔к䘠޵Ⓚ ᙗᵲӔᶑᑖཆˈ䘈൘~6.4 kbǃ~2.0 kb ઼~1.7 kb ༴ӗ⭏ 3 ᶑᑖǄަѝˈ~1.7 kb ⡷ 㘬สഐ㓴 DNAˈㅖਸ~1.7 kbחⲴ T-DNA 5'ㄟ৺ަ䛫᧕Ⲵаሿ䜘࠶ޕԓ㺘ᨂ⇥ DNA ޵䜘Ⲵа䜘࠶ᒿ ޕമ 13ǃ㺘 7˅˗~2.0 kb ᶑᑖԓ㺘ᨂ˄Ⲵ䗩⭼⡷⇥亴ᵏ٬ ࡇˈㅖਸ~2.0 kb Ⲵ亴ᵏ⡷⇥བྷሿ˄മ 13ǃ㺘 7˅˗~6.4 kb ᶑᑖԓ㺘ᨂޕ T-DNA 㘬สഐ㓴 DNAˈㅖਸı1.3 kb 䗩⭼⡷⇥Ⲵ亴ᵏབྷሿ˄മח'Ⲵ 3'ㄟ৺ަ䛫᧕Ⲵ 3 13ǃ㺘 7˅Ǆ മ 14 ᡰ⽪㔃᷌㺘᰾ˈ᧒䪸 1 ઼᧒䪸 4 ᡰ⏥ⴆⲴᒿࡇӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ MON 87427 ⲴঅњਟỰ⍻สഐᓗѝǄ 2˅᧒䪸 2 ⲴᵲӔ㔃᷌˄ᨂޕ৺ᤧ䍍ᮠỰ⍻˅ ᑨ㿴ሩ➗ LH198 × HiII ઼ MON 87427 Ⲵสഐ㓴 DNA ࠶࡛⭘ Nco I ᡆ Nsi I ⢙മ 15˅ˈ൷ӗ⭏ҶаӋ޵ⓀᙗᵲӔؑਧˈ䘉ӋᵲӔؑਧ൷ᆈ൘Ҿ⍻䈅˄ॆ⎸ ᑨ㿴ሩ➗⌣䚃ѝ˄മ 15ˈ⌣䚃 1~11˅Ǆഐ↔䇔Ѫ䘉Ӌؑਧᱟ޵ⓀᙗⲴ㛼Ჟᵲ઼ DNAǄ䘉Ӌؑਧᖸਟ㜭ᱟ⭡᧒䪸 2 ޕӔˈᒦ䶎⢩ᔲᙗൠ䪸ሩ MON 87427 ѝⲴᨂ о޵Ⓚ hsp70 ᒿࡇⲴᵲӔᕅ䎧ⲴˈഐѪ䈕᧒䪸ਜ਼ᴹᖸབྷа䜘࠶ hsp70 ޵ਜ਼ᆀᒿࡇ ˄മ 6ǃമ 13˅Ǆ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆ˄മ 15ˈ⌣䚃 1 ઼ 8˅ˈ ൘~4.1 kb ༴ӗ⭏޵ⓀᙗᵲӔᶑᑖ˗ԕ Nsi I ⎸ॆ˄മ 15ˈ⌣䚃 3 ઼ 10˅ˈ൘~5.2 kb ઼~4.2 kb ༴ӗ⭏޵ⓀᙗᵲӔᶑᑖǄ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘

49 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ㋂ӗ⭏Ⲵ᧒䪸⁑ᶯ␧ਸ˄മ 6ˈ᧒ 䪸 2˅ˈ䲔к䘠޵ⓀᙗᵲӔᶑᑖཆˈ䘈൘~1.0 kb ༴ӗ⭏亴ᵏᶑᑖ˄മ 15ˈ⌣䚃 5 ઼ 6˅Ǆᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо亴ݸ⭘ Sph I ⎸ॆⲴ PV-ZMAP1043 䍘㋂ DNA ␧ਸ˄മ 15ˈ⌣䚃䲔к䘠޵ⓀᙗᵲӔᶑᑖཆˈ䘈൘~7.1 kb ༴ӗ⭏亴ᵏᶑᑖǄ䱣ᙗሩ➗ᵲӔؑˈ˅7 ਧⲴỰࠪ㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔǄ MON 87427 สഐ㓴 DNA ԕ Nco I ⎸ॆ˄മ 15ˈ⌣䚃 2 ઼ 9˅ˈ䲔к䘠޵Ⓚ ᙗᵲӔᶑᑖཆˈ䘈൘~5.5 kb ઼~2.2 kb ༴ӗ⭏ 2 ᶑᑖǄަѝˈ~5.5 kb ᶑᑖԓ㺘Ҷ 㘬สഐ㓴 DNAˈㅖਸı2.8 kb Ⲵ亴ᵏ䗩⭼⡷⇥བྷሿ˄മחT-DNA ৺ަ䛫᧕ ޕᨂ 㘬สഐ㓴חDNA Ⲵ 3'ᵛㄟ৺ަ 3'ㄟ䛫᧕Ⲵ ޕ13ǃ㺘 7˅˗~2.2 kb ᶑᑖԓ㺘Ҷᨂ മ 13ǃ㺘 7˅Ǆ˄DNAˈ䈕㔃᷌ㅖਸ~2.2 kb 䗩⭼⡷⇥Ⲵ亴ᵏ٬ MON 87427 สഐ㓴 DNA ԕ Nsi I ⎸ॆ˄മ 15ˈ⌣䚃 4 ઼ 11˅ˈ䲔к䘠޵Ⓚ ᙗᵲӔᶑᑖཆˈ䘈൘~2.0 kb ઼~1.7 kb ༴ӗ⭏ 2 ᶑᑖǄަѝˈ~1.7 kb ⡷⇥ᨂޕ ⇥⡷㘬สഐ㓴 DNAˈㅖਸ~1.7 kb Ⲵ䗩⭼חT-DNA Ⲵ 5'ᵛㄟ઼аሿ䜘࠶䛫᧕Ⲵ- DNA Ⲵ޵䜘ᒿࡇˈㅖਸ~2.0 kb ޕമ 13ǃ㺘 7˅˗~2.0 kb ᶑᑖਜ਼ᴹᨂ˄亴ᵏ٬ Ⲵ亴ᵏ⡷⇥བྷሿ˄മ 13ǃ㺘 7˅Ǆ മ 15 ᡰ⽪㔃᷌㺘᰾ˈ᧒䪸 2 ᡰ⏥ⴆⲴᒿࡇӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ MON 87427 ⲴঅњਟỰ⍻สഐᓗѝǄ 3˅᧒䪸 3 ⲴᵲӔ㔃᷌˄ᨂޕ৺ᤧ䍍ᮠỰ⍻˅ ᑨ㿴ሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA ⭘ Nco I˄മ 16ˈ⌣ 䚃 1 ઼ 8˅ᡆ Nsi I ˄മ 16ˈ⌣䚃 3 ઼ 10˅࠶࡛⎸ॆˈྲ亴ᵏᡰᯉˈ䈕䱤ᙗሩ➗⋑ᴹӗ⭏ਟỰ⍻Ⲵ ᵲӔᶑᑖǄ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘㋂ӗ⭏Ⲵ᧒䪸⁑ᶯ␧ਸ˄മ 6ˈ᧒䪸 3˅ˈ൘~1.5 kb ༴ӗ⭏亴ᵏᶑᑖ˄മ 16ˈ⌣ 䚃 5 ઼ 6˅Ǆᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо亴ݸ⭘ Sph I ⎸ॆⲴ PV-ZMAP1043 䍘㋂ DNA ␧ਸ˄മ 16ˈ⌣䚃 7˅ˈ൘~1.8 kb ༴ӗ⭏亴ᵏ ᶑᑖ˄മ 13ǃ㺘 7˅Ǆᶕ㠚Ҿ䍘㋂Ⲵਖа Sph I ⡷⇥˄~7.1 kb˅ਜ਼ᴹ᧒䪸 3 ᒿࡇ Ⲵаሿ䜘࠶ˈн䗷൘ᵜ⹄ウѝⲴᵲӔ৽ᓄᶑᑖлᵚ㜭ӗ⭏ਟỰ⍻ⲴᵲӔؑਧǄ䱣 ᙗሩ➗ᵲӔؑਧⲴỰࠪ㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔǄ MON 87427 สഐ㓴 DNA ԕ Nco I ⎸ॆ˄മ 16ˈ⌣䚃 2 ઼ 9˅ˈ൘~2.2 kb ༴ 㘬สഐחDNA Ⲵ 3'ᵛㄟ৺ަ 3'ㄟ䛫᧕Ⲵ ޕӗ⭏ 1 ᶑᑖǄ䈕~2.2 kb ᶑᑖԓ㺘Ҷᨂ മ 13ǃ㺘 7˅ǄMON 87427˄㓴 DNA˗䈕㔃᷌ㅖਸ~2.2 kb 䗩⭼⡷⇥Ⲵ亴ᵏ٬ สഐ㓴 DNA ԕ Nsi I ⎸ॆ˄മ 16ˈ⌣䚃 4 ઼ 11˅ˈ൘~2.0 kb ༴ӗ⭏ 1 ᶑᑖˈ↓ ྲ亴ᵏⲴаṧ˄മ 13ǃ㺘 7˅Ǆ䈕~2.0 kb ᶑᑖਜ਼ᴹᨂޕ DNA Ⲵ޵䜘ᒿࡇǄ മ 16 ᡰ⽪㔃᷌㺘᰾ˈ᧒䪸 3 ᡰ⏥ⴆⲴᒿࡇӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ MON 87427 ⲴঅњਟỰ⍻สഐᓗѝǄ 4˅᧒䪸 5ǃ᧒䪸 6 ઼᧒䪸 7 ⲴᵲӔ㔃᷌˄䍘㋂僘ᷦᒿࡇỰ⍻˅ ᑨ㿴ሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA ⭘ Nco ˄മI 17ˈ⌣䚃 1 ઼ 10˅ᡆ Nsi I ˄മ 17ˈ⌣䚃 3 ઼ 12˅࠶࡛⎸ॆˈ⭘ਟԕ㾶ⴆ PV-ZMAP1043 䍘㋂僘ᷦᒿࡇⲴ

50 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᧒䪸 5ǃ᧒䪸 6 ઼᧒䪸 7 ਼ᰦᵲӔˈ↓ྲ䱤ᙗሩ➗亴ᵏᓄᴹ㔃᷌аṧˈ⋑ᴹӗ⭏ ਟỰ⍻ⲴᵲӔᶑᑖǄᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо㓿 Sph I ⎸ॆⲴ PV-ZMAP1043 䍘㋂␧ਸˈ൘~7.1 kb ༴ӗ⭏亴ᵏᶑᑖ˄മ 17ˈ⌣䚃 9˗ 㺘 7˅Ǆᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘㋂ӗ⭏Ⲵ᧒䪸 5 ઼᧒䪸 6˄മ 6˅Ⲵ⁑ᶯ␧ਸˈ൘~1.8 kb ઼~1.5 kb ༴ӗ⭏ 2 ᶑ亴ᵏᶑᑖ˄മ 17ˈ⌣䚃 5 ઼ 6˗㺘 7˅Ǆᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘㋂ӗ⭏Ⲵ᧒䪸 7˄മ 6˅Ⲵ⁑ᶯ␧ਸˈ൘ kb ༴ӗ⭏亴ᵏᶑᑖ˄മ 17ˈ⌣䚃 7 ઼ 8˗㺘 7˅Ǆ䘉Ӌ䱣ᙗሩ➗ᵲӔؑਧⲴ 1.7~ Ựࠪ㔃᷌㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔǄ ᑨ㿴ሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA ⭘ Nco ˄മI 17ˈ⌣䚃 2 ઼ 11˅ᡆ Nsi I ˄മ 17ˈ⌣䚃 4 ઼ 13˅࠶࡛⎸ॆˈ⭘ਟԕ㾶ⴆ PV-ZMAP1043 䍘㋂僘ᷦᒿࡇⲴ ᧒䪸 5ǃ᧒䪸 6 ઼᧒䪸 7 ਼ᰦᵲӔˈ↓ྲ亴ᵏᓄᴹ㔃᷌аṧ˄㺘 7˅ˈ⋑ᴹԫօ ᵲӔᶑᑖࠪ⧠Ǆ䘉а㔃᷌㺘᰾ˈMON 87427 нਜ਼ᴹԫօ PV-ZMAP1043 䍘㋂Ⲵ 僘ᷦᒿࡇǄ

51 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ14. MON 87427ѝT-DNAᨂޕս⛩઼ᤧ䍍ᮠⲴSouthernঠ䘩˄᧒䪸1઼᧒䪸4˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢Ǆ᧒䪸 1 ઼᧒䪸 4 ӵ㾶ⴆ䜘࠶ T-DNA˄മ 6 ઼മ 13˅Ǆ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ޵࠷䞦⎸ॆⲴ DNA ṧ૱Ǆ⌣䚃䈤᰾ྲ л˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ [᧒䪸 1 ઼᧒䪸 4 [~0.1 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 5 [᧒䪸 1 ઼᧒䪸 4 [~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 6 [䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 7 8 ሩ➗ LH198 × HiII˄Nco I˅ 9 MON 87427˄Nco I˅ 10 ሩ➗ LH198 × HiII˄Nsi I˅ 11 MON 87427˄Nsi I˅

㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠 52 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ15. MON 87427ѝT-DNAᨂޕս⛩઼ᤧ䍍ᮠⲴSouthernঠ䘩˄᧒䪸2˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢Ǆ᧒䪸 2 ӵ㾶ⴆ䜘࠶ T-DNA˄മ 6 ઼മ 13˅Ǆ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ޵࠷䞦⎸ॆⲴ DNA ṧ૱Ǆ⌣䚃䈤᰾ྲл˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ [᧒䪸 2 [~0.1 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 5 [᧒䪸 2 [~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 6 [䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 7 8 ሩ➗ LH198 × HiII˄Nco I˅ 9 MON 87427˄Nco I˅ 10 ሩ➗ LH198 × HiII˄Nsi I˅ 11 MON 87427˄Nsi I˅

㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠 53 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ16. MON 87427ѝT-DNAᨂޕս⛩઼ᤧ䍍ᮠⲴSouthernঠ䘩˄᧒䪸3˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢Ǆ᧒䪸 3 ӵ㾶ⴆ䜘࠶ T-DNA˄മ 6 ઼മ 13˅Ǆ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ޵࠷䞦⎸ॆⲴ DNA ṧ૱Ǆ⌣䚃䈤᰾ྲл˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ [᧒䪸 3 [~0.1 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 5 [᧒䪸 3 [~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 6 [䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 7 8 ሩ➗ LH198 × HiII˄Nco I˅ 9 MON 87427˄Nco I˅ 10 ሩ➗ LH198 × HiII˄Nsi I˅ 11 MON 87427˄Nsi I˅

㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠

54 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ17. MON 8742нਜ਼䍘㋂僘ᷦᒿࡇⲴSouthernᵲӔ࠶᷀˄᧒䪸5~7˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢Ǆ᧒䪸 5ǃ᧒ 䪸 6 ઼᧒䪸 7 ਟԕ㾶ⴆޘ䜘 PV-ZMAP1043 䍘㋂Ⲵ僘ᷦᒿࡇ˄മ 6˅Ǆ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ޵࠷䞦⎸ॆ Ⲵ DNA ṧ૱Ǆ⌣䚃䈤᰾ྲл˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 5 ઼᧒䪸 6[~0.1ؽสഐ㓴ᖃ䟿]␧ਸ 5 ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 5 ઼᧒䪸 6[~1.0ؽสഐ㓴ᖃ䟿]␧ਸ 6 ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 7 [~0.1 ؽสഐ㓴ᖃ䟿]␧ਸ 7 ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 7 [~1.0 ؽสഐ㓴ᖃ䟿]␧ਸ 8 ሩ➗ LH198 × HiII˄Nco I˅䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿]␧ਸ 9 10 ሩ➗ LH198 × HiII˄Nco I˅ 11 MON 87427˄Nco I˅ 12 ሩ➗ LH198 × HiII˄Nsi I˅ 13 MON 87427˄Nsi I˅ ㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠

55 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2.3 ⴤⲺะഖф䖳։ᶺᔰഴ䉧θ䖳։Ⲻ੃〦ȽᶛⓆȽ㔉ᶺȽ⢯ᙝૂ ᆿޞᙝθऻᤢ䖳։ᱥੜᴿ㠪⯻ᙝԛ਀ᱥੜਥ㜳╊਎Ѱᴿ㠪⯻ᙝ MON 87427 Ⲵ䖜ॆ䖭փѪ PV-ZMAP1043 䍘㋂ǄPV-ZMAP1043 䍘㋂Ⲵབྷሿ 㓖Ѫ 8.9 kbˈवਜ਼࠶࡛⭡ᐖǃਣ䗩⭼࠶ᔰⲴ T-DNAǄT-DNA वਜ਼ањᆼᮤⲴ cp4 epsps สഐ㺘䗮ⴂˈ䈕สഐ㺘䗮ⴂवᤜ e35S ੟ࣘᆀǃhsp70 Ⲵ޵ਜ਼ᆀǃCTP2 ਦ㔯փ䖜〫㛭ᒿࡇǃcp4 epsps 㕆⸱ᒿࡇ઼ nos 㓸→ᆀǄPV-ZMAP1043 䍘㋂ᡰव 䜘䚇ՐݳԦ≷ᙫҾ㺘 8ˈަ䍘㋂മ䉡㿱മ 6Ǆޘਜ਼Ⲵ PV-ZMAP1043 䍘㋂ᱟӪᐕᶴᔪਸᡀⲴˈަ僘ᷦᶕⓀҾབྷ㛐ᵶ㧼Ǆབྷ㛐ᵶ㧼 ൘⧟ຳ䟼ᰐᡰн൘ˈҏᆈ൘ҾӪ㊫ㅹ㜺Ἶࣘ⢙Ⲵ⎸ॆ㌫㔏ѝ˄Jefferson et al., 1986˅Ǆ⭡Ҿབྷ㛐ᵶ㧼Ⲵ䘉⿽⭏⢙ᆖ⢩ᙗˈа㡜䇔Ѫ⋑ᴹᗵ㾱ਖཆ䘋㹼仍ཆᆹޘ ᙗ࠶᷀ᡆᇎ傼Ǆ䈕㺘䗮䖭փнާᴹ仍ཆⲴ㠤⯵ᙗˈҏнਟ㜭╄ਈᡀѪާᴹ㠤⯵ᙗ Ⲵ䖭փˈሩӪ㊫઼ࣘ⢙ᱟᆹޘⲴǄ PV-ZMAP1043 䍘㋂кⲴᐖ䗩⭼ᒿࡇ઼ਣ䗩⭼ᒿࡇᶕⓀҾṩⱼߌᵶ㧼 ˄Agrobacterium tumefaciens˅˄Barker et al., 1983; Depicker et al., 1982; Zambryski et al., 1982˅Ǆ⇿њ䗩⭼ᒿࡇ਴वਜ਼ᴹ 24-25 bp ൘䖜ॆᰦਟԕਁ⭏ DNA ӔᦒⲴ 㕪࡫ս⛩Ǆᐖਣ䗩⭼ᒿࡇሶ T-DNA ઼䍘㋂僘ᷦᒿࡇ࠶ᔰǄ൘ PV-ZMAP1043 䍘㋂Ⲵ僘ᷦᒿࡇ४ฏ޵वᤜᴹ㓶㧼༽ࡦ䎧⛩˄ori V ઼ ori-pBR322˅ǃ㓶㧼䘹ᤙḷ ᤱ൘བྷ㛐ᵶ㧼ѝⲴᤧ䍍ᮠⲴᕅᆀ㳻ⲭ䱫䙿⢙Ⲵ㕆⸱४˄rop˅˄㺘؍઼ 䇠สഐ aadA Ǆн䇪ᱟ䘉ӋսҾ僘ᷦᒿࡇк䚇ՐݳԦ䘈ᱟ僘ᷦкⲴަᆳᒿࡇ൷⋑ᴹᮤਸ䘋˅8 ⦹㊣สഐ㓴˄മ 17˅Ǆ

56 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘8. PV-ZMAP1043䍘㋂ᡰਜ਼䚇ՐݳԦᾲ䘠䍘㋂ ѝ Ⲵ ˅⥞䚇ՐݳԦ ࣏㜭᧿䘠˄৲㘳᮷ ս㖞˄bp˅ T-DNA ṩⱼߌᵶ㧼 T-DNA ᐖ䗩⭼ᒿࡇˈ৲о T-DNA 䖜઼〫ᮤਸ B1-ᐖ䗩⭼ 1-442 ˄Barker et al., 1983˅ 䰤ᨂᒿࡇ 443-483 DNA ݻ䲶઼ᶴᔪ䴰㾱 P2-e35S वਜ਼ᴹ䟽༽໎ᕪ४ฏ˄Kay et al., 1987˅Ⲵ㣡ὠ㨌㣡ਦ⯵∂ 484-1104 ˄CaMV˅35S RNA Ⲵ੟ࣘᆀ˄Odelletal.,1985˅ 䰤ᨂᒿࡇ 1105-1125 DNA ݻ䲶઼ᶴᔪ䴰㾱 ⦹㊣✝◰㳻ⲭ 70 สഐⲴㅜањ޵ਜ਼ᆀ˄Brown and Santino, I3-hsp70 1126-1929 1997˅ 䰤ᨂᒿࡇ 1930-1953 DNA ݻ䲶઼ᶴᔪ䴰㾱㕆⸱ᤏই㣕 EPSPS 䞦ਦ㔯փ䖜〫㛭४ฏⲴสഐ ShkG Ⲵ䶦 TS4-CTP2 1954-2181 ḷᒿࡇ˄Herrmann, 1995; Klee et al., 1987˅ˈᕅሬ CP4 EPSPS 䖜〫ࡠਦ㔯փ 㓿ᇶ⸱ᆀՈॆⲴṩⱼߌᵶ㧼 CP4 㧼Ṛ aroA สഐ㕆⸱ᒿࡇˈ CS5- 2182-3549 㕆⸱ CP4 EPSPS 㳻ⲭ˄Barry et al., 2001; Padgette et al., cp4 epsps 1996˅ 䰤ᨂᒿࡇ 3550-3555 DNA ݻ䲶઼ᶴᔪ䴰㾱 ṩⱼߌᵶ㧼㜝㜲⻡ਸ䞦สഐ˄nos˅3'ㄟ䶎䖜ᖅ४ˈ㓸→䖜 T6-nos 3556-3808 ᖅᒦфᕅሬ mRNA Ⲵཊ㚊㞪㤧䞨ॆ˄Bevan et al., 1983˅ 䰤ᨂᒿࡇ 3809-3835 DNA ݻ䲶઼ᶴᔪ䴰㾱 ṩⱼߌᵶ㧼 T-DNA ᐖ䗩⭼ᒿࡇˈ৲о T-DNA 䖜઼〫ᮤਸ B-ਣ䗩⭼ 3836-4192 ˄Depicker et al., 1982; Zambryski et al., 1982˅ 䍘㋂僘ᷦ DNA 䰤ᨂᒿࡇ 4193-4328 DNA ݻ䲶઼ᶴᔪ䴰㾱䖜ᓗᆀ Tn7 Ⲵ≘ส㤧㊫ᣇ⭏㍐䫍ॆ䞦˄3''(9)-O-Ṩ㤧䞨䖜〫 aadA 4329-5217 䞦˅Ⲵ੟ࣘᆀǃ㕆⸱ᒿࡇ઼ ㄟ䶎䖜ᖅ४˄Fling et al., 1985˅ˈ ਟԕ֯㓶㧼ᣇᇴ᭮㓯㧼㍐઼䬮䴹㍐䰤ᨂᒿࡇ 5218-5747 DNA ݻ䲶઼ᶴᔪ䴰㾱 OR7-ori- pBR322 䍘㋂Ⲵ DNA ༽ࡦ䎧࿻⛩ˈ㔤ᤱབྷ㛐ᵶ㧼˄E. coli˅ 5748-6336 pBR322 ѝ䍘㋂Ⲵ༽ࡦ˄Sutcliffe, 1979˅ 䰤ᨂᒿࡇ 6337-6763 DNA ݻ䲶઼ᶴᔪ䴰㾱 ColE1 䍘㋂ѝᕅᆀ㳻ⲭ䱫䙿⢙Ⲵ㕆⸱ᒿࡇˈ㔤ᤱབྷ㛐ᵶ㧼 CS-rop 6764-6955 ˄E. coli˅ѝ䍘㋂Ⲵᤧ䍍ᮠ˄Giza and Huang, 1989˅ 䰤ᨂᒿࡇ 6956-8463 DNA ݻ䲶઼ᶴᔪ䴰㾱 ᒯᇯѫ㤳ത䍘㋂ RK2 Ⲵ༽ࡦ䎧࿻ս⛩ˈ㔤ᤱߌᵶ㧼 OR-ori V 8464-8860 ˄Agrobacterium˅ѝ䍘㋂༽ࡦ˄Stalker et al., 1981˅ 䰤ᨂᒿࡇ 8861-8946 DNA ݻ䲶઼ᶴᔪ䴰㾱 1 B–䗩⭼ᒿࡇ˗2 P–੟ࣘᆀ˗3 I–޵ਜ਼ᆀ˗4 TS –䖜〫㳻ⲭᒿࡇ˗5 CS –㕆⸱ᒿࡇ˗6 T– 㓸→ᆀ˗7 OR –༽ࡦ৏⛩ 57 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ၻ඘ᄯЌద௕့ْଫխԅᆇॸ 2.4 ։⭕⢟Ⲻ੃〦ב੥ࣞᆆૂ㓾↘ᆆⲺཝቅȽࣕ㜳਀ެ 2.4.1 MON 87427 ѝ cp4 epsps สഐⲴ㺘䗮⭡ e35S ੟ࣘᆀ傡ࣘǄe35S ੟ࣘᆀབྷሿ Ѫ 621 bpǄe35S ੟ࣘᆀᱟवਜ਼ᴹ䟽༽໎ᕪ४ฏ˄Kay et al., 1987˅Ⲵ㣡ὠ㨌㣡ਦ ⯵∂˄CaMV˅35S RNA Ⲵ੟ࣘᆀ˄Odell et al., 1985˅Ǆ35S ੟ࣘᆀਜ਼ᴹ 2 њ㔃ᶴ ฏ˄㔃ᶴฏ A ઼㔃ᶴฏ B˗㘼 e35S 䲔Ҷ㔃ᶴฏ A ԕཆवᤜᴹ 2 њ㔃ᶴฏ Bˈԕ ໎ᕪ傡ࣘ㺘䗮Ⲵ㜭࣋Ǆe35S ੟ࣘᆀⲴ㔃ᶴਟ৲㿱മ 3Ǆ൘ e35S ੟ࣘᆀਾ䶒ᱟа њᶕ㠚Ҿ⦹㊣✝◰㳻ⲭ 70 สഐ˄hsp70˅Ⲵㅜањ޵ਜ਼ᆀǄ䈕 e35S ੟ࣘᆀ-hsp70 ޵ਜ਼ᆀ㓴ਸਟԕ⢩ᔲᙗ᧗ࡦ CP4 EPSPS 㳻ⲭ൘ MON 87427 Ⲵ㩕ޫಘᇈ઼䳼ᙗ⭏ ⇆ಘᇈ㺘䗮ˈ㘼൘䳴ᙗ⭏⇆ಘᇈн㺘䗮ᡆ㺘䗮䟿ᖸቁǄഐ↔ਟԕ௧ᯭ㥹⭈㟖䲔㥹 ࡲᵰ䳴ˈࡋ䙐Ӫᐕ䳴ᙗн㛢ˈ⭘Ҿࡦ⿽઼⿽ᆀ⭏ӗǄ MON 87427 Ⲵ cp4 epsps สഐⲴ㓸→ᆀѪṩⱼߌᵶ㧼㜝㜲⻡ਸ䞦สഐ˄nos˅ 3'ㄟ䶎䖜ᖅ४ˈབྷሿѪ 253 bpˈਟԕ㓸→䖜ᖅᒦфᕅሬ mRNA Ⲵཊ㚊㞪㤧䞨ॆ ˄Bevan et al., 1983˅Ǆ ։⭕⢟Ⲻ੃〦בḽ䇦ะഖૂᣛ઀ะഖⲺཝቅȽࣕ㜳਀ެ 2.4.2 PV-ZMAP1043 䍘㋂Ⲵᨂޕ४ฏ˄T-DNA˅޵нवᤜԫօḷ䇠สഐ઼ᣕ੺ส ഐǄն⭡ҾⴞⲴสഐ cp4 epsps ਟԕ֯䖜ॆ㓶㜎઼䖜สഐἽṚᣇ㥹⭈㟖ˈഐ㘼൘ 䖜ॆ䗷〻ѝਟԕ֌Ѫ䘹ᤙḷ䇠Ǆcp4 epsps สഐབྷሿѪ 1368 bpˈᶕⓀҾ൏༔ṩⱼ ߌᵶ㧼Ⲵ CP4 㧼Ṛ˄Agrobacterium tumefaciens sp.CP4˅Ǆ ։⭕⢟੃בᆹ㺞䗴䈹᧝ᓅࡍⲺ੃〦਀ެᶛⓆδྸӰᐛਾᡆᡌެ 2.4.3 〦ε ४ฏ˄T-DNA˅޵䘈वᤜަᆳаӋ䈳᧗ᒿࡇᡆݳޕPV-ZMAP1043 䍘㋂Ⲵᨂ ԦǄާփ᧿䘠ྲл˖ T-DNA Ⲵᐖਣ䗩⭼ᒿࡇ˖ᐖ䗩⭼ᒿࡇѪབྷሿ 442 bpˈਣ䗩⭼ᒿࡇབྷሿѪ 357 bpǄ䘉єњ䗩⭼ᒿࡇ൷ᶕⓀҾṩⱼߌᵶ㧼 T-DNA Ⲵ䗩⭼ᒿࡇˈ৲о T-DNA 䖜઼〫ᮤਸǄ ⦹㊣✝◰㳻ⲭ hsp70 ޵ਜ਼ᆀ˖བྷሿѪ 804 bpǄᶕⓀҾ⦹㊣✝◰㳻ⲭ 70 สഐ ˄hsp70˅Ⲵㅜањ޵ਜ਼ᆀǄ޵ਜ਼ᆀⲴ֌⭘ᵪࡦⴞࡽ䘈нॱ࠶␵ᾊˈնਟԕ㛟ᇊ Ⲵᱟ޵ਜ਼ᆀՊ䙊䗷࢚᧕ᵪࡦ৲оἽ⢙Ⲵสഐ㺘䗮䈳᧗Ǆ൘ MON 87427 ѝ hsp70 ޵ਜ਼ᆀ઼ e35S ੟ࣘᆀޡ਼䈳᧗ CP4 EPSPS ൘ MON 87427 Ⲵ㩕ޫಘᇈ઼䳼ᙗ⭏ ⇆ಘᇈ㺘䗮ˈ㘼൘䳴ᙗ⭏⇆ಘᇈн㺘䗮Ǆ CTP2 ਦ㔯փ䖜〫㛭㕆⸱ᒿࡇ˖བྷሿѪ 228 bpǄᶕⓀҾᤏই㣕 EPSPS 䞦ਦ㔯 փ䖜〫㛭४ฏⲴสഐ ShkG Ⲵ䶦ḷᒿࡇˈަ㕆⸱Ⲵਦ㔯փ䖜䘀㛭 CTP2 ᕅሬ CP4 EPSPS 㳻ⲭੁਦ㔯փ䖜䘀Ǆ

58 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2.5 䖢ะഖᯯ⌋ MON 87427 ᱟ䙊䗷ߌᵶ㧼ӻሬ䖜ॆਇփ⦹㊣ᒬ㜊㧧ᗇⲴˈ䖜ॆ䖭փѪӪ ᐕᶴᔪⲴ PV-ZMAP1043Ǆ䟷⭘Ⲵ䖜ॆᯩ⌅о Sidorov ઼ Duncan˄2009˅ӻ㓽 Ⲵᯩ⌅⴨਼Ǆ䖜ॆਇփ LH198 × HiII ᱟњᵲӔ⿽ˈަѝ LH198 ާᴹ㢟ྭⲴߌ㢪 ḃ∄䖳᭿ᝏ৸᱃Ҿ࠶ॆ޽⭏ˈᱟᑨ⭘Ⲵ⦹㊣䖜ॆਇփǄץᙗ⣦ˈ㘼 HiII ሩߌᵶ㧼 Ӿ LH198 × HiII ਇփ⦹㊣Ⲵ䳼ょ࢕ਆᒬ㜊ˈоᩪᑖ䖜ॆ䖭փⲴߌᵶ㧼ޡษ ޫˈ❦ਾሶ㓿оߌᵶ㧼ޡษޫⲴᒬ㜊䖜〫㠣ਜ਼ᴹ㥹⭈㟖ǃ㗗㣴䶂䴹㍐઼⼧䞨≒ Ҽ䫐Ⲵㆋ䘹ษޫสк㔗㔝ษޫǄㆋ䘹ษޫสਟԕᣁࡦᵚ䖜ॆ㓶㜎઼↻։ߌᵶ㧼 Ⲵ⭏䮯ˈӾ㘼ሩ䖜ॆ㓶㜎䘋㹼䘹ᤙǄаᰖ൘ㆋ䘹ษޫสᗇࡠ᜸Ք㓴㓷ˈሶ᜸Ք ⧠䘋ṩ઼㤾Ⲵ࠶ॆǄሶᐢ㓿⭏ṩф㺘׳㓴㓷䖜〫㠣࠶ॆษޫส֯ަ㔗㔝⭏䮯ᒦ ᑨⲴ R0 ԓἽṚ〫ṭࡠ൏༔ѝˈ֯䖜ॆἽṚਟԕ䘋а↕⭏䮯ˈᒦ֌䘋а↕䇴ԧǄ↓

⭡Ҿ R0 ԓἽṚᐢ㓿൘ਜ਼ᴹ㥹⭈㟖ⲴษޫสⲴ䘋㹼䗷ㆋ䘹ˈഐ↔ R0 ԓἽṚ ާᴹᣇ㥹⭈㟖Ⲵ⢩ᙗǄԕ LH198 Ѫ䖞എӢᵜˈሩ R0 ԓἽṚ䘋㹼䘎㔝 4 ԓⲴഎ ӔǄഎӔਾԓ㾱⭘㥹⭈㟖˄0.84 kg a.e./ha˅䘋㹼ㆋ䘹઼䇴ԧǄሶᣇ㥹⭈㟖Ⲵഎ ӔਾԓἽṚ㠚Ӕˈԕ㧧ᗇ㓟ਸњփἽṚˈᒦ⭘ᇊ䟿 PCR ᯩ⌅ሩ㓟ਸἽṚ䘋㹼䢤 ᇊǄᴰ㓸ᗇࡠ㺘ර㢟ྭⲴ MON 87427Ǆ

59 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2.6 Ќద༝ॹζӒԅᆇॸ 2.6.1 ᨈޛᓅࡍ㺞䗴Ⲻಞᇎૂ㓺㓽θྸṯȽ㥄ȽਬȽ㣧Ƚ᷒Ƚ〃ᆆㅿ 㓴㓷઼ಘᇈѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭⲴᇊ䟿ᱟ䙊䗷䞦㚄ݽޣMON 87427 ⴨ ⯛˄ELISA˅Ⲵᯩ⌅ᆼᡀⲴǄMON 87427 Ⲵᶀᯉṧ૱ᱟӾ 2008 ᒤ൘㖾ഭ䘋㹼Ⲵ ⭠䰤䈅傼ѝ䟷䳶Ⲵˈޡ 5 њ䈅傼⛩˄࠶࡛սҾ㖾ഭ⦹㊣ѫӗ४Ⲵ䱯㛟㢢ᐎ Jackcon 䜑ǃ⡡㦧ॾᐎⲴ Jefferson 䜑ǃԺ࡙䈪ԺᐎⲴ Stark 䜑ǃঠㅜᆹ㓣ᐎⲴ Parker 䜑ԕ ৺޵ᐳ᣹ᯟ࣐ᐎⲴ York 䜑˅ˈ⇿њ䈅傼⛩Ⲵ䈅傼Ѫ 3 ⅑䟽༽ˈਆṧ൘ᡰᴹ䈅傼 ⛩Ⲵᡰᴹ䟽༽䘋㹼Ǆਆṧ઼࠶᷀Ⲵᶀᯉवᤜн਼⭏䮯䱦⇥䟷䳶Ⲵ MON 87427 㓴㓷ṧ૱㊫රޡ䇑 19 ⿽˖4 њ⭏㛢ᵏⲴਦ⡷˄OSL1~4˅ǃ㊭㋂ǃ㣡㊹ǃ㣡эǃൠ к䜘ᮤṚǃ㤾ਦǃ4 њ⭏㛢ᵏⲴṩ㌫˄OSR1~4˅ǃ≄⭏ṩǃ㺠㘱ṩ㌫ԕ৺ 4 њ ⭏㛢ᵏⲴᮤṚᶀᯉ˄OSWP1~4˅Ǆ ELISA ࠶᷀㔃᷌≷ᙫҾ㺘 9ǄMON 87427 Ⲵн਼㓴㓷઼ಘᇈѝ CP4 EPSPS 940 μg/g dwt 㳻ⲭⲴ㺘䗮䟿ਈॆ㤳ത䖳བྷˈӾᴰሿ٬վҾỰࠪ䲀˄LOD˅ࡠᴰབྷ٬ ᗞݻ/ݻ㓴㓷ᒢ䟽˅Ǆަѝਦ⡷ѝⲴᒣ൷㺘䗮䟿ᴰ儈˄㤳തӾ OSL3 Ⲵ˄ 㤳തӾ OSWP4 Ⲵ˄μg/g dwt ࡠ OSL1 Ⲵ 680 μg/g dwt˅˗ަ⅑ᱟᮤṚᒣ൷٬ 290 μg/g dwt ࡠ OSWP1 Ⲵ 500 μg/g dwt˅Ǆ䲿ਾ׍⅑Ѫṩ㌫˄Ӿ OSR3 Ⲵ 240 73 μg/g dwt ࡠ OSR1 Ⲵ 140 μg/g dwt˅ǃൠк䜘ᮤṚ˄120 μg/g dwt˅ǃ㣡э ˄100 μg/g dwt˅ǃ≄⭏ṩ˄72 μg/g dwt˅ǃ㺠㘱ṩ㌫˄72 μg/g dwt˅ǃ㤾ਦ˄43 μg/g dwt˅ ઼㊭㋂˄4.2 μg/g dwt˅Ǆ ᴹ 6 њ MON 87427 㣡㊹ṧ૱˄ᶕ㠚 3 њ䈅傼⛩˅Ⲵ CP4 EPSPS 㳻ⲭ㺘䗮䟿 վҾỰࠪ䲀˄

60 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘 9. 2008 ᒤ൘㖾ഭ⭠䰤䟷䳶Ⲵ MON 87427 㓴㓷ṧ૱ѝ CP4 EPSPS 㳻ⲭਜ਼䟿 SD˅ LOD/LOQ4˄SD˅ ᒣ൷٬˄㓴㓷㊫ර ਁ㛢ᰦᵏ 1 ᫝⿽ਾⲴཙ ᒣ൷٬ ᮠ˄DAP˅ 㤳ത 㤳ത 2 3 ਦ⡷ OSL1 V2-V5 20-28 100 (21) 680 (170) 0.069/0.137 75 – 140 400 – 940

OSL2 V6-V8 32-46 83 (25) 410 (130) 0.069/0.137 30 –110 130 – 560

OSL3 V10-V12 41-67 61 (19) 290 (74) 0.069/0.137 35 –95 210 – 410

OSL4 VT 54-73 95 (30) 370 (120) 0.069/0.137 17 – 140 70 – 520

㊭㋂ R6 118-182 3.6 (0.73) 4.2 (0.89) 0.16/0.228 2.6 – 5.3 2.8 – 6.2

㣡㊹ 5 ᦸ㊹ᵏ 58-81 < LOD (NA)

㣡э ᦸ㊹ᵏ 58-76 9.4 (0.97) 100 (12) 0.121/0.137 8.1 – 11 90 – 120

ൠк䜘ᮤṚ R5 83-116 38 (14) 120 (48) 0.069/0.137 8.3 – 57 21 – 200

㤾ਦ R6 124-180 14 (6.3) 43 (27) 0.069/0.137 5.9 – 26 13 – 98

ṩ㌫ OSR1 V2-V5 22-28 18 (5.3) 140 (46) 0.033/0.068 8.1 – 27 58 – 210

OSR2 V6-V8 32-46 16 (6.8) 110 (62) 0.033/0.068 8.3 –29 48 – 240

61 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 SD˅ LOD/LOQ4˄SD˅ ᒣ൷٬˄㓴㓷㊫ර ਁ㛢ᰦᵏ 1 ᫝⿽ਾⲴཙ ᒣ൷٬ ᮠ˄DAP˅ 㤳ത 㤳ത 2 3

OSR3 V10-V12 41-67 12 (4.3) 73 (28) 0.033/0.068 4.9 – 19 22 – 110

OSR4 VT 54-73 15 (5.7) 83 (36) 0.033/0.068 5.6 – 23 23 – 140

≄⭏ṩ R5 83-116 15 (5.2) 72 (23) 0.033/0.068 8.6 – 24 39 – 100

㺠㘱ṩ㌫ R6 124-180 16 (8.3) 72 (37) 0.033/0.068 5.9 – 29 26 – 130

ᮤṚ OSWP1 V2-V5 22-28 50 (8.3) 500 (190) 0.069/0.137 37 – 66 310 – 840

OSWP2 V6-V8 32-46 46 (7.6) 360 (42) 0.069/0.137 33 – 58 300 – 420

OSWP3 V10-V12 41-67 43 (7.1) 380 (78) 0.069/0.137 28 – 56 230 – 500

OSWP4 VT 54-73 37 (6.3) 240 (42) 0.069/0.137 23 – 47 160 – 340

1 䟷ṧᰦ⦹㊣ᡰ༴Ⲵਁ㛢ᰦᵏǄ ᴰ-ݻ勌䟽㓴㓷˄fwt˅ѝਜ਼ᴹⲴᗞݻᮠˈᒣ൷٬ǃḷ߶ᐞ˄SD˅઼㤳ത˄ᴰሿ٬⇿ 2 ˈᱟṩᦞᡰᴹ䈅傼⛩Ⲵṧ૱ᮠᦞ䇑㇇ᶕⲴ˄≄⭏ṩṧ૱ n=11ˈ㣡㊹ṧ૱ n=6˅བྷ٬ ަᆳṧ૱ n=14˅Ǆ ݻᒢ䟽㓴㓷˄dwt˅ѝਜ਼ᴹⲴᗞݻᮠˈᒢ䟽٬ᱟṩᦞ勌䟽٬䲔ԕᒢ䟽䖜ᦒ㌫ᮠ㘼ᗇǄ⇿ 3 4 LOQ=ᇊ䟿䲀ˈLOD=Ựࠪ䲀Ǆ 5 MON 87427 㣡㊹ṧ૱Ⲵ CP4 EPSPS 㳻ⲭ≤ᒣᡆ㘵վҾỰࠪ䲀˄LODˈˈn=6˅ˈ ᡆ㘵ӵ⮕儈Ҿᇊ䟿䲀˄LOQˈˈn=6˅Ǆ

62 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2.6.2 ᨈޛᓅࡍ㺞䗴䠅਀ެ࠼᷆ᯯ⌋ ྲ 2.6.1 䜘࠶᧿䘠ⲴˈMON 87427 ਴㓴㓷઼ಘᇈѝ CP4 EPSPS 㳻ⲭⲴ㺘䗮䟿ᱟ䙊䗷 ELISA ᯩ⌅࠶᷀⺞ᇊⲴǄᵜ⹄ウᡰ⭘ ELISA ࠶᷀ᯩ⌅ྲл˖ 1˅ᶀᯉ༴⨶৺㳻ⲭᨀਆ MON 87427 Ⲵ ELISA ࠶᷀ᶀᯉᱟӾ 2008 ᒤ൘㖾ഭ䘋㹼Ⲵ 5 њ䈅傼⛩⭠䰤䈅傼ѝ䟷䳶Ⲵˈ䘉 5 њ䈅傼⛩वᤜ˖䱯㛟㢢ᐎ Jackcon 䜑ǃ⡡㦧ॾᐎⲴ Jefferson 䜑ǃԺ࡙䈪ԺᐎⲴ Stark 䜑ǃঠㅜᆹ㓣ᐎⲴ Parker 䜑ԕ৺޵ᐳ᣹ᯟ࣐ᐎⲴ York 䜑˅Ǆ⇿њ䈅傼⛩Ⲵ䈅傼Ѫ 3 ⅑䟽༽ˈਆṧ൘ᡰᴹ䈅傼⛩Ⲵᡰᴹ䟽༽䘋㹼Ǆਆṧ ᶀᯉवᤜ˖4 њ⭏㛢ᵏⲴਦ⡷˄OSL1~4˅ǃ㊭㋂ǃ㣡㊹ǃ㣡эǃൠк䜘ᮤṚǃ 㤾ਦǃ4 њ⭏㛢ᵏⲴṩ㌫˄OSR1~4˅ǃ≄⭏ṩǃ㺠㘱ṩ㌫ԕ৺ 4 њ⭏㛢ᵏᮤṚ ˄OSWP1~4˅Ǆ ᡰᴹᇎ傼ᶀᯉ䟷䳶ਾˈޘ䜘䘀㠣ᆏኡ䜭ޜਨᙫ䜘Ǆ䲔㣡㊹ཆˈަᆳᶀᯉ㓿 ᆈ൘-80°C ߠ㇡༷⭘Ǆ؍䜘ᇎ傼ᶀᯉޘ䗷⼘⺾༴⨶Ǆ ᡰᴹ䴰㾱ᨀਆ CP4 EPSPS 㳻ⲭⲴ㓴㓷ᶀᯉ≷ᙫҾ㺘 10Ǆަѝ㊭㋂ᶀᯉⲴ ᨀਆ䟷⭘ਜ਼ᴹ L-㔤⭏㍐ C ઼ 10 mM 㝡≗㛶䞨Ⲵ TBA 㕃ߢ⏢˄0.1 M Tris + 0.1 M ® Na2B4O7 • 10H2O + 0.005 M MgCl2 •6H2O + 0.05% (v/v) Tween -20, pH 7.8 + 0.2% (w:v) L-ascorbic acid + 10 mM 㝡≗㛶䞨˅˗㘼ަᆳᶀᯉⲴ㳻ⲭᨀਆ䟷⭘ਜ਼ ᴹ 0.1%⢋㹰␵㳻ⲭ˄BSA˅Ⲵ pH 7.4 ⼧䞨ⴀ㕃ߢ⏢˄0.001 M KH2PO4 + 0.01 M Na2HPO4 •7H2O + 0.137 M NaCl + 0.0027 M KCl + Tween 20 (1× PBST) + 0.1% (w/v) BSAǄާփᨀਆᯩ⌅Ѫ˖ਆࠪ⴨ᓄⲴ㓴㓷ᶀᯉˈ࣐䘲䟿Ⲵᨀਆ㕃ߢ⏢ˈ㓿 ⏑᯻␧ਸಘ˄Harbil Mixer˅⏑᯻༴⨶ˈ⭘㹰␵䗷└㻵㖞˄Fisher Scientific, ᆈҾ-80°C ߠ㇡༷⭘Ǆ؍Pittsburgh, PA˅└ᦹнⓦ䀓⢙ˈሶᨀਆ⏢࠶㻵ᒦ 㺘10. н਼㓴㓷Ⲵ㳻ⲭᨀਆᯩ⌅

㓴㓷㊫ර 㓴㓷:㕃ߢ⏢∄ ᨀਆ㕃ߢ⏢ 1

ਦ⡷ 2 1:100 1× PBST with 0.1% (w/v) BSA

㊭㋂ 1:100 1× TBA with 10 mM DCA

㣡㊹ 1:100 1× PBST with 0.1% (w/v) BSA

㣡э 1:100 1× PBST with 0.1% (w/v) BSA

ṩ㌫ 3 1:50 1× PBST with 0.1% (w/v) BSA

〨⿶ 4 1:100 1× PBST with 0.1% (w/v) BSA

1 ൘⼘⺾ᶀᯉѝ࣐䘲䟿㕃ߢ⏢ˈ⭘ Harbil ⏑᯻␧ਸಘ༴⨶ˈ⭘㹰␵䗷└ಘ䗷└ᨀਆ⏢Ǆ 2 वᤜഋњᰦᵏⲴᶀᯉ˄OSL1ǃOSL2ǃOSL3 ઼ OSL4˅Ǆ 3 वᤜ OSR1ǃOSR2ǃOSR3 ઼ OSR4 ᰦᵏⲴṩԕ৺≄⭏ṩ઼㺠㘱ṩǄ 4 वᤜൠк䜘ᮤṚǃ㤾ਦ઼ޘ⭏㛢ᵏᮤṚᶀᯉ˄OSWP1ǃOSWP2ǃOSWP3 ઼ OSWP4˅Ǆ

63 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2˅CP4 EPSPS 㳻ⲭᣇփ ⭘Ҿ CP4 EPSPS Ⲵ ELISA Ự⍻Ⲵᦅ㧧ᣇփѪሿ啐অݻ䲶ᣇփ 39B6.1˄IgG 2a ਼ර㳻ⲭˈk-䖫䬮˗ᢩਧ 7022111˅Ǆ䈕ᣇփᱟ⭘㳻ⲭ A ⩬㜲㌆ࠍ㜦Ӣ઼ቲḡ Ӿሿ啐㞩≤ѝ㓟ॆᗇࡠⲴǄ⭘࠶ݹݹᓖ⌅⍻ᗇ㓟ॆⲴ IgG ⎃ᓖѪ 2.3 mg/mlǄ 39B6.1 অݻ䲶ᣇփ⭡ Strategic Biosolutions˄Newark, DE˅ࡦ༷Ǆ㓟ॆⲴᣇփᆈ Ҿਜ਼ᴹ 20 mM ⼧䞨䫐ǃ150 mM ≟ॆ䫐઼ 15 ppm Proclin 300˄Sigma-Aldrich, St. 㚄ᴹ HRP Ⲵኡ㖺ᣇ CP4 EPSPSڦLouis, MO˅Ⲵ㕃ߢ⏢˄pH 7.2˅ѝǄỰ⍻䈅ࡲѪ ᣇփˈҏ㻛〠Ѫᣇ-㳻ⲭ 4˄Sigma-Aldrich, ᢩਧ P-5867˅Ǆ 3˅CP4 EPSPS 㳻ⲭⲴ ELISA ࠶᷀

ሶሿ啐ᣇ CP4 EPSPS ᣇփ⭘व㻛㕃ߢ⏢˄15 mM Na2CO3ˈ35 mM NaHCO3 ઼ 150 mM NaClˈpH 9.6˅〰䟺㠣㓸⎃ᓖ 2.0 μg/mlˈപᇊ൘ 96 ᆄᗞᆄᶯкˈ❦ ਾ൘ 4ºC ߠ㇡ѝᆥ㛢 8hԕкǄԕл਴↕䈅傼᫽֌ѻࡽˈ⭘ 1× PBST ߢ⍇ᗞᆄ ᶯǄੁ⇿њᆄѝ࣐ޕ 100 μl CP4 EPSPS 㳻ⲭḷ߶ᡆṧ૱ᨀਆ⏢ˈ37ºC лᆥ㛢 1hǄ ੁ਴ᆄѝ࣐ޕ 100 μl ᣇ CP4 EPSPS 䗷≗ॆ䞦㔃ਸ⢙ˈԕỰ⍻ᦅ㧧ࡠⲴ μl 100 ޕμl TMB ֯ᶯᱮ㢢Ǆ਴ᆄѝ࣐ 100 ޕCP4 EPSPS 㳻ⲭǄੁ਴ᆄѝ࣐ 6MH3PO4 㓸→䞦⍫৽ᓄǄCP4 EPSPS 㳻ⲭⲴᇊ䟿ᱟ䙊䗷൘ 0.456~14.6 ng/ml 㤳ത޵ˈ࡙⭘޵᧘⌅Ӿ㔈ࡦⲴḷ߶ᴢ㓯к䈫ਆǄ 4˅ਜ਼≤䟿࠶᷀ ⢩ᇊⲴ㓴㓷㊫ර൘⇿њ䈅傼⛩ᔪ・൷аⲴ TSSP˄⢩ᇊ㓴㓷䈅⛩ᮠᦞ⊐ˈ tissue-specific site pool˅Ǆ䲔㣡㊹ཆˈ⇿⿽㓴㓷൷㾱ᔪ・ TSSPǄ䲔㣡㊹ཆˈᡰᴹ Ⲵ㓴㓷Ⲵਜ਼≤䟿࠶᷀൷䟷⭘ IR-200 ⒯ᓖ࠶᷀Ԛᶕ࠶᷀˄Denver Instrument Company, Arvada, CO˅Ǆ਴ TSSP Ⲵᒣ൷≤࠶ਜ਼䟿Ӿй⅑䟽༽࠶᷀ѝ䇑㇇㘼ᗇǄ 䙊䗷л䶒Ⲵ䇑㇇ޜᔿᗇࠪ⇿њ TSSP Ⲵᒢ䟽ᦒ㇇㌫ᮠ˄DWCF˅

[TSSP ⒯ᓖ / 100 %ᒢ䟽ᦒ㇇㌫ᮠ =1–[ᒣ൷٬ ᒢ䟽䖜ᦒ㌫ᮠ⭘Ҿሶԕ 㓴㓷勌䟽˄fwt˅ѪঅսⲴ㳻ⲭ≤ᒣ࠶᷀㔃 ᷌䖜ᦒᡀԕ 㓴㓷ᒢ䟽˄dwt˅অսˈ䇑㇇ޜᔿྲл˖ g/g fwt g/g dwt = DWCF 5˅ᮠᦞ࠶᷀ ԕ SPECTRAmax Plus˄Molecular Devices, Sunnyvale, CA˅ᡆ SPECTRAmax Plus 384˄Molecular Devices, Sunnyvale, CA˅ᗞオᶯ࠶ݹݹᓖ䇑ˈሩᡰᴹⲴ CP4 EPSPS Ⲵ ELISA ৽ᓄᒣᶯ࡙⭘ৼ䟽⌒䮯Ự⍻ᯩ⌅䘋㹼࠶᷀Ǆ⍻ᇊᡰᴹ㳻ⲭ ᰦ৲➗൘⌒䮯Ѫ 620~650 nm ᰦⲴ䈫ᮠᶕ⺞ᇊ਼ˈ൘ 450 nm ⌒䮯༴Ⲵݹ੨᭦٬ ᡰᴹ㳻ⲭⲴ⎃ᓖǄ䟷⭘ Molecular Devices SOFTmax PRO GXP Version 5.0.1 䘋㹼 ᮠᦞ䖜ᦒǄṩᦞḷ߶㳻ⲭⲴ⎃ᓖ৺੨ݹ⦷䈫ᮠᤏਸᡀഋ৲ᮠሩᮠᴢ㓯ǄӾḷ߶ ᴢ㓯޵᧘䇑㇇㳻ⲭ⎃ᓖਾˈᗇࡠ㓴㓷ѝ㳻ⲭⲴਜ਼䟿˄ng/ml˅ˈ⭘ṧ૱〰䟺ഐᆀ 㓴㓷/㕃ߢ⏢〰䟺ؽᮠ䖜ᦒᡀԕ #PѪঅսⲴᮠᦞǄ䲔㣡㊹㓴㓷ཆˈᓄ઼

64 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⭘ᒢ䟽ᦒ㇇㌫ᮠሶ㳻ⲭⲴ勌䟽ਜ਼䟿˄#˅䖜ᦒᡀᒢ䟽ਜ਼䟿˄˅Ǆ࡙ ⭘ Microsoft Excel 2007 䖟Ԧ䇑㇇⦹㊣㓴㓷ѝ CP4 EPSPS 㳻ⲭⲴਜ਼䟿≤ᒣǄ ྲ᷌ ELISA Ự⍻㔃᷌䎵ࠪ亴ᵏ㤳തˈࡉሶ䟽ᯠᨀਆ 2 њ㳻ⲭṧ૱ᒦ䘋㹼 Ự⍻㔃᷌↓⺞ਟ䶐Ǆ؍⺞ELISA ࠶᷀ˈԕ MON 87427 н਼㓴㓷઼ಘᇈѝ CP4 EPSPS 㳻ⲭⲴ㺘䗮䟿䈖㿱 2.6.1 ѝ㺘 9Ǆ 2.6.3 ᨈޛᓅࡍ㺞䗴Ⲻどᇐᙝ MON 87427 ᱟ䙊䗷ߌᵶ㧼ӻሬ䖜ॆᯩ⌅ሶ PV-ZMAP1043 䍘㋂Ⲵ T-DNA ሬ ޕࡠਇփ⦹㊣ LH198 × HiII ѝ㧧ᗇⲴǄLH198 × HiII ᱟњᵲӔ⿽ˈަѝӢᵜ LH198 ḃ∄䖳᭿ᝏ৸᱃Ҿ࠶ॆ޽⭏ˈᱟᑨ⭘ץᴹ㢟ྭⲴߌ㢪ᙗ⣦ˈ㘼 HiII ሩߌᵶ㧼ާ Ⲵ⦹㊣䖜ॆਇփǄ㧧ᗇⲴ R0 ԓἽṚ䙊䗷оߌ㢪ᙗ⣦䖳ྭⲴ LH198 എӔཊԓˈᒦ 㓿䘎㔝㠚ᵲˈᴰ㓸㧧ᗇ MON 87427ǄMON 87427 Ⲵ䘹㛢䗷〻৺㛢⿽മ䉡㿱മ 18Ǆ ᨂޕᒿࡇ㺘䗮っᇊᙗ࠶᷀वᤜҶ MON 87427 Ⲵ 5 њцԓᶀᯉ˄മ 18˅˖ LH198 BC3F3 цԓⲴਦ⡷઼ LH198 BC3F4ǃLH198 BC3F6ǃLH198 BC3F7 ઼ ˄LH198 BC3F7 × LH287˅F1 цԓⲴ⿽ᆀ˗ሩ➗ᶀᯉѪਇփ LH198 × HiII Ⲵ⿽ ᆀǄ䴰㾱ᤷࠪⲴᱟˈቭ㇑㠚 R0 ԓᔰ࿻ˈ䖜ॆἽṚ⭘ LH198 䘋㹼ཊԓഎӔˈަ䚇 ྲ∄˄Ҿ LH198ˈն׍❦ਟԕ㛟ᇊަ䚇Ր㛼Ჟѝᴹਟ㜭ਜ਼ᴹ HiIIੁٮՐ㛼Ჟสᵜ ൘ BC3F4 цԓਟ㜭ਜ਼ᴹ 94%Ⲵ LH198 䚇Ր㛼Ჟ઼ 6%Ⲵ HiII 䚇Ր㛼Ჟ˅Ǆ㘳㲁ࡠ ਟ㜭ᆈ൘Ⲵ޵Ⓚ㛼Ჟˈ䘹⭘ਇփ LH198 × HiII ֌Ѫሩ➗Ǆབྷ㛐ᵶ㧼㺘䗮Ⲵ CP4 EPSPS 㳻ⲭ˄2ng˅֌Ѫḷ߶৲➗㳻ⲭǄ ࡙⭘ CP EPSPS 㳻ⲭⲴ Western ঠ䘩࠶᷀ᶕ䇱ᇎ MON 87427 ѝᨂޕᒿࡇ㺘䗮 ӗ⢙൘ཊњцԓѝⲴっᇊᙗˈWestern ࠶᷀㔃᷌㿱മ 19Ǆ↓ྲ亴ᵏ㔃᷌аṧˈ MON 87427 Ⲵཊњцԓᶀᯉ൷ਟỰ⍻ࡠ CP4 EPSPS 㳻ⲭᶑᑖ˄മ 19ˈ⌣䚃 4~8˅Ǆ LH198 BC3F3 цԓਦ⡷ᶀᯉⲴ Western ݽ⯛ؑਧ˄മ 19ˈ⌣ 䚃 4˅᰾ᱮᕪҾަᆳ цԓⲴ⿽ᆀᶀᯉˈ䈤᰾ CP4 EPSPS 㳻ⲭ൘ਦ⡷ѝⲴ㺘䗮䟿᰾ᱮ儈Ҿ⿽ᆀˈ䘉о CP4 EPSPS 㺘䗮䟿Ⲵ ELISA Ự⍻㔃᷌ᱟа㠤Ⲵ˄㺘 9˅Ǆᡰᴹ䘉ӋᶑᑖⲴ䗱〫 ⦷оབྷ㛐ᵶ㧼㺘䗮 CP4 EPSPS 㳻ⲭⲴ䗱〫⦷˄മ 19ˈ⌣䚃 3˅ᱟа㠤Ⲵˈ䇱ᇎ Ҷ Western ঠ䘩ѝỰ⍻ࡠⲴ MON 87427 ਴цԓᶀᯉݽ⯛ᶑᑖ⺞ᇎᱟ CP4 EPSPS 㳻ⲭˈᒦф਴њцԓ⿽ᆀᶀᯉѝ㺘䗮䟿⴨լ˄മ 19ˈ⌣䚃 5~8˅ˈ㘼ᑨ㿴ሩ➗ᶀ ᯉѝ⋑ᴹỰ⍻ࡠ CP4 EPSPS 㳻ⲭ˄മ 19ˈ⌣䚃 10˅Ǆ Western ࠶᷀㔃᷌㺘᰾ˈMON 87427 Ⲵ CP4 EPSPS 㳻ⲭ൘н਼䚇Րцԓ䰤 ਟԕっᇊ㺘䗮Ǆ ṯᦤр䘦䇺ԭθ৸➝ᵢࣔ⌋ㅢॷӂᶗᴿީḽ߼ࡈ࠼ะഖᬃ֒Ⲻ 2.7 ᆿޞ㊱ශ

ྲкᡰ䘠ˈMON 87427 ᡰ䟷⭘Ⲵสഐ᫽֌⋑ᴹ᭩ਈਇփ⭏⢙ⲴᆹޘᙗǄṩ ᦞǉߌъ䖜สഐ⭏⢙ᆹޘ䇴ԧ㇑⨶࣎⌅ǊㅜҼㄐㅜॱҼᶑⲴ⴨ޣࡂ࠶ḷ߶ˈ⭘ Ҿӗ⭏ MON 87427 Ⲵสഐ᫽֌ᯩ⌅ᓄᖃᖂҾ㊫ර 2ˈণĀнᖡ૽ਇփ⭏⢙ᆹޘ ᙗⲴสഐ᫽֌āǄ

65 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䘋㹼୶ъॆ ૱⿽䖜㛢

TI: [RP×LH198 BC3F4] BC0F1

×RP ˅TI: BC1F1 ˄࠶⿫∄ֻ 1:1 ×RP ˅TI: BC2F1 ˄࠶⿫∄ֻ 1:1 ! ˅TI: BC2F2 ˄࠶⿫∄ֻ 3:1

മ18. MON 87427㛢⿽㌫䉡മ Ӿ LH198 BC3F2 ѝ䘹ᤙаṚᣇ㥹⭈㟖ἽṚ䘋а↕㠚Ӕਟԕᗇࡠ LH198 BC3F3 ˈ䘹ᤙ LH198 BC3F3 ѝ㓟ਸփ㠚Ӕᗇࡠ LH198 BC3F4 Ǆ LH198 BC3F4 цԓ⭘Ҿ࠶ᆀ⢩ᖱ⢩ᙗ࠶᷀ˈᒦ⭘֌䘋а↕എӔ䖜㛢ษ㛢ਟ׋୶ъ ॆᓄ⭘Ⲵ૱⿽ǄLH198 BC3F3ǃLH198 BC3F4ǃLH198 BC3F6ǃLH198 BC3F7 ઼[LH198 BC3F7 × LH287] F1 цԓ˄࣐㋇ᱮ⽪˅㻛⭘Ҿཊцԓ䚇Րっᇊᙗ࠶᷀Ǆ [LH198 BC3F7 × LH287] F1 цԓ⭘Ҿ䘋㹼㳻ⲭ㺘䗮䟿ǃᡀ࠶ǃ㺘රᙗ⣦ǃߌ㢪ᙗ ⣦ԕ৺⧟ຳӂ֌ㅹ࠶᷀ǄRP × LH198 BC3F4 ⲴഎӔ઼㠚Ӕਾԓᶀᯉ⭘Ҿᆏᗧቄ 䚇Րᆖ࠶᷀Ǆ˄F#Ѫ㠚Ӕцԓ˗ Ѫ㠚Ӕ˗BC#ѪഎӔцԓǄRP˖䖜㛢䖞എӢᵜǄ˅

66 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ19. MON 87427ཊњцԓᶀᯉѝCP4 EPSPS㳻ⲭⲴWestern࠶᷀ MON 87427 Ⲵ 4 њцԓ⿽ᆀᶀᯉ઼ 1 њцԓਦ⡷ᶀᯉⲴㅹ䟿ᨀਆ⢙ ԕ৺࠶ᆀ䟿ḷ䇠䟷⭘ SDS-PAGE ࠶⿫ˈ❦ਾ⭥䖜〫㠣 PVDF 㟌Ǆሶ㟌о ˖ኡ㖺ᣇ CP4 EPSPS ᣇփᆥ㛢ˈ⭘ॆᆖਁݹ䈅ࡲᱮ㢢Ǆ⌣䚃䈤᰾ྲл

⌣䚃 ᧿䘠 ࠍ㜦кṧ䟿 1 オⲭ -- 2 ࠶ᆀ䟿ḷ䇠˄Magic Marker˅ 0.5 l 3 བྷ㛐ᵶ㧼˄E. coli˅㺘䗮Ⲵ CP4 EPSPS 㳻ⲭ˄2ng˅ 2ng 4 LH198 BC3F3˄ਦ⡷˅ 20 l 5 LH198 BC3F4˄⿽ᆀ˅ 20 l 6 LH198 BC3F6˄⿽ᆀ˅ 20 l 7 LH198 BC3F7˄⿽ᆀ˅ 20 l 8 (LH198 BC3F7 × LH287)F1˄⿽ᆀ˅ 20 l 9 オⲭ -- 10 ᑨ㿴ሩ➗ LH198 × HiII˄⿽ᆀˈᢩਧ 11214241-004˅ 20 l

67 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3. 䖢ะഖὃ⢟ᆿޞᙝ䇺ԭ 3.1 䖢ะഖὃ⢟Ⲻ䚍Ֆどᇐᙝ 1˅н਼цԓ䰤 T-DNA っᇊᙗⲴ Southern ᵲӔ࠶᷀ ޡ䘋㹼Ҷ 5 њцԓᶀᯉ T-DNA ᨂޕっᇊᙗⲴ Southern ᵲӔ࠶᷀Ǆ䘉 5 њ цԓवᤜ LH198 BC3F3ǃLH198 BC3F4ǃLH198 BC3F6ǃLH198 BC3F7 ઼ ˄LH198 BC3F7 × LH287˅F1˄മ 18˅ˈަѝ LH198 BC3F4 ᱟ⭘ᶕ䘋㹼࠶ᆀ⢩ ᖱ⢩ᙗ࠶᷀Ⲵцԓ˄മ 14ǃമ 15ǃമ 16 ઼മ 17˅Ǆ䖜ॆਇփᵲӔ⿽ LH198 × HiII ԕ৺ᵲӔ⿽ LH198 × LH287 ֌Ѫ Southern ᵲӔⲴሩ➗Ǆ䴰㾱ᤷࠪⲴᱟˈቭ㇑㠚 ˈҾ LH198ੁٮR0 ԓᔰ࿻䖜ॆἽṚᔰ࿻⭘ LH198 䘋㹼ཊԓഎӔˈަ䚇Ր㛼Ჟสᵜ ն׍❦ਟԕ㛟ᇊަ䚇Ր㛼Ჟѝᴹਟ㜭ਜ਼ᴹ HiII˄∄ྲ൘ BC3F4 цԓਟ㜭ਜ਼ᴹ 94%Ⲵ LH198 䚇Ր㛼Ჟ઼ 6%Ⲵ HiII 䚇Ր㛼Ჟ˅Ǆ㘳㲁ࡠਟ㜭ᆈ൘Ⲵ޵Ⓚ㛼Ჟˈ 䘹⭘ਇփ LH198 × HiII˄㘼䶎 LH198˅֌Ѫሩ➗Ǆ Ӿ MON 87427 Ⲵн਼цԓᶀᯉԕ৺ሩ➗ᶀᯉᨀਆ DNA˄LH198 BC3F3ǃ LH198 BC3F6 ઼ LH198 BC3F7 Ⲵ DNA ᱟӾਦ⡷ᨀਆˈަᆳцԓ DNA ᱟӾ⿽ ᆀѝᨀਆⲴ˅ˈ㓿䲀ࡦᙗ޵࠷䞦 Nsi I ⎸ॆ˄മ 13˅ˈ⭘ PV-ZMAP1043 䍘㋂Ⲵ ᧒䪸 1 ઼᧒䪸 4˄മ 6˅䘋㹼 Southern ᵲӔǄྲ᷌ T-DNA ᨂޕ൘цԓ䰤н㜭ཏ っᇊ䚇ՐˈࡉՊ൘ Southern ᵲӔ㔃᷌ѝࠪ⧠䶎亴ᵏᶑᑖ˄亴ᵏᶑᑖ㿱മ 13 ઼ 㺘 7˅Ǆ⭘᧒䪸 1 ઼᧒䪸 4 ᵲӔⲴ㔃᷌㿱മ 20Ǆ ᰐ䇪ᱟሩ➗䘈ᱟ MON 87427 䜭൘~4.3 kb ༴Ự⍻ࡠаᶑᵲӔᶑᑖ˄മ 20ˈ 䚃 1~10˅ˈഐ↔ਟԕ䇔Ѫ䈕ᵲӔᶑᑖ኎Ҿ⦹㊣޵Ⓚ㛼ᲟⲴᵲӔؑਧˈ㘼о⌣ MON 87427 Ⲵᨂޕ DNA ᰐޣǄ ሩ➗ LH198 × HiII สഐ㓴 DNA ⭘ Nsi I ⎸ॆਾо᧒䪸⁑ᶯ˄᧒䪸 1 ઼᧒ 䪸 4˅␧ਸˈ䲔~4.3 kb Ⲵ޵ⓀᙗᵲӔᶑᑖཆˈ䘈ӗ⭏ҶⓀҾ䍘㋂ PV-ZMAP1043 Ⲵ~1.2 kb ઼~0.7 kb 亴ᵏབྷሿᶑᑖ˄മ 20ˈ⌣䚃 1 ઼ 2˅Ǆᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ⭘ Nsi I ⎸ॆਾо亴ݸ⭘ Sph I ⎸ॆⲴ䍘㋂䖭փ PV-ZMAP1043 ␧ ਸˈ䲔~4.3 kb Ⲵ޵ⓀᙗᵲӔᶑᑖཆˈ䘈൘~7.1 kb ઼~1.8 kb ༴ӗ⭏ 2 ᶑ亴ᵏབྷ ሿⲴᶑᑖ˄മ 20ˈ⌣ 䚃 3˅Ǆ䱣ᙗሩ➗ⲴỰࠪ㺘᰾᧒䪸оަ䶦ḷᒿࡇਟԕᵲӔǄ Ӿ LH198 BC3F3ǃLH198 BC3F4ǃLH198 BC3F6ǃLH198 BC3F7 ઼˄LH198 BC3F7 × LH287˅F1 цԓᨀਆⲴ DNA ⭘ Nsi I ⎸ॆ˄മ 20ˈ⌣䚃 5ǃ6ǃ7ǃ8 ઼ 10˅ˈ䲔к䘠޵ⓀᙗᵲӔᶑᑖཆˈ䘈൘~6.4 kbǃ~2.0 kb ઼~1.7 kb ༴ӗ⭏ 3 ᶑ亴ᵏᶑᑖ˄㺘 7˅Ǆ~1.7 kb ᶑᑖԓ㺘Ҷᨂޕ T-DNA Ⲵ 5’ᵛㄟԕ৺аሿ䜘࠶ 㘬สഐ㓴 DNAˈㅖਸ~1.7 kb 䗩⭼⡷⇥Ⲵ亴ᵏབྷሿ˗~2.0 kb ᶑᑖਜ਼ᴹח䛫᧕Ⲵ а䜘࠶ᨂޕ DNA ޵䜘ᒿࡇǄ~6.4 kb ᶑᑖԓ㺘 T-DNA Ⲵ 3’ᵛㄟԕ৺ᨂޕ 3’ㄟ㘬䛫᧕Ⲵสഐ㓴 DNAˈㅖਸ@\ 䗩⭼⡷⇥Ⲵ亴ᵏབྷሿǄMON 87427 Ⲵח LH198 BC3F3ǃLH198 BC3F6ǃLH198 BC3F7 ઼˄LH198 BC3F7 × LH287˅F1 цԓ˄മ 20ˈ⌣䚃 5ǃ6ǃ7ǃ8 ઼ 10˅ӗ⭏Ⲵᑖ䉡⢩ᖱо LH198 BC3F4 цԓ⴨ ਼˄മ 14ˈ⌣䚃 4 ઼ 11˅ˈ㺘᰾ MON 87427 ѝঅњᤧ䍍Ⲵ T-DNA ൘ᡰ䘹ц ԓѝਟԕっᇊ䚇ՐǄ 68 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ20. MON 87427ᨂޕᒿࡇ൘ཊцԓ䰤っᇊⲴSouthern࠶᷀˖᧒䪸1઼᧒䪸4

ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢Ǆ᧒䪸 1 ઼᧒䪸 4 ӵ㾶ⴆ䜘࠶ T-DNA˄㿱 ᵜ⭣䈧ҖⲴമ 6 ઼മ 13˅ǄLH198 BC3F3ǃLH198 BC3F6 ઼ LH198 BC3F7 Ⲵ DNA ᱟӾਦ⡷ᨀਆˈަᆳцԓ DNA ᱟӾ⿽ᆀѝᨀਆⲴǄ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ ޵࠷䞦⎸ॆⲴ DNA ṧ૱Ǆ⌣䚃䈤᰾ྲл˄ᤜਧ޵㺘⽪⭘Ҿ⎸ॆⲴ޵࠷䞦˅˖

⌣䚃 ṧ૱᧿䘠 [᧒䪸 1 ઼᧒䪸 4[⴨ᖃҾ~0.1 ؽสഐ㓴 DNAޕሩ➗ LH198 × HiII (Nsi I) ␧ 1 [᧒䪸 1 ઼᧒䪸 4[⴨ᖃҾ~1.0 ؽสഐ㓴 DNAޕሩ➗ LH198 × HiII (Nsi I) ␧ 2 [䞦࠷䍘㋂ (Sph I) [⴨ᖃҾ~1.0 ؽสഐ㓴 DNAޕሩ➗ LH198 × HiII (Nsi I) ␧ 3 4 ሩ➗ LH198 × HiII (Nsi I) 5 MON 87427 (LH198 BC3F3) (Nsi I) 6 MON 87427 (LH198 BC3F4) (Nsi I) 7 MON 87427 (LH198 BC3F6) (Nsi I) 8 MON 87427 (LH198 BC3F7) (Nsi I) 9 ሩ➗ LH198 × LH287 (Nsi I) 10 MON 87427([LH198 BC3F7 × LH287] F1) (Nsi I) ㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠

69 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2˅MON 87427 ሬޕᙗ⣦Ⲵ䚇Ր࠶⿫ ൘ MON 87427 Ⲵ⹄ウᔰਁ䗷〻ѝˈ䘋㹼Ҷ⴨ޣⲴ䚇Ր࠶⿫䈅傼ˈԕ䇴ՠ MON 87427 ѝᨂޕⲴ cp4 epsps สഐ㺘䗮ⴂⲴ䚇ՐっᇊᙗǄ࡙⭘঑ᯩ⍻傼ᶕ∄䖳 ᇎ䱵࠶⿫∄઼ֻ׍ᦞᆏᗧቄ䚇Ր㿴ᖻ䇑㇇Ⲵ亴ᵏ࠶⿫∄ֻⲴᤏਸ〻ᓖǄ ঑ᯩⲴ䇑㇇ޜᔿѪ˖ 02`5|}M ަѝ˖o= ᇎ䱵㿲ሏࡠⲴสഐරᡆ㺘⧠ර仁⦷˗e= ⨶䇪亴ᵏสഐරᡆ㺘⧠ර仁⦷˗ 㔏䇑ᱮ㪇≤ᒣ䇮ᇊѪ `Ǆ

䖜ॆᗇࡠⲴ R0 ԓἽṚ⭘ LH198 എӔࠐԓਟԕᗇࡠᵲਸⲴ LH198 BC3F1 ц ԓǄሶ LH198 BC3F1 㠚Ӕਟԕᗇࡠ࠶⿫㗔փ LH198 BC3F2ǄӾ LH198 BC3F2 ѝ䘹ᤙаṚᣇ㥹⭈㟖ἽṚ䘋а↕㠚Ӕਟԕᗇࡠ LH198 BC3F3 ˈ䘹ᤙ LH198 BC3F3 ѝ㓟ਸփ㠚Ӕᗇࡠ LH198 BC3F4˄മ 18˅ǄLH198 BC3F4 Ⲵ⿽ᆀ ⭘ҾഎӔ䖜㛢ᶕ䘋а↕ᔰਁ୶ъॆⲴ૱⿽Ǆާփᱟ⭘нਜ਼ cp4 epsps สഐ㺘䗮ⴂ ⲴӢᵜ˄〠Ѫ䖞എӢᵜˈRP˅എӔǄഎӔᖃԓѪᵲਸ㗔փˈ〠Ѫ˄RP × LH198 BC3F4˅ BC0F1Ǆ䘋а↕എӔᗇࡠ˄RP × LH198 BC3F4˅BC1F1ˈሩަ௧ᯭ㥹⭈㟖ਟԕ䘹 ᤙᣇ㥹⭈㟖ⲴἽṚǄ⭘ᣇᙗἽṚ޽എӔᗇࡠ BC2F1ˈ൘ BC2F1 цԓ䘹ᤙᣇᙗἽ Ṛ㠚Ӕᗇࡠ BC2F2 ԓǄ൘ BC1F1ǃBC2F1 ઼ BC2F2 цԓ䈳ḕ㥹⭈㟖ᣇᙗἽṚⲴ ࠶⿫∄ֻˈBC1F1 ઼ BC2F1 ⲴᣇᙗἽṚ:᭿ᝏἽṚⲴ⨶䇪࠶⿫∄ֻѪ 1:1ˈ㘼 BC2F2 Ⲵ⨶䇪࠶⿫∄ֻѪ 3:1˄മ 18˅Ǆ䈳ḕⲴἽṚᮠǃ⨶䇪࠶⿫∄ֻ৺ަ঑ᯩ ⍻傼㔃᷌㿱㺘 11ǄӾ䘉Ӌ㔃᷌ਟⴻࠪˈMON 87427 Ⲵᣇ㥹⭈㟖ᙗ⣦ㅖਸᆏᗧቄ অสഐ䚇Ր㿴ᖻǄӾ㘼䇱ᇎ MON 87427 ѝⲴ cp4 epsps สഐ㺘䗮ⴂ൘অаս⛩к ӵᴹঅᤧ䍍ᨂޕˈᒦਟ൘ਾԓѝっᇊ䚇ՐǄ ֻ∄㺘11. MON 87427Ⲵ㓴㓷⢩ᔲᙗ㥹⭈㟖ᣇᙗⲴ䚇Ր࠶⿫

㿲ሏἽṚ 㿲ሏࡠ 㿲ሏࡠ 亴ᵏⲴ 亴ᵏⲴ цԓ 1 2 ᱮ㪇ᙗ ᙫᮠ 2 䱣ᙗṚᮠ 䱤ᙗṚᮠ 䱣ᙗṚᮠ 䱤ᙗṚᮠ

BC1F1 238 109 129 119 119 1.6807 >0.05

BC2F1 290 145 145 145 145 0 >0.05

BC2F2 1107 820 287 830 277 0.5062 >0.05

1 ᡰ䘹⭘цԓᶀᯉⲴ䉡㌫ޣ㌫䈧৲㿱മ 18˗ 2 䇴ԧⲴἽṚᙫᮠˈަѝ䱣ᙗṚѪ㥹⭈㟖㙀ਇරἽṚˈ䱤ᙗṚѪ㥹⭈㟖᭿ᝏරἽṚǄ 3˅MON 87427 н਼цԓᶀᯉ CP4 EPSPS 㺘䗮Ⲵ Western ࠶᷀ ࡽ 2.6.3 䜘࠶ᡰ䘠ˈ֯⭘ Western ᯩ⌅࠶᷀ MON 87427 㛢⿽䉡㌫Ⲵ 5 њцྲ ԓᶀᯉˈवᤜ˖LH198 BC3F3 цԓⲴਦ⡷઼ LH198 BC3F4ǃLH198 BC3F6ǃ LH198 BC3F7 ઼˄LH198 BC3F7 × LH287˅F1 цԓⲴ⿽ᆀ˗ሩ➗ᶀᯉѪਇփ LH198 × HiII Ⲵ⿽ᆀǄ࠶᷀㔃᷌㺘᰾ CP4 EPSPS 㳻ⲭ൘ MON 87427 㛢⿽䉡㌫Ⲵ 5 њцԓᶀᯉѝ൷ਟԕっᇊ㺘䗮˄മ 19˅Ǆ 70 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.2 䖢ะഖὃ⢟ф਍։ᡌӨᵢὃ⢟൞⧥ູᆿޞᙝᯯ䶘Ⲻᐤᔸ 3.2.1 ⭕⇌ᯯᕅૂ⭕⇌⦽ ൘ 2008 ᒤ㖾ഭ 16 њൠ⛩Ⲵ⭠䰤䈅傼ѝ䈳ḕҶ 14 њоἽṚ⭏䮯઼ਁ㛢ᴹ ޣⲴᙗ⣦ˈ䘉Ӌ䈅傼ൠ⛩สᵜਟԕ㾶ⴆ㖾ഭⲴѫ㾱⦹㊣ӗ४Ⲵ⭏䮯ᶑԦǄ ᙫҾ㺘 12Ǆ≶MON 87427 ઼ሩ➗Ⲵ 14 њᙗ⣦൘ 16 њ䈅傼ൠ⛩Ⲵᒣ൷٬ 㺘12. 2008ᒤ㖾ഭ⭠䰤䈅傼ѝMON 87427৺ަሩ➗Ⲵ㺘⧠රᙗ⣦Ⲵᒣ൷٬

S.E.˅ ৲㘳૱⿽ᮠ٬㤳ത1˄ᒣ൷٬ 㺘⧠රᙗ⣦˄অս˅ ᴰབྷ٬ MON 87427 ሩ➗ ᴰሿ٬ ᒬ㤇⭏⍫࣋˄1-9㓗˅ 2.7 (0.19)* 2.4 (0.17) 1.0 5.0 ᰙᵏᒬ㤇ᮠ˄Ṛ/ሿ४˅ 67.7 (1.69) 70.3 (2.01) 55.7 80.3 50%ἽṚᮓ㊹ཙᮠ˄d˅ 63.9 (1.15) 63.5 (1.19) 45.7 78.0 50%ἽṚᣭэཙᮠ˄d˅ 62.9 (1.07) 62.7 (1.09) 46.7 75.0 㔯ᙗ˄1-9㓗˅ 5.9 (0.34) 5.6 (0.35) 2.0 9.0؍ ょս儈ᓖ˄in.˅ 42.0 (0.89) 41.9 (0.97) 26.3 56.1 Ṛ儈˄in.˅ 91.4 (1.50) 90.8 (1.50) 73.9 103.1 л඲ょᮠ˄ょ/ሿ४˅ 0.6 (0.22) 0.5 (0.17) 0.0 2.7 ԿṚᮠ˄Ṛ/ሿ४˅ 7.7 (2.58) 5.5 (1.77) 0.0 71.3ق㤾 ԿṚᮠ˄Ṛ/ሿ४˅ 7.5 (2.49) 5.3 (2.08) 0.0 25.9قṩ ᴰ㓸ἽṚᮠ˄Ṛ/ሿ४˅ 60.8 (0.89) 60.4 (0.88) 54.7 65.8 ㊭㋂ਜ਼≤䟿˄%˅ 19.6 (0.55) 20.3 (0.62) 16.0 27.4 ᇩ䟽˄lbs/bu˅ 55.3 (0.33) 55.2 (0.38) 51.6 58.6 ᣈ㇇ӗ䟿˄bu/a˅ 156.9 (5.47) 165.4 (6.71) 94.6 193.8 ⌘˖䈅傼䇮䇑䟷⭘ᆼޘ䲿ᵪ४㓴䇮䇑ˈ3⅑䟽༽ˈS.E. = ḷ߶ᐞˈn=48Ǆ * 㺘᰾⍻䈅૱⿽઼ሩ➗૱⿽䰤ᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲ˄`˅Ǆ 㘳૱⿽Ⲵᮠ٬㤳തᱟ׍ᦞᐢ㓿୶ъॆⲴ 38 њ૱⿽Ⲵᴰሿ઼ᴰབྷ٬ᗇࠪⲴǄ৲ 1 MON 87427 ઼䶎䖜สഐሩ➗䰤൘䲔Ҷˈṩᦞᡰᴹ䈅傼ൠ⛩Ⲵᙗ⣦ᒣ൷٬ ᒬ㤇⭏⍫࣋ཆⲴᡰᴹަᆳᙗ⣦䰤⋑ᴹ㔏䇑ᆖᱮ㪇ᙗᐞᔲǄMON 87427 ઼ሩ➗䰤 ୟаᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲⲴᙗ⣦ᱟᒬ㤇⭏⍫࣋ǄMON 87427 Ⲵᒬ㤇⭏⍫࣋㓗࡛ ∄ሩ➗儈˄2.7 㓗 vs. 2.4 㓗ˈԕ 1-9 ࠶㓗˅Ǆቭ㇑Ҽ㘵䰤ᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲˈ նᒣ൷ᮠⲴᐞᔲᖸሿˈӵѪ 0.3˄ޡ 9 㓗˅Ǆᒦф MON 87427 Ⲵᒬ㤇⭏⍫࣋㓗 ࡛൘а਼⿽ἽⲴ୶ъॆ৲➗૱⿽Ⲵ㠚❦ਈᔲ㤳ത޵˄ԕ 1-9 ࠶㓗˅Ǆഐ↔ˈ䈕㔏䇑ᆖкⲴᱮ㪇ᐞᔲнާᴹ⭏⢙ᆖ᜿ѹǄ ൘о⭏⇆ᯩᔿ઼⭏⇆⦷⴨ޣⲴᙗ⣦ѝˈྲᮓ㊹઼ᣭэཙᮠǃょս儈ᓖǃṚ Կ઼ӗ䟿ㅹˈMON 87427 оᑨ㿴ሩ➗⋑ᴹᱮ㪇ᙗᐞᔲǄق儈ǃ 2014 ᒤ൘ਹ᷇ⴱߌ、䲒䘋㹼Ⲵ MON 87427 ᣇ㥹⭈㟖⦹㊣⧟ຳᆹޘᙗ䇴ԧ Ⲵ⭠䰤䈳ḕᮠᦞҏ㺘᰾ˈ൘ṭษൠ䈅傼ᶑԦлˈ䖜สഐ⦹㊣ MON87427 ൘Ṛ儈ǃ

71 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㾶ⴆᓖǃӗ䟿ǃਾԓ⿽ᆀਁ㣭⦷ㅹᯩ䶒ˈоሩᓄⲴ䶎䖜สഐ⦹㊣ᰐᱮ㪇ᐞᔲǄ 㔬кᡰ䘠ˈMON 87427 Ⲵ㺘⧠රᙗ⣦˄वᤜо⭏⇆ᯩᔿ઼⭏⇆⦷⴨ޣⲴ ᙗ⣦˅оሩ➗⋑ᴹ⭏⢙ᆖ᜿ѹкⲴᐞᔲˈнՊ໎࣐ަ䖜ਈѪᴹᇣ⭏⢙ᡆሩ⧟ຳ Ǆੁٮӗ⭏н࡙ᖡ૽Ⲵ 3.2.2 Ֆ᫣ᯯᕅૂՖ᫣㜳࣑ ⦹㊣኎Ҿ䳼䳴਼Ṛᔲ㣡ᦸ㊹֌⢙Ǆ᰾⺞ MON 87427 ৺ަᑨ㿴ሩ➗Ⲵ㣡㊹ ᖒᘱ઼㣡㊹⭏⍫࣋ᱟ੖ᆈ൘ᐞᔲਟԕ䇴ԧҼ㘵൘Ր᫝ᯩᔿ઼Ր᫝㜭࣋ᯩ䶒ᱟ੖ ᆈ൘ᐞᔲǄ⭘Ҿ⹄ウⲴ㣡㊹ᱟӾսҾ㖾ഭᇶ㣿䟼ᐎⲴ⭠䰤䈅傼ѝᵚ㓿㥹⭈㟖༴ ⨶Ⲵ MON 87427ǃᑨ㿴ሩ➗ԕ৺ 4 њ୶ъॆⲴ৲➗૱⿽к䟷䳶ⲴǄ⭠䰤䈅傼Ѫ ᆼޘ䲿ᵪ४㓴ᧂࡇˈ3 ⅑䟽༽ˈ⇿ሿ४䲿ᵪ䘹ᤙ 3 Ṛ⭘Ҿ䟷䳶㣡㊹Ǆ㣡㊹⭘ Alexander Ⲵᯩ⌅ḃ㢢˄Alexander, 1980˅Ǆ㣡㊹⍫࣋⹄ウ㠣ቁ⇿њṧᵜ䈳ḕ 100 ㋂㣡㊹ˈ㘼㣡㊹ⴤᖴ⹄ウࡉ൘⇿䟽༽޵⍻䟿 10 ㋂ާᴹԓ㺘ᙗᒦᴹ⍫࣋Ⲵ㣡㊹Ǆ ᇎ傼㔃᷌㺘᰾ˈMON 87427 ઼ሩ➗൘㣡㊹㋂ⴤᖴᯩ䶒⋑ᴹ㔏䇑ᆖᱮ㪇ᙗ ᐞᔲ˄㺘 13˅Ǆṩᦞⴤ㿲ⴞ⍻ˈMON 87427 ઼ሩ➗Ⲵ㣡㊹ᖒᘱ⋑ᴹ᰾ᱮн਼Ǆ ն MON 87427 㣡㊹⭏⍫࣋˄99.7%˅к൘㔏䇑ᆖкᱮ㪇儈Ҿሩ➗˄98.9%˅Ǆቭ ㇑ MON 87427 㣡㊹⍫࣋〽ᗞ儈Ҿ୶ъॆ৲➗૱⿽Ⲵᮠ٬㤳തˈн䇪ᱟ MON 87427 䘈ᱟሩ➗ˈަ㣡㊹⍫࣋Ⲯ࠶ᮠ䜭䶎ᑨ儈ˈᒦфҼ㘵ⲴⲮ࠶ᮠѻᐞᴹ 䶎ᑨሿ˄ਚᴹ 0.8%˅Ǆ䘉ӋᖸሿⲴᐞᔲнཚਟ㜭ާᴹ⭏⢙ᆖ᜿ѹǄഐ↔ˈоᑨ 㿴ሩ➗⴨∄ˈMON 87427 Ⲵ㣡㊹⍫࣋⋑ᴹਁ⭏ᇎ䍘ᙗ᭩ਈˈнՊ໎࣐ ӗ⭏ޘҏнՊሩ⧟ຳᆹˈੁٮMON 87427 Ⲵᵲ㥹ॆᡆ㘵䖜ਈѪަᆳᴹᇣ⭏⢙Ⲵ н࡙ᖡ૽Ǆ 㺘13. MON 87427৺ሩ➗㣡㊹㋂⢩ᖱ⢩ᙗ

ᒣ൷ᮠ (S.E.) ৲➗૱⿽ᮠ٬㤳ത 1 ⢩ᖱ⢩ᙗ ᴰབྷ٬ MON 87427 ሩ➗ ᴰሿ٬ 㣡㊹⍫࣋ (%) 99.7* (0.0) 98.9 (0.3) 99.2 99.6 㣡㊹ⴤᖴ} 89.9 (1.2) 90.8 (1.6) 87.2 91.5 ⌘˖䈅傼Ѫᆼޘ䲿ᵪ४㓴䇮䇑ˈ3 ⅑䟽༽˗S.E. = ḷ߶ᐞ;n=12Ǆ * 㺘᰾൘ MON 87427 ઼ሩ➗䰤ᆈ൘㔏䇑ᆖᱮ㪇ᙗᐞᔲ˄`˅Ǆ ᇊⲴǄ⺞➗૱⿽ᮠ٬㤳തᱟ׍ᦞ 4 њᐢ㓿୶ъॆ⿽ἽⲴ৲➗૱⿽Ⲵᴰሿ٬઼ᴰབྷ٬৲ 1

72 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.2.3 Շⵖᵕ ⿽ᆀⲴ㨼ਁ઼Ձⵐᵪࡦ൘н਼⢙⿽ѻ䰤ᆈ൘䖳བྷਈॆˈަ䚇Րส⹰ҏ䶎ᑨ༽ ᵲǄն⿽ᆀՁⵐ⢩ᙗ˄ྲ⺜ᇎ⿽ᆀ˅৸ᱟᵲ㥹Ⲵ䟽㾱⢩ᖱ˄Anderson, 1996; Lingenfelter and Hartwig, 2003˅Ǆ䟷⭘ḷ߶ᯩ⌅ᶕ䇴ԧἽ⢙⿽ᆀⲴ㨼ਁ઼Ձⵐ⢩ ᙗ䶎ᑨ䟽㾱Ǆᇈᯩ⿽ᆀ࠶᷀ᇦॿՊ˄AOSA˅ᱟഭ䱵кᲞ䙽䇔ਟⲴ⿽ᆀ࠶᷀⍻䈅 ᵪᶴǄAOSA ᧘㦀 20/30°C ᱟ࠶᷀⦹㊣⿽ᆀ㨼ਁⲴ⨶ᜣ⑙ᓖ˄AOSA, 2007˅Ǆ ᵜ⹄ウ䲔Ҷ䟷⭘ AOSA ᧘㦀Ⲵ⑙ᓖ˄20/30°C˅ཆˈ䘈䟷⭘ 5°Cǃ10°Cǃ20°C ઼ 30°C ᚂ⑙ԕ৺ 10/20°C ઼ 10/30°C ਈ⑙༴⨶ᶕ⍻ᇊ MON 87427ǃሩ➗઼ 7 њ ৲➗૱⿽Ⲵ⿽ᆀ㨼ਁ⦷Ǆ㻛⍻䈅Ⲵ⿽ᆀᱟ 2008 ᒤ൘㖾ഭ⡡㦧ॾᐎ઼Ժ࡙䈪Ժᐎ Ⲵ⭠䰤䈅傼᭦㧧ⲴǄᇎ傼㔃᷌≷ᙫҾ㺘 15Ǆ ṩᦞ 2 њ䈅傼ൠ⛩Ⲵᒣ൷ᮠˈMON 87427 ઼ሩ➗൘ AOSA ᧘㦀⑙ᓖ˄20/30°C˅ ԕ৺ 10°Cǃ30°Cǃ10/20°C ઼ 10/30°C лⲴ⍻䈅㔃᷌⋑ᴹԫօ㔏䇑ᆖᱮ㪇ᐞᔲ˄㺘 15˅Ǆ↔ཆˈMON 87427 ઼ሩ➗䰤൘ᡰᴹ⑙ᓖᶑԦлⲴ⺜ᇎ⿽ᆀⲮ࠶ᮠᯩ䶒䜭н ᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲǄ⿽ᆀ㨼ਁ⍻䈅㔃᷌㺘᰾ˈ൘ԕл⑙ᓖᶑԦ઼㨼ਁ⢩ᖱ⢩ᙗ ᯩ䶒лˈMON 87427 ઼ሩ➗ᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲ˖൘ 5°C ᶑԦлⲴ↫ӑ⿽ᆀᮠ ˄6.5%ሩ 11.1%˅઼ᴹ⍫࣋੨⏘⿽ᆀᮠ˄93.5%ሩ 88.9%˅˗൘ 20°C ᶑԦлⲴ㨼 ਁ⿽ᆀᮠ˄98.9%ሩ 97.1%˅઼↫ӑ⿽ᆀᮠ˄1.1%ሩ 2.9%˅Ǆ䘉Ӌᆈ൘㔏䇑ᆖᱮ 㪇ᐞᔲⲴᮠ٬ᐞ䐍䜭ᖸሿˈᒦф൘н਼⑙ᓖᶑԦл㔃᷌ᒦна㠤Ǆ↔ཆˈ൘ 5°C ᶑԦл MON 87427 Ⲵ↫ӑ⿽ᆀ઼ᴹ⍫࣋੨⏘⿽ᆀⲮ࠶ᮠԕ৺൘ 20°C ᶑԦлⲴ 㨼ਁ⿽ᆀ઼↫ӑ⿽ᆀⲮ࠶ᮠ൷൘୶ъॆ৲➗૱⿽Ⲵᮠ٬㤳ത޵Ǆഐ↔ˈ䘉Ӌ㔏䇑 ᆖᱮ㪇ᐞᔲнާᴹ⭏⢙ᆖ᜿ѹˈн㺘᰾оሩ➗⴨∄ MON 87427 ൘⿽ᆀՁⵐᡆ㨼 ਁᯩ䶒ਁ⭏Ҷ᭩ਈǄ 3.2.4 䘸ᓊᙝ 㺘 12 ޣҾߌ㢪ᙗ⣦઼㺘⧠රᙗ⣦Ⲵ⹄ウ㔃᷌㺘᰾ˈMON 87427 ઼ሩ➗൘䘲 Կǃقᓄᙗᯩ䶒⋑ᴹᐞᔲǄMON 87427 ઼ሩ➗൘ἽṚᮠǃᔰ㣡ᮓ㊹ᰦᵏǃṚ儈ǃ ӗ䟿ㅹᙗ⣦ᯩ䶒⋑ᴹᱮ㪇ᐞᔲǄMON 87427 ઼ሩ➗䰤ୟаᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲ Ⲵᙗ⣦ᱟᒬ㤇⭏⍫࣋ǄMON 87427 Ⲵᒬ㤇⭏⍫࣋㓗࡛∄ሩ➗վ˄2.7 㓗ሩ 2.4 㓗ˈ ޡ࠶ 1-9 㓗˅Ǆቭ㇑Ҽ㘵䰤ᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲˈնᒣ൷ᮠⲴᐞᔲᖸሿˈӵѪ 0.3Ǆ ᒦф MON 87427 Ⲵᒬ㤇⭏⍫࣋㓗࡛൘а਼⿽ἽⲴ୶ъॆ৲➗૱⿽Ⲵ㠚❦ਈᔲ㤳 ത޵˄1-5 㓗˅Ǆഐ↔ˈ䈕㔏䇑ᆖкⲴᱮ㪇ᐞᔲнާᴹ⭏⢙ᆖ᜿ѹǄ ਇߌъ䜘Ⲵငᢈˈߌъ䜘䖜สഐἽ⢙⧟ຳᆹޘⴁⶓỰ傼⍻䈅ѝᗳ˄䮯᱕˅᳘ ਹ᷇ⴱߌъ、ᆖ䲒Ҿ 2014 ᒤᆼᡀҶሩ MON 87427 ᣇ䲔㥹ࡲ⦹㊣Ⲵ⧟ຳᆹޘᙗ䇴 ՠǄ㔃᷌㺘᰾ˈ䖜สഐᣇ䲔㥹ࡲ⦹㊣ MON87427 ሩ㠣ቁ 2 ؽ᧘㦀ࡲ䟿௧ᯭⲴߌ 䗮䲔㥹ࡲާᴹ㢟ྭⲴ㙀ਇᙗǄ൘㦂ൠ઼ṭษൠ䈅傼ᶑԦлˈ䖜สഐ⦹㊣ MON87427 Ⲵ⭏ᆈㄎҹ㜭࣋оަሩᓄⲴ䶎䖜สഐ⦹㊣൷ᰐᱮ㪇ᐞᔲǄ ⭡↔ਟԕᗇࠪ㔃䇪ˈMON 87427 ઼ሩ➗൘䘲ᓄᙗᯩ䶒⋑ᴹᱮ㪇ᙗᐞᔲǄ

73 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.2.5 ⭕ᆎㄔҿ㜳࣑ MON 87427 ઼ሩ➗Ⲵߌ㢪ᙗ⣦઼㺘⧠රᙗ⣦⹄ウवᤜҶἽṚ⭏䮯ǃ㑱⇆ǃ Կ઼㩭㋂˅ㅹⲴ䇴ՠˈ㔃᷌˄㺘 12˅㺘᰾ MON 87427 ઼ሩقྲ˄ӗ䟿ǃ㩭㋂ᙗ ➗ѻ䰤൘䘉Ӌᙗ⣦ᯩ䶒⋑ᴹᐞ࡛ǄਖཆˈՐ᫝㜭઼࣋ᯩᔿ˄㺘 13˅઼⿽ᆀ㨼ਁ Ձⵐ⢩ᙗ˄㺘 15˅Ⲵ⹄ウ㔃᷌ҏ㺘᰾ MON 87427 ઼ሩ➗ѻ䰤⋑ᴹᐞᔲǄ о⧟ຳѝަᆳ⭏⢙Ⲵ⴨ӂ֌⭘ޣ㌫ԕ৺⧟ຳ㛱䘛ᯩ䶒Ⲵ䇴ՠҏਟѪ䇴ՠ MON 87427 䖜ਈѪᴹᇣ⭏⢙Ⲵਟ㜭ᙗԕ৺⭏ᆈㄎҹ㜭࣋Ⲵᨀ׋׍ᦞǄ ޣҾ MON 87427 ሩ䶎⭏⢙㛱䘛Ⲵ৽ᓄˈ䇴ԧⲴ޵ᇩवᤜ˖վ⑙ǃᰡǃ⏍ǃ 䵌߫ǃߠ䴩ǃ儈⑙ǃⸯ⢙∂㍐ǃ≞㕪ཡǃ㩕ޫ㕪ཡǃ൏༔ᶯ㔃ǃ൏༔≤価઼৺仾 ㅹǄ൘ 172 亩⅑Ⲵᮠᦞሩ∄ѝˈMON 87427 ઼ሩ➗ѻ䰤Ⲵ㿲ሏ㓗࡛൷ᰐᐞᔲ˄㺘 ˅ǄޣҾ MON 87427 ሩ⯵ᇣ㛱䘛Ⲵ৽ᓄˈ䇴ԧⲴ޵ᇩवᤜ⛝⯭⯵ǃ㓶㧼ᙗਦ ᷟ⯵ǃ㓶㧼ᙗਦᯁ⯵ǃ唁✏➔⯵ǃ㽀ᯁ⯵ǃょ㞀⯵ǃ⵬ᯁ⯵ǃ䮠ᆒ㧼ǃ⚠ᯁ⯵ǃ ㊭㋂㓒㢢ᶑ㓩⯵ǃਦᷟ⯵ǃ⦹㊣⸞㣡ਦ⯵∂ǃབྷᯁ⯵ǃ㍛㤇㔬ਸ⯷ǃ㞀䴹⯵ǃ㓩 ᷟ⯵ǃṩ㞀⯵ǃ䬸⯵ǃ㤇ᷟ⯵ǃ㤇㞀⯵ǃ唁ょ⯵ǃሿᯁ⯵ǃ㤾㞀⯵઼⦹㊣㓶㧼ᙗ ᷟ㨾⯵ㅹ⯵ᇣǄ൘ᙫޡ 210 亩⅑Ⲵ∄䖳ѝˈMON 87427 ઼ሩ➗ѻ䰤Ⲵ㿲ሏ㓗࡛ ൷ᰐᐞᔲ˄㺘 16˅Ǆ 2014 ᒤߌъ䜘䖜สഐἽ⢙⧟ຳᆹޘⴁⶓỰ傼⍻䈅ѝᗳ˄䮯᱕˅ሩ MON 87427 Ⲵ⭏ᆈㄎҹ㜭࣋䘋㹼Ự⍻㔃᷌㺘᰾˖˄1˅൘㦂ൠ䈅傼ᶑԦлˈ䖜สഐ⦹㊣ MON 87427 Ⲵ⭏ᆈㄎҹ㜭࣋ᱮ㪇ᕡҾᵲ㥹ˈфоަሩᓄⲴ䶎䖜สഐ⦹㊣ᰐᱮ㪇 ᐞᔲ˗˄2˅൘ṭษൠ䈅傼ᶑԦлˈ䖜สഐ⦹㊣ MON87427 ൘Ṛ儈ǃ㾶ⴆᓖǃӗ 䟿ǃਾԓ⿽ᆀਁ㣭⦷ㅹᯩ䶒ˈоሩᓄⲴ䶎䖜สഐ⦹㊣ᰐᱮ㪇ᐞᔲǄ ٮ⢙㔬ਸк䘠㔃᷌ˈMON 87427 ઼ሩ➗൘⭏ᆈㄎҹ㜭࣋ԕ৺䖜ਈѪᴹᇣ⭏ ੁᯩ䶒⋑ᴹᐞ࡛Ǆ

74 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘14. 2008ᒤ㖾ഭ⭠䰤䈅傼ѝMON 87427઼ᑨ㿴ሩ➗ሩ䶎⭏⢙㛱䘛৽ᓄ㓗࡛Ⲵ ሩ∄㔃᷌≷ᙫ

䶎⭏⢙㛱䘛 ᡰᴹ䈅傼⛩㿲ሏሩ∄亩 MON 87427оሩ➗䰤ᰐᐞᔲⲴ ⅑ᮠ1 㿲ሏሩ∄亩⅑ᮠ ਸ䇑 172 172

վ⑙ 66

ᒢᰡ 13 13

⍚⏍ 19 19

䵌߫ 44

ߠ䴩 26 26

儈⑙ 25 25

ⸯ⢙∂㍐ 11

≞㕪ཡ 22

㩕ޫ㕪ཡ 13 13

൏༔ᶯ㔃 77

≤価઼൏༔2 77

仾 49 49

⌘˖䈅傼䟷⭘ᆼޘ䲿ᵪ४㓴䇮䇑ˈ3⅑䟽༽Ǆ䈳ḕᮠᦞ䰤ᱟ੖ᆈ൘ᐞᔲᱟสҾ㿲ሏ㓗࡛ ᶕࡔᯝˈ㘼䶎ṩᦞ㔏䇑࠶᷀Ǆ൘⦹㊣⭏䮯Ⲵ4њ䱦⇥䘋㹼䈳ḕ˖V2~V4ǃV10~V15ǃ VT~R3ǃR6Ǆ 1 䈅傼ൠ⛩वᤜ˖IA1 = Wapello Co., IA; IA2 = Benton Co., IA; IL1 = Stark Co., IL; IL2 = Clinton Co., IL; IN1 = Montgomery Co., IN; IN2 = Boone Co., IN; MI = Ottawa Co., MI; MO = Butler Co., MO; OK = Caddo Co., OK; PA = Berks Co., PA; TX = Armstrong Co., TX; WI = Walworth Co., WI; ARNE = Jackson Co., AR; IARL = Jefferson Co., IA; ILWY = Stark Co., IL; INRC = Parke Co., IN.

2 वᤜ╛♼઼≤価઼൏༔

75 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘15. MON 87427઼ሩ➗⿽ᆀⲴ㨼ਁ⢩ᖱ⢩ᙗ⍻ᇊ㔃᷌

⑙ᓖ༴⨶ ᒣ൷ᮠ %(S.E.)1 ৲➗૱⿽ᮠ٬㤳ത 2 㨼ਁ⢩ᖱ⢩ᙗ (°C) MON 87427 ሩ➗ Min Max 5 㨼ਁ⿽ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ᴹ⍫࣋⺜ᇎ⿽ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ↫ӑ⿽ᆀ 6.5* (1.5) 11.1 (2.5) 3.8 6.8 ᴹ⍫࣋੨⏘⿽ᆀ 93.5* (1.5) 88.9 (2.5) 93.3 96.3 10 㨼ਁ⿽ᆀ 82.4 (2.4) 79.0 (1.8) 87.3 98.3 ᴹ⍫࣋⺜ᇎ⿽ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ↫ӑ⿽ᆀ 3.9 (0.8) 4.4 (0.6) 1.0 5.3 ᴹ⍫࣋੨⏘⿽ᆀ 13.8 (1.9) 16.6 (1.8) 0.0 11.3 20 㨼ਁ⿽ᆀ 98.9* (0.3) 97.1 (0.6) 95.8 99.3 ᴹ⍫࣋⺜ᇎ⿽ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ↫ӑ⿽ᆀ 1.1* (0.3) 2.9 (0.6) 0.8 4.3 ᴹ⍫࣋੨⏘⿽ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 30 㨼ਁ⿽ᆀ 97.4 (0.5) 98.4 (0.5) 97.0 99.8 ᴹ⍫࣋⺜ᇎ⿽ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ↫ӑ⿽ᆀ 2.6 (0.5) 1.6 (0.5) 0.3 3.0 ᴹ⍫࣋੨⏘⿽ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 10/20 㨼ਁ⿽ᆀ 98.0 (0.5) 98.4 (0.6) 95.8 99.8 ᴹ⍫࣋⺜ᇎ⿽ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ↫ӑ⿽ᆀ 2.0 (0.5) 1.6 (0.6) 0.3 4.3 ᴹ⍫࣋੨⏘⿽ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 10/30 㨼ਁ⿽ᆀ 98.5 (0.3) 97.9 (0.6) 97.0 99.5 ᴹ⍫࣋⺜ᇎ⿽ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ↫ӑ⿽ᆀ 1.5 (0.3) 2.1 (0.6) 0.5 3.0 ᴹ⍫࣋੨⏘⿽ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 20/30 ↓ᑨ㨼ਁ⿽ᆀ 94.4 (0.9) 94.9 (0.9) 93.8 98.8 (AOSA ᧘㦀) 䶎↓ᑨ㨼ਁ⿽ᆀ 3.8 (1.2) 3.6 (1.1) 0.8 3.8 ᴹ⍫࣋⺜ᇎ⿽ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0 ↫ӑ⿽ᆀ 1.9 (0.5) 1.5 (0.3) 0.3 4.0 ᴹ⍫࣋੨⏘⿽ᆀ† 0.0 (0.0) 0.0 (0.0) 0.0 0.0

⌘˖2 њ䈅傼ൠ⛩ˈ䈅傼䇮䇑Ѫ㻲४䇮䇑ˈ4 ⅑䟽༽ˈn=8Ǆ * 㺘᰾൘ MON 87427 ઼ሩ➗䰤ᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲ˄`˅Ǆ † ⭡Ҿ㕪ቁᮠᦞ㘼ᰐ⌅䘋㹼㔏䇑ᆖ∄䖳Ǆ 1 ൘ḀӋᛵߥлˈ⭡Ҿ㔏䇑ᒣ൷ᮠᰦ䟷⭘ 4 㠽 5 ޕˈ਴㊫⿽ᆀⲴⲮ࠶ᮠѻ઼ਟ㜭нㅹ Ҿ 100%ˈS.E. = ḷ߶ᐞǄ ᇊⲴǄ⺞➗૱⿽ᮠ٬㤳തᱟ׍ᦞ 4 њᐢ㓿୶ъॆ⿽ἽⲴ৲➗૱⿽Ⲵᴰሿ٬઼ᴰབྷ٬৲ 2

76 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘16. 2008ᒤ㖾ഭ⭠䰤䈅傼ѝMON 87427઼ᑨ㿴ሩ➗ሩ⯵ᇣ৽ᓄ㓗࡛Ⲵሩ∄㔃᷌

⯵ᇣ㛱䘛 ᡰᴹ䈅傼⛩㿲ሏሩ∄ MON 87427оሩ➗䰤ᰐᐞᔲ 亩⅑ᮠ1 Ⲵ㿲ሏሩ∄亩⅑ᮠ ਸ䇑 210 210 ⛝⯭⯵ 12 12 㓶㧼ᙗਦᷟ⯵ 11 㓶㧼ᙗਦᯁ⯵ 11 唁✏➔⯵ 11 㽀ᯁ⯵ 11 ょ㞀⯵2 17 17 ⵬ᯁ⯵ 88 䮠㜎㧼˄Fusarium˅ 11 11 ⚠ᯁ⯵3 32 32 ㊭㋂㓒㢢ᶑ㓩⯵ 22 ਦᷟ⯵ 55 ⦹㊣⸞㣡ਦ⯵∂⯵ 11 བྷᯁ⯵ 17 17 ㍛㤇㔬ਸ⯷ 11 㞀䴹⯵˄Pythium4˅ 99 㓩ᷟ⯵˄Rhizoctonia˅ 22 ṩ㞀⯵ 66 䬸⯵5 33 33 㤇ᷟ⯵ 12 12 㤇㞀⯵ 22 唁ょ⯵ 77 ሿᯁ⯵ 88 㤾㞀⯵2 16 16 ⦹㊣㓶㧼ᙗᷟ㨾⯵ 55 ⌘˖䈅傼䟷⭘ᆼޘ䲿ᵪ४㓴䇮䇑ˈ3⅑䟽༽Ǆ䈳ḕᮠᦞ䰤ᱟ੖ᆈ൘ᐞᔲᱟสҾ㿲ሏ㓗࡛ ᶕࡔᯝˈ㘼䶎ṩᦞ㔏䇑࠶᷀Ǆ൘⦹㊣⭏䮯Ⲵ4њ䱦⇥䘋㹼䈳ḕ˖V2~V4ǃV10~V15ǃ VT~R3ǃR6Ǆ 1 䈅傼ൠ⛩वᤜ˖IA1 = Wapello Co., IA; IA2 = Benton Co., IA; IL1 = Stark Co., IL; IL2 = Clinton Co., IL; IN1 = Montgomery Co., IN; IN2 = Boone Co., IN; MI = Ottawa Co., MI; MO = Butler Co., MO; OK = Caddo Co., OK; PA = Berks Co., PA; TX = Armstrong Co., TX; WI = Walworth Co., WI; ARNE = Jackson Co., AR; IARL = Jefferson Co., IA; ILWY = Stark Co., IL; INRC = Parke Co., IN. 2 ᭦㧧ᰦ䇴ՠⲴょ㞀⯵઼㤾㞀⯵वᤜ5Ṛ䶎㌫㔏䘹ᤙⲴ⠶㻲㤾⿶Ǆㅜй⅑䈳ḕᰦ ˄VT~R3˅໎࣐Ҷа⅑ょ㞀⯵䈳ḕǄ 3 वᤜ⚠ਦ䴹⯵˄gray leaf mold˅Ǆ 4 䬸⯵वᤜ㽀䬸⯵઼ਦ䬸⯵Ǆ

77 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.2.6 䖢ะഖὃ⢟Ⲻ䚍Ֆ⢟䍞ੇެᆹὃ⢟Ƚࣞ⢟ૂᗤ⭕⢟ਇ⭕䖢〱Ⲻ ਥ㜳ᙝ аᰖᮤਸ䘋Ἵ⢙สഐ㓴ˈT-DNA Ⲵ䗩⭼ᒿࡇቡՊђཡǄ⭡Ҿ㕪ቁ䗩⭼ᒿࡇ ⲴᑞࣙˈᮤਸⲴ DNA 䖜〫ࡠަԆἽ⢙䟼Ⲵਟ㜭ᙗࠐѾ⋑ᴹˈ䲔䶎䙊䗷ᴹᙗᵲӔ ˄Huttner et al., 1992; Bakkeren et al., 1989˅Ǆ 㣡㊹ӻሬⲴสഐ┲〫˄䙊ᑨ〠ѪᔲӔᦸ㊹ᡆสഐ⑇ޕ˅ᱟањᡆཊњสഐᮤ ਸࡠਇփสഐ㓴Ⲵ䟽㾱䙄ᖴǄสഐ⑇ޕ䙊ᑨਇ⭏⢙઼䶎⭏⢙ഐ㍐Ⲵᖡ૽ˈ∄ྲἽ ⭏⢙ᆖ⢩ᖱǃ㣡㊹⭏⢙ᆖ⢩ᖱ઼㣡㊹䟿ǃ׋փ઼ਇփⲴ㣡ᵏ䟽ਐᰦ䰤ǃ㣡㊹׋⢙ փ઼ਇփⲴӢ઼〻ᓖԕ৺⑙ᓖǃ⒯ᓖ઼ߌ㢪㙅֌ㅹઘ䗩⧟ຳǄสഐ⑇ޕᱟа⿽㠚 ❦⧠䊑ˈഐ↔⋑ᴹᗵ㾱䗷ཊޣ⌘สഐ⑇ޕ䗷〻ˈ㘼ᓄ䟽⛩䇴ԧᨂޕⲴཆⓀสഐᱟ ੖ᆹޘԕ৺ਁ⭏สഐ⑇ޕᱟ੖Պ໎࣐䖜ਈѪᴹᇣ⭏⢙Ⲵ▌࣋Ǆ ᔲӔᦸ㊹ᱟ⦹㊣Ⲵപᴹ⢩ᙗˈഐ↔൘ṭษ⦹㊣⿽˄Zea mays L.˅޵ਁ⭏㣡㊹ ӻሬⲴสഐ┲〫ᱟᗵ❦ⲴǄབྷ䟿Ⲵ⹄ウਁ⧠˖1˅⭡ҾᔲӔᦸ㊹ˈṭษ⦹㊣⿽޵ Ⲵสഐ┲〫仁⦷ᱟ⴨ᖃ儈Ⲵ˗2˅ਁ⭏สഐ┲〫ⲴⲮ࠶ᮠ䲿⦹㊣㗔փǃᵲӔ⿽ᡆ 㠚Ӕ㌫Ⲵн਼㘼н਼˗3˅㣡㊹Ⓚ઼ਇփⲴ䐍⿫䎺䘌ˈ┲〫Ⲵ≤ᒣቡ䎺վ˗4˅⧟ ຳഐ㍐ਟԕᖡ૽สഐ┲〫Ⲵ〻ᓖ˗5˅൘⭠䰤ᶑԦл⦹㊣㣡㊹㋂Ⲵ⭏⍫࣋ᱟᖸ⸝ Ⲵ˗6˅⦹㊣ᮓ㊹Ⲵᰦᵏ⴨ሩ䖳䮯˗7˅⦹㊣ਟԕ䙊䗷仾Ⴢᡆ㲛ჂՐ㊹Ǆ аӋ⹄ウ㺘᰾ˈṭษ⦹㊣⿽޵Ⲵสഐ┲〫〻ᓖՊṩᦞ䈅傼䇮䇑ǃ⧟ຳᶑԦ઼ Ự⍻ᯩ⌅Ⲵн਼㘼ਈॆǄᙫⲴᶕ䈤ˈྲ᷌㣡㊹ⓀоਇփⲴ䐍⿫䎵䗷 200 mˈਁ⭏ สഐ┲〫Ⲵਟ㜭ᙗቡՊ䱽ࡠ 1%ԕлǄ䘉ᯩ䶒ؑ᚟ሩ൘⦹㊣㛢⿽ǃ⿽ᆀ⭏ӗǃ⿽ ᆈ৺ަᆳᓄ⭘ᰦᴹ᭸ൠ᧗ࡦสഐ┲〫ᱟ䶎ᑨᴹ⭘ⲴǄ؍ᙗ 㠣Ҿ MON 87427 ੁަᆳ⦹㊣ਁ⭏สഐ┲〫нՊᕅ䎧ᆹޘᯩ䶒Ⲵ亮㲁ˈഐѪ ޘᓧ઼⧟ຳᆹڕབྷ䟿⹄ウᐢ㓿䇱᰾ MON 87427 वਜ਼Ⲵ cp4 epsps สഐнՊሩӪ㊫ ᑖᶕডᇣǄ ⦹㊣઼аᒤ⭏໘㾯କ㊫㴰哽˄Zea mays subsp. mexicana˅໘㾯କ઼ডൠ傜᣹ ⲴаӋൠ४ྲ᷌⿽Ἵ䐍⿫ᖸ䘁ˈਟਁ⭏㠚❦⴨ӂᦸ㊹ᒦ㺘⧠ᵲӔӢ઼ᙗ˄Wilkes, Ǆն Ellstrand ㅹ˄2007˅Ⲵ⹄ウ㺘᰾ˈণ֯ሶ⦹㊣઼໘㾯କ⦹㴰哽⿽൘а˅1972 䎧ˈҼ㘵ѻ䰤ਁ⭏ᔲӔⲴ仁⦷䘈ᱟᖸվⲴǄྲ᷌㘳㲁ᔰ㣡ᰦᵏǃൠ⨶䐍⿫䳄⿫઼ ਁ㛢〻ᓖㅹഐ㍐ˈҼ㘵нཚਟ㜭ਁ⭏㠚❦ᔲӔǄ ণ֯䟷⭘⢩↺ᢰᵟˈ⦹㊣о᪙ᬖ⿮኎Ⲵཊᒤ⭏⢙⿽ⲴᵲӔҏᱟ䶎ᑨഠ䳮ⲴǄ ,ཆˈণ֯ᦸ㊹ᵲӔᡀ࣏ˈަਾԓҏՊн㛢ᒦф൘䚇ՐкҏᱟнっᇊⲴ˄Galinat↔ 1988; Russell and Hallauer, 1980˅Ǆ 㔬кᡰ䘠ˈMON 87427 ѝⴞⲴสഐ䙊䗷ᴹᙗᵲӔ䖜〫Ⲵ㤳തਚ䲀Ҿਟԕ઼ ṭษ⦹㊣ᵲӔӢ઼Ⲵ⿽኎ˈа㡜Ѫṭษ⦹㊣⿽޵ⲴаӋ૱⿽ǄੁަᆳἽ⢙⢙⿽┲ 〫Ⲵਟ㜭ᙗࠐѾнᆈ൘Ǆ ྲ਼൘ 1.4.4 䜘࠶᧿䘠Ⲵ䛓ṧˈⴞࡽц⭼к䘈⋑ᴹⴤ᧕䇱ᦞ㺘᰾ᐢ㓿ਁ⭏䗷 Ἵ⢙䚇Րสഐੁࣘ⢙઼ᗞ⭏⢙ѻ䰤Ⲵ≤ᒣ䖜〫˄horizontal transfer˅˄Bertolla and Simonet, 1999˅Ǆ

78 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.2.7 䖢਎ᡆᵸ㥿Ⲻਥ㜳ᙝ ⦹㊣ᱟ儈ᓖ傟ॆ֌⢙ˈ⋑ᴹӪ䇔Ѫ⦹㊣ቡᱟᵲ㥹ᡆ㘵Պ䖜ਈѪᵲ㥹˄Crockett, 1977; Muenscher, 1980˅ˈ㖾ഭ᭯ᓌҏ⋑ᴹሶ⦹㊣ࡇѪᴹᇣᵲ㥹ⴞᖅѝ˄㿱⌅㿴 7CFR§360˅Ǆ↔ཆˈ⦹㊣ᐢ㓿൘ޘ⨳ᒯ⌋⿽Ἵˈ㠣Ӻ⋑ᴹԫօᣕ੺䇔Ѫ⦹㊣኎ Ҿᵲ㥹Ǆ㓿䗷䮯ᰦ䰤Ⲵ䘹ᤙ઼傟ॆˈ⦹㊣нާ༷ᵲ㥹Ⲵสᵜ⢩ᖱ˄ྲ⿽ᆀՁⵐ⢩ ᙗ઼ᢙᮓᵪࡦ˅ˈ⿫ᔰӪᐕṭษ⧟ຳሶн㜭⭏ᆈ˄Galinat, 1988; Keeler, 1989˅Ǆ⦹ ㊣᷌ょ⭡㤎ਦव㼩ˈঅњ㊭㋂ᖸ䳮൘㠚❦Ր᫝Ǆণ֯⦹㊣㊭㋂൘䘀䗃䗷〻ѝਟ㜭 Պਁ⭏䚇᫂ˈն⦹㊣ᖸ䳮൘⋏䗩઼䐟䗩ᖒᡀ㠚⭏㤇ᒦ⭏䮯ᡀ⟏Ǆণ֯⦹㊣൘⭠䰤 ਟԕᖒᡀ㠚⭏㤇ˈᖸ䳮䘳㝡䎺ߜǃ֌⢙䖞֌ǃᵪỠॆ㙅⿽ԕ৺ॆᆖ㦟ࡲ༴⨶ㅹഐ ㍐Ⲵᖡ૽ˈ䘋㘼൘л㥜⭏䮯ᡀ⟏Ǆ ԕк 3.2.1~3.2.5 䜘࠶Ⲵ࠶᷀㺘᰾ˈMON 87427 ઼ᑨ㿴ሩ➗൘⭏⇆㜭࣋ǃ⿽ ᆀՁⵐ⢩ᙗǃ䘲ᓄᙗ઼⭏ᆈㄎҹ㜭࣋ㅹᯩ䶒⋑ᴹᱮ㪇ᙗᐞᔲ˗оᑨ㿴ሩ➗⴨∄ˈ MON 87427 нՊ໎࣐ަ䖜ਈѪᵲ㥹Ⲵਟ㜭ᙗǄ 3.2.8 ᣍ⯻㲡䖢ะഖὃ⢟ሯ䶬ḽ⭕⢟਀䶔䶬ḽ⭕⢟Ⲻᖧଃθऻᤢሯ⧥ ູѣᴿⴀૂᴿᇩ⭕⢟Ⲻᖧଃ MON 87427 㺘䗮Ⲵ CP4 EPSPS 㳻ⲭ˄5-✟䞷щᔿ䞞䞠㧭㥹䞨-3-⼧䞨ਸ䞦˅ ᱟἽ⢙઼ᗞ⭏⢙փ޵㣣俉᯿≘ส䞨ԓ䉒䙄ᖴѝⲴа⿽⼧䞨ส䖜〫䞦ˈн኎Ҿᣇ⯵ ᡆᣇ㲛สഐˈഐ↔нᆈ൘䶦ḷᡆ䶎䶦ḷ⭏⢙Ǆ ൘ 2008 ᒤ㖾ഭⲴ⭠䰤䈅傼ѝˈ䈳ḕҶ㢲㛒ࣘ⢙ⲴѠᓖˈ䈳ḕⲴ㢲㛒ࣘ⢙व ᤜ˖㳌㲛ǃ⦹㊣ཌ㴮ǃ⦹㊣䐣⭢ǃ㵑㵹ǃ㶇㲛ǃਦ㵹ǃेᯩ⦹㊣伏ṩ㲛ǃ䵢ቮ⭢ǃ ইᯩ⦹㊣伏ṩ㲛ǃ㾯ᯩ⦹㊣伏ṩ㲛ǃ㵈㴋ǃབྷ⵬⭢㲛ǃ㽀㥹㳹ǃ㔯㥹㳹ǃ⬒㲛ǃ བྷරᇴ⭏ᙗ㟌㗵ⴞᰶ㲛ǃሿරᇴ⭏ᙗ㟌㗵ⴞᰶ㲛ǃလ㶭઼ሿ㣡㶭Ǆޡᴹ 4 њ䈅傼 ൠ⛩ˈ⇿њൠ⛩࠶ 4 њᰦᵏ䈳ḕǄ൘ᙫޡ 203 亩⅑䈳ḕⲴᮠᦞሩ∄ѝˈᴹ 193 亩⅑൘ MON 87427 ઼ሩ➗䰤нᆈ൘㔏䇑ᆖᱮ㪇ᙗᐞᔲˈᴹ 12 亩⅑ᆈ൘㔏䇑ᆖᱮ 㪇ᙗᐞᔲ˄㺘 ˅Ǆቭ㇑൘ḀӋ䈅傼⛩ⲴḀӋ㢲㛒ࣘ⢙ⲴḀ⅑䈳ḕѝ MON 87427 ઼ሩ➗䰤ᆈ൘㔏䇑ᆖᱮ㪇ᙗᐞᔲˈն䘉Ӌ㔏䇑ᆖᐞᔲ൘਼а⿽㢲㛒ࣘ⢙Ⲵн਼ൠ ⛩઼н਼䈳ḕᰦᵏⲴ㺘⧠ᒦна㠤Ǆሶ MON 87427 Ⲵᮠ٬о୶ъॆ৲➗૱⿽Ⲵ ᮠ٬⴨∄ˈ㾱Ѹ MON 87427 Ⲵᮠ٬൘৲➗૱⿽Ⲵᮠ٬㤳ത޵ˈ㾱Ѹ MON 87427 Ⲵᮠ٬о৲➗૱⿽Ⲵᮠ٬㤳തᐞ䐍ᖸሿǄഐ↔ˈਟԕᗇࠪ㔃䇪䇔Ѫ㿲ሏࡠⲴ㔏䇑 ᆖᱮ㪇ᐞᔲнާᴹ⭏⢙ᆖ᜿ѹˈҏоሬޕⲴཆⓀสഐᰐޣǄ 2014 ᒤˈߌъ䜘䖜สഐ⧟ຳᆹޘⴁⶓỰ傼⍻䈅ѝᗳ˄䮯᱕˅Ự⍻Ҷ MON 87427 ሩ⭏⢙ཊṧᙗⲴᖡ૽ˈ㔃᷌㺘᰾ˈо䶎䖜สഐ⦹㊣⴨∄ˈ䖜สഐ⦹ ㊣ MON87427 ሩ⭠䰤ѫ㾱㢲㛒ࣘ⢙ᮠ䟿઼㗔㩭㔃ᶴǃѫ㾱匎㗵ⴞᇣ㲛ԕ৺ѫ㾱 ⯵ᇣᰐᱮ㪇ᖡ૽Ǆ

79 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘17. 2008ᒤ㖾ഭ⭠䰤䈅傼ѝMON 87427઼ᑨ㿴ሩ➗к㢲㛒ࣘ⢙Ѡᓖ㺘⧠ࠪᱮ㪇 ᙗᐞᔲⲴ㔃᷌≷ᙫ

ḷ߶ᐞ˅ ৲➗૱⿽ᮠ٬˄䈅傼 ᒣ൷٬ 㢲㛒ࣘ⢙ 䈳ḕᰦᵏ ൠ⛩ MON 87427 ሩ➗ 㤳ത

ᴹᇣ㢲㛒ࣘ⢙ ⦹㊣䐣⭢ PA V10~V15 91.7 (26.3)* 59.0 (13.7) 30.3 - 120.3 ⦹㊣䐣⭢ PA R3~R4 12.0 (2.5)* 5.3 (1.5) 3.3 - 10.3 㶇㲛 MO R2 1.3 (0.9)* 0.0 (0.0) 0.0 - 1.0 ਦ㵹 PA V18~VT 7.7 (2.9)* 2.0 (1.2) 2.3 - 10.7

ᴹ⳺㢲㛒ࣘ⢙ 㵈㴋 PA R2 2.3 (0.3)* 0.7 (0.7) 1.0 - 3.7 ⬒㲛 IL1 R1 0.3 (0.3)* 1.3 (0.3) 0.0- 0.3 ⬒㲛 IN2 R1 10.7 (2.8)* 3.0 (1.0) 2.0 - 7.0 བྷරᇴ⭏ᙗ㟌㗵ⴞ IN2 R2 26.7 (3.2)* 9.7 (2.2) 9.3 - 14.7 བྷරᇴ⭏ᙗ㟌㗵ⴞ IN2 R3~R4 47.7 (5.5)* 27.7 (5.3) 29.7 - 36.3 လ㶭 PA V10~V15 0.7 (0.3)* 2.7 (0.7) 1.3 - 4.7 ሿ㣡㶭 IN2 V18~VT 0.3 (0.3)* 3.7 (1.5) 0.3 - 3.3 ሿ㣡㶭 PA R3~R4 14.0 (1.2)* 7.3 (0.7) 8.7 - 17.3 ⌘˖䈅傼䟷⭘ᆼޘ䲿ᵪ४㓴䇮䇑ˈ3⅑䟽༽Ǆ൘⦹㊣⭏䮯Ⲵ5њ䱦⇥䘋㹼䈳ḕ˖V10~V15ǃ V18~VTǃR1ǃR2ǃR3~R4Ǆ 1 䈅傼ൠ⛩वᤜ˖IL1 = Stark Co., IL; IN2 = Boone Co., IN; MO = Butler Co., MO; PA = Berks Co., PAǄ ሯ⭕ᘷ⧥ູⲺެᆹᴿⴀᡌᴿᇩ֒⭞ 3.2.9 ࡠⴞࡽѪ→ˈ䘈⋑ᴹ䇱ᦞ㺘᰾ MON 87427 ᡆ㘵ަ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭՊ ሩ⭏ᘱ⧟ຳӗ⭏н㢟ᖡ૽ǄCP4 EPSPS ਟԕ൘ MON 87427 Ⲵ㩕ޫ⭏䮯ಘᇈ઼䳼 ᙗ⭏⇆ಘᇈ↓ᑨ㺘䗮ˈն൘䳴ᙗ⭏⇆ಘᇈ˄∑㔂ቲ઼㣡㊹ሿᆒᆀ˅н㺘䗮ᡆ㺘䗮䟿ᖸվǄഐ↔൘ᵲӔ⿽ࡦ⿽ᰦ௧ᯭ㥹⭈㟖ˈਟԕ֯ MON 87427 Ⲵ㣡㊹䍕㛢ˈ൘ Ḁ⿽〻ᓖк߿ቁަ┲〫Ⲵਟ㜭ᙗǄ

80 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᓭᖧଃᯯ䶘Ⲻᐤᔸڛ㊱䖢ะഖὃ⢟ф਍։ᡌӨᵢὃ⢟൞ሯӰ 3.3 ྲࡽᡰ䘠ˈMON 87427 ᱟ䙊䗷ߌᵶ㧼ӻሬ䖜ॆˈሶ PV-ZMAP1043 䍘㋂ѝ Ⲵ T-DNA ሬޕࡠ LH198 × HiII ਇփ૱㌫ѝ㧧ᗇⲴǄ䈕 T-DNA वਜ਼ᴹањ cp4 epsps สഐ㺘䗮ⴂˈवᤜ e35S ੟ࣘᆀǃhsp70 Ⲵ޵ਜ਼ᆀǃCTP2 ਦ㔯փ䖜〫㛭 ᒿࡇǃcp4 epsps 㕆⸱ᒿࡇ઼ nos 㓸→ᆀǄ 㳻ⲭ䍘⢩ᖱ⢩ᙗ⹄ウ㔃᷌㺘᰾ˈMON 87427 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭоᐢ 㓿൘ѝഭԕ৺ц⭼ަԆഭᇦ㧧ᗇᢩ߶Ⲵཊ⿽ᣇ㥹⭈㟖֌⢙ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭᱟ⴨਼ⲴǄ䘉Ӌӗ૱ѝⲴ CP4 EPSPS 㳻ⲭⲴᆹޘᙗᐢ㓿㻛བྷ䟿Ⲵᆹޘᙗ䇴 ԧ⹄ウᡰ䇱ᇎǄ ཆˈ֯⭘ᴰᯠ؞䇒઼ᴤᯠⲴ䗷᭿৏ǃ䞷ⓦ㳻ⲭ઼哖䉧㳻ⲭᒿࡇᮠᦞᓃ↔ ˄AD_2016˅ǃ∂㍐ᮠᦞᓃ˄TOX_2016˅ǃGenBank 㳻ⲭ䍘ᮠᦞᓃ˄PRT_2016˅ǃ GenBank 㺘䗮ᒿࡇḷㆮᮠᦞᓃ˄EST˅ǃ䶎߇։Ṩ㤧䞨ᮠᦞᓃ˄NT_2016˅ԕ৺ 䶎߇։≘ส䞨ᮠᦞᓃ˄NR_2016˅ሩ MON 87427 ᨂޕս⛩䱴䘁४ฏ䘋㹼ޘ䶒Ⲵ ٷ㔃⛩४㘫䈁㘼ᗇⲴ 14 ⿽ޕ㘬ᒿࡇ-ᨂח’⭏⢙ؑ᚟ᆖ࠶᷀Ǆवᤜ˖֯⭘Ӿ 5’ᡆ 3 ᇊᒿࡇԕ৺Ӿ T-DNA 㘫䈁Ⲵ 6 ⿽䈫⸱ṶѪỰ㍒ᒿࡇˈ൘ AD_2016ǃTOX_2016 ઼ PRT_2016 ᮠᦞᓃѝ࠶࡛䘋㹼 FASTA ઼ޛ≘ส䞨━ࣘデਓᩌ㍒Ǆᩌ㍒㔃᷌㺘 ᇊᒿࡇ઼Ӿ T-DNA 㘫䈁Ⲵ 6 ⿽䈫⸱Ṷ൘Ἵ⢙ѝᆈ൘Ⲵٷ᰾ˈ㲭❦⨶䇪к䘉 14 ⿽ ᓧⲴ∂㍐㳻ⲭ઼ڕ⢙ਟ㜭ᙗᶱվˈণ֯ᆈ൘ˈ䘉Ӌᒿࡇоᐢ⸕ਟ㜭ᖡ૽Ӫ㊫ᡆࣘ ᣇ㩕ޫഐᆀǃ㠤᭿㳻ⲭ઼࣏㜭⍫ᙗ㳻ⲭѻ䰤൷ᰐᱮ㪇ᒿࡇ⴨լᙗᡆ਼ⓀᙗǄሶ 㘬ᒿࡇ൘ EST_2016 ઼ NT_2016 ᮠᦞᓃחս⛩䱴䘁Ⲵสഐ㓴ޕMON 87427 ѝᨂ ѝ䘋㹼 BLASTn ࠶᷀ˈ൘ NR_2016 ᮠᦞᓃѝ䘋㹼 BLASTx ࠶᷀ˈ൷ᵚਁ⧠ MON ˈ㘬สഐ㓴 DNA ޵䜘ᡆ䱴䘁ᆈ൘ਟ㜭㕆⸱ᯠ㳻ⲭӗ⢙Ⲵ ORFחս⛩ޕᨂ 87427 ҏ⋑ᴹ޵ⓀᙗⲴ ORF ഐѪ T-DNA Ⲵᨂޕ㘼㻛⹤ൿǄ 3.3.1 ∈ᙝ 䇴ԧᯠᕅޕ㳻ⲭ▌൘Ⲵ∂ᙗ઼㠤᭿ᙗԕ৺㟣伏ᆹޘᙗᰦˈ䈕㳻ⲭᱟ੖ᴹᆹޘ ˅⭘শਢᱟ䴰㾱㘳㲁Ⲵањ䟽㾱ഐ㍐Ǆ↔ཆˈṩᦞഭ䱵伏૱⌅ިငઈՊ˄Codex֯ ᆹޘ䇴ԧᤷই˄Codex Alimentarius, 2003; Codex Alimentarius, 2009˅ˈ㳻ⲭ䍘Ⲵ▌ ൘∂ᙗ䇴ԧ䴰㾱∄䖳ᯠᕅޕ㳻ⲭоᐢ⸕∂㍐઼ᣇ㩕ޫഐᆀⲴ⭏ॆ⢩ᙗᱟ੖ާᴹ ⴨լᙗˈ䇴ԧⲴ⭏⢙ॆᆖ⢩ᙗवᤜ˖1˅䈕㳻ⲭоᐢ⸕∂㳻ⲭ઼ᣇ㩕ޫഐᆀᡆަ ᓧާᴹн㢟ᖡ૽Ⲵ⭏⢙⍫ᙗ㳻ⲭᱟ੖ާᴹᒿࡇ⴨լᙗ˗2˅䈕ڕ⢙ᆳሩӪ㊫৺ࣘ 㳻ⲭ൘ପңࣘ⢙⎸ॆ⏢ѝⲴ⎸ॆᙗ˗3˅䈕㳻ⲭᱟ੖ާᴹ✝っᇊᙗ˗4˅䈕㳻ⲭሩ ପңࣘ⢙ᱟ੖ާᴹᙕᙗ㓿ਓ∂ᙗ˗5˅䈕㳻ⲭ൘֌⢙ਟ伏⭘䜘࠶ѝᡰঐ∄ֻԕ৺ ሩӪ㊫Ⲵ㟣伏᳤䵢≤ᒣǄ ṩᦞԕкḷ߶ሩ CP4 EPSPS 㳻ⲭԕ৺ MON 87427 ⦹㊣䘋㹼Ҷ▌൘∂ᙗ䇴ԧˈ 㔃᷌㺘᰾ሩӪ㊫઼ପңࣘ⢙нާᴹ∂ᙗ仾䲙Ǆ

81 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᙗޘCP4 EPSPS 㳻ⲭ׋փ⭏⢙Ⲵᆹ˅1

CP4 EPSPS 㳻ⲭⲴ׋փ⭏⢙Ѫ൏༔ṩⱼߌᵶ㧼 CP4 㧼ṚˈഐѪᆳ㜭ӗ⭏ཙ Ⲵǃо㥹⭈㟖Ӣ઼ᙗվⲴ EPSPS 㳻ⲭ㘼֯㺘䗮 CP4 EPSPS 㳻ⲭⲴἽ⢙ਟԕᣇ❦ 㥹⭈㟖˄Padgette et al., 1996˅Ǆ㖾ഭᗞ⭏⢙ษޫᓃ˄American Type Culture Collection˅ᐢ㓿ሩ䘉⿽ CP4 㧼Ṛ䘋㹼Ҷ࠶⿫઼䢤ᇊˈᴰ㓸⺞ᇊ኎Ҿ൏༔ߌᵶ㧼 Ⲵ⭏⨶ሿ⿽Ǆⴞࡽ䘈⋑ᴹᣕ䚃㺘᰾ԫօа⿽ߌᵶ㧼ሩӪ㊫ᡆ㘵ࣘ⢙ާᴹ㠤⯵㜭 ࣋ˈ㘼фߌᵶ㧼а㡜нᱟ㠤᭿Ⓚ˄FAO/WHO, 1991˅Ǆ↔ཆˈṩᦞ FAO/WHO˄2001˅ ᣕ੺ˈⴞࡽ䘈⋑ਁ⧠ԫօⲴӪ㗔ሩ㓶㧼ᙗ㳻ⲭ䗷᭿Ǆ

2˅CP4 EPSPS 㳻ⲭⲴ⭏ॆ⢩ᙗ৺㳻ⲭㅹ਼ᙗ࠶᷀

CP4 EPSPSᱟ⭡455њ≘ส䞨㓴ᡀⲴঅ䬮ཊ㛭ˈ⨶䇪࠶ᆀ䟿Ѫ47.6 kDaǄӾ ᒿ ࡇкⴻˈCP4 EPSPSоἽ⢙ᶕⓀⲴEPSPSާᴹ25-30%Ⲵ⴨լᙗˈо㓶㧼ᶕⓀⲴ EPSPSާᴹ50-90%Ⲵ⴨լᙗǄⴞࡽᐢ⸕ⲴᡰᴹᶕⓀEPSPS䞦˄वᤜCP4 EPSPS˅ ൘й㓗ᤃᢁ㔃ᶴк儈ᓖа㠤˄Hammond, et al. 2013˅ǄCP4 EPSPS䞦Ⲵ⤜⢩ᶴ䙐 ᤱᖸ儈ⲴӢ઼ᙗǄ؍ᗇަሩ㥹⭈㟖н᭿ᝏˈն਼ᰦሩཙ❦ᓅ⢙PEP֯

EPSPS 䞦˄वᤜ CP4 EPSPS˅ާᴹ儈ᓖⲴᓅ⢙⢩ᔲᙗǄⴞࡽᐢ⸕ሩ EPSPS ਈॆǄ׳䞦ާᴹ⭏⢙ᆖ᜿ѹⲴᓅ⢙ӵѪ S3P ઼ PEPǄ㥹⭈㟖н㜭ਁ⭏ EPSPS 䞦㧭㥹䞨оབྷ㛐ᵶ㧼ᶕⓀ EPSPS 䞦Ⲵ㔃ਸ㜭࣋䶎ᑨᕡˈо S3P ⴨∄ˈ㧭㥹䞨䴰㾱 ˈ䶎ᑨ儈⎃ᓖ˄mM 㓗˅᡽ᴹਟ㜭㻛 EPSPS 䞦ۜॆ˄Gruys, et al. 1992˅Ǆ⹄ウ㺘᰾ Ἵ⢙փ޵ཙ❦ᆈ൘ S3P ㊫լ⢙ྲ⭢ส㧭㥹䞨ǃཾቬ䞨ǃҼ≒㧭㥹䞨䜭нᱟ EPSPS Ⲵᓅ⢙˄Franz, et al. 1997˅Ǆ

CP4 EPSPS㳻ⲭⲴ䞦⍫ᙗ઼っᇊᙗਇpH٬ǃ⑙ᓖ઼⿫ᆀᕪᓖㅹഐ㍐ᖡ૽Ǆᴰ 䘲䞦⍫pHѪ9-9.5ˈpH٬䗷儈ᡆ䗷վ䜭Պ䱽վ䞦⍫ᙗˈpHሿҾ5.5ᰦCP4 EPSPS䞦 ⍫ࠐѾᆼޘїཡˈн䗷⭡pHᕅ䎧Ⲵ䞦⍫ਈॆᒦ䶎нਟ䘶˄Padgette et al. 1993˅Ǆ CP4 EPSPSሩ✝нっᇊˈ⑙ᓖ䎵䗷75°CᰦˈCP4 EPSPSⲴ࣏㜭⍫ᙗণ䱽㠣Ự⍻䲀 ԕлˈն࣐✝ᒦнᖡ૽㳻ⲭа㓗㔃ᶴⲴᆼᮤᙗǄ䘉ㅖਸ✝ࣘ࣋ᆖ⌅ࡉˈণ⑙ᓖॷ ˈ儈Պ֯㳻ⲭ儈㓗㔃ᶴਈᙗˈᕅ䎧䞦⍫ᙗїཡǄCP4 EPSPSሩ✝нっᇊⲴ⢩ᙗ㺘᰾ ൘ᐕъ࣐ᐕᶑԦлˈᴰ㓸Ⲵ䖜สഐ⦹㊣ӗ૱ѝнཚਟ㜭ᆈ൘ާᴹ࣏㜭⍫ᙗⲴ CP4 EPSPS㳻ⲭ˄Hammond and Jez 2011˅ǄоᡰᴹިරⲴ㳻ⲭ䞦㊫аṧˈ CP4 EPSPSⲴۜॆ⍫ᙗ䘈ਇ৽ᓄⓦ⏢ѝ⿫ᆀᕪᓖⲴᖡ૽Ǆ↓ᑨⲴ䍞ᆈ㕃ߢ⏢˄1 mM KCl˅ᶑԦлˈCP4 EPSPSⲴ⢩ᔲ⍫ᙗᴰ儈㜭ཏ䗮ࡠ5.5 U/mgǄCP4 EPSPS Ⲵ⢩ᔲ⍫ᙗ䲿⵰⿫ᆀᕪᓖ໎བྷ㘼ॷ儈ˈ㠣50 mM KClᰦˈ䞦⍫ᙗ㜭ཏ໎࣐2-2.6 ؽ˄Padgette et al. 1993˅Ǆ

⼧㔬ਸ֯⭘ㅹ⭥⛩⊹䱽ǃ⺛䞨䬥࠶㓗࠶⿫ǃ⮿≤ᙗቲ᷀ǃ䱤⿫ᆀӔᦒቲ઼᷀ 䞨㓔㔤㍐Ӣ઼ቲ᷀ㅹᯩ⌅ˈӾMON 87427 ⿽ᆀⲴ⹄⼘ᨀਆ⢙ѝ㓟ॆᗇࡠ CP4 EPSPS㳻ⲭ˄䈖㿱䲿⭣䈧ҖᨀӔⲴᢰᵟᣕ੺5˅Ǆ䟷⭘ཊ⿽࠶᷀ᢰᵟሩ㓟ॆⲴ Ἵ⢙ⓀCP4 EPSPS㳻ⲭ䘋㹼⭏ॆ⢩ᙗ䢤ᇊˈᡰ⭘࠶᷀ᢰᵟवᤜ˖1˅⭘Bio-Rad㳻 ⲭ࠶᷀㌫㔏⍻ᇊᙫ㳻ⲭ⎃ᓖ˗2˅ॱҼ✧ส⼪䞨䫐-㚊щ✟䞠㜪ࠍ㜦⭥⌣

82 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 SDS-PAGE˅৺ᇶᓖ䇑䟿˗3˅N-ᵛㄟᒿࡇ࠶᷀˗4˅ส䍘䖵ࣙ◰ݹ䀓᷀⭥⿫伎˄ 㹼ᰦ䰤˄MALDI-TOF˅䍘䉡˗5˅ݽ⯛ঠ䘩઼ᇶᓖ䇑⍻ᇊ࠶᷀˗6˅CP4 EPSPS 䞦⍫ᙗỰ⍻˗7˅㌆สॆ࠶᷀Ǆ↔ཆˈ䘈䙊䗷SDS-PAGEՠ㇇н਼⑙ᓖл䍞ᆈа ⇥ᰦ䰤ਾޘ䮯CP4 EPSPS 㳻ⲭⲴ㓟ᓖ৺࠶ᆀ䟿ˈӾ㘼䇴ՠ㓟ॆⲴἽ⢙Ⓚ CP4 EPSPS㳻ⲭⲴっᇊᙗǄᒦ਼ᰦоབྷ㛐ᵶ㧼㺘䗮㌫㔏ѝ㓟ॆᗇࡠⲴCP4 EPSPS 㳻ⲭ䘋㹼∄䖳Ǆ

䟷⭘ᣇ-CP4 EPSPS ᣇփݽ⯛ঠ䘩࠶᷀ǃN-ᵛㄟ⍻ᒿ઼ MALDI-TOF 䍘䉡࠶ ᷀ᢰᵟሩ㜠㳻ⲭ䞦⎸ॆⲴ CP4 EPSPS 㳻ⲭ䘋㹼࠶᷀ˈԕ傼䇱 MON 87427 ѝ CP4 EPSPS 㳻ⲭⲴ䓛ԭǄ⭘ Bio-Rad 㳻ⲭ࠶᷀㌫㔏⍻ᗇ MON 87427 ⿽ᆀѝ㓟ॆ ᗇࡠⲴ CP4 EPSPS Ⲵᙫ㳻ⲭ⎃ᓖѪ 0.1 mg/ml˗䙊䗷㘳傜ᯟӞ㬍 G ḃ㢢 SDS 㚊 щ✟䞠㜪ࠍ㜦Ⲵᇶᓖ࠶᷀⍻ᇊᗇࡠ MON 87427 ޘ䮯 CP4 EPSPS 㳻ⲭⲴ㓟ᓖ઼࠶ ᆀ䟿࠶࡛Ѫ 96%઼ 44.1 kDaǄ˗N-ᵛㄟᒿࡇ࠶᷀ˈ⺞䇔Ҷ䎧࿻Ⲵ 15 њ≘ส䞨ˈ䞦䀓㛭⇥㾶ⴆമ䉡㾶ⴆҶ亴ᵏ㳻ⲭᒿࡇⲴ 70.3%˗㓿㜠㳻ⲭ䞦⎸ॆⲴ CP4 EPSPS 㳻ⲭ䘋㹼 MALDI-TOF 䍘䉡࠶᷀ˈӗ⭏Ⲵཊ㛭ᒣ൷䍘䟿Ѫ 47552 Daˈо MON 87427 ѝ cp4 epsps Ṩ㤧䞨ᒿࡇ᧘ሬࠪⲴ CP4 EPSPS 㳻ⲭⲴ⨶䇪㜠㳻ⲭ䞦⎸ ॆ⡷⇥ᱟа㠤ⲴǄ

䙊䗷ݽ⯛⍫ᙗǃ࠶ᆀ䟿⍻ᇊǃ䞦⍫⍻䈅ǃ㌆สॆ⣦ᘱㅹ࠶᷀ˈ䘋а↕⺞ᇊ CP4 EPSPS 㳻ⲭⲴ⭏ॆ⢩ᙗˈᒦ䇱ᇎབྷ㛐ᵶ㧼ᶕⓀ㳻ⲭо MON 87427 ѝ㓟ॆᗇ ࡠ CP4 EPSPS 㳻ⲭⲴㅹ਼ᙗǄሩ MON 87427 ᶕⓀ઼བྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭⲴؑਧᕪᓖ䘋㹼ݹᇶᓖ࠶᷀ˈ᨝⽪є㘵ᒣ൷٬ⲴᐞᔲѪ 9.6%ˈ൘䇮ᇊⲴ᧕ ਇḷ߶±35%ԕ޵˗㘳傜ᯟӞ㬍ḃ㢢Ⲵ SDS-PAGE ⭥⌣࠶᷀㺘᰾ˈMON 87427 ᶕⓀ CP4 EPSPS 㳻ⲭ㺘㿲࠶ᆀ䟿Ѫ 44.1 kDaˈоབྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS ৲ ➗ḷ߶㳻ⲭާᴹ⴨਼Ⲵ䗱〫ս㖞˗䙊䗷⼧䞨䟺᭮ᇎ傼ᶕ⍻ᇊ CP4 EPSPS 㳻ⲭⲴ MON 87427 ᶕⓀ઼བྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ⢩ᔲ⍫ᙗ࠶ˈᔲ䞦⍫ᙗ٬⢩ ࡛Ѫ 8.67 U/mg ઼ 5.41 U/mg˗㓿䢤ᇊˈMON 87427 ᶕⓀⲴ CP4 EPSPS 㳻ⲭ⋑ᴹ 㻛㌆สॆǄ㔬ਸк䘠㔃᷌ˈMON 87427 ᶕⓀ઼བྷ㛐ᵶ㧼ӗ⭏ CP4 EPSPS 㳻ⲭᱟ ᆈㅜ 14 ཙᰦ˅㳻؍ᇎ䍘ㅹ਼ⲴǄ↔ཆˈ䙊䗷∄䖳ᇎ傼ᔰ࿻઼㔃ᶏᰦ˄-80ćߠ㇡ ⲭⲴ㓟ᓖ઼࠶ᆀ䟿⍻ᇊ㔃᷌ˈ䇔Ѫ MON 87427 ᨀਆⲴ CP4 EPSPS 㳻ⲭ൘ᇎ傼䗷 ᤱっᇊǄ؍〻ѝ

3˅CP4 EPSPS 㳻ⲭⲴ䮯ᵏᆹޘᓄ⭘শਢ

EPSPS 㳻ⲭ˄5-✟䞷щ䞞䞠-3-⼧䞨䖜〫䞦˅ᒯ⌋ᆈ൘ҾἽ⢙઼ᗞ⭏⢙ѝˈ䇨 ཊ⹄ウ䜭ӾἽ⢙઼ᗞ⭏⢙ѝ࠶⿫ࠪ EPSPS˄Harrison et al., 1996; Haslam, 1993; Klee et al., 1987; Schönbrunn et al., 2001; Steinrücken and Amrhein, 1984˅ˈ৲о㧭㥹䞨ԓ䉒䙄ᖴǄቭ㇑ପңࣘ⢙ǃ劬㊫ǃ呏㊫ǃ⡜㹼㊫ࣘ⢙ԕ৺ᰶ㲛փ޵нᆈ൘㧭㥹䞨ԓ䉒䙄ᖴ˄Alibhai and Stallings; 2001˅ˈҏ⋑ᴹ EPSPS 㳻ⲭˈնԆԜⲴ伏⢙ ѝ EPSPS 䶎ᑨᲞ䙽Ǆ਴㊫ᶕⓀⲴ EPSPS 㳻ⲭⲴ㕆⸱สഐᐢ㓿㻛ݻ䲶ࠪᶕˈ䘉Ӌ ᆸ˄Padgette et al., 1996˅Ǆ㓶㧼Ⲵ؍㳻ⲭⲴۜॆ㔃ᶴฏ˄catalytic domain˅儈ᓖ EPSPS 㳻ⲭй㔤㔃ᶴ˄Stallings et al., 1991˅ԕ৺䞦઼ࣘ࣋ॆᆖ৽ᓄᵪࡦ˄Anderson

83 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 and Johnson, 1990˅䜭ᐢ㓿ᖸ␵ᾊǄMON 87427 㺘䗮Ⲵ CP4 EPSPS 㳻ⲭᶕⓀҾ ൏༔ߌᵶ㧼 CP4 㧼Ṛˈᱟ䇨ཊ㠚❦⭼ᒯ⌋ᆈ൘Ⲵ EPSPS 㳻ⲭѝⲴа⿽Ǆ䲔Ҷሩ 㥹⭈㟖Ⲵ㙀ਇᙗн਼ཆˈоἽ⢙ѝⲴ EPSPS 㳻ⲭ൘࣏㜭кᇎ䍘ㅹ਼˄Padgette et al., 1996˅Ǆ

CP4 EPSPS 㳻ⲭާᴹ൘ޘ⨳伏⢙䬮ѝ䮯ᵏᆹޘ⎸䍩ⲴশਢǄ㠚Ӿ 1996 ᒤᣇ 㥹⭈㟖བྷ䉶୶ъॆ⿽Ἵԕᶕˈਜ਼ᴹ CP4 EPSPS 㳻ⲭⲴߌӗ૱ቡаⴤ൘ޘ⨳伏⢙ 伏⭘Ǆਜ਼ᴹ CP4 EPSPS Ⲵᣇ㥹⭈㟖֌⢙⇿ᒤޘ׋ᓄ䬮ѝᆈ൘ᒦ㻛Ӫ㊫઼ࣘ⢙ᆹ 䜭ᴹ 30 ཊњӗ૱൘ 13 њഭᇦ⿽Ἵ䶒〟䎵䗷 7400 зޜ产˄Dill et al., 2008˅Ǆц ⭼㤳ത޵ᐢ㓿ᴹ䇨ཊ㺘䗮 CP4 EPSPS 㳻ⲭⲴᣇ㥹⭈㟖֌⢙˄བྷ䉶ǃ⦹㊣ǃ⋩㨌ǃ ỹ㣡ǃ⭌㨌઼㤌㬯ㅹ˅㧧߶୶ъॆ⿽Ἵˈҏ㻛वᤜѝഭ൘޵Ⲵ䇨ཊഭᇦ䇨ਟ䘋ਓ ⭘֌࣐ᐕ৏ᯉǄMON 87427 㺘䗮Ⲵ CP4 EPSPS 㳻ⲭо䘉Ӌᐢ㓿㧧ᗇᢩ߶ᒦ୶ъ ᣔ㖢؍ᓄ⭘䘁 20 ᒤⲴᣇ㥹⭈㟖֌⢙ѝⲴ CP4 EPSPS ᱟ਼ṧⲴǄ㖾ഭ⧟ຳॆ ˄US-EPA˅ҏᇑḕҶ CP4 EPSPS 㳻ⲭⲴᆹޘᙗˈṩᦞަ൘⁑ᤏ㛳⏢⎸ॆǃоᐢ ⸕∂㍐઼ᣇ㩕ޫഐᆀǃ㠤᭿㳻ⲭⲴ⭏⢙ؑ᚟ᆖ࠶᷀ǃᙕᙗ㓿ਓ∂ᙗᇎ傼ㅹ㔃઼᷌ U.S. EPA, 1996˅Ǆ˄Ⲵˈн䴰㾱൘ߌӗ૱ѝᔪ・ަਜ਼䟿Ⲵᇩ䇨䰸٬ޘᮠᦞˈ䇔Ѫᱟᆹ

㠚 1996 ᒤᣇ㥹⭈㟖֌⢙୶ъॆ⿽Ἵԕᶕˈᐢᴹ䎵䗷 1000 ӯཤࣘ⢙㻛侢லਜ਼ ᓧ઼⭏ӗ⦷Ⲵڕ⢙ᴹ䖜สഐ֌⢙˄བྷ䜘࠶ᱟᣇ㥹⭈㟖֌⢙˅Ⲵ侢ᯉˈ⋑ᴹᖡ૽ࣘ ᣕ੺Ǆаㇷ㓿䗷਼㹼䇴䇞ⲴޣҾ侢லਜ਼ᴹ䖜สഐ֌⢙˄वᤜᣇ㥹⭈㟖֌⢙˅侢ᯉ ᓧᖡ૽Ⲵ᮷⥞㔬䘠˄Eenennaam & Young, 2014˅ҏᤷࠪˈⴞࡽڕሩࣘ⢙⭏䮯઼ ᓧӗ⭏н࡙ᖡ૽Ǆ൘ڕ䘈⋑ᴹԫօֻᆀ㺘᰾ਜ਼ᴹ䖜สഐ֌⢙侢ᯉՊሩࣘ⢙⭏䮯઼ 䗷৫ 20 ᒤˈޘ⨳ᐢ㓿ᴹ 488 亩⅑ޣҾ CP4 EPSPS 㳻ⲭᆹޘᙗⲴᇑḕᡆ䇴ᇑˈᗇ ᓧ⋑ᴹн㢟ᖡ૽Ǆڕ⢙ࠪⲴ㔃䇪൷䇔Ѫ CP4 EPSPS 㳻ⲭሩӪ㊫઼ࣘ

4˅CP4 EPSPS оަᆳᐢ⸕∂㳻ⲭⲴ㔃ᶴ⴨լᙗ

㻛䇴ՠ㳻ⲭоᐢ⸕∂㍐ᡆа؍⺞㳻ⲭ▌൘∂ᙗⲴ⭏⢙ؑ᚟ᆖ࠶᷀ⲴⴞⲴᱟ Ӌᣇ㩕ޫ㳻ⲭ൘ᒿࡇ઼㔃ᶴкнާᴹ⴨լᙗǄ࡙⭘ FASTA ᒿࡇ࠶᷀䖟Ԧˈਟԕ ࠶᷀ CP4 EPSPS 㳻ⲭо∂㍐㳻ⲭᮠᦞᓃѝ਴⿽㳻ⲭ㔃ᶴⲴ⴨լᙗ˄䈖㿱䲿⭣䈧 ҖᨀӔⲴᢰᵟᣕ੺ 6˅ǄFASTA ㇇⌅䲔Ҷ㜭ⴤ᧕ሩ∄਴⿽㳻ⲭ䍘Ⲵ≘ส䞨ᒿࡇ˄ণ ࡍ㓗㳻ⲭ㔃ᶴ˅ཆˈ䘈㜭⭘Ҿ᧘ሬᴤ儈а㓗Ⲵ㔃ᶴ⴨լᙗ˄ণҼ㓗઼й㓗㳻ⲭ㔃 ᶴ˅Ǆ൘ᮤњⲴᒿࡇ䟼ᴹ儈ᓖ⴨լᙗⲴ㳻ⲭˈᑨᑨᱟ਼Ⓚ㳻ⲭˈ㘼਼Ⓚ㳻ⲭ㓿ᑨ ٬䴰㾱ሿҾᡆㅹҾ 1e-5 ᴹ਼ṧⲴҼ㓗઼й㓗㔃ᶴǄа㡜㘼䀰ˈєњᒿࡇ䰤Ⲵ Eާ ᡽㻛䇔Ѫᴹᱮ㪇਼ⓀᙗǄѪҶ䘋㹼ᴤޘ䶒ൠ䇴ԧˈӾ 2016 ᒤᔰ࿻ˈ䟷⭘ањᶱ E=0.01 䘋㹼⭏⢙ؑ᚟ᆖ࠶᷀Ǆ ᆸⲴ䰸٬؍ㄟ

䪸ሩ MON 87427 Ⲵ⭏⢙ؑ᚟ᆖ࠶᷀䟷⭘Ⲵ∂㍐㳻ⲭᮠᦞᓃѪ TOX_2016Ǆ TOX_2016 ᮠᦞᓃᱟӾ PRT_2016 ᮠᦞᓃѝ䗷└䲔৫ਟ㜭Ⲵ䶎∂㍐㳻ⲭਾᖒᡀⲴ ӊᮠᦞᓃǄTOX_2016 वᤜҶ 18,554 њ㳻ⲭᒿࡇǄ⭘ CP4 EPSPS 㳻ⲭᒿࡇ൘ ٬Ѫ 0.51Ǆ-TOX_2016 ᮠᦞᓃѝ䘋㹼ᩌ㍒ˈ䇴࠶ᴰ儈Ⲵ∄ሩѪ GI-406373209ˈަ E ٬Ѫ 0.01 ᰦˈ⋑ᴹᗇࡠ ᆸ E؍٬䗷儈ˈ∄ሩ㔃᷌нާᴹ᭸᜿ѹǄ䟷⭘-⭡Ҿަ E

84 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ԫօ∄ሩ㔃᷌˄ᢰᵟᣕ੺ 6˅Ǆഐ↔ਟԕᗇࠪ㔃䇪䇔Ѫ MON 87427 о TOX_2016 ᮠᦞᓃѝⲴ∂㍐㳻ⲭ䜭нާᴹᒿࡇ઼㔃ᶴкⲴ⴨լᙗǄ

ਖཆˈ䘈⭘ CP4 EPSPS 㳻ⲭᒿࡇ൘ PRT_2016 ᮠᦞᓃ˄ᶕⓀҾ NCBI 㖁ㄉл 䖭Ⲵ GenBank 㳻ⲭᮠᦞᓃ 211 ᴤᯠ⡸ᵜˈवਜ਼ᴹ 49,462,283 њާᴹ⭏⢙⍫ᙗⲴ 㳻ⲭᒿࡇ˅ѝ䘋㹼ᩌ㍒ˈ䇴࠶ᴰ儈Ⲵ∄ሩѪ൘ 455 њ≘ส䞨㤳ത޵ާᴹ 100.00% ٬Ѫ 6.9e-173Ǆ䘉а㔃᷌ӵ-⴨լᙗⲴᒿࡇѪ CP4 EPSPS˄GI-347666444˅ˈަ E ᓧⲴڕ䇱ᇎҶ CP4 EPSPS 㳻ⲭⲴ䓛ԭˈн㜭㺘᰾ CP4 EPSPS 㳻ⲭᵜ䓛ާᴹডᇣ 0.01 䘋㹼ㆋ䘹ᰦˈᗇࡠҶᴤཊⲴ∄ሩ㔃᷌ˈަѝབྷ䜘=٬ ᆸ E؍⭏⢙⍫ᙗǄ䟷⭘ ࠶઼ EPSPS 㳻ⲭа㠤Ǆ∄ሩ㔃᷌䇶࡛ࠪᶕ㠚ߌᵶ㧼 CP4 㧼ṚⲴ CP4 EPSPS о઼ ᶕ㠚ަԆ⢙⿽ EPSPS 㳻ⲭⲴ⴨լᙗн㜭㺘᰾ CP4 EPSPS 㳻ⲭᆈ൘▌൘н㢟⭏⢙ ▌⍫ᙗǄഐ↔ˈ֯⭘ PRT_2016 ᮠᦞᓃ⋑ᴹỰ㍒㔃᷌㺘᰾ CP4 EPSPS 㳻ⲭᆈ൘ ൘Ⲵᣇ㩕ޫഐᆀ⍫ᙗǄ 5˅CP4 EPSPS 㳻ⲭփཆ⁑ᤏ⎸ॆᇎ傼 Harrison ㅹ˄ 1996˅䇱᰾Ҷ CP4 EPSPS 㳻ⲭ㜭൘⁑ᤏ⎸ॆᶑԦлՊ㻛䗵䙏⎸ ॆᦹǄṩᦞ Western ঠ䘩࠶᷀ˈCP4 EPSPS ൘⁑ᤏ㛳⏢⎸ॆ㌫㔏ѝ 15 s ਾቡᰐ⌅ Ự⍻ࡠǄ ᆏኡ䜭ޜਨሩ CP4 EPSPS 㳻ⲭ൘⿫փ⁑ᤏ㛳⏢˄SGF˅⎸ॆᇎ傼ҏ䇱ᇎҶ к䘠㿲⛩Ǆ䈕䈅傼䟷⭘ഭ䱵⭏ભ、ᆖ⹄ウᡰ˄ILSI˅ޜᐳⲴḷ߶ᯩ⌅˄Thomas et al., 2005˅Ǆ਼ Harrison ㅹ˄1996˅ԕࡽⲴᇎ傼аṧˈᵜ亩⹄ウҏ䟷⭘Ҷབྷ㛐ᵶ㧼 ⭏ӗⲴ CP4 EPSPS 㳻ⲭˈ⎸ॆ⦷䟷⭘ SDS-PAGE ⭥⌣ࠍ㜦ḃ㢢ǃ㳻ⲭ䍘ঠ䘩઼ CP4 EPSPS 䞦৽ᓄ⍫ᙗ䢤ᇊ䘋㹼䇴ՠǄ ᇎ傼㔃᷌䇱ᇎҶ CP4 EPSPS 㳻ⲭ൘փཆ⁑ᤏⲴ SGF 䟼䗵䙏㻛⎸ॆǄ SDS-PAGE ࠍ㜦ḃ㢢㔃᷌㺘᰾ˈ98%ԕкⲴ CP4 EPSPS 㳻ⲭ൘ 15 s ޵㻛䗵䙏⎸ ॆ˄മ 21˅ǄWestern ᵲӔ࠶᷀㔃᷌㺘᰾ˈ95%ԕкⲴ CP4 EPSPS 㳻ⲭ൘ 15 s ޵ ণᰐ⌅Ự⍻˄മ 22˅Ǆ਼ṧˈCP4 EPSPS 㳻ⲭ൘ SGF 䟼ษޫнࡠ 15 sˈަ⍫ᙗ䱽 վࡠ 10%ԕл˄㺘 18˅Ǆᙫѻˈփཆ⁑ᤏ⎸ॆᇎ傼Ⲵ㔃᷌ਟԕᯝᇊ CP4 EPSPS ᓧӗ⭏ড䲙Ǆڕ㳻ⲭ㜭൘⁑ᤏ㛳⏢䟼䗵䙏㻛䱽䀓ˈᡰԕнਟ㜭ሩӪփ 6˅CP4 EPSPS 㳻ⲭⲴ✝っᇊᙗᇎ傼 ࣐✝༴⨶൘⦹㊣㊭㋂ԕ৺ਜ਼ᴹ⦹㊣㊭㋂Ⲵӗ૱࣐ᐕ䗷〻ѝᓄ⭘䶎ᑨᒯ⌋ˈഐ ↔䇴ՠ CP4 EPSPS 㳻ⲭሩ✝༴⨶Ⲵっᇊᙗҏᱟ䶎ᑨ䟽㾱ⲴǄ ✝༴⨶ᇎ傼⭘Ⲵ CP4 EPSPS ᱟ⭡བྷ㛐ᵶ㧼⭏ӗⲴˈ✝༴⨶⑙ᓖѪ 25°Cǃ37°Cǃ 55°Cǃ75°C ઼ 95°Cˈ༴⨶ᰦ䰤Ѫ 15 min ᡆ㘵 30 minǄ䇴ԧⲴ޵ᇩवᤜ˖1˅࣐ ✝༴⨶ሩ CP4 EPSPS 㳻ⲭ䞦⍫࣋Ⲵᖡ૽˗2˅࣐✝༴⨶ሩ㳻ⲭᆼᮤᙗⲴᖡ૽Ǆ ൘ 25°Cǃ37°Cǃ55°Cǃ75°C ઼ 95°C ⑙ᓖᶑԦл࣐✝༴⨶ 15 ઼ 30 min ਾˈ CP4 EPSPS 㳻ⲭⲴ⍫ᙗ⍻䈅㔃᷌࠶࡛㿱㺘 19 ઼㺘 20Ǆᖃԕ 25ºCǃ37ºC ઼ 55ºC ࣐✝ 15 min ਾ㿲ሏࡠ CP4 EPSPS ⍫ᙗ〽ᴹ䱽վˈ࠶࡛䱽㠣ሩ➗Ⲵ 81%ǃ84%઼ 70%˗ն࣐✝ 30 min ਾˈ25ºC ༴⨶Ⲵ CP4 EPSPS ⍫ᙗ⋑ᴹਈॆˈ37ºC ༴⨶㓴Ⲵ 85 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䞦⍫䱽㠣ሩ➗ⲴⲴ 88%ˈ㘼 55ºC ༴⨶㓴Ⲵ CP4 EPSPS 㺘⧠Ѫབྷᑵл䱽ˈӵѪሩ ➗Ⲵ 25%Ǆн䇪ᱟ༴⨶ 15 min 䘈ᱟ༴⨶ 30 minˈ75°C ઼ 95°C ⑙ᓖᶑԦлⲴ CP4 EPSPS 䞦⍫൷վҾỰ⍻䲀Ǆ SDS-PAGE ࠶᷀㔃᷌㺘᰾ˈ৲➗ḷ߶઼ሩ➗⢙वਜ਼аᶑ㺘㿲࠶ᆀ䟿㓖Ѫ 43.8 kDa ⲴѫᶑᑖˈሩᓄҾ CP4 EPSPS 㳻ⲭ˄മ 23 ઼മ 24˅Ǆ൘ᡰᴹ༴⨶ᰦ䰤 ⌣ˈ༴⨶⑙ᓖᶑԦлˈṧ૱Ⲵ 43.8 kDa ᶑᑖؑਧᕪᓖ⋑ᴹ᰾ᱮⲴ䱽վ˄മ 23઼ 䚃 3~7˗മ 24ˈ⌣䚃 2~7˅Ǆ к䘠㔃᷌㺘᰾ˈ࣐✝༴⨶ӵՊ֯ CP4 EPSPS 㳻ⲭⲴ䞦⍫ᙗ䱽վˈնнՊ֯ CP4 EPSPS 㳻ⲭ䱽䀓Ǆഐ↔ˈнՊᖒᡀᯠⲴ㳻ⲭ⡷⇥㘼ᑖᶕᯠⲴ▌൘㠤᭿仾䲙Ǆ

86 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 བྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ

㛳㳻ⲭ䞦

മ21. SDS-PAGEࠍ㜦⭥⌣࠶᷀˖CP4 EPSPS㳻ⲭ൘⁑ᤏ㛳⏢˄SGF˅ѝⲴ⎸ॆ 䟷⭘ 10-20%㚊щ✟䞠㜪ࠍ㜦⭥⌣˄SDS-PAGE˅࠶⿫㳻ⲭˈ㕃ߢ⏢Ѫ tricine 㕃ߢ⏢ˈ㜦փӞ㬍 Gḃ㢢ǄสҾ⎸ॆࡽⲴ⎃ᓖˈ⇿⌣䚃кṧ CP4 EPSPS 㳻ⲭ 500 ngǄ

⌣䚃 ṧ૱᧿䘠 ⎸ॆᰦ䰤 1 ࠶ᆀ䟿ḷ䇠 2 ᰐ㛳㳻ⲭ䞦Ⲵᇎ傼ሩ➗˄P0˅ 0s 3 ᰐ CP4 EPSPS Ⲵᇎ傼ሩ➗˄N0˅ 0s 4 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=0 0s 5 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=1 15s 6 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=2 30s 7 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=3 1min 8 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=4 2min 9 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=5 4min 10 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=6 8min 11 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=7 15min 12 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=8 30min 13 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=9 60min 14 ᰐ CP4 EPSPS Ⲵᇎ傼ሩ➗˄N9˅ 60 min 15 ᰐ㛳㳻ⲭ䞦Ⲵᇎ傼ሩ➗˄P9˅ 60 min

87 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 བྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ

മ22. Western㳻ⲭ䍘ঠ䘩࠶᷀˖CP4 EPSPS㳻ⲭ൘⁑ᤏ㛳⏢˄SGF˅ѝⲴ⎸ॆ 䟷⭘ 10-20%㚊щ✟䞠㜪ởᓖࠍ㜦˄SDS-PAGE˅࠶⿫㳻ⲭǄสҾ⎸ॆࡽⲴ⎃ ᓖˈ⇿⌣䚃кṧ CP4 EPSPS 㳻ⲭ 1ngǄ

⌣䚃 ṧ૱᧿䘠 ⎸ॆᰦ䰤 1 ࠶ᆀ䟿ḷ䇠 2 ᰐ㛳㳻ⲭ䞦Ⲵᇎ傼ሩ➗˄P0˅ 0s 3 ᰐ CP4 EPSPS Ⲵᇎ傼ሩ➗˄N0˅ 0s 4 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=0 0s 5 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=1 15s 6 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=2 30s 7 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=3 1min 8 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=4 2min 9 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=5 4min 10 ൘ SGF 䟼Ⲵ CPu4 EPSPS 㳻ⲭˈT=6 8min 11 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=7 15min 12 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=8 30min 13 ൘ SGF 䟼Ⲵ CP4 EPSPS 㳻ⲭˈT=9 60min 14 ᰐ CP4 EPSPS Ⲵᇎ傼ሩ➗˄N9˅ 60 min 15 ᰐ㛳㳻ⲭ䞦Ⲵᇎ傼ሩ➗˄P9˅ 60 min

88 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ 23. ࣐✝༴⨶ 15 ࠶䫏ਾ CP4 EPSPS Ⲵ SDS-PAGE Ự⍻ ࣐✝༴⨶ਾⲴ CP4 EPSPS ൘ Tris-⭈≘䞨 4-20%㚊щ✟䞠㜪ࠍ㜦⭥⌣к࠶ ⿫˄3.2 μg ᙫ㳻ⲭ˅ˈ㜦փӞ㬍 G ḃ㢢Ǆ࠶ᆀ䟿অսѪ˄kDa˅Ǆ

⌣䚃 ṧ૱઼༴⨶᧿䘠㳻ⲭ䟿˄μg˅

1 ᇭ㤳ത࠶ᆀ䟿ḷ䇠 4.5 2 CP4 EPSPS Ѫ࣐✝༴⨶˄0ºC˅ 3.2 3 CP4 EPSPS 25 ºC ✝༴⨶ 3.2 4 CP4 EPSPS 37 ºC ✝༴⨶ 3.2 5 CP4 EPSPS 55 ºC ✝༴⨶ 3.2 6 CP4 EPSPS 75 ºC ✝༴⨶ 3.2 7 CP4 EPSPS 95 ºC ✝༴⨶ 3.2 8 ḷ߶ CP4 EPSPS 㳻ⲭ 3.2 9 ḷ߶ CP4 EPSPS 㳻ⲭ 0.32 10 ᇭ㤳ത࠶ᆀ䟿ḷ䇠 4.5

89 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 മ24. ࣐✝༴⨶30࠶䫏ਾCP4 EPSPSⲴSDS-PAGEỰ⍻ ࣐✝༴⨶ਾⲴ CP4 EPSPS ൘ Tris-⭈≘䞨 4-20%㚊щ✟䞠㜪ࠍ㜦⭥⌣к࠶ ⿫˄3.2 μg ᙫ㳻ⲭ˅ˈ㜦փӞ㬍 G ḃ㢢Ǆ࠶ᆀ䟿অսѪ˄kDa˅Ǆ

⌣䚃 ṧ૱઼༴⨶᧿䘠㳻ⲭ䟿˄μg˅

1 ᇭ㤳ത࠶ᆀ䟿ḷ䇠 4.5 2 CP4 EPSPS 25 ºC ✝༴⨶ 3.2 3 CP4 EPSPS 37 ºC ✝༴⨶ 3.2 4 CP4 EPSPS 55 ºC ✝༴⨶ 3.2 5 CP4 EPSPS 75 ºC ✝༴⨶ 3.2 6 CP4 EPSPS 95 ºC ✝༴⨶ 3.2 7 CP4 EPSPS Ѫ࣐✝༴⨶˄0ºC˅ 3.2 8 ḷ߶ CP4 EPSPS 㳻ⲭ 3.2 9 ḷ߶ CP4 EPSPS 㳻ⲭ 0.32 10 ᇭ㤳ത࠶ᆀ䟿ḷ䇠 4.5

90 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘18. ൘⁑ᤏ㛳⏢˄SGF˅ѝ⎸ॆਾབྷ㛐ᵶ㧼⭏ӗⲴCP4 EPSPS㳻ⲭⲴ∄⍫ᙗ

∄⍫ᙗ ṧ૱઼༴⨶᧿䘠 (䞦⍫অս/mg 㳻ⲭ) ᰐ㛳㳻ⲭ䞦Ⲵᇎ傼ሩ➗ษޫ 0s 4.92 ᰐ㛳㳻ⲭ䞦Ⲵᇎ傼ሩ➗ษޫ 60.min 2.10 བྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ൘ SGF 䟼ษޫ 0s 5.63 བྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ൘ SGF 䟼ษޫ 15 s 0.27 བྷ㛐ᵶ㧼ӗ⭏Ⲵ CP4 EPSPS 㳻ⲭ൘ SGF 䟼ษޫ 30 s 0.15 བྷ㛐ᵶ㧼ӗ⭏Ⲵᴹ CP4 EPSPS 㳻ⲭ൘ SGF 䟼ษޫ 60 s 0.15 ᰐ CP4 EPSPS Ⲵᇎ傼ሩ➗ษޫ 0s 0.02 ᰐ CP4 EPSPS Ⲵᇎ傼ሩ➗ษޫ 60 min 0.05 㕃ߢ⏢オⲭ 0.01

㺘19. ࣐✝༴⨶15࠶䫏ਾCP4 EPSPS䞦⍫࣋

CP4 EPSPS ࣏㜭⍫ᙗ˄U/mg˅ ༴⨶⑙ᓖ ⴨ሩ䞦⍫ 3 ˄ᒣ൷ᮠ 1 ±SD2˅ ᵚ࣐✝˄0ºC˅ 6.03 ± 0.29 100% 25 ºC 4.88 ± 0.24 81% 37 ºC 5.08 ± 0.33 84% 55 ºC 4.22 ± 0.12 70% 75 ºC < LOD4 <3% 95 ºC < LOD4 <3%

˗ᇊѪ࣐✝༴⨶Ⲵ CP4 EPSPS 䞦⍫Ѫ 100%ٷ n=2˗2 SD = ḷ߶ᐞ˗3 1 4 LOD ѪỰ⍻л䲀ˈվҾ LOD Ⲵ CP4 EPSPS 䞦⍫ᇊѹѪк䘠 3 њ✝༴⨶ḷ߶ᐞⲴᒣ൷Ǆ

㺘20. ࣐✝༴⨶30࠶䫏ਾCP4 EPSPS䞦⍫࣋

CP4 EPSPS ࣏㜭⍫ᙗ˄U/mg˅ ༴⨶⑙ᓖ ⴨ሩ䞦⍫ 3 ˄ᒣ൷ᮠ 1 ±SD2˅ ᵚ࣐✝༴⨶˄0ºC˅ 2.8 ± 0.26 100% 25 ºC 3.1 ± 0.23 110% 37 ºC 2.5 ± 0.05 88% 55 ºC 0.70 ± 0.09 25% 75 ºC < LOD4 <8% 95 ºC < LOD4 <8%

˗ᇊѪ࣐✝༴⨶Ⲵ CP4 EPSPS 䞦⍫Ѫ 100%ٷ n=2˗2 SD = ḷ߶ᐞ˗3 1 4 LOD ѪỰ⍻л䲀ˈվҾ LOD Ⲵ CP4 EPSPS 䞦⍫ᇊѹѪк䘠 3 њ✝༴⨶ḷ߶ᐞⲴᒣ൷Ǆ

91 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 7˅CP4 EPSPS 㳻ⲭⲴᙕᙗ㓿ਓ∂ᙗᇎ傼

ᣇ㥹⭈㟖⦹㊣ MON 87427 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭоަᆳᐢ㓿୶ъॆཊᒤ Ⲵᣇ㥹⭈㟖֌⢙ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭᱟаṧⲴˈࡠⴞࡽѪ→ˈᐢ㓿୶ъॆ ᓄ⭘Ⲵ䖜 cp4 epsps สഐᣇ㥹⭈㟖֌⢙ᴹ 6 ⿽ˈवᤜ㤌㬯ǃ⋩㨌ǃ⦹㊣ǃỹ㣡ǃ བྷ䉶઼⭌㨌Ǆ⭡Ҿ㺘䗮Ⲵ㳻ⲭ⴨਼ˈᒦфᐢ䇱ᇎἽ⢙ᶕⓀ CP4 EPSPS 㳻ⲭоབྷ 㛐ᵶ㧼ᶕⓀ㳻ⲭⲴㅹ਼ᙗˈ䘉Ӌ䖜สഐ֌⢙ᆹޘᙗ䇴ԧᡰ⭘Ⲵ㳻ⲭ㓿ਓᙕᙗ∂ᙗ 䈅傼ᣕ੺൷䟷⭘ Hammond Ҿ 1993 ᆼᡀⲴ CP4 EPSPS 㳻ⲭ㓿ਓᙕᙗ∂ᙗ䈅傼Ǆ

㳻ⲭ∂㍐а㡜ᱟ䙊䗷ᙕᙗ֌⭘ᵪࡦӗ⭏∂ᙗˈ᧕䀖ਾࠐሿᰦ㠣ࠐཙ޵ቡՊ㺘 ⧠ࠪн䘲৽ᓄˈа㡜нՊ䎵䗷аઘ˄Sjoblad et al., 1992; Pariza and Johnson, 2001; Hammond and Fuchs, 1998˅Ǆᐢ⸕ⲴἽ⢙㳻ⲭ∂㍐䜭ᱟ൘ᮠሿᰦ޵ӗ⭏∂ᙗˈ∄ ྲ˖㬆哫∂㍐ᱟ 3-24 ሿᰦǃ㓒䉶∂㍐ᱟ 6-8 ሿᰦǃἽ⢙ࠍ䳶㍐ A ѪሿҾ 3 ሿᰦǄ 㘼ᐢ⸕Ⲵ㓶㧼㳻ⲭ∂㍐਼ṧՊ൘ᖸ⸝ᰦ䰤㺘⧠ࠪ∂ᙗ⯷⣦ˈྲ䴽ҡ⯵∂Ѫ 1-5 ཙǃ 㪑㨴⨳㧼㛐∂㍐ B Ѫ 1-6 ሿᰦǃ㚹∂㧼∂㍐Ѫ 18-36 ሿᰦǄ

Hammond˄1993˅࡙⭘བྷ㛐ᵶ㧼㺘䗮㓟ॆ㳻ⲭˈԕ 49ǃ154 ઼ 572 mg/kg bwt ޡ 3 њࡲ䟿ሩ 10 ਚ䳴ᙗ઼ 10 ਚ䳼ᙗ CD-1 ሿ啐䘋㹼 CP4 EPSPS 㳻ⲭ♼㛳༴⨶Ǆ オⲭሩ➗㓴⭘ 50 mM ⻣䞨≒䫐㕃ߢ⏢༴⨶䳴ᙗ઼䳼ᙗሿ啐਴ 10 ਚˈࡲ䟿Ѫ 33.33 ml/kg bwtǄ䱤ᙗሩ➗㓴⭘⢋㹰␵ⲭ㳻ⲭ˄BSA˅༴⨶ 10 ਚ䳴ᙗ઼ 10 ਚ䳼 ڕ઼⦷ᙗ CD-1 ሿ啐ˈ♼㛳ࡲ䟿Ѫ 363 mg/kg bwtǄᇎ傼ᵏ䰤ˈ⇿ཙє⅑䈳ḕ↫ӑ ᓧ⣦ߥǄ༴⨶ㅜ 0 ཙ઼ㅜ 7 ཙ࠶࡛䘋㹼а⅑䈖㓶ⲴѤᒺ㿲ሏᒦ⍻ᇊփ䟽઼䘋伏䟿Ǆ ⭡Ҿ⋑ᴹ㿲ሏࡠн㢟৽ᓄˈㅜ 8 ཙ༴↫ሿ啐ᒦ䘋㹼⯵⨶ᆖ㿲ሏˈ㔃᷌ҏᵚਁ⧠ᔲ ᑨ˄㿱ᵜ⭣䈧Җл޼ᢰᵟᣕ੺ 10˅ǄHammond˄1993˅⹄ウѝ 7 ཙ㿲ሏᵏ⏥ⴆ Ҷབྷཊᐢ⸕㳻ⲭ∂㍐ਁᥕ∂ᙗⲴᴹ᭸ᰦ䰤ˈণ֯䘉ᰦ㓸→䈅傼ˈҏᓄ䈕ਟԕ⺞䇔 CP4 EPSPS 㳻ⲭ൘䈅傼ࡲ䟿лнާᴹ∂ᙗǄഐ↔ˈCP4 EPSPS 㳻ⲭԕᇎ傼ѝⲴ ᴰ儈ࡲ䟿 572 mg/kg bwt ♼㛳ᰦˈ⋑ᴹሩሿ啐ӗ⭏ԫօн࡙ᖡ૽Ǆਟԕ䇔Ѫ CP4 EPSPS Ⲵᰐ᰾ᱮᦏᇣ֌⭘ࡲ䟿˄NOAEL˅㠣ቁѪ 572 mg/kg bwtǄ ൘ Hammond˄1993˅Ⲵ䈅傼ਾˈᆏኡ䜭ޜਨ䘈䪸ሩᣇ㥹⭈㟖⦹㊣ NK 603 ѝⲴ CP4 EPSPS 㳻ⲭ䘋㹼Ҷਖཆа亩㓿ਓᙕᙗ∂ᙗ䈅傼˄Bonnette, 2002˅Ǆ Bonnette˄2002˅䈅傼ѝⲴ CP4 EPSPS 㳻ⲭо Hammond˄1993˅ᡰ⭘㳻ⲭӵ⴨ ᐞањ≘ส䞨ˈ਼Ⓚᙗ䗮ࡠ 99.9%˄㿱к㺘˅ǄBonnette˄2002˅൘䈅傼ѝ䟷⭘ Ⲵ㳻ⲭ♼㛳ࡲ䟿Ѫ 817 mg/kgˈѤᒺ㿲ሏᵏѪ 14 ཙˈ਼ṧ⋑ᴹ㿲ሏࡠ CP4 EPSPS ൘䈅傼ᴰ儈ࡲ䟿≤ᒣлሩሿ啐ާᴹн㢟᭸ᓄˈ䈅傼ᡰᗇ㔃䇪о Hammond˄1993˅ 㔃䇪аṧǄBonnette˄2002˅Ⲵ䈅傼ᣕ੺ᐢ䲿ᣇ㥹⭈㟖⦹㊣ NK 603 Ⲵᆹޘ䇴ԧ ⭣䈧䙂Ӕࡠޘ⨳਴ഭᇦⲴ⌅㿴ⴁ㇑ᵪᶴˈᒦ㻛ᇑḕ઼ᢩ߶Ǆ 2015 ᒤˈ⭡Ҿߌъ䜘䇴ᇑуᇦሩ CP4 EPSPS 㳻ⲭሿ啐ᙕᙗ∂ᙗ⹄ウᵚѕṬ 䚥ᗚߌъ䜘ޣҾᙕᙗ∂ᙗ䇴ԧᯩ⌅ᆈ൘亮㲁ˈᆏኡ䜭ޜਨ޽⅑ሩ CP4 EPSPS 㳻 ⲭ䘋㹼Ҷሿ啐㓿ਓ♼㛳ᙕᙗ∂ᙗ䈅傼ǄSmedley˄2015˅ㅹӪ࡙⭘བྷ㛐ᵶ㧼㺘䗮㓟ॆⲴ CP4 EPSPS 㳻ⲭԕ㓿ਓᯩᔿԕ 2000 mg/kg փ䟽ࡲ䟿অ⅑♼㛳䳼䳴਴ 10 ਚ CD-1 ሿ啐Ǆ਼ᰦ䘋㹼䈅傼Ⲵ䘈ᴹⓦࡲሩ➗㓴ԕ৺ԕ਼ㅹࡲ䟿ሿ⢋㹰␵㳻ⲭ ˄BSA˅♼㛳Ⲵ㳻ⲭሩ➗㓴Ǆᡰᴹࣘ⢙൘♼㛳ࡽ൷⾱伏༴⨶ˈ♼㛳ਾ⇿ཙє⅑㿲 92 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ሏሿ啐ᱟ੖↫ӑᡆᱟ㺘⧠ࠪѝ∂⯷⣦ˈ㿲ሏᵏѪ 14 ཙǄㅜ 14 ཙᰦሶᡰᴹࣘ⢙༴ ↫ˈ䘋㹼བྷփ䀓ࢆǄ⹄ウ㔃᷌㺘᰾ˈᰐ䇪Ӿሿ啐ᆈ⍫⦷ǃѤᒺ㺘⧠ǃփ䟽ǃփ䟽 ໎࣐ǃ伏⢙⎸㙇ᡆབྷփ⯵⨶ᆖᯩ䶒ⴻˈ൷ᵚਁ⧠о༴⨶⴨ޣⲴн㢟৽ᓄǄഐ↔ˈ 䙊䗷䈕ሿ啐ᙕᙗ∂ᙗ⹄ウᗇࡠⲴ CP4 EPSPS 㳻ⲭⲴᰐ᰾ᱮᦏᇣ֌⭘ࡲ䟿 ˄NOAEL˅㠣ቁѪ⹄ウᴰ儈ࡲ䟿 2000 mg/kg bwˈ⭡↔ՠ㇇ᗇࡠ䙊䗷㟣伏ᩴޕ MON 87427 ѝ CP4 EPSPS 㳻ⲭⲴ᳤䵢䲀˄MOE˅儈䗮 2.76 × 106ǄSmedley˄2015˅ Ⲵ䈖㓶䈅傼㔃᷌䈧৲㿱ᵜ⭣䈧Җл޼ᢰᵟᣕ੺ 14Ǆ 2016 ᒤࡍˈᆏኡ䜭ޜਨ޽⅑ሩ CP4 EPSPS 㳻ⲭ䘋㹼Ҷሿ啐㓿ਓ♼㛳ᙕᙗ∂ ᙗ䈅傼˄ᢰᵟᣕ੺ 15˅ǄSmedley˄2016˅ㅹӪ࡙⭘བྷ㛐ᵶ㧼㺘䗮㓟ॆⲴ CP4 EPSPS 㳻ⲭԕ㓿ਓᯩᔿԕ 5000 mg/kg փ䟽ࡲ䟿অᰕє⅑♼㛳䳼䳴਴ 10 ਚ CD-1 ሿ啐ˈ 㿲ሏ 14 ཙǄо Smedley˄2015˅⹄ウаṧˈSmedley˄2016˅䈅傼䇮䇑ѫ㾱׍ᦞ EPA OPPTS 870.1100ˈҏ┑䏣ߌъ䜘亱ਁⲴ⴨ޣḷ߶ NY-2031-16-2013Ǆ⹄ウѝ ൷ᵚਁ⧠ᇎ傼ࣘ⢙ᴹԫօо CP4 EPSPS 㳻ⲭ⴨ޣⲴн㢟৽ᓄǄഐ↔ˈк䘠ཊ⅑ ᓧڕ䘋㹼Ⲵᡰᴹᙕᙗਓᴽ∂ᙗ⹄ウ㔃᷌৽༽䇱ᇎˈCP4 EPSPS 㳻ⲭሩପңࣘ⢙Ⲵ ⋑ᴹԫօн㢟ᖡ૽Ǆ

8˅90 ཙབྷ啐லޫ䈅傼 ਇߌъ䜘ငᢈˈ2014 ᒤߌъ䜘䖜สഐ⭏⢙伏⭘ᆹޘⴁⶓỰ傼⍻䈅ѝᗳ˄ཙ ⍕˅᳘ཙ⍕ᐲ⯮⯵亴䱢᧗ࡦѝᗳሩ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ MON 87427 ৺ަ䶎䖜สഐᑨ㿴⦹㊣ሩ➗ EXP262 䘋㹼Ҷᣇ㩕ޫഐᆀਜ਼䟿Ự⍻઼ 90 ཙབྷ啐ޘ 伏૱லޫ䈅傼Ǆ ᣇ㩕ޫഐᆀਜ਼䟿Ự⍻㔃᷌㺘᰾ˈ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ MON 87427 ѝἽ䞨ਜ਼䟿Ѫ 20.3 mg/gˈ䶎䖜สഐᑨ㿴⦹㊣ EXP262 ѝἽ䞨ਜ਼䟿Ѫ 20.2 mg/gˈ є㘵⋑ᴹᱮ㪇ᐞᔲǄ ሶ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣ MON 87427 ઼䶎䖜สഐӢᵜ⦹㊣ EXP262 侢ᯉ˄∄ֻѪ 50% ǃ25% ǃ12.5%˅ல侢བྷ啐 90 ཙˈல侢ᵏ䰤ࣘ⢙⍫ޕ᧪࠶࡛ ࣘ㠚ྲˈ㻛∋ᴹݹ⌭ˈ啫ǃ⵬ǃਓ㞄ᰐᔲᑨ࠶⋼⢙Ǆ㔏䇑㔃᷌㺘᰾䜘࠶ᑨ㿴ሩ➗ ৺䖜สഐਇ䈅⢙㓴ࣘ⢙䘨伏䟿оᑨ㿴ส⹰侢ᯉሩ➗㓴∄䖳ˈᴹ㔏䇑ᆖ᜿ѹ ˄P<0.05˅ˈն൷ᰐࡲ䟿৽ᓄޣ㌫ˈᵚ㿱ަ⭏⢙ᆖ᜿ѹǄަ։਴㓴ࣘ⢙փ䟽ǃ䘋伏䟿ǃᙫ໎䟽ǃᙫ䘋伏䟿઼ᙫ伏⢙࡙⭘⦷оᑨ㿴ส⹰侢ᯉሩ➗㓴∄䖳ˈᐞᔲ൷ᰐ 㔏䇑ᆖ᜿ѹ˄P>0.05˅Ǆ ᇎ傼ѝᵏ઼ᇎ傼ᵛᵏ਴亩㹰⏢ᆖᤷḷ˄㓒㓶㜎䇑ᮠǃ㹰㓒㳻ⲭǃ㹰ሿᶯǃⲭ 㓶㜎䇑ᮠ৺࠶㊫˅ǃ㹰⭏ॆᤷḷ˄㹰␵щ≘䞨≘ส䖜〫䞦ǃཙ䰘ߜ≘䞨≘ส䖜〫䞦ǃቯ㍐≞ǃ㛶പ䞷ǃ⭈⋩й䞟ǃ㹰㌆ǃң䞨㝡≒䞦ǃᙫ㳻ⲭǃⲭ㳻ⲭǃ㚼䞀ǃ ᙗ⼧䞨䞦˅൷൘ᵜỰ⍻অս↓ᑨ٬㤳ത޵Ǆࣘ⢙䀓ࢆᰦˈབྷփ㿲ሏᵚਁ⧠ᔲᑨǄ⻡䳴ᙗࣘ⢙Ӣѝ㓴㛮/փ∄վҾሩ➗㓴ˈӢ儈㓴㛍/փ∄儈Ҿሩ➗㓴ˈ䖜վ㓴㛍/փ∄ 儈Ҿሩ➗㓴ˈ䖜վ㓴ᗳ/փ∄儈ҾӢվ㓴ˈ䖜ѝ㓴㛮к㞪/փ∄儈ҾӢѝ㓴ˈᐞᔲ ᴹ㔏䇑ᆖ᜿ѹ˄P<0.05˅Ǆ䳼ᙗࣘ⢙Ӣ儈㓴㜨㞪/փ∄ǃ㛮/փ∄儈Ҿሩ➗㓴ˈ䖜 ѝ㓴㛍/փ儈Ҿሩ➗㓴ˈ䖜儈㓴㜨㞪/փ∄ǃ㛮/փ∄儈Ҿሩ➗㓴ˈ䖜ѝ㓴㛍/փ儈Ҿ Ӣѝ㓴ˈᐞᔲᴹ㔏䇑ᆖ᜿ѹ˄P<0.05˅Ǆնк䘠ᐞᔲᰐࡲ䟿৽ᓄޣ㌫ˈᵚ㿱ަ⭏ 93 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ٬оሩ➗㓴∄䖳ˈᐞᔲ൷ᰐ㔏䇑ᆖ∄ᆖ᜿ѹǄަ։਴㓴ࣘ⢙㜿ಘ䟽䟿઼㜿/փ⢙ ᜿ѹ˄P<0.05˅Ǆ⯵⨶㓴㓷ᆖỰḕᵚਁ⧠䈕䖜สഐṧ૱ᕅ䎧ᇎ傼ࣘ⢙ިර⯵⨶㓴㓷ᆖ᭩ਈ઼⢩ᔲᙗᦏՔǄ 㔬кˈ90 ཙབྷ啐ޘ伏૱ல侢䈅傼㺘᰾ˈ䖜 cp4 epsps สഐᣇ䲔㥹ࡲ⦹㊣侢ᯉ˄∄ֻѪ 50% ǃ25% ǃޕ᧪MON 87427 ઼䶎䖜สഐӢᵜ⦹㊣ EXP262 ࠶࡛ 12.5%˅ல侢བྷ啐 90 ཙˈᵚ㿱ަሩࣘ⢙ӗ⭏н㢟ᖡ૽Ǆ

9˅MON 87427 ᶕⓀ CP4 EPSPS 㳻ⲭⲴӪ㗔㟣伏ᩴޕ仾䲙䇴ՠ ṩᦞ CP4 EPSPS 㳻ⲭⲴପңࣘ⢙ᙕᙗ∂ᙗᇎ傼㔃᷌ˈᶕ䇴ՠᲞ䙊Ӫ㗔ഐ㟣伏⎸䍩 MON 87427 ⦹㊣㘼ᩴޕ CP4 EPSPS 㳻ⲭⲴ≤ᒣ઼᭸ᓄˈᱟᆹޘᙗ䇴ԧⲴ ањ䟽㾱ᯩ䶒Ǆ䙊䗷ሿ啐ᙕᙗ∂ᙗᇎ傼ѝᰐ᰾ᱮᦏᇣ֌⭘ࡲ䟿˄NOAEL˅઼㟣 ᓧⲴ▌൘仾䲙Ǆڕ᳤䵢䟿ਟԕ䇑㇇᳤ࠪ䵢䲀˄MOE˅ˈ䘋㘼䇴ՠሩӪ㊫ޕ伏ᩴ ᓧ⣦ߥⴁ⍻—2010-2013 ᒤ㔬ڕ⭡Ҿ 2015 ᒤছ䇑ငᯠਁᐳⲴǉѝഭት≁㩕ޫо ਸᣕ੺Ǌѝ⋑ᴹѝഭӪ㗔ሩ⦹㊣Ⲵ㟣伏ᩴޕ䟿Ⲵᯠᮠᦞˈሩ MON 87427 ᶕⓀ 仾䲙䇴ՠӽ❦֯⭘Ҷ㘏ࠔ㤡઼ᶘᲃݹ˄2006˅൘ޕCP4 EPSPS 㳻ⲭⲴӪ㗔㟣伏ᩴ ߥǊ⣦ޕᓧ⣦ߥᶑḕᣕ੺ѻҼ˄2002˅üü㟣伏о㩕ޫ㍐ᩴڕǉѝഭት≁㩕ޫо ѝࡇࠪࠪѝഭӪᒣ൷⎸䍩⦹㊣Ⲵ䈳ḕᛵߥǄṩᦞ䈕亩䈳ḕˈѝഭӪ⇿ཙ⇿Ӫᒣ൷ Ⲵ⦹㊣⎸䍩䟿Ѫ 9.9 gˈṩᦞѝഭӪᒣ൷փ䟽㓖 50 kgˈᣈ㇇ᡀ⇿ཙ⇿ޜᯔփ䟽⎸ 䍩䟿ࡉѪ 0.2 g˄㺘 21˅Ǆ ൘䈕亩䈳ḕѝˈ⋑ᴹ⎹৺⇿Ӫ⇿ཙᴰབྷⲴ⦹㊣ᶕⓀ伏⢙˄वᤜ⦹㊣઼ਜ਼ᴹ⦹ Ҿᙕᙗ⎸䍩Ⲵՠ䇑ᱟ׍ᦞޣ䟿˄ᙕᙗ⎸䍩˅Ǆഐ↔ˈᵜᣕ੺ѝޕ㊣Ⲵ伏૱˅Ⲵᩴ ц⭼ছ⭏㓴㓷ޘ⨳⧟ຳⴁ᧗㌫㔏 – 伏૱⊑ḃⴁ᧗৺䇴ՠ䇑ࡂ˄GEMS/Foodˈ www.who.int/foodsafety/chem/gems˅Ⲵ䈳ḕᮠᦞǄGEMS/Food 亩ⴞᨀ׋Ⲵᱟ਴ њഭᇦᲞ䙊⎸䍩Ӫ㗔䙊䗷㟣伏⇿ཙᇎ䱵⎸䍩ᴰ儈ᩴޕ䟿Ⲵㅜ 97.5 Ⲯ࠶սⲴ⎸䍩 ⎸⢙㺘 21˅ǄᲞ䙊Ӫ㗔⦹㊣㊹Ⲵᙕᙗ⎸䍩٬ᱟ࠶࡛ṩᦞ⌅ഭ઼◣བྷ࡙ӊⲴ伏˄٬ ⢙䍩䈳ḕᗇࠪⲴǄ㘼⭌⦹㊣઼⠶㊣㣡Ⲵᙕᙗ⎸䍩٬ࡉᱟ࠶࡛ṩᦞ⌠ഭ઼ᰕᵜⲴ伏䍩䈳ḕᗇࠪⲴǄ⭡Ҿ⋑ᴹањᚠᖃⲴᙫⲴㅜ 97.5 Ⲯ࠶սˈҏ⭡Ҿ䘉Ӌ⎸䍩٬⎸ ᶕⓀҾн਼ഭᇦ઼ൠ४ˈഐ↔ᗵ享ሩ䘉Ӌ伏⢙Ⲵᙕᙗ⎸䍩٬࠶࡛ՠ䇑Ǆ ⭡Ҿ⦹㊣Ⲵѫ㾱⭘䙄ᱟ֌Ѫ侢ᯉˈഐ↔ MON 87427 ⦹㊣৺ަ࣐ᐕⲴ伏૱ሶ ᶕਚՊঐѝഭӪ⦹㊣⎸䍩Ⲵаሿ䜘࠶Ǆਖཆˈ䲔ⴤ᧕⎸䍩Ⲵ⠶㊣㣡઼ḀӋᖒᔿⲴ ⭌⦹㊣˄勌伏᷌ょ˅ཆˈབྷ䜭ᮠ⦹㊣䜭ᱟн਼⦹㊣␧ਸ൘а䎧Ⲵˈՠ䇑 MON 87427 ⎸ᇊѝഭӪٷᆸⲴᯩᔿ؍൘␧ਸ⦹㊣ѝⲴⲮ࠶ᮠ䶎ᑨഠ䳮Ǆഐ↔ˈᡁԜਟԕ䟷ਆ 䍩Ⲵ⦹㊣ 100%䜭ᱟ MON 87427 ⦹㊣Ǆ Ӿᆹޘ䇴ԧⲴ䀂ᓖᶕ䈤ˈਟԕ⭘ MON 87427 ㊭㋂ѝ CP4 EPSPS 㳻ⲭⲴᒣ൷ ≤ᒣᶕ䇑㇇⦹㊣㊹ǃ勌伏᷌ょ˄⭌⦹㊣˅઼⠶㊣㣡ѝ CP4 EPSPS 㳻ⲭⲴਜ਼䟿Ǆ ṩᦞ 2008 ᒤ㖾ഭ⭠䰤䈅傼ѝਆṧ⍻ᇊⲴᮠᦞˈMON 87427 ㊭㋂Ⲵ CP4 EPSPS 㳻ⲭᒣ൷ਜ਼䟿Ѫ 3.6 g/g 勌䟽˄㿱 2.6.1 䜘࠶˅ǄᶕⓀҾ MON 87427 ⦹㊣㊹ǃ勌伏᷌ょ˄⭌⦹㊣˅઼⠶㊣㣡Ⲵ CP4 EPSPS 㳻ⲭⲴᙕᙗ㟣伏ᩴޕ䟿䈖㿱㺘 22Ǆ ሿ啐ᙕᙗ♼㛳ᇎ傼㔃᷌㺘᰾ CP4 EPSPS 㳻ⲭⲴᰐ᰾ᱮᦏᇣ֌⭘ࡲ䟿 ˄NOAEL˅Ѫ 572 mg/kg/ཙ˄㿱 3.3.1 䜘࠶˅ǄMOE ቡᱟṩᦞ䘉аࡲ䟿઼ 94 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 CP4 EPSPS 㳻ⲭⲴ㟣伏ᩴޕ䟿ᗇࠪⲴǄ⭡㺘 22 ਟԕⴻࠪˈCP4 EPSPS 㳻ⲭⲴ ٬Ⲵ㤳ത൘ 1.4 × 104 㠣 7.9 × 105 ѻ䰤ˈ⴨ᖃҾањփ䟽Ѫ 50 kg ⲴӪ䴰㾱 MOE ཙ⎸䍩㓖 7900 kg ˄䎵䗷㠚䓛փ䟽 150 ؽ˅Ⲵ MON 87427 ⦹㊣㊭㋂Ǆ䘉Ӌᮠ⇿ ٬⴨ᖃབྷˈӾਖаᯩ䶒䘋а↕䇱᰾൘ѝഭ䙊䗷⎸䍩 MON 87427 㘼᳤䵢Ҿ ᓧ仾䲙Ǆᒦфˈṩᦞ 2016 ᒤᴰᯠⲴᙕᙗ∂⨶ᆖ⹄ウ㔃᷌ڕCP4 EPSPS нՊᑖᶕ ˄ᢰᵟᣕ੺15˅ˈCP4 EPSPS㳻ⲭ㓿ਓ♼㛳ሿ啐Ⲵ NOAEL㠣ቁѪ 5000 mg/kg bwˈ MOE Պᴤ儈Ǆ ⭡↔ՠ㇇ᗇࠪⲴ CP4 EPSPS 㳻ⲭ᳤䵢䲀٬

㺘21. ѝഭӪᒣ൷⦹㊣⎸䍩䟿1

ᙫӪਓ⦹㊣ᩴޕ≤ᒣ 伏⢙ ݻ/Ӫ/ཙ ݻ/ॳݻփ䟽/ཙ 2 ⦹㊣ 9.9 0.20 ˅ᓧ⣦ߥᶑḕᣕ੺ѻҼ˄2002˅Ǌ˄㘏ࠔ㤡, ᶘᲃݹ.2006ڕǉѝഭት≁㩕ޫо 1 ”ṩᦞѝഭӪᒣ൷փ䟽Ѫ 50 kg ሶঅս“ݻ/Ӫ/ཙᦒ㇇Ѫ“ݻ/ॳݻփ䟽/ཙ 2 㺘22. ᶕⓀҾMON 87427Ⲵ伏⢙ѝCP4 EPSPS㳻ⲭⲴ㟣伏ᩴޕ᳤઼䵢䲀˄MOE˅ ⎸䍩⦹㊣ 1 CP4 EPSPS 㳻ⲭᩴޕ 2 ᳤䵢䲀˄MOE˅3 (g/kg/d) (μg/kg/d)

สҾѝഭ䈳ḕᮠᦞ 伏⢙˖⦹⦹㊣ а㡜Ӫ㗔 0.20 0.70 7.9 × 105 ˄ᙫӪਓᒣ൷˅

สҾ GEMS/Food ᮠᦞ 伏⢙˖⦹⦹㊣㊹ а㡜Ӫ㗔 2.04 7.3 7.8 × 104 ˄ㅜ 97.5 Ⲯ࠶ս˅

伏⢙˖⠶⠶㊣㣡 а㡜Ӫ㗔 3.33 12.0 4.8 × 104 ˄ㅜ 97.5 Ⲯ࠶ս˅

伏⢙˖勌勌伏᷌ょ а㡜Ӫ㗔 7.16 25.8 2.2 × 104 ˄ㅜ 97.5 Ⲯ࠶ս˅

˗ṩᦞ㘏ࠔ㤡઼ᶘᲃݹ˄2006˅ԕ৺ GEMS/Food ᮠᦞѝⲴᲞ䙊Ӫ㗔Ⲵ⦹㊣⎸䍩≤ᒣ 1 ᇊ⎸䍩Ⲵ⦹㊣ 100%䜭ᱟ MON 87427 ᒦф MON 87427 ㊭㋂ѝⲴ CP4 EPSPS 㳻ⲭٷ 2 ਜ਼䟿Ѫ 3.6 μg/g 勌䟽˄ppm˅˗ 3 ⭘ሿ啐♼㛳ᇎ傼ᗇࠪⲴ NOAEL˄572 mg/kg bw˅䲔ԕՠ㇇Ⲵ㳻ⲭᩴޕ䟿ᗇࡠ MOE ṩᦞ 2016 ᒤᴰᯠⲴᙕᙗ∂⨶ᆖ⹄ウ㔃᷌ˈCP4 EPSPS 㳻ⲭⲴ NOAEL 㠣ቁѪ 5000˗٬ MOE Պᴤ儈Ǆ mg/kg bwˈ⭡↔ՠ㇇ᗇࠪⲴ CP4 EPSPS 㳻ⲭ᳤䵢䲀٬ 95 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.3.2 䗽ᮅᙝ ṩᦞഭ䱵伏૱⌅ިငઈՊ᧘㦀Ⲵᯠ㳻ⲭ▌൘㠤᭿ᙗ䇴ԧᤷই˄Codex Alimentarius, 2003; Codex Alimentarius, 2009˅ˈḀ⿽㳻ⲭⲴ▌൘㠤᭿ᙗ䴰㾱਼ᰦ 䇴ԧ䈕㳻ⲭⲴ⭏ॆ⢩ᙗԕ৺оᐢ⸕㠤᭿ⓀⲴᒿࡇ⴨լᙗǄྲ᷌䈕㳻ⲭާᴹԕл⢩ ᖱˈࡉа㡜н䇔Ѫ䈕㳻ⲭ኎Ҿ㠤᭿㳻ⲭ˖ i) 䈕㳻ⲭᶕⓀҾ䶎㠤᭿৏˗ ˗ii) 䈕㳻ⲭ൘ᙫ㳻ⲭਜ਼䟿ѝᡰঐ∄ֻᖸሿ iii) 䈕㳻ⲭⲴ≘ส䞨ᒿࡇоᐢ⸕㠤᭿৏Ⲵ≘ส䞨ᒿࡇнާᴹᱮ㪇⴨լᙗ˗ iv) 䈕㳻ⲭ൘ପңࣘ⢙㛳㛐⎸ॆ㌫㔏ѝ䗵䙏㻛⎸ॆ˗ v) 䈕㳻ⲭሩ✝༴⨶нっᇊˈ࣐✝ᶑԦл䗵䙏ཡ⍫Ǆ ሩ MON 87427 ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭⲴ▌൘㠤᭿ᙗ䇴ՠҏᱟ䚥ᗚ䘉Ӌ ৏ࡉˈ䇴ԧ㔃᷌䇔Ѫ CP4 EPSPS 㳻ⲭнާᴹ㠤᭿ᙗǄ 1˅CP4 EPSPS 㳻ⲭⲴ䮯ᵏᆹޘᓄ⭘শਢ ྲࡽ 3.3.1 䜘࠶᧿䘠ˈEPSPS 㳻ⲭᱟ㧭㥹䞨ԓ䉒䙄ᖴѝⲴањ䞦ˈ൘Ἵ⢙઼ ᗞ⭏⢙ѝᲞ䙽ᆈ൘ˈ֌ѪӪ㊫઼ࣘ⢙伏૱ѝⲴᡀ࠶ᐢ㓿ᓄ⭘Ҷ⴨ᖃ䮯ᰦ䰤Ǆ CP4 EPSPS 㳻ⲭᶕⓀҾ൏༔ߌᵶ㧼ˈ኎Ҿ EPSPS 㳻ⲭᇦ᯿Ⲵа⿽Ǆ਴⿽Ἵ⢙઼ ᗞ⭏⢙ѝⲴ EPSPS 㳻ⲭާᴹ儈ᓖⲴ㔃ᶴ⴨լᙗǄ㺘䗮 CP4 EPSPS Ⲵ਴⿽ߌ֌⢙ ˄བྷ䉶ǃ⦹㊣ǃỹ㣡ǃ⋩㨌ǃ㤌㬯઼⭌㨌ㅹ˅ᐢ㓿୶ъॆᓄ⭘䘁 20 ᒤǄ㠣Ӻ⋑ ᴹԫօ、ᆖ䇱ᦞ㺘᰾ CP4 EPSPS 㳻ⲭՊ䙐ᡀӪ㊫䗷᭿Ǆ ᙗޘCP4 EPSPS 㳻ⲭ׋փ⭏⢙Ⲵᆹ˅2 CP4 EPSPS 㳻ⲭⲴ׋փ⭏⢙Ѫ൏༔ߌᵶ㧼 CP4 㧼ṚˈCP4 㧼Ṛਟԕ㺘䗮ཙ ❦Ⲵᒦфሩ㥹⭈㟖н᭿ᝏⲴ EPSPS 㳻ⲭˈᒦભ਽Ѫ CP4 EPSPS 㳻ⲭ˄Padgette et al., 1996˅Ǆ㖾ഭᗞ⭏⢙ษޫᓃ˄American Type Culture Collection˅ᐢ㓿ሩ䘉⿽ CP4 㧼Ṛ䘋㹼Ҷ࠶⿫઼䢤ᇊˈᴰ㓸⺞ᇊ኎Ҿ൏༔ߌᵶ㧼Ⲵ⭏⨶ሿ⿽Ǆⴞࡽ䘈⋑ᴹ ᣕ䚃㺘᰾ԫօа⿽ߌᵶ㧼ሩӪ㊫ᡆ㘵ࣘ⢙ާᴹ㠤⯵㜭࣋ˈ㘼фߌᵶ㧼а㡜нᱟ㠤 ᭿Ⓚ˄FAO/WHO, 1991˅Ǆ↔ཆˈṩᦞ㚄ਸഭ㋞ߌ㓴㓷઼ц⭼ছ⭏㓴㓷˄FAO/WHO, 2001˅Ⲵᣕ੺ˈⴞࡽ䘈⋑ਁ⧠ԫօⲴӪ㗔ሩ㓶㧼ᙗ㳻ⲭ䗷᭿Ǆ 3˅CP4 EPSPS 㳻ⲭⲴ⭏ॆ⢩ᙗ઼㳻ⲭㅹ਼ᙗ࠶᷀

ྲࡽ3.3.1䜘࠶᧿䘠ˈCP4 EPSPSᱟ⭡455њ≘ส䞨㓴ᡀⲴঅ䬮ཊ㛭ˈ⨶䇪࠶ ᆀ䟿Ѫ47.6 kDaǄⴞࡽᐢ⸕ⲴᡰᴹᶕⓀEPSPS䞦˄वᤜCP4 EPSPS˅൘й㓗ᤃᢁ 㔃ᶴк儈ᓖа㠤˄Hammond, et al. 2013˅ǄCP4 EPSPS䞦Ⲵ⤜⢩ᶴ䙐֯ᗇަሩ㥹 ᤱᖸ儈ⲴӢ઼ᙗǄ؍⭈㟖н᭿ᝏˈն਼ᰦሩཙ❦ᓅ⢙PEP EPSPS 䞦˄वᤜ CP4 EPSPS˅ާᴹ儈ᓖⲴᓅ⢙⢩ᔲᙗǄⴞࡽᐢ⸕ሩ EPSPS ਈॆǄ׳䞦ާᴹ⭏⢙ᆖ᜿ѹⲴᓅ⢙ӵѪ S3P ઼ PEPǄ㥹⭈㟖н㜭ਁ⭏ EPSPS 䞦 ٬ǃ⑙ᓖ઼⿫ᆀᕪᓖㅹഐ㍐ᖡ૽Ǆᴰ䘲 CP4 EPSPS 㳻ⲭⲴ䞦⍫ᙗ઼っᇊᙗਇ pH ٬䗷儈ᡆ䗷վ䜭Պ䱽վ䞦⍫ᙗˈpH ሿҾ 5.5 ᰦ CP4 EPSPS 䞦⍫ pH Ѫ 9-9.5ˈpH

96 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䞦⍫ࠐѾᆼޘїཡˈн䗷⭡ pH ᕅ䎧Ⲵ䞦⍫ਈॆᒦ䶎нਟ䘶˄Padgette et al. 1993˅Ǆ CP4 EPSPS ሩ✝нっᇊˈ⑙ᓖ䎵䗷 75°C ᰦˈCP4 EPSPS Ⲵ࣏㜭⍫ᙗণ䱽㠣Ự⍻ 䲀ԕлˈն࣐✝ᒦнᖡ૽㳻ⲭа㓗㔃ᶴⲴᆼᮤᙗǄоᡰᴹިරⲴ㳻ⲭ䞦㊫аṧˈ CP4 EPSPS Ⲵۜॆ⍫ᙗ䘈ਇ৽ᓄⓦ⏢ѝ⿫ᆀᕪᓖⲴᖡ૽Ǆ↓ᑨⲴ䍞ᆈ㕃ߢ⏢˄1 mM KCl˅ᶑԦлˈCP4 EPSPS Ⲵ⢩ᔲ⍫ᙗᴰ儈㜭ཏ䗮ࡠ 5.5 U/mgǄCP4 EPSPS Ⲵ⢩ᔲ⍫ᙗ䲿⵰⿫ᆀᕪᓖ໎བྷ㘼ॷ儈ˈ㠣 50 mM KCl ᰦˈ䞦⍫ᙗ㜭ཏ໎࣐ 2-2.6 ؽ˄Padgette et al. 1993˅Ǆ 䟷⭘а㌫ࡇ࠶᷀ᯩ⌅ሩ MON 87427 ⦹㊣㊭㋂ѝᨀਆ㓟ॆⲴ CP4 EPSPS 㳻ⲭ 䘋㹼Ҷ⢩ᖱ䢤ᇊˈᒦоབྷ㛐ᵶ㧼㺘䗮㌫㔏ѝ㓟ॆᗇࡠⲴ CP4 EPSPS 㳻ⲭ䘋㹼∄ 䖳Ǆ࠶᷀ᯩ⌅वᤜ˖1˅⭘ Bio-Rad 㳻ⲭ࠶᷀㌫㔏⍻ᇊᙫ㳻ⲭ⎃ᓖ˗2˅ॱҼ✧ส ⼪䞨䫐-㚊щ✟䞠㜪ࠍ㜦⭥⌣˄SDS-PAGE˅৺ᇶᓖ䇑䟿˗3˅N-ᵛㄟᒿࡇ࠶᷀˗4˅ ส䍘䖵ࣙ◰ݹ䀓᷀⭥⿫伎㹼ᰦ䰤˄MALDI-TOF˅䍘䉡˗5˅CP4 EPSPS 䞦⍫ᙗỰ ⍻˗6˅㌆สॆ࠶᷀˗7˅䍞ᆈっᇊᙗㅹ࠶᷀ˈᴰ㓸⺞ᇊ MON 87427 Ⲵ CP4 EPSPS 㳻ⲭоབྷ㛐ᵶ㧼⭏ӗⲴ CP4 EPSPS 㳻ⲭާᴹ⴨਼Ⲵ⭏ॆ⢩ᙗˈҼ㘵ᇎ䍘ㅹ਼Ǆ ᴹޣབྷ㛐ᵶ㧼㺘䗮Ⲵ CP4 EPSPS 㳻ⲭᐢᴹབྷ䟿Ⲵ⹄ウᣕ䚃ˈᡰᴹᇎ傼㔃᷌൷㺘 ᰾ᆳᱟᆹޘⲴˈнާᴹ㠤᭿ᙗǄ⭡↔ҏਟԕ᧘ᯝ MON 87427 Ⲵ CP4 EPSPS 㳻ⲭ ҏᱟᆹޘⲴǄ ֻ∄CP4 EPSPS 㳻ⲭঐ㊭㋂ᙫ㳻ⲭⲴ˅4 ൘ MON 87427 ⦹㊣Ⲵ⭏䮯䗷〻ѝˈ䲔⴨ᓄⲴ㣡㊹㓴㓷ཆˈަᆳн਼ಘᇈ㓴㓷޵䜭㜭Ự⍻ࡠ CP4 EPSPS 㳻ⲭ˄㺘 9˅Ǆ൘ᡰᴹ㓴㓷㊫රѝˈ㊭㋂ᱟӪ㊫઼ࣘ ⢙Ⲵ伏⭘䜘սˈഐ↔ᑨ⭘㊭㋂ᶕ䇴ԧަ㠤᭿ᙗǄ൘ MON 87427 ㊭㋂ѝⲴ CP4 EPSPS 㳻ⲭᒣ൷≤ᒣᱟ ˄㺘 9˅ˈ㘼㊭㋂ѝᒣ൷ᙫ㳻ⲭਜ਼䟿Ⲯ࠶ ᮠѪ㊭㋂ᒢ䟽Ⲵ 10.05%˄ᡆ㘵 100500 μg/g dwt˅Ǆ䇑㇇ᗇࡠ CP4 EPSPS 㳻ⲭঐ ㊭㋂ᙫ㳻ⲭⲴ 0.004%˄ @ 100% = 0.004%˅Ǆഐ↔ˈ CP4 EPSPS 㳻ⲭ൘㊭㋂ᙫ㳻ⲭѝⲴ∄ֻᶱվǄ 5˅CP4 EPSPS оަᆳᐢ⸕㠤᭿㳻ⲭⲴ㔃ᶴ⴨լᙗ ṩᦞ CODEX ᤷই˄Codex, 2003˅ˈྲ᷌Ḁ⿽㳻ⲭоᐢ⸕㠤᭿㳻ⲭ൘ԫօ 80 њԕк≘ส䞨⡷⇥޵Ⲵ⴨਼≘ส䞨∄ֻ䗮ࡠ 35%ԕкˈࡉ㺘᰾Ҽ㘵ާᴹ㔃ᶴ⴨լ ᙗˈ䈕㳻ⲭਟ㜭ާᴹ▌൘㠤᭿ᙗǄCODEX ᤷই䘈᧘㦀䟷⭘䘎㔝━㹼デਓỰ㍒〻 ᒿࡔᯝḀ⿽㳻ⲭᱟ੖ާᴹ▌൘㠤᭿ᙗ˄Codex, 2003; Thomas et al., 2005˅Ǆྲ᷌ⴞ Ⲵ㳻ⲭⲴ≘ส䞨ᒿࡇоḀ▌൘㠤᭿ⓀⲴᣇփߣᇊ㈷ᴹ㠣ቁ 8 њ䘎㔝⴨䛫≘ส䞨 ᱟ⴨਼Ⲵˈ䛓Ѹ䈕≘ส䞨ਟԕ㻛䇔Ѫާᴹ▌൘Ⲵ㠤᭿ᙗ˄Metcalfe et al., 1996; Hileman et al., 2002˅Ǆ ࡙⭘ FASTA 䖟Ԧਟԕⴤ᧕∄ሩ≘ส䞨ᒿࡇ˄ণࡍ㓗㓯ᙗᒿࡇ˅ˈ∄ሩⲴ䱥ࡇ 㔃᷌ҏਟԕ⭘Ҿ᧘ᯝᴤ儈㓗Ⲵ㔃ᶴ⴨լᙗ˄ণ㳻ⲭⲴҼ㓗઼й㓗㔃ᶴ˅Ǆ䙊ᑨ㳻 ⲭᒿࡇⲴ⴨լᙗ䎺儈ˈ਼Ⓚᙗҏቡ䎺儈Ǆ㘼਼Ⓚ㳻ⲭᖰᖰާᴹޡ਼ⲴҼ㓗㔃ᶴ઼ й㔤㔃ᶴˈᒦާᴹ⴨਼Ⲵ࣏㜭Ǆ ⭘Ҿ CP4 EPSPS 㳻ⲭ㠤᭿ᙗ࠶᷀ⲴᮠᦞᓃѪ AD_2016ˈवਜ਼ᴹ㠤᭿㳻ⲭǃ

97 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䞷ⓦ㳻ⲭ઼哖䉧㳻ⲭ˄䈖㿱䲿⭣䈧ҖᨀӔⲴᢰᵟᣕ੺ 6˅ǄAD_2016 ᮠᦞᓃवਜ਼ 1,956 ᶑᒿࡇ˄Hileman & Silvanovich, 2016˅ˈᶕⓀҾ伏⢙㠤᭿⹄ウ઼䍴Ⓚ〻ᒿ ٬ᱟỰ㍒ѝሩ㿲ሏࡠ ᮠᦞᓃ FARRP˄2016˅˄http://www.allergenonline.org˅ǄE ٬䎺ሿˈ㺘᰾є⿽㳻ⲭ-Ⲵ⴨լᙗᗇ࠶䲿ᵪਁ⭏ਟ㜭ᙗⲴ㔏䇑ᆖ䟿ᓖǄ∄ሩⲴ E Ⲵᒿࡇ⴨լᙗቡ䎺儈Ǆа㡜㘼䀰ˈᖃєњ㳻ⲭ൘ԫօ 80 њ≘ส䞨⡷⇥޵∄ሩⲴ 䶒ޘ٬䗮ࡠᡆվҾ 1×10-5ˈণਟԕ䇔Ѫ∄ሩⲴє⿽㳻ⲭާᴹ⴨լᙗǄѪҶᴤ-E E=0.01 䘋㹼⭏⢙ؑ᚟ᆖ ᆸⲴ䰸٬؍ൠ䘋㹼䇴ԧˈӾ 2016 ᒤᔰ࿻ˈ䟷⭘ањᶱㄟ ࠶᷀Ǆ൘⴨լᙗ∄ሩѝˈ䘈䟷⭘Ҷ䘎㔝 8 њ≘ส䞨━㹼デਓỰ㍒∄ሩˈԕ⺞ᇊ CP4 EPSPS оᐢ⸕㠤᭿৏൘⸝Ⲵ㓯ᙗཊ㛭кᱟ੖ާᴹ⴨լᙗǄ ⭏⢙ؑ᚟ᆖ∄ሩ㔃᷌㺘᰾ˈCP4 EPSPS 㳻ⲭᒿࡇо AD_2016 ᮠᦞᓃѝⲴ㳻 ٬Ѫ 1.2˄ᢰ-ⲭᒿࡇ䰤⴨լᙗ࠶᷀䇴࠶ᴰվⲴᱟ㕆ਧѪ GI-21725588 Ⲵ㳻ⲭˈަ E ؍٬䗷儈ˈᴰ儈ᗇ࠶઼㿲ሏࡠⲴަԆ∄ሩ㔃᷌н㜭㺘᰾ᆈ൘-ᵟᣕ੺ 6˅Ǆ⭡Ҿ E ᆸᒿࡇᡆ▌൘Ⲵ㠤᭿Ӕ৹⍫ᙗˈ∄ሩᰐᴹ᭸᜿ѹǄഐ↔ᡰᴹ∄ሩ㔃᷌ᵚ䗮ࡠᡆ䎵䗷伏૱⌅ިငઈՊ˄Codex, 2003˅᧘㦀Ⲵ 80 њ≘ส䞨↻ส޵ᴹ 35%≘ส䞨⴨լ ٬ሿҾᡆㅹҾ 1×10-5˅Ǆ↔ཆˈCP4 EPSPS 㳻ⲭᒿࡇо AD_2016-Ⲵ䰸٬ḷ߶˄E ᒿࡇᮠᦞᓃѝ㳻ⲭ䘋㹼∄䖳ᰦˈҏᵚ൘ AD_2016 ᮠᦞᓃѝᩌ㍒ࡠᴹ䘎㔝 8 њ≘ ส䞨⴨਼Ⲵ㳻ⲭǄᙫѻˈ⭏⢙ؑ᚟ᆖ࠶᷀䇱᰾ˈCP4 EPSPS 㳻ⲭоᐢ⸕Ⲵ㠤᭿৏ Ⲵᒿࡇ઼㔃ᶴкⲴ⴨լᙗǄޣнާᴹݽ⯛ᆖ⴨ 6˅CP4 EPSPS 㳻ⲭփཆ⁑ᤏ⎸ॆᇎ傼 Harrison ㅹ˄ 1996˅䇱᰾Ҷ CP4 EPSPS 㳻ⲭ㜭൘⁑ᤏ⎸ॆᶑԦлՊ㻛䗵䙏⎸ ॆᦹǄṩᦞ Western ঠ䘩࠶᷀ˈCP4 EPSPS ൘⁑ᤏ㛳⏢⎸ॆ㌫㔏ѝ 15 s ਾቡᰐ⌅ Ự⍻ࡠǄ ᆏኡ䜭ޜਨሩ CP4 EPSPS 㳻ⲭ൘⿫փ⁑ᤏ㛳⏢˄SGF˅⎸ॆᇎ傼ҏ䇱ᇎҶ к䘠㿲⛩Ǆ䈕䈅傼䟷⭘ഭ䱵⭏ભ、ᆖ⹄ウᡰ˄ILSI˅ޜᐳⲴḷ߶ᯩ⌅˄Thomas et al., 2005˅Ǆ਼ Harrison ㅹ˄1996˅ԕࡽⲴᇎ傼аṧˈᵜ亩⹄ウҏ䟷⭘Ҷབྷ㛐ᵶ㧼 ⭏ӗⲴ CP4 EPSPS 㳻ⲭˈ⎸ॆ⦷䟷⭘ SDS-PAGE ⭥⌣ࠍ㜦ḃ㢢ǃ㳻ⲭ䍘ঠ䘩઼ CP4 EPSPS 䞦৽ᓄ⍫ᙗ䢤ᇊ䘋㹼䇴ՠǄ ᇎ傼㔃᷌䇱ᇎҶ CP4 EPSPS 㳻ⲭ൘փཆ⁑ᤏⲴ SGF 䟼䗵䙏㻛⎸ॆǄ SDS-PAGE ࠍ㜦ḃ㢢㔃᷌㺘᰾ˈ98%ԕкⲴ CP4 EPSPS 㳻ⲭ൘ 15 s ޵㻛䗵䙏⎸ ॆ˄മ 21˅ǄWestern ᵲӔ࠶᷀㔃᷌㺘᰾ˈ95%ԕкⲴ CP4 EPSPS 㳻ⲭ൘ 15 s ޵ ণᰐ⌅Ự⍻˄മ 22˅Ǆ਼ṧˈCP4 EPSPS 㳻ⲭ൘ SGF 䟼ษޫнࡠ 15 sˈަ⍫ᙗ䱽 վࡠ 10%ԕл˄㺘 18˅Ǆᙫѻˈփཆ⁑ᤏ⎸ॆᇎ傼Ⲵ㔃᷌ਟԕᯝᇊ CP4 EPSPS ᓧӗ⭏ড䲙Ǆڕ㳻ⲭ㜭൘⁑ᤏ㛳⏢䟼䗵䙏㻛䱽䀓ˈᡰԕнਟ㜭ሩӪփ 7˅CP4 EPSPS 㳻ⲭⲴ✝っᇊᙗᇎ傼 ࣐✝༴⨶൘⦹㊣㊭㋂ԕ৺ਜ਼ᴹ⦹㊣㊭㋂Ⲵӗ૱࣐ᐕ䗷〻ѝᓄ⭘䶎ᑨᒯ⌋ˈഐ ↔䇴ՠ CP4 EPSPS 㳻ⲭሩ✝༴⨶Ⲵっᇊᙗҏᱟ䶎ᑨ䟽㾱ⲴǄ ✝༴⨶ᇎ傼⭘Ⲵ CP4 EPSPS ᱟ⭡བྷ㛐ᵶ㧼⭏ӗⲴˈ✝༴⨶⑙ᓖѪ 25°Cǃ37°Cǃ 55°Cǃ75°C ઼ 95°Cˈ༴⨶ᰦ䰤Ѫ 15 min ᡆ㘵 30 minǄ䇴ԧⲴ޵ᇩवᤜ˖1˅࣐ 98 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ✝༴⨶ሩ CP4 EPSPS 㳻ⲭ䞦⍫࣋Ⲵᖡ૽˗2˅࣐✝༴⨶ሩ㳻ⲭᆼᮤᙗⲴᖡ૽Ǆ ൘ 25°Cǃ37°Cǃ55°Cǃ75°C ઼ 95°C ⑙ᓖᶑԦл࣐✝༴⨶ 15 ઼ 30 min ਾˈ CP4 EPSPS 㳻ⲭⲴ⍫ᙗ⍻䈅㔃᷌࠶࡛㿱㺘 19 ઼㺘 20Ǆᖃԕ 25ºCǃ37ºC ઼ 55ºC ࣐✝ 15 min ਾ㿲ሏࡠ CP4 EPSPS ⍫ᙗ〽ᴹ䱽վˈ࠶࡛䱽㠣ሩ➗Ⲵ 81%ǃ84%઼ 70%˗ն࣐✝ 30 min ਾˈ25ºC ༴⨶Ⲵ CP4 EPSPS ⍫ᙗ⋑ᴹਈॆˈ37ºC ༴⨶㓴Ⲵ 䞦⍫䱽㠣ሩ➗ⲴⲴ 88%ˈ㘼 55ºC ༴⨶㓴Ⲵ CP4 EPSPS 㺘⧠Ѫབྷᑵл䱽ˈӵѪሩ ➗Ⲵ 25%Ǆн䇪ᱟ༴⨶ 15 min 䘈ᱟ༴⨶ 30 minˈ75°C ઼ 95°C ⑙ᓖᶑԦлⲴ CP4 EPSPS 䞦⍫൷վҾỰ⍻䲀Ǆ SDS-PAGE ࠶᷀㔃᷌㺘᰾ˈ৲➗ḷ߶઼ሩ➗⢙वਜ਼аᶑ㺘㿲࠶ᆀ䟿㓖Ѫ 43.8 kDa ⲴѫᶑᑖˈሩᓄҾ CP4 EPSPS 㳻ⲭ˄മ 23 ઼മ 24˅Ǆ൘ᡰᴹ༴⨶ᰦ䰤 ⌣ˈ༴⨶⑙ᓖᶑԦлˈṧ૱Ⲵ 43.8 kDa ᶑᑖؑਧᕪᓖ⋑ᴹ᰾ᱮⲴ䱽վ˄മ 23઼ 䚃 3~7˗മ 24ˈ⌣䚃 2~7˅Ǆ к䘠㔃᷌㺘᰾ˈ࣐✝༴⨶ӵՊ֯ CP4 EPSPS 㳻ⲭⲴ䞦⍫ᙗ䱽վˈնнՊ֯ CP4 EPSPS 㳻ⲭ䱽䀓Ǆഐ↔ˈнՊᖒᡀᯠⲴ㳻ⲭ⡷⇥㘼ᑖᶕᯠⲴ▌൘㠤᭿仾䲙Ǆ 8˅ਇփⲴ㠤᭿ᙗ࠶᷀ ྲㅜ 1.2.3 䜘࠶ѝᡰ䘠ˈ⦹㊣䮯ᵏ֌ѪӪ㊫઼ࣘ⢙Ⲵ伏⢙ˈ䙊ᑨ኎Ҿ䶎䗷᭿ ৏ᙗ伏⢙Ǆⴞࡽᖸቁᴹޣ伏⭘⦹㊣ӗ૱ਾਁ⭏䗷᭿৽ᓄⲴᣕ䚃˄OECD, 2002˅Ǆ ,.ᣕ䚃˄Pauls and Cross, 1998; Tanaka et alޣ䗷৫ॱࠐᒤѝˈ൘㖾ഭਚᴹєֻ⴨ 2001˅ǄJones et al.˄1995˅䇔Ѫˈ䇨ཊሩ㊭㋂㺘⧠䗷᭿ⲴӪᇎ䱵кᱟሩ㣡㊹䗷 ᭿ˈ䘉ӋӪѝᴹབྷ㓖 80%ⲴӪ䘋㹼伏⢙᭫ࠫ䈅傼ᰦ⋑ᴹӗ⭏Ѥᒺ䗷᭿⯷⣦Ǆ↔ ཆˈ൘ᴮ㓿ሩ㊭㋂ӗ⭏䗷᭿৽ᓄⲴӪѝˈ㲭❦ཊᮠՊሩሿ哖㳻ⲭ䍘ਁ⭏䗷᭿৽ ᓄ˄~75%˅ˈնሩ⦹㊣㊭㋂ӗ⭏䗷᭿৽ᓄⲴӪᶱቁˈаⲮཊս⯵Ӫѝнࡠ 6 ս 䈕ሿݯ、䗷᭿৽ᓄу、ѝᗳᣕ੺ਁ⭏Ҷ䗷᭿৽ᓄǄੁ ᶕ㠚Ҿ᜿བྷ࡙Ⲵєԭᣕ੺˄Pastorello et al., 2000; Pasini et al., 2002˅㺘᰾ˈ ᴹаӋ䗷᭿⯵Ӫࠪ⧠Ⲵ伏૱䗷᭿৽ᓄˈ਼伏⭘वᤜ⦹㊣㋕൘޵Ⲵ⦹㊣ӗ૱ࠪ⧠ Ⲵ䗷᭿৽ᓄ⯷⣦⴨а㠤ˈަѝᶕ㠚᤯⌒䟼Ⲵ 6 ս⯵Ӫ䖫ᓖ伏૱᭫ࠫ䈅傼㔃᷌੸ 䱣ᙗǄնᱟˈк䘠᜿བྷ࡙⹄ウѝᡰᣕ੺Ⲵ⯵Ӫˈ䙊ᑨሩᵲ㥹ǃprunidae ㊫Ἵ⢙ ӗ⭏Ⲵ㣡㊹ǃ㊭㋂৺俉ᯉㅹˈ䜭Պਁ⭏ཊ䟽䗷᭿৽ᓄǄཊ䟽䗷᭿৽ᓄ֯ᗇѤᒺ к䇶࡛⢩ᇊ⯵ֻⲴ㠤᭿৏ᰦާᴹн⺞ᇊᙗ˗⭡Ҿ䗷᭿৽ᓄѻ䰤ᡆཊᡆቁ▌൘Ⲵ Ӕ৹֌⭘ˈ֯ᗇⳞ䈅䇺ᯝ઼⿫փ IgE 㔃ਸ䈅傼ᴤѪ༽ᵲ˄Pastorello et al., 2000˅Ǆ ⦹㊣㊭㋂ѝѫ㾱㠤᭿৏ѻаѪ࠶ᆀ䟿 9kDaⲴ㜲䖜䘀㳻ⲭ˄LTPˈlipid transfer protein˅ˈ኎Ҿ伏⢙ѫ㾱䗷᭿৏ॱޛ䟋ӊᇦ᯿ˈо≤にǃṳᆀоབྷ哖ㅹ䗷᭿৏਼ Ⓚˈ⋑ᴹਁ⧠⦹㊣⢩ᔲⲴ LTP 䗷᭿৏˄Pastorello et al.ˈ2000˅Ǆ⦹㊣оަԆἽ ⢙㣡㊹䗷᭿৏⴨ޣᡀԭሩ∄࠶᷀⋑ᴹਁ⧠⦹㊣⢩ᔲⲴ㠤᭿৏˄Petersen A., 2006˅Ǆⴞࡽˈ䘈⋑ᴹ䏣ཏⲴ䇱ᦞᶕҶ䀓൘᜿བྷ࡙ᡆൠѝ⎧ൠ४⦹㊣ӗ૱䗷᭿৽ ᓄ⍱㹼Ⲵ⢩⛩ˈҏ䘈⋑ᴹᶕ㠚Ҿц⭼ަԆൠ४ޣҾ⦹㊣伏૱䗷᭿৽ᓄⲴᣕ੺ᇎ Ǆ䘉㺘᰾ˈ⭡Ҿ伏⭘⦹㊣৺ަ㹽⭏ӗ૱ˈሩӪ㊫䙐ᡀ䗷᭿৽ᓄ仾䲙Ⲵਟ㜭ᙗֻ ᖸվ˄Scibilia et al., 2008˅Ǆ

99 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.3.3 ᣍ㩛ޱഖᆆ

ṩᦞ OECD˄OECDˈ2002˅Ⲵᣕ䚃ˈ⦹㊣㊭㋂ਜ਼ᴹє⿽ѫ㾱Ⲵᣇ㩕ޫᡀ ࠶ˈণἽ䞨઼ỹᆀ㌆ǄἽ䞨ᆈ൘Ҿ⦹㊣㊭㋂ѝˈ㜭ཏо䫉ǃ䭱ǃ䫮ǃ䫱ਸ䬼ㅹ ⸯ⢙㩕ޫ㷟ਸˈ֯ᗇঅ㛳ࣘ⢙н㜭࡙⭘㊭㋂ѝⲴ䘉Ӌݳ㍐˄Lienerˈ2000˅Ǆ ỹᆀ㌆ᱟᆈ൘Ҿ⦹㊣㊭㋂ѝⲴа⿽࠶ᆀ䟿䖳վфнਟ㻛⎸ॆⲴ⻣≤ॆਸ⢙ˈ⭡ Ҿަӗ≄ਟሬ㠤㛳㛰ˈᡰԕҏ㻛䇔Ѫᱟа⿽ᣇ㩕ޫഐᆀ˄Lienerˈ2000˅Ǆ

Ӿ 2008 ᒤ㖾ഭⲴ⭠䰤䈅傼ѝ 3 њൠ⛩˄⡡㦧ॾᐎⲴ Jefferson 䜑ǃԺ࡙䈪 ԺᐎⲴ Stark 䜑઼䱯㛟㢢ᐎⲴ Jackson 䜑˅ਆṧ䘋㹼ᡀ࠶࠶᷀Ǆ⭠䰤䈅傼䟷⭘ᆼ ޘ䲿ᵪ४㓴䇮䇑ˈ3 ⅑䟽༽Ǆਆṧᶀᯉѝ䘈वᤜ 4 њᐢ㓿୶ъॆ⿽ἽⲴᑨ㿴⦹ ㊣ᵲӔ⿽Ǆਆṧࡽሩ MON 87427 ௧ᯭ㥹⭈㟖˄௧ᯭࡲ䟿Ѫ 1.12 kg ai/ha˅Ǆ

ṩᦞᡰᴹ䈅傼⛩Ⲵᒣ൷㔃᷌˄㺘 23˅ˈ㥹⭈㟖༴⨶Ⲵ MON 87427 оᑨ㿴 ሩ➗ѻ䰤Ἵ䞨Ⲵᒣ൷٬ᆈ൘㔏䇑ᆖᱮ㪇ᙗᐞᔲ˄p<0.05˅ˈնҼ㘵䰤൘ỹᆀ㌆ ਜ਼䟿ᯩ䶒ࡉ⋑ᴹᱮ㪇ᙗᐞᔲǄሩ MON 87427 ઼ሩ➗䰤Ἵ䞨ਜ਼䟿Ⲵᱮ㪇ᐞᔲ䘋㹼䘋а↕Ⲵ࠶᷀ˈਟԕⴻࠪ䘉⿽ᱮ㪇ᙗᐞᔲнާᴹ⭏⢙ᆖ᜿ѹǄ俆ݸˈ㿲ሏࡠ Ⲵᱮ㪇ᙗᐞᔲᖸሿˈMON 87427 ⲴἽ䞨ਜ਼䟿∄ሩ➗վ 5.92%Ǆަ⅑ˈMON 87427 Ἵ䞨ਜ਼䟿Ⲵᒣ൷٬൘୶ъॆ৲➗૱⿽ᒣ൷٬Ⲵ 99%ᇩ䇨४䰤޵Ǆㅜйˈ൘਴њ 䈅傼⛩Ⲵঅ⤜࠶᷀ᰦᒦ⋑ᴹ㿲ሏࡠ MON 87427 ઼ሩ➗䰤ⲴἽ䞨ਜ਼䟿ᴹ㔏䇑ᆖ ᱮ㪇ᙗᐞᔲǄㅜഋˈMON 87427 Ἵ䞨ᒣ൷٬㩭൘ ILSI Ⲵ֌⢙㩕ޫᡀ࠶ᮠᦞᓃ ˄ILSIˈ2006˅ѝ⦹㊣Ἵ䞨ਜ਼䟿㠚❦ਈᔲ㤳ത޵Ǆ ṩᦞ䘉Ӌ㔃᷌ˈӾ伏૱઼侢ᯉᆹޘᡆ㩕ޫ䀂ᓖᶕⴻˈ㥹⭈㟖༴⨶Ⲵ MON 87427 оᑨ㿴ሩ➗ѻ䰤൘ᣇ㩕ޫᡀ࠶ᯩ䶒ᱟᇎ䍘ㅹ਼ⲴǄ 3.3.4 㩛ޱᡆ࠼ MON 87427 Ⲵᡀ࠶࠶᷀㔃᷌ԕ৺оᑨ㿴ሩ➗઼୶ъॆ৲➗૱⿽Ⲵᡀ࠶∄ 䖳㔃᷌㺘᰾ˈMON 87427 ઼ᑨ㿴⦹㊣൘㓴ᡀᡀ࠶кᇎ䍘ㅹ਼Ǆ ᡰᴹ⭘Ҿᡀ࠶࠶᷀Ⲵṧ૱ᶀᯉᱟӾ 2008 ᒤ㖾ഭ⭠䰤䈅傼ѝ䟷䳶ⲴǄ䟷ṧ Ⲵ䈅傼ൠ⛩Ѫ 3 њˈ࠶࡛Ѫ⡡㦧ॾᐎⲴ Jefferson 䜑ǃԺ࡙䈪ԺᐎⲴ Stark 䜑઼ 䱯㛟㢢ᐎⲴ Jackson 䜑Ǆ⭠䰤䈅傼䟷⭘ᆼޘ䲿ᵪ४㓴䇮䇑ˈ࠶࡛䇮 3 ⅑䟽༽Ǆ ਆṧᶀᯉѝ䘈वᤜ਼ᰦ䘋㹼⭠䰤䈅傼Ⲵ 4 њᐢ㓿୶ъॆ⿽ἽⲴᑨ㿴⦹㊣ᵲӔ⿽Ǆ ਆṧࡽሩ MON 87427 ௧ᯭ㥹⭈㟖䘋㹼༴⨶˄⎃ᓖѪ 1.12 kg ai/ha˅Ǆ ࠶᷀Ⲵᡀ࠶वᤜ㩕ޫᡀ࠶ǃᣇ㩕ޫᡀ࠶˄ᐢ൘ 3.3.3 䜘࠶ӻ㓽˅઼⅑⭏ԓ 䉒⢙䍘ǄOECD ޡ䇶᮷Ԧ˄OECD, 2002˅ᐢሩ⦹㊣Ⲵᡀ࠶䘋Ҷ䈖㓶᧿䘠Ǆ㩕ޫ ᡀ࠶वᤜ˖㊭㋂ѝⲴᑨ㿴ᡀ࠶˄⚠࠶ǃᣈ㇇Ⲵ⻣≤ॆਸ⢙ǃ≤࠶ǃ㳻ⲭ䍘઼㜲㛚˅ǃ䞨ᙗ⍇⏔㓔㔤˄ADF˅ǃѝᙗ⍇⏔㓔㔤˄NDF˅ǃᙫ㟣伏㓔㔤˄TDF˅ǃ≘ ส䞨ǃ㜲㛚䞨˄C8~C22˅ǃⸯ⢙䍘˄䫉ǃ䬌ǃ䫱ǃ䭱ǃ䭠ǃ⼧ǃ䫮ǃ䫐઼䬼˅ ઼㔤⭏㍐˄ਦ䞨ǃ✏䞨ǃ㔤⭏㍐ A˄!㜑㩍ঌ㍐˅ǃ㔤⭏㍐ B1ǃ㔤⭏㍐ B2ǃ㔤 ⭏㍐ B6 ઼㔤⭏㍐ E˅ԕ৺ൠк䜘ἽṚⲴᑨ㿴ᡀ࠶ǃADFǃNDFǃ䫉઼⼧Ǆᣇ㩕

100 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ޫᡀ࠶वᤜἽ䞨઼ỹᆀ㌆Ǆ⅑⭏ԓ䉒ӗ⢙वᤜ㌐䟋ǃ䱯兿䞨઼ሩ-俉䉶䞨Ǆഐ↔ˈ ᙫޡ࠶᷀Ҷ 78 ⿽ᡀ࠶˄वਜ਼ᣇ㩕ޫᡀ࠶઼⅑⭏ԓ䉒ӗ⢙˅ˈަѝ㊭㋂ᡀ࠶ 69 ⿽ˈൠк䜘ἽṚᡀ࠶ 9 ⿽Ǆᙫޡ࠶᷀Ⲵ 78 ⿽ᡀ࠶ѝˈᴹ 16 ⿽㊭㋂ᡀ࠶˄15 ⿽ 㩕ޫᡀ࠶઼ 1 ⿽ᣇ㩕ޫᡀ࠶˅䎵䗷 50%Ⲵ࠶᷀⋑ᴹ䗮ࡠᇊ䟿л䲀˄LOQ˅ˈᰐ ⌅䘋㹼㔏䇑࠶᷀Ǆഐ↔ਚሩަᆳᡀ࠶䘋㹼Ҷ㔏䇑࠶᷀Ǆሶᡀ࠶ਜ਼䟿ᣈ㇇Ѫᒢส ਜ਼䟿ਾ䘋㹼㔏䇑࠶᷀ˈਚᴹ≤࠶˄⒯สਜ਼䟿˅઼㜲㛚䞨ਜ਼䟿˄ঐᙫ㜲㛚䞨%˅ ཆǄֻ ⇿⿽ᡀ࠶൷वᤜ 4 њ㔏䇑ሩ∄ˈণ 3 њ䈅傼⛩࠶࡛ሩ∄઼ᡰᴹ䈅傼⛩ᒣ൷ ٬ሩ∄Ǆ㔏䇑ᐞᔲⲴᱮ㪇ᙗ≤ᒣѪ 5%˄`˅Ǆ4 њ୶ъॆ৲➗૱⿽Ⲵᡰᴹ 䈅傼⛩Ⲵᡀ࠶ᒣ൷٬⭘ᶕ⺞ᇊ䈕ᡀ࠶Ⲵ 99%ᇩ䇨४䰤ǄILSI ֌⢙㩕ޫᡀ࠶ᮠᦞ ᓃѝ⦹㊣Ⲵᡀ࠶ਜ਼䟿֌Ѫ⦹㊣ᡀ࠶㠚❦ਈᔲⲴ㤳തǄ MON 87427 ⦹㊣㊭㋂㩕ޫᡀ࠶˖ ൘࠶᷀Ⲵ 69 ⿽㊭㋂ᡀ࠶ѝˈᴹ 64 ⿽Ѫ㩕ޫᡀ࠶ˈवᤜᑨ㿴ᡀ࠶˄5 ⿽˅ǃ ADFǃNDFǃTDFǃ≘ส䞨˄18 ⿽˅ǃ㜲㛚䞨˄22 ⿽˅ǃ㔤⭏㍐˄7 ⿽˅઼ⸯ⢙ ˈ䍘˄9 ⿽˅Ǆ࠶᷀䗷〻ѝǄᴹ 15 ⿽㊭㋂㩕ޫᡀ࠶䎵䗷 50%Ⲵ࠶᷀٬վҾ LOQ ഐ↔⋑ᴹ㓣ޕᴰਾⲴ㔏䇑࠶᷀Ǆ൘䘋㹼㔏䇑࠶᷀Ⲵ 49 ⿽㩕ޫᡀ࠶ѝ˄㺘 24˅ˈ ᴹ 43 ⿽ᡀ࠶Ⲵᒣ൷٬൘ MON 87427 ઼ᑨ㿴ሩ➗䰤нᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲǄ MON 87427 ઼ᑨ㿴ሩ➗ᒣ൷٬ᆈ൘ᱮ㪇ᙗᐞᔲⲴᡀ࠶ᴹ 6 ⿽ˈ࠶࡛Ѫ 16:0 ἅ Ᾰ䞨ǃ18:0 ⺜㜲䞨ǃ18:1 ⋩䞨ǃ18:2 ӊ⋩䞨ǃ20:0 㣡⭏䞨઼ᙫ㜲㛚䞨˄㺘 27˅Ǆ ሩާᴹᱮ㪇ᐞᔲⲴ 6 ⿽㊭㋂㩕ޫᡀ࠶䘋㹼䘋а↕Ⲵ䇴ԧˈਟԕᗇࠪ㔃䇪˖ ൘ާᴹ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴ㩕ޫᡀ࠶ሩ∄ѝˈMON 87427 Ⲵᒣ൷٬о˅1 ᑨ㿴ሩ➗Ⲵᒣ൷٬ᐞᔲᖸሿ˄н䇪ᱟ儈Ҿሩ➗䘈ᱟվҾሩ➗˅ˈণҼ㘵䰤ᐞᔲབྷሿ㤳തӵ⴨ᖃҾሩ➗ᒣ൷٬Ⲵ 1.96%~5.09%˄ާփᶕ䈤ˈ16:0 ἅᾸ䞨Ѫ儈 3.52%ǃ18:0 ⺜㜲䞨儈 3.67%ǃ18:1 ⋩䞨儈 3.22%ǃ18:2 ӊ ⋩䞨վ 1.96%ǃ20:0 㣡⭏䞨儈 4.00%ǃᙫ㜲㛚ਜ਼䟿վ 5.09%˅Ǆ ሩҾ䘉Ӌާᴹ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶ˈMON 87427 Ⲵᒣ൷٬൷൘׍˅2 ᦞ୶ъॆ૱⿽Ⲵᒣ൷٬ᔪ・䎧ᶕⲴ 99%ᇩ䇨४䰤޵˄㺘 24 ઼㺘 27˅Ǆ 䘉ቡ᜿ણ⵰ MON 87427 Ⲵᒣ൷٬༴൘୶ъॆ⦹㊣૱⿽㠚❦ਈᔲ㤳ത ޵Ǆ 3˅ྲ᷌䘋а↕㘳㲁䘉Ӌާᴹ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶൘н਼ൠ⛩Ⲵ㺘⧠ ਟԕⴻࠪˈMON 87427 ઼ሩ➗䰤 18:0 ⺜㜲䞨઼ 20:0 㣡⭏䞨Ⲵᱮ㪇ᙗ ᐞᔲਚ൘ 1 њൠ⛩ࠪ⧠ˈ㘼൘ަᆳ 2 њൠ⛩⋑ᴹࠪ⧠˗16:0 ἅᾸ䞨ǃ 18:1 ⋩䞨઼ 18:2 ӊ⋩䞨Ⲵ㔏䇑ᆖᱮ㪇ᙗᐞᔲࡉ൘ 3 њൠ⛩ࠪ⧠˄㺘 27˅Ǆ 㘼ሩҾᙫ㜲㛚ਜ਼䟿Ⲵᱮ㪇ᙗᐞᔲӵࠪ⧠൘ᡰᴹ䈅傼⛩Ⲵᒣ൷ᮠ∄䖳кˈ ൘ԫօањঅњൠ⛩ˈMON 87427 ઼ሩ➗൷⋑ᴹᱮ㪇ᙗᐞᔲǄնн㇑ ᘾṧˈMON 87427 Ⲵ䘉Ӌᡀ࠶ᒣ൷٬൷൘୶ъॆ૱⿽ᒣ൷٬Ⲵ 99%ᇩ 䇨४䰤޵Ǆ

101 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 4˅ሩҾ䘉Ӌ㺘⧠ࠪ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶ˈн䇪ᱟ MON 87427 䘈ᱟሩ ➗ˈᡀ࠶ᒣ൷٬൷൘᮷⥞ਁ㺘Ⲵ⦹㊣㊭㋂㩕ޫᡀ࠶Ⲵ㤳ത޵ˈҏ൘ ISLI ֌⢙㩕ޫᮠᦞᓃ˄ILSI, 2006˅⴨ޣ⦹㊣㊭㋂㩕ޫᡀ࠶㤳ത޵˄㺘 28˅Ǆ ᙫѻˈ൘䘋㹼㔏䇑࠶᷀Ⲵ 49 ⿽㊭㋂㩕ޫᡀ࠶ѝˈᴹ 43 ⿽ᡀ࠶ MON 87427 ઼ᑨ㿴ሩ➗䰤нᆈ൘㔏䇑ᆖᱮ㪇ᙗᐞᔲˈᴹ 6 ⿽ᡀ࠶ᆈ൘ᱮ㪇ᐞᔲǄ൘䘉Ӌާ ᴹ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶ᯩ䶒ˈMON 87427 ઼ᑨ㿴ሩ➗൘ 16:0 ἅᾸ䞨ǃ18:1 ⋩䞨઼ 18:2 ӊ⋩䞨ᯩ䶒Ⲵᱮ㪇ᐞᔲ൘н਼䈅傼ൠ⛩㺘⧠䖳Ѫа㠤ˈ㘼Ҽ㘵൘ަ ᆳᡀ࠶к˄18:0 ⺜㜲䞨ǃ20:0 㣡⭏䞨઼ᙫ㜲㛚ਜ਼䟿˅ᱮ㪇ᐞᔲ൘н਼ൠ⛩䰤㺘 ⧠на㠤˗նӾᮠ䟿кⴻˈҼ㘵൘ 6 ⿽ᡀ࠶䰤ᆈ൘ᱮ㪇ᙗᐞᔲᖸሿ˗ሩҾᡰᴹ 㺘⧠ࠪᱮ㪇ᙗᐞᔲⲴᡀ࠶ˈMON 87427 Ⲵᒣ൷٬൷㩭൘ᐢਁ㺘᮷⥞઼ ILSI ֌ ⢙㩕ޫᮠᦞᓃⲴ⦹㊣㊭㋂㩕ޫᡀ࠶Ⲵ↓ᑨ㤳ത޵Ǆഐ↔ˈਟԕᗇࠪ MON 87427 ઼ᑨ㿴ሩ➗൘㊭㋂㩕ޫᡀ࠶ᯩ䶒ᇎ䍘ㅹ਼Ⲵ㔃䇪Ǆ MON 87427 ㊭㋂⅑⭏ԓ䉒ӗ⢙˖ ṩᦞ OECD˄2002˅Ǆ⦹㊣㊭㋂ਜ਼ᴹ 3 ⿽ѫ㾱Ⲵ⅑⭏ԓ䉒ӗ⢙˖㌐䟋ǃ䱯兿䞨઼ሩ-俉䉶䞨Ǆ䶎⏰㊹ཊ㚊ᠺ㌆ᱟᱟ㌐䟋Ⲵѫ㾱ᶕⓀ˄Adams et al., 1997˅Ǆ 䱯兿䞨઼ሩ-俉䉶䞨ࡉ⭡㣣俉᯿≘ส䞨˄㤟щ≘䞨઼䞚≘䞨˅ԓ䉒ӗ⭏ˈᱟ㤟ส щ䞨㊫ॆਸ⢙Ⲵࡽփ˄Buchanan et al., 2000˅Ǆ MON 87427 ઼ሩ➗㊭㋂ѝ㌐䟋ⲴỰ⍻൷ሿҾᇊ䟿Ự⍻䲀˄LOQ ˈ ppm˅ˈᵚ䘋㹼㔏䇑࠶᷀ǄMON 87427 Ⲵ䱯兿䞨઼ሩ-俉䉶䞨ᒣ൷٬оᑨ 0.500> ᴹ㔏䇑ᆖᱮ㪇ᙗᐞᔲ˄`˅˄㺘 25˅Ǆ⋐㿴ሩ➗઼୶ъॆ৲➗૱⿽Ⲵᒣ൷٬ ഐ↔ˈӾ⅑⭏ԓ䉒ӗ⢙ᶕⴻˈMON 87427 ઼ᑨ㿴ሩ➗ᇎ䍘ㅹ਼Ǆ MON 87427 ൠк䜘ᮤṚ㩕ޫᡀ࠶˖ ޡ࠶᷀Ҷ MON 87427 ൠк䜘ᮤṚṧ૱Ⲵ 9 ⿽㩕ޫᡀ࠶˄㳻ⲭ䍘ǃ≤࠶ǃ 㜲㛚ǃ⚠࠶ǃ⻣≤ॆਸ⢙ǃADFǃNDFǃ䫉઼⼧˅Ǆṩᦞᡰᴹ䈅傼ൠ⛩Ⲵᒣ൷ 㓿㥹⭈㟖༴⨶Ⲵ MON 87427 оᑨ㿴ሩ➗ѻ䰤Ⲵ਴⿽㩕ޫᡀ࠶⋑ᴹ㔏䇑ᆖˈ٬ ᱮ㪇ᐞᔲ˄p>0.05˅˄㺘 26˅Ǆնྲ᷌࠶ᔰ䈅傼ൠ⛩䘋㹼࠶᷀ˈMON 87427 ઼ሩ➗൘ 1 њ䈅傼ൠ⛩˄⡡㦧ॾᐎ Jefferson 䜑˅Ⲵњ࡛㩕ޫᡀ࠶˄⻣≤ॆਸ⢙ǃ ࠶઼㳻ⲭ䍘˅кᆈ൘㔏䇑ᆖᱮ㪇ᐞᔲ˄㺘 27˅Ǆণ֯㺘⧠ᱮ㪇ᐞᔲˈMON 87427≥ 䘉 3 ⿽ᡀ࠶Ⲵᒣ൷٬൷༴Ҿ୶ъॆ৲➗૱⿽ᒣ൷٬Ⲵ 99%ᇩ䇨४䰤޵ˈҏ൘ ILSI ֌⢙㩕ޫᮠᦞᓃ઼᮷⥞ᣕ䚃Ⲵᮠᦞ㤳ത޵˄㺘 28˅Ǆഐ↔ˈӾ伏૱઼侢ᯉ ᆹޘᡆ㩕ޫ䀂ᓖᶕⴻˈ䘉Ӌᐞᔲ⋑ᴹ⭏⢙ᆖ᜿ѹǄ

102 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘23. MON 87427ԕ৺ᑨ㿴ሩ➗Ⲵᣇ㩕ޫᡀ࠶Ⲵ≷ᙫᒣ൷٬

૱׍ᦞ୶ъॆ ˅ሩ➗ᒣ൷٬ – ᐞᔲ˄MON 87427 ᒣ൷٬ MON 87427 ᑨ㿴ሩ➗ ⿽ᒣ൷٬ᗇࠪ ˅.S.E˄S.E.˅ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅ ᒣ൷٬ *S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ Ⲵᇩ䇨४䰤˄ᒣ൷٬ ˄㤳ത˅ ˄㤳ത˅ ˅㤳ത˄ ˅٬-㤳ത˅ л䲀ˈк䲀 ˄p˄ ᣇ㩕ޫᡀ࠶˄ᒢ䟽%˅ Ἵ䞨 0.96 (0.031) 1.02 (0.031) -0.060 (0.022) -0.10, -0.016 0.008 0.73, 1.23 (0.87 - 1.04) (0.94 - 1.12) (-0.12 - 0.032) (0.82 - 1.07)

ỹᆀ㌆ 0.14 (0.028) 0.15 (0.029) -0.0054 (0.0082) -0.024, 0.013 0.524 0.024, 0.29 (0.098 - 0.21) (0.11 - 0.21) (-0.028 - 0.025) (0.092 - 0.21)

୶ъॆ৲➗૱⿽ 95%㖞ؑ䲀лⲴ 99%ᇩ䇨४䰤ˈྲ᷌Ѫ䍏ᮠࡉ䇮Ѫ 0Ǆ *

103 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᙫ≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬

୶ъॆ৲➗૱⿽ ˅ᑨ㿴ሩ➗ᒣ൷٬ – MON 87427² ᑨ㿴ሩ➗ 4 ᐞᔲ˄MON 87427 ᒣ൷٬ S.E.˅ 95%㖞ؑ४䰤 ᐞᔲᱮ㪇ᙗ ᇩ䇨४䰤 5˄S.E.˅ ᒣ൷٬˄S.E.˅³ ᒣ൷٬˄࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷٬ ˅㤳ത˄ ˅٬-㤳ത˅ ˄㤳ത˅ ˄㤳ത˅ л䲀, к䲀 ˄p˄

ᑨ㿴ᡀ࠶˄ᒢส%˅ ⚠࠶ 1.58 (0.036) 1.56 (0.038) 0.013 (0.042) -0.074, 0.099 0.765 1.13, 1.97 (1.43 - 1.81) (1.48 - 1.67) (-0.14 - 0.14) (1.18 - 1.82)

⻣≤ॆਸ⢙ 84.88 (0.56) 84.51 (0.57) 0.37 (0.33) -0.40, 1.14 0.305 80.77, 89.46 (83.60 - 86.33) (82.96 - 85.76) (-0.87 - 1.63) (82.26 - 87.17)

≤࠶˄⒯ส%˅ 11.62 (0.46) 11.41 (0.46) 0.22 (0.21) -0.27, 0.71 0.337 7.56, 14.80 (10.90 - 13.30) (10.20 - 12.40) (-0.30 - 1.10) (9.31 - 12.70)

㳻ⲭ䍘 10.05 (0.63) 10.26 (0.63) -0.21 (0.38) -1.08, 0.66 0.594 5.79, 13.43 (8.46 - 11.35) (8.62 - 11.92) (-1.50 - 1.20) (8.07 - 12.13)

ᙫ㜲㛚䞨 3.50 (0.13) 3.69 (0.13) -0.19 (0.075) -0.36, -0.015 0.036 2.12, 5.35 (3.13 - 3.83) (3.47 - 3.98) (-0.52 - 0.11) (2.90 - 4.30)

㓔㔤˄ᒢส%˅ 䞨ᙗ⍇⏔㓔㔤˄ADF˅ 3.37 (0.23) 3.19 (0.23) 0.18 (0.27) -0.43, 0.79 0.521 1.84, 4.39 (2.67 - 4.27) (2.80 - 3.54) (-0.27 - 1.09) (2.29 - 4.27)

104 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬

㓔㔤˄ᒢส%˅ ѝᙗ⍇⏔㓔㔤˄NDF˅ 10.00 (0.51) 10.12 (0.51) -0.12 (0.24) -0.68, 0.43 0.628 5.69, 11.81 (9.17 - 10.97) (9.21 - 11.27) (-0.90 - 0.98) (7.06 - 10.66)

ᙫ㟣伏㓔㔤˄TDF˅ 13.00 (0.37) 13.05 (0.37) -0.044 (0.24) -0.53, 0.44 0.854 8.67, 15.32 (12.13 - 14.35) (12.64 - 13.75) (-0.67 - 1.07) (10.25 - 14.30)

≘ส䞨˄ᒢส%˅ щ≘䞨 0.75 (0.061) 0.76 (0.061) -0.0061 (0.033) -0.082, 0.069 0.857 0.32, 1.12 (0.61 - 0.89) (0.55 - 0.90) (-0.15 - 0.080) (0.58 - 0.98)

㋮≘䞨 0.48 (0.024) 0.49 (0.025) -0.010 (0.015) -0.040, 0.020 0.501 0.24, 0.68 (0.40 - 0.55) (0.39 - 0.56) (-0.079 - 0.065) (0.34 - 0.57)

ཙߜ≘䞨 0.64 (0.041) 0.64 (0.042) -0.0025 (0.025) -0.059, 0.054 0.920 0.34, 0.92 (0.54 - 0.71) (0.48 - 0.73) (-0.099 - 0.064) (0.52 - 0.78)

㜡≘䞨 0.24 (0.010) 0.24 (0.010) -0.0022 (0.0068) -0.018, 0.013 0.750 0.14, 0.30 (0.21 - 0.27) (0.21 - 0.26) (-0.015 - 0.020) (0.18 - 0.26)

105 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬

≘ส䞨˄ᒢส%˅ 䉧≘䞨 1.87 (0.15) 1.89 (0.15) -0.020 (0.077) -0.20, 0.16 0.801 0.77, 2.84 (1.53 - 2.24) (1.38 - 2.28) (-0.35 - 0.20) (1.46 - 2.49)

⭈≘䞨 0.38 (0.018) 0.38 (0.018) 0.0012 (0.0098) -0.021, 0.024 0.906 0.23, 0.52 (0.34 - 0.43) (0.31 - 0.42) (-0.038 - 0.033) (0.32 - 0.43)

㓴≘䞨 0.30 (0.013) 0.30 (0.013) -0.0014 (0.0081) -0.018, 0.015 0.867 0.16, 0.39 (0.27 - 0.34) (0.23 - 0.34) (-0.045 - 0.033) (0.22 - 0.33)

ᔲӞ≘䞨 0.35 (0.026) 0.36 (0.027) -0.0018 (0.014) -0.035, 0.032 0.901 0.16, 0.53 (0.29 - 0.42) (0.26 - 0.42) (-0.081 - 0.039) (0.27 - 0.46)

Ӟ≘䞨 1.23 (0.11) 1.25 (0.11) -0.022 (0.060) -0.16, 0.12 0.725 0.43, 1.95 (0.97 - 1.52) (0.89 - 1.56) (-0.29 - 0.13) (0.93 - 1.69)

䎆≘䞨 0.30 (0.012) 0.30 (0.013) -0.0020 (0.0072) -0.018, 0.014 0.782 0.19, 0.40 (0.27 - 0.33) (0.25 - 0.33) (-0.024 - 0.026) (0.26 - 0.34)

106 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬

≘ส䞨˄ᒢส%˅ 㳻≘䞨 0.24 (0.019) 0.24 (0.019) 0.00043 (0.0094) -0.021, 0.022 0.964 0.11, 0.29 (0.20 - 0.29) (0.20 - 0.27) (-0.015 - 0.024) (0.17 - 0.25)

㤟щ≘䞨 0.51 (0.040) 0.52 (0.040) -0.0088 (0.023) -0.063, 0.045 0.714 0.23, 0.75 (0.40 - 0.60) (0.38 - 0.61) (-0.10 - 0.052) (0.39 - 0.66)

㝟≘䞨 0.90 (0.067) 0.90 (0.067) -0.0045 (0.032) -0.078, 0.069 0.889 0.40, 1.24 (0.74 - 1.08) (0.65 - 1.06) (-0.15 - 0.12) (0.66 - 1.07)

э≘䞨 0.47 (0.033) 0.48 (0.033) -0.011 (0.022) -0.062, 0.040 0.625 0.24, 0.66 (0.38 - 0.52) (0.36 - 0.58) (-0.063 - 0.052) (0.38 - 0.59)

㣿≘䞨 0.35 (0.020) 0.35 (0.020) -0.0022 (0.013) -0.032, 0.028 0.871 0.20, 0.46 (0.29 - 0.39) (0.28 - 0.39) (-0.042 - 0.033) (0.28 - 0.41)

㢢≘䞨 0.054 (0.0032) 0.053 (0.0033) 0.00070 (0.0032) -0.0067, 0.0081 0.835 0.032, 0.069 (0.045 - 0.064) (0.042 - 0.065) (-0.015 - 0.013) (0.039 - 0.063)

107 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬

≘ส䞨˄ᒢส%˅ 䞚≘䞨 0.29 (0.029) 0.30 (0.029) -0.0041 (0.026) -0.057, 0.048 0.874 0.077, 0.45 (0.18 - 0.38) (0.21 - 0.39) (-0.12 - 0.11) (0.11 - 0.43)

㕜≘䞨 0.48 (0.029) 0.49 (0.029) -0.0015 (0.017) -0.040, 0.037 0.930 0.25, 0.67 (0.41 - 0.55) (0.37 - 0.56) (-0.089 - 0.049) (0.38 - 0.58)

㜲㛚䞨˄ঐᙫ㜲㛚䞨%˅ 16:0 ἅᾸ䞨 10.91 (0.26) 10.54 (0.26) 0.37 (0.065) 0.22, 0.52 <0.001 6.42, 15.23 (10.44 - 11.52) (10.15 - 11.08) (0.14 - 0.59) (9.13 - 12.33)

18:0 ⺜㜲䞨 1.97 (0.091) 1.90 (0.091) 0.070 (0.028) 0.0048, 0.13 0.038 0.87, 2.88 (1.81 - 2.17) (1.77 - 2.07) (-0.028 - 0.18) (1.54 - 2.38)

18:1 ⋩䞨 c 24.28 (0.92) 23.52 (0.92) 0.76 (0.23) 0.23, 1.28 0.010 11.30, 43.27 (22.84 - 26.62) (21.74 - 25.71) (0.13 - 1.20) (21.39 - 34.71)

18:2 ӊ⋩䞨 60.84 (1.28) 62.06 (1.28) -1.22 (0.29) -1.88, -0.55 0.002 41.35, 74.78 (57.61 - 62.70) (59.18 - 64.09) (-1.69 - -0.46) (49.38 - 63.16)

108 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬

㜲㛚䞨˄ঐᙫ㜲㛚䞨%˅ 18:3 ӊ哫⋩䞨 1.20 (0.014) 1.20 (0.014) -0.0012 (0.015) -0.035, 0.033 0.935 0.78, 1.52 (1.13 - 1.26) (1.18 - 1.22) (-0.088 - 0.043) (0.97 - 1.35)

20:0 㣡⭏䞨 0.42 (0.030) 0.41 (0.030) 0.016 (0.0043) 0.0063, 0.026 0.005 0.15, 0.67 (0.37 - 0.48) (0.37 - 0.46) (-0.0022 - 0.034) (0.32 - 0.53)

20:1 Ҽॱ⻣✟䞨 0.21 (0.0080) 0.21 (0.0080) -0.00097 (0.0017) -0.0049, 0.0029 0.583 0.12, 0.36 (0.19 - 0.23) (0.20 - 0.23) (-0.0049 - 0.0033) (0.21 - 0.31)

22:0 ኡ㩞䞨 0.17 (0.018) 0.16 (0.018) 0.0076 (0.0050) -0.0039, 0.019 0.167 0, 0.32 (0.14 - 0.23) (0.14 - 0.20) (-0.0099 - 0.031) (0.057 - 0.23)

ⸯ⢙䍘䫉˄ᒢส%˅ 0.0060 (0.00063) 0.0055 (0.00063) 0.00049 (0.00033) -0.00027, 0.0013 0.176 0.0019, 0.0076 (0.0048 - 0.0079) (0.0046 - 0.0076) (-0.00037 - 0.0017) (0.0038 - 0.0068)

䬌˄mg/kg dwt˅ 1.63 (0.11) 1.71 (0.12) -0.085 (0.11) -0.33, 0.16 0.458 0.17, 3.48 (1.21 - 2.07) (1.49 - 1.99) (-0.42 - 0.18) (1.10 - 2.62)

109 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬

ⸯ⢙䍘䫱˄mg/kg dwt˅ 23.61 (0.78) 23.03 (0.79) 0.58 (0.61) -0.82, 1.98 0.368 11.42, 28.01 (22.21 - 25.84) (20.66 - 25.57) (-2.12 - 2.11) (16.55 - 24.10)

䭱˄ᒢส%˅ 0.13 (0.0033) 0.13 (0.0033) -0.00021 (0.0034) -0.0080, 0.0076 0.952 0.080, 0.16 (0.13 - 0.14) (0.12 - 0.14) (-0.0062 - 0.010) (0.11 - 0.15)

䭠˄mg/kg dwt˅ 7.91 (1.06) 8.07 (1.06) -0.16 (0.27) -0.71, 0.39 0.567 0, 12.67 (5.52 - 9.40) (4.89 - 9.82) (-0.83 - 0.83) (4.00 - 9.17)

⼧˄ᒢส%˅ 0.34 (0.0034) 0.34 (0.0036) -0.0071 (0.0050) -0.018, 0.0040 0.185 0.24, 0.42 (0.32 - 0.35) (0.33 - 0.35) (-0.020 - 0.0053) (0.28 - 0.37)

䫮˄ᒢส%˅ 0.40 (0.0074) 0.40 (0.0077) -0.0045 (0.0073) -0.019, 0.010 0.546 0.24, 0.54 (0.38 - 0.42) (0.38 - 0.43) (-0.029 - 0.021) (0.33 - 0.46)

䬼˄mg/kg dwt˅ 22.67 (1.06) 23.99 (1.07) -1.32 (1.00) -3.62, 0.99 0.225 11.46, 30.37 (20.99 - 25.42) (21.65 - 28.08) (-5.63 - 3.29) (17.30 - 25.45)

110 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬

㔤⭏㍐˄mg/kg dwt˅ ਦ䞨 0.36 (0.025) 0.39 (0.025) -0.030 (0.030) -0.099, 0.040 0.347 0.11, 0.61 (0.28 - 0.43) (0.29 - 0.49) (-0.097 - 0.078) (0.24 - 0.57)

✏䞨 27.22 (2.15) 27.71 (2.18) -0.48 (1.34) -3.22, 2.26 0.722 7.89, 49.83 (22.56 - 33.37) (22.61 - 33.26) (-3.30 - 2.66) (20.63 - 43.08)

㔤⭏㍐ A 1.01 (0.050) 0.96 (0.051) 0.057 (0.043) -0.029, 0.14 0.186 0.38, 1.68 (0.88 - 1.21) (0.76 - 1.16) (-0.094 - 0.21) (0.58 - 1.50)

㔤⭏㍐ B1 2.97 (0.19) 2.88 (0.20) 0.084 (0.16) -0.28, 0.45 0.606 2.21, 3.65 (2.58 - 3.41) (2.48 - 3.41) (-0.44 - 0.45) (2.41 - 3.48)

㔤⭏㍐ B2 2.09 (0.37) 1.93 (0.37) 0.16 (0.33) -0.59, 0.92 0.630 0, 4.47 (1.17 - 3.56) (1.32 - 2.58) (-0.72 - 1.23) (1.28 - 3.29)

㔤⭏㍐ B6 7.48 (0.60) 7.71 (0.60) -0.23 (0.41) -1.16, 0.70 0.589 2.57, 12.07 (5.91 - 8.69) (5.67 - 9.61) (-1.40 - 1.76) (5.24 - 10.29)

111 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᙫ˄㔝≶㺘24. MON 87427઼ᑨ㿴ሩ➗㊭㋂㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬

㔤⭏㍐˄mg/kg dwt˅ 㔤⭏㍐ E 13.14 (2.09) 13.46 (2.10) -0.31 (0.86) -2.05, 1.43 0.718 0, 25.61 (7.04 - 17.44) (10.13 - 18.10) (-6.54 - 4.52) (6.67 - 17.34)

¹dwt=ᒢ䟽Ǆ ² MON 87427 ௧㥹⭈㟖༴⨶Ǆ S.E.) = ḷ߶ᐞǄ)ˈᒣ൷٬Ѫᴰሿᒣᯩᒣ൷٬ ³ 4 ᑨ㿴ሩ➗о MON 87427 Ѫ䘁ㅹสഐޣ㌫ˈ௧㥹⭈㟖༴⨶Ǆ ୶ъॆ૱⿽Ⲵᒣ൷٬൘ 95%㖞ؑ䲀лⲴ 99%ᇩ䇨४䰤ˈྲ᷌ᱟ䍏ᮠቡ䇮ᇊѪ 0Ǆ 5

112 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᙫ≶㺘 25. MON 87427 ઼ᑨ㿴ሩ➗㊭㋂⅑⭏ԓ䉒ӗ⢙Ⲵᡰᴹ䈅傼⛩ᒣ൷٬

⅑⭏ԓ䉒ӗ⢙˄μg/g dwt˅

䱯兿䞨 2348.63 (58.17) 2387.92 (60.24) -39.29 (81.45) -221.69, 143.10 0.640 1070.41, 2955.86 (2188.55 - 2559.19) (2236.10 - 2500.00) (-171.29 - 209.93) (1588.35 - 2630.98)

ሩ-俉䉶䞨 204.94 (17.45) 205.00 (17.54) -0.060 (8.82) -20.17, 20.05 0.994 58.74, 313.97 (166.11 - 260.43) (162.58 - 252.26) (-28.53 - 32.92) (124.16 - 250.30)

113 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᙫ≶㺘26. MON 87427઼ᑨ㿴ሩ➗ൠк䜘ᮤṚ㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬

ᑨ㿴ᡀ࠶˄ᒢส%˅

⚠࠶ 4.73 (0.23) 4.86 (0.23) -0.13 (0.19) -0.53, 0.27 0.508 2.66, 6.48 (4.39 - 5.13) (3.99 - 5.84) (-0.74 - 0.66) (3.70 - 5.95)

⻣≤ॆਸ⢙ 87.23 (0.90) 86.69 (0.91) 0.54 (0.49) -0.46, 1.54 0.277 80.13, 94.05 (86.21 - 89.23) (83.80 - 88.92) (-1.59 - 2.61) (83.23 - 90.37)

≤࠶˄⒯ส%˅ 68.71 (2.30) 69.76 (2.32) -1.05 (1.06) -3.50, 1.40 0.350 51.70, 86.22 (62.70 - 73.10) (64.10 - 75.00) (-5.90 - 5.70) (61.00 - 76.00)

㳻ⲭ䍘 6.44 (0.75) 6.78 (0.76) -0.34 (0.39) -1.25, 0.57 0.413 1.34, 11.57 (4.48 - 7.40) (5.17 - 8.94) (-2.00 - 1.26) (4.37 - 9.31)

ᙫ㜲㛚 1.60 (0.17) 1.69 (0.18) -0.092 (0.25) -0.65, 0.46 0.720 0.44, 3.33 (1.09 - 1.85) (0.58 - 2.28) (-1.11 - 1.18) (0.78 - 3.16)

㓔㔤˄ᒢส%˅ ADF 24.96 (0.97) 26.74 (1.03) -1.78 (1.42) -4.65, 1.09 0.216 14.84, 38.51 (21.08 - 29.00) (20.27 - 32.16) (-8.15 - 3.58) (21.33 - 35.92)

114 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᙫ≶㺘 26. MON 87427 ઼ᑨ㿴ሩ➗ൠк䜘ᮤṚ㩕ޫᡀ࠶Ⲵᡰᴹ䈅傼⛩ᒣ൷٬

㓔㔤˄ᒢส%˅ 39.79 (1.32) 38.12 (1.38) 1.67 (1.76) -2.32, 5.65 0.368 25.12, 54.99 NDF (36.14 - 43.70) (33.07 - 43.43) (-1.55 - 4.79) (29.68 - 60.16)

ⸯ⢙䍘˄ᒢส%˅ 0.19 (0.010) 0.19 (0.011) -0.0083 (0.011) -0.031, 0.014 0.455 0.075, 0.29 䫉 (0.14 - 0.22) (0.15 - 0.25) (-0.063 - 0.036) (0.10 - 0.24)

⼧ 0.24 (0.021) 0.24 (0.021) -0.0050 (0.013) -0.032, 0.022 0.708 0.063, 0.37 (0.20 - 0.31) (0.19 - 0.31) (-0.074 - 0.038) (0.16 - 0.31)

115 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘27. MON 87427઼ᑨ㿴ሩ➗ᒣ൷٬㺘⧠ࠪ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶≷ᙫ

୶ъॆ৲➗૱⿽ ˅ሩ➗٬ – ٬ ᒣ൷ᐞ˄MON 87427 MON 87427² ᑨ㿴ሩ➗ 4 MON 87427 ࠶᷀Ⲵᡀ࠶˄অս˅¹ ᒣ൷ᐞ 95%㖞ؑ४䰤 ᇩ䇨४䰤 5 ˅㤳ത˄ 3 ᒣ൷٬ ᒣ൷٬ ˄⴨ᖃҾሩ➗%˅ л䲀, к䲀 ˄㤳ത˅

ᡰᴹ䈅傼⛩ᒣ൷٬ᆈ൘ᱮ㪇ᙗᐞᔲ

ᑨ㿴ᡀ࠶˄ᒢส%˅

ᙫ㜲㛚 3.50 3.69 -5.09 0.036 3.13 - 3.83 2.12, 5.35

㊭㋂㜲㛚䞨˄ঐᙫ㜲㛚䞨%˅

16:0 ἅᾸ䞨 10.91 10.54 3.52 <0.001 10.44 - 11.52 6.42, 15.23

18:0 ⺜㜲䞨 1.97 1.90 3.67 0.038 1.81 - 2.17 0.87, 2.88

18:1 ⋩䞨 24.28 23.52 3.22 0.010 22.84 - 26.62 11.30, 43.27

18:2 ӊ⋩䞨 60.84 62.06 -1.96 0.002 57.61 - 62.70 41.35, 74.78

20:0 㣡⭏䞨 0.42 0.41 4.00 0.005 0.37 - 0.48 0.15, 0.67

㊭㋂ᣇ㩕ޫᡀ࠶˄ᒢส%˅

Ἵ䞨 0.96 1.02 -5.92 0.008 0.87 - 1.04 0.73, 1.23

116 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅㺘 27. MON 87427 ઼ᑨ㿴ሩ➗ᒣ൷٬㺘⧠ࠪ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶≷ᙫ˄㔝

ањԕк䈅傼⛩ᮠ٬ᆈ൘ᱮ㪇ᙗᐞᔲ

㊭㋂㜲㛚䞨˄ঐᙫ㜲㛚䞨%˅

16:0 ἅᾸ䞨 - ൠ⛩˖ARNE 11.49 10.99 4.53 <0.001 11.47 - 11.52 6.42, 15.23

- ൠ⛩˖IARL 10.72 10.44 2.66 0.007 10.58 - 10.85 6.42, 15.23

- ൠ⛩˖ILWY 10.54 10.21 3.25 <0.001 10.44 - 10.65 6.42, 15.23

18:1 ⋩䞨 - ൠ⛩˖ARNE 26.34 25.35 3.93 <0.001 26.16 - 26.62 11.30, 43.27

- ൠ⛩˖IARL 22.91 21.95 4.41 0.002 22.84 - 22.98 11.30, 43.27

- ൠ⛩˖ILWY 23.58 23.24 1.44 0.043 23.29 - 23.78 11.30, 43.27

18:2 ӊ⋩䞨 - ൠ⛩˖ARNE 57.94 59.56 -2.72 <0.001 57.61 - 58.13 41.35, 74.78

- ൠ⛩˖IARL 62.57 63.90 -2.09 <0.001 62.49 - 62.70 41.35, 74.78

- ൠ⛩˖ILWY 62.01 62.72 -1.13 0.005 61.68 - 62.32 41.35, 74.78

117 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅㺘 27. MON 87427 ઼ᑨ㿴ሩ➗ᒣ൷٬㺘⧠ࠪ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶≷ᙫ˄㔝

ањԕк䈅傼⛩ᮠ٬ᆈ൘ᱮ㪇ᙗᐞᔲ ㊭㋂≘ส䞨˄ᒢส%˅

㳻≘䞨 - ൠ⛩˖ARNE 0.29 0.27 6.48 0.043 0.28 - 0.29 0.11, 0.29 - ൠ⛩˖IARL 0.23 0.25 -7.29 0.018 0.22 - 0.23 0.11, 0.29

㊭㋂㜲㛚䞨˄ঐᙫ㜲㛚䞨%˅

18:3 ӊ哫⋩䞨 - ൠ⛩˖ARNE 1.15 1.19 -3.92 0.033 1.13 - 1.17 0.78, 1.52

- ൠ⛩˖IARL 1.24 1.20 3.35 0.014 1.22 - 1.26 0.78, 1.52

㊭㋂㔤⭏㍐˄mg/kg dwt˅

㔤⭏㍐ B2 - ൠ⛩˖ARNE 3.27 2.36 38.30 0.004 3.05 - 3.56 0, 4.47

- ൠ⛩˖IARL 1.41 1.93 -26.71 0.042 1.17 - 1.60 0, 4.47

118 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅㺘 27. MON 87427 ઼ᑨ㿴ሩ➗ᒣ൷٬㺘⧠ࠪ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶≷ᙫ˄㔝

অњ䈅傼⛩ᮠ٬ᆈ൘ᱮ㪇ᙗᐞᔲ

㊭㋂ᑨ㿴ᡀ࠶˄ᒢส%˅ ⻣≤ॆਸ⢙˄ൠ⛩˖IARL˅ 84.24 83.11 1.36 0.047 83.60 - 84.96 80.77, 89.46

≤࠶˄⒯ส%˅˄ൠ⛩˖IARL˅ 10.93 10.40 5.13 0.043 10.90 - 11.00 7.56, 14.80

㳻ⲭ䍘˄ൠ⛩˖IARL˅ 10.60 11.73 -9.64 0.019 9.91 - 11.35 5.79, 13.43

㊭㋂㓔㔤˄ᒢส%˅ ADF˄ൠ⛩˖ILWY˅ 3.78 3.05 23.75 0.020 3.33 - 4.27 1.84, 4.39

㊭㋂≘ส䞨˄ᒢส%˅ ㋮≘䞨˄ൠ⛩˖IARL˅ 0.48 0.53 -9.19 0.033 0.45 - 0.49 0.24, 0.68

㜡≘䞨˄ൠ⛩˖IARL˅ 0.24 0.26 -5.95 0.012 0.24 - 0.25 0.14, 0.30

э≘䞨˄ൠ⛩˖IARL˅ 0.49 0.56 -11.21 0.037 0.46 - 0.51 0.24, 0.66

119 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅㺘 27. MON 87427 ઼ᑨ㿴ሩ➗ᒣ൷٬㺘⧠ࠪ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶≷ᙫ˄㔝

অњ䈅傼⛩ᮠ٬ᆈ൘ᱮ㪇ᙗᐞᔲ

㊭㋂≘ส䞨˄ᒢส%˅ 0.062 0.052 19.32 0.006 0.059 - 0.064 0.032, 0.069 㢢≘䞨˄ൠ⛩˖ARNE˅

㊭㋂㜲㛚䞨˄ঐᙫ㜲㛚䞨%˅

18:0 ⺜㜲䞨˄ൠ⛩˖ARNE˅ 2.17 2.04 6.43 0.002 2.16 - 2.17 0.87, 2.88

20:0 㣡⭏䞨˄ൠ⛩˖ARNE˅ 0.48 0.46 4.63 0.002 0.47 - 0.48 0.15, 0.67

22:0 ኡ㩞䞨˄ൠ⛩˖ARNE˅ 0.21 0.19 11.00 0.007 0.21 - 0.23 0, 0.32

㊭㋂ⸯ⢙䍘

䫉˄ᒢส%˅˄ൠ⛩˖ARNE˅ 0.0077 0.0067 14.03 0.024 0.0075 - 0.0079 0.0019, 0.0076

䬼˄mg/kg dw˅˄ൠ⛩˖IARL˅ 23.54 26.51 -11.20 0.010 22.45 - 24.61 11.46, 30.37

㊭㋂㔤⭏㍐˄mg/kg dwt˅

ਦ䞨˄ൠ⛩˖IARL˅ 0.36 0.45 -19.59 0.020 0.31 - 0.40 0.11, 0.61

120 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅㺘 27. MON 87427 ઼ᑨ㿴ሩ➗ᒣ൷٬㺘⧠ࠪ㔏䇑ᆖᱮ㪇ᙗᐞᔲⲴᡀ࠶≷ᙫ˄㔝

অњ䈅傼⛩ᮠ٬ᆈ൘ᱮ㪇ᙗᐞᔲ

㊭㋂ᣇ㩕ޫᡀ࠶˄ᒢส%˅

ỹᆀ㌆˄ൠ⛩˖ARNE˅ 0.11 0.13 -18.51 0.031 0.11 - 0.11 0.024, 0.29

ൠк䜘ᮤṚⲴᑨ㿴ᡀ࠶˄ᒢส%˅

⻣≤ॆਸ⢙˄ൠ⛩˖IARL˅ 86.46 84.12 2.78 0.029 86.21 - 86.75 80.13, 94.05

≤࠶˄⒯ส%˅˄ൠ⛩˖IARL˅ 69.90 74.71 -6.44 0.008 67.70 - 71.20 51.70, 86.22

㳻ⲭ䍘˄ൠ⛩˖IARL˅ 7.03 8.63 -18.59 0.037 6.75 - 7.40 1.34, 11.57

¹dwt=ᒢ䟽˗ൠ⛩˖ARNE = 䱯㛟㢢ᐎⲴ JacksonˈIARL = ⡡㦧ॾᐎⲴ JeffersonˈILWY = Ժ࡙䈪ԺᐎⲴ StarkǄ ² MON 87427 ௧㥹⭈㟖༴⨶Ǆ ᒣ൷٬Ѫᴰሿᒣᯩᒣ൷٬Ǆ ³ 4 ᑨ㿴ሩ➗о MON 87427 Ѫ䘁ㅹสഐޣ㌫ˈ௧㥹⭈㟖༴⨶Ǆ ୶ъॆ૱⿽Ⲵᒣ൷٬൘ 95%㖞ؑ䲀лⲴ 99%ᇩ䇨४䰤ˈྲ᷌ᱟ䍏ᮠቡ䇮ᇊѪ 0Ǆ 5

121 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㺘28. ਁ㺘᮷⥞઼ILSIᮠᦞᓃѝ⦹㊣ൠк䜘ἽṚ઼㊭㋂ᡀ࠶Ⲵᮠ٬㤳ത ㊭㋂㓴㓷ᡀ࠶ 1 ਁ㺘᮷⥞ѝᮠ٬㤳ത 2 ILSI ᮠᦞᓃѝᮠ٬㤳ത 3

㊭㋂㩕ޫᡀ࠶ਜ਼䟿

ᑨ㿴ᡀ࠶˄ᒢส%˅ ⚠࠶ 1.17 – 2.01a; 1.14 – 1.63b 0.616 – 6.282 ᣈਸ⻣≤ॆਸ⢙ 81.31 – 87.06a; 82.10 – 86.65b 77.4 – 89.5 ᙫ㜲㛚 2.95 – 4.40a; 3.16 – 4.23b 1.742 – 5.823 ≤࠶˄⒯ส%˅ 8.74 – 11.30a; 11.00 – 13.20b 6.1 – 40.5 㳻ⲭ䍘 8.27 – 13.33a; 8.55 – 12.19b 6.15 – 17.26

㓔㔤˄ᒢส%˅ 䞨ᙗ⍇⏔㓔㔤˄ADF˅ 1.82 – 4.48a; 1.14 – 4.41b 1.82 – 11.34 ѝᙗ⍇⏔㓔㔤˄NDF˅ 6.51 –12.28a; 6.08 – 10.36b 5.59 – 22.64 ᙫ㟣伏㓔㔤˄TDF˅ 10.65 – 16.26a; 10.24 – 14.56b 8.82 – 35.31

≘ส䞨˄ᒢส%˅ щ≘䞨 0.60 – 1.04a; 0.63 – 0.96b 0.439 – 1.393 ㋮≘䞨 0.34 – 0.52a; 0.32 – 0.50b 0.119 –0.639 ཙߜ≘䞨 0.52 – 0.78a; 0.56 – 0.77b 0.335 – 1.208 㜡≘䞨 0.19 – 0.26a; 0.20 – 0.26b 0.125 – 0.514 䉧≘䞨 1.54 – 2.67a; 1.62 – 2.44b 0.965 – 3.536 ⭈≘䞨 0.33 – 0.43a; 0.31 – 0.42b 0.184 – 0.539 㓴≘䞨 0.25 – 0.37a; 0.24 – 0.34b 0.137 – 0.434 ᔲӞ≘䞨 0.30 – 0.48a; 0.30 – 0.44b 0.179 – 0.692 Ӟ≘䞨 1.02 – 1.87a; 1.06 – 1.65b 0.642 – 2.492 䎆≘䞨 0.26 – 0.33a; 0.25 – 0.31b 0.172 – 0.668 㳻≘䞨 0.17 – 0.26a; 0.16 – 0.30b 0.124 – 0.468 㤟щ≘䞨 0.43 – 0.72a; 0.43 – 0.63b 0.244 – 0.930 㝟≘䞨 0.74 – 1.21a;0.72–1.11b 0.462 – 1.632 э≘䞨 0.39 – 0.67a; 0.40 – 0.60b 0.235 – 0.769 㣿≘䞨 0.29 – 0.45a; 0.29 – 0.39b 0.224 – 0.666 㢢≘䞨 0.047 – 0.085a; 0.040 – 0.070b 0.0271 – 0.215 䞚≘䞨 0.13 – 0.43a; 0.12 – 0.41b 0.103 – 0.642 㕜≘䞨 0.42 – 0.62a; 0.41 – 0.58b 0.266 – 0.855

122 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ㊭㋂㓴㓷ᡀ࠶ 1 ਁ㺘᮷⥞ѝᮠ٬㤳ത 2 ILSI ᮠᦞᓃѝᮠ٬㤳ത 3

㜲㛚䞨˄ঐᙫ㜲㛚䞨%˅ 16:0 ἅᾸ䞨 8.80 – 13.33a; 9.53 – 12.33b 7.94 – 20.71 18:0 ⺜㜲䞨 1.36 – 2.14 a; 1.28 – 2.13b 1.02 – 3.40 18:1 ⋩䞨 19.50 – 33.71a; 19.59 – 31.09b 17.4 – 40.2 18:2 ӊ⋩䞨 49.31 – 64.70a; 55.17 – 65.65b 36.2 – 66.5 18:3 ӊ哫⋩䞨 0.89 – 1.56a; 1.00 – 1.38b 0.57 – 2.25 20:0 㣡⭏䞨 0.30 – 0.49a; 0.29 – 0.42b 0.279 – 0.965 20:1 Ҽॱ⻣✟䞨 0.17 – 0.29a; 0.17 – 0.31b 0.170 – 1.917 22:0 ኡ㩞䞨 0.069 – 0.28a; 0.059 – 0.33b 0.110 – 0.349

ⸯ⢙䍘䫉˄ᒢส%˅ 0.0036 – 0.0068a; 0.0032 – 0.0070b 0.00127 – 0.02084 䬌˄mg/kg dwt˅ 1.14 – 3.43a; 1.29 – 4.16b 0.73 – 18.50 䫱˄mg/kg dwt˅ 14.17 – 23.40a; 14.37 – 24.66b 10.42 – 49.07 䭱˄ᒢส%˅ 0.091 – 0.14a; 0.095 – 0.14b 0.0594 – 0.194 䭠˄mg/kg dwt˅ 4.83 – 8.34a; 4.55 – 9.35b 1.69 – 14.30 ⼧˄ᒢส%˅ 0.24 – 0.37a; 0.26 – 0.38b 0.147 – 0.533 䫮˄ᒢส%˅ 0.29 – 0.39a; 0.32 – 0.45b 0.181 – 0.603 䬼˄mg/kg dwt˅ 16.78 – 28.17a; 18.12 – 30.44b 6.5 – 37.2

㔤⭏㍐˄mg/kg dw˅ ਦ䞨 0.19 – 0.35a; 0.22 – 0.42b 0.147 – 1.464 㔤⭏㍐ A[ –㜑㩍ঌ㍐] Not Available 0.19 – 46.81

a b 㔤⭏㍐ B1 [⺛㜪] 2.33 – 4.17 ; 2.71 – 4.78 1.26 – 40.00

a b 㔤⭏㍐ B2 [Ṩ哴㍐] 0.94 – 2.42 ; 1.46 – 2.81 0.50 – 2.36

a b 㔤⭏㍐ B3 [✏䞨] 15.07 – 32.38 ; 13.64 – 42.60 10.37 – 46.94

a b 㔤⭏㍐ B6 [ੑ૶㍐] 4.93 – 7.53 ; 4.01 – 8.27 3.68 – 11.32 㔤⭏㍐ E[–⭏㛢䞊] 5.96 – 18.44a; 2.83 – 15.53b 1.5 – 68.7

㊭㋂ᣇ㩕ޫഐᆀਜ਼䟿˄ᒢส%˅ Ἵ䞨 0.69 – 1.09a; 0.58 – 0.97b 0.111 – 1.570 ỹᆀ㌆ 0.079 – 0.22a; 0.028 – 0.15b 0.020 – 0.320

㊭㋂⅑⭏ԓ䉒ӗ⢙˄g/g dwt˅ 123 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ㊭㋂㓴㓷ᡀ࠶ 1 ਁ㺘᮷⥞ѝᮠ٬㤳ത 2 ILSI ᮠᦞᓃѝᮠ٬㤳ത 3

䱯兿䞨 1205.75 – 2873.05a; 820.14 – 2539.86b 291.9 – 3885.8 ሩ-俉䉶䞨 94.77 – 327.39a; 64.03 – 259.68b 53.4 – 576.2

ൠк䜘ἽṚ㩕ޫᡀ࠶

ᑨ㿴ᡀ࠶˄ᒢส%˅ ⚠࠶ 2.67–8.01a; 3.88 – 6.90b 1.527 – 9.638 ᣈਸ⻣≤ॆਸ⢙ 81.88 – 89.26a; 84.11 – 89.52b 76.4 – 92.1 ᙫ㜲㛚 1.28 – 3.62a; 0.20 – 2.33b 0.296 – 4.570 ≤࠶˄⒯ส%˅ 64.20 – 75.70a ; 71.40 – 78.00b 49.1 – 81.3 㳻ⲭ䍘 5.80 – 10.24a; 5.56 – 9.14b 3.14 – 11.57

㓔㔤˄ᒢส%˅ 䞨ᙗ⍇⏔㓔㔤˄ADF˅ 19.11 – 30.49a; 20.73 – 33.39b 16.13 – 47.39 ѝᙗ⍇⏔㓔㔤˄NDF˅ 27.73 – 49.62a; 31.81 – 50.61b 20.29 – 63.71

ⸯ⢙䍘˄ᒢส%˅ 䫉 0.12 – 0.33a; 0.17 – 0.41b 0.0714 – 0.5768 ⼧ 0.090 – 0.26a; 0.13 – 0.21b 0.0936 – 0.3704 1 dwt = ᒢ⢙䟽Ǆ 2 ਁ㺘᮷⥞ᶕⓀ˖a (Harrigan et al., 2009) [US 2006], b (Harrigan et al., 2009) [Chile 2006/2007]. 3 ILSI ᮠᦞᶕⓀ˖ILSI ֌⢙ᡀ࠶ᮠᦞᓃ˄Version 3.0˅ˈhttp://www.cropcomposition.org/ (ILSI, 2006)

124 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3.3.5 ᣍ⭕㍖ᣍᙝ MON 87427 Ⲵᨂޕᒿࡇ˄T-DNA˅ѝнਜ਼ᴹԫօᣇ⭏㍐ᣇᙗสഐ˄മ 6˅Ǆ MON 87427 Ⲵ䖜ॆ㺘䗮䖭փާᴹⲴ aadAˈ൘བྷ㛐ᵶ㧼઼ߌᵶ㧼࠶ᆀݻ䲶䗷〻ѝ⭘֌ᣇ⭏㍐䘹ᤙḷ䇠˄Fling et al., 1985˅Ǆ⭡Ҿ aadA ൘䖜ॆ⡷⇥˄T-DNA˅ 䗩⭼ᒿࡇѻཆˈഐ↔⋑ᴹ㻛䖜ॆࡠἽ⢙สഐ㓴Ǆ䙊䗷 Southern ᵲӔҏ䇱ᇎҶ MON 87427 нਜ਼䍘㋂僘ᷦᒿࡇ˄മ 17˅Ǆ 3.3.6 ሯӰ㊱ૂ伕૷ᆿޞᙝⲺެᆹᖧଃ ⭏⢙ᢰᵟ֌⢙ѝሬޕⲴ㳻ⲭሩӪ㊫伏⭘Ⲵᆹޘᙗ䇴ՠᗵ享㘳㲁ԕлࠐњ ᓄ⭘শਢ˗(c) оᐢ⸕ޘᙗ˗(b) 伏૱ᓄ⭘৺䮯ᵏᆹޘᯩ䶒˖(a) ׋փ⭏⢙Ⲵᆹ ∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃ㠤᭿㳻ⲭ઼ަᆳሩପңࣘ⢙ާᴹн㢟֌⭘Ⲵ⍫ᙗ㳻ⲭ ൘㔃ᶴкⲴ⴨լᙗ˗(d) ࣐✝༴⨶ਾⲴっᇊᙗ˗(e) ൘⁑ᤏ㛳⏢ѝⲴ⎸ॆᙗ˗ (f) ሩପңࣘ⢙Ⲵ▌൘∂ᙗǄԕкⲴᆹޘ䇴ԧ䍴ᯉᐢ㓿ሩ䘉Ӌᯩ䶒䘋㹼Ҷ䈖㓶䱀䘠ˈMON 87427 ⦹㊣ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭо䇨ཊᐢ㓿୶ъॆⲴᣇ㥹⭈㟖 ֌⢙ѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭаṧ˗൘䗷৫ 20 ᒤˈޘ⨳ᐢ㓿ᴹ 488 亩⅑ޣҾ CP4 EPSPS 㳻ⲭᆹޘᙗⲴᇑḕᡆ䇴ᇑˈᗇࠪⲴ㔃䇪൷䇔Ѫ CP4 EPSPS 㳻ⲭሩӪ ᓧ⋑ᴹн㢟ᖡ૽Ǆڕ⢙㊫઼ࣘ ⭡Ҿ MON 87427 ⦹㊣ᱟᣇ䲔㥹ࡲ֌⢙ˈ൘⭏ӗ֯⭘䗷〻ѝ䴰㾱䝽ਸ֯⭘ ԕ㥹⭈㟖Ѫᴹ᭸ᡀ࠶Ⲵ䲔㥹ࡲǄ൘ᯠⲴᣇ䲔㥹ࡲᙗ⣦୶ъॆ⿽Ἵѻࡽˈ䜭Պ᤹ ➗㢟ྭߌъ㿴㤳˄Good Agricultural Practices ˈGAP˅ḷ߶䘋㹼⭠䰤䈅傼ˈ᤹ ➗᧘㦀ࡲ䟿઼ᯩ⌅൘֌⢙кⴤ᧕௧ᯭ䲔㥹ࡲˈ࠶᷀⺞ᇊⴤ᧕᭦㧧Ⲵߌӗ૱৺ަ ࣐ᐕ㓴࠶ѝⲴ䲔㥹ࡲ↻⮉≤ᒣǄ䲔㥹ࡲ൘㊭㋂н਼㓴࠶ѝ↻⮉≤ᒣਟ㜭ᆈ൘ᐞ ᔲˈ൘ḀӋ㓴࠶ѝ⴨ሩᇼ䳶ˈ㘼൘ަᆳ㓴࠶ѝ⴨ሩ䖳ቁǄ࣐ᐕ㓴࠶ѝⲴ⴨ሩ↻ ⮉⎃㕙≤ᒣ⭘⎃㕙㌫ᮠ˄concentration factors, CF˅㺘⽪ˈ䇑㇇ޜᔿྲл˖CF= ࣐ᐕ㓴࠶↻⮉≤ᒣ/ᵚ࣐ᐕ㊭㋂↻⮉≤ᒣǄ

MON 87427 Ⲵ䳴ᙗಘᇈѝ CP4 EPSPS 㳻ⲭ㺘䗮䟿ᶱվˈн㜭㙀ਇ㥹⭈㟖䲔㥹ࡲǄ൘⦹㊣ᵲӔ⿽ࡦ⿽䗷〻ѝˈ⭘ਜ਼ᴹ MON 87427 䖜ॆփⲴ⦹㊣㠚Ӕ㌫֌⇽ ᵜˈُࣙҾ㥹⭈㟖䲔㥹ࡲⲴ௧ᯭӗ⭏䳴ᙗн㛢ˈ⭘ਜ਼ᴹ NK603ǃMON 88017 ㅹ ަᆳᣇ䲔㥹ࡲ䖜ॆփⲴ㠚Ӕ㌫֌⡦ᵜˈ⭏ӗⲴᵲӔ⿽ MON 87427 × NK603 ᡆ ❦MON 87427 × MON 88017 ⭘Ҿ⭏ӗ䬰୞Ǆ䘉ӋᵲӔ⿽Ⲵṭษ䗷〻ѝˈߌ≁׍ Պ᤹➗⴨਼Ⲵ㥹⭈㟖ḷㆮ᧘㦀ᯩᔿ௧ᯭ䲔㥹ࡲǄ 2008 ᒤ൘㖾ഭ⦹㊣⿽Ἵ४ 6 њᐎǃ6 њ䈅傼⛩ሩਜ਼ᴹ MON 87427 䖜ॆһԦ Ⲵ⦹㊣ᵲӔ⿽ MON 87427 × NK603 䘋㹼⭠䰤䈅傼ˈ᤹➗ḷㆮ᧘㦀֯⭘ᯩ⌅௧ᯭ 㥹⭈㟖ˈỰ⍻࠶᷀Ҷ᭦㧧Ⲵ⦹㊣㊭㋂ѝ㥹⭈㟖৺ަԓ䉒ӗ⢙≘⭢ส⼧䞨˄AMPA˅ Ⲵ↻⮉≤ᒣ˄㿱ᢰᵟᣕ੺ 16a˅ˈഭ䱵伏૱⌅ިငઈՊ˄Codex˅൘⦹㊣кᡰ䇮 ᇊⲴ㥹⭈㟖ᴰབྷ↻⮉䲀䟿˄MRL˅ਚवᤜ㥹⭈㟖Ǆк䘠 MON 87427 ᵲӔ⿽㥹⭈ 㟖ⲴỰ⍻㔃᷌㿱л㺘ˈ㺘ѝ MON 87427 ᵲӔ⿽⦹㊣㥹⭈㟖↻⮉䟿ⲴỰ⍻㔃᷌о ࡙⭘ㅜањᣇ㥹⭈㟖⦹㊣ GA21 ᔪ・Ⲵส⹰ᮠᦞสᵜа㠤ˈ䜭վҾ Codex MRL

125 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ٬Ǆ㘼สҾ GA21 䘋㹼Ⲵ࣐ᐕ㓴࠶↻⮉⹄ウᔪ・Ҷ䈕㊫⦹㊣⋩ǃ⦹㊣㊹Ⲵ↻⮉ᮠ ᦞˈࡇҾл㺘Ǆ 2016 ᒤ 4 ᴸˈ൘㖾ഭሩ MON 87427 × NK603 ⦹㊣˄㥹⭈㟖௧ᯭ༴⨶˅઼ᑨ 㿴⦹㊣ MPA640B˄ᵚ௧ᯭ༴⨶˅䘋㹼Ҷ䲔㥹ࡲ↻⮉≤ᒣⲴ࠶᷀ˈੁࠪ㤇ਾⲴ⦹ ㊣௧ᯭ䗷䟿˄~2×˅Ⲵ㥹⭈㟖䲔㥹ࡲ MON 79789ˈᴰᲊѪ V8 ᵏᡆṚ儈 48 㤡ረǄ MON 79789 Ⲵ௧ᯭᙫࡲ䟿Ѫ 4.5 lb a.e./㤡әˈ㘼㖾ഭሩਜ਼ᴹ NK603 ᡀ࠶ⲴᵲӔ ㊣ࡦᇊⲴᴰབྷݱ䇨㤇ਾ௧ᯭࡲ䟿Ѫ 2.25 lb a.e./ 㤡әǄ᭦䳶ࡠⲴ⦹㊣ἂ⭡㚄ਸ⦹ ᭦ࢢᵪ㝡㋂ˈᡰᗇ㊭㋂㖞Ҿ 4 њ㺻ᆀѝˈ⇿㺻㓖 50 ⻵⦹㊣㊭㋂Ǆ㊭㋂㓿䗷␵⌱ǃ ᥁䘹ਾ࠶ᡀࠐ㓴ԕ༷⹄⼘࣐ᐕˈሶ␵⌱ਾⲴᆼᮤ㊭㋂䘋㹼≤࠶䈳㢲ˈ㠣ਜ਼≤䟿㓖 21%Ǆ⭘ⴈᔿ⼘㊹ᵪ㊹⺾⦹㊣㋂ˈ᭦䳶⼘ࠪⲴᒢ㊹ˈ㖞Ҿߧ߫㇡ѝԕ༷࠶᷀Ǆ㜊 ң䜘࠶㓿仾ᒢǃ࣐✝ǃ঻⡷ǃ└ㆋㅹ᫽֌ˈԕᐢ✧㨳ਆˈӾ㜊㣭㊹ѝ࠶⿫ࠪ㋇ᨀ ⦹㊣⋩Ǆ㋇ᨀ⋩㓿㋮ࡦǃ┲ⲭ઼৫㠝˄RBD˅༴⨶ˈᗇࡠ㋮⛬ RBD ⦹㊣⋩ˈ㖞 Ҿߧ߫㇡ѝԕ༷࠶᷀Ǆ࡙⭘ᑖᴹ⭥⿫ᆀॆ௧䴮ಘⲴ LC-MS/MS ㌫㔏⍻ᇊࡍ࿻⦹ ㊣㊭㋂৺ަ࣐ᐕ㓴࠶ѝⲴ㥹⭈㟖઼ AMPA ↻⮉≤ᒣǄ࠶᷀㔃᷌㿱л㺘ˈ䈖㓶⹄ ウᣕ੺䈧㿱ᵜ⭣䈧䍴ᯉл޼ᢰᵟᣕ੺ 16bǄ ഭ䱵伏૱⌅ިငઈՊ˄Codex˅൘⦹㊣кᡰ䇮ᇊⲴ㥹⭈㟖ᴰབྷ↻⮉䲀䟿˄MRL˅ ਚवᤜ㥹⭈㟖Ǆк䘠 MON 87427 × NK603 ᵲӔ⿽⦹㊣ѝ㥹⭈㟖ⲴỰ⍻㔃᷌㿱л 㺘ˈ㺘ѝ MON 87427 ᵲӔ⿽⦹㊣㥹⭈㟖↻⮉䟿ⲴỰ⍻㔃᷌䜭䘌վҾ Codex MRL ⦹٬Ǆ㺘ѝഭ䱵伏૱⌅ިငઈՊ˄Codex˅ᡰ䇮・Ⲵᴰབྷ↻⮉䲀䟿ᴰࡍᱟ൘ᑨ㿴㊣к䇮ᇊⲴǄ൘ᣇ㥹⭈㟖⦹㊣кⲴ↻⮉䈅傼㔃᷌㺘᰾ˈ䲔㥹ࡲ㥹⭈㟖Ⲵ↻⮉≤ᒣ վҾ Codex 䇮ᇊⲴ MRLǄഐ↔⧠ᴹⲴ Codex MRL ᰒ䘲⭘Ҿᑨ㿴⦹㊣ˈҏ䘲⭘ Ҿᣇ㥹⭈㟖䲔㥹ࡲ⦹㊣Ǆྲ᷌⦹㊣࣐ᐕ㓴࠶Ự⍻ࡠⲴ↻⮉䟿≤ᒣ᰾ᱮ儈Ҿ㊭㋂к ٬Ǆն⭡Ҿ⦹㊣ Codex ᡆ䇨Պ䪸ሩ࣐ᐕ㓴࠶䇮ᇊу䰘Ⲵ MRLˈ٬ 䇮ᇊⲴ MRL ˈ˅5 ppm˄٬ ǃ⦹㊣㊹ㅹ㓴࠶ѝỰ⍻ࡠⲴ㥹⭈㟖Ⲵ↻⮉䟿䘌վҾ㊭㋂кⲴ MRL⋨ ٬Ǆ ഐ↔⋑ᴹу䰘䇮・⦹㊣㊹ǃ⦹㊣⋩Ⲵ MRL ߌ㦟ᴰབྷ↻⮉䲀䟿˄MRL˅ᱟᤷ伏૱ᡆߌӗ૱޵䜘ᡆ㺘䶒⌅ᇊݱ䇨Ⲵߌ㦟 ᴰབྷ⎃ᓖˈԕ⇿ॳݻ伏૱ᡆߌӗ૱ѝߌ㦟↻⮉Ⲵ∛ݻᮠ㺘⽪˄mg/kg ᡆ ppm˅Ǆ ߌ㦟ᴰབྷ↻⮉䲀䟿˄MRL˅ᱟ䙊䗷ሩߌ㦟䘋㹼ᒯ⌋Ⲵ∂⨶ᆖ䈅傼઼⴨ሩҾᴍ䵢 ᓧӗ⭏ڕ䇱нՊሩӪ㊫؍䟿Ⲵ㟣伏仾䲙䇴ՠˈ⺞ᇊⲴਟ᧕ਇⲴߌ㦟↻⮉≤ᒣˈԕ н㢟ᖡ૽Ǆߌ㦟ᴰབྷ↻⮉䲀䟿ᇎ䱵кᱟߌ㦟ਸ⨶֯⭘઼ߌӗ૱䍨᱃ᡰ䴰㾱䚥ӾⲴ ḷ߶ǄᴹӋഭᇦ׍ᦞ⌅ᖻ⌅㿴Ⲵ㾱≲ˈՊࡦᇊᵜഭߌӗ૱ᴰབྷ↻⮉䲀䟿ḷ߶ˈ㘼 Codex MRL ֌Ѫഭ䱵伏૱⌅ިငઈՊ伏૱ḷ߶Ⲵа䜘࠶ˈ⭡ Codex ߌ㦟↻⮉င ઈՊ׍ᦞ FAO/WHO ߌ㦟↻⮉㚄ᑝՊ䇞˄JMPR˅Ⲵᔪ䇞ࡦᇊⲴǄJMPR ᱟ⭡㚄 ਸഭ㋞ߌ㓴㓷઼ц⭼ছ⭏㓴㓷уᇦޡ਼㓴ᡀⲴ、ᆖ亮䰞㓴㓷ˈJMPR 䙊䗷ሩߌ㦟 ˅ᆖᮠᦞ઼↻⮉ᮠᦞ䘋㹼䇴ԧˈ׍ᦞ䇴ԧ㔃䇪ˈੁഭ䱵伏૱⌅ިငઈՊ˄Codex⨶∂ ᔪ䇞ᴰབྷ↻⮉䲀䟿ḷ߶˄MRL˅Ǆ ᙫѻˈ䙊䗷↻⮉䈅傼ˈ࠶᷀Ự⍻Ҷ MON 87427 × NK603 ⦹㊣ᵲӔ⿽ᡰ᭦ 㧧㊭㋂Ⲵ㥹⭈㟖↻⮉≤ᒣǄ൘㊭㋂ѝỰ⍻ࡠⲴᴰ儈↻⮉䟿䘌վҾ Codex ൘ᑨ㿴 ⦹㊣к䇮・Ⲵᴰབྷ↻⮉䲀䟿≤ᒣǄᣇ䲔㥹ࡲ⦹㊣㊭㋂৺ަ࣐ᐕᡀ࠶㥹⭈㟖↻⮉ ᮠᦞ㺘᰾ˈ⴨ሩҾ⦹㊣㊭㋂ˈ⦹㊣⋩ѝⲴ㥹⭈㟖↻⮉≤ᒣᶱབྷ䱽վˈ⦹㊣㊹↻ 126 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⮉≤ᒣ⴨∄㊭㋂ҏ〽ᴹ߿ቁǄᰐ䇪ᱟ⦹㊣㊹ǃ⦹㊣⋩䘈ᱟ㊭㋂ᵜ䓛ˈ㥹⭈㟖↻ ٬Ǆ ⮉䟿䜭䘌վҾ Codex ൘⦹㊣㊭㋂к䇮ᇊⲴ MRL 㺘 29. ᣇ㥹⭈㟖⦹㊣↻⮉䈅傼ᙫ㔃৺ Codex ᴰབྷ↻⮉䲀䟿≤ᒣ˄MRL˅ 㥹⭈㟖 GA21 ᵲӔ⿽ 1 MON 87427 × NK603 ᵲӔ⿽ 2 㓴㓷㊫ර Codex MRL ѝսᮠ˄㤳ത˅ ѝսᮠ˄㤳ത˅ ppm ppm ppm 0.11 0.04 ⦹㊣㊭㋂ 5 (<0.05 - 0.96) (0.01 - 0.13) <0.04 <0.053 ㋮⛬⦹㊣⋩ ᵚ䇮ᇊ (<0.02 – 0.32) (<0.05 - 0.1) 0.12 <0.054 ⦹㊣㊹ ᵚ䇮ᇊ (<0.05 – 1.1) (<0.05 - <0.05)

1 ਜ਼ᴹ䖜ॆһԦ GA21 Ⲵㅜаԓᣇ㥹⭈㟖⦹㊣ᵲӔ⿽Ⲵ㥹⭈㟖↻⮉ᮠᦞˈ⭘Ҿᔪ・䈕㊫ ᣇ㥹⭈㟖⦹㊣᭦㧧ӗ૱઼᭦㧧㊭㋂࣐ᐕ㓴࠶↻⮉ส⹰ᮠᦞ˗ 2 ㊭㋂↻⮉ᮠᦞᶕ㠚Ҿ 2008 ᒤ㖾ഭޝњ䈅傼⛩ѝԕ㥹⭈㟖ḷㆮࡲ䟿௧ᯭⲴ↻⮉䈅傼˄ᢰ ᵟᣕ੺ 16a˅ˈ㋮⛬⦹㊣⋩઼⦹㊣㊹ѝⲴ㥹⭈㟖↻⮉≤ᒣ䙊䗷 2016 ᒤ⍻ᗇⲴ⎃㕙㌫ᮠᣈ ㇇ᗇࡠ˄ᢰᵟᣕ੺ 16b˅˗ ⎃㕙㌫ᮠ=[࣐ᐕ㓴࠶ѝ࠶᷀⢙Ⲵ⎃ᓖ]/[RAC ㊭㋂ѝ࠶᷀⢙ Ⲵ⎃ᓖ]Ǆྲ᷌Ḁ⿽࣐ᐕ㓴࠶ѝⲴ↻⮉≤ᒣվҾ LOQ˄<0.05 ppm˅ᡆ㘵ᵚỰ⍻ࡠ࠶᷀⢙ˈ ࡉ⭘<0.05 ppm Ⲵ↻⮉≤ᒣᶕ䇑㇇䈕㓴࠶Ⲵ⎃㕙㌫ᮠǄ⭡Ҿᮠᆖ䇑㇇Ⲵഋ㠽ӄޕ㿴ࡉˈ Ǆޕᴹࠪ〽㺘ѝ੸⧠Ⲵ⎃㕙㌫ᮠਟ㜭Պо᡻ࣘ䇑㇇٬ 3 ⦹㊣⋩⎃㕙㌫ᮠ=⦹㊣⋩↻⮉䟿/⦹㊣㊭㋂↻⮉䟿=<0.05ppm/0.91ppm=<0.06˄⎃㕙㌫ᮠ ˅ᇊ٬䈧㿱ᢰᵟᣕ੺ 16b⍻ 4 ⦹㊣㊹⎃㕙㌫ᮠ=⦹㊣㊹↻⮉䟿/⦹㊣㊭㋂↻⮉䟿=0.83ppm/0.91ppm=0.91˄⎃㕙㌫ᮠ⍻ ˅ᇊ٬䈧㿱ᢰᵟᣕ੺ 16b

3.4 ṯᦤр䘦䇺ԭθ৸➝ᵢࣔ⌋ㅢॷпᶗᴿީḽ߼ࡈ࠼䖢ะഖὃ⢟ Ⲻᆿޞㅿ㓝 MON 87427㌫䟷⭘ᆹޘㅹ㓗ѪIⲴᑨ㿴⦹㊣ᵲӔ⿽Ѫਇփᒦ㓿㊫ර2Ⲵสഐ ᓧԕ৺ሩ⭏ڕ⢙᫽֌ᯩ⌅䖜ॆਾ㧧ᗇⲴǄབྷ䟿Ⲵ⹄ウ㺘᰾MON 87427ሩӪ㊫઼ࣘ ᘱ⧟ຳᆹޘᙗⲴᖡ૽оᑨ㿴䶎䖜สഐ⦹㊣ᱟа㠤ⲴǄṩᦞǉߌъ䖜สഐ⭏⢙ᆹޘ 䇴ԧ㇑⨶࣎⌅ǊㅜҼㄐㅜॱйᶑⲴ㿴ᇊˈ䖜สഐἽ⢙ᓄ䈕኎Ҿᆹޘㅹ㓗IǄ

127 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 4. 䖢ะഖὃ⢟ӝ૷ᆿޞᙝ䇺ԭ 4.1 ⭕ӝૂࣖᐛ⍱ࣞሯ䖢ะഖὃ⢟ⲺᆿޞᙝⲺᖧଃ ൘⦹㊣㊭㋂ԕ৺ਜ਼ᴹ⦹㊣㊭㋂Ⲵӗ૱Ⲵ࣐ᐕ䗷〻ѝˈሩሬޕ㳻ⲭⲴᆹޘᙗᖡ ૽ᴰབྷቡᱟ࣐✝༴⨶Ǆᇎ䱵кˈ࣐✝༴⨶൘⦹㊣㊭㋂৺ަӗ૱Ⲵ࣐ᐕ䗷〻ѝ䶎ᑨ Პ䙽Ǆഐ↔ˈ䇴ԧ࣐✝༴⨶ሩ CP4 EPSPS 㳻ⲭっᇊᙗⲴᖡ૽ᱟ䶎ᑨ䟽㾱ⲴǄ ྲ 3.3.2 䜘࠶ᡰ䘠ˈ䞦⍫ᙗᇎ傼㔃᷌㺘᰾ˈԕ 25ºCǃ37ºC ઼ 55ºC ࣐✝ 15 min ਾ㿲ሏࡠ CP4 EPSPS ⍫ᙗ〽ᴹ䱽վˈ࠶࡛䱽㠣ሩ➗Ⲵ 81%ǃ84%઼ 70%˗ն࣐ ✝ 30 min ਾˈ25ºC ༴⨶Ⲵ CP4 EPSPS ⍫ᙗ⋑ᴹਈॆˈ37ºC ༴⨶㓴Ⲵ䞦⍫䱽㠣ሩ ➗ⲴⲴ 88%ˈ㘼 55ºC ༴⨶㓴Ⲵ CP4 EPSPS 㺘⧠Ѫབྷᑵл䱽ˈӵѪሩ➗Ⲵ 25%Ǆ н䇪ᱟ༴⨶ 15 min 䘈ᱟ༴⨶ 30 minˈ75°C ઼ 95°C ⑙ᓖᶑԦлⲴ CP4 EPSPS 䞦 ⍫൷վҾỰ⍻䲀˄㺘 19 ઼㺘 20˅ǄSDS-PAGE ࠶᷀㔃᷌㺘᰾ˈ৲➗ḷ߶઼ሩ➗ ⢙वਜ਼аᶑ㺘㿲࠶ᆀ䟿㓖Ѫ 43.8 kDa ⲴѫᶑᑖˈሩᓄҾ CP4 EPSPS 㳻ⲭ˄മ 23 ⋐മ 24˅Ǆ൘ᡰᴹ༴⨶ᰦ䰤઼༴⨶⑙ᓖᶑԦлˈṧ૱Ⲵ 43.8 kDa ᶑᑖؑਧᕪᓖ઼ ᴹ᰾ᱮⲴ䱽վ˄മ 23ˈ⌣䚃 3~7˗മ 24ˈ⌣䚃 2~7˅Ǆ ഐ↔ˈ࣐✝༴⨶ӵՊ֯ CP4 EPSPS 㳻ⲭⲴ䞦⍫ᙗ䱽վˈնнՊ֯ CP4 EPSPS 㳻ⲭ䱽䀓ˈнՊᖒᡀᯠⲴ㳻ⲭ⡷⇥㘼ᑖᶕᯠⲴ▌൘㠤᭿仾䲙Ǆ ൘ަᆳᯩ䶒ˈ⭏ӗ઼࣐ᐕ⍫ࣘሩ MON 87427 ⦹㊣ӗ૱઼ᑨ㿴⦹㊣ӗ૱Ⲵᖡ ૽ᱟ⴨਼ⲴǄ࣐✝༴⨶൘аᇊ〻ᓖкՊ֯ᣇ㩕ޫഐᆀཡ৫⍫ᙗˈഐ↔Պᨀ儈䖜ส ഐἽ⢙࣐ᐕӗ૱ⲴᆹޘᙗǄ 4.2 䖢ะഖὃ⢟ӝ૷Ⲻどᇐᙝ оᑨ㿴⦹㊣⴨∄ˈMON 87427 ⦹㊣ѝሬޕањᶕⓀҾ൏༔ߌᵶ㧼Ⲵ cp4 epsps สഐ㺘䗮ⴂˈਟԕ㺘䗮 CP4 EPSPS 㳻ⲭǄ䲔↔ཆˈMON 87427 оᑨ㿴 ⦹㊣⋑ᴹӰѸн਼ǄCP4 EPSPS 㳻ⲭᱟ㣣俉᯿≘ส䞨ਸᡀ䙄ᖴкⲴањ䞦㳻ⲭˈ о MON 87427 ࣐ᐕӗ૱Ⲵっᇊᙗ⋑ᴹޣ㌫ǄਖཆˈCP4 EPSPS 㳻ⲭ൘࣐ᐕⲴ✝ ༴⨶䗷〻ѝՊཡ৫⍫ᙗ˄㿱к䘠 4.1 䜘࠶˅Ǆഐ↔ˈMON 87427 ࣐ᐕӗ૱Ⲵっᇊ ᙗоᑨ㿴⦹㊣࣐ᐕӗ૱Ⲵっᇊᙗ⋑ᴹᐞᔲǄ о MON 87427 ⦹㊣аṧˈMON 87427 Ⲵ࣐ᐕӗ૱ѝҏवਜ਼ CP4 EPSPS 㳻 ⲭǄ⁑ᤏ㛳⏢⎸ॆᇎ傼㔃᷌㺘᰾ˈ൘⁑ᤏ㛳⏢ᶑԦл 15 s ਾ CP4 EPSPS 㳻ⲭՊ 㻛⎸ॆ 95%ԕкˈ䞦⍫ᙗՊ䱽վ 90%ԕк˄㿱 3.3.2 䜘࠶˅Ǆ 4.3 䖢ะഖὃ⢟ӝ૷ૂ䖢ะഖὃ⢟ҁ䰪⧥ູᆿޞᙝⲺᐤᔸ MON 87427 ⦹㊣઼ᑨ㿴⦹㊣ѻ䰤ୟаⲴᐞ࡛൘Ҿ MON 87427 ѝሬޕ cp4 epsps สഐˈਟԕ㺘䗮ཆⓀ CP4 EPSP 㳻ⲭˈ֯ MON 87427 ਟԕᣇ㥹⭈㟖䲔㥹ࡲǄնབྷ䟿Ⲵᆹޘ䇴ԧ⹄ウ㔃᷌㺘᰾˖1˅MON 87427 ѝⲴཆⓀสഐᨂޕѪ অᤧ䍍ᨂޕˈਟԕっᇊ䚇Ր˗2˅CP4 EPSPS 㳻ⲭнՊሩ⧟ຳᑖᶕн࡙ᖡ૽˗3˅ MON 87427 Ⲵ㺘රᙗ⣦઼ߌ㢪ᙗ⣦оᑨ㿴⦹㊣⋑ᴹᱮ㪇ᐞᔲˈнՊ໎࣐䖜ਈѪ

128 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᴹᇣ⭏⢙Ⲵ仾䲙˗4˅ሩ䶎䶦ḷ⭏⢙Ⲵᖡ૽ਟԕᘭ⮕н䇑ˈнՊᖡ૽⧽〰઼☂ড ⢙⿽Ǆഐ↔ਟԕ䈤ሬޕⲴཆⓀ㳻ⲭሩ⧟ຳᆹޘᙗнՊӗ⭏н࡙ᖡ૽Ǆ⴨৽ˈ MON 87427 Ⲵᣇ䲔㥹ࡲ⢩ᙗ֯ߌ≁ᴹҶᴤཊᵲ㥹᧗ࡦ઼㙅֌ᯩᔿⲴ䘹ᤙˈਟԕ ⧟ᣔ؍㲰ˈᴹ࡙Ҿᴤྭൠץ䘋ݽ㙅ᢰᵟⲴਁኅˈᴹ࡙Ҿ߿ቁ≤൏⍱ཡ઼൏༔׳ ຳǄ MON 87427 ӗ૱൘⧟ຳᆹޘᙗᯩ䶒о MON 87427 ⋑ᴹӰѸн਼Ǆն MON 87427 ࣐ᐕӗ૱ѝ CP4 EPSPS 㳻ⲭ⍫ᙗՊ䱽վᡆїཡˈ਼ᰦ࣐ᐕӗ૱䟺 ᭮ࡠ⧟ຳⲴਟ㜭ᙗՊᴤվǄ 㔬кᡰ䘠ˈMON 87427 ⴤ᧕᭦㧧ӗ૱ᡆ㘵ަ࣐ᐕӗ૱䜭нՊ᭩ਈަሩ⧟ຳ ᆹޘᙗⲴᖡ૽ˈо MON 87427 ᵜ䓛ሩ⧟ຳᆹޘⲴᖡ૽ᱟаṧⲴǄ ᓭᖧଃⲺᐤᔸڛ㊱䖢ะഖὃ⢟ӝ૷ૂ䖢ะഖὃ⢟ҁ䰪ሯӰ 4.4 ྲࡽᡰ䘠ˈMON 87427 䖜ॆփ઼ᑨ㿴⦹㊣䰤Ⲵୟаᐞ࡛൘Ҿ MON 87427 ѝ cp4 epsps สഐˈਟԕ㺘䗮ཆⓀ CP4 EPSP 㳻ⲭˈ֯ MON 87427 ਟԕᣇ㥹⭈ ޕሬ 㟖䲔㥹ࡲǄ䲔Ҷᣇ䲔㥹ࡲᙗ⣦ˈMON 87427 ൘ߌ㢪ᙗ⣦ǃ㺘⧠රᙗ⣦઼㩕ޫᡀ ࠶ㅹᯩ䶒оᑨ㿴⦹㊣⋑ᴹᱮ㪇ᐞ࡛Ǆབྷ䟿Ⲵᆹޘ䇴ԧ⹄ウ㔃᷌㺘᰾˖1˅⦹㊣ᱟ 䮯ᵏ傟ॆ֌⢙ˈѪӪԜᡰ⟏⸕ˈާᴹ䮯ᵏᆹޘᓄ⭘ⲴশਢˈMON 87427 оѻާ ᴹᇎ䍘ㅹ਼ᙗ˗2˅MON 87427 ѝཆⓀᨂޕᒿࡇѪঅᤧ䍍Ⲵᆼᮤᨂޕˈਟԕっᇊ 䚇Ր˗3˅CP4 EPSPS 㳻ⲭᱟἽ⢙઼ᗞ⭏⢙ѝᲞ䙽ᆈ൘Ⲵ EPSPS 㳻ⲭѝⲴа⿽ˈ 䶎ᑨ⴨լ˗4˅CP4 EPSPS оᐢ⸕Ⲵ∂㍐㳻ⲭ઼ᣇ㩕ޫഐᆀǃ㠤᭿㳻ⲭ൘ᒿࡇ઼ 㔃ᶴкнާ⴨լᙗ˗5˅ሿ啐ᙕᙗ∂ᙗᇎ傼㔃᷌㺘᰾ CP4 EPSPS 㳻ⲭнާᴹ∂ᙗˈ NOAEL Ѫ㠣ቁ 2000 mg/kg/ཙˈMOE 儈䗮 2.76 × 106˗6˅൘⁑ᤏ㛳⏢ᶑԦл 15 s ਾ 95%ԕкⲴ CP4 EPSPS 㳻ⲭՊ㻛⎸ॆᦹ˗7˅࣐✝༴⨶Պ֯ CP4 EPSPS 㳻ⲭ ཡ⍫նнՊ䱽䀓˗8˅MON 87427 оᑨ㿴⦹㊣൘㩕ޫᡀ࠶кᇎ䍘ㅹ਼˗9˅ MON 87427 ѝ CP4 EPSPS 㳻ⲭоަᆳᐢ㓿ᢩ߶୶ъॆᓄ⭘Ⲵ䇨ཊަᆳᣇ㥹⭈㟖 ֌⢙˄ྲ NK603 ⦹㊣ǃ40-3-2 བྷ䉶઼ MON89788 བྷ䉶ㅹ˅Ⲵ CP4 EPSPS 㳻ⲭ⴨ ᓧᑖᶕ仾䲙ǄڕǄഐ↔ˈMON 87427 нՊሩӪ㊫਼ MON 87427 ӗ૱Ⲵ࣐ᐕ䗷〻ਟ㜭ᆈ൘࣐✝༴⨶Ǆ࣐✝༴⨶Պሬ㠤 CP4 EPSPS 㳻ⲭཡ⍫ˈഐ↔нՊ໎࣐ᡆ᭩ਈ MON 87427 ӗ૱ⲴᆹޘᙗǄ ᓧڕᙫѻˈMON 87427 ⴤ᧕᭦㧧ӗ૱ᡆ㘵ަ࣐ᐕӗ૱䜭нՊ᭩ਈަሩӪ㊫ ᓧⲴᖡ૽ᱟаṧⲴǄڕᙗˈо MON 87427 ᵜ䓛ሩӪ㊫ޘⲴᆹ 4.5 ৸➝ᵢࣔ⌋ㅢॷ഑ᶗᴿީḽ߼ࡈ࠼䖢ะഖὃ⢟ӝ૷Ⲻᆿޞㅿ 㓝㔬кᡰ䘠ˈⴞࡽሩ⦹㊣ᡰ䟷⭘Ⲵ࣐ᐕᯩ⌅нՊᖡ૽ᣇ䲔㥹ࡲ䖜ॆփ MON 87427 Ⲵᆹޘᙗˈণ࣐ᐕᯩ⌅ሩӗ૱ᆹޘᙗᖡ૽Ⲵᆹޘㅹ㓗ᓄ䈕Ѫㅜ II ㊫Ǆ ᵜ亩ⴞⲴ䖜ॆਇփᆹޘㅹ㓗Ѫ Iˈഐ↔ṩᦞǉߌъ䖜สഐ⭏⢙ᆹޘ䇴ԧ㇑⨶࣎⌅Ǌ ㅜҼㄐㅜॱഋᶑⲴ㿴ᇊˈᵜ亩ⴞ䖜สഐἽ⢙Ⲵӗ૱Ⲵᆹޘㅹ㓗ӽѪ IǄ

129 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ϵࢩำ຤ 1. ằᇦࣹ. 1989. ѝഭߌъ、ᆖᢰᵟਢは. ѝഭߌъࠪ⡸⽮. 2. ୀ੟ᆷ. 1986. ѝഭ֌⢙ṭษਢは. ѝഭߌъࠪ⡸⽮. 3. 䰾з㤡઼ቩ㤡ॾ. 1992. ѝഭߌъਁኅਢ. ཙ⍕、ᆖᢰᵟࠪ⡸⽮. ᓧ⣦ߥᶑḕᣕ੺ѻҼ˄2002˅ˉ㟣伏оڕ㘏ࠔ㤡઼ᶘᲃݹ. 2006. ѝഭት≁㩕ޫо .4 㩕ޫ㍐ᩴޕ⣦ߥ. Ӫ≁ছ⭏ࠪ⡸⽮. 5. ѝഭߌᆖՊ䚇Ր䍴ⓀᆖՊ. 1994. ѝഭ֌⢙䚇Ր䍴Ⓚ. ѝഭߌъࠪ⡸⽮. 6. Adams, T.B., J. Doull, J.I. Goodman, I.C. Munro, P. Newberne, P.S. Portoghese, R.L. Smith, B.M. Wagner, C.S. Weil, L.A. Woods and R.A. Ford. 1997. The FEMA GRAS assessment of furfural used as a flavour ingredient. Food and Chemical Toxicology 35:739-751. 7. Alexander, M.P. 1980. A versatile stain for pollen fungi, yeast and bacteria. Stain Technology 55:13-18. 8. Alibhai, M.F. and W.C. Stallings. 2001. Closing down on glyphosate inhibition - With a new structure for drug discovery. Proceedings of the National Academy of Sciences of the United States of America 98:2944-2946. 9. Anderson, K.S. and K.A. Johnson. 1990. Kinetic and structural analysis of enzyme intermediates: Lessons from EPSP synthase. Chemical Reviews 90:1131-1149. 10. Anderson, W.P. 1996. Weed ecology. Pages 27-38 in Weed Science: Principles and Applications. Third Edition. West Publishing Company, St. Paul, Minnesota. 11. AOSA. 2007. Rules for testing seeds. Association of Official Seed Analysts, Lincoln, Nebraska. 12. Armstrong, C.L., C.E. Green and R.L. Phillips. 1991. Development and availability of germplasm with high Type II culture formation response. Maize Genetics Cooperation Newsletter 65:92-93. 13. Bakkeren, G., Z. Koukollkova-Nicola, N. Grimsley, and B. Hohn. 1989. Recovery of Agrobacterium tumefaciens T-DNA molecules from whole plants early after transfer. Cell 57: 847-857. 14. Baltazar, B.M., J. de Jesús Sánchez-Gonzalez, L. de la Cruz-Larios and J.B. Schoper. 2005. Pollination between maize and teosinte: An important determinant of gene flow in Mexico. Theoretical and Applied Genetics 110:519-526. 15. Barker, R.F., K.B. Idler, D.V. Thompson and J.D. Kemp. 1983. Nucleotide sequence of the T-DNA region from the Agrobacterium tumefaciens octopine Ti plasmid pTi15955. Plant Molecular Biology 2:335-350. 16. Barker, T., H. Campos, M. Cooper, D. Dolan, G. Edmeades, J. Habben, J. Schlusser, D. Wright and C. Zinselmeier. 2005. Improving drought tolerance in maize. Pages 173-253 in Plant Breeding Reviews. Volume 25. J. Janick (ed.). John Wiley & Sons, Inc., Hoboken, New Jersey. 17. Barry, G.F., G.M. Kishore, S.R. Padgette and W.C. Stallings. 2001. Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases. Patent 6,248,876, U.S. Patent Office, Washington, D.C. 18. Barry, G., G. Kishore, S. Padgette, M. Taylor, K. Kolaoz, M. Weldon, D. Re, D. Elchholtz, K. Fincher, and L. Hallas. 1992. Inhibitors of amino acid biosynthesis: Strategies for imparting glyphosate tolerance to crop plants. Pages 139-145 in Biosynthesis and molecular regulation of amino acids in plants, B.K. Singh, H.E. Flores 130 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 and J.C. Shannon, (eds.) American Society of Plant Physiologists. 19. Benfey, P.N. and N.-H. Chua. 1989. Regulated Genes in Transgenic Plants. Science 244: 174-181. 20. Benfey, P., L. Ren and N.-H. Chua. 1989. The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns. EMBO Journal 8: 2195-2202. 21. Bertolla, F. and P. Simonet. 1999. Horizontal gene transfers in the environment: Natural transformation as a putative process for gene transfers between transgenic plants and microorganisms. Research in Microbiology 150:375-384. 22. Bevan, M., W.M. Barnes and M.-D. Chilton. 1983. Structure and transcription of the nopaline synthase gene region of T-DNA. Nucleic Acids Research 11:369-385. 23. Boyer, J.S. and M.E. Westgate. 2004. Grain yields with limited water. Journal of Experimental Botany 55:2385-2394. 24. Brown, S.M. and C.G. Santino. 1997. Enhanced expression in plants. Patent 5,593,874, U.S. Patent Office, Washington, D.C. 25. Buchanan, B., W. Gruissem and R. Jones. 2000. Phenylpropanoid and phenylpropanoid-acetate pathway metabolites. Pages 1286-1289 in Biochemistry and Molecular Biology of Plants. American Society of Plant Biologists, Rockville, Maryland. 26. CaJacob, C.A., P.C.C. Feng, G.R. Heck, M.F. Alibhai, R.D. Sammons and S.R. Padgette. 2004. Engineering resistance to herbicides. Pages 353-372 in Hankbook of Plant Biotechnology. P. Christou and H. Klee (eds.). John Wiley & Sons, Ltd., Hoboken, New Jersey. 27. Callis, J., M. Fromm and V. Walbot. 1987. Introns increase gene expression in cultured maize cells. Genes & Development 1: 1183-1200. 28. Campos, H., M. Cooper, G.O. Edmeades, C. Löffler, J.R. Schussler and M. Ibañez. 2006. Changes in drought tolerance in maize associated with fifty years of breeding for yield in the U.S. Corn Belt. Maydica 51:369-381. 29. Chinnusamy, V., L. Xiong and J.-K. Zhu. 2005. Use of genetic engineering and molecular biology approaches for crop improvement for stress environments. Pages 47-107 in Abiotic Stresses: Plant Resistance Through Breeding and Molecular Approaches. M. Ashraf and P.J.C. Harris (eds.). The Haworth Press, Inc., Binghamton, New York. 30. Claassen, M.M. and R.H. Shaw. 1970. Water deficit effects on corn. II. Grain components. Agronomy Journal 62:652-655. 31. Codex Alimentarius. 2003. Guideline for the conduct of food safety assessment of foods derived from recombinant-DNA plants. CAC/GL 45-2003. Codex Alimentarius Commission, Joint FAO/WHO Food Standards Programme, Food and Agriculture Organization of the United Nations, Rome, Italy. ftp://ftp.fao.org/codex/Publications/Booklets/Biotech/Biotech_2003e.pdf [Accessed May 12, 2010]. 32. Comai, L., L.C. Sen, and D.M. Stalker. 1983. An altered aroA gene product confers resistance to the herbicide glyphosate. Science 221:370-371. 33. Crockett, L.J. 1977. Contents. Pages vii-ix in Wildly Successful Plants: A Handbook of North American Weeds. Macmillan Publishing Co., Inc., New York, New York. 34. Delannay, X., T.T. Bauman, D.H. Beighley, M.J. Buettner, H.D. Coble, M.S. DeFelice, C.W.Derting,T.J.Diedrick,J.L.Griffin,E.S.Hagood,F.G.Hancock,S.E.Hart,B.J. 131 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 LaVallee, M.M. Loux, L. W.E., K.W. Matson, C.K. Moots, E. Murdock, A.D. Nickell, M.D.K. Owen, E.H. Paschall, L.M. Prochaska, P.J. Raymond, D.B. Reynolds, W.K. Rhodes, F.W. Roeth, P.L. Sprankle, L.J. Tarochione, C.N. Tinius, R.H. Walker, L.M. Wax, H.D. Weigelt, and S.R. Padgette. 1995. Yield evaluation of a glyphosate-tolerant soybean line after treatment with glyphosate. Crop Sci. 35:1461-1467. 35. Depicker, A., S. Stachel, P. Dhaese, P. Zambryski and H.M. Goodman. 1982. Nopaline synthase: Transcript mapping and DNA sequence. Journal of Molecular and Applied Genetics 1:561-573. 36. Devine, M., S.O. Duke and C. Fedtke. 1993. Herbicide translocation. Pages 67-94 in Physiology of Herbicide Action. PTR Prentice Hall, Inc., Englewood Cliffs, New Jersey. 37. Dicke, F.F. and W.D. Guthrie. 1988. The most important corn insects. Pages 767-867 in Corn and Corn Improvement. Third Edition. G.F. Sprague and J.W. Dudley (eds.). American Society of Agronomy, Inc., Crop Science Society of America, Inc., and Soil Science Society of America, Inc., Madison, Wisconsin. 38. Doebley J.F. and H.H. Iltis. 1980. Taxonomy of Zea (Gramineae). II. A subgeneric classification with key to taxa. Amer. J. Botany 67:982-993 39. Dowd, P.F. 2001. Biotic and abiotic factors limiting efficacy of Bt corn in indirectly reducing mycotoxin levels in commercial fields. Journal of Economic Entomology 94:1067-1074. 40. Duvick, D.N. 2001. Biotechnology in the 1930s: The development of hybrid maize. Nature Reviews Genetics 2:69-74. 41. Earle, F.R., J.J. Curtis and J.E. Hubbard. 1946. Composition of the component parts of the corn kernel. Cereal Chemistry 23:504-511. 42. Ellstrand, N.C., L.C. Garner, S. Hegde, R. Guadagnuolo and L. Blancas. 2007. Spontaneous hybridization between maize and teosinte. Journal of Heredity 98:183-187. 43. Ensminger, M.E., J.E. Oldfield and W. W. Heinemann. 1990. Feeds and Nutrition (Second Edition). The Ensminger Publishing Co., Clovis, CA, USA. 44. Eyal, Y., C. Curie and S. McCormick. 1995. Pollen Specificity Elements Reside in 30 bp of the Proximal Promoters of Two Pollen-Expressed Genes. The Plant Cell Online 7: 373-384. 45. FAO/WHO. 1991. Strategies for Assessing the Safety of Foods Produced by Biotechnology. Report of a joint FAO/WHO Consultation. World Health Organization. Geneva, Switzerland. 46. FAO/WHO. 2001. Evaluation of allergenicity of genetically modified foods. Report of a joint FAO/WHO expert consultation on allergenicity of foods derived from biotechnology. Food and Agriculture Organization of the United Nations, Rome, Italy. 47. FAOSTAT. 2010a. World maize production quantity, 2009. Food and Agriculture Organization of the United Nations, Rome, Italy. http://faostat.fao.org/site/567/DesktopDefault.aspx?PageID=567#ancor [Accessed March 29, 2011]. 48. FAOSTAT. 2010b. World maize oil production quantity, 2009. Food and Agriculture Organization of the United Nations, Rome, Italy. http://faostat.fao.org/site/636/DesktopDefault.aspx?PageID=636#ancor [Accessed March 29, 2011]. 49. Fling, M.E., J. Kopf and C. Richards. 1985. Nucleotide sequence of the transposon Tn7 gene encoding an aminoglycoside-modifying enzyme, 3"(9)-O-nucleotidyltransferase. Nucleic Acids Research 13:7095-7106.

132 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 50. Franz, J.E., M.K. Mao and J.A. Sikorski. 1997. Glyphosate's molecular mode of action. Pages 521-535 in Glyphosate: A Unique Global Herbicide. American Chemical Society, Washington, D.C. 51. Galinat, W.C. 1988. The origin of corn. Pages 1-31 in Corn and Corn Improvement. Third Edition. G.F. Sprague and J.W. Dudley (eds.). American Society of Agronomy, Inc., Crop Science Society of America, Inc., and Soil Science Society of America, Inc., Madison, Wisconsin. 52. Gasser, C. 1989. Genetically engineering plants for crop improvement. Science 244:1293-1299. 53. Giza, P.E. and R.C.C. Huang. 1989. A self-inducing runaway-replication plasmid expression system utilizing the Rop protein. Gene 78:73-84. 54. Goldberg, R.B., T.P. Beals and P.M. Sanders. 1993. Anther development: Basic principles and practical applications. Plant Cell 5:1217-1229. 55. Gonzalez, J. and J. Corral. 1997. Pp 18-39. Teosinte distribution in Mexico. In Proceedings of a Forum: Gene Flow Among Maize Landraces, Improved Maize Varieties, and Teosinte: Implications for Transgenic Maize. Serratos, J.A., M.C. Willcox, and F. Castillo (eds.). INIFAP, CIMMYT and CNBA, Mexico. 56. Goodman, M.M. 1988. The history and evolution of maize. Critical Reviews in Plant Sciences 7:197-220. 57. Gould, F.W. 1968. Grasses and Man. Page 382 in Grass Systematics. McGraw Hill, New York, New York. 58. Hamilton, D.A., M. Roy, J. Rueda, R.K. Sindhu, J. Stanford and J.P. Mascarenhas. 1992. Dissection of a pollen-specific promoter from maize by transient transformation assay. Plant Molecular Biology 18:211-218. 59. Hammond, B. and R.L. Fuchs. 1998. Safety evaluation for new varieties of food crops developed through biotechnology. Pages 61-79 in Biotechnology and Safety Assessment, Second Edition. J.A. Thomas (ed.). Taylor & Francis, Philadelphia, Pennsylvania. 60. Hammond, B.G., V.L. Vicini, G.F. Hartnell, N.W. Naylor, C.D. Knight, E.H. Robinson, R.L. Fuchs, and S.R. Padgette. 1995. The feeding value of soybeans fed to rats, poultry, catfish, and dairy cattle is not altered by incorporation of glyphosate tolerance. J. Nutr. 126:717-727. 61. Hanway, J.J. 1966. How a corn plant develops. Special Report No. 48. Iowa State University of Science and Technology, Cooperative Extension Service, Ames, Iowa. 62. Harrigan, G.G., W.P. Ridley, K.D. Miller, R. Sorbet, S.G. Riordan, M.A. Nemeth, W. Reeves and T.A. Pester. 2009. The forage and grain of MON 87460, a drought-tolerant corn hybrid, are compositionally equivalent to that of conventional corn. Journal of Agricultural and Food Chemistry 57:9754-9763. 63. Harrison, L.A., M.R. Bailey, M.W. Naylor, J.E. Ream, B.G. Hammond, D.L. Nida, B.L. Burnette, T.E. Nickson, T.A. Mitsky, M.L. Taylor, R.L. Fuchs and S.R. Padgette. 1996. The expressed protein in glyphosate-tolerant soybean, 5-enolpyruvylshikimate-3-phosphate synthase from Agrobacterium sp. strain CP4, is rapidly digested in vitro and is not toxic to acutely gavaged mice. Journal of Nutrition 126:728-740. 64. Haslam, E. 1993. Introduction, commentary and overview. Pages 1-16 in Shikimic Acid: Metabolism and Metabolites. John Wiley and Sons, Inc., Chichester, England. 65. Herrmann, K.M. 1995. The shikimate pathway: Early steps in the biosynthesis of aromatic compounds. Plant Cell 7:907-919. 133 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 66. Hileman, R.E., A. Silvanovich, R.E. Goodman, E.A. Rice, G. Holleschak, J.D. Astwood and S.L. Hefle. 2002. Bioinformatic methods for allergenicity assessment using a comprehensive allergen database. International Archives of Allergy and Immunology 128:280-291. 67. Hinchee, M.A.W., S.R. Padgette, G.M. Kishore, X. Delannay, and R.T. Fraley. 1993. Herbicide-tolerant crops. Pages 243-263 in Transgenic Plants. Vol 1, S. Kung and W. Ray, (eds.) Academic Press, Orlando, F.L. 68. Hoffman, L. and A. Baker. 2010. Market issues and prospects for U.S. distillers' grains: Supply, use, and price relationships. U.S. Department of Agriculture, Economic Research Service, Washington, D.C. 69. Huang, M.-D., F.-J. Wei, C.-C. Wu, Y.-I.C. Hsing and A.H.C. Huang. 2009. Analyses of advanced rice anther transcriptomes reveal global tapetum secretory functions and potential proteins for lipid exine formation. Plant Physiology 149:694-707. 70. Huttner, S.L., C. Arntzen, R. Beachy, G. Breuning, E. Nester, C. Qualset, and A. Vidaver. 1992. Revising oversight of genetically modified plants. Bio/Technology 10: 967-971. 71. ILSI. 2004. Nutritional and safety assessment of foods and feeds nutritionally improved through biotechnology: An executive summary. Comprehensive Reviews in Food Science and Food Safety 3:38-104. 72. ILSI. 2006. Crop Composition Database Version 3.0. International Life Science Institute, Washington, D.C. http://www.cropcomposition.org/ [Accessed August 12, 2010]. 73. James, C. 2011. Global Status of Commercialized Biotech/GM Crops: 2011. ISAAA Briefs no. 43. ISAAA, Ithaca, New York. 74. Jefferson, R.A., T.A. Kavanagh and M.W. Bevan. 1986. -Glucuronidase from Escherichia coli as a gene-fusion marker. Proc. Natl. Acad. Sci. USA 83: 8447-8451. 75. Jones, S.M., C.F. Magnolfi, S.K. Cooke and H.A. Sampson. 1995. Immunologic cross-reactivity among cereal grains and grasses in children with food hypersensitivity. Journal of Allergy and Clinical Immunology 96:341-351. 76. Kay, R., A. Chan, M. Daly and J. McPherson. 1987. Duplication of CaMV 35S promoter sequences creates a strong enhancer for plant genes. Science 236:1299-1302. 77. Keeler, K.H. 1989. Can genetically engineered crops become weeds? Bio/Technology 7:1134-1139. 78. Kishore, G.M., and D.M. Shah. 1988. Amino acid biosynthesis inhibitors as herbicides. Annu. Rev. Biochem. 57:627-663. 79. Kishore, G., D. Shah, S. Padgette, G. della-Cioppa, C. Gasser, D. Re, C. Hironaka, M. Taylor, J. Wibbenmeyer, D. Eichholtz, M. Hayford, N. Hoffmann, X. Delannay, R. Horsch, H. Klee, S. Rogers, D. Rochester, L. Brundage, P. Sanders and R.T. Fraley. 1988. 5-enolpyruvylshikimate 3-phosphate synthase. From biochemistry to genetic engineering of gIyphosate tolerance. Pages 37-48 in Biotechnology for Crop Protection. P.A. Hedin, J.J. Menn, and R.M. Hollingworth (eds.). American Chemical Society, Washington, D.C. 80. Klee, H.J., Y.M. Muskopf and C.S. Gasser. 1987. Cloning of an Arabidopsis thaliana gene encoding 5-enolpyruvylshikimate-3-phosphate synthase: Sequence analysis and manipulation to obtain glyphosate-tolerant plants. Molecular and General Genetics 210:437-442. 81. Kumudini, S. and T. Tollenaar. 1998. Corn Phenology. Guelph University. http://www.plant.uoguelph.ca/research/homepages/ttollena/corn.htm [Accessed August 15, 2006] 134 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 82. Leath, M.N. and L.D. Hill. 1987. Economics: Production, marketing, utilization. Pages 210-219 in Corn: Chemistry and Technology. S.A. Watson and P.E. Ramstad (eds.). American Association of Cereal Chemists, St. Paul, Minnesota. 83. Liener, I.E. 1994. Implications of antinutritional components in soybean foods. Critical Reviews in Food Science and Nutrition 34:31-67. 84. Liener, I.E. 2000. Non-nutritive factors and bioactive compounds in soy. Pages 13-45 in Soy in Animal Nutrition. J.K. Drackley (ed.). Federation of Animal Science Societies, Savoy, Illinois. 85. Lingenfelter, D.D. and N.L. Hartwig. 2003. Introduction to weeds and herbicides. Pennsylvania State University Agricultural Research and Cooperative Extension, University Park, Pennsylvania. 86. Ma, J., D.S. Skibbe, J. Fernandes and V. Walbot. 2008. Male reproductive development: Gene expression profiling of maize anther and pollen ontogeny. Genome Biology 9: R181.1-R181.17. 87. Maize GDB. 2010. Stock data in MaizeGDB. Maize Genetics and Genomics Database. http://www.maizegdb.org/stock.php [Accessed October 4, 2010]. 88. Mangelsdorf, P.C. 1974. Corn: Its origin, evolution and improvement. Harvard University Press, Cambridge, Massachusetts. 89. Maynard, L.A., J.K. Loosli, H.F. Hintz and R.G. Warner. 1979. Animal Nutrition (Seventh Edition). McGraw Hill Book Co. New York, NY., USA. 90. Mazur, B. 1989. The development of herbicide resistant crops. Annu. Rev. Plant Physiol. Plant Mol. Biol. 40:441-478. 91. McPherson, J. and R. Kay. 1994. Method for enhanced expression of a protein. Patent 5, 359,142, 92. Meng, E. and J. Ekboir. 2001. Current and future trends in maize production and trade. Pages 35-44 in CIMMYT 1999-2000 World Maize Facts and Trends. Meeting World Maize Needs: Technological Opportunities and Priorities for the Public Sector, The International Maize and Wheat Improvement Center, Mexico, D.F. 93. Metcalfe, D.D., J.D. Astwood, R. Townsend, H.A. Sampson, S.L. Taylor and R.L. Fuchs. 1996. Assessment of the allergenic potential of foods derived from genetically engineered crop plants. Critical Reviews in Food Science and Nutrition 36:165-186. 94. Muenscher, W.C. 1980. Mallow family. Pages 310-314 in Weeds. Second Edition. Comstock Publishing Associates, Ithaca, New York. 95. NCGA. 2008. 2008 world of corn. National Corn Growers Association, Chesterfield, Missouri. http://www.ncga.com/files/pdf/WorldofCorn2008.pdf [Accessed December 1, 2010]. 96. Nida, D.L., S. Patzer, P. Harvey, R. Stipanovic, R. Wood, and R.L. Fuchs. 1996. Glyphosate-tolerant cotton: the composition of the cottonseed is equivalent to that of conventional cottonseed. J. Agric. Food Chem. 44:1967-1974. 97. Nielsen, K.M., J.D.v. Elsas and K. Smalla. 2000. Safety issues in antibiotic resistance marker genes in transgenic crops. Pages 146-162 in Proceedings of the 6th International Feed Production Conference, 27-28 November 2000, Piacenza, Italy. 98. NRC (National Research Council). 2001. Nutrient Requirements of Dairy Cattle (7th revised Ed.). National Academy Press, Washington D.C., USA.. 99. Odell, J.T., F. Nagy and N.-H. Chua. 1985. Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature 313:810-812.

135 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 100. OECD-FAO. 2008. Agricultural outlook 2008-2017. Organisation for Economic Co-operation and Development/Food Agriculture Organization of the United Nations, Paris, France. 101. OECD. 1993. Safety evaluation of foods derived by modern biotechnology: Concepts and principles. Organisation for Economic Co-operation and Development, Paris, France. 102. OECD. 1999. Consensus document on general information concerning the genes and their enzymes that confer tolerance to glyphosate herbicide. Series on harmonization of regulatory oversight in biotechnology No.10. ENV/JM/MONO(99)9. Organization of European Cooperation and Development. Paris, France. 103. OECD. 2002. Consensus document on compositional considerations for new varieties of maize (Zea mays): Key food and feed nutrients, anti-nutrients and secondary plant metabolites. ENV/JM/MONO (2002)25. Series on the Safety of Novel Foods and Feeds, No. 6. Organisation for Economic Co-operation and Development, Paris, France. 104. OECD. 2003a. Considerations for the safety assessment of animal feedstuffs derived from genetically modified plants. ENV/JM/MONO(2003)10. Series on the Safety of Novel Foods and Feeds No. 9. Organisation for Economic Co-operation and Development, Paris, France. 105. OECD. 2003b. Consensus document on the biology of Zea mays subsp. mays (maize). ENV/JM/MONO(2003)11. Series on Harmonisation of Regulatory Oversight in Biotechnology No.27. Organisation of Economic Co-operation and Development, Paris, France. 106. Padgette, S.R., G. Della-Cioppa, D.M. Shah, R.T. Fraley, and G.M. Kishore. 1989. Selective herbicide resistance through protein engineering. Pages 442-476 in Cell culture and somatic cell genetics of plants. Vol 6, J. Schell et al., (ed.) Academic Press, New York. 107. Padgette, S.R., D.B. Re, G.F. Barry, D.E. Eichholtz, X. Delannay, R.L. Fuchs, G.M. Kishore, and R.T. Fraley. 1995a. New Weed Control Opportunities: Development of glyphosate tolerant soybeans. in Herbicide Resistant Crops, S.O. Duke, (ed.) CRC Press, Boca Raton, F.L. 108. Padgette, S.R., N.B. Taylor, D.L. Nida, M.R. Bailey, J. MacDonald, L.R. Holden, and R.L. Fuchs. 1995b. The composition of glyphosate-tolerant soybean seeds is equivalent to that of conventional soybeans. J. Nutr. 126:702-716. 109. Padgette, S.R., D.B. Re, G.F. Barry, D.E. Eichholtz, X. Delannay, R.L. Fuchs, G.M. Kishore and R.T. Fraley. 1996. New weed control opportunities: Development of soybeans with a Roundup ReadyTM gene. Pages 53-84 in Herbicide-Resistant Crops: Agricultural, Environmental, Economic, Regulatory and Technical Aspects. S.O. Duke (ed.). CRC Press, Inc., Boca Raton, Florida. 110. Pariza, M.W. and E.A. Johnson. 2001. Evaluating the safety of microbial enzyme preparations used in food processing: Update for a new century. Regulatory Toxicology and Pharmacology 33:173-186. 111. Pasini, G., B. Simonato, A. Curioni, S. Vincenzi, A. Cristaudo, B. Santucci, A.D.B. Peruffo and M. Giannattasio. 2002. IgE-mediated allergy to corn: A 50 kDa protein, belonging to the Reduced Soluble Proteins, is a major allergen. Allergy 57:98-106. 112. Pastorello, E.A., L. Farioli, V. Pravettoni, M. Ispano, E. Scibola, C. Trambaioli, M.G. Giuffrida, R. Ansaloni, J. Godovac-Zimmermann, A. Conti, D. Fortunato and C. Ortolani. 2000. The maize major allergen, which is responsible for food-induced allergic reactions, is a lipid transfer protein. Journal of Allergy and Clinical Immunology 136 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 106:744-751. 113. Pauls, J.D. and D. Cross. 1998. Food-dependent exercise-induced anaphylaxis to corn. Journal of Allergy and Clinical Immunology 101:853-854. 114. Perry, T.W. 1988. Corn as a livestock feed. Pages 941-963 in Corn and Corn Improvement. Third Edition. G.F. Sprague and J.W. Dudley (eds.). American Society of Agronomy, Inc., Crop Science Society of America, Inc., Soil Science Society of America, Inc., Madison, Wisconsin. 115. Reynolds, T.L., M.A. Nemeth, K.C. Glenn, W.P. Ridley and J.D. Astwood. 2005. Natural variability of metabolites in maize grain: Differences due to genetic background. Journal of Agricultural and Food Chemistry 53:10061-10067. 116. Ritchie, S.W., J.J. Hanway and G.O. Benson. 1997. How a corn plant develops. Special Report No. 48. Iowa State University of Science and Technology Cooperative Extension Service, Ames, Iowa. 117. Rooney, L.W. and S.O. Serna-Saldivar. 1987. Food uses of whole corn and dry-milled fractions. Pages 399-429 in Corn: Chemistry and Technology. S.A. Watson and P.E. Ramstad (eds.). American Association of Cereal Chemists, St. Paul, Minnesota. 118. Rozelle, S. and C. Carter. 2001. China’s Corn Economy: A Report to the National Research Initiative Committee. University of California, Davis, California. http://agecon.ucdavis.edu/people/faculty/scott-rozelle/docs/ChinaCorn/chinaproject.php . 119. Russell, W.A. and A.R. Hallauer. 1980. Corn. Pages 299-312 in Hybridization of Crop Plants. W.R. Fehr and H.H. Hadley (eds.). American Society of Agronomy, Inc., and Crop Science Society of America, Inc., Madison, Wisconsin. 120. Salomon, S. and H. Puchta. 1998. Capture of genomic and T-DNA sequences during double-strand break repair in somatic plant cells. EMBO Journal 17:6086-6095. 121. Salvador, R.J. 1997. Maize. Pages 769-775 in Encyclopedia of Mexico: History, Society & Culture. M.S. Werner (ed.). Fitzroy Dearborn Publishers, Chicago, Illinois. 122. Sánchez González, J.J. and J.A. Ruiz Corral. 1997. Teosinte distribution in Mexico. Pages 18-39 in Gene Flow Among Maize Lndraces, Improved Maize Varieties, and Teosinte: Implications for Transgenic Maize. J.A. Serratos, M.C. Willcox, and F. Castillo (eds.). CIMMYT, Mexico, D.F. 123. Sánchez, J.J.G., T.A.Y. Kato, M.S. Aguilar, J.M.C. Hernández, A.R. López and J.A.C. Ruiz. 1998. Ditribucion y caracterizacion del teocintle. Libro Tecnico No. 2. del Instituto Nacioal de Investigaciones Forestales, Agricolas y Pecuarias (INIFAP), Mexico. 124. Schönbrunn, E., S. Eschenburg, W.A. Shuttleworth, J.V. Schloss, N. Amrhein, J.N.S. Evans and W. Kabsch. 2001. Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail. Proceedings of the National Academy of Sciences of the United States of America 98:1376-1380. 125. Schoper, J., R. Lambert, B. Vasilas and M. Westgate. 1987. Plant factors controlling seed set in maize: The influence of silk, pollen and ear-leaf water status and tassel heat treatment at pollination. Plant Physiology 83:121-125. 126. Scibilia, J., E.A. Pastorello, G. Zisa, A. Ottolenghi, B. Ballmer-Weber, V. Pravettoni, E. Scovena, A. Robino and C. Ortolani. 2008. Maize food allergy: a double-blind placebo-controlled study. Clinical and Experimental Allergy 38:1943-1949. 127. Setter, T.L., B.A. Flannigan and J. Melkonian. 2001. Loss of kernel set due to water deficit and shade in maize: Carbohydrate supplies, abscisic acid, and cytokinins. Crop Science 41:1530-1540. 137 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 128. Shaw, R.H. 1988. Climate requirement. Corn and Corn Improvement, 609-630. 129. Shah, D.M., R.B. Horsch, H.J. Klee, G.M. Kishore, J.A. Winter, N.E. Tumer, C.M. Hironaka, P.R. Sanders, C.S. Gasser, S. Aykent, N.R. Siegel, S.G. Rogers, and R.T. Fraley. 1986. Engineering herbicide tolerance in transgenic plants. Plant Mol. Biol. 223:478-481. 130. Shull, G.H. 1909. A pure line method of corn breeding. Am. Breeders Assoc. Rep. 5:51-59 131. Sidorov, V. and D. Duncan. 2009. Agrobacterium-mediated maize transformation: Immature embryos versus callus. Pages 47-58 in Methods in Molecular Biology: Transgenic Maize. M.P. Scott (ed.). Humana Press Inc, Totowa, New Jersey. 132. Silvanovich, A., M.A. Nemeth, P. Song, R. Herman, L.Tagliani and G.A. Bannon. 2006. The value of short amino acid sequence matches for prediction of protein allergenicity. Toxicological Sciences 90:252-258. 133. Sjoblad, R.D., J.T. McClintock and R. Engler. 1992. Toxicological considerations for protein components of biological pesticide products. Regulatory Toxicology and Pharmacology 15:3-9. 134. Smith, D.R. and D.G. White. 1988. Diseases of corn. Pages 687-766 in Corn and Corn Improvement. Third Edition. G.F. Sprague and J.W. Dudley (eds.). American Society of Agronomy, Inc., Crop Science Society of America, Inc., and Soil Science Society of America, Inc., Madison, Wisconsin. 135. Stalker, D.M., C.M. Thomas and D.R. Helinski. 1981. Nucleotide sequence of the region of the origin of replication of the broad host range plasmid RK2. Molecular and General Genetics 181:8-12. 136. Stallings, W.C., S.S. Abdel-Meguid, L.W. Lim, H.-S. Shieh, H.E. Dayringer, N.K. Leimgruber, R.A. Stegeman, K.S. Anderson, J.A. Sikorski, S.R. Padgette and G.M. Kishore. 1991. Structure and topological symmetry of the glyphosate target 5-enol-pyruvylshikimate-3-phosphate synthase: A distinctive protein fold. Proceedings of the National Academy of Sciences of the United States of America 88:5046-5050. 137. Steinrücken, H.C. and N. Amrhein. 1980. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthase. Biochemical and Biophysical Research Communications 94:1207-1212. 138. Steinrücken, H.C. and N. Amrhein. 1984. 5-enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumoniae 2. Inhibition by glyphosate [N-(phosphonomethyl)glycine]. European Journal of Biochemistry 143:351-357. 139. Sunilkumar, G., L. Mohr, E. Lopata-Finch, C. Emani and K.S. Rathore. 2002. Developmental and tissue-specific expression of CaMV 35S promoter in cotton as revealed by GFP. Plant Molecular Biology 50: 463-479. 140. Sutcliffe, J.G. 1979. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Pages 77-90 in Cold Spring Harbor Symposia on Quantitative Biology, Cold Spring Harbor, New York. 141. Takeda, S. and M. Matsuoka. 2008. Genetic approaches to crop improvement: Responding to environment and population changes. Nature Reviews Genetics 9:444-457. 142. Tanaka, L.G., J.M. El-Dahr and S.B. Lehrer. 2001. Double-blind, placebo-controlled corn challenge resulting in anaphylaxis. Journal of Allergy and Clinical Immunology 107:744. 143. Terada, R. and K. Shimamoto. 1990. Expression CaMV35S-GUS gene in transgenic rice 138 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 plants. Molecular and General Genetics 220: 389-392. 144. Thomas, K., G. Bannon, S. Hefle, C. Herouet, M. Holsapple, G. Ladics, S. MacIntosh and L. Privalle. 2005. In silico methods for evaluating human allergenicity to novel proteins: International Bioinformatics Workshop Meeting Report, 23-24 February 2005. Toxicological Sciences 88:307-310. 145. Thomas, K., M. Aalbers, G.A. Bannon, M. Bartels, R.J. Dearman, D.J. Esdaile, T.J. Fu, C.M. Glatt, N. Hadfield, C. Hatzos, S.L. Hefle, J.R. Heylings, R.E. Goodman, B. Henry, C. Herouet, M. Holsapple, G.S. Ladics, T.D. Landry, S.C. MacIntosh, E.A. Rice, L.S. Privalle, H.Y. Steiner, R. Teshima, R. van Ree, M. Woolhiser and J. Zawodny. 2004. A multi-laboratory evaluation of a common in vitro pepsin digestion assay protocol used in assessing the safety of novel proteins. Regulatory Toxicology and Pharmacology 39:87-98. 146. Tranel, D., A. Perdomo and A. Knapp. 2008. Tassel development events leading to pollen production: A timeline. Maydica 53:207-216. 147. Troyer, F. 1994. Breeding early corn. Pp 341-396. In Specialty Corns. Arnel R. Hallauer (ed.). CRC Press, Inc., Boca Raton, Florida. 148. Tu, H. 2011. Amended Report for MSL0023244: The Assembly of Databases Used for FASTA, BLAST and Sliding Window Searches in 2011. Monsanto Technical Report MSL0023308. St. Louis, Missouri. 149. Tuan, F.C. and T. Peng. 2001. Structural changes in China’s livestock and feed production: Trade implications. 45th Annual Conference of the Australian Agricultural and Resource Economics Society, 23-25 January 2001, Adelaide, South Australia. 150. U.S. EPA. 1996. Plant pesticide inert ingredient CP4 enolpyruvylshikimate-3-D and the genetic material necessary for its production in all plants. Federal Register 61:40338-40340. 151. USDA-APHIS. 2000. Request for extension of determination of nonregulated status to the additional regulated article: Roundup Ready corn line NK603. U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Washington, D.C. http://www.aphis.usda.gov/brs/not_reg.html [Accessed September 30, 2010]. 152. USDA-FAS. 2000. Permanent normal trade relations with China: What’s at stake for corn? May 2000. U.S. Department of Agriculture, Foreign Agricultural Service, Washington, D.C. 153. USDA-FAS. 2011a. World corn production. U.S. Department of Agriculture, Foreign Agricultural Service, Washington, D.C. http://www.fas.usda.gov/psdonline/psdResult.aspx [Accessed March 28, 2011]. 154. USDA-FAS. 2011b. Corn TY imports, major importers. U.S. Department of Agriculture, Foreign Agriculture Service, Washington, D.C. http://www.fas.usda.gov/psdonline/psdResult.aspx [Accessed March 28, 2011]. 155. USDA-FAS. 2011c. Corn TY exports, top grain exporters. U.S. Department of Agriculture, Foreign Agricultural Service, Washington, D.C. http://www.fas.usda.gov/psdonline/psdResult.aspx [Accessed March 28, 2011]. 156. USDA-FAS. 2011d. Corn TY exports, world exporters. U.S. Department of Agriculture, Foreign Agricultural Service, Washington, D.C. http://www.fas.usda.gov/psdonline/psdResult.aspx [Accessed March 28, 2011]. 157. Vega-Segovia, M.L. and R. Ferrera-Cerrato. 1996. Microorganisms del rizoplano del maíz y frijol inoculados con mutantes de Rhizobium y Azospirillum. In: J. Pérez-Moreno and R. Ferrera-Cerrato. (eds.) Avances de Investigación, Área de

139 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Microbiología de Suelos. PROEDAF-IRENAT, Colegio de Postgraduados. Montecillo, Estado de México, pp. 9-17. 158. von Braun, J. 2007. The world food situation: New driving forces and required actions. International Food Policy Research Institute, Washington, D.C. 159. Watson, S.A. 1982. Corn: Amazing maize. General properties. Pages 3-29 in CRC Handbook of Processing and Utilization in Agriculture. Volume 2. I.A. Wolff (ed.). CRC Press, Inc., Boca Raton, Florida. 160. Watson, S.A. 1988. Corn marketing, processing, and utilization. Pages 881-940 in Corn and Corn Improvement. Third Edition. G.F. Sprague and J.W. Dudley (eds.). American Society of Agronomy, Inc., Crop Science Society of America, Inc., Soil Science Society of America, Inc., Madison, Wisconsin. 161. Westgate, M.E., M.E. Otegui and F.H. Andrade. 2004. Physiology of the corn plant. Pages 235-271 in Corn: Origin, History, Technology, and Production. C.W. Smith, J. Betrán, and E.C.A. Runge (eds.). John Wiley & Sons, Inc., Hoboken, New Jersey. 162. White, P.J. and L.M. Pollak. 1995. Corn as a food source in the United States: Part II. Processes, products, composition, and nutritive values. Cereal Foods World 40:756-762. 163. Wilkes, H.G. 1967. Wild teosinte and its genetic isolation from maize. Pages 121-127 in Teosinte: the Closest Relative of Maize. The Bussey Institution of Harvard University, Cambridge, Massachusetts. 164. Wilkes, H.G. 1972. Maize and its wild relatives. Science 177:1071-1077. 165. Wilkes, H.G. 1989. Maize: Domestication, racial evolution, and spread. Pages 440-455 in Foraging and Farming: The Evolution of Plant Exploitation. D.R. Harris and G.C. Hillman (eds.). Unwin Hyman, Ltd., London, England. 166. Wilkinson, J.E., D. Twell and K. Lindsey. 1997. Activities of CaMV 35S and nos promoters in pollen: implications for field release of transgenic plants. Journal of Experimental Botany 48: 265-275. 167. Williamson, J.D., M.E. Hirsch-Wyncott, B.A. Larkins and S.B. Gelvin. 1989. Differential Accumulation of a Transcript Driven by the CaMV 35S Promoter in Transgenic Tobacco. Plant Physiology 90: 1570-1576. 168. Wu, T. D. and Watanabe, C. K. 2005. GMAP: a genomic mapping and alignment program for mRNA and EST sequences. Bioinformatics, 21(9):185 169. Yang, N.-S. and P. Christou. 1990. Cell type specific expression of a CaMV 35S-GUS gene in transgenic soybean plants. Developmental Genetics 11: 289-293. 170. Zambryski, P., A. Depicker, K. Kruger and H.M. Goodman. 1982. Tumor induction by Agrobacterium tumefaciens: Analysis of the boundaries of T-DNA. Journal of Molecular and Applied Genetics 1:361-370.

140 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ޝǃ⴨ޣ䱴Ԧ䍴ᯉ ⴮ީ䱺Ԭ䍺ᯏⴤᖋ ᓅਭ 䱺Ԭ䍺ᯏ 享⸷

1 ⴤⲺะഖⲺṮ㤭䞮ᓅࡍ਀ެ᧞ሲⲺ≞ะ䞮ᓅࡍ 142

2 ⴤⲺะഖф䖳։ᶺᔰⲺഴ䉧 143

3 ⴤⲺะഖфὃ⢟ะഖ㓺᮪ਾ਀ެ㺞䗴Ⲻ࠼ᆆỶ⎁ᡌ䢪ᇐ㔉 146 ᷒δPCR Ỷ⎁ȽSouthern ᵸӚ࠼᷆ȽNorthern ᡌ Western ࠼᷆㔉᷒ȽⴤⲺะഖӝ⢟㺞䗴㔉᷒ε

4 䖢ะഖᙝ⣬਀ӝ⢟ⲺỶ⎁ૂ䢪ᇐᢶᵥ 171

5 ਺䈋僂䱬⇫ᇗ᢯ҜⲺགྷদԬδ䘑ਙ䖢ะഖ⭕⢟⴪᧛⭩䈭ᆿ 174 ޞ䇷ҜⲺθᵢ亯уມߏε

6 ਺䈋僂䱬⇫Ⲻᆿޞᙝ䇺ԭ䈋僂ᙱ㔉ᣛ઀δ䘑ਙ䖢ะഖ⭕⢟ 174 ⴪᧛⭩䈭ᆿޞ䇷ҜⲺθᵢ亯уມߏε

7 䖢ะഖὃ⢟ሯ⭕ᘷ⧥ູᆿޞᙝⲺ㔲ਾ䇺ԭᣛ઀ 174

8 伕⭞ᆿޞᙝⲺ㔲ਾ䇺ԭᣛ઀θऻᤢ:Aεᗻ㾷Ⲻࣞ⢟∈⨼䈋 174 僂ᣛ઀χBε伕૷䗽ᮅᙝ䇺ԭ䈋僂ᣛ઀χCεф䶔䖢ะഖὃ ⢟∊䖹θެ㩛ޱᡆԳ਀ᣍ㩛ޱഖᆆ࠼᷆ᣛ઀ㅿ

9 䈛㊱䖢ะഖὃ⢟ള޻ཌ⭕ӝᓊ⭞Ᾰ߫ 182

10 ⭦䰪ⴇ᧝ᯯṾθऻᤢⴇ᧝ᢶᵥȽᣍᙝ⋱⨼᧠᯳Ƚ䮵ᵕ⧥ູ 268 ᭾ᓊⲺ⹊ガᯯ⌋ㅿ

11 ᇗḛᡶ䴶Ⲻެᆹ⴮ީ䍺ᯏ 269

䖢ะഖ⭕⢟൞䗉࠰ളᇬᡌ൦॰ᐨ㔅ݷ䇮֒Ѱ⴮ᓊ⭞䙊ᒬᣋ 271 12 ᭴ᐸ൰Ⲻ䇷᱄ᮽԬ

13 䗉࠰ളᇬᡌ൦॰㔅䗽〇ᆜ䈋僂䇷᱄ሯӰ㊱Ƚࣞὃ⢟ૂ⭕ᘷ 290 ⧥ູᰖᇩⲺ䍺ᯏ

⌞φ ѰҼф↙ᮽ॰࠼θᵢ䱺Ԭ䜞࠼ഴ㺞䠃᯦㕌ਭѰ䱺ഴ 1Ƚ䱺ഴ 2 ᡌ䱺㺞 1Ƚ 䱺㺞 2 ㅿȾ 141 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 1. ⴤⲺะഖⲺṮ㤭䞮ᓅࡍ਀ެ᧞ሲⲺ≞ะ䞮ᓅࡍ MON 87427 ⲴᨂޕᒿࡇབྷሿѪ 3746 bpˈ㘼 T-DNA ᨂޕᒿࡇབྷሿѪ 3681 bpǄ 䲔 T-DNA ཆˈ൘T-DNAⲴ5'ㄟ઼ 3'ㄟ࠶࡛ᴹ 41 bp઼ 24 bp Ⲵᨂޕˈ䘉ᱟ൘ T-DNA ᨂޕ䗷〻ѝ㠚㹼ᨂޕⲴᒿࡇǄT-DNA ѝⲴⴞⲴสഐ cp4 epsps ⲴབྷሿѪ 1368 bpǄ cp4 epsps ⲴṨ㤧䞨ᒿࡇ㿱䱴മ 1ˈ⴨ᖃҾ䱴മ 5˄MON 87427 ޘ䮯ᨂޕᒿࡇ઼ส 㘬 DNA ᒿࡇ˅ᡰ⽪Ⲵ 2995~4362 bp Ⲵս㖞Ǆחഐ㓴 MON 87427 㺘䗮Ⲵ CP4 EPSPS 㳻ⲭབྷሿ㓖Ѫ 47.6 kDˈਜ਼ᴹ 455 њ≘ส䞨ˈ ṩᦞⴞⲴสഐṨ㤧䞨ᒿࡇ᧘ሬⲴ CP4 EPSPS 㳻ⲭⲴ≘ส䞨ᒿࡇ㿱䱴മ 2Ǆ

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

䱴മ1. MON 87427ѝcp4 epspsสഐⲴṨ㤧䞨ᒿࡇ˄CBI˅ 1 MLHGASSRPA TARKSSGLSG TVRIPGDKSI SHRSFMFGGL ASGETRITGL 51 LEGEDVINTG KAMQAMGARI RKEGDTWIID GVGNGGLLAP EAPLDFGNAA 101 TGCRLTMGLV GVYDFDSTFI GDASLTKRPM GRVLNPLREM GVQVKSEDGD 151 RLPVTLRGPK TPTPITYRVP MASAQVKSAV LLAGLNTPGI TTVIEPIMTR 201 DHTEKMLQGF GANLTVETDA DGVRTIRLEG RGKLTGQVID VPGDPSSTAF 251 PLVAALLVPG SDVTILNVLM NPTRTGLILT LQEMGADIEV INPRLAGGED 301 VADLRVRSST LKGVTVPEDR APSMIDEYPI LAVAAAFAEG ATVMNGLEEL 351 RVKESDRLSA VANGLKLNGV DCDEGETSLV VRGRPDGKGL GNASGAAVAT 401 HLDHRIAMSF LVMGLVSENP VTVDDATMIA TSFPEFMDLM AGLGAKIELS 451 DTKAA

䱴മ2. ṩᦞMON 87427ѝcp4 epspsสഐⲴṨ㤧䞨ᒿࡇ᧘ሬⲴ CP4 EPSPS㳻ⲭⲴ≘ส䞨ᒿࡇ

142 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߙԅ෍ୖٲԅݮ࿙ူၻ඘ં .2 MON 87427 Ⲵ䖜ॆ䖭փѪ PV-ZMAP1043 䍘㋂ǄPV-ZMAP1043 䍘㋂Ⲵབྷሿ 㓖Ѫ 8.9 kbˈवਜ਼࠶࡛⭡ᐖǃਣ䗩⭼࠶ᔰⲴ T-DNAǄT-DNA वਜ਼ањᆼᮤⲴ cp4 epsps สഐ㺘䗮ⴂˈ䈕สഐ㺘䗮ⴂवᤜ e35S ੟ࣘᆀǃhsp70 Ⲵ޵ਜ਼ᆀǃCTP2 ਦ㔯փ䖜〫㛭ᒿࡇǃcp4 epsps 㕆⸱ᒿࡇ઼ nos 㓸→ᆀǄPV-ZMAP1043 䍘㋂ᡰव 䜘䚇ՐݳԦ≷ᙫҾ䱴㺘 1ˈަ䍘㋂മ䉡㿱䱴മ 3Ǆޘਜ਼Ⲵ PV-ZMAP1043 䍘㋂ᱟӪᐕᶴᔪਸᡀⲴˈަ僘ᷦᶕⓀҾབྷ㛐ᵶ㧼Ǆབྷ㛐ᵶ㧼 ൘⧟ຳ䟼ᰐᡰн൘ˈҏᆈ൘ҾӪ㊫ㅹ㜺Ἶࣘ⢙Ⲵ⎸ॆ㌫㔏ѝ˄Jefferson et al., 1986˅Ǆ⭡Ҿབྷ㛐ᵶ㧼Ⲵ䘉⿽⭏⢙ᆖ⢩ᙗˈа㡜䇔Ѫ⋑ᴹᗵ㾱ਖཆ䘋㹼仍ཆᆹޘ ᙗ࠶᷀ᡆᇎ傼Ǆ䈕㺘䗮䖭փнާᴹ仍ཆⲴ㠤⯵ᙗˈҏнਟ㜭╄ਈᡀѪާᴹ㠤⯵ᙗ Ⲵ䖭փˈሩӪ㊫઼ࣘ⢙ᱟᆹޘⲴǄ PV-ZMAP1043 䍘㋂кⲴᐖ䗩⭼ᒿࡇ઼ਣ䗩⭼ᒿࡇᶕⓀҾṩⱼߌᵶ㧼 ˄Agrobacterium tumefaciens˅˄Barker et al., 1983; Depicker et al., 1982; Zambryski et al., 1982˅Ǆ⇿њ䗩⭼ᒿࡇ਴वਜ਼ᴹ 24-25 bp ൘䖜ॆᰦਟԕਁ⭏ DNA ӔᦒⲴ 㕪࡫ս⛩Ǆᐖਣ䗩⭼ᒿࡇሶ T-DNA ઼䍘㋂僘ᷦᒿࡇ࠶ᔰǄ൘ PV-ZMAP1043 䍘㋂Ⲵ僘ᷦᒿࡇ४ฏ޵वᤜᴹ㓶㧼༽ࡦ䎧⛩˄ori V ઼ ori-pBR322˅ǃ㓶㧼䘹ᤙḷ ᤱ൘བྷ㛐ᵶ㧼ѝⲴᤧ䍍ᮠⲴᕅᆀ㳻ⲭ䱫䙿⢙Ⲵ㕆⸱४˄rop˅˄䱴؍઼ 䇠สഐ aadA 㺘 1˅Ǆн䇪ᱟ䘉ӋսҾ僘ᷦᒿࡇк䚇ՐݳԦ䘈ᱟ僘ᷦкⲴަᆳᒿࡇ൷⋑ᴹᮤਸ 䘋⦹㊣สഐ㓴˄䱴മ 10˅Ǆ

143 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 B-Left Border Region OR-ori V P-e35S

I-hsp70 Nsi I1719 Nco I1952 TS-CTP2 CS-rop T-DNA PV-ZMAP1043 Sph I2184 6 8946 bp

OR-ori-pBR322 CS-cp4 epsps

Nsi I3676 aadA T-nos Sph I3986 B-Right Border Region

䱴മ3. PV-ZMAP1043䍘㋂മ˄⽪ᵲӔ᧒䪸˅ кമѪُࣙҾߌᵶ㧼䖜ॆᯩ⌅ᗇࡠᣇ㥹⭈㟖䖜สഐ⦹㊣ MON 87427 ᰦᡰ˖⌘ ḷ᰾Ҷ䚇ՐݳԦ઼ח⭘㺘䗮䖭փ PV-ZMAP1043 Ⲵ⧟ᖒ䍘㋂മ䉡Ǆ䍘㋂മཆ Southern ᵲӔ࠶᷀ᡰ⭘䲀ࡦᙗ޵࠷䞦ս⛩˄㋇փ˅ˈSouthern ᵲӔ࠶᷀ᡰ⭘᧒䪸˄᧒ Ǆח䪸 1~7˅⴨ሩս㖞ḷҾ⧟ᖒമ޵

144 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴㺘1. PV-ZMAP1043䍘㋂ᡰਜ਼䚇ՐݳԦᾲ䘠䍘㋂ ѝ Ⲵ ˅⥞䚇ՐݳԦ ࣏㜭᧿䘠˄৲㘳᮷ ս㖞˄bp˅ T-DNA ṩⱼߌᵶ㧼 T-DNA ᐖ䗩⭼ᒿࡇˈ৲о T-DNA 䖜઼〫ᮤਸ B1-ᐖ䗩⭼ 1-442 ˄Barker et al., 1983˅ 䰤ᨂᒿࡇ 443-483 DNA ݻ䲶઼ᶴᔪ䴰㾱 P2-e35S वਜ਼ᴹ䟽༽໎ᕪ४ฏ˄Kay et al., 1987˅Ⲵ㣡ὠ㨌㣡ਦ⯵∂ 484-1104 ˄CaMV˅35S RNA Ⲵ੟ࣘᆀ˄Odelletal.,1985˅ 䰤ᨂᒿࡇ 1105-1125 DNA ݻ䲶઼ᶴᔪ䴰㾱 ⦹㊣✝◰㳻ⲭ 70 สഐⲴㅜањ޵ਜ਼ᆀ˄Brown and Santino, I3-hsp70 1126-1929 1997˅ 䰤ᨂᒿࡇ 1930-1953 DNA ݻ䲶઼ᶴᔪ䴰㾱㕆⸱ᤏই㣕 EPSPS 䞦ਦ㔯փ䖜〫㛭४ฏⲴสഐ ShkG Ⲵ䶦 TS4-CTP2 1954-2181 ḷᒿࡇ˄Herrmann, 1995; Klee et al., 1987˅ˈᕅሬ CP4 EPSPS 䖜〫ࡠਦ㔯փ 㓿ᇶ⸱ᆀՈॆⲴṩⱼߌᵶ㧼 CP4 㧼Ṛ aroA สഐ㕆⸱ᒿࡇˈ CS5- 2182-3549 㕆⸱ CP4 EPSPS 㳻ⲭ˄Barry et al., 2001; Padgette et al., cp4 epsps 1996˅ 䰤ᨂᒿࡇ 3550-3555 DNA ݻ䲶઼ᶴᔪ䴰㾱 ṩⱼߌᵶ㧼㜝㜲⻡ਸ䞦สഐ˄nos˅3'ㄟ䶎䖜ᖅ४ˈ㓸→䖜 T6-nos 3556-3808 ᖅᒦфᕅሬ mRNA Ⲵཊ㚊㞪㤧䞨ॆ˄Bevan et al., 1983˅ 䰤ᨂᒿࡇ 3809-3835 DNA ݻ䲶઼ᶴᔪ䴰㾱 ṩⱼߌᵶ㧼 T-DNA ᐖ䗩⭼ᒿࡇˈ৲о T-DNA 䖜઼〫ᮤਸ B-ਣ䗩⭼ 3836-4192 ˄Depicker et al., 1982; Zambryski et al., 1982˅ 䍘㋂僘ᷦ DNA 䰤ᨂᒿࡇ 4193-4328 DNA ݻ䲶઼ᶴᔪ䴰㾱䖜ᓗᆀ Tn7 Ⲵ≘ส㤧㊫ᣇ⭏㍐䫍ॆ䞦˄3''(9)-O-Ṩ㤧䞨䖜〫 aadA 4329-5217 䞦˅Ⲵ੟ࣘᆀǃ㕆⸱ᒿࡇ઼ ㄟ䶎䖜ᖅ४˄Fling et al., 1985˅ˈ ਟԕ֯㓶㧼ᣇᇴ᭮㓯㧼㍐઼䬮䴹㍐䰤ᨂᒿࡇ 5218-5747 DNA ݻ䲶઼ᶴᔪ䴰㾱 OR7-ori- pBR322 䍘㋂Ⲵ DNA ༽ࡦ䎧࿻⛩ˈ㔤ᤱབྷ㛐ᵶ㧼˄E. coli˅ 5748-6336 pBR322 ѝ䍘㋂Ⲵ༽ࡦ˄Sutcliffe, 1979˅ 䰤ᨂᒿࡇ 6337-6763 DNA ݻ䲶઼ᶴᔪ䴰㾱 ColE1 䍘㋂ѝᕅᆀ㳻ⲭ䱫䙿⢙Ⲵ㕆⸱ᒿࡇˈ㔤ᤱབྷ㛐ᵶ㧼 CS-rop 6764-6955 ˄E. coli˅ѝ䍘㋂Ⲵᤧ䍍ᮠ˄Giza and Huang, 1989˅ 䰤ᨂᒿࡇ 6956-8463 DNA ݻ䲶઼ᶴᔪ䴰㾱 ᒯᇯѫ㤳ത䍘㋂ RK2 Ⲵ༽ࡦ䎧࿻ս⛩ˈ㔤ᤱߌᵶ㧼 OR-ori V 8464-8860 ˄Agrobacterium˅ѝ䍘㋂༽ࡦ˄Stalker et al., 1981˅ 䰤ᨂᒿࡇ 8861-8946 DNA ݻ䲶઼ᶴᔪ䴰㾱 1 B–䗩⭼ᒿࡇ˗2 P–੟ࣘᆀ˗3 I–޵ਜ਼ᆀ˗4 TS –䖜〫㳻ⲭᒿࡇ˗5 CS –㕆⸱ᒿࡇ˗6 T– 㓸→ᆀ˗7 OR –༽ࡦ৏⛩ 145 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3. ⴤⲺะഖфὃ⢟ะഖ㓺᮪ਾ਀ެ㺞䗴Ⲻ࠼ᆆỶ⎁ᡌ䢪ᇐ㔉᷒ δPCR Ỷ⎁ȽSouthern ᵸӚ࠼᷆ȽNorthern ᡌ Western ࠼᷆㔉 ᷒ȽⴤⲺะഖӝ⢟㺞䗴㔉᷒ε

˅㘬ᒿࡇⲴ PCR ࠶᷀㔃᷌˄CBIחᒿࡇ৺ަ⴨䛫Ⲵสഐ㓴ޕᨂ 3-1

˅㘬ᒿࡇ˄CBIחᒿࡇ৺สഐ㓴ޕᨂ 3-2

㘬⡷⇥઼䞦࠷ս⛩㓯ᙗ⽪᜿മחǃสഐ㓴⇥⡷ޕᨂ 3-3

3-4 ᨂޕᮠ઼ᤧ䍍ᮠⲴ Southern ᵲӔ࠶᷀㔃᷌

3-5 MON 87427 ѝнਜ਼䍘㋂僘ᷦᒿࡇⲴ Southern ᵲӔ傼䇱

3-6 ᨂޕᒿࡇцԓっᇊᙗⲴ Southern ᵲӔ࠶᷀

3-7 ᨂޕ⡷⇥㺘䗮ӗ⢙Ⲵ㺘䗮䟿࠶᷀

3-8 ᨂޕ⡷⇥㺘䗮ӗ⢙൘ཊњцԓѝⲴ㺘䗮っᇊᙗ࠶᷀

146 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅㘬ᒿࡇⲴ PCR ࠶᷀㔃᷌˄CBIחᒿࡇ৺ަ⴨䛫Ⲵสഐ㓴ޕᨂ 3-1 ࡙⭘ PCR ᯩ⌅ᢙ໎Ҷ 3 њ⴨ӂ䟽ਐⲴ DNA ⡷⇥˄䱴മ 4ˈӗ⢙ Aǃӗ⢙ B 㘬สഐ㓴ᒿࡇǄPCR ᢙ໎ᡰחᒿࡇ৺ަޕ䮯ᨂޘӗ⢙ C˅ˈ䘉 3 њ⡷⇥⏥ⴆҶ઼ ⭘ᕅ⢙઼ᶑԦ㿱䱴㺘 2Ǆሶ䘉 3 њᢙ໎ӗ⢙䘋㹼ᒿࡇ࠶᷀ᒦ᤬᧕ˈণਟ⺞ᇊޘ䮯 ޕ㘬ᒿࡇ˄䱴മ 5˅Ǆṩᦞᒿࡇ࠶᷀㔃᷌ˈMON 87427 Ⲵᨂחᒿࡇ৺สഐ㓴ޕᨂ ᒿࡇབྷሿѪ 3746 bpˈަѝ T-DNA ᨂޕѪ 3681 bpǄਖཆ൘ T-DNA Ⲵ 5'ㄟ઼ 3' ㄟ࠶࡛ᴹ 41 bp ઼ 24 bp Ⲵᨂޕˈ䘉ᱟ൘ T-DNA ᨂޕ䗷〻ѝ㠚㹼ᨂޕⲴᒿࡇǄ䘉䗷〻ѝᑨ㿱ˈਟ㜭ᱟ⭡Ҿৼ䬮ᯝ㻲؞༽ᵪࡦᕅ䎧ޕ⿽⧠䊑൘ཆⓀ DNA ᨂ ˄Salomon and Puchta, 1998˅Ǆ ↔ཆˈᒿࡇ࠶᷀ҏ䇱ᇎ T-DNA ޵ cp4 epsps สഐ㺘䗮ⴂᱟᆼᮤⲴˈަѝ਴䚇 ՐݳԦⲴᧂࡇᯩᔿоަ൘ PV-ZMAP1043 䖜ॆ䍘㋂кⲴᧂࡇᯩᔿа㠤Ǆ ᒿࡇޕ㘬ᒿࡇԕ৺㔃ᶴ㿱䱴മ 5 ઼䱴മ 6˗ᨂחᒿࡇ઼สഐ㓴ޕMON 87427 Ⲵᨂ ѝⲴ䈳᧗ݳԦ᧿䘠㿱䱴㺘 3Ǆ

147 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅ᒿࡇPCRᢙ໎࠶᷀˄CBIחᒿࡇ৺สഐ㓴ᯱޕ䮯ᨂޘ䱴㺘2. MON 87427

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

148 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᒿࡇѝⲴ䚇Ր䈳᧗ݳԦ৺ަᆳᒿࡇ≷ᙫޕ䱴㺘3. MON 87427ᨂ

˅⥞䚇ՐݳԦ བྷሿ˄ս㖞*˅ ࣏㜭᧿䘠˄৲㘳᮷ 1003 bp ս⛩ 5'ㄟ⴨䛫Ⲵ⦹㊣สഐ㓴 DNAޕ㘬ᒿࡇ ᨂחㄟ'5 ˄1-1003˅ 252 bp ṩⱼߌᵶ㧼 T-DNA ᐖ䗩⭼ᒿࡇˈ৲о T-DNA T-DNA ᐖ䗩⭼ᒿࡇ ˄1004-1255˅ 䖜઼〫ᮤਸ˄Barker et al., 1983˅ 41 bp 䰤ᨂᒿࡇ DNA ݻ䲶઼䖭փᶴᔪ䴰㾱 ˄1256-1296˅ वਜ਼ᴹ䟽༽໎ᕪ४ฏ˄Kay et al., 1987˅Ⲵ㣡 621 bp e35S ੟ࣘᆀ ὠ㨌㣡ਦ⯵∂˄CaMV˅35S RNA Ⲵ੟ࣘᆀ ˄1297-1917˅ ˄Odell et al., 1985˅ 21 bp 䰤ᨂᒿࡇ DNA ݻ䲶઼ᶴᔪ䴰㾱 ˄1918-1938˅ 804 bp ⦹㊣✝◰㳻ⲭ 70 สഐⲴㅜањ޵ਜ਼ᆀ˄Brown hsp70 ޵ਜ਼ᆀ ˄1939-2742˅ and Santino, 1997˅ 24 bp 䰤ᨂᒿࡇ DNA ݻ䲶઼䖭փᶴᔪ䴰㾱 ˄2743-2766˅ 㕆⸱ᤏই㣕 EPSPS 䞦ਦ㔯փ䖜〫㛭४ฏⲴส 228 bp CTP2 ਦ㔯փ䖜〫㛭 ഐ ShkG Ⲵ䶦ḷᒿࡇ˄Herrmann, 1995; Klee et ˄2767-2994˅ al., 1987˅ˈᕅሬ CP4 EPSPS 䖜〫ࡠਦ㔯փ 㓿ᇶ⸱ᆀՈॆⲴṩⱼߌᵶ㧼 CP4 㧼Ṛ aroA ส 1368 bp cp4 epsps 㕆⸱ᒿࡇ ഐ㕆⸱ᒿࡇˈ㕆⸱ CP4 EPSPS 㳻ⲭ˄Barry et ˄2995-4362˅ al., 1992; Padgette et al., 1996˅ 6bp 䰤ᨂᒿࡇ DNA ݻ䲶઼䖭փᶴᔪ䴰㾱 ˄4363-4368˅ ṩⱼߌᵶ㧼㜝㜲⻡ਸ䞦สഐ˄nos˅3'ㄟ䶎䖜ᖅ 253 bp nos 㓸→ᆀ ४ˈ㓸→䖜ᖅᒦфᕅሬ mRNA Ⲵཊ㚊㞪㤧䞨 ˄4369-4621˅ ॆ˄Bevan et al., 1983˅ 27 bp 䰤ᨂᒿࡇ DNA ݻ䲶઼䖭փᶴᔪ䴰㾱 ˄4622-4648˅ ṩⱼߌᵶ㧼 T-DNA ਣ䗩⭼ᒿࡇˈ৲о T-DNA 36 bp T-DNA ਣ䗩⭼ᒿࡇ 䖜઼〫ᮤਸ˄Depicker et al., 1982; Zambryski ˄4649-4684˅ et al., 1982˅ 1092 bp ս⛩ 3'ㄟ⴨䛫Ⲵ⦹㊣สഐ㓴 DNAޕ㘬ᒿࡇ ᨂחㄟ'3 ˄4685-5776˅

㘬ᒿࡇ˄㿱䱴മ 5˅Ⲵ⴨ᓄս㖞Ǆחᒿࡇ઼ޕᤜਧḷࠪⲴս㖞ሩᓄҾ MON 87427 ᨂ *

149 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

˅㘬สഐ㓴⡷⇥Ⲵ䟽ਐPCR࠶᷀˄CBIח४৺ޕ䱴മ4. ⏥ⴆMON 87427ᨂ ᢙ໎⡷⇥Ⲵ亴ᵏབྷሿᱮ⽪൘ PCR ӗ⢙ࠍ㜦മлᯩⲴ MON 87427 ᨂޕᒿࡇ㓯 ᖒമкǄ⇿⌣䚃кṧ䟿Ѫ 4~9 lPCRᢙ໎ӗ⢙Ǆ⌣䚃䈤᰾ྲл˖

㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠

150 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅㘬ᒿࡇ˄CBIחᒿࡇ৺สഐ㓴ޕᨂ 3-2

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

˅㘬⦹㊣สഐ㓴ᒿࡇ˄CBIחᒿࡇ઼ޕ䮯ᨂޘ 䱴മ 5. MON 87427

151 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㘬⡷⇥઼䞦࠷ս⛩㓯ᙗ⽪᜿മחǃสഐ㓴⇥⡷ޕᨂ 3-3 䱴㺘4. MON 87427ⲴSouthernᵲӔѝ䲀ࡦᙗ޵࠷䞦ǃᵲӔ᧒䪸઼亴ᵏ⡷⇥≷ᙫ

Southern ᵲӔ㔃᷌ 䱴മ 7 䱴മ 8 䱴മ 9 䱴മ 10 ⭘Ⲵ᧒䪸 ᧒䪸 1 ઼ 4 ᧒䪸 2 ᧒䪸 3 ᧒䪸 5ǃ6 ઼ 7֯ Ự⍻ⴞⲴ ᨂޕ/ᤧ䍍ᮠ ᨂޕ/ᤧ䍍ᮠ ᨂޕ/ᤧ䍍ᮠ 䍘㋂僘ᷦᒿࡇ

*㺘⽪ᵚ⭘⴨ᓄ䞦䘋㹼䞦࠷࠶᷀

152 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427

Nsi I 2533 Nsi I 4490 Nco I4931 Nsi I 795 Nco I2766 r1 r1

1 nos - e35S - T

hsp70 CTP2 5776 - - P I TS cp4 epsps - CS Left Border Region - B B-Right Border Region

Probe 1 Probe 2

Probe 3

Probe 4 ~ 2.2 kb Nco I

~ 1.7 kb ~ 2.0 kb Nsi I

㘬ᒿࡇ৺亴ᵏ䞦࠷⡷⇥བྷሿⲴ㓯ᙗ⽪᜿മחᒿࡇǃสഐ㓴ޕ䱴മ6. MON 87427ᨂ ᒿࡇлᯩ㇝ཤ㺘⽪䖜ᖅᯩੁˈⴤ䀂㇝ཤ㺘᰾ޕᒿࡇ䚇Ր㓴ᡀݳԦ઼ Southern ᵲӔ࠶᷀ѝⲴ䲀ࡦᙗ䞦࠷ս⛩ˈᨂޕമк䜘㺘⽪ᨂ 㘬ᒿࡇⲴᔰ࿻ս㖞઼ᯩੁǄമл䜘ᡰ⽪Ѫ䲀ࡦᙗ䞦Ⲵ亴ᵏ䞦࠷⡷⇥བྷሿˈᵜ⽪᜿മᒦᵚ᤹∄ֻ㔈ࡦˈഐ↔䚇ՐݳԦⲴח㊣สഐ㓴⦹ ս㖞઼ T-DNA ᧒䪸བྷሿѪ䘁լ٬Ǆ

153 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3-4 ᨂޕᮠ઼ᤧ䍍ᮠⲴ Southern ᵲӔ࠶᷀㔃᷌ ⭘䲀ࡦᙗ޵࠷䞦 Nco I ᡆ Nsi I ⎸ॆ MON 87427 สഐ㓴 DNAˈԕ㜭ཏ⏥ⴆ T-DNA ᒿࡇޘ䮯Ⲵ⴨ӂ䟽ਐ᧒䪸˄᧒䪸 1~᧒䪸 4ˈ䱴മ ઼䱴മ 6˅䘋㹼 Southern ᵲӔ࠶᷀ˈӾ㘼⺞ᇊ MON 87427 ѝⲴᨂޕս⛩ᮠ઼ᨂޕᤧ䍍ᮠǄ 㘬ᒿࡇⲴ㓯ᙗ⢙⨶മ䉡˄䱴മ 6˅ˈ⭘䲀ࡦᙗ޵࠷䞦חᒿࡇ৺ަޕṩᦞᨂ 㘬ᒿࡇкᴹ 1חⲴ T-DNA ޵䜘ਚᴹ 1 њ䞦࠷ս⛩ˈᒦ൘⴨䛫Ⲵ 3'ㄟޕNco I ൘ᨂ њ䞦࠷ս⛩Ǆྲ᷌ T-DNA ൘ MON 87427 ѝѪঅս⛩অᤧ䍍ᨂޕˈࡉ亴ᵏ⭘ Nco I 䞦⎸ॆ MON 87427 สഐ㓴 DNA ਾ㜭ཏӗ⭏ 2 њ䞦࠷⡷⇥ˈަѝањ⡷⇥བྷሿ ˈ㘬ᒿࡇח亴ᵏѪ~2.2 kbˈवਜ਼ CTP2ǃcp4 epspsǃnosǃਣ䗩⭼ᒿࡇ઼ 3'ㄟ䜘࠶ 㘬ᒿࡇǃᐖ䗩⭼ᒿࡇǃח㘼ਖཆањ䗩⭼⡷⇥亴ᵏı2.8 kbˈवਜ਼ᵚ⸕བྷሿⲴ 5'ㄟ e35S ઼ hsp70Ǆ 㘬ᒿח䲀ࡦᙗ޵࠷䞦 Nsi I ൘ T-DNA ޵䜘ᴹ 2 њ䞦࠷ս⛩ˈ൘ަ䛫᧕Ⲵ 5'ㄟ ࡇкᴹ 1 њ䞦࠷ս⛩Ǆྲ᷌ T-DNA ൘ MON 87427 ѝѪঅս⛩অᤧ䍍ᨂޕˈࡉ 亴ᵏ⭘ Nsi I ⎸ॆ MON 87427 สഐ㓴 DNA ਾ㜭ཏӗ⭏ 3 њ䞦࠷⡷⇥Ǆަѝаᶑ ˗㘬ᒿࡇǃᐖ䗩⭼ᒿࡇǃe35S ઼䜘࠶ hsp70חkbˈ䈕ᶑᑖवᤜᐢ⸕བྷሿⲴ 5'ㄟ 1.7~ аᶑᑖ~2.0 kbˈवᤜ䜘࠶ hsp70ǃCTP2ǃcp4 epsps ઼䜘࠶ nos˗ਖཆаᶑᑖı1.3 kbˈ 㘬ᒿࡇǄחवᤜ䜘࠶ nosǃਣ䗩⭼ᒿࡇ઼ᵚ⸕བྷሿⲴ 3'ㄟ MON 87427 ᱟԕ LH198 × HiII ᵲӔ⿽֌Ѫਇփ䖜ॆⲴˈഐ↔䟷⭘ LH198 × HiII ֌Ѫ䱤ᙗሩ➗ˈ਼ᰦ⭘㓿 Sph I 䞦࠷Ⲵ PV-ZMAP1043 䍘㋂ DNA ᡆ ⺞᧒䪸֌Ѫ䱣ᙗሩ➗Ǆ䱣ᙗሩ➗кṧ䟿Ѫ~0.1 ؽ઼~1.0 ؽสഐ㓴 DNA Ⲵ䟿ˈԕ ᇊ Southern ᵲӔⲴ⚥᭿ᓖǄ⭘᧒䪸 1~4˄䱴മ ઼䱴മ 6˅䘋㹼 Southern ᵲӔਟ 㜭Պࠪ⧠ⲴᵲӔᑖර≷ᙫҾ䱴㺘 4ˈᵲӔ㔃᷌㿱䱴മ 7ǃമ 15 ઼䱴മ 9Ǆ 1˅᧒䪸 1 ઼᧒䪸 4 ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆᰦˈ൘~6.1 kb ઼~4.1 kb ༴ӗ⭏޵ⓀᙗᵲӔᶑᑖ˄䱴മ 7ˈ⌣䚃 1 ઼ 8˅˗㘼⭘ Nsi I ⎸ॆᰦˈ൘~9.8 kb ⍻kb ༴ӗ⭏޵ⓀᙗᵲӔᶑᑖ˄䱴മ 7ˈ⌣䚃 3 ઼ 10˅Ǆ䘉ӋᵲӔؑਧ൘ 4.3~઼ ⢩䈅⢙઼ᑨ㿴ሩ➗⌣䚃ѝ൷ᆈ൘ˈഐ↔䇔Ѫ䘉Ӌؑਧᱟ޵ⓀᙗⲴ㛼ᲟᵲӔˈᒦ䶎 DNAǄ䘉Ӌؑਧᖸਟ㜭ᱟ⭡᧒䪸 1о޵Ⓚ Hsp70 ޕᔲᙗൠ䪸ሩMON 87427ѝⲴᨂ ᒿࡇⲴᵲӔᕅ䎧ⲴˈഐѪ䈕᧒䪸ਜ਼ᴹаሿ䜘࠶ Hsp70 ޵ਜ਼ᆀ˄䱴മ 6˅Ǆ⭡Ҿ᧒ 䪸 1 ӵ㾶ⴆҶ Hsp70 ޵ਜ਼ᆀѝᖸሿа䜘࠶ᒿࡇˈᵲӔؑਧ⴨ሩ䖳ᕡǄ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо亴ݸ⭘ Sph I ⎸ॆⲴ PV-ZMAP1043 䍘㋂ DNA ␧ਸ˄䱴മ 7ˈ⌣䚃 7˅ˈ䲔к䘠޵ⓀᙗᵲӔᶑᑖཆˈ 䘈൘~7.1 kb ઼~1.8 kb ༴ӗ⭏ 2 ᶑ亴ᵏᶑᑖ˄䱴മ 6ǃ䱴㺘 4˅Ǆ䱣ᙗሩ➗ᵲӔؑ ਧⲴỰࠪˈ㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔǄ MON 87427 สഐ㓴 DNA ԕ Nco I ⎸ॆ˄䱴മ 7ˈ⌣䚃 2 ઼ 9˅ˈ䲔к䘠޵ ⓀᙗᵲӔᶑᑖཆˈ䘈൘~5.5 kb ઼~2.2 kb ༴ӗ⭏ 2 ᶑᑖǄަѝˈ~5.5 kb ᶑᑖԓ㺘㘬สഐ㓴 DNAˈㅖਸı2.8 kb Ⲵ亴ᵏ䗩⭼⡷⇥བྷሿ˄䱴חT-DNA ৺ަ䛫᧕ ޕҶᨂ 㘬สחDNA Ⲵ 3'ᵛㄟ৺ަ 3'ㄟ䛫᧕Ⲵ ޕമ 6ǃ䱴㺘 4˅˗~2.2 kb ᶑᑖԓ㺘Ҷᨂ 䱴മ 6ǃ䱴㺘 4˅Ǆ˄ഐ㓴 DNAˈ䈕㔃᷌ㅖਸ~2.2 kb 䗩⭼⡷⇥Ⲵ亴ᵏ٬

154 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 MON 87427 สഐ㓴 DNA ԕ Nsi I ⎸ॆ˄䱴മ 7ˈ⌣䚃 4 ઼ 11˅ˈ䲔к䘠޵ ⓀᙗᵲӔᶑᑖཆˈ䘈൘~6.4 kbǃ~2.0 kb ઼~1.7 kb ༴ӗ⭏ 3 ᶑᑖǄަѝˈ~1.7 kb 㘬สഐ㓴 DNAˈㅖ ਸ ~1.7 kbחⲴ T-DNA 5'ㄟ৺ަ䛫᧕Ⲵаሿ䜘࠶ޕԓ㺘ᨂ⇥⡷ DNA ޵䜘Ⲵа䜘 ޕ䱴മ 6ǃ䱴㺘 4˅˗~2.0 kb ᶑᑖԓ㺘ᨂ˄Ⲵ䗩⭼⡷⇥亴ᵏ٬ ࠶ᒿࡇˈㅖਸ~2.0 kb Ⲵ亴ᵏ⡷⇥བྷሿ˄䱴മ 6ǃ䱴㺘 4˅˗~6.4 kb ᶑᑖԓ㺘ᨂޕ 㘬สഐ㓴 DNAˈㅖਸı1.3 kb 䗩⭼⡷⇥Ⲵ亴ᵏབྷח'T-DNA Ⲵ 3'ㄟ৺ަ䛫᧕Ⲵ 3 ሿ˄䱴മ 6ǃ䱴㺘 4˅Ǆ 䱴മ 7 ᡰ⽪㔃᷌㺘᰾ˈ᧒䪸 1 ઼᧒䪸 4 ᡰ⏥ⴆⲴᒿࡇӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ MON 87427 ⲴঅњਟỰ⍻สഐᓗѝǄ 2˅᧒䪸 2 ᑨ㿴ሩ➗ LH198 × HiII ઼ MON 87427 Ⲵสഐ㓴 DNA ࠶࡛⭘ Nco I ᡆ Nsi I ⢙മ 15˅ˈ൷ӗ⭏ҶаӋ޵ⓀᙗᵲӔؑਧˈ䘉ӋᵲӔؑਧ൷ᆈ൘Ҿ⍻䈅˄ॆ⎸ ᑨ㿴ሩ➗⌣䚃ѝ˄മ 15ˈ⌣䚃 1~11˅Ǆഐ↔䇔Ѫ䘉Ӌؑਧᱟ޵ⓀᙗⲴ㛼Ჟᵲ઼ DNAǄ䘉Ӌؑਧᖸਟ㜭ᱟ⭡᧒䪸 2 ޕӔˈᒦ䶎⢩ᔲᙗൠ䪸ሩ MON 87427 ѝⲴᨂ о޵Ⓚ hsp70 ᒿࡇⲴᵲӔᕅ䎧ⲴˈഐѪ䈕᧒䪸ਜ਼ᴹᖸབྷа䜘࠶ hsp70 ޵ਜ਼ᆀᒿࡇ ˄䱴മ ǃ䱴മ 6˅Ǆ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆ˄മ 15ˈ⌣䚃 1 ઼ 8˅ˈ ൘~4.1 kb ༴ӗ⭏޵ⓀᙗᵲӔᶑᑖ˗ԕ Nsi I ⎸ॆ˄മ 15ˈ⌣䚃 3 ઼ 10˅ˈ൘~5.2 kb ઼~4.2 kb ༴ӗ⭏޵ⓀᙗᵲӔᶑᑖǄ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘㋂ӗ⭏Ⲵ᧒䪸⁑ᶯ␧ਸ˄䱴മ ˈ᧒䪸 2˅ˈ䲔к䘠޵ⓀᙗᵲӔᶑᑖཆˈ䘈൘~1.0 kb ༴ӗ⭏亴ᵏᶑᑖ˄മ 15ˈ⌣䚃 5 ઼ 6˅Ǆᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо亴ݸ⭘ Sph I ⎸ॆⲴ PV-ZMAP1043 䍘㋂ DNA ␧ਸ˄മ 15ˈ⌣䚃䲔к䘠޵ⓀᙗᵲӔᶑᑖཆˈ䘈൘~7.1 kb ༴ӗ⭏亴ᵏᶑᑖǄ䱣ᙗሩ➗ᵲӔؑˈ˅7 ਧⲴỰࠪ㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔǄ MON 87427 สഐ㓴 DNA ԕ Nco I ⎸ॆ˄മ 15ˈ⌣䚃 2 ઼ 9˅ˈ䲔к䘠޵Ⓚ ᙗᵲӔᶑᑖཆˈ䘈൘~5.5 kb ઼~2.2 kb ༴ӗ⭏ 2 ᶑᑖǄަѝˈ~5.5 kb ᶑᑖԓ㺘Ҷ 㘬สഐ㓴 DNAˈㅖਸı2.8 kb Ⲵ亴ᵏ䗩⭼⡷⇥བྷሿ˄䱴חT-DNA ৺ަ䛫᧕ ޕᨂ 㘬สחDNA Ⲵ 3'ᵛㄟ৺ަ 3'ㄟ䛫᧕Ⲵ ޕമ 6ǃ䱴㺘 4˅˗~2.2 kb ᶑᑖԓ㺘Ҷᨂ 䱴മ 6ǃ䱴㺘 4˅Ǆ˄ഐ㓴 DNAˈ䈕㔃᷌ㅖਸ~2.2 kb 䗩⭼⡷⇥Ⲵ亴ᵏ٬ MON 87427 สഐ㓴 DNA ԕ Nsi I ⎸ॆ˄മ 15ˈ⌣䚃 4 ઼ 11˅ˈ䲔к䘠޵Ⓚ ᙗᵲӔᶑᑖཆˈ䘈൘~2.0 kb ઼~1.7 kb ༴ӗ⭏ 2 ᶑᑖǄަѝˈ~1.7 kb ⡷⇥ᨂޕ ⇥⡷㘬สഐ㓴 DNAˈㅖਸ~1.7 kb Ⲵ䗩⭼חT-DNA Ⲵ 5'ᵛㄟ઼аሿ䜘࠶䛫᧕Ⲵ- DNA Ⲵ޵䜘ᒿࡇˈㅖਸ~2.0 kb ޕ䱴മ 6ǃ䱴㺘 4˅˗~2.0 kb ᶑᑖਜ਼ᴹᨂ˄亴ᵏ٬ Ⲵ亴ᵏ⡷⇥བྷሿ˄䱴മ 6ǃ䱴㺘 4˅Ǆ മ 15 ᡰ⽪㔃᷌㺘᰾ˈ᧒䪸 2 ᡰ⏥ⴆⲴᒿࡇӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ MON 87427 ⲴঅњਟỰ⍻สഐᓗѝǄ 3˅᧒䪸 3

155 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᑨ㿴ሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA ⭘ Nco ˄䱴മI 9ˈ⌣䚃 1 ઼ 8˅ᡆ Nsi I ˄䱴മ 9ˈ⌣䚃 3 ઼ 10˅࠶࡛⎸ॆˈྲ亴ᵏᡰᯉˈ䈕䱤ᙗሩ➗⋑ᴹӗ⭏ਟỰ⍻Ⲵ ᵲӔᶑᑖǄ ᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘㋂ӗ⭏Ⲵ᧒䪸⁑ᶯ␧ਸ˄䱴മˈ᧒䪸 3˅ˈ൘ ~1.5 kb ༴ӗ⭏亴ᵏᶑᑖ˄䱴മ 9ˈ 䚃 5 ઼ 6˅Ǆᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо亴ݸ⭘ Sph I⌣ ⎸ॆⲴ PV-ZMAP1043 䍘㋂ DNA ␧ਸ˄䱴മ 9ˈ⌣䚃 7˅ˈ൘~1.8 kb ༴ӗ⭏亴 ᵏᶑᑖ˄䱴മ 6ǃ䱴㺘 4˅Ǆᶕ㠚Ҿ䍘㋂Ⲵਖа Sph I ⡷⇥˄~7.1 kb˅ਜ਼ᴹ᧒䪸 3 ᒿࡇⲴаሿ䜘࠶ˈн䗷൘ᵜ⹄ウѝⲴᵲӔ৽ᓄᶑᑖлᵚ㜭ӗ⭏ਟỰ⍻ⲴᵲӔؑਧǄ 䱣ᙗሩ➗ᵲӔؑਧⲴỰࠪ㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔǄ MON 87427 สഐ㓴 DNA ԕ Nco I ⎸ॆ˄䱴മ 9ˈ⌣䚃 2 ઼ 9˅ˈ൘~2.2 kb 㘬สחDNA Ⲵ 3'ᵛㄟ৺ަ 3'ㄟ䛫᧕Ⲵ ޕ༴ӗ⭏ 1 ᶑᑖǄ䈕~2.2 kb ᶑᑖԓ㺘Ҷᨂ 䱴മ 6ǃ䱴㺘 4˅ǄMON 87427˄ഐ㓴 DNA˗䈕㔃᷌ㅖਸ~2.2 kb 䗩⭼⡷⇥Ⲵ亴ᵏ٬ สഐ㓴 DNA ԕ Nsi I ⎸ॆ˄䱴മ 9ˈ⌣䚃 4 ઼ 11˅ˈ൘~2.0 kb ༴ӗ⭏ 1 ᶑᑖˈ ↓ྲ亴ᵏⲴаṧ˄䱴മ 6ǃ䱴㺘 4˅Ǆ䈕 ~2.0 kb ᶑᑖਜ਼ᴹᨂޕ DNA Ⲵ޵䜘ᒿࡇǄ 䱴മ 9 ᡰ⽪㔃᷌㺘᰾ˈ᧒䪸 3 ᡰ⏥ⴆⲴᒿࡇӵԕঅᤧ䍍ᖒᔿᆈ൘Ҿ MON 87427 ⲴঅњਟỰ⍻สഐᓗѝǄ

156 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴മ7. MON 87427ѝT-DNAᨂޕս⛩઼ᤧ䍍ᮠⲴSouthernঠ䘩˄᧒䪸1઼᧒䪸4˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢Ǆ᧒䪸 1 ઼᧒䪸 4 ӵ㾶ⴆ䜘࠶ T-DNA˄䱴 മ઼മ13˅Ǆ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ޵࠷䞦⎸ॆⲴ DNA ṧ૱Ǆ⌣䚃䈤᰾ྲ л˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ [᧒䪸 1 ઼᧒䪸 4 [~0.1 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 5 [᧒䪸 1 ઼᧒䪸 4 [~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 6 [䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 7 8 ሩ➗ LH198 × HiII˄Nco I˅ 9 MON 87427˄Nco I˅ 10 ሩ➗ LH198 × HiII˄Nsi I˅ 11 MON 87427˄Nsi I˅

㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠

157 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴മ8. MON 87427ѝT-DNAᨂޕս⛩઼ᤧ䍍ᮠⲴSouthernঠ䘩˄᧒䪸2˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢Ǆ᧒䪸 2 ӵ㾶ⴆ䜘࠶ T-DNA˄മ 6 ઼മ 13˅Ǆ ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ޵࠷䞦⎸ॆⲴ DNA ṧ૱Ǆ⌣䚃䈤᰾ྲл˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ [᧒䪸 2 [~0.1 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 5 [᧒䪸 2 [~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 6 [䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 7 8 ሩ➗ LH198 × HiII˄Nco I˅ 9 MON 87427˄Nco I˅ 10 ሩ➗ LH198 × HiII˄Nsi I˅ 11 MON 87427˄Nsi I˅

㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠

158 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴മ9. MON 87427ѝT-DNAᨂޕս⛩઼ᤧ䍍ᮠⲴSouthernঠ䘩˄᧒䪸3˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢Ǆ᧒䪸 3 ӵ㾶ⴆ䜘࠶ T-DNA˄䱴മ ઼മ 13˅Ǆ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ޵࠷䞦⎸ॆⲴ DNA ṧ૱Ǆ⌣䚃䈤᰾ྲл˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ [᧒䪸 3 [~0.1 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 5 [᧒䪸 3 [~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 6 [䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿ޕሩ➗ LH198 × HiII˄Nco I˅␧ 7 8 ሩ➗ LH198 × HiII˄Nco I˅ 9 MON 87427˄Nco I˅ 10 ሩ➗ LH198 × HiII˄Nsi I˅ 11 MON 87427˄Nsi I˅

㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠

159 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3-5 MON 87427 ѝнਜ਼䍘㋂僘ᷦᒿࡇⲴ Southern ᵲӔ傼䇱 ൘ MON 87427 ᨂޕᒿࡇѝнवਜ਼ PV-ZMAP1043 䍘㋂Ⲵԫօ僘ᷦᒿࡇǄ਼ ṧ⭘ Nco I ᡆ Nsi I 䲀ࡦᙗ޵࠷䞦⎸ॆ MON 87427 สഐ㓴 DNAˈᒦ⭘᧒䪸 5~7 䘋㹼ᵲӔਟԕ⺞ᇊ MON 87427 ᱟ੖ਜ਼ᴹ僘ᷦᒿࡇǄ᧒䪸 5~7 ਟԕ㾶ⴆ PV-ZMAP1043 䍘㋂Ⲵޘ䜘僘ᷦᒿࡇ˄䱴മ ˅Ǆ⭘᧒䪸 5~7 䘋㹼 Southern ᵲӔ ਟ㜭Պࠪ⧠ⲴᵲӔᑖර≷ᙫҾ䱴㺘 4ǄSouthern ᵲӔ㔃᷌㿱䱴മ 10Ǆ ᑨ㿴ሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA ⭘ Nco I˄䱴മ 10ˈ⌣䚃 1 ઼ 10˅ᡆ Nsi I˄䱴മ 10ˈ⌣䚃 3 ઼ 12˅࠶࡛⎸ॆˈ⭘ਟԕ㾶ⴆ PV-ZMAP1043 䍘㋂僘ᷦ ᒿࡇⲴ᧒䪸 5ǃ᧒䪸 6 ઼᧒䪸 7 ਼ᰦᵲӔˈ↓ྲ䱤ᙗሩ➗亴ᵏᓄᴹ㔃᷌аṧˈ⋑ ᴹӗ⭏ਟỰ⍻ⲴᵲӔᶑᑖǄᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾ о㓿 Sph I ⎸ॆⲴ PV-ZMAP1043 䍘㋂␧ਸˈ൘~7.1 kb ༴ӗ⭏亴ᵏᶑᑖ˄䱴മ 10ˈ ⌣䚃 9˗䱴㺘 4˅Ǆᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘㋂ӗ⭏Ⲵ᧒䪸 5 ઼᧒䪸 6˄䱴മ ˅Ⲵ⁑ᶯ␧ਸˈ൘~1.8 kb ઼ ~1.5 kb ༴ӗ⭏ 2 ᶑ亴ᵏᶑᑖ˄䱴മ 10ˈ⌣䚃 5 ઼ 6˗䱴㺘 4˅Ǆᑨ㿴ሩ➗ LH198 × HiII สഐ㓴 DNA ԕ Nco I ⎸ॆਾо⭡ PV-ZMAP1043 䍘㋂ӗ⭏Ⲵ᧒䪸 7˄䱴മ ˅Ⲵ ⁑ᶯ␧ਸˈ൘~1.7 kb ༴ӗ⭏亴ᵏᶑᑖ˄䱴മ 10ˈ⌣䚃 7 ઼ 8˗䱴㺘 4˅Ǆ䘉Ӌ䱣 ᙗሩ➗ᵲӔؑਧⲴỰࠪ㔃᷌㺘᰾᧒䪸оަ䶦ḷᒿࡇਁ⭏ҶᵲӔǄ ᑨ㿴ሩ➗ LH198 × HiII Ⲵสഐ㓴 DNA ⭘ Nco I˄䱴മ 10ˈ⌣䚃 2 ઼ 11˅ᡆ Nsi I˄䱴മ 10ˈ⌣䚃 4 ઼ 13˅࠶࡛⎸ॆˈ⭘ਟԕ㾶ⴆ PV-ZMAP1043 䍘㋂僘ᷦ ᒿࡇⲴ᧒䪸 5ǃ᧒䪸 6 ઼᧒䪸 7 ਼ᰦᵲӔˈ↓ྲ亴ᵏᓄᴹ㔃᷌аṧ˄䱴㺘 4˅ˈ ⋑ᴹԫօᵲӔᶑᑖࠪ⧠Ǆ䘉а㔃᷌㺘᰾ˈMON 87427 нਜ਼ᴹԫօ PV-ZMAP1043 䍘㋂Ⲵ僘ᷦᒿࡇǄ

160 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴മ10. MON 8742нਜ਼䍘㋂僘ᷦᒿࡇⲴSouthernᵲӔ࠶᷀˄᧒䪸5~7˅ ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢Ǆ᧒䪸 5ǃ᧒䪸 6 ઼᧒䪸 7 ਟԕ㾶ⴆޘ䜘 PV-ZMAP1043 䍘㋂Ⲵ僘ᷦᒿࡇ˄䱴മ ˅Ǆ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ޵࠷䞦⎸ ॆⲴ DNA ṧ૱Ǆ⌣䚃䈤᰾ྲл˖ ⌣䚃 ṧ૱䈤᰾ 1 ሩ➗ LH198 × HiII˄Nco I˅ 2 MON 87427˄Nco I˅ 3 ሩ➗ LH198 × HiII˄Nsi I˅ 4 MON 87427˄Nsi I˅ ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 5 ઼᧒䪸 6[~0.1ؽสഐ㓴ᖃ䟿]␧ਸ 5 ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 5 ઼᧒䪸 6[~1.0ؽสഐ㓴ᖃ䟿]␧ਸ 6 ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 7 [~0.1 ؽสഐ㓴ᖃ䟿]␧ਸ 7 ሩ➗ LH198 × HiII˄Nco I˅о᧒䪸 7 [~1.0 ؽสഐ㓴ᖃ䟿]␧ਸ 8 ሩ➗ LH198 × HiII˄Nco I˅䞦࠷䍘㋂˄Sph I˅[~1.0 ؽสഐ㓴ᖃ䟿]␧ਸ 9 10 ሩ➗ LH198 × HiII˄Nco I˅ 11 MON 87427˄Nco I˅ 12 ሩ➗ LH198 × HiII˄Nsi I˅ 13 MON 87427˄Nsi I˅ ㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠 3-6 ᨂޕᒿࡇцԓっᇊᙗⲴ Southern ᵲӔ࠶᷀

161 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 1˅н਼цԓ䰤 T-DNA っᇊᙗⲴ Southern ᵲӔ࠶᷀ ޡ䘋㹼Ҷ 5 њцԓᶀᯉ T-DNA ᨂޕっᇊᙗⲴ Southern ᵲӔ࠶᷀Ǆ䘉 5 њц ԓवᤜ LH198 BC3F3 ǃ LH198 BC3F4 ǃ LH198 BC3F6 ǃ LH198 BC3F7 ઼ ˄LH198 BC3F7 × LH287˅F1˄䱴മ 11˅ˈަѝ LH198 BC3F4 ᱟ⭘ᶕ䘋㹼࠶ᆀ ⢩ᖱ⢩ᙗ࠶᷀Ⲵцԓ˄䱴മ 7ǃമ 15 ઼䱴മ 9 ઼䱴മ 10˅Ǆ䖜ॆਇփᵲӔ⿽ LH198 × HiII ԕ৺ᵲӔ⿽ LH198 × LH287 ֌Ѫ Southern ᵲӔⲴሩ➗Ǆ䴰㾱ᤷࠪ Ⲵᱟˈቭ㇑㠚 R0 ԓᔰ࿻䖜ॆἽṚᔰ࿻⭘ LH198 䘋㹼ཊԓഎӔˈަ䚇Ր㛼Ჟสᵜ Ҿ LH198ˈն׍❦ਟԕ㛟ᇊަ䚇Ր㛼Ჟѝᴹਟ㜭ਜ਼ᴹ HiII˄∄ྲ൘ BC3F4ੁٮ цԓਜ਼ᴹ 94%Ⲵ LH198 䚇Ր㛼Ჟ઼ 6%Ⲵ HiII 䚇Ր㛼Ჟ˅Ǆ㘳㲁ࡠਟ㜭ᆈ൘Ⲵ޵ Ⓚ㛼Ჟˈ䘹⭘ਇփ LH198 × HiII˄㘼䶎 LH198˅֌Ѫሩ➗Ǆ Ӿ MON 87427 Ⲵн਼цԓᶀᯉԕ৺ሩ➗ᶀᯉᨀਆ DNA˄LH198 BC3F3ǃ LH198 BC3F6 ઼ LH198 BC3F7 Ⲵ DNA ᱟӾਦ⡷ᨀਆˈަᆳцԓ DNA ᱟӾ⿽ᆀ ѝᨀਆⲴ˅ˈ㓿䲀ࡦᙗ޵࠷䞦 Nsi I ⎸ॆ˄䱴മ 6˅ˈ⭘ PV-ZMAP1043 䍘㋂Ⲵ᧒䪸 1 ઼᧒䪸 4˄䱴മ ˅䘋㹼 Southern ᵲӔǄྲ᷌ T-DNA ᨂޕ൘цԓ䰤н㜭ཏっᇊ 䚇ՐˈࡉՊ൘ Southern ᵲӔ㔃᷌ѝࠪ⧠䶎亴ᵏᶑᑖ˄亴ᵏᶑᑖ㿱䱴മ 6 ઼䱴㺘 4˅Ǆ⭘᧒䪸 1 ઼᧒䪸 4 ᵲӔⲴ㔃᷌㿱䱴മ 12Ǆ ᰐ䇪ᱟሩ➗䘈ᱟ MON 87427 䜭൘~4.3 kb ༴Ự⍻ࡠаᶑᵲӔᶑᑖ˄䱴മ 12ˈ 䚃 1~10˅ˈഐ↔ਟԕ䇔Ѫ䈕ᵲӔᶑᑖ኎Ҿ⦹㊣޵Ⓚ㛼ᲟⲴᵲӔؑਧˈ㘼о⌣ MON 87427 Ⲵᨂޕ DNA ᰐޣǄ ሩ➗ LH198 × HiII สഐ㓴 DNA ⭘ Nsi I ⎸ॆਾо᧒䪸⁑ᶯ˄᧒䪸 1 ઼᧒䪸 4˅ ␧ਸˈ䲔~4.3 kb Ⲵ޵ⓀᙗᵲӔᶑᑖཆˈ䘈ӗ⭏ҶⓀҾ䍘㋂ PV-ZMAP1043 Ⲵ ~1.2 kb ઼~0.7 kb 亴ᵏབྷሿᶑᑖ˄䱴മ 12ˈ⌣䚃 1 ઼ 2˅Ǆᑨ㿴ሩ➗ LH198 × HiII ˈสഐ㓴 DNA ⭘Nsi I⎸ॆਾо亴ݸ⭘ Sph I⎸ॆⲴ䍘㋂䖭փPV-ZMAP1043 ␧ਸ 䲔~4.3 kb Ⲵ޵ⓀᙗᵲӔᶑᑖཆˈ䘈൘~7.1 kb ઼~1.8 kb ༴ӗ⭏ 2 ᶑ亴ᵏབྷሿⲴᶑ ᑖ˄䱴മ 12ˈ⌣䚃 3˅Ǆ䱣ᙗሩ➗ⲴỰࠪ㺘᰾᧒䪸оަ䶦ḷᒿࡇਟԕᵲӔǄ Ӿ LH198 BC3F3ǃLH198 BC3F4ǃLH198 BC3F6ǃLH198 BC3F7 ઼˄ LH198 BC3F7 × LH287˅F1 цԓᨀਆⲴ DNA ⭘ Nsi I ⎸ॆ˄䱴മ 12ˈ⌣䚃 5ǃ6ǃ7ǃ8 ઼ 10˅ˈ䲔к䘠޵ⓀᙗᵲӔᶑᑖཆˈ䘈൘~6.4 kbǃ~2.0 kb ઼~1.7 kb ༴ӗ⭏ 3 ᶑ 亴ᵏᶑᑖ˄䱴㺘 4˅Ǆ~1.7 kb ᶑᑖԓ㺘Ҷᨂޕ T-DNA Ⲵ 5’ᵛㄟԕ৺аሿ䜘࠶䛫㘬สഐ㓴 DNAˈㅖ ਸ ~1.7 kb 䗩⭼⡷⇥Ⲵ亴ᵏབྷሿ˗~2.0 kb ᶑᑖਜ਼ᴹа䜘ח᧕Ⲵ 㘬䛫חㄟ’3 ޕDNA ޵䜘ᒿࡇǄ~6.4 kb ᶑᑖԓ㺘 T-DNA Ⲵ 3’ᵛㄟԕ৺ᨂ ޕ࠶ᨂ ᧕Ⲵสഐ㓴 DNA ˈㅖਸ@\ 䗩⭼⡷⇥Ⲵ亴ᵏབྷሿǄMON 87427 Ⲵ LH198 BC3F3ǃLH198 BC3F6ǃLH198 BC3F7 ઼˄LH198 BC3F7 × LH287˅F1 цԓ˄䱴മ 12ˈ⌣䚃 5ǃ6ǃ7ǃ8 ઼ 10˅ӗ⭏Ⲵᑖ䉡⢩ᖱо LH198 BC3F4 цԓ ⴨਼˄䱴മ 7ˈ⌣䚃 4 ઼ 11˅ˈ㺘᰾ MON 87427 ѝঅњᤧ䍍Ⲵ T-DNA ൘ᡰ䘹 цԓѝਟԕっᇊ䚇ՐǄ

162 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䘋㹼୶ъॆ ૱⿽䖜㛢

TI: [RP×LH198 BC3F4] BC0F1 ×RP ˅TI: BC1F1 ˄࠶⿫∄ֻ 1:1 ×RP ˅TI: BC2F1 ˄࠶⿫∄ֻ 1:1 ! ˅TI: BC2F2 ˄࠶⿫∄ֻ 3:1

䱴മ11. MON 87427㛢⿽㌫䉡മ Ӿ LH198 BC3F2 ѝ䘹ᤙаṚᣇ㥹⭈㟖ἽṚ䘋а↕㠚Ӕਟԕᗇࡠ LH198 BC3F3ˈ 䘹ᤙ LH198 BC3F3 ѝ㓟ਸփ㠚Ӕᗇࡠ LH198 BC3F4ǄLH198 BC3F4 цԓ⭘Ҿ࠶ᆀ ᖱ⢩ᙗ࠶᷀ˈᒦ⭘֌䘋а↕എӔ䖜㛢ษ㛢ਟ׋୶ъॆᓄ⭘Ⲵ૱⿽ǄLH198 BC3F3ǃ⢩ LH198 BC3F4ǃLH198 BC3F6ǃLH198 BC3F7 ઼[LH198 BC3F7 × LH287] F1 цԓ ˄࣐㋇ᱮ⽪˅㻛⭘Ҿཊцԓ䚇Րっᇊᙗ࠶᷀Ǆ[LH198 BC3F7 × LH287] F1 цԓ⭘ Ҿ䘋㹼㳻ⲭ㺘䗮䟿ǃᡀ࠶ǃ㺘රᙗ⣦ǃߌ㢪ᙗ⣦ԕ৺⧟ຳӂ֌ㅹ࠶᷀Ǆ RP × LH198 BC3F4 ⲴഎӔ઼㠚Ӕਾԓᶀᯉ⭘Ҿᆏᗧቄ䚇Րᆖ࠶᷀Ǆ˄F#Ѫ㠚Ӕц ԓ˗ Ѫ㠚Ӕ˗BC#ѪഎӔцԓǄ˅

163 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴മ 12. MON 87427ᨂޕᒿࡇ൘ཊцԓ䰤っᇊⲴSouthern࠶᷀˖᧒䪸1઼᧒䪸4 ঠ䘩⭘ 32P-ḷ䇠Ⲵ᧒䪸ᵲӔᒦᱮ㢢Ǆ᧒䪸 1 ઼᧒䪸 4 ӵ㾶ⴆ䜘࠶ T-DNA˄䱴 മ઼മ13˅ǄLH198 BC3F3ǃLH198 BC3F6 ઼ LH198 BC3F7 Ⲵ DNA ᱟӾਦ⡷ ᨀਆˈަᆳцԓ DNA ᱟӾ⿽ᆀѝᨀਆⲴǄ⇿⌣䚃кṧ㓖 10 μg 䲀ࡦᙗ޵࠷䞦⎸ ॆⲴ DNA ṧ૱Ǆ⌣䚃䈤᰾ྲл˄ᤜਧ޵㺘⽪⭘Ҿ⎸ॆⲴ޵࠷䞦˅˖ ⌣䚃 ṧ૱᧿䘠 [᧒䪸 1 ઼᧒䪸 4[⴨ᖃҾ~0.1 ؽสഐ㓴 DNAޕሩ➗ LH198 × HiII (Nsi I) ␧ 1 [᧒䪸 1 ઼᧒䪸 4[⴨ᖃҾ~1.0 ؽสഐ㓴 DNAޕሩ➗ LH198 × HiII (Nsi I) ␧ 2 [䞦࠷䍘㋂ (Sph I) [⴨ᖃҾ~1.0 ؽสഐ㓴 DNAޕሩ➗ LH198 × HiII (Nsi I) ␧ 3 4 ሩ➗ LH198 × HiII (Nsi I) 5 MON 87427 (LH198 BC3F3) (Nsi I) 6 MON 87427 (LH198 BC3F4) (Nsi I) 7 MON 87427 (LH198 BC3F6) (Nsi I) 8 MON 87427 (LH198 BC3F7) (Nsi I) 9 ሩ➗ LH198 × LH287 (Nsi I) 10 MON 87427([LH198 BC3F7 × LH287] F1) (Nsi I) ㇝ཤㅖਧᤷ⽪ DNA བྷሿ˄kb˅ˈᶕ㠚ⓤॆ҉䭝ḃ㢢ࠍ㜦к࠶ᆀ䟿ḷ䇠

164 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2˅MON 87427 ሬޕᙗ⣦Ⲵ䚇Ր࠶⿫ ൘ MON 87427 Ⲵ⹄ウᔰਁ䗷〻ѝˈ䘋㹼Ҷ⴨ޣⲴ䚇Ր࠶⿫䈅傼ˈԕ䇴ՠ MON 87427 ѝᨂޕⲴ cp4 epsps สഐ㺘䗮ⴂⲴ䚇ՐっᇊᙗǄ࡙⭘঑ᯩ⍻傼ᶕ∄䖳 ᇎ䱵࠶⿫∄઼ֻ׍ᦞᆏᗧቄ䚇Ր㿴ᖻ䇑㇇Ⲵ亴ᵏ࠶⿫∄ֻⲴᤏਸ〻ᓖǄ ঑ᯩⲴ䇑㇇ޜᔿѪ˖ 02`5|}M

ަѝ˖o= ᇎ䱵㿲ሏࡠⲴสഐරᡆ㺘⧠ර仁⦷˗e= ⨶䇪亴ᵏสഐරᡆ㺘⧠ර仁⦷˗ 㔏䇑ᱮ㪇≤ᒣ䇮ᇊѪ `Ǆ

䖜ॆᗇࡠⲴ R0 ԓἽṚ⭘ LH198 എӔࠐԓਟԕᗇࡠᵲਸⲴ LH198 BC3F1 ц ԓǄሶ LH198 BC3F1 㠚Ӕਟԕᗇࡠ࠶⿫㗔փ LH198 BC3F2ǄӾ LH198 BC3F2 ѝ䘹ᤙаṚᣇ㥹⭈㟖ἽṚ䘋а↕㠚Ӕਟԕᗇࡠ LH198 BC3F3 ˈ䘹ᤙ LH198 BC3F3 ѝ㓟ਸփ㠚Ӕᗇࡠ LH198 BC3F4˄䱴മ 11˅ǄLH198 BC3F4 Ⲵ⿽ ᆀ⭘ҾഎӔ䖜㛢ᶕ䘋а↕ᔰਁ୶ъॆⲴ૱⿽Ǆާփᱟ⭘нਜ਼ cp4 epsps สഐ㺘䗮 ⴂⲴӢᵜ˄〠Ѫ䖞എӢᵜˈRP ˅എӔǄഎӔᖃԓѪᵲਸ㗔փˈ〠Ѫ ˄RP × LH198 BC3F4˅BC0F1Ǆ䘋а↕എӔᗇࡠ˄RP × LH198 BC3F4˅BC1F1ˈ ሩަ௧ᯭ㥹⭈㟖ਟԕ䘹ᤙᣇ㥹⭈㟖ⲴἽṚǄ⭘ᣇᙗἽṚ޽എӔᗇࡠ BC2F1ˈ൘ BC2F1 цԓ䘹ᤙᣇᙗἽṚ㠚Ӕᗇࡠ BC2F2 ԓǄ൘ BC1F1ǃBC2F1 ઼ BC2F2 ц ⨶ԓ䈳ḕ㥹⭈㟖ᣇᙗἽṚⲴ࠶⿫∄ֻˈBC1F1 ઼ BC2F1 ⲴᣇᙗἽṚ:᭿ᝏἽṚⲴ 䇪࠶⿫∄ֻѪ 1:1ˈ㘼 BC2F2 Ⲵ⨶䇪࠶⿫∄ֻѪ 3:1˄䱴മ 11˅Ǆ䈳ḕⲴἽṚᮠǃ 䇪࠶⿫∄ֻ৺ަ঑ᯩ⍻傼㔃᷌㿱䱴㺘 5ǄӾ䘉Ӌ㔃᷌ਟⴻࠪˈMON 87427 Ⲵᣇ⨶ 㥹⭈㟖ᙗ⣦ㅖਸᆏᗧቄঅสഐ䚇Ր㿴ᖻǄӾ㘼䇱ᇎ MON 87427 ѝⲴ cp4 epsps สഐ㺘䗮ⴂ൘অаս⛩кӵᴹঅᤧ䍍ᨂޕˈᒦਟ൘ਾԓѝっᇊ䚇ՐǄ ֻ∄䱴㺘5. MON 87427Ⲵ㓴㓷⢩ᔲᙗ㥹⭈㟖ᣇᙗⲴ䚇Ր࠶⿫

㿲ሏἽṚ 㿲ሏࡠ 㿲ሏࡠ 亴ᵏⲴ 亴ᵏⲴ цԓ 1 2 ᱮ㪇ᙗ ᙫᮠ 2 䱣ᙗṚᮠ 䱤ᙗṚᮠ 䱣ᙗṚᮠ 䱤ᙗṚᮠ

BC1F1 238 109 129 119 119 1.6807 >0.05

BC2F1 290 145 145 145 145 0 >0.05

BC2F2 1107 820 287 830 277 0.5062 >0.05

165 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3-7 ᨂޕ⡷⇥㺘䗮ӗ⢙Ⲵ㺘䗮䟿࠶᷀ 㓴㓷઼ಘᇈѝ㺘䗮Ⲵ CP4 EPSPS 㳻ⲭⲴᇊ䟿ᱟ䙊䗷䞦㚄ݽޣMON 87427 ⴨ ⯛˄ELISA˅Ⲵᯩ⌅ᆼᡀⲴǄMON 87427 Ⲵᶀᯉṧ૱ᱟӾ 2008 ᒤ൘㖾ഭ䘋㹼Ⲵ ⭠䰤䈅傼ѝ䟷䳶Ⲵˈޡ 5 њ䈅傼⛩˄࠶࡛սҾսҾ㖾ഭ⦹㊣ѫӗ४Ⲵ䱯㛟㢢ᐎ Jackcon 䜑ǃ⡡㦧ॾᐎⲴ Jefferson 䜑ǃԺ࡙䈪ԺᐎⲴ Stark 䜑ǃঠㅜᆹ㓣ᐎⲴ Parker 䜑ԕ৺޵ᐳ᣹ᯟ࣐ᐎⲴ York 䜑˅ˈ⇿њ䈅傼⛩Ⲵ䈅傼Ѫ 3 ⅑䟽༽ˈਆṧ൘ᡰᴹ 䈅傼⛩Ⲵᡰᴹ䟽༽䘋㹼Ǆਆṧ઼࠶᷀Ⲵᶀᯉवᤜн਼⭏䮯䱦⇥䟷䳶Ⲵ MON 87427 㓴㓷ṧ૱㊫රޡ䇑 19 ⿽˖ޘ⭏㛢ᵏⲴਦ⡷˄OSL1~4˅ǃ㊭㋂ǃ㣡㊹ǃ㣡эǃൠк䜘ᮤṚǃ㤾ਦǃޘ⭏㛢ᵏṩ㌫˄OSR1~4˅ǃ≄⭏ṩǃ㺠㘱ṩ㌫ ԕ৺ޘ⭏㛢ᵏᮤṚ˄OSWP1~4˅Ǆ ELISA ࠶᷀㔃᷌≷ᙫҾ䱴㺘 6ǄMON 87427 Ⲵн਼㓴㓷઼ಘᇈѝ CP4 EPSPS 940 μg/g dwt 㳻ⲭⲴ㺘䗮䟿ਈॆ㤳ത䖳བྷˈӾᴰሿ٬վҾỰࠪ䲀˄LOD˅ࡠᴰབྷ٬ ᗞݻ/ݻ㓴㓷ᒢ䟽˅Ǆަѝਦ⡷ѝⲴᒣ൷㺘䗮䟿ᴰ儈˄㤳തӾ OSL3 Ⲵ˄ 㤳തӾ OSWP4 Ⲵ˄μg/g dwt ࡠ OSL1 Ⲵ 680 μg/g dwt˅˗ަ⅑ᱟᮤṚᒣ൷٬ 290 μg/g dwt ࡠ OSWP1 Ⲵ 500 μg/g dwt˅Ǆ䲿ਾ׍⅑Ѫṩ㌫˄Ӿ OSR3 Ⲵ 240 73 μg/g dwt ࡠ OSR1 Ⲵ 140 μg/g dwt˅ǃൠк䜘ᮤṚ˄120 μg/g dwt˅ǃ㣡э ˄100 μg/g dwt˅ǃ≄⭏ṩ˄72 μg/g dwt˅ǃ㺠㘱ṩ㌫˄72 μg/g dwt˅ǃ㤾ਦ˄43 μg/g dwt˅ ઼㊭㋂˄4.2 μg/g dwt˅Ǆ ᴹ 6 њ MON 87427 㣡㊹ṧ૱˄ᶕ㠚 3 њ䈅傼⛩˅Ⲵ CP4 EPSPS 㳻ⲭ㺘䗮䟿 վҾỰࠪ䲀˄

166 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴㺘 6. 2008 ᒤ൘㖾ഭ⭠䰤䟷䳶Ⲵ MON 87427 㓴㓷ṧ૱ѝ CP4 EPSPS 㳻ⲭਜ਼䟿 ˅SD˄SD˅ ᒣ൷٬˄ᒣ൷٬ ᫝⿽ਾⲴཙ LOD/LOQ4 㓴㓷㊫ර ਁ㛢ᰦᵏ 1 㤳ത 㤳ത ᮠ˄DAP˅ 2 3 ਦ⡷

OSL1 V2-V5 20-28 100 (21) 680 (170) 0.069/0.137 75 – 140 400 – 940

OSL2 V6-V8 32-46 83 (25) 410 (130) 0.069/0.137 30 –110 130 – 560

OSL3 V10-V12 41-67 61 (19) 290 (74) 0.069/0.137 35 –95 210 – 410

OSL4 VT 54-73 95 (30) 370 (120) 0.069/0.137 17 – 140 70 – 520

㊭㋂ R6 118-182 3.6 (0.73) 4.2 (0.89) 0.16/0.228 2.6 – 5.3 2.8 – 6.2

㣡㊹ 5 ᦸ㊹ᵏ 58-81 < LOD (NA)

㣡э ᦸ㊹ᵏ 58-76 9.4 (0.97) 100 (12) 0.121/0.137 8.1 – 11 90 – 120

ൠк䜘ᮤṚ R5 83-116 38 (14) 120 (48) 0.069/0.137 8.3 – 57 21 – 200

㤾ਦ R6 124-180 14 (6.3) 43 (27) 0.069/0.137 5.9 – 26 13 – 98

ṩ㌫

OSR1 V2-V5 22-28 18 (5.3) 140 (46) 0.033/0.068 8.1 – 27 58 – 210

OSR2 V6-V8 32-46 16 (6.8) 110 (62) 0.033/0.068 8.3 –29 48 – 240

OSR3 V10-V12 41-67 12 (4.3) 73 (28) 0.033/0.068 4.9 – 19 22 – 110

OSR4 VT 54-73 15 (5.7) 83 (36) 0.033/0.068 5.6 – 23 23 – 140

167 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ˅SD˄SD˅ ᒣ൷٬˄ᒣ൷٬ ᫝⿽ਾⲴཙ LOD/LOQ4 㓴㓷㊫ර ਁ㛢ᰦᵏ 1 㤳ത 㤳ത ᮠ˄DAP˅ 2 3

≄⭏ṩ R5 83-116 15 (5.2) 72 (23) 0.033/0.068 8.6 – 24 39 – 100

㺠㘱ṩ㌫ R6 124-180 16 (8.3) 72 (37) 0.033/0.068 5.9 – 29 26 – 130

ᮤṚ

OSWP1 V2-V5 22-28 50 (8.3) 500 (190) 0.069/0.137 37 – 66 310 – 840

OSWP2 V6-V8 32-46 46 (7.6) 360 (42) 0.069/0.137 33 – 58 300 – 420

OSWP3 V10-V12 41-67 43 (7.1) 380 (78) 0.069/0.137 28 – 56 230 – 500

OSWP4 VT 54-73 37 (6.3) 240 (42) 0.069/0.137 23 – 47 160 – 340

1 䟷ṧᰦ⦹㊣ᡰ༴Ⲵਁ㛢ᰦᵏǄ ᴰབྷ-ݻ勌䟽㓴㓷˄fwt˅ѝਜ਼ᴹⲴᗞݻᮠˈᒣ൷٬ǃḷ߶ᐞ˄SD˅઼㤳ത˄ᴰሿ٬⇿ 2 ᱟṩᦞᡰᴹ䈅傼⛩Ⲵṧ૱ᮠᦞ䇑㇇ᶕⲴ˄≄⭏ṩṧ૱ n=11ˈ㣡㊹ṧ૱ n=6ˈަᆳ˅٬ ṧ૱ n=14˅Ǆ ݻᒢ䟽㓴㓷˄dwt˅ѝਜ਼ᴹⲴᗞݻᮠˈᒢ䟽٬ᱟṩᦞ勌䟽٬䲔ԕᒢ䟽䖜ᦒ㌫ᮠ㘼ᗇǄ⇿ 3 4 LOQ=ᇊ䟿䲀ˈLOD=Ựࠪ䲀Ǆ 5 MON 87427 㣡㊹ṧ૱Ⲵ CP4 EPSPS 㳻ⲭ≤ᒣᡆ㘵վҾỰࠪ䲀˄LODˈˈn=6˅ˈ ᡆ㘵ӵ⮕儈Ҿᇊ䟿䲀˄LOQˈˈn=6˅Ǆ

168 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3-8 ᨂޕ⡷⇥㺘䗮ӗ⢙൘ཊњцԓѝⲴ㺘䗮っᇊᙗ࠶᷀ MON 87427 ᱟ䙊䗷ߌᵶ㧼ӻሬ䖜ॆᯩ⌅ሶ PV-ZMAP1043 䍘㋂Ⲵ T-DNA ሬ ޕࡠਇփ⦹㊣ LH198 × HiII ѝ㧧ᗇⲴǄLH198 × HiII ᱟњᵲӔ⿽ˈަѝӢᵜ LH198 ḃ∄䖳᭿ᝏ৸᱃Ҿ࠶ॆ޽⭏ˈᱟᑨ⭘ץᴹ㢟ྭⲴߌ㢪ᙗ⣦ˈ㘼 HiII ሩߌᵶ㧼ާ Ⲵ⦹㊣䖜ॆਇփǄ㧧ᗇⲴ R0 ԓἽṚ䙊䗷оߌ㢪ᙗ⣦䖳ྭⲴ LH198 എӔཊԓˈᒦ 㓿䘎㔝㠚ᵲˈᴰ㓸㧧ᗇ MON 87427ǄMON 87427 Ⲵ䘹㛢䗷〻৺㛢⿽മ䉡㿱䱴മ 11Ǆ ᨂޕᒿࡇ㺘䗮っᇊᙗ࠶᷀वᤜҶ MON 87427 Ⲵ 5 њцԓᶀᯉ˄䱴മ 11˅˖ LH198 BC3F3 цԓⲴਦ⡷઼ LH198 BC3F4ǃLH198 BC3F6ǃLH198 BC3F7 ઼ ˄LH198 BC3F7 × LH287˅F1 цԓⲴ⿽ᆀ˗ሩ➗ᶀᯉѪਇփ LH198 × HiII Ⲵ⿽ ᆀǄ䴰㾱ᤷࠪⲴᱟˈቭ㇑㠚 R0 ԓᔰ࿻䖜ॆἽṚᔰ࿻⭘ LH198 䘋㹼ཊԓഎӔˈަ ∄˄Ҿ LH198ˈն׍❦ਟԕ㛟ᇊަ䚇Ր㛼Ჟѝᴹਟ㜭ਜ਼ᴹ HiIIੁٮ䚇Ր㛼Ჟสᵜ ྲ൘ BC3F4 цԓਜ਼ᴹ 94%Ⲵ LH198 䚇Ր㛼Ჟ઼ 6%Ⲵ HiII 䚇Ր㛼Ჟ˅Ǆ㘳㲁ࡠਟ 㜭ᆈ൘Ⲵ޵Ⓚ㛼Ჟˈ䘹⭘ਇփ LH198 × HiII ֌Ѫሩ➗Ǆབྷ㛐ᵶ㧼㺘䗮Ⲵ CP4 EPSPS 㳻ⲭ˄2ng˅֌Ѫḷ߶৲➗㳻ⲭǄ ࡙⭘ CP EPSPS 㳻ⲭⲴ Western ঠ䘩࠶᷀ᶕ䇱ᇎ MON 87427 ѝᨂޕᒿࡇ㺘䗮 ӗ⢙൘ཊњцԓѝⲴっᇊᙗˈWestern ࠶᷀㔃᷌㿱䱴മ 13Ǆ↓ྲ亴ᵏ㔃᷌аṧˈ MON 87427 Ⲵཊњцԓᶀᯉ൷ਟỰ⍻ࡠ CP4 EPSPS 㳻ⲭᶑᑖ˄䱴മ 13ˈ⌣䚃 ǄLH198 BC3F3 цԓਦ⡷ᶀᯉⲴ Western ݽ⯛ؑਧ˄䱴മ 13ˈ⌣䚃 4˅᰾ᱮ˅8~4 ᕪҾަᆳцԓⲴ⿽ᆀᶀᯉˈ䈤᰾ CP4 EPSPS 㳻ⲭ൘ਦ⡷ѝⲴ㺘䗮䟿᰾ᱮ儈Ҿ⿽ ᆀˈ䘉о CP4 EPSPS 㺘䗮䟿Ⲵ ELISA Ự⍻㔃᷌ᱟа㠤Ⲵ˄䱴㺘 6˅Ǆᡰᴹ䘉Ӌ ᶑᑖⲴ䗱〫⦷оབྷ㛐ᵶ㧼㺘䗮 CP4 EPSPS 㳻ⲭⲴ䗱〫⦷˄䱴മ 13ˈ⌣䚃 3˅ᱟ а㠤Ⲵˈ䇱ᇎҶ Western ঠ䘩ѝỰ⍻ࡠⲴ MON 87427 ਴цԓᶀᯉݽ⯛ᶑᑖ⺞ᇎ ᱟ CP4 EPSPS 㳻ⲭˈᒦф਴њцԓ⿽ᆀᶀᯉѝ㺘䗮䟿⴨լ˄䱴മ 13ˈ⌣䚃 5~8˅Ǆ ֌Ѫ䱤ᙗሩ➗ˈᑨ㿴ሩ➗ᶀᯉѝ⋑ᴹỰ⍻ࡠ CP4 EPSPS 㳻ⲭ˄䱴മ 13ˈ⌣䚃 10˅Ǆ Western ࠶᷀㔃᷌㺘᰾ˈMON 87427 Ⲵ CP4 EPSPS 㳻ⲭ൘н਼䚇Րцԓ䰤 ਟԕっᇊ㺘䗮Ǆ

169 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱴മ13. MON 87427ཊњцԓᶀᯉѝCP4 EPSPS㳻ⲭⲴWestern࠶᷀ MON 87427 Ⲵ 4 њцԓ⿽ᆀᶀᯉ઼ 1 њцԓਦ⡷ᶀᯉⲴㅹ䟿ᨀਆ⢙ԕ ৺࠶ᆀ䟿ḷ䇠䟷⭘ SDS-PAGE ࠶⿫ˈ❦ਾ⭥䖜〫㠣 PVDF 㟌Ǆሶ㟌оኡ㖺 ˖ᣇ CP4 EPSPS ᣇփᆥ㛢ˈ⭘ॆᆖਁݹ䈅ࡲᱮ㢢Ǆ⌣䚃䈤᰾ྲл

170 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 4. 䖢ะഖᙝ⣬਀ӝ⢟ⲺỶ⎁ૂ䢪ᇐᢶᵥ 4-1. MON 87427 䖜ॆփ⢩ᔲᙗࠍ㜦ᇊᙗ PCR Ự⍻ᯩ⌅˄CBI˅ 1˅ࠍ㜦ᇊᙗ PCR Ự⍻ᕅ⢙

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

2˅৽ᓄփ㌫઼৽ᓄ〻ᒿ MON 87427 ࠍ㜦ᇊᙗ PCR ᢙ໎৽ᓄփ㌫˖

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

hmgA ࠍ㜦ᇊᙗ PCR ᢙ໎৽ᓄփ㌫˖

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

2˅৽ᓄ〻ᒿ ᇊᙗ PCR ᢙ໎৽ᓄᗚ⧟ᶑԦ˄䘲⭘Ҿ MON 87427 ઼ hmgA PCR ৽ᓄ˅˖

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

3˅PCR 㔃᷌Ⲵਟ᧕ਇḷ߶ a. ᐢ⸕Ⲵ䱣ᙗṧ૱ᗵ享ӗ⭏亴ᵏⲴ PCR ӗ⢙˗ b. ᰐ⁑ᶯሩ➗઼ަᆳᐢ⸕Ⲵ䱤ᙗṧ૱ᗵ享Ự⍻нࡠ亴ᵏབྷሿⲴ PCR ӗ⢙Ǆ

171 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 4-2. MON 87427 䖜ॆփ⢩ᔲᙗᇎᰦᇊ䟿 TaqMan® PCR Ự⍻ᯩ⌅˄CBI˅ 1˅ᇊ䟿 PCR Ự⍻ᕅ⢙

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

2˅৽ᓄփ㌫઼৽ᓄ〻ᒿ MON 87427 Ⲵ PCR ৽ᓄ␧ਸ⏢˄অњ৽ᓄ˅

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

hmgA Ⲵ PCR ৽ᓄ␧ਸ⏢˄অњ৽ᓄ˅

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

ᇊ䟿 PCR ᢙ໎৽ᓄᗚ⧟ᶑԦ˄䘲⭘Ҿ MON 87427 ઼ hmgA PCR ৽ᓄ˅

ᔰ⡸ᵜѝᐢࡐ䲔ޜᇶ䍴ᯉˈ൘ᵜ؍ഐ⎹৺୶ъ

3˅ᇊ䟿ḷ߶ᴢ㓯Ⲵ㔈ࡦ઼䇑㇇ ሶ㓟ॆⲴᐢ⸕⎃ᓖ MON 87427 สഐ㓴 DNA ⓦ⏢оᐢ⸕⎃ᓖᑨ㿴⦹㊣สഐ 㓴 DNA ⓦ⏢␧ਸᶕࡦ༷ MON 87427 ḷ߶ DNA ⓦ⏢ǄMON 87427 Ⲵ DNA ḷ߶ ⓦ⏢⎃ᓖ࠶࡛Ѫ 50.0ǃ25.0ǃ5.0ǃ2.5ǃ0.5ǃ0.25ǃ0.05ǃ0.025 ઼ ˗⴨ᓄ Ⲵ MON 87427 สഐ㓴 DNA ൘ᙫ⦹㊣สഐ㓴 DNA ѝᡰঐ∄ֻ࠶࡛Ѫ 100%ǃ50%ǃ 10%ǃ5%ǃ1%ǃ0.5%ǃ0.1%ǃ0.05%઼ 0Ǆᡰᴹḷ߶ DNA ⓦ⏢ѝᙫⲴ⦹㊣ DNA ⎃ᓖᓄ䈕Ѫ Ǆ ᑨ㿴⦹㊣Ⲵḷ߶ DNA ⓦ⏢䟷⭘ሶ㓟ॆⲴᐢ⸕ᑨ㿴⦹㊣สഐ㓴 DNA оᐢ⸕ Ⲵᑨ㿴ަᆳ⢙⿽˄ྲỹ㣡ǃሿ哖ǃབྷ䉶ㅹ˅สഐ㓴 DNA ␧ਸᶕࡦ༷Ǆᑨ㿴⦹㊣ ḷ߶ DNA Ⲵ⎃ᓖ࠶࡛Ѫ 50.0ǃ25.0ǃ12.5ǃ5.0ǃ2.5 ઼ ˗⴨ᓄⲴ⦹㊣ส ഐ㓴 DNA ൘ᙫἽ⢙สഐ㓴 DNA ѝᡰঐ∄ֻ࠶࡛Ѫ 100%ǃ50%ǃ25%ǃ10%ǃ 5%ǃ1%઼ 0Ǆᡰᴹḷ߶ⓦ⏢ѝᙫⲴἽ⢙ DNA ⎃ᓖᓄ䈕Ѫ Ǆ ᐞˈᡰᴹḷ߶ⓦ⏢൷⭡਼аᢩ⅑㓿䗷ٿѪҶ᧗ࡦḷ߶ⓦ⏢䝽ࡦ䗷〻ѝⲴ䈅傼㦗ݹᇊ䟿Ⲵสഐ㓴 DNA ۘ⏢䝽ࡦӗ⭏Ǆ↔ཆ䙊䗷⭘਼аۘ⏢ởᓖ〰䟺ᗇࡠⲴн 䇱਴њ⎃ᓖḷ߶ⓦ⏢㓸փ〟䏣ཏབྷⲴ؍ᓖᐕ֌⏢䘋㹼ḷ߶ⓦ⏢䝽ࡦˈԕ৺⎃਼

172 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᯩ⌅ˈ䚯ݽሿփ〟ਆṧ䙐ᡀⲴਆṧ䈟ᐞǄ਼ᰦˈ䝽ࡦḷ߶ⓦ⏢ᡰ⭘Ⲵ࣐ṧᷚҏ 䇱ᡰᴹ DNA Ⲵа㠤ᙗˈк䘠ᡰᴹ DNA ḷ߶ⓦ⏢ࡦ؍㾱≲䙊䗷ѕṬṑ߶ǄѪҶ ⭘Ⲵ DNA ۘ⏢ᓄ䈕ᱟ䟷⭘⴨਼ᯩ⌅ᨀਆⲴǄ༷֯ ࡦ༷ PCR ৽ᓄփ㌫ᰦˈᔪ䇞൘ 37°C ≤⎤ѝ䀓߫䈅ࡲǄ䈅ࡲаᰖ䀓߫ˈᔪ䇞䇱৽ᓄ䰤Ⲵ਼аᙗ઼ሶӔ৹⊑ḃᴰሿ؍ᆈˈᒦ֯⭘ѻࡽᖫᓅ␧रǄѪҶ؍൘ߠк ॆˈ䲔Ҷ DNA ⁑ᶯঅ⤜࣐ޕࡠ⇿њ৽ᓄփ㌫ѝཆˈє⿽৽ᓄ␧ਸ⏢˄ањᱟѪ MON 87427 Ự⍻㘼ਖањᱟѪ hmgA Ự⍻˅ѝⲴަԆ䈅ࡲ㾱᤹➗䏣ཏޘ䜘ᢙ໎ ањ৽ᓄⲴ䟿˅а⅑ڊ৽ᓄⲴ䟿䇑㇇˄ྲ߶༷ањ৽ᓄ␧ਸ⏢ᰦ㾱㠣ቁཊ߶༷޽ ᙗޘ䜘࣐ޕǄMON 87427 Ⲵ PCR ઼ hmgA Ⲵ PCR ਟԕн൘਼аཙ䘋㹼Ǆ PCR ൘ ABI 7500 ᇎᰦᇊ䟿 PCR Ԛк䘋㹼ˈ᤹➗Ԛಘ⭏ӗল୶ᨀ׋Ⲵ䈤᰾Җ 䘋㹼᫽֌Ǆ96 ᆄ৽ᓄᶯ⇿ᆄѝ࣐ޕ৽ᓄ␧ਸ⢙ˈ❦ਾ⇿њ৽ᓄѝ࣐ޕ䘲䟿Ⲵ DNA ⁑ᶯˈⴆ㍗ⴆᆀǄѪҶᇊ䟿Ự⍻ṧ૱ѝ MON 87427 Ⲵ DNA ਜ਼䟿ˈ㾱ሩᡰᴹ MON 87427 ḷ߶ DNA ⓦ⏢䘋㹼 MON 87427 Ⲵ PCR ৽ᓄˈ਼ᰦ䘈㾱ሩᑨ㿴⦹㊣ ḷ߶ DNA ⓦ⏢䘋㹼 hmgA Ⲵ PCR ৽ᓄǄ⇿ԭṧ૱䜭ᓄ䈕䘋㹼к䘠єњ PCR ৽ ᓄǄ⇿њṧ૱䜭㾱ሩк䘠є⿽ PCR Ⲵ㔃᷌䘋㹼࠶᷀Ǆ⇿њ PCR 䜭ᓄवᤜ 1 њᐢ ⸕ਜ਼ᴹ 1% MON 87427 Ⲵሩ➗˄ਜ਼ 1% MON 87427 Ⲵ DNA ઼ 99%ᑨ㿴⦹㊣ DNA˅ǃ 1 њᐢ⸕Ⲵᑨ㿴⦹㊣ DNA ሩ➗઼ 1 њᰐ DNA ⁑ᶯⲴሩ➗Ǆḷ߶ DNA ⓦ⏢ǃỰ ⍻ṧ૱ԕ৺਴⿽ሩ➗Ⲵ PCR ৽ᓄ൷ᓄ䇮㖞єњԕкⲴ䟽༽Ǆ ࣐ޕᡰᴹ䈅ࡲࡠ৽ᓄᶯਾˈሩަ䘋㹼վ䙏⿫ᗳ˄ᔪ䇞 4°C 㠣ᇔ⑙઼ 250× g л⿫ᗳ 1 min˅ˈԕ֯ᡰᴹ⏢փ≷㚊൘৽ᓄᆄᓅ䜘Ǆ MON 87427 DNA Ⲵ⎃ᓖ઼ᙫ DNA Ⲵ⎃ᓖ˄˅ᱟ࡙⭘ MON 87427 PCR ઼ hmgA PCR ḷ߶ᴢ㓯ᶕ䇑㇇ⲴǄ ḷ߶ᴢ㓯䙊䗷ሩ⴨ᓄḷ߶ DNA ⓦ⏢⎃ᓖⲴ log 䖜ᦒ٬о⴨ᓄḷ߶ DNA ⓦ⏢ ٬Ⲵ൷٬䘋㹼㓯ᙗഎᖂ㔈ࡦǄഎᖂᴢ㓯Ⲵᯌ⦷㾱≲൘-3.6 ~ -3.1 ѻ䰤 ⅑䟽༽ Ct 3 ٬㾱˄㺘᰾൘䇮ᇊⲴ⎃ᓖ㤳ത 㺘᰾ PCR ᢙ໎᭸⦷൘ 90%~110%ѻ䰤˅ˈR2˄ ㌫˅Ǆޣ٬оᓅ⢙⎃ᓖሩᮠѻ䰤ާᴹᖸᕪⲴ㓯ᙗ ޵ˈPCR ৽ᓄᡰᗇ Ct ࡙⭘ྲлᯩ〻䇑㇇ MON 87427 Ⲵ DNA ⴨ሩҾᙫ DNA Ⲵ⴨ሩਜ਼䟿˖ MON 87427 DNA ঐᙫ DNA Ⲵ⴨ሩਜ਼䟿˄%˅= [MON 87427 DNA ⎃ᓖ/ᙫ DNA Ⲵ⎃ ᓖ] ×100 ࡔᯝањ PCR ᮠᦞᱟ੖ਟԕ᧕ਇˈᗵ享┑䏣лࡇḷ߶˖˄1˅ᐢ⸕Ⲵ 1% MON 87427 ሩ➗Ⲵ⴨ሩਜ਼䟿䇑㇇㔃᷌ᗵ享൘ 0.75%~1.25%ѻ䰤˗˄2˅ᐢ⸕ᑨ㿴 ˈ٬൘ MON 87427 PCR 亴䇮Ⲵ 40 њᗚ⧟޵ᰐਟỰ⍻ؑਧ ㊣ DNA ሩ➗Ⲵ Ct⦹ ٬൘ MON 87427 PCR ઼ hmg PCR ൘ hmg PCR ѝ<40˗˄3˅ᰐ⁑ᶯ DNA ሩ➗Ⲵ Ct 亴䇮Ⲵ 40 њᗚ⧟޵൷ᰐਟỰ⍻ؑਧǄ ⤜・ PCR ᇎ傼ѻ䰤Ⲵ 0ǄRSD Ѫ൘䟽༽ᶑԦлᗇࡠḷ߶䈟ᐞˈᱟ 䙊䗷ሶḷ߶䈟ᐞ˄SD˅䲔ԕ൷٬ᗇࡠⲴǄਈᔲ㌫ᮠ˄CV˅ҏᱟ䙊䗷ሶḷ߶䈟ᐞ 䲔ԕ൷٬ᗇࡠⲴˈо RSD ⴨਼ˈRSD о CV 䜭ԕⲮ࠶ᮠⲴᖒᔿ㺘⽪Ǆ

173 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 5. ਺䈋僂䱬⇫ᇗ᢯ҜⲺགྷদԬδ䘑ਙ䖢ะഖ⭕⢟⴪᧛⭩䈭ᆿޞ䇷Ҝ Ⲻθᵢ亯уມߏε ᵜ⭣䈧Ѫ䘋ਓ䖜สഐ⭏⢙ⴤ᧕⭣䈧ᆹޘ䇱Җˈᵜ亩н䘲⭘Ǆ

6. ਺䈋僂䱬⇫Ⲻᆿޞᙝ䇺ԭ䈋僂ᙱ㔉ᣛ઀δ䘑ਙ䖢ะഖ⭕⢟⴪᧛⭩ 䈭ᆿޞ䇷ҜⲺθᵢ亯уມߏε ᵜ⭣䈧Ѫ䘋ਓ䖜สഐ⭏⢙ⴤ᧕⭣䈧ᆹޘ䇱Җˈᵜ亩н䘲⭘Ǆ

7. 䖢ะഖὃ⢟ሯ⭕ᘷ⧥ູᆿޞᙝⲺ㔲ਾ䇺ԭᣛ઀ 䈧㿱л亥ǉ䖜 cp4 epsps สഐ㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣ MON 87427 Ⲵ⧟ຳ ઼伏⭘ᆹޘᙗ㔬ਸ䇴ԧᣕ੺ǊǄ

8. 伕૷ᆿޞᙝⲺ㔲ਾ䇺ԭᣛ઀θऻᤢ:Aεᗻ㾷Ⲻࣞ⢟∈⨼䈋僂ᣛ઀χ Bε伕૷䗽ᮅᙝ䇺ԭ䈋僂ᣛ઀χCεф䶔䖢ะഖὃ⢟∊䖹θެ㩛ޱ ᡆԳ਀ᣍ㩛ޱഖᆆ࠼᷆ᣛ઀ㅿ 䈧㿱л亥ǉ䖜 cp4 epsps สഐ㓴㓷⢩ᔲᙗᣇ㥹⭈㟖⦹㊣ MON 87427 Ⲵ⧟ຳ ઼伏⭘ᆹޘᙗ㔬ਸ䇴ԧᣕ੺ǊǄ

174 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䖢 cp4 epsps ะഖ㓺㓽⢯ᔸᙝᣍ㥿⭎㟜⦿㊩ MON 87427 Ⲻ⧥ູૂ伕⭞ᆿޞᙝ㔲ਾ䇺ԭᣛ઀ жȽ᪎㾷δ䖢䘦䖢ะഖ֒⢟Ⲻ䚍Ֆᙝ⣬Ƚ䈋僂ᒪ䲆Ƚ䇺ԭᡌỶ⎁᤽ḽ਀㔉䇰ε MON 87427 ࡟⭞Ҽ⢯ᇐⲺ੥ࣞᆆ-޻੡ᆆ㓺ਾδ e35S-hsp70εθ঩໔ᕰශ 35S ੥ࣞᆆૂ⦿㊩✣◶㴁ⲳ 70 ะഖⲺㅢжѠ޻੡ᆆȾ䈛੥ࣞᆆ -޻੡ᆆ㓺ਾਥԛ⢯ᔸ ᙝ൦൞㩛ޱ⭕䮵ಞᇎδਬ⡽Ƚ〮⿼ૂṯεૂ䴂ᙝ⭕⇌ಞᇎδਥԛ䘑ж↛ਇ㛨ᡆ〃 ᆆᡌ㊳㋈ૂ㣧ѓⲺ㓺㓽εѣ㺞䗴 CP4 EPSPS 㴁ⲳθֵ䘏ӑ㓺㓽ૂಞᇎਥԛᣍ㥿 ⭎㟜Ⱦռ൞њѠީ䭤Ⲻ䳺ᙝಞᇎδ㣧㊿ቅᆘᆆૂ∗㔈ቸ㓼㜔εѣ㺞䗴䠅ᶷքθࠖ ҄Ỷ⎁уࡦȾഖ↚θMON 87427 Ⲻ䳺ᙝಞᇎуᣍ㥿⭎㟜Ⱦ࡟⭞䈛⢯ᙝਥԛ൞⦿ ㊩〃ᆆ⭕ӝ䗽ぁѣθӰѰࡑ䙖䳺ᙝу㛨θ⭞ӄ⦿㊩ᵸӚ〃࡬〃Ⱦ MON 87427 ᱥ䙐䗽ߒᵼ㨂ԁሲ䖢ौθሼ PV-ZMAP1043 䍞㋈ѣⲺ T-DNA ሲ ޛࡦ LH198 × HiII ਍։૷㌱ѣ㧭ᗍⲺȾT-DNA ऻ੡ᴿжѠ cp4 epsps ะഖ㺞䗴ⴈθ 䈛ะഖ㺞䗴ⴈऻᤢ e35S ੥ࣞᆆȽhsp70 Ⲻ޻੡ᆆȽ CTP2 ਬ㔵։䖢〱㛳ᓅࡍȽ cp4 epsps 㕌⸷ᓅࡍૂ nos 㓾↘ᆆȾ Southern ᵸӚ࠼᷆㺞᱄θT-DNA ӻ൞⦿㊩ะഖ㓺жѠᓝփрᆎ൞ঋ᤭䍓ᨈ Ⲻ MON 87427ޛȾ䲚Ҽ T-DNA ཌθ䍞㋈Ⲻެᆹ䈹᧝ݹԬδऻᤢ僞ᷬᓅࡍε⋗ᴿᨈޛ Ⲻะഖ㓺ᓅࡍѣȾPCRૂDNA ᓅࡍ࠼᷆㔉᷒㺞᱄θ T-DNA ᨈޛⲺ㔉ᶺᱥᇂ᮪ⲺȽ 㘲ᓅࡍȾSouthernם㔉ᶺᱥ↙⺤Ⲻχᒬ࡟⭞ PCR ૂ DNA ᓅࡍ࠼᷆⺤ᇐҼะഖ㓺 ൞ 5 Ѡ㛨〃ьԙ䰪ؓᤷどᇐȾ↚ཌθሲޛᵸӚ࠼᷆䇷ᇔ MON 87427 ѣⲺཌⓆᨈ Ⲻ DNA ᓅࡍ൞ཐьԙ䰪ؓᤷどᇐȾޛᙝ⣬Ⲻ䚍Ֆ࠼⿱ᇔ僂㔉᷒ҕ䇷ᇔᨈޛ CP4 EPSPS 㴁ⲳ䍞⢯ᖷ⢯ᙝ䇺ԭ޻ᇯऻᤢφ 1ε⨼ौ⢯ᙝૂࣕ㜳࠼᷆χ2ε ൞уੂ㓺㓽ѣⲺ㺞䗴䠅χ3ε⭕⢟ؗᚥᆜ࠼᷆χ4 ൞⁗ᤕ㛹㛖⏨ᶗԬсⲺ⎾ौχ5ε ✣༺⨼どᇐᙝχ 6εᆿޞᓊ⭞়ਨχԛ਀ 7εሯରҩࣞ⢟▒൞ᙛᙝ∈ᙝᇔ僂Ⱦᡶ ᴿԛр䘏ӑ࠼᷆㔉᷒ൽ㺞᱄ MON 87427 㺞䗴Ⲻ CP4 EPSPS фཝ㛖ᵼ㨂㺞䗴Ⲻ ᓭ⋗ᴿ伄䲟Ⱦڛ⢟CP4 EPSPS 㴁ⲳㅿੂθሯӰૂࣞ ṯᦤ OECD ᤽঍θ࠼᷆Ҽ MON 87427 Ⲻ㊳㋈ૂ〮⿼Ⲻ㩛ޱᡆ࠼Ƚᣍ㩛ޱ ᡆ࠼ૂ↚⭕ԙ䉘ᡆ࠼Ⱦᡆ࠼࠼᷆㔉᷒㺞᱄θ MON 87427 Ⲻ਺〃ᡆ࠼ф䶔䖢ะഖ ሯ➝Ⲻᡆ࠼ᇔ䍞ㅿੂθᒬъൽ൞ᑮ㿺⦿㊩Ⲻ 99%ᇯ䇮॰䰪޻Ⱦ 〃ᆆՇⵖૂ㩂ਇ⢯ᙝૂ㣧㊿ᖘᘷ⢯ᖷⲺ䇺զ㔉᷒㺞᱄θMON 87427 уՐ໔ Ⱦ㺞ශᙝ⣬Ƚߒ㢰ᙝ⣬ૂф⧥ູӈ֒⹊ガⲺ⭦ੇٴࣖᵸ㥿ौᡌ䖢਎Ѱᴿᇩ⭕⢟Ⲻ 䰪䈋僂ӄ 2008 ᒪ൞㗄ള⦿㊩ᑜⲺ 5 Ѡ൦⛯䘑㺂ȾMON 87427 ૂ䶔䖢ะഖሯ➝൞ ࠖ҄ᡶᴿᙝ⣬Ⲻᡶᴿᮦᦤሯ∊рൽуᆎ൞㔕䇗ᆜᱴ㪍ᙝᐤᔸȾሯӄᶷቇᮦᆎ൞㔕䇗ᆜᱴ㪍ᙝᐤᔸⲺᙝ⣬θӂ㘻䰪Ⲻᐤᔸҕуޭᴿ⭕⢟ᆜᝅѿȾ ӂȽ㜂Ქԁ㔃 ᆕኧ䜳ޢਮᔶਇⲺ MON 87427 ࡟⭞Ҽ⢯ᇐⲺ੥ࣞᆆ - ޻੡ᆆ㓺ਾ δe35S-hsp70εθ঩໔ᕰශ 35S ੥ࣞᆆૂ⦿㊩✣◶㴁ⲳ 70 ะഖⲺㅢжѠ޻੡ᆆȾ 䈛੥ࣞᆆ-޻੡ᆆ㓺ਾਥԛ⢯ᔸᙝ൦൞㩛ޱ⭕䮵ಞᇎδਬ⡽Ƚ〮⿼ૂṯεૂ䴂ᙝ ⭕⇌ಞᇎδਥԛ䘑ж↛ਇ㛨ᡆ〃ᆆᡌ㊳㋈ૂ㣧ѓⲺ㓺㓽εѣ㺞䗴ߒᵼ㨂 CP4 㨂

175 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ṠⲺ 5-✥䞽я䞤䞦-3-⼭䞮䖢〱䞬δCP4 EPSPSεθֵ䘏ӑ㓺㓽ૂಞᇎਥԛᣍ㥿⭎ 㟜Ⱦռ䘏ж⢯ᇐⲺ੥ࣞᆆૂ޻੡ᆆ㓺ਾֵᗍ CP4 EPSPS 㴁ⲳ൞њѠީ䭤Ⲻ䳺ᙝ ಞᇎѣу㺞䗴ᡌ㺞䗴䠅ᴿ䲆φ㣧㊿ቅᆘᆆδਥԛ䘑ж↛ਇ㛨ᡆ㣧㊿㋈εૂ∗㔈ቸ εȾഖ↚θMON 87427 Ⲻ䳺ᙝಞᇎуᣍ㥿⭎㟜Ⱦޱ㩛ב㓼㜔δѰ㣧㊿㋈ਇ㛨ᨆ 䳺ろⲺ⭕䮵ਇ㛨ခӄ㩛ޱ⭕䮵Ⲻ V9 䱬⇫θഖ↚൞ MON 87427 ⭕䮵ਇ㛨Ⲻ V8-V13 䱬⇫њ⅗௭᯳㥿⭎㟜䲚㥿ࡸਥԛᵶ↱㣧㊿∃㓼㜔θӰᐛࡑ䙖䳺ᙝу㛨Ⱦ ࡟⭞੡ᴿ MON 87427 䖢ौ։Ⲻ㠠Ӛ㌱֒∃ᵢθ⭞੡ᴿެᆹᣍ㥿⭎㟜䖢ौ։Ⲻ㠠 ૂ〃Ӛ㌱֒⡬ᵢθਥԛᶺᡆӰᐛу㛨㌱࡬〃㌱㔕Ⱦ࡟⭞ MON 87427 䘑㺂⦿㊩࡬ 〃ᆆ⭕ӝⲺᝅѿ൞ӄφㅢжθ໔ࣖᵸӚ〃࡬〃Ⲻ⚫⍱ᙝȾ∃ᵢৱ䳺Ⲻީ䭤ᰬᵕᱥ Ԅ䳺ろᣳ࠰ࡦᮙ㊿䘏⇫ᰬᵕθᒩൽѰ 3-4 ཟȾᖉࢃӰᐛৱ䳺Ⲻᯯ⌋ऻᤢᵰỦᣳ䳺 δж㡢㾷 2 ⅗εૂᢁᐛᣳ䳺δж㡢 3 ⅗εȾу䇰ଠ〃ᯯ⌋θ䜳䴶㾷൞ 3-4 ཟⲺᰬ 䰪䠂䳼ѣ䏩ཕⲺӰ࣑ૂ䇴༽Ⱦ㙂䟽⭞ MON 87427 ࡬〃θਠ䴶㾷൞ V8-V13δཝ㓜 14 ཟεⲺᰬ䰪޻௭⍈њ⅗㥿⭎㟜䲚㥿ࡸȾഖ↚θ⦿㊩࡬〃Ⲻ⚫⍱ᙝᴪ儎Ⱦㅢӂθ ࠅቇᵸӚ〃࡬〃ⲺᡆᵢȾ䳅⵶㔅⎄ਇኋθ䏀ᶛ䏀ཐߒѐӰਙ䖢ੇคᐸθᒬъࣩࣞ ࣑ᡆᵢⵕ൞уᯣрॽθ䟽⭞Ӱᐛৱ䳺Ⲻᯯ⌋ሼՐ䶘Ѫ⭞ᐛ⸣㕰ૂᵸӚ〃〃ᆆᡆᵢ ⴮ሯ䖹儎Ⲻ᥇ᡎȾ㙂䟽⭞ MON 87427 ࡬〃㌱㔕Րᴿࣟӄ䀙ߩ䘏ӑ䰤从Ⱦ пȽ਍։⭕⢟ᆜ⢯ᙝ MON 87427 Ⲻ䖢ौ਍։ᱥѠᵸӚ〃θ㓺ਾѰ LH198 × HiIIȾLH198 ޭᴿ㢥 Ⲻߒ㢰ᙝ⣬χ㙂 HiII ሯߒᵼ㨂׫ḉ∊䖹ᮅ᝕θᒬъᇯ᱉߃⭕Ⱦ਍։ኔӄṳฯླ ⦿㊩θѰжᒪ⭕֒⢟Ⱦ൞࠼㊱ᆜрኔӄঋᆆਬὃ⢟㓨Ƚ⿴ᵢ〇δGramineaeεȽ⦿ 㴶唃ᰅδMaydeaeεȽ⦿㴶唃ኔδZea L.εȽ⦿㊩〃δZea mays LεȽṳฯ⦿㊩Ӑ〃 δmaysεȾ⦿㊩ᱥᴿᙝ㑷⇌֒⢟θᱥ㠠ӚȽᵸӚൽӨਾⲺ⢟〃Ⱦ㣧㊿ⲺՖ᫣ᯯᕅ ѱ㾷ᱥ伄჈Ֆ㊿θ㲡჈Ֆ㊿䖹ቇȾ⦿㊩ૂໞ㾵ଛ⦿㴶唃ޭᴿ䚍ՖਥӨਾᙝȾռໞ 㾵ଛ⦿㴶唃Ⲻ㠠❬࠼ᐹӻቶ䲆ӄ⋵⵶ໞ㾵ଛૂধ൦傢᣿㾵䜞Ⱦ⦿㊩ૂ᪟ᬜ⿴ኔਥ ᵸӚθռᶷѰу᱉ȾᒬъᵸӚ〃ޭᴿ儎ᓜⲺу㛨ᙝθ൞䚍ՖᆜрҕуどᇐȾ ࠖⲴᒪᶛθ⦿㊩ж⴪ᱥࣞ⢟侨ᯏૂӰ㊱Ⲻѱ伕Ⱦ⦿㊩㊳㋈ѣу੡∈㍖ૂ㠪 ᮅ㴁ⲳθ伕⭞⦿㊩ᰬਇ⭕Ⲻ䗽ᮅਃᓊᶷѰቇ㿷Ⱦռ⦿㊩੡ᴿࠖѠӰԢ⟕⸛Ⲻᣍ㩛 ޱഖᆆθऻᤢὃ䞮ȽзᐹȽỿᆆ㌌Ƚ㜦㴁ⲳ䞬ᣇ࡬ࡸૂ㜦ࠓҩ㴁ⲳ䞬ᣇ࡬ࡸȾ ታ㇗⦿㊩൞ޞь⮂ᒵ⌑〃ὃθռᱥӰԢᒬу䇚ѰᆹՐᡆѰ≮ѻᙝᵸ㥿ᡌ㘻䳴ӄ䱨⋱Ⲻᵸ㥿Ⱦ↙ྸᡇԢԀཟᡶ⸛θ⭧ӄ⦿㊩䴂ᙝ㣧ᓅδ᷒ろε㜳䲆࡬〃ᆆⲺ Ֆ᫣θഖ↚⦿㊩у㜳൞㠠❬⧥ູ䠂ᆎ⍱ ഑Ƚะഖᬃ֒ MON 87427 ᱥ䙐䗽ߒᵼ㨂ԁሲ䖢ौ⦿㊩ᒲ㜐θሼ PV-ZMAP1043 䍞㋈ѣⲺ T-DNA ሲޛࡦ LH198 × HiII ਍։૷㌱ѣ㧭ᗍⲺȾT-DNA ऻ੡жѠ⭧ e35S ੥ࣞᆆȽ hsp70 Ⲻ޻੡ᆆȽCTP2 ਬ㔵։䖢〱㛳ᓅࡍȽcp4 epsps ะഖ㕌⸷ᓅࡍૂ nos 㓾↘ ᆆᶺᡆⲺะഖ㺞䗴ⴈȾ Southern ᵸӚ㔉᷒㺞᱄θMON 87427 ਠᱥ൞ঋжփ⛯рᴿঋѠ᤭䍓Ⲻ T-DNA ᨈޛχᨈޛⲺ cp4 epsps ะഖ㺞䗴ⴈ൞㔉ᶺрᱥᇂ᮪ૂᴿᓅⲺχ䍞㋈Ⲻ僞 ᷬᓅࡍ⋗ᴿᨈޛࡦ⦿㊩ะഖ㓺ѣȾ

176 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ࡟⭞ PCR ᯯ⌋ᢟ໔Ҽ 3 Ѡ⴮ӈ䠃ਖⲺ DNA ⡽⇫θ䘏 3 Ѡ⡽⇫⏫ⴌҼޞ䮵㘲ะഖ㓺ᓅࡍȾሼ䘏 3 Ѡᢟ໔ӝ⢟䘑㺂ᓅࡍ࠼᷆ᒬᤲ᧛θ঩ਥ⺤ᇐםᓅࡍૂޛᨈ ᓅࡍཝቅѰ 3746 bpθެѣޛ㘲ᓅࡍȾMON 87427 Ⲻᨈםᓅࡍ਀ะഖ㓺ޛ䮵ᨈޞ T-DNA ᨈޛѰ 3681 bpȾਜཌ൞ T-DNA Ⲻ 5'ㄥૂ 3'ㄥ࠼ࡡᴿ 41 bp ૂ 24 bp Ⲻᨈ փ⛯䘑ޛགྷᵰ࡬ᕋ䎭Ⱦ䪾ሯᨈؤ䗽ぁѣਂ䬴ᯣ㻸ޛθ䘏ਥ㜳ᱥ⭧ӄ T-DNA ᨈޛ 㺂Ⲻ PCR ࠼᷆㔉᷒㺞᱄θ൞ T-DNA ᨈޛᰬ਍։ะഖ㓺 DNA ᴿ 140 Ѡ⻧ะ㻡ࡖ 䲚Ⱦռ䘏⇫ࡖ䲚ᓅࡍуޭᴿ⢯ᇐⲺ⭕⢟ᆜࣕ㜳Ⱦ ELISA ࠼᷆㔉᷒㺞᱄θMON 87427 Ⲻуੂ㓺㓽ૂಞᇎѣ CP4 EPSPS 㴁ⲳⲺ μg/g dwtȾެ 940 ٲքӄỶ࠰䲆δLODεࡦᴶཝٲ㺞䗴䠅਎ौ㤹പ䖹ཝθԄᴶቅ ѣਬ⡽ѣⲺᒩൽ㺞䗴䠅ᴶ儎δ㤹പԄ OSL3 Ⲻ 290 μg/g dwt ࡦ OSL1 Ⲻ δ㤹പԄ OSWP4 Ⲻ 240 μg/g dwt ࡦ OSWP1ٲμg/g dwtεχެ⅗ᱥ᮪Ṡᒩൽ 680 Ѱṯ㌱δԄ OSR3 Ⲻ 73 μg/g dwt ࡦ OSR1 Ⲻ⅗דⲺ 500 μg/g dwtεȾԛс 140 μg/g dwtεȽ൦р䜞᮪Ṡδ120 μg/g dwtεȽ㣧ѓδ100 μg/g dwtεȽ≊⭕ṯ δ72 μg/g dwtεȽ㺦㘷ṯ㌱δ72 μg/g dwtεȽ㥄ਬδ43 μg/g dwtεૂ㊳㋈δ4.2 μg/g dwtεȾ ᴿ 6 㣧㊿ṭ૷δᶛ㠠 3 Ѡ䈋僂⛯εⲺ CP4 EPSPS 㴁ⲳ㺞䗴䠅քӄỶ࠰䲆δ

178 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 Ⓠф਍։Ⲻ䐓⿱䎻䗽 200 mθਇ⭕ะഖ┸〱Ⲻਥ㜳ᙝቧՐ䲃ࡦ 1%ԛсȾ⦿㊩ૂ жᒪ⭕ໞ㾵ଛ㊱㴶唃δZea mays subsp. mexicanaεໞ㾵ଛૂধ൦傢᣿Ⲻжӑ൦॰ ྸ᷒〃ὃ䐓⿱ᖾ䘇θਥਇ⭕㠠❬⴮ӈᦾ㊿ᒬ㺞⧦ᵸӚӨૂᙝȾռᴿ⹊ガ㺞᱄θ঩ ሼ⦿㊩ૂໞ㾵ଛ⦿㴶唃〃൞ж䎭θӂ㘻ҁ䰪ਇ⭕ᔸӚⲺ仇⦽䘎ᱥᖾքⲺȾྸֵ᷒ 㘹㲇ᔶ㣧ᰬᵕȽ൦⨼䐓⿱䳊⿱ૂਇ㛨ぁᓜㅿഖ㍖θӂ㘻уའਥ㜳ਇ⭕㠠❬ᔸӚȾ ㊿㊩ф᪟ᬜ⿴ኔδTripsacumεⲺཐᒪ⭕⢟〃ⲺᵸӚ䶔ᑮദ䳴Ⱦ↚ཌθ঩ֵᦾ⦿ ᵸӚᡆࣕθެ੄ԙҕՐу㛨ᒬъ൞䚍ՖрҕᱥуどᇐⲺȾ㠩ӄ MON 87427 ੇެ ᆹ⦿㊩ਇ⭕ะഖ┸〱уՐᕋ䎭ᆿޞᯯ䶘Ⲻ亴㲇θഖѰཝ䠅⹊ガᐨ㔅䇷᱄ ᑜᶛধᇩȾޞᓭૂ⧥ູᆿڛ㊱MON 87427 ऻ੡Ⲻ cp4 epsps ะഖуՐሯӰ йȽ伕⭞ᆿޞ䇺ԭ 㩛ޱᆜ䇺ԭφԄ 2008 ᒪ㗄ള 3 Ѡ൦⛯Ⲻ⭦䰪䈋僂ѣ䟽䳼ṭ૷䘑㺂㩛ޱᡆ࠼ ࠼᷆Ⱦ࠼᷆Ⲻᡆ࠼ऻᤢ㩛ޱᡆ࠼Ƚᣍ㩛ޱᡆ࠼ૂ⅗⭕ԙ䉘⢟䍞Ⱦ㩛ޱᡆ࠼ऻᤢφ ㊳㋈ѣⲺᑮ㿺ᡆ࠼δ⚦࠼Ƚᣎ㇍Ⲻ⻩≪ौਾ⢟Ƚ≪࠼Ƚ㴁ⲳ䍞ૂ㜸㛠εȽ䞮ᙝ⍍ ⏚㓚㔪δADFεȽѣᙝ⍍⏚㓚㔪δNDFεȽᙱ㟩伕㓚㔪δTDFεȽ≞ะ䞮Ƚ㜸㛠䞮 δC8~C22εȽ⸵⢟䍞δ䫏Ƚ䬒Ƚ䫷Ƚ䭷Ƚ䭦Ƚ⼭Ƚ䫴Ƚ䫖ૂ䭂εૂ㔪⭕㍖δਬ䞮Ƚ ✕䞮Ƚ㔪⭕㍖ Aδ!㜗㩓঒㍖εȽ㔪⭕㍖ B1Ƚ㔪⭕㍖ B2Ƚ㔪⭕㍖ B6 ૂ㔪⭕㍖ Eε ԛ਀൦р䜞ὃṠⲺᑮ㿺ᡆ࠼ȽADFȽNDFȽ䫏ૂ⼭Ⱦᣍ㩛ޱᡆ࠼ऻᤢὃ䞮ૂỿ㊳ ٲ㌌Ⱦ⅗⭕ԙ䉘ӝ⢟ऻᤢ㌖䟑Ƚ䱵内䞮ૂሯ-俏䉼䞮Ⱦ4 Ѡ୼ѐौ৸➝૷〃Ⲻᒩൽ ⭞ᶛ⺤ᇐḆ〃ᡆ࠼Ⲻ 99%ᇯ䇮॰䰪Ⱦ൞࠼᷆Ⲻ 78 〃ᡆ࠼ѣθᴿ 16 〃㊳㋈ᡆ࠼δ15 〃㩛ޱᡆ࠼ૂ 1 〃ᣍ㩛ޱᡆ࠼ε䎻䗽 50%Ⲻ࠼᷆⋗ᴿ䗴ࡦᇐ䠅с䲆δLOQεθᰖ ⌋䘑㺂㔕䇗࠼᷆Ⱦഖ↚ਠሯެᆹᡆ࠼䘑㺂Ҽ㔕䇗࠼᷆Ⱦ൞䘑㺂㔕䇗࠼Ⲻᡆ࠼ѣθ MON 87427 ૂᑮ㿺ሯ➝൞ཝཐᮦᡆ࠼руᆎ൞㔕䇗ᆜᱴ㪍ᐤᔸȾቇ䠅ᆎ൞ᱴ㪍㩳൞୼ѐौٲᐤᔸⲺᡆ࠼θ㾷Ѿᐤᔸ㺞⧦уж㠪θ㾷Ѿᐤᔸᖾቅθ㾷Ѿ࠼᷆ᒩൽ ૷〃Ⲻ 99%ᇯ䇮॰䰪޻ᡌ㘻㩳൞ ILSI ᮦᦤᓉᡌਇ㺞ᮽ⥤Ⲻ䈛ᡆ࠼ᮦᦤ॰䰪޻θ ᙱҁൽуޭᴿ⭕⢟ᆜᝅѿȾഖ↚θਥԛᗍ࠰㔉䇰䇚Ѱ MON 87427 ૂᑮ㿺ሯ➝൞ 㩛ޱᡆ࠼рᇔ䍞ㅿੂȾཟ⍛⯴⯻人䱨᧝࡬ѣᗹሯ MON 87427 ㊳㋈䘑㺂Ⲻᣍ㩛 ޱഖᆆᡆ࠼Ỷ⎁㔉᷒ҕ㺞᱄θ䖢 cp4 epsps ะഖᣍ䲚㥿ࡸ⦿㊩ MON 87427 ѣᣍ㩛 ޱഖᆆὃ䞮੡䠅Ѱ 20.3 mg/gθ䶔䖢ะഖᑮ㿺⦿㊩ EXP262 ѣὃ䞮੡䠅Ѱ 20.2 mg/gθ њ㘻⋗ᴿᱴ㪍ᐤᔸȾ ∈⨼ᆜ䇺ԭφCP4 EPSPS 㴁ⲳᶛⓆӄൕ༚ߒᵼ㨂θᱥ㠠❬⮂ѣὃ⢟ૂᗤ⭕ ⢟Ფ䚃ޭᴿⲺ EPSPS 㴁ⲳⲺж〃θޭᴿ䮵ᵕᆿޞᓊ⭞়ਨȾ㴁ⲳ⨼ौ⢯ᖷ⢯ᙝ ⹊ガ㔉᷒㺞᱄θMON 87427 ᶛⓆⲺ CP4 EPSPS 㴁ⲳфཝ㛖ᵼ㨂ᶛⓆⲺㅿੂȾ⭕ ૂ ؗᚥᆜ࠼᷆㔉᷒㺞᱄θMON 87427 㺞䗴Ⲻ CP4 EPSPS 㴁ⲳф TOX_2016⢟ PRT_2016 ᮦᦤᓉѣᐨ⸛Ⲻ∈㍖㴁ⲳૂᣍ㩛ޱഖᆆ⋗ᴿᓅࡍૂ㔉ᶺрⲺ⴮ղᙝȾ ሯ 10 ਠ䳺ᙝૂ 10 ਠ䴂ᙝ CD-1 ቅ啖䘑㺂 CP4 EPSPS 㴁ⲳ⚂㛹θ䟽⭞ 3 Ѡࡸ䠅δ࠼ ࡡѰ 49Ƚ154 ૂ 572 mg/kg bwtε༺⨼Ⱦグⲳሯ➝㓺⭞ 50 mM ⻩䞮≘䫖㕉ߨ⏨༺ ⨼䳺ᙝૂ䴂ᙝቅ啖਺ 10 ਠθࡸ䠅Ѱ 33.33 ml/kg bwtȾ䱪ᙝሯ➝㓺⭞⢑㹶␻ⲳ㴁 ⲳδBSAε༺⨼ 10 ਠ䳺ᙝૂ 10 ਠ䴂ᙝ CD-1 ቅ啖θ⚂㛹ࡸ䠅Ѱ 363 mg/kg bwtȾ ⎁ᓭ⣬߫Ⱦ∅ઞ䘑㺂ж⅗䈜㓼ⲺѪᓀ㿸ሕᒬڛૂ⦽ᇔ僂ᵕ䰪θ∅ཟњ⅗䈹ḛ↱ӗ ᇐ։䠃ૂ䘑伕䠅Ⱦ⭧ӄ⋗ᴿ㿸ሕࡦу㢥ਃᓊθㅢ 8 ཟ㔉ᶕ䈋僂θሼᡶᴿቅ啖䀙ࢌ 䘑㺂⯻⨼ᆜ㿸ሕȾᇔ僂ѣ⋗ᴿਇ⧦ CP4 EPSPS 㴁ⲳ༺⨼ሯӄቅ啖Ⲻᆎ⍱⦽ȽѪ

179 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᓀ㺞⧦Ƚ໔䠃Ƚ侨ᯏ੮᭬࡟⭞ᡌᙱⲺ⯻⨼ᆜ㿸ሕᴿӶѾу࡟ᖧଃθཝ։䀙ࢌҕᵠ ਇ⧦㝅ಞᔸᑮȾCP4 EPSPS 㴁ⲳԛᇔ僂ѣⲺᴶ儎ࡸ䠅 572 mg/kg bwt ⚂㛹ᰬθ⋗ ᴿሯቅ啖ӝ⭕Ա֋у࡟ᖧଃȾഖ↚θCP4 EPSPS Ⲻᰖ᱄ᱴᦕᇩ֒⭞ࡸ䠅δNOAELε 㠩ቇѰ 572 mg/kg bwtȾṯᦤ↚ NOAEL ԛ਀ѣളӰ㗚㩛ޱ᩺ޛ≪ᒩ䈹ḛᮦᦤθ ਥԛ䇗㇍࠰䙐䗽㟩伕᩺ޛ MON 87427 ѣ CP4 EPSPS 㴁ⲳⲺᳪ䵨䲆δMOEε儎䗴 1.4 × 104~7.9 × 105Ⱦഖ↚θMON 87427 ѣⲺ CP4 EPSPS 㴁ⲳуՐሯӰ㊱ૂࣞ ⢟ӝ⭕∈ᙝȾ 2016 ᒪθ⭧ӄߒѐ䜞䇺ᇗщᇬཐ⅗ሯ CP4 EPSPS 㴁ⲳቅ啖ᙛᙝ∈ᙝ⹊ガᵠ ћṲ䚫ᗠߒѐ䜞ީӄᙛᙝ∈ᙝ䇺ԭᯯ⌋㺞⽰亴㲇θᆕኧ䜳ޢਮ߃⅗ሯ CP4 EPSPS 㴁ⲳ䘑㺂Ҽቅ啖㔅ਙ⚂㛹ᙛᙝ∈ᙝ䈋僂Ⱦ࡟⭞ཝ㛖ᵼ㨂㺞䗴㓥ौⲺ CP4 EPSPS 㴁ⲳԛ㔅ਙᯯᕅԛ 5000 mg/kg ։䠃ࡸ䠅ঋᰛњ⅗⚂㛹䴂䳺਺ 10 ਠ CD-1 ቅ啖Ⱦ ੂᰬ䘑㺂䈋僂Ⲻ䘎ᴿ⓬ࡸሯ➝㓺ԛ਀ԛੂㅿࡸ䠅ቅ⢑㹶␻㴁ⲳδBSAε⚂㛹Ⲻ㴁 ⲳሯ➝㓺Ⱦᡶᴿࣞ⢟൞⚂㛹ࢃൽ⾷伕༺⨼θ⚂㛹੄∅ཟњ⅗㿸ሕቅ啖ᱥੜ↱ӗᡌ ᱥ㺞⧦࠰ѣ∈⯽⣬θ㿸ሕᵕѰ 14 ཟȾㅢ 14 ཟᰬሼᡶᴿࣞ⢟༺↱θ䘑㺂ཝ։䀙ࢌȾ ⹊ガ㔉᷒㺞᱄θᰖ䇰Ԅቅ啖ᆎ⍱⦽ȽѪᓀ㺞⧦Ƚ։䠃Ƚ։䠃໔ࣖȽ伕⢟⎾㙍ᡌཝ ։⯻⨼ᆜᯯ䶘ⵁθൽᵠਇ⧦ф༺⨼⴮ީⲺу㢥ਃᓊȾഖ↚θ䙐䗽䈛ቅ啖ᙛᙝ∈ᙝ ⹊ガᗍࡦⲺ CP4 EPSPS 㴁ⲳⲺᰖ᱄ᱴᦕᇩ֒⭞ࡸ䠅δNOAELε㠩ቇѰ⹊ガᴶ儎 ࡸ䠅 5000 mg/kg bwθ⭧↚զ㇍ᗍࡦ䙐䗽㟩伕᩺ޛ MON 87427 ѣ CP4 EPSPS 㴁 ⲳⲺᳪ䵨䲆δMOEε儎䗴 6.9 × 106Ⱦ ਍ߒѐ䜞ညᢎθཟ⍛⯴⯻人䱨᧝࡬ѣᗹሯ MON 87427 䘑㺂Ҽ伕⭞ᆿޞỶ⎁Ⱦ ሼ䖢 cp4 epsps ะഖᣍ䲚㥿ࡸ⦿㊩ MON 87427 ૂ䶔䖢ะഖӨᵢ⦿㊩ EXP262 ࠼ࡡ 侨ᯏδ∊ׁѰ 50% Ƚ25% Ƚ12.5%εஸ侨ཝ啖 90 ཟθஸ侨ᵕ䰪ࣞ⢟⍱ࣞ㠠ޛ᧰ θ㻡∑ᴿݿ⌳θ啱Ƚ⵲Ƚਙ㞊ᰖᔸᑮ࠼⌂⢟Ⱦ㔕䇗㔉᷒㺞᱄䜞࠼ᑮ㿺ሯ➝਀䖢ྸ ะഖ਍䈋⢟㓺ࣞ⢟䘮伕䠅фᑮ㿺ะ⹶侨ᯏሯ➝㓺∊䖹θᴿ㔕䇗ᆜᝅѿδP<0.05εθ ռൽᰖࡸ䠅ਃᓊީ㌱θᵠ㿷ެ⭕⢟ᆜᝅѿȾެ֏਺㓺ࣞ⢟։䠃Ƚ䘑伕䠅Ƚᙱ໔䠃Ƚ ᙱ䘑伕䠅ૂᙱ伕⢟࡟⭞⦽фᑮ㿺ะ⹶侨ᯏሯ➝㓺∊䖹θᐤᔸൽᰖ㔕䇗ᆜᝅѿ δP>0.05εȾ ᇔ僂ѣᵕૂᇔ僂ᵡᵕ਺亯㹶⏨ᆜ᤽ḽδ㓘㓼㜔䇗ᮦȽ㹶㓘㴁ⲳȽ㹶ቅᶵȽ ⲳ㓼㜔䇗ᮦ਀࠼㊱εȽ㹶⭕ौ᤽ḽδ㹶␻я≞䞮≞ะ䖢〱䞬Ƚཟ䰞ߢ≞䞮≞ะ䖢〱䞬Ƚት㍖≤Ƚ㛼ര䞽Ƚ⭎⋯п䞥Ƚ㹶㌌Ƚҩ䞮㝧≘䞬Ƚᙱ㴁ⲳȽⲳ㴁ⲳȽ㛂䞆Ƚ 㤹പ޻Ⱦࣞ⢟䀙ࢌᰬθཝ։㿸ሕᵠਇ⧦ᔸᑮȾٲᙝ⼭䞮䞬εൽ൞ᵢỶ⎁ঋփ↙ᑮ⻧䳺ᙝࣞ⢟Өѣ㓺㛴/։∊քӄሯ➝㓺θӨ儎㓺㛓/։∊儎ӄሯ➝㓺θ䖢ք㓺㛓/։∊ 儎ӄሯ➝㓺θ䖢ք㓺ᗹ/։∊儎ӄӨք㓺θ䖢ѣ㓺㛴р㞰/։∊儎ӄӨѣ㓺θᐤᔸ ᴿ㔕䇗ᆜᝅѿδP<0.05εȾ䴂ᙝࣞ⢟Ө儎㓺㜮㞰/։∊Ƚ㛴/։∊儎ӄሯ➝㓺θ䖢 ѣ㓺㛓/։儎ӄሯ➝㓺θ䖢儎㓺㜮㞰/։∊Ƚ㛴/։∊儎ӄሯ➝㓺θ䖢ѣ㓺㛓/։儎ӄ Өѣ㓺θᐤᔸᴿ㔕䇗ᆜᝅѿδP<0.05εȾռр䘦ᐤᔸᰖࡸ䠅ਃᓊީ㌱θᵠ㿷ެ⭕ фሯ➝㓺∊䖹θᐤᔸൽᰖ㔕䇗ᆜٲ∍ᆜᝅѿȾެ֏਺㓺ࣞ⢟㝅ಞ䠃䠅ૂ㝅/։⢟ ᝅѿδP<0.05εȾ⯻⨼㓺㓽ᆜỶḛᵠਇ⧦䈛䖢ะഖṭ૷ᕋ䎭ᇔ僂ࣞ⢟ޮශ⯻⨼㓺㓽ᆜ᭯਎ૂ⢯ᔸᙝᦕ՚Ⱦ 㠪ᮅᙝ䇺ԭφMON 87427 ㊳㋈ѣ㺞䗴Ⲻ CP4 EPSPS 㴁ⲳᒩൽ≪ᒩᱥ θ㙂㊳㋈ѣᒩൽᙱ㴁ⲳ੡䠅Ⲵ࠼ᮦѰ㊳㋈ᒨ䠃Ⲻ 10.05αδᡌ㘻䈪

180 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 100500 μg/g dwtεȾ䇗㇍ᗍࡦ CP4 EPSPS 㴁ⲳখ㊳㋈ᙱ㴁ⲳⲺ੡䠅Ѱ 0.004αθ ҕቧᱥখ㊳㋈ᙱ㴁ⲳⲺ∊ׁᖾቅȾ⭕⢟ؗᚥᆜ࠼᷆㔉᷒㺞᱄θMON 87427 㺞䗴 Ⲻ CP4 EPSPS 㴁ⲳф AD_2016 ᮦᦤᓉѣᐨ⸛Ⲻ㠪ᮅ㴁ⲳԛ਀ެᆹ⍱ᙝ㴁ⲳ⋗ᴿ ᓅࡍૂ㔉ᶺрⲺ⴮ղᙝȾ⁗ᤕ⎾ौᇔ僂㔉᷒㺞᱄θཝ㛖ᵼ㨂ӝ⭕Ⲻ CP4 EPSPS 㴁ⲳ SGF 䠂䗻䙕㻡⎾ौȾṯᦤ SDS-PAGE ࠓ㜬ḉ㢨ૂ Western ࠼᷆㔉᷒θ࠼ࡡ ᴿ 98αૂ 95%ԛрⲺ CP4 EPSPS 㴁ⲳ൞ 15 s ޻㻡⎾ौȾੂṭθCP4 EPSPS 㴁ⲳ ൞ SGF 䠂ฯޱуࡦ 15 sθެ⍱ᙝ䲃քࡦ 10αԛсȾCP4 EPSPS 㴁ⲳⲺ✣どᇐᙝ ᇔ僂㔉᷒㺞᱄θᖉԛ 25ºCȽ37ºC ૂ 55ºC ࣖ✣ 15 min ੄㿸ሕࡦ CP4 EPSPS ⍱ᙝ ぃᴿ䲃քθ࠼ࡡ䲃㠩ሯ➝Ⲻ 81%Ƚ84%ૂ 70%χռࣖ✣ 30 min ੄θ25ºC ༺⨼Ⲻ CP4 EPSPS ⍱ᙝ⋗ᴿ਎ौθ37ºC ༺⨼㓺Ⲻ䞬⍱䲃㠩ሯ➝ⲺⲺ 88%θ㙂 55ºC ༺⨼ 㓺Ⲻ CP4 EPSPS 㺞⧦Ѱཝᑻс䲃θӻѰሯ➝Ⲻ 25%Ⱦу䇰ᱥ༺⨼ 15 min 䘎ᱥ༺ ⨼ 30 minθ75°C ૂ 95°C ⑟ᓜᶗԬсⲺ CP4 EPSPS 䞬⍱ൽքӄỶ⎁䲆ȾSDS-PAGE ࠼᷆㔉᷒㺞᱄θ൞ᡶᴿ༺⨼ᰬ䰪ૂ༺⨼⑟ᓜᶗԬсθṭ૷Ⲻ 43.8 kDa ᶗᑜؗਭ ᕰᓜ⋗ᴿ᱄ᱴⲺ䲃քθ㺞᱄ࣖ✣༺⨼ӻՐֵ CP4 EPSPS 㴁ⲳⲺ䞬⍱ᙝ䲃քθռ уՐֵ CP4 EPSPS 㴁ⲳ䲃䀙Ⱦ㔲рᡶ䘦θCP4 EPSPS 㴁ⲳуՐᕋ䎭ᴪཐⲺ㠪ᮅ ᯯ䶘Ⲻ亴㲇Ⱦ ᣍ⭕㍖ᣍᙝφMON 87427 у੡Ա֋ᣍ⭕㍖ᣍᙝḽ䇦ะഖȾ ޡȽ䶔人ᵕ᭾ᓊ MON 87427 ѣሲޛⲺะഖᱥᶛⓆӄൕ༚ߒᵼ㨂 CP4 㨂ṠⲺ cp4 epsps ะഖθ ਥԛ㺞䗴 CP4 EPSPS 㴁ⲳȾEPSPS ൞ὃ⢟ૂᗤ⭕⢟ѣᲤ䚃ᆎ൞θᱥ㣩俏ᰅ≞ะ 䞮ਾᡆ䙊ᖺрⲺީ䭤䞬θެ֒⭞ᵰ࡬ᖾ␻ᾐȾCP4 EPSPS ᱥ EPSPS 㴁ⲳᇬᰅѣ Ⲻж઎θфὃ⢟޻Ⓠ EPSPS ᴿᖾ儎ੂⓆᙝθ֒⭞ᵰ࡬⴮ੂθռޭᴿф㥿⭎㟜у ӨૂⲺ⢯ᙝȾഖ↚θԄሲޛཌⓆะഖ䀈ᓜᶛ䈪θਥԛ人ᵕ MON 87427 ᕋޛⲺ᯦ ᙝ⣬уՐᑜᶛ䶔人ᵕ᭾ᓊȾ ࠼ᆆ⢯ᖷ⢯ᙝ࠼᷆Ƚ㺞ශᙝ⣬ૂߒ㢰ᙝ⣬࠼᷆ԛ਀㓺ᡆᡆ࠼࠼᷆Ⲻ㔉᷒ҕ 㺞᱄θMON 87427 ᒬ⋗ᴿഖѰሲޛ CP4 EPSPS 㴁ⲳ㙂ᑜᶛ䶔人ᵕ᭾ᓊȾ ғȽ⭕ӝᓊ⭞ࢃᲥ਀ᤕ䟽਌Ⲻᆿޞⴇ᧝᧠᯳ MON 87427 ሼ⭞ӄᶺᔰ᯦Ⲻ⦿㊩ᵸӚ〃࡬〃㌱㔕ȾᴶᰟՐ൞㗄ളૂࣖᤵཝ ᔶခ୼ѐौᓊ⭞θਥԛ人ᵕሼᶛՐᲤ䚃ᆎ൞ӄ਺⦿㊩ᵸӚ〃ѣȾռᵢ⭩䈭ӻ䲆ӄ 䇷ҜȾ֒Ѱ MON 87427 Ⲻᢶᵥ⹊ਇ୼θᆕޞ൞ѣള㧭ᗍ䘑ਙ⭞֒ࣖᐛ৕ᯏⲺᆿ ኧ䜳ޢਮՐ㾷≸䍮᱉୼ћṲ䟽਌᧠᯳θ䚫ᆾ⴮ީ⌋ᗁ⌋㿺θऻ㻻䘆䗉㾷ਥ䶖θḽ ᢶᵥ᤽ሲૂ᭥ᤷθԛؓ䇷ב䇼㾷␻ᾐθ䘆䗉਺⧥㢸ћṲᬃ֒θᗻ㾷ᰬੇ䍮᱉୼ᨆ MON 87427 Ⲻ䍮᱉ㅜਾߒѐ䜞亷ਇⲺᆿޞ䇷ҜⲺ㾷≸Ⱦ ॷȽ㔉䇰 ᴿ䍕䶘ᖧଃθ⋖ޞᓭૂ⧥ູᆿڛ㊱ཝ䠅Ⲻ⹊ガ㔉᷒㺞᱄θMON 87427 ሯӰ ф਍։ૂᑮ㿺⦿㊩ੂṭᆿޞȾ

181 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 9. 䈛㊱䖢ะഖὃ⢟ള޻ཌ⭕ӝᓊ⭞Ᾰ߫ 2016 ᒤᱟ䖜สഐ֌⢙୶ъॆㅜ 21 ᒤǄ൘ 2014 ᒤˈ࡙⭘⭏⢙ᢰᵟᔰਁⲴ䖜 สഐ֌⢙൘ޘ⨳Ⲵ⿽Ἵ䶒〟䗮ࡠ 1.81 ӯޜ产ˈ∄ 2013 ᒤⲴ 1.752 ӯޜ产໎䮯Ҷ 3.6ˁ˗Ӿ 1996 ᒤⲴ 170 зޜ产ࡠ 2014 ᒤⲴ 1.815 ӯޜ产ˈޘ⨳䖜สഐ֌⢙Ⲵ⿽ Ἵ䶒〟໎䮯Ҷ 100 ཊؽˈ䘉а໎䮯֯ᗇ䖜สഐᢰᵟᡀѪ⧠ԓߌъਢкᓄ⭘ᴰѪ䗵䙏Ⲵ֌⢙ᢰᵟǄ 2013 ᒤ 10 ᴸ 30 ᰕˈ䲿⵰ 4 њ䖜สഐᣇ㲛㤴ᆀ૱⿽㧧߶⭘Ҿ⿽ᆀ⭏ӗ઼୶ ъॆ⿽Ἵˈᆏ࣐᣹ഭᡀѪޘ⨳ㅜ 28 њ⿽Ἵ䖜สഐ֌⢙ⲴഭᇦǄ㖾ഭǃᐤ㾯ǃ䱯 ṩᔧǃঠᓖǃ࣐᤯བྷ઼ѝഭӽ❦ᱟᴰѫ㾱䖜สഐ֌⢙⿽ἽഭᇦǄޡᴹ 19 њ䖜ส ഐ֌⢙⿽ἽབྷഭⲴ⿽Ἵ䶒〟൘ 5 зޜ产ԕкǄᒦфˈ䲿⵰䎺ই઼ঠᓖቬ㾯ӊҾ 2014 ᒤ࠶࡛ᢩ߶Ҷ䖜สഐ⦹㊣઼⭈㭇Ⲵ୶ъॆ⿽Ἵˈᴹᵋ 2015 ᒤޘ⨳䖜สഐ֌ ⢙⿽Ἵഭᇦ䗮ࡠ 30 њǄ ቡ䖜สഐᙗ⣦㘼䀰ˈӾ 1996 ᒤ䖜สഐ֌⢙俆⅑୶ъॆ㠣Ӻˈᣇ䲔㥹ࡲᙗ⣦ ࿻㓸ᱟ䖜สഐ֌⢙Ⲵѫ㾱ᙗ⣦Ǆ2014 ᒤˈᣇ䲔㥹ࡲⲴ䖜สഐ֌⢙˄ѫ㾱वᤜབྷ 䉶ǃ⦹㊣ǃ⋩㨌ǃỹ㣡ǃ⭌㨌઼㤌㬯˅⿽Ἵ䶒〟Ѫ 1.02 ӯޜ产ˈঐޘ⨳䖜สഐ ֌⢙⿽Ἵ䶒〟Ⲵ 57ˁǄ༽ਸᙗ⣦ӗ૱ᱟ 2010 ᒤ̚2013 ᒤᵏ䰤໎䮯ᴰᘛⲴа㊫ᙗ ⣦ˈ2014 ᒤਜ਼ᴹє⿽ᡆԕкᙗ⣦Ⲵ༽ਸᙗ⣦䖜สഐ֌⢙⿽Ἵ䶒〟䗮ࡠ 5140 зޜ 产ˈঐᙫ䖜สഐ֌⢙⿽Ἵ䶒〟Ⲵ 28%ˈ∄ 2013 ᒤ໎࣐ 9%ˈᐢ㓿䘌䘌䎵ࠪҶঅ аⲴ Bt ᣇ㲛ᙗ⣦ӗ૱˄2014 ᒤ⿽Ἵ䶒〟 2740 зޜ产ˈঐ 15%˅Ǆ2014 ᒤ᣹б 㖾⍢Ⲵᐤ㾯ǃ䱯ṩᔧǃᐤ᣹൝઼Ѽ᣹൝䱶㔝ᔰ࿻⿽Ἵᣇ㲛㙀䲔㥹ࡲⲴ༽ਸᙗ⣦བྷ 䉶ˈᖃᒤ⿽Ἵ䶒〟ণ䗮 580 зޜ产Ǆ༽ਸᙗ⣦ᰕ⳺ᡀѪ䖜สഐ֌⢙Ⲵањ䟽㾱䀂㢢˄James, 2014˅Ǆ 䖜สഐ⦹㊣ᱟӵ⅑Ҿབྷ䉶ⲴㅜҼབྷ䖜สഐ֌⢙ˈ2013 ᒤ⿽Ἵ䶒〟䎵䗷 5740 зޜ产ˈঐޘ⨳䖜สഐ֌⢙⿽Ἵ䶒〟Ⲵ 33%Ǆަѝˈᣇ䲔㥹ࡲ઼ᣇ㲛༽ਸᙗ⣦⦹ ㊣Ⲵ䶒〟ᴰབྷˈѪ 3730 зޜ产ˈঐޘ䜘䖜สഐ֌⢙ᓄ⭘䶒〟Ⲵ 23%˗অ㓟ᣇ䲔㥹ࡲⲴ⦹㊣䶒〟Ѫ 770 зޜ产ˈঐ 5%˗অ㓟ᣇ㲛⦹㊣䶒〟Ѫ 600 зޜ产ˈঐ 4%Ǆ 2013 ᒤˈޘ⨳ᴹ 17 њഭᇦᢩ߶୶ъॆ⿽Ἵ䖜สഐ⦹㊣ˈަѝᧂ਽ࡽ 5 սⲴഭᇦ 䖜สഐ⦹㊣⿽Ἵ䶒〟䎵䗷 100 зޜ产ˈ࠶࡛Ѫ˖㖾ഭ˄3560 зޜ产˅ǃᐤ㾯˄1290 зޜ产˅ǃ䱯ṩᔧ˄320 з ޜ 产 ˅ǃই 䶎˄ 240 зޜ产˅઼࣐᤯བྷ˄170 зޜ产˅Ǆ ⅗ⴏⲴ 5 њᡀઈഭ൘ 2013 ᒤ㔗㔝 Bt 䖜สഐ⦹㊣Ⲵ⿽Ἵˈᙫ䶒〟䗮ࡠ 148,013 ޜ 产ˈ∄ 2012 ᒤ໎䮯㓖 15%Ǆ Klumper ઼ Qaim˄2014˅࡙⭘൘ц⭼਴ൠ䘋㹼Ⲵߌ൪䈳ḕᡆ⭠䰤䈅傼ᗇࠪ Ⲵ৏࿻ᮠᦞሩ䗷৫ 20 ᒤޘ⨳ 147 亩ᐢ⸕Ⲵ䖜สഐ֌⢙⹄ウ䘋㹼Ҷѕ䉘Ⲵ㔬ਸ࠶ ᷀ˈᒦᣕ੺Ҷ䖜สഐབྷ䉶ǃ⦹㊣઼ỹ㣡ሩ֌⢙ӗ䟿ǃߌ㦟֯⭘઼ߌ≁࡙⏖ᯩ䶒Ⲵ ᖡ૽Ǆ䈕㔬ਸ࠶᷀Ⲵ㔃䇪ᱟĀ䖜สഐ֌⢙൘ 1995 ᒤ㠣 2014 ᒤ䰤ӗ⭏Ҷཊ䟽䟽བྷ ˈ᭸⳺˖䟷⭘䖜สഐᢰᵟ֯ॆᆖߌ㦟Ⲵ֯⭘⦷䱽վҶ 37ˁˈ֌⢙ӗ䟿ᨀ儈Ҷ 22ˁ ߌ≁࡙⏖໎࣐Ҷ 68ˁǄᣇ㲛֌⢙Ⲵӗ䟿໎⳺઼ߌ㦟߿ቁⲴ〻ᓖབྷҾᣇ䲔㥹ࡲ֌ ⢙Ǆਁኅѝഭᇦ∄ਁ䗮ഭᇦⲴӗ䟿઼࡙⏖໎⳺儈Ǆā䈕㔃䇪ঠ䇱ҶԕᖰⲴޘ⨳ᒤ ᓖ⹄ウᗇࠪⲴа㠤ᙗ㔃᷌˖Ӿ 1996 ᒤࡠ 2013 ᒤ䰤֌⢙ӗ䟿໎࣐Ⲵՠ㇇ԧ٬Ѫ

182 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ӯ㖾ݳˈަѝˈਁ䗮ഭᇦ㧧ᗇⲴ㍟䇑㓿⍾᭸⳺㓖Ѫ 652 ӯ㖾ݳˈਁኅѝഭ 1333 ᇦӗ⭏Ⲵ㓿⍾᭸⳺㓖Ѫ 681 ӯ㖾ݳ˄Brookes and Barfoot, 2015˅Ǆ MON 87427 ᱟㅜањ㓴㓷⢩ᙗ㺘䗮ᣇ㥹⭈㟖Ⲵ⦹㊣ˈਟԕ㻛⭘ᶕ䇔Ѫࡋ䙐䳴ᙗн㛢Ǆഐ↔ਟԕ⭘Ҿ⦹㊣ᵲӔ⿽ࡦ⿽ǄMON 87427 ᴰᰙҾ 2012 ᒤ൘࣐᤯བྷ 㧧߶୶ъॆ⿽Ἵ䇨ਟˈ䲿ਾ൘㖾ഭҏ㧧ᗇҶ⿽Ἵᢩ߶ǄMON 87427 ሶՊ俆ݸ൘ 㖾ഭ઼࣐᤯བྷ⭘Ҿ୶ъॆᵲӔ⿽ࡦ⿽Ǆ 䖜 cp4 epsps สഐ㙀䲔㥹ࡲ⦹㊣ MON 87427 ᴰᰙҾ 2012 ᒤ൘࣐᤯བྷ㧧߶୶ ъॆ⿽Ἵ䇨ਟˈ䲿ਾ൘㖾ഭҏ㧧ᗇҶ⿽Ἵᢩ߶ˈᒦݸਾ൘໘㾯କǃ◣བྷ࡙ӊ/ᯠ 㾯ޠǃਠ⒮ǃᰕᵜǃ丙ഭǃକՖ∄ӊǃ㨢ᖻᇮǃ䎺ইǃ⅗ⴏㅹഭᇦ઼ൠ४㧧ᗇ䘋 ਓᢩ߶⭘Ҿ伏⭘઼侢⭘Ǆާփᇑᢩᛵߥ䈧㿱л㺘ˈਾ䱴䘋ਓഭᢩ߶᮷Ԧ৏᮷৺㘫䈁Ǆӗൠഭᢩ߶᮷Ԧ৏᮷৺㘫䈁䈧㿱ㅜ 271 亥Ā12. 䖜สഐ⭏⢙൘䗃ࠪഭᇦᡆൠ ४ᐢ㓿ݱ䇨֌Ѫ⴨ᓄ⭘䙄ᒦᣅ᭮ᐲ൪Ⲵ䇱᰾᮷ԦāǄ

183 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䖜 cp4 epsps สഐ㙀䲔㥹ࡲ⦹㊣ MON 87427 ⴞࡽ൘ޘ⨳㤳ത޵Ⲵᢩ߶ᛵߥ

᢯߼൦॰ ᇗ᢯ᵰᶺ ᢯߼⭞䙊 ᢯߼ᒪԳ

ࣖᤵཝ ࣖᤵཝড⭕䜞δHCε伕⭞2012 ᒪ ࣖᤵཝ ࣖᤵཝ伕૷Ỷ⯡ቶδCFIAε侨⭞/〃ὃ 2012 ᒪ 㗄ള 㗄ള伕૷ф㦥૷ⴇ⶙㇗⨼ቶδFDAε 伕⭞/侨⭞ 2012 ᒪ 㗄ള 㗄ളߒѐ䜞ࣞὃ⢟Ỷ⯡ቶδUSDA- APHISε 〃ὃ 2013 ᒪ ໞ㾵ଛড⭕䜞δMOHε – ໞ㾵ଛ 伕⭞ 2012 ᒪ 㚊䛜ড⭕伄䲟人䱨㚊䛜ည઎ՐδCOFEPRISε ◩ཝ࡟Ӑ/ ◩᯦伕૷ḽ߼㇗⨼ቶδFSANZε伕⭞2012 ᒪ ᯦㾵ަ ਦ⒴ ਦ⒴㺂᭵䲘ড⭕㖨δDOHε伕⭞2012 ᒪ ᰛᵢ ᰛᵢ৐⭕ࣩࣞⴷδMHLWε 伕⭞ 2013 ᒪ ᰛᵢ ᰛᵢߒ᷍≪ӝⴷδMAFFε 侨⭞ 2013 ᒪ ᰛᵢߒ᷍≪ӝⴷδMAFFε- ᰛᵢ ⧥ູ 2013 ᒪ ᰛᵢ⧥ؓ㖨δMOEε 丟ള 丟ളߒᶇਇኋ㇗⨼ቶδRDAε 侨⭞ 2013 ᒪ 丟ള 丟ള伕૷㦥૷㇗⨼ቶδMFDS) 伕⭞ 2014 ᒪ ଛ՜∊Ӑളᇬড⭕䜞δCTN-Healthε- ଛ՜∊Ӑ 伕⭞ 2013 ᒪ 伕૷㦥૷ⴇ⶙ቶδINVIMAε ଛ՜∊Ӑളᇬ⭕⢟ᢶᵥည઎ՐδCTNbioε- ଛ՜∊Ӑ 侨⭞ 2014 ᒪ ߒѐ⹊ガᡶδICAε 㨨ᗁᇴ 㨨ᗁᇴߒѐ䜞ὃ⢟ӝѐቶδBPIε 伕⭞/侨⭞ 2014 ᒪ 䏀঍ 䏀঍ߒѐૂߒᶇਇኋ䜞δMARDε 伕⭞/侨⭞ 2015 ᒪ ⅝ⴕ ⅝ⴕည઎ՐδECε 伕⭞/侨⭞ 2015 ᒪ ᯦ࣖ඗ AVA 伕⭞/侨⭞ 2016 ᒪ

184 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 1Ƚໞ㾵ଛড⭕䜞᢯߼ᮽԬ δMOHθ伕⭞ε

185 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ড⭕䜞㚊䛜ড⭕伄䲟人䱨ည઎Ր ড⭕᢯߼ည઎Ր

䖢ะഖ⭕⢟୼ѐौૂ䘑ਙⲺড⭕᢯߼ 㕌ਭφ123300913X0002

ᦤφໞ㾵ଛਾՍള᭵⋱ᇠ⌋ㅢ 4 ᶗㅢ 3Ƚ8Ƚ14 ૂ 16 亯θ㚊䛜㺂᭵ぁᓅד ⌋Ṿㅢ 1Ƚ3Ƚ16-X 亯θໞ㾵ଛ䙐⭞ড⭕⌋ㅢ 3-XXIV ᶗȽㅢ 4-III-17 ᶗȽㅢ 4-IV ᶗȽㅢ 194-I-282Ƚ194-I-282-1Ƚ194-I-282-2 ᶗȽㅢ 368 ᶗȽㅢ 371 ᶗθড⭕ 䜞޻䜞⌋㿺ㅢ 2 ㄖㅢ c-X 㢸θ䖢ะഖ⭕⢟ᆿޞ⌋ㅢ 91Ƚ93Ƚ94 ૂ 97 ᶗθ2008 ᒪ 3 ᴾ 19 ᰛ㚊䛜ᇎᯯޢ઀рਇᐹⲺ䖢ะഖ⭕⢟ᆿޞ⌋㿺ㅢ 23Ƚ24Ƚ25 ૂ 28 ᶗθ㚊䛜ড⭕伄䲟人䱨㇗⨼ည઎Ր⌋㿺ㅢ 1 ᶗȽㅢ 3-I-K ૂ 3-IȽ-VȽ-VIIȽ-XI ૂ-XII ᶗȽㅢ 4 ᶗȽㅢ 12-I ᶗૂㅢ 14-I ᶗθ2004 ᒪ 12 ᴾ 28 ᰛ㚊䛜ᇎᯯޢ઀р ਇᐹⲺ᤽ᇐ㚊䛜ড⭕伄䲟人䱨ည઎Ր㇗⨼ᵰᶺ㚂㜳ԙ⨼অ䇤θᒬ㘹㲇ࡦ伄䲟䇷ᦤ ᦤᡶ᭬ࡦⲺ⭩䈭䍺ᯏㆴਇⲺ䇺զᮽԬθ⢯㔏Ҿԛсӝ૷ԛ᢯߼φד㇗⨼ည઎Ր

᢯߼ᤷᴿӰφ Engineer Jose Javier Gándara Espinosa Monsanto Comercial S.A. de C.V. Prolongación Paseo de la Reforma No. 1015 Torre A Piso 21, Colonia Desarrollo Santa Fe Delegación Alvaro Obregón C.P 01376 Mexico D.F.

㚊䛜᭵ᓒ⌞߂ⲱ䇦ਭδR.F.C.ε MCO-701113-C5A

186 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ӝ ૷ ⢯ ᖷ

୼ѐौ੃〦φ RHS ⦿㊩ ᆜ੃φ Zea mays 〇 Familyφ ⿴ᵢ〇 Graminaceous ኔ Genreφ ⦿㴶唃ኔ Zea 〃 Speciesφ ⦿㊩〃 mays ṳฯ〃 Cultivarφ Zea mays OECD 䇼ࡡਭ⸷φ MON-87427-7 䖢ौ։㊱ශφ ߒᵼ㨂ԁሲ䖢ौ ࣕ㜳ᙝ⣬φ 㓺㓽⢯ᔸᙝᣍ㥿⭎㟜䚍Ֆどᇐᙝ どᇐᙝ儎 ᇔ䍞ㅿੂᙝφ фੂශᑮ㿺ሯ➝૷〃ᇔ䍞ㅿੂ ᓊ⭞㤹പφ ࣞ⢟侨⭞ᡌӰ㊱伕⭞Ⲻ䘑ਙૂ୼ѐौ᢯߼ ᢯߼㤹പφ ޞള॰ต ᢯߼⣬ᘷφ ㊳㋈Ƚ㣧਀ࢥӝ૷ 㠪ᮅᙝφ ք ∈ᙝφ ᰖ∈ ৕ӝളφ 㗄ള 亯ⴤᶛⓆളᇬφ 㗄ള 㺞䗴㴁ⲳφ 㓺㓽⢯ᔸᙝ㺞䗴 CP4 EPSPS 㴁ⲳ

㿺ㅢ 37 ૂ 40⌊ޞṾㅢ 69 ૂ 98 ᶗȽ䖢ะഖ⭕⢟ᆿ⌊ޞᦤ䖢ะഖ⭕⢟ᆿד ᶗθؓ⮏㔝㔣ሯр䘦᢯߼䘑㺂ᇗṮૂӝ૷䘑㺂䇺ԭⲺᵹ࡟Ⱦ

᢯߼ᰛᵕφ2012 ᒪ 11 ᴾ 16 ᰛ

ᛞ䈐ᥐⲺ FEDERICO ALBERTO ARGÜELLES TELLO ড⭕伄䲟人䱨ည઎Րщ઎

187 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 188 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 189 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 2Ƚ◩ཝ࡟Ӑ-᯦㾵ަ伕૷ḽ߼㇗⨼ቶ᢯߼ᮽԬ δFSANZθ伕⭞ε

190 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䇘ؤㅢ 133 ਭ

ԛсޢ઀ൽѰɅ㚊䛜㄁⌋ᮽҜޢᣛδFederal Register of Legislative 䇘Ⱦؤ઀ɆѣжᒬᖈѰㅢ 133 ਭޢ઀θ൞Ʌ伕૷ḽ߼ޢInstrumentsεɆѣ⤢㄁

޻ᇯࡍ㺞

伕૷ḽ߼δ⭩䈭ਭφA1064——CV127 ᣍ䲚㥿ࡸཝ䉼ᶛⓆ伕૷ε਎ᴪ 伕૷ḽ߼δ⭩䈭ਭφA1066——MON 87427 ᣍ䲚㥿ࡸ⦿㊩ᶛⓆ伕૷ε਎ᴪ

191 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 伕૷ḽ߼δ⭩䈭ਭφA1066——MON 87427 ᣍ䲚㥿ࡸ⦿㊩ᶛⓆ伕૷ε਎ᴪ

ᦤɅ◩᯦伕૷ḽ߼⌋Ṿδ1991εɆדཝ࡟Ӑ-᯦㾵ަ伕૷ḽ߼㇗⨼ቶ⨼ӁՐ◩ ㅢ 92 ㄖ㿺ᇐθㆴਇԛс਎ᴪ䙐⸛Ⱦḽ߼⭕᭾ᰛᵕ䚫➝ᵢ਎ᴪޢ઀ѣᶗⅴ 3 ޻ᇯȾ

2012 ᒪ 7 ᴾ 9 ᰛ

⌋ᗁф⌋㿺Ӂࣗ䜞θᙱ⨼Ӂ ԙ㺞◩ཝ࡟Ӑ-᯦㾵ަ伕૷ḽ߼㇗⨼ቶ⨼ӁՐ

1 ੃〦 ᵢޢ઀ᱥሯ伕૷ḽ߼δ⭩⭩䈭ਭφA1066——MON 87427 ᣍ䲚㥿ࡸ⦿㊩ᶛⓆ 伕૷εⲺ਎ᴪȾ 2 ሯɅ◩ཝ࡟Ӑ-᯦㾵ަ伕૷ḽ߼⌋ޮɆѣḽ߼Ⲻ਎ᴪ

Ʌ◩ཝ࡟Ӑ-᯦㾵ަ伕૷ḽ߼⌋ޮɆѣḽ߼Ⲻ޻ᇯࡍ㺞ਇ⭕਎ᴪȾ 3 ⭕᭾ᰛᵕ 䈛਎ᴪ㠠 2012 ᒪ 7 ᴾ 12 ᰛ䎭⭕᭾Ⱦ

޻ᇯࡍ㺞

[1] ḽ߼ 1.5.2 ഖ᯦໔ྸсᓅࡍਭ㙂ਇ⭕਎ᴪφ

2.21 MON 87427 ᣍ䲚㥿ࡸ⦿㊩ᶛⓆ伕૷

δ䈇⌞φCV127 ѰެԌޢਮཝ䉼ӝ૷θ㘱䈇⮛ε

192 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 193 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 194 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 195 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 196 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 197 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 3Ƚਦ⒴㺂᭵䲘ড⭕㖨᢯߼ᮽԬ δDOHθ伕⭞ε

198 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 199 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 200 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 4Ƚᰛᵢ৐⭕ࣩࣞⴷ᢯߼ᮽԬ δMHLWθ伕⭞ε

201 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᰛᵢ৐⭕ࣩࣞⴷδMHLWεޢ઀ㅢ 162 ਭ 䢪ӄԛс࡟⭞ DNA 䠃㓺ᢶᵥӝ⭕Ⲻ⭕⢟ᐨ㔅䙐䗽伕૷ф伕૷␱ࣖࡸㅢ 1A-2 ਭḽ߼δ1959 ᒪ৐⭕ⴷޢ઀ㅢ 370 ਭεᇗḛぁᓅθཝ㠙↙ᕅᇙᐹԛс⭕⢟ᐨ㔅 ᤿➝䠃㓺 DNA ᢶᵥᶛⓆⲺ伕૷ૂ伕૷␱ࣖࡸᆿޞ䇺ԭぁᓅㅢ 3Њ4 ਭ㿺ᇐδ2000 ᒪ MHLW ޢ઀ㅢ 233 ਭεθᇂᡆҼᆿޞᙝ䇺ԭȾ

2013 ᒪ 5 ᴾ 2 ᰛ Norihisa Tamura ৐⭕ࣩࣞⴷཝ㠙

䙐䗽Ҽ伕૷ૂ伕૷␱ࣖࡸ䇺ԭぁᓅⲺ䠃㓺 DNA ᢶᵥᶛⓆⲺ⭕⢟φ ӝ૷ ૷㌱/䖢ौ։੃〦 ⭩䈭Ӱ/⢟֒ 㥿⭎㟜䈧ሲ䳺ᙝу㛨ૂ㙆㥿⭎㟜⦿ ⦿㊩ ᆕኧ䜳ᰛᵢᴿ䲆ޢਮ ㊩ MON 87427

202 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 203 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 5Ƚᰛᵢߒ᷍≪ӝⴷ᢯߼ᮽԬ δMAFFθ侨⭞ε

204 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䠃㓺 DNA ᢶᵥᶛⓆⲺ侨ᯏᇂᡆᆿޞᙝ⺤䇚δᇎᯯޢ઀ε ᦤ侨ᯏૂ侨ᯏ␱ࣖࡸᡆ࠼ḽ߼Ⲻ䜞㓝ᶗׁδ1976 ᒪߒ᷍≪ӝ䜞㓝ᶗד䢪ӄ ㅢ 35 ਭε䱺Ԭ 1-1-(1)Ⲻ㾷≸θߒ᷍≪ӝⴷδMAFFε⺤䇚Ҽԛс࡟⭞䠃㓺 DNAׁ ᢶᵥ⭕ӝⲺ侨ᯏⲺ侨⭞ᆿޞᙝθᵢ䜞䮵ਇ㺞ᇎᯯޢ઀φ᤿➝䠃㓺 DNA ᢶᵥӝ⭕ Ⲻ侨ᯏૂ侨ᯏ␱ࣖࡸⲺᆿޞぁᓅδ2002 ᒪ 11 ᴾ 26 ᰛߒ᷍≪ӝⴷㅢ 1780 ਭޢ ઀εㅢ 4 ⅴ㿺ᇐⲺ㾷≸θԛс侨⭞ᆿޞᙝᗍࡦ⺤䇚Ⱦ

2013 ᒪ 5 ᴾ 2 ᰛ Yoshimasa Hayashi ߒ᷍≪ӝⴷཝ㠙

ᇂᡆҼᆿޞᙝ⺤䇚Ⲻ䠃㓺 DNA ᢶᵥᶛⓆⲺ侨ᯏφ ӝ૷ ૷㌱/䖢ौ։੃〦 ⭩䈭Ӱ/⢟֒ ਮޢ㊩ ᣍ䷎㗻ⴤᇩ㲡⦿㊩䖢ौ։ 5307 ݾ↙䗴ᰛᵢᴿ䲆⦿ 㥿⭎㟜䈧ሲ䳺ᙝу㛨ૂ㙆㥿⭎㟜⦿ ⦿㊩ ᆕኧ䜳ᰛᵢᴿ䲆ޢਮ ㊩ MON 87427

205 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 206 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 6Ƚᰛᵢߒ᷍≪ӝⴷ-ᰛᵢ⧥ؓ㖨᢯߼ᮽԬ δMAFF / MOEθ⧥ູε

207 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᭬ԬӰφSeiichiro Yamane ᆕኧ䜳ᰛᵢᴿ䲆ޢਮθ⌋Ӱԙ㺞޲ᢝ㺂ᙱ㻷 ᇺԬӰφYoshimasa Hayashi ߒ᷍≪ӝⴷཝ㠙 Nobuteru Ishihara ⧥ູⴷཝ㠙

ഔགྷφሯ㊱ශ I ⭞䙊Ⲻ⌋㿺᢯߼ ᦤ䙐䗽฼┗⍱ᙝ᭯䙖⭕⢟։ᓊ⭞ሯ⭕⢟ཐṭᙝ䘑㺂ؓᣚфਥᤷ㔣ᓊ⭞Ⲻ⌋ᗁד

ਮṯᦤ䙐䗽㿺㤹⍱ᙝ᭯䙖⭕⢟։ޢߒ᷍≪ӝⴷཝ㠙ૂ⧥ູⴷཝ㠙䙐⸛ᛞθ֖ ᓊ⭞ሯ⭕⢟ཐṭᙝ䘑㺂ؓᣚфਥᤷ㔣ᓊ⭞⌋δ2003 ᒪㅢ 97 ਭ⌋ᗁεㅢ 4 ᶗㅢ 1 䜞࠼ӄ 2011 ᒪ 6 ᴾ 17 ᰛᨆӚⲺ⭩䈭θྸ䱺Ԭ㺞Ṳᡶࡍθᐨ㔅㧭ᗍ㊱ශ 1 ⭞䙊 ᢯߼δ1 亯εȾ

208 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 δ䱺㺞ε ᢯߼㕌ਭ: 13-46P-0025 㧭ᗍ᢯߼ޢਮθ⌋Ӱ ᆕኧ䜳ᰛᵢᴿ䲆ޢਮ ԙ㺞ဉ੃фࣔޢᇚ൦ Seiichiro Yamaneθᙱ㻷 ൶ 4-10-10 Ginza, Chuo-ku, Tokyo ᢯߼㊱ශ 1 ⭞䙊侦Ⲻؤ⍱ᙝ᭯䙖⭕⢟։੃〦㥿⭎㟜䈧ሲ䳺ᙝу㛨ૂ㙆㥿⭎㟜⦿㊩δ㔅䗽 cp4 epspsθZea mays subsp. mays (L.) IltisεδMON87427, OECD UIφMON-87427-7ε ⍱ᙝ᭯㢥⭕⢟։㊱ශ ᶛ㠠ӄ⍱ᙝ᭯㢥⭕⢟։Ⲻ伕૷ᡌ侨ᯏⲺᓊ⭞ȽṳฯȽࣖᐛȽ ⭞䙊Ⲻ޻ᇯ ۞ᆎȽ䖢〱ૂ༺⨼θԛ਀ެᆹ⴮ީ㺂ѰȾ 1 ⍱ᙝ᭯㢥⭕⢟։㊱ශ _ 1 ⭞䙊ᯯᕅ

209 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 210 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 211 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 7Ƚ丟ളߒᶇਇኋ㇗⨼ቶ᢯߼ᮽԬ δRDAθ侨⭞ε

212 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߒᶇਇኋ㇗⨼ቶ

᧛᭬ঋփྸс

从ⴤφߒѐᓊ⭞⭕⢟ᢶᵥ᭯㢥⭕⢟ MON 87427 ⧥ູ伄䲟䇺ԭᇗḛ㔉᷒ޢ઀

ᦤɅ⍱ᙝ᭯㢥⭕⢟։ㅿ䏀ູ䖢〱⌋Ɇㅢ 16 ᶗⅴδ伄䲟䇺զ૞䈘εדഔགྷφ .1 ૂㅢ 13 ᶗⅴδ伄䲟䇺զ਀ညᢎᇔ᯳⍷ぁεθԛ਀Ʌ䪾ሯ⍱ᙝ᭯㢥⭕⢟։ㅿ 䏀ູ䖢〱Ⲻ㔕жޢ઀Ɇㅢ 1-4 ᶗδԙ㺞/ㆴ㓜ᵰᶺεㅢж⇫ૂ 4-8 ᶗδ⧥ ູ伄䲟䇺զㅿεȾ

ਮᨆӚⲺ⍱ᙝ䚍Ֆ᭯㢥⦿㊩ޢṯᦤԛрؗᚥθᡇԢ䙐઀䱺Ԭѣᆕኧ䜳丟ള .2 δMON 87427εߒѐᓊ⭞Ⲻᇗḛ㔉᷒Ⱦ

a. ᇗḛ亯ⴤφ䚍Ֆ᭯㢥ᣍ䲚㥿ࡸδ㓺㓽䘿᤟ᙝε⦿㊩δMON 87427ε b. ⭩䈭Ӱφᆕኧ䜳丟ളޢਮ c. ⭩䈭ᰛᵕφ2011 ᒪ 6 ᴾ 28 ᰛ

䱺Ԭφ䚍Ֆ᭯㢥⍱ᙝ⭕⢟։ߒѐᓊ⭞⧥ູ伄䲟ᇗḛ㔉᷒δNo 2013-07εȾ㔉ᶕȾ

ߒᶇਇኋ㇗⨼ቶቶ䮵

᧛᭬Ӱφ伕૷ȽߒѐȽ᷍ѐૂ⑊ѐ䜞䜞䮵δḽ䇼ȽỶ⯡ૂⴇ⶙༺༺䮵εȽളᇬߒ ѐӝ૷䍞䠅㇗⨼ᵃࣗ䜞δ৕ӝ൦Ỷ⎁ഘ䱕䍕䍙ӰεȽᆕኧ䜳丟ളޢਮ

213 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䚍Ֆ᭯㢥⍱ᙝ⭕⢟։ߒѐᓊ⭞⧥ູ伄䲟ᇗḛ㔉᷒

ᦤφדᗁ⌊

Ʌ⍱ᙝ᭯㢥⭕⢟։ㅿ䏀ູ䖢〱⌋Ɇㅢ 16 ᶗⅴδ伄䲟䇺զ૞䈘εૂㅢ 13 ᶗⅴδ伄䲟䇺զ਀ညᢎᇔ᯳⍷ぁεχ Ʌ䪾ሯ⍱ᙝ᭯㢥⭕⢟։ㅿ䏀ູ䖢〱Ⲻ㔕жޢ઀Ɇㅢ 1-4 ᶗδԙ㺞/ㆴ㓜ᵰᶺεㅢ ж⇫ૂ 4-8 ᶗδ⧥ູ伄䲟䇺զㅿεȾ

伄䲟ᇗḛ޻ᇯφ

⭧ӄɅ⍱։᭯㢥⭕⢟։ㅿ䏀ູ䖢〱⌋Ɇӄ 2008 ᒪ 1 ᴾ 1 ᰛᔶခᇔ᯳θ䘑ਙᡌ⭕ ӝ䖢ะഖ⭕⢟䴶㾷㔅䗽伄䲟ᇗḛχ

ᣍ䲚㥿ࡸ⦿㊩ MON 87427 ⧥ູ伄䲟ᇗḛ⭩䈭ӄ 2011 ᒪ 6 ᴾ 28 ᰛᨆӚθ㔅䗽щ ᇬ䇺ᇗည઎ՐⲺ 3 䖤ᇗḛȾ

ᇗḛ㔉᷒

ᣍ䲚㥿ࡸ⦿㊩ MON 87427

㊱ශ ӝ૷੃〦 ⭩䈭Ӱ ⢯ᙝ ᨆӚᰬ䰪 ᇗḛᇂᡆᰬ䰪 ᆕኧ䜳丟ള 2011 ᒪ 2013 ᒪ ⦿㊩ MON 87427 ᣍ䲚㥿ࡸ ޢਮ 6 ᴾ 28 ᰛ 12 ᴾ 16 ᰛ

ṯᦤㅢ 101 ⅗⧥ູ伄䲟щᇬ䇺ᇗည઎ՐՐ䇤δ2013 ᒪ 12 ᴾ 13 ᰛεⲺᇗḛ㔉᷒θ ߒᶇਇኋ㇗⨼ቶቶ䮵⺤䇚൞䘑ਙ⭞֒侨ᯏᡌ侨ᯏࣖᐛ㙂䶔ṳฯⲺ䗽ぁѣθྸ᷒ਇ ⭕ᰖᝅ⍷ᮙθ䚍Ֆ᭯㢥⦿㊩ MON 87427 ߒѐᓊ⭞ሯള޻ߒѐ⧥ູуՐӝ⭕伄䲟Ⱦ

䱺Ԭφ䚍Ֆ᭯㢥⍱ᙝ⭕⢟։ߒѐᓊ⭞⧥ູ伄䲟ᇗḛ㔉᷒

214 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䙐⸛㕌ਭ ߒѐ⍱ᙝ᭯㢥⭕⢟⧥ູ伄䲟ᇗḛ㔉᷒ 2013-07

ޢਮ ᆕኧ䜳丟ളޢਮ ޢਮ⌞߂ਭ⸷ 214-81-04623

5th floor, Gwang-Hui building, 216 Gwanghuidong 1-ga, ⭩䈭Ӱ ൦൶ Jung-gu, Seoul Benjamin Mark ਮԙ㺞㚊㌱ؗᚥ 02-3393-3700ޢ Hoodless ੃〦 ᆕኧ䜳ޢਮ ⹊ਇঋփ ൦൶ 800 North Lindbergh Boulevard, St. Louis, MO 63167, USA

䙐⭞੃ ᆜ੃ ૷〃/૷㌱ ੃〦 ⦿㊩ Zea mays L. MON 87427 ⭞䙊侨ᯏᡌ侨ᯏࣖᐛ

ᇗḛ㔉᷒ ᢯߼δOε

᢯߼㤹പ ӻ᢯߼⭞֒侨ᯏᡌ侨ᯏࣖᐛ

઀Ɇㅢ4Њ8ᶗⅴㅢ6⇫㩳⴮ީ⌋㿺ቧޢᦤɅᴿީ⍱ᙝ᭯㢥⭕⢟։ㅿ䏀ູ䖢〱Ⲻ㔕жד р䘦⭩䈭Ⲻᇗḛ㔉᷒Ҿԛ䙐⸛Ⱦ

2013ᒪ12ᴾ20ᰛ

ߒᶇਇኋ㇗⨼ቶቶ䮵

⧥ູ伄䲟ᇗḛ㔉᷒ᣛ઀䱺੄

ᦤ⭩䈭㘻ᨆӚⲺᮽԬ䍺ᯏᗍ࠰р䘦ᇗḛ㔉䇰θ⭩䈭㘻䴶㾷ሯᨆӚⲺᮽԬ䍺ᯏҁཌד ᙝⲺ᯦Ⲻؗᚥ䴶㾷ታᘡᣛ઀ߒᶇਇኋޞⲺެᆹ⴮ީؗᚥ䍕䍙θྸ᷒ਇ⧦Ա֋ᴿީ⧥ູᆿ ㇗⨼ቶቶ䮵Ⱦ

215 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 216 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 217 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 218 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 219 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 8Ƚ丟ള伕૷㦥૷㇗⨼ቶ᢯߼ᮽԬ δMFDSθ伕⭞ε

220 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 伕૷ф㦥૷ᆿޞቶ

᭬ԬӰφᆕኧ䜳丟ളᴿ䲆ޢਮ ḽ从φ䚍Ֆ᭯㢥⦿㊩ MON 87427 ᆿޞᙝ䇺ԭ䍺ᯏᇗḛ㔉᷒䙐઀

1. 㕌ਭ 20110176816δ2011.6.30ε⭩䈭Ⲻഔགྷ

2. ީӄр䘦⭩䈭θṯᦤć䚍Ֆ᭯㢥伕૷ᆿޞᙝ䇺ԭᇗḛ᤽঍Ĉθሯ⭩䈭㘻ᨆ ӚⲺ䚍Ֆ᭯㢥⦿㊩ MON 87427 ᐨ㔅ᇂᡆᇗḛθഖ↚ᵢቶ֒࠰ᇗḛ㔉䇰θ ᇗḛ㔉᷒䙐⸛䱺੄Ⱦ

ᦤ⭩䈭㘻ᨆӚⲺᮽԬ䍺ᯏᗍ࠰Ⲻθ⭩䈭㘻ሯᨆӚⲺᮽԬ䍺דᇗḛ㔉䇰ᱥ .3 䍙θഖ↚ᵢ㇗⨼ቶ㾷≸⭩䈭Ӱ㔝㔣㇗⨼↚䖢ौ։θྸޞᯏҁཌⲺؗᚥ䍕 ᙝⲺ᯦Ⲻؗᚥ䴶㾷ታᘡᣛ઀㇗⨼ቶȾޞ᷒ਇ⧦Ա֋ᴿީᆿ

䱺Ԭφ 1. 䚍Ֆ᭯㢥⦿㊩ MON 87427 ᆿޞ䇺ԭᇗḛ㔉᷒䙐઀ 2. 䚍Ֆ᭯㢥⦿㊩ MON 87427 ᆿޞᙝ䇺ԭᇗḛ㔉᷒

丟ള伕૷㦥૷㇗⨼ቶщ઎

ᇗḛ㘻φSi-Young Lee 䍺␧ᇗḛ઎φYong-Kwan Kwan 䍕䍙Ӱ

11/23

221 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䙐⸛㕌ਭ ⭕⢟ᢶᵥ᭯㢥伕૷ᆿޞᙝ䇺ԭᇗḛ㔉᷒䙐⸛ 2014-2 ޢਮ ᆕኧ䜳丟ളޢਮޢਮ⌞߂㕌ਭ 214-81-04623 ൦൶ 12th floor, S Tower, 82 Saemunan-Ro, Jongro-gu, Seoul ⭩䈭Ӱ Hoodless ⌋Ӱԙ㺞㚊㌱ᯯᕅ 02-3393-3768 Benjamin Mark ੃〦 ᆕኧ䜳ޢਮ ⹊ਇঋփ ൦൶ 800 N Lindbergh Boulevard, St. Louis, MO 63167, USA ㊱ࡡ ㅢ3ⅴㅢ1⇫ Ţ⭕⢟ ⦿㊩ ţ䖢ौ։/૷㌱ MON 87427 Ο୼ḽ੃〦 - Πᕋޛᙝ⣬ ᣍ䲚㥿ࡸ Ρᨈޛะഖ cp4 epsps ΢᢯߼рᐸⲺㅢжѠളᇬૂ USA δ2012ε ᢯߼ᰬ䰪 Σ᢯߼рᐸⲺެԌളᇬૂ᢯ ◩ཝ࡟Ӑૂ᯦㾵ަδ2012εȽࣖᤵཝδ2012εȽໞ㾵 ߼ᰬ䰪 ଛδ2012εȽѣളਦ⒴δ2012εȽᰛᵢδ2013ε Τ䘑㺂ᆿޞᙝ䇺ԭⲺެᆹള ⅝ⴕδ2011ε ᇬૂ⭩䈭ᮽԬᖈẙᰛᵕ ᯦ӝ૷δOεχᐨ㔅୼ѐौ10ᒪԛрδ εχ Υӝ૷⣬ᘷ㊱ࡡ ᐨ୼ѐौӝ૷䘶ᐸδ ε Φᇗḛ㔉᷒ ᢯߼ δOεχ ᴿᶗԬ᢯߼δ εχ у䘸⭞δ ε Χᇗ᢯ⴤⲺδ伕⭞ε 䘑ਙδOεχӝ૷ᔶਇδ εχ⭕ӝδ εχެᆹδ ε

ᡇԢ൞↚䙐⸛ᛞθ᤿➝Ʌ⭕⢟ᢶᵥ伕૷ᆿޞᙝ䇺ԭᇗḛ᤽঍Ɇㅢ9ᶗⲺ㾷≸ሯ⭕⢟ᢶᵥ ᭯㢥伕૷Ⲻᆿޞᙝ䇺ԭᇗḛ㔉᷒Ⱦ 2014ᒪ1ᴾ9ᰛ 丟ള伕૷㦥૷㇗⨼ቶщ઎ ㆴᆍ

䱺ԬᮽԬѰᆿޞᙝ䇺ԭᇗḛ㔉᷒ᣛ઀གྷদԬȾ

ᦤ⭩䈭㘻ᨆӚⲺᮽԬ䍺ᯏᗍ࠰Ⲻθ⭩䈭㘻䴶㾷ሯᨆӚᮦᦤҁཌⲺؗᚥ䍕דᇗḛ㔉䇰ᱥ ቶȾޞᙝⲺ᯦Ⲻؗᚥθ䴶㾷㄁঩ᣛ઀伕૷ф㦥૷ᆿޞ䍙θྸ᷒ਇ⧦Ա֋ᴿީᆿ

222 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 223 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 224 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 9Ƚଛ՜∊Ӑളᇬড⭕䜞-伕૷㦥૷ⴇ⶙ቶ᢯߼ᮽԬ δCTN-Health / INVIMAθ伕⭞ε

225 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ଛ՜∊Ӑড⭕ф⽴Րؓ䳒䜞 ߩ䇤㕌ਭφ2014 ᒪㅢ 00001862 ਭ 2014 ᒪ 5 ᴾ 20 ᰛ

᢯߼ MON 87427δMON-87427-7ε⦿㊩֒ѰӰ㊱伕૷⎾䍯ᡌ伕૷ࣖᐛ৕ᯏֵ⭞

ଛ՜∊Ӑড⭕ф⽴Րؓ䳒䜞 ࡟⭞ެ⌋ᗁᵹ࣑θ⢯ࡡᱥ 2005 ᒪ 4525 ⌋Ԛㅢ 6 ⅴᡶ䎁ҾⲺ⌋ᗁᵹ࣑ԛ਀φ

䢪ӄφ

㚊ਾളީӄ⭕⢟ཐṭᙝঅ䇤—Ʌޞ⨹⭕⢟ཐṭᙝ⌋ṾɆӄ 1992 ᒪ 6 ᴾ 5 ᰛᇔ᯳θଛ՜∊Ӑ൞ 1994 ᒪㅢ 165 ⌋Ԛѣ䙐䗽䈛⌋Ṿθᴶ儎ᇠ⌋⌋ᓣ δHornorable Constitutional Courtε䙐䗽 1994 ᒪ C-519 ࡚ߩᇙᐹᴿ᭾χ

⭕⢟ཐṭᙝཝՐҁɅগຊ䎡㓩⭕⢟ᢶᵥᆿޞ䇤ᇐҜɆӄ 2000 ᒪ 1 ᴾ 29 ᰛ 㧭ᗍ᢯߼θଛ՜∊Ӑ൞ 2002 ᒪㅢ 740 ⌋Ԛѣ᢯߼䈛䇤ᇐҜχᴶ儎ᇠ⌋⌋ᓣ δHornorable Constitutional Courtε䙐䗽 2003 ᒪ C-071 ࡚ߩᇙᐹᴿ᭾χ

⌊ ᦤ 2002 ᒪ 740 ⌋Ԛᡶ㿺ᇐⲺぁᓅθളᇬ᭵ᓒᵰᶺ䙐䗽 2005 ᒪ 4525ד Ԛᔰ㄁Ҽᴿީ䚍Ֆ᭯㢥⭕⢟δGMOεⲺ⌋㿺ṼᷬȾ

ᢶᵥည઎ՐδCTNSaludεㆴ㖨䙐䗽Ⲻ 2007 ᒪޞ⭧⽴Րؓ䳒䜞ളᇬ⭕⢟ᆿ ਭߩ䇤ㅢ 1 ⅴθCTNSalud ᡆ઎ऻᤢ⽴Րؓ䳒䜞䜞䮵ᡌެԙ㺞Ƚളᇬ㦥૷ૂ 227 伕૷ⴇ⶙ቶδINVIMAεቶ䮵ᡌެԙ㺞ԛ਀〇ᆜᢶᵥ䜞δCOLCINECIASε䜞䮵ᡌ ެԙ㺞Ⱦ

⢟ࢃ䘦ည઎ՐⲺ㚂㜳ҁжᱥᔰ䇤ড⭕ф⽴Րؓ䳒䜞ㆴ㖨ᴿީ䚍Ֆ᭯㢥⭕ δGMOε⍱ࣞⲺ㺂᭵ᇗ᢯Ⱦ

ߒѐㆶौ㛗Գᴿ䲆ޢਮδCOMPANIA AGRICOLA S.A.Sεδޢਮ൦൶φBogota D.Cε⌋㿺Ӂࣗᙱⴇ Manuel Rivas ঐ༡фളᇬ㦥૷ૂ伕૷ⴇ⶙ቶδINVIMAε䘑㺂Ҽ⋕䙐θ䈭≸ളᇬ⭕⢟ᢶᵥᆿޞည઎Րቧ㓺㓽⢯ᔸᙝᣍ㥿⭎㟜⦿㊩ MON 87427 ⭞ӄӰ㊱伕૷⎾䍯䘑㺂䇺զȽ⹊ガθᒬᨆ࠰ᔰ䇤ȾINVIMA 䍺ᯏⲱ䇦㕌ਭφ2011 ᒪ 12 ᴾ 9 ᰛㅢ 1118151 ਭȾ

226 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ളᇬ⭕⢟ᆿޞᢶᵥည઎ՐδCTNSaludεӄ 2013 ᒪ 5 ᴾ 6 ᰛδՐ䇤䇦ᖋ㕌 ਮᨆӚⲺ᭥ᤷ MON 87427δMON-87427-7ε⦿㊩⭞֒伕૷⴪᧛ޢਭφ2εቧр䘦䍯ᡌࣖᐛ৕ᯏᡶᨆӚⲺ伄䲟ૂᰖᇩⲺ䇺ԭؗᚥ䘑㺂Ҽ࠼᷆θ㔉䇰ྸсφ⎾

aεMON 87427 䖢ौ։ᱥ࡟⭞ PV-ZMAP1043 䍞㋈䙐䗽ߒᵼ㨂ԁሲ䖢ौ LH198×HiII ⦿㊩૷㌱ᒲ㜐ᗍࡦⲺδP18Aεχ

bεMON 87427 ᕋޛⲺ cp4 epsps 㕌⸷ᓅࡍ㺞䗴ӝ⭕ 5-✥䞽ᕅя䞤䞦㧳 ⢟㥿䞮ਾ䞬δCP4 EPSPSεθ䘏〃䞬фᐨ㔅୼ѐौཐᒪⲺᣍ㥿⭎㟜֒ 㺞䗴Ⲻ㴁ⲳ⴮ੂδP17εχ cεCP4 EPSPS 㴁ⲳֵὃ⢟㙆਍㥿⭎㟜䲚㥿ࡸθ㥿⭎㟜ᱥߒ䗴®㊱䲚㥿 ࡸⲺᴿ᭾ᡆ࠼δP17εχ

dεMON 87427 ⦿㊩ѣ CP4 EPSPS 㴁ⲳⲺ㺞䗴ޭᴿ㓺㓽䘿᤟ᙝθ᭯਎Ҽ ㊩ሯ㥿⭎㟜Ⲻᣍᙝਃᓊθֵ䈛ᙝ⣬ਥԛ֒Ѱж亯⦿㊩ᵸӚ࡬〃䙊⦿ ᖺֵ⭞δP17εχ

eεሯ MON 87427 䘑㺂Ҽཝ䠅Ⲻ࠼ᆆ⭕⢟ᆜ⢯ᙝ࠼᷆ȾSouthern ᵸӚ ࠼᷆㺞᱄φᶛ㠠 PV-ZMAP1043 䍞㋈Ⲻ T-DNA ᓅࡍ൞⦿㊩ะഖ㓺ঋ ж᮪ਾփ⛯ঋѠ᤭䍓ᨈޛδP16εχ

fε MON 87427 ѣ⋗ᴿỶ⎁ࡦф T-DNA ⴮䘔ᡌу⴮䘔Ⲻ PV-ZMAP1043 䍞㋈僞ᷬᓅࡍᡌެᆹཐ֏Ⲻ⡽⇫δP16εχ

gε࡟⭞伕૷㠪ᮅ⹊ガ਀䍺ⓆぁᓅᮦᦤᓉδFARRP_2010 ε δhppt://www.allergenonline.comεθሼᕋޛ㴁ⲳфᐨ⸛䗽ᮅ৕㴁ⲳ ≞ะ䞮ᓅࡍ䘑㺂⴪᧛∊ሯθỶ㍘Աᝅ 80 Ѡ≞ะ䞮⡽⇫䗴ࡦ 35%⴮ ղж㠪ᙝⲺ⡽⇫θԛ䇺ԭ CP4 EPSPS 㴁ⲳᱥੜޭᴿ▒൞Ⲻ㠪ᮅᙝ δP18Aεχ

hεᓅࡍ∊ሯ࠼᷆㔉᷒㺞᱄θCP4 EPSPS 㴁ⲳфᐨ⸛Ⲻ䗽ᮅ৕Ƚ哜䞽⓬ 㴁ⲳᡌ哜䉭㴁ⲳ⋗ᴿ≞ะ䞮ᓅࡍ⴮ղᙝδP18Aεχ

iε CP4 EPSPS 㴁ⲳ։ཌ⎾ौ㜳࣑⭞੡ᴿ㛹㴁ⲳ䞬Ⲻ⁗ᤕ㛹⏨δSGFε䘑㺂䇺ԭ⹊ガȾ⹊ガ䟽⭞Ⲻ㴁ⲳѰཝ㛖ᵼ㨂㺞䗴Ⲻθ⭞ SDS-PAGEȽ Western ᵸӚૂ䞬⍱ᙝ⎁ᇐ䘑㺂㔉᷒࠼᷆Ⱦ㔉᷒㺞᱄θཝ㛖ᵼ㨂㺞

227 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䗴Ⲻ CP4 EPSPS 㴁ⲳ൞⁗ᤕ㛹⏨ѣ㜳䗻䙕㻡⎾ौδP18A ૂ P18εχ

jε ሯཝ㛖ᵼ㨂ӝ⭕Ⲻ CP4 EPSPS 㴁ⲳ䘑㺂Ҽ✣どᇐᙝ䇺ԭȾᆫ㛨⑟ᓜ Ѱ 25°CȽ37°CȽ55°CȽ75°C ૂ 95°Cθᰬ䰪 30 ࠼䫕Ⱦ㔉᷒㺞᱄θ൞ 25°C ૂ 37°C сᆫ㛨੄θ㴁ⲳԃؓᤷ⍱ᙝȾ55°C сᆫ㛨੄㴁ⲳ⍱ᙝ 䲃քࡦ 25%θ㙂ᖉ㴁ⲳ㔅 75°C ૂ 95°C ᆫ㛨੄θ㴁ⲳ⍱ᙝ䲃քࡦỶ ⎁䲆ԛсȾр䘦ᮦᦤ㺞᱄儎✣༺⨼੄θ㴁ⲳ⍱ᙝуどᇐᡌ䲃քࡦỶ ⎁䲆ԛсδP18εχ

kεѰ䇺ԭ CP4 EPSPS 㴁ⲳⲺ∈ᙝθ࡟⭞ FASTA ∊ሯᐛޭθф TOX_2010 ∈㍖ᮦᦤᓉѣऻ੡Ⲻ 8448 ᶗ㴁ⲳᓅࡍ䘑㺂Ҽ∊ሯδP18εχ lε ⭕⢟ؗᚥᆜ࠼᷆㔉᷒㺞᱄θCP4 EPSPS 㴁ⲳᓅࡍфᮦᦤᓉѣⲺ∈㍖㴁ⲳуᆎ൞㔉ᶺ⴮ղᙝδP18εχ

mε ࡟⭞ཝ㛖ᵼ㨂ᶛⓆⲺ CP4 EPSPS 㴁ⲳ䘑㺂Ҽᙛᙝਙᵃ⹊ガȾཝ㛖 ᵼ㨂ᶛⓆ㴁ⲳф MON 87427 ⦿㊩㺞䗴㴁ⲳޭᴿ⨼ौᙝ䍞ૂࣕ㜳Ⲻ ㅿੂᙝχ

nε⋗ᴿਇ⧦ф㴁ⲳ⚂ஸ༺⨼⴮ީⲺѪᓀȽ⯻⨼ᡌ։䠃໔ࣖᖧଃδP18εχ

oε᤿➝ OECD ᤽঍θ䘑㺂Ҽ䈜㓼Ⲻ㓺ᡆᡆ࠼࠼᷆θԛ⺤ᇐ MON 87427 фᑮ㿺ሯ➝ૂ୼ѐौ৸➝ᵸӚ〃⴮∊θѱ㾷㩛ޱᡆ࠼Ƚᣍ㩛ޱᡆ࠼ ԛ਀⅗㓝ԙ䉘ӝ⢟≪ᒩᱥੜޭᴿਥ∊ᙝδP18εχ

pε㔉᷒㺞᱄θMON 87427 Ⲻᡆ࠼੡䠅≪ᒩфᑮ㿺ሯ➝⴮ᖉθҕኔӄ୼ ѐौ⦿㊩ᵸӚ〃Ⲻ⴮ᓊᡆ࠼㤹പҁ޻δP17AεȾ

ᦤᱥ 2002 ᒪ 740 ⌋ᗁȽ2005 ᒪ 4525 ⌋ԚȽ伕૷⌋ޮည઎דр䘦䇺ԭⲺ ޢ᭯⡾ᵢԛ਀⭩䈭ؤՐ CAC/GL 44-2003ȽCAC/GL 45-2003 ᤽ԚȽ2008 ᒪ ਮᡶ⭩䈭Ⲻ᢯߼⭞䙊χ

ᢶᵥည઎ՐδCTNSaludε2013 ᒪ 5 ᴾ 6 ᰛՐޞᦤр䘦ؗᚥθളᇬ⭕⢟ᆿד ࠰ྸсߩᇐφᔰ䇤ড⭕ф⽴Րؓ䳒䜞ㆴ㖨㺂᭵ᝅڐ䇤δՐ䇤䇦ᖋ㕌ਭφ2ε 㿷θ᢯߼р䘦⦿㊩䖢ौ։ MON-87427-7 ⭞֒Ӱ㊱⎾䍯伕૷ᡌࣖᐛ৕ᯏֵ⭞Ⱦ

228 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䢪ӄр䘦θ

ߩᇐφ

ᶗⅴ 1φ᢯߼ଛ՜∊Ӑߒѐᴿ䲆ޢਮδCOMPANIA AGRICOLA COLOMBIANA LTDA, T.I.N. 8300804353εԙ㺞ߒѐㆶौ㛗Գᴿ䲆ޢਮδCOMPANIA AGRICOLA S.A.Sθ ൦൶φ Bogota D.C. θ TIN. 830.080.640-7 ε⭩䈭Ⲻ⦿㊩ӝ૷ MON 87427 δMON-87427-7ε⭞֒Ӱ㊱伕૷⎾䍯Ⱦ

ᵢ᢯߼㠠ᵢߩ䇤⭕᭾ҁᰛ䎭θᴿ᭾ᵕ 5 ᒪθ᢯߼㤹പѰ᮪ѠളᇬȾᵢ᢯߼ཧ᭾ࢃ пѠᴾਥԛᨆ࠰㔣⭩䈭θ㔣⭩䈭ᴿ᭾ᵕ⴮ੂθ㔣⭩䈭ᨆӚ䍺ᯏ䴶㾷ㅜਾ䙐䗽 2002 䇘ȽᴵԙⲺ⭕⢟ཐṭᙝཝՐҁɅগຊ䎡ؤᒪㅢ 740 ਭ⌋Ԛ᢯߼᧛਍Ⲻᡌ䱺ࣖȽ 㓩⭕⢟ᢶᵥᆿޞ䇤ᇐҜɆ䱺Ԭ II Ⲻ㾷≸Ⱦ

ᶗⅴ 2φ䘑ਙ MON 87427δMON-87427-7ε⦿㊩⭞֒Ӱ㊱伕⭞⎾䍯ᡌ伕૷ࣖᐛ ৕ᯏⲺঋփ䴶㾷䚫ᆾଛ՜∊Ӑ䙐䗽 2002 ᒪ 740 ਭ⌋Ԛૂ 2011 ᒪ 4254 ਭߩ䇤䇘ᡌᴵԙ㿺ᇐȾؤㅢ 7 ᶗᡶ᢯߼Ⲻগຊ䎡㓩䇤ᇐҜㅢ 18 ⅴㅢ 2-aεᶗ਀ެ䱺ࣖȽ

ڛᶗⅴ 3φжᰜᴿᣛ䚉㺞᱄ MON 87427δMON-87427-7ε⦿㊩ᢶᵥⲺᓊ⭞Րሯ ᓭӝ⭕у㢥ᖧଃθѰ䱱↘Ƚ䚵ރȽࠅ䖱ᡌ᧝࡬у㢥ধᇩⲺਇ⭕θߒѐㆶौ㛗Գᴿ 䲆ޢਮδCOMPANIA AGRICOLA S.A.Sε䴶㾷䪾ሯ∅Ѡ䱬⇫䎭㥿伄䲟㇗⨼ᮽԬθ⭧ ളᇬ⭕⢟ᆿޞᢶᵥည઎ՐδCTNSaludεᆎẙȾ

ᶗⅴ 4φ䚫 ➝ 2002 ᒪ 1122 ⌋Ԛㅢ 34 ⅴⲺ b)ૂ c) ᶗૂ 2001 ᒪ 4254 ߩ䇤ㅢ 9 䇘ᡌᴵؤⅴ㿺ᇐθྸ᷒ᆎ൞ 1979 ᒪ 9 ਭ⌋Ԛૂ 1997 ᒪ 3075 ⌋Ԛᡌެ䱺ࣖȽ ԙ㿺ᇐᨅ䘦ⲺӁ᭻ਇ⭕θളᇬ㦥૷ૂ伕૷ⴇ⶙ቶδINVIMAεૂ൦ᯯড⭕䜞䰞㺂 ӄ MON 87427δMON-87427-7ε⦿㊩⭞֒Ӱ㊱伕⭞⎾䍯ᡌ伕૷ࣖᐛ৕ᯏީެֵ ⲺỶ僂Ƚⴇ⶙ૂ㇗⨼㚂㜳θਥԛ䟽⭞ᆿޞ᧠᯳θᒬ༺ԛ㖐ⅴȾ

ᶗⅴ 5φ䴶㾷Ө㠠ሼᵢߩ䇤޻ᇯ઀⸛ߒѐㆶौ㛗Գᴿ䲆ޢਮδCOMPANIA ᦤ⧦㺂ҿ䇤㺂᭵דAGRICOLA S.A.Sε⌋ᇐԙ㺞ᵢӰᡌެညᢎԙ㺞θᒬ䇟ެ⸛ᚿθ ⌋ޮㅢ 51 ⅴ㿺ᇐθྸሯᵢߩ䇤ᴿᔸ䇤θ䴶㾷൞ᵢ㺂᭵ߩ䇤⭕᭾䙐⸛ҁᰛ䎭 5 ᰛ ޻θр䇿ҁࢃᨆ࠰Ⱦ

⇫㩳φ᤿➝ҿ䇤㺂᭵⌋ޮㅢ 45 ⅴ㿺ᇐθྸ᷒ᵢߩ䇤у㜳Ө㠠䘷䗴θ䴶㾷Ҿԛޢ ᐹȾ

ᶗⅴ 6φᵢߩ䇤㠠ᇔ᯳ҁᰛ䎭⭕᭾Ⱦ

229 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 230 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 231 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 232 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 233 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 10Ƚଛ՜∊Ӑളᇬ⭕⢟ᢶᵥည઎Ր-ߒѐ⹊ガᡶ ᢯߼ᮽԬ δCTNbio / ICAθ侨⭞ε

234 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߩ䇤㕌ਭφ000424

2014 ᒪ 2 ᴾ 3 ᰛ

᢯߼ MON 87427(MON87427-7)⦿㊩⭞֒⴪᧛⎾䍯ᡌ֒Ѱ侨ᯏࣖᐛ৕ᯏ

ଛ՜∊Ӑߒѐ⹊ガᡶᙱ䍕䍙Ӱ

δTHE GENERAL MANAGER OF THE INSTITUTO COLOMBIANO AGROPECUARIO, ICAε

൞↚㺂ֵެ⌋ᗁᵹ࣑θ⢯ࡡᱥ 1992 ᒪ 2141 ਭ⌋ԚȽ1994 ᒪㅢ 1840 ਭ⌋ԚȽ 2005 ᒪㅢ 4525 ਭ⌋Ԛૂ 2008 ᒪㅢ 4765 ਭ⌋Ԛᡶ䎁ҾⲺ⌋ᗁᵹ࣑

䢪ӄφ

ളᇬ᭵ᓒᵰᶺ൞ 2002 ᒪ 740 ⌋ᗁ࡬ᇐ䗽ぁѣਇᐹ 2005 ᒪ 4525 ⌋Ԛθᒬ ᦾҾߒѐૂߒᶇਇኋ䜞䙐䗽ଛ՜∊Ӑߒѐቶߒѐ⹊ガᡶሯߒѐȽ⮒⢝Ƚ⑊ѐȽ୼ ѐૂߒӝ૷ࣖᐛѐ᷍ᵞ䚍Ֆ᭯㢥⭕⢟δGMOεⲺ䏀ູ䖢〱Ƚ䘆䗉ૂᬃ֒㺂ֵᇗ᢯ ᵹ࣑θ䘏ӑ GMO ᴿਥ㜳Րሯ⭕⢟ཐṭᙝⲺؓᣚૂਥᤷ㔣࡟⭞ӝ⭕у࡟ᖧଃχ 䚫➝ 2002 ᒪ 740 ⌋ᗁᡶ㿺ᇐⲺぁᓅθ2005 ᒪ 4525 ⌋Ԛ䇴ᇐҼ䚍Ֆ᭯㢥⭕ ⢟δGMOε⌋㿺Ṽᷬθᔰ㄁ҼᴿީߒѐȽ⮒⢝Ƚ⑊ѐȽ୼ѐૂߒӝ૷ࣖᐛѐ GMO Ⲻളᇬ⭕⢟ᆿޞᢶᵥည઎Րδ㾵⨣⢏䈣 CTNBioεθѱ㾷㚂䍙ᱥᔰ䇤 ICA ᙱ䍕䍙 Ӱㆴ㖨㺂᭵⌋Ṿθሯᓊ⭞䚍Ֆ᭯㢥⭕⢟Ⲻ⍱ࣞ䘑㺂ᦾᵹȾ ਮδCompania Agricola S.A.Sε֒Ѱ⭩䈭Էѐθޢଛ՜∊Ӑߒѐㆶौ㛗Գᴿ䲆䈭≸ ICA ൞⧦㺂⌋ᗁṼᷬ޻᢯߼ MON 87427δMON 87427-7ε⦿㊩൞ള޻⴪᧛ ⭞֒ࣞ⢟侨ᯏᡌ侨ᯏࣖᐛ৕ᯏȾ MON 87427 ⦿㊩㜳ཕӝ⭕ CP4 EPSPS 㴁ⲳθֵ⦿㊩ޭᴿ㥿⭎㟜䲚㥿ࡸᣍᙝθ ռф䈛ޢਮⲺ NK603 ⦿㊩ӝ૷уੂⲺᱥ㥿⭎㟜ᣍᙝޭᴿ㓺㓽⢯ᔸᙝθ㴁ⲳ൞㣧㊿ѣу㺞䗴ᡌ㺞䗴≪ᒩᶷքȾ

235 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 MON 87427 䖢ौ։ᱥ࡟⭞ߒᵼ㨂ԁሲ⌋䖢ौ LH198×HiII ⦿㊩૷㌱ᒲ㜐ӝ⭕ ᜾՚㓺㓽θ᜾՚㓺㓽фߒᵼ㨂ж䎭⭕䮵θ❬੄䖢〱ࡦ੡ᴿ㗝㣺䶈䴿㍖ૂ㥿⭎㟜Ⲻ ⭕䮵ฯޱะрθԛৱ䲚ߒᵼ㨂ૂᵠ㻡䖢ौⲺ᜾՚㓺㓽Ⱦ㆑䘿࠰Ⲻᆎ⍱㓼㜔ӝ⭕Ⲻ ὃṠ㠠Ӛθ⴪㠩䘿᤟ࡦᨈޛะഖ㓥ਾⲺὃṠȾ 䖢ौ䍞㋈Ѱ PV-ZMAP1043θཝቅ 8.9 kbȾ䈛䍞㋈੡ᴿߒᵼ㨂 CP4 㨂Ṡ cp4 epeps ะഖ㺞䗴ⴈθcp4 epsps ะഖ਍ᶛ㠠ӄ㣧ὦ㨒㣧ਬ⯻∈ CaMV 35S ੥ࣞ ᆆૂ⦿㊩✣◶㴁ⲳ hsp 70 ะഖ޻੡ᆆᓅࡍⲺ䈹᧝θ䘎਍ᶛ㠠ӄᤕ঍㣛䖢〱㛳侦θ䈛ᓅࡍ㜳ཕ᤽ሲ CP4 EPEPS 㴁ⲳੇਬ㔵։䖢〱Ⱦ↚ཌθཐ㚐㞰㤭ؤCTP2 Ⲻ ؗਭᶛ㠠ṯⲂߒᵼ㨂㜣㜸⻧ਾ䞬ะഖ nos Ⲻ 3’ㄥ䶔㘱䈇ᓅࡍȾ Southern ᵸӚ࠼᷆䇷ᇔҼᨈޛ⡽⇫൞ 5 Ѡьԙѣどᇐᆎ൞Ⱦ࠼ᆆ⭕⢟ᆜૂ ᙝ⣬࠼⿱࠼᷆㺞᱄θᨈޛᓅࡍѰঋжփ⛯ᨈޛθ੄ԙ䚍Ֆᙝ⣬Ⲻ࠼⿱ㅜਾᆕᗭቊ ᇐᗁȾ ᙝ⣬᭾᷒䈋僂ᱴ⽰ MON 87427 ⦿㊩䖢ौ։ޭᴿ㥿⭎㟜ᣍᙝȾռ MON 87427 фᑮ㿺ሯ➝⴮∊θ䲚Ҽ㥿⭎㟜䲚㥿ࡸᣍᙝԛཌθуᆎ൞Ա֋ᖘᘷᆜᐤᔸȾ ⧥ᓭૂڛ⭞়ਨθሯֵޞCP4 EPSPS 㴁ⲳ൞⦿㊩ૂެᆹ֒⢟рޭᴿ䮵ᵕⲺᆿ ູ⋗ᴿӝ⭕䗽у㢥ᖧଃȾ ެᆹ䖢ौ։ᡶ㺞䗴Ⲻ CP4 EPSPS 㴁ⲳᡶ䘑㺂Ⲻ侨ஸ䈋僂㺞᱄θ䈛㴁ⲳሯத喵㊱ࣞ⢟Ƚ㴁呗Ƚ劲Ƚ⥠ૂྫྷ⢑уޭᴿ∈ᙝ᭾ᓊȾ

⭞㺞䗴 CP4 EPSPS 㴁ⲳⲺ⦿㊩侨ஸ㚿呗θ⋗ᴿਇ⧦Ա֋㩛ޱу㢥㺞⧦θҕ⋗ ᴿਇ⧦ਇ㛨ૂ呗㚿⢯ᙝㅿᯯ䶘ф⭞ᑮ㿺⦿㊩侨ஸⲺ㚿呗ᆎ൞ᱴ㪍ᙝᐤᔸȾ ⭕⢟ؗᚥᆜ࠼᷆㺞᱄ CP4 EPSPS 㴁ⲳфᐨ⸛㠪ᮅ৕⋗ᴿੂⓆᙝȾ 䢪ӄр䘦θਥԛᗍ࠰㔉䇰φMON 87427 㺞䗴Ⲻ㴁ⲳуޭᴿ㠪ᮅ伄䲟Ⱦ 㘹㲇ࡦр䘦৕ഖθ2013 ᒪ 8 ᴾ 9 ᰛ⭧⧥ູфਥᤷ㔣ਇኋ䜞Ƚড⭕ф⽴Րؓ 䳒䜞Ƚߒѐૂߒᶇਇኋ䜞ȽColciencias ૂ ICA ࠰ᑣⲺ⭕⢟ᆿޞᢶᵥည઎Ր δCTNBioεㅢ 25 ⅗Ր䇤ᨆӚҼ“MON 87427δMON 87427-7ε⦿㊩൞ള޻⴪᧛ ⭞֒侨ᯏᡌ֒Ѱ侨ᯏࣖᐛ৕ᯏⲺ伄䲟䇺ԭ”㔉᷒θфՐ㘻䗴ᡆж㠪ᝅ㿷θᔰ䇤 ICA ᢯߼ MON 87427δMON 87427-7ε⦿㊩⴪᧛⭞֒侨ᯏ⎾䍯ᡌ֒Ѱ侨ᯏࣖᐛ৕ᯏȾ

236 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䢪ӄр䘦φ

ߩᇐφ

ᶗⅴ 1φ ᢯߼ଛ՜∊Ӑߒѐㆶौ㛗Գᴿ䲆ޢਮδCompania Agricola Colombiana S.A.Sθ TIN. 830.080.640-7θ൦൶φBogotaθ⌋Ӱԙ㺞 ANDRES GULLEN GOMEZε Ⲻ⦿㊩䖢ौ։ MON 87427δMON 87427-7ε൞ള޻⴪᧛⭞֒侨ᯏᡌ֒Ѱ侨ᯏࣖ ᐛ৕ᯏχ

ᙝ৕ഖθԱ֋ᰬُྸ᷒ ICA 䇚Ѱᴿᗻ㾷θਥ㜳ᵠ㔅ޞᶗⅴ 2φ࠰ӄਾ⨼Ⲻ⭕⢟ᆿ ᵹ࡟ਢഔ↚ᇗ᢯ߩ䇤χڵӁݾੂᝅᡌ⋗ᴿԱ֋䎊

⎾ᶗⅴ 3φֵ⭞⦿㊩䖢ौ։ MON 87427δMON 87427-7ε൞ള޻⴪᧛⭞֒侨ᯏ ਮ䴶㾷䚫ᆾ 2005 ᒪ 4525 ⌋ԚᶗⅴૂެԌ⴮ީ㿺ᇐχޢ䍯ᡌ֒Ѱ侨ᯏࣖᐛ৕ᯏⲺ

⎾ᶗⅴ 4φֵ⭞⦿㊩䖢ौ։ MON 87427δMON 87427-7ε൞ള޻⴪᧛⭞֒侨ᯏ ਮ䴶ታԛсѿࣗφޢ䍯ᡌ֒Ѱ侨ᯏࣖᐛ৕ᯏⲺ ࡟⭞ᵢߩ䇤᢯߼Ⲻ MON 87427 ⴪᧛⭞֒侨ᯏᡌ侨ᯏࣖᐛ৕ᯏχ .1 ਮᗻ䴶ੂᝅ ICA Ѱᢝ㺂ެ㚂䍙ᡶᗻ䴶Ⲻ僂䇷Ƚⴇ⶙Ƚ᧝࡬ૂ਌ṭᐛ֒χޢ .2 3. ྸ᷒ਇ⧦Ա֋ᐨ㔅ਇ⭕Ⲻᡌ঩ሼਇ⭕Ⲻᖧଃ⭕⢟ᆿޞⲺ伄䲟ᡌᦕᇩθᓊᖉ ㄁঩ᣛ઀ ICAχ 4. ྸ䙽㍝ᙛ᛻߫θ㄁঩䟽਌ᴿ᭾Ⲻ㕉䀙᧠᯳χ 5. 䚫ᆾߒӝ૷ᯯ䶘Ⲻެᆹ㿺ᇐȾ

ᶗⅴ 5φ䘓ਃᵢߩ䇤θICA ሼ᤿➝ 1994 ᒪ 1840 ⌋ԚȽ2005 ᒪ 4525 ⌋Ԛᡌެ ⨽ᯯ䶘ᖈኔⲺ᛻߫сθ㺂ֵ㇗ޞ䇘ᡌᴵԙ㿺ᇐθ൞уᦕᇩ ICA ެᆹ⭕⢟ᆿؤ⴮ᓊ ༺㖐㚂䍙χ

ᶗⅴ 6φ᤿➝ 2005 ᒪ 4525 ⌋Ԛ㿺ᇐθᵢߩ䇤ሼ൞ԛс ICA 㖇享рҾԛޢᐹ www.ica.gov.co.;

ᶗⅴ 7φṯᦤҿ䇲ᙝ㺂᭵⍷ぁᆾࡏδ2011 ᒪ 1437 ⌋ᗁεㅢ 67-69 ᶗⅴᨆӚᵢ㺂 ᭵⌋Ṿχ

ᶗⅴ 8φྸሯᵢߩ䇤ᆎ൞ᔸ䇤θᨆ䈭⭩䈭᫚䬶ᵢߩ䇤θṯᦤҿ䇲ᙝ㺂᭵⍷ぁᆾࡏ ઀ਇᐹ੄ 10 Ѡᐛ֒ᰛ޻ᨆ࠰Ⱦޢδ2011 ᒪ 1437 ⌋ᗁεㅢ 76 ᶗ㿺ᇐθᗻ亱ӄ

ᶗⅴ 9φᵢߩ䇤㠠ㆴ㖨ҁᰛ䎭⭕᭾Ⱦ

237 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 238 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 239 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 240 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 241 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 11Ƚ㨨ᗁᇴὃ⢟ӝѐቶ᢯߼ᮽԬ δBPIθ伕⭞/侨⭞ε

242 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 㨨ᗁᇴާૂള

ߒѐ䜞 ὃ⢟ӝѐቶ 692 San Andres St., Malate, Manila

䇮ਥޞ⴪᧛⭞ӄ伕૷ૂ侨ᯏԛ਀ࣖᐛֵ⭞Ⲻ⭕⢟ᆿ ㅢ 14-079 ਭ

ሯᆕኧ䜳ޢਮ㨨ᗁᇴ࠼ޢਮԙ㺞༺δ൦൶φ23m Insular Life Building, Insular Drive, Alabang, Muntinlupa CityεᔶਇⲺ MON 87427 ⦿㊩ૂ䈛ӝ૷ᵸӚᡶᗍᡶᴿ ੄ԙδ䲚ҼфެԌ⭕⢟ᢶᵥᙝ⣬Ⲻགྷਾ੄ԙε䘑㺂Ҽ⭕⢟ᆿޞᙝ䇺ԭθ㔉᷒ԚӰ θᒬਥ֒Ѱᑮ㿺ੂ㊱ӝ૷Ⲻᴵԙ૷⭞֒伕૷Ƚޞᝅθ䇚Ѱެфᑮ㿺⦿㊩ੂṭᆿ┗ 䇮ਥ䇷⴪᧛ޞ侨ᯏૂࣖᐛ৕ᯏȾ↚ཌθᵢ䇮ਥ䇷ᤷᴿ㘻ᖾླ൦䚫ᗠҼ⺤ؓ⭕⢟ᆿ ⭞ӄ伕૷ૂ侨ᯏԛ਀ࣖᐛֵ⭞Ⲻᡶᴿ㾷≸Ⱦ ⴇ㇗ᶆᯏӻݷ䇮䘑ਙ⴪᧛⭞ӄ伕⭞Ƚ侨⭞ԛ਀ࣖᐛ⭞䙊θуᗍ⭞ӄ⭦䰪䈋僂 ᓭԛ਀⧥ູθᒬڛ⢟ᡌ㑷㺃ᶆᯏȾ䇮ਥ䇷ᤷᴿ㘻ᓊᖉ䟽਌䘸ᖉ᧠᯳ؓᣚӰ㊱ૂࣞ ᙝ䶔᭻ᝅ㑷⇌ӁԬ䠃ਇȾᓊᖉݷ䇮ὃ⢟Ỷ⯡ᇎ઎਀ެ↙ᕅᦾᵹԙ㺞ӄᐛ❭ڬ↘䱨 ᰬ䰪ᇐᵕࢃᖶⴇ㇗ᶆᯏᆎ᭴ᵰᶺθᒬḛⵁфⴇ㇗ᶆᯏ䘑ਙ⴮ީⲺ䇦ᖋȾ䇮ਥ䇷֒ ᤷᴿ㘻ᓊᖉᇐᵕ൞ള޻ᣛ㓮р઀⸛䈛ӝ૷Ⲻ䘑ਙ൞ 2003 ᒪߒѐ䜞༽ᘎᖋ䙐઀ 2003 ᒪㅢ 8 ᶗᡶ᢯߼Ⲻᶗⅴ䇮ਥᵹ࣑㤹പ޻Ⱦ↚㊱ޢ઀ᓊᖉ൞ਇ㺞੄δ10εཟ ޻䙈Ӛὃ⢟ӝѐቶȾ ᦤ 2002 ᒪߒѐ䜞㺂᭵ળԚㅢ 8 ᶗ㿺ᇐθ2007 ᒪߒѐ䜞㺂᭵ળԚㅢ 22 ᶗד 䇘Ⲻ⴮ީ޻ᇯθ䘓ਃр䘦ᶗԬ㘻ሼ㻡᫚䬶ᵢ䇮ਥ䇷Ⱦؤ ᦤ 2002 ᒪߒѐ䜞㺂᭵ળԚㅢ 8 ᶗ㿺ᇐθ2007 ᒪߒѐ䜞㺂᭵ળԚㅢ 22 ᶗד 䇘ѣᨆ࠰Ⲻ⴮ީᶗⅴૂᶗԬθᵢ䇮ਥ䇷ᴿ᭾ᵕѰ 5 ᒪθሼӄ 2019 ᒪ 11 ᴾ 27ؤ ᰛཧ᭾Ⱦ ㆴਇӄ 2014 ᒪ 11 ᴾ 28 ᰛθὃ⢟ӝѐቶδ൦൶φSan Andres St., Malate, ManilaεȾ

PAZ J. BENAVIDEZ II ⌋㿺ࣟ⨼『Ҝ䮵޲ OIC ѱ㇗θὃ⢟ӝѐቶ

243 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 244 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 12Ƚ䏀঍ߒѐૂߒᶇਇኋ䜞᢯߼ᮽԬ δMARDθ伕⭞ૂ侨⭞ε

245 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߒѐфߒᶇਇኋ䜞䏀঍⽴Րѱѿާૂള δMARDε ⤢㄁Ƚ㠠⭧Ƚᒮ⿅ ———————————— —————————— No.: 3659/QD-BNN-KHCN ⋩޻ᐸθ2015 ᒪ 9 ᴾ 9 ᰛ ߩ 䇤

䖢ะഖ֒⢟Ⲻ伕⭞ૂ侨⭞᢯߼

ߒѐфߒᶇਇኋ䜞δMARDε

⨽ᦤ 2013 ᒪ 11 ᴾ 26 ᰛਇᐹⲺީӄߒѐфߒᶇਇኋ䜞δMARDε᭵ᓒ㇗ד 㚂㜳ȽԱࣗȽᵹ࣑ૂ㓺㓽㔉ᶺⲺㅢ 199/2013/ND-CP ਭߩ䇤χ ⢟ᦤ 2010 ᒪ 6 ᴾ 21 ᰛਇᐹⲺީӄሯ䖢ะഖ⭕⢟Ƚ䚍Ֆ⢟䍞ૂ䖢ะഖ⭕ד ӝ૷Ⲻ⭕⢟ᆿޞ㇗⨼Ⲻㅢ 69/2010/ND-CP ਭߩ䇤ᮽԬȽԛ਀ 2011 ᒪ 11 ᴾ 30 䇘ᶗⅴѣ⴮ީ㿺ᇐχؤᰛਇᐹⲺㅢ 108/2011/ND-CP ਭ 䘑㺂⌋㿺ⴇ㇗Ⲻㅢ⌊ޞᦤ 2012 ᒪ 4 ᴾ 25 ᰛਇᐹⲺީӄሯᇔ᯳伕૷ᆿד 38/2012/ND-CP ਭߩ䇤χ ᦤ 2014 ᒪ 1 ᴾ 24 ᰛਇᐹⲺީӄ䖢ะഖὃ⢟⴪᧛⭞ӄ伕⭞ૂ侨⭞Ⲻ᢯߼ד ⍷ぁⲺㅢ 02/2014/TT-BNNPTNT ਭޢ઀χ 䇺ԭՐ䇤ޞည઎Ր㓺㓽Ⲻᆿޞᦤ 2015 ᒪ 1 ᴾ 18 ᰛ⭧䖢ะഖ⭕⢟伕⭞ᆿד ԛ਀Ր䇤䇦ᖋχ ᓊ〇ᆜᢶᵥૂ⧥ູ࠼䜞ѱԱ㾷≸θ

⢯↚ߩ䇤φ ㅢжθᡶ䘦伕⭞ૂ侨⭞Ⲻ᢯߼䘸⭞㓼㢸ྸсφ 1. ᐨ㧭᢯߼伕⭞ૂ侨⭞Ⲻ䖢ौ։Ѱφ

੃φ⦿㊩؍ - - ᣿зᆜ੃φZea mays L. - ୼૷੃φߒ䗴ᵸӚ⦿㊩㌱㔕δRHSε - 䖢ौ։੃〦φMON 87427 - ୥ж䇼ࡡਭφMON-87247-7 - фᕋޛะഖ⴮ީⲺᙝ⣬φ㓺㓽⢯ᔸᙝᣍ㥿⭎㟜䲚㥿ࡸ 2. ⭩䈭ᵰᶺ

246 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 - 䘠গ䏀঍ᴿ䲆ޢਮ - 㜗ᘍ᱄ᐸ 3-6 ॰θ䱤≅᱄ᔶ䐥 72-74 ਭθѣᗹཝড় 1303 ᇚ 3. ⭩䈭ᵰᶺ㚂䍙 ᓭ⋗ᴿ伄䲟χڛ⢟ᵢ᢯߼ะӄᡶᨆӚᮦᦤ⺤䇚ᡶ䘦ӝ૷ሯӰ㊱ૂࣞ - ਮᴿ䍙Ա⺤ؓ MON 87427 ⴮ީӝ૷൞ᵢ᢯߼㿺ᇐޢ䘠গ䏀঍ᴿ䲆 - 㤹പ޻Ⲻਾ⨼ֵ⭞χ ਮᴿ䍙Աᤷ㔣ሯᡶ䘦ӝ૷䘑㺂ⴇ⎁θԛ⺤ؓ䈛ӝ૷ޢ䘠গ䏀঍ᴿ䲆 - ㅜਾ 2014 ᒪ 1 ᴾ 24 ᰛਇᐹⲺㅢ 02/2014/TT-BNNPTNT ޢ઀ㅢ 17 ᶗȽԛ਀䏀঍⽴Րѱѿާૂളᡶᴿީӄ䖢ะഖ⭕⢟Ⲻ⴮ީ⌋ᗁ ⌋㿺Ⱦ ㅢӂθᵢߩ䇤ӄㆴᆍҁᰛ䎭⭕᭾Ⱦ ㅢпθᵢߩ䇤⭧ MARD ㇗⨼ࣔޢᇚѱԱȽ〇ᆜᢶᵥૂ⧥ູ࠼䜞ѱԱȽԛ਀ ࡍӄㅢжᶗѣⲺ⴮ީ㓺㓽ૂѠӰާੂᇂᡆȾ

᭬ԬӰφࢥ 䜞䮵 - ྸㅢпᶗᡶ䘦χ δㆴᆍȽⴌㄖε - MARD 䜞䮵δ઀⸛εχ - MoNRE Le Quoc Doanh - ᖈẙφVT. KHCN (TTQ.10b)

247 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ߒѐфߒᶇਇኋ䜞䏀঍⽴Րѱѿާૂള δMARDε ⤢㄁Ƚ㠠⭧Ƚᒮ⿅ ———————————— —————————— No.: 3659/QD-BNN-KHCN ⋩޻ᐸθ2015 ᒪ 9 ᴾ 9 ᰛ 䇷 Ҝ

䖢ะഖ֒⢟Ⲻ伕⭞ૂ侨⭞᢯߼ δ䳅 2015 ᒪ 9 ᴾ 9 ᰛ MARD No.: 3659/QD-BNN-KHCN ਭޢ઀жᒬਇᐹε

1. ᐨ㧭᢯߼伕⭞ૂ侨⭞Ⲻ䖢ौ։Ѱφ ੃φ⦿㊩؍ - - ᣿зᆜ੃φZea mays L. - ୼૷੃φߒ䗴ᵸӚ⦿㊩㌱㔕δRHSε - 䖢ौ։੃〦φMON 87427 - ୥ж䇼ࡡਭφMON-87247-7 - фᕋޛะഖ⴮ީⲺᙝ⣬φ㓺㓽⢯ᔸᙝᣍ㥿⭎㟜䲚㥿ࡸ 2. ⭩䈭ᵰᶺ - 䘠গ䏀঍ᴿ䲆ޢਮ - 㜗ᘍ᱄ᐸ 3-6 ॰θ䱤≅᱄ᔶ䐥 72-74 ਭθѣᗹཝড় 1303 ᇚ 3. ⭩䈭ᵰᶺ㚂䍙 ᓭ⋗ᴿ伄䲟χڛ⢟ᵢ᢯߼ะӄᡶᨆӚᮦᦤ⺤䇚ᡶ䘦ӝ૷ሯӰ㊱ૂࣞ - ਮᴿ䍙Ա⺤ؓ MON 87427 ⴮ީӝ૷൞ᵢ᢯߼㿺ᇐ㤹ޢ䘠গ䏀঍ᴿ䲆 - പ޻Ⲻਾ⨼ֵ⭞χ ਮᴿ䍙Աᤷ㔣ሯᡶ䘦ӝ૷䘑㺂ⴇ⎁θԛ⺤ؓ䈛ӝ૷ㅜޢ䘠গ䏀঍ᴿ䲆 - ਾ 2014 ᒪ 1 ᴾ 24 ᰛਇᐹⲺㅢ 02/2014/TT-BNNPTNT ޢ઀ㅢ 17 ᶗȽ ԛ਀䏀঍⽴Րѱѿާૂളᡶᴿީӄ䖢ะഖ⭕⢟Ⲻ⴮ީ⌋ᗁ⌋㿺Ⱦ

ࢥ䜞䮵 ᢩ߶ਧ˖014-15/GXNGMO-BNN δㆴᆍȽⴌㄖε

Le Quoc Doanh

248 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 249 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 250 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 251 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 13Ƚ⅝ⴕည઎Ր᢯߼ᮽԬ δECθ伕⭞/侨⭞ε

252 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⅝ⴕည઎Րᢝ㺂ߩ䇤δEUε2015/2281

2015 ᒪ 12 ᴾ 4 ᰛ ᦤ⅝⍨䇤Րૂ⅝ⴕ⨼ӁՐⲺㅢ 1829/2003 ਭ⌋㿺δECε᢯߼䚍Ֆ᭯㢥⦿㊩ד MON87427δMON-87427-7ε਀ެӝ૷ૂࣖᐛӝ૷䘑ޛᐸ൰Ⲻߩᇐ

δ䙐⸛৸㿷ᮽԬ C(2015) 8587ε

δ䙐⸛ӻ⭞ᇎᯯ䈣䀶φ⌋䈣ૂ㦭ަ䈣ε

δText with EEA relevanceε

⅝ⴕည઎Ր

ṯᦤ⅝ⴕ㚂䍙Ⲻঅ䇤θԛ਀

ṯᦤ⅝⍨䇤Րૂ⅝ⴕ⨼ӁՐӄ 2003 ᒪ 9 ᴾ 22 ᰛ亷ᐹⲺީӄ䚍Ֆ᭯㢥伕૷ ૂ侨ᯏ(1)Ⲻ⌋㿺δECεㅢ 1829/2003 ਭθ⢯ࡡᱥެѣㅢ 7 ᶗㅢ 3 ⅴૂㅢ 19 ᶗ ㅢ 3 ⅴⲺ㿺ᇐθ

䢪ӄφ

דਮδMonsanto Europe S.A.εӄ 2012 ᒪ 6 ᴾ 11 ᰛޢᆕኧ䜳⅝⍨㛗Գᴿ䲆 (1) ᦤ⌋㿺δECεㅢ 1829/2003 ਭѣㅢ 5 ᶗૂㅢ 17 ᶗ䙐䗽㦭ަ᭵ᓒѱ㇗䜞䰞䙈ӚҼ MON87427 ⦿㊩֒Ѱ伕૷Ƚ伕૷ᡆ࠼ԛ਀侨ᯏрᐸⲺ⭩䈭δԛсㆶ 〦“⭩䈭”εȾ

䈛⭩䈭䘎⎿਀ MON87427 ⦿㊩Ƚӝ૷ૂެࣖᐛӝ૷䲚Ҽ֒Ѱ伕૷ૂ侨ᯏԛ (2) ཌެԌ⭞䙊Ⲻрᐸ⭩䈭θռ䈛⭩䈭уऻᤢ〃ὃ⭞䙊Ⱦ

(3) ᤿➝ㅢ 1829/2003 ਭ⌋㿺δECεѣㅢ 5 ᶗㅢ 5 ⅴૂㅢ 17 ᶗㅢ 5 ⅴⲺ㿺ᇐθ 䈛⭩䈭ऻᤢҼ⅝⍨䇤Րૂ⅝ⴕ⨼ӁՐ亷ᐹⲺ 2001/18/EC ᤽ԚѣⲺ䱺Ԭ III ᦤ 2001/18/EC ᤽ד䇺ԭᮦᦤૂ䍺ᯏθԛ਀ऻᤢҼޞ䱺Ԭ IV ᡶ㾷≸Ⲻᆿૂ ᦤ 2001/18/EC ᤽ד䇺ԭ㔉䇰Ⱦ䈛⭩䈭䘎ऻᤢҼޞԚѣ䱺Ԭ II ᡶ֒࠰Ⲻᆿ Ԛѣ䱺Ԭ VII ᡶ㾷≸Ⲻሯ⧥ູᖧଃⲺⴇ᧝䇗ࡈȾ

ᦤ⌋㿺δECεㅢ 1829/2003 ਭѣㅢ 6 ᶗૂㅢדቶδEFSAεޞⴕ伕૷ᆿ⅝ (4) Ⲻ㔉䇰ȾEFSA 㔉䇰䇚Ѱθྸ䈛ޞ࠰䇚Ѱެᆿڐᶗӄ 2015 ᒪ 6 ᴾ 19 ᰛ 18 ⭩䈭ѣᡶ䘦θMON87427 ⦿㊩фެ⴮ᓊⲺ䶔䚍Ֆ᭯㢥ሯ➝⦿㊩൞⭩䈭ѣᡶ ޞᓭᡌ㘻ᱥ⧥ູᆿڛ⢟ⲺθуՐሯӰ㊱ૂࣞޞᨅ䘦Ⲻ⭞䙊(3)ᯯ䶘ੂṭᱥᆿ ᑜᶛу࡟ᖧଃȾ

253 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᦤ⌋㿺ㅢ 6 ᶗㅢ 4 ⅴד࠰㔉䇰ᰬθEFSA 㘹㲇Ҽ਺ᡆ઎ള᭵ᓒⴇ㇗ᵰᶺڐ൞ (5) ૂㅢ 18 ᶗㅢ 4 ⅴᨆ࠰Ⲻᡶᴿ䰤从ૂ亴㲇Ⱦ

࠰㔉䇰ᰬθEFSA 䘎ᗍ࠰㔉䇰䇚Ѱ⭩䈭Ҝѣᨆ࠰Ⲻ⧥ູⴇ㇗䇗ࡈδж㡢ڐ൞ (6) ᙝⴇ㇗䇗ࡈεфެ⴮ᓊ⭞䙊ᱥੱਾⲺȾ

(7) 㔲рᡶ䘦θMON87427 ⦿㊩ȽMON87427 Ⲻӝ૷ԛ਀ެࣖᐛӝ૷ᓊ䈛㧭ᗍ ᢯߼рᐸȾ

(8) ṯᦤ⅝ⴕည઎ՐⲺ⌋㿺δECεδㅢ 65/2004 ਭ(4)εθᡶᴿ䚍Ֆ᭯㢥⭕⢟ δԛсㆶ〦“GMO”ε䜳ᓊ䈛䎁Ҿ୥ж䇼ࡡਭȾ

(9) ṯᦤ EFSA Ⲻᝅ㿷θ䲚Ҽ⌋㿺δECεㅢ 1829/2003 ਭѣㅢ 13 ᶗㅢ 1 ⅴૂ ㅢ 25 ᶗㅢ 2 ⅴ㿺ᇐⲺḽ䇼㾷≸ཌθу䴶㾷ሯ MON87427 ⦿㊩Ƚ਀ެӝ૷ ࣖᐛӝ૷Ⲻḽ䇼ᨆ࠰仓ཌ㾷≸Ⱦ❬㙂θѰҼؓ䇷䈛ӝ૷ਠ㜳⭞ӄᵢߩᇐૂ ᢯߼Ⲻ⭞䙊θྸ᷒ MON87427 ӝ૷ૂ੡ᴿ MON87427 Ⲻӝ૷⭞ӄ伕૷ԛ ཌⲺ⭞䙊θᓊ䈛␻ᾐ൦⌞᱄䈛ӝ૷у㜳⭞ӄ〃ὃⴤⲺȾ

(10) ⅝ⴕ䇤Րૂ⅝ⴕ⨼ӁՐ亷ᐹⲺ⌋㿺δECεㅢ 1830/2003 ਭѣⲺㅢ 4 ᶗㅢ 6 ࠰Ҽ㿺ᇐθᴿީⓥⓆᙝḽ䇼Ⲻڐⅴሯ GMO ӝ૷ૂ੡ᴿ GMO Ⲻӝ૷Ⲻḽ䇼㾷≸㿷ㅢ 4 ᶗㅢ 1-5 ⅴθᴿީ伕⭞ૂ侨⭞ḽ䇼Ⲻ㾷≸㿷ㅢ 5 ᶗȾ

(11) ᢯߼ᤷᴿӰᓊ䈛∅ᒪᨆӚ⧥ູ᭾ᓊⴇ᧝䇗ࡈⲺᢝ㺂᛻߫ૂ㔉᷒Ⲻᣛ઀Ⱦ䘏 ӑ㔉᷒≽ᙱⲺᖘᕅᓊㅜਾ⅝ⴕည઎Րㅢ 2009/770/EC(2) ਭߩ䇤Ⲻ㾷≸Ⱦ ᦤ⌋㿺δECεㅢ 1829/2003 ਭㅢ 6 ᶗㅢ 5 ⅴૂㅢ 18 ᶗㅢ 5 ⅴⲺ㿺ᇐθד EFSA Ⲻᝅ㿷ᒬᵠ⢯ࡡ㾷≸ӝ૷рᐸδૂ/ᡌӝ૷Ⲻֵ⭞ૂ༺⨼θऻᤢ֒Ѱ 伕⭞ૂ侨⭞Ⲻᐸ൰ⴇ㇗εⲺ⢯ᇐᶗԬᡌ䲆࡬θҕᵠሯ⢯⇀⭕ᘷ㌱㔕/⧥ູૂ ࠰䲆ᇐȾڐᡌ൦⨼॰ตⲺؓᣚ/

ṯᦤ⌋㿺δECεㅢ 1829/2003 ਭⲺ㿺ᇐθᡶᴿީӄӝ૷᢯߼Ⲻؗᚥᓊ䈛൞ (12) 䚍Ֆ᭯㢥伕૷ૂ侨ᯏⲺည઎Րⲱ䇦ᮽԬѣᣡ䵨Ⱦ

(13) ṯᦤ⅝⍨䇤Րૂ⅝ⴕ⨼ӁՐ亷ᐹⲺㅢ 1946/2003 ਭ⌋㿺δECε(3)ㅢ 9 ᶗㅢ ؗᚥӚ⍷ѣᗹޞⅴૂㅢ 15 ᶗㅢ 2c ⅴⲺ㿺ᇐθᵢߩᇐՐ䙐䗽⭕⢟ᆿ 1 δBiosafety Clearing-Houseε䙐⸛⭕⢟ཐṭᙝޢ㓜Ⲻগຊ䎡䛙⭕⢟ᆿޞ䇤 ᇐҜ㕊㓜ളȾ

(14) 䇚Ѱᴿᗻ㾷࡬ᇐжѠᇔ᯳Ⲻ㺂ࣞᯯṾθᒬъѱᑣҕᨆӚҼᇔ᯳ᯯṾⲺ㥿ふ ᓭᑮညՐᵠ൞ѱᑣ㿺ڛ⢟ᓊ䬴ૂࣞב⢟㾷≸р䇿ည઎Ր䘑ж↛㘹㲇Ⱦռ伕 ᇐⲺᰬ䰪޻ᨆ࠰ᝅ㿷Ⱦഖ↚θр䇿ည઎Րᵠሯ↚ᖘᡆᝅ㿷Ⱦ

254 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᵢߩᇐᐨ㻡䟽⭞⭕᭾Ⱦ

ㅢжᶗ

䚍Ֆ᭯㢥⭕⢟਀ެ୥ж䇼ࡡਭ

ṯᦤ⌋㿺δECεㅢ 65/2004 Ⲻ㿺ᇐθᵢߩᇐ䱺Ԭѣ b ⛯ᡶ᤽ MON87427 䚍Ֆ᭯ 㢥⦿㊩δZea mays L.εⲺ୥ж䇼ࡡਭѰ MON-87427-7Ⱦ

ㅢӂᶗ

᢯߼

ṯᦤ⌋㿺δECεㅢ 1829/2003 ਭㅢ 4 ᶗㅢ 2 ⅴૂㅢ 16 ᶗㅢ 2 ⅴ㿺ᇐⲺ⭞䙊θ с䘦ӝ૷㧭ᗍҼ᢯߼φ

(a) ੡ᴿ MON87427-7 ⦿㊩Ƚӝ૷਀ެࣖᐛⲺ伕૷ૂ伕૷ᡆ࠼χ

(b) ੡ᴿ MON87427-7 ⦿㊩Ƚӝ૷਀ެࣖᐛⲺ侨ᯏ

ㅢпᶗ

ḽ䇼

1. ṯᦤ⌋㿺δECεㅢ 1829/2003 ਭㅢ 13 ᶗㅢ 1 ⅴૂㅢ 25 ᶗㅢ 2 ⅴԛ਀ ⌋㿺δECεㅢ 1830/2003 ਭㅢ 4 ᶗㅢ 6 ⅴᡶ㿺ᇐⲺḽ䇼㾷≸ⲺⴤⲺθ“⭕⢟։੃ 〦”ᓊ䈛Ѱ“⦿㊩”Ⱦ

2. ྸ᷒ MON87427-9 ⦿㊩਀ެᶺᡆӝ૷⭞ӄр䘦ㅢӂᶗδaεᡶ䘦⭞䙊ҁ ཌθᓊ䈛൞ḽㆴૂ⴮ީᮽԬѣḽ᱄“уਥ⭞ӄ〃ὃ”ⲺᆍṭȾ

ㅢ഑ᶗ

ሯ⧥ູᖧଃⲺⴇ᧝

1. ᢯߼ᤷᴿӰᓊ䈛ṯᦤ䱺Ԭδhε⛯ᡶࡍ᱄Ⲻ㾷≸࡬ᇐᒬᢝ㺂ሯ⧥ູᖧଃ Ⲻⴇ᧝䇗ࡈȾ

2. ᢯߼ᤷᴿӰᓊ䈛ṯᦤㅢ 2009/770/EC ߩ䇤∅ᒪᨆӚⴇ᧝䇗ࡈᡶࡍⲺ᧠ ᯳Ⲻᢝ㺂᛻߫ૂᇔ᯳㔉᷒Ⱦ

ㅢӊᶗ

255 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ⅝ާ։ⲱ䇦

⅝ṯᦤ⌋㿺δECεㅢ 1829/2003 ਭㅢ 28 ᶗⲺ㿺ᇐθᵢߩᇐ䱺ԬѣⲺؗᚥՐ൞ ާ։ީӄ䚍Ֆ᭯㢥伕૷ૂ侨ᯏⲺⲱ䇦ޢᐹȾ

ㅢޣᶗ

᢯߼ᤷᴿӰ

᢯߼ᤷᴿӰѰᆕኧ䜳ޢਮδMonsanto Companyθ㗄ളεⲺᆆޢਮᆕኧ䜳⅝⍨㛗 Գᴿ䲆ޢਮδMonsanto Europe S.Aθ∊࡟ᰬεȾ

ㅢйᶗ

᢯߼ᴿ᭾ᵕ

ᵢߩᇐ㠠䙐⸛ᰛᵕ䎭 10 ᒪ޻ᴿ᭾Ⱦ

ㅢޡᶗ

䙐⸛൦൶

ᵢߩᇐᇺ䘷ࡦᆕኧ䜳⅝⍨㛗Գᴿ䲆ޢਮθ൦൶ѰφAvenue de Tervuren 270-272, B-1150 Brussels — Belgium.

ᵢߩᇐӄ 2015 ᒪ 12 ᴾ 4 ᰛ൞ᐹ劷ດቊᇂᡆȾ

For the Commission Vytenis ANDRIUKAITIS Member of the Commission

䱺Ԭ㘱䈇⮛

256 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 257 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 258 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 259 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 260 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 261 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 14Ƚ᯦ࣖ඗᢯߼ᮽԬ δAVAθ伕⭞/侨⭞ε

262 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 ᯦ࣖ඗ߒѐ伕૷ф޳㦥ቶ ------᯦ࣖ඗㼋ᔀ୼ѐ॰䐥 52 ਭθ#14-01 㖇൶φhttp://www.ava.gov.sg Ỷ㍘ਭφAV 19.02.002.0005 GM

ᰛᵕφ2016 ᒪ 8 ᴾ 24 ᰛ

Kaori Murayama ᙱⴇ ᯦ࣖ඗⭩䈭ẙṾѣᗹ ᆕኧ䜳᯦ࣖ඗࠼ޢਮ⌋㿺Ӂࣗ䜞 ᯦ࣖ඗㖍ᔺ⋿ 151 ਭ ᯦〇ᢶണ॰ H ᓝ#06-08 ᯦ࣖ඗θ556741

ቀᮢⲺ Kaori ྩ༡θ

ީӄφ㓺㓺㓽㺞䗴⢯ᔸᙝᣍ㥿⭎㟜⦿㊩ MON 87427 䘑ਙ᢯߼Ⲻ⭩䈭

1. ᡇԢ൞↚ᕋ⭞ GMAC AVA 䪾ሯр䘦ᆕኧ䜳ᴿ䲆ޢਮᨆ䎭Ⲻ⭩䈭㔏࠰ⲺㆊགྷȾ 2. AVA ⌞ᝅࡦᆕኧ䜳ᴿ䲆ޢਮ䚫ᗠҼ䱺ᖋ I ѣᡶࡍᶗⅴθ䘏ӑᶗⅴᱥᡇԢҁࢃӄ 2015 ᒪ 1 ᴾ 2 ᰛᴿᶗԬ᢯߼䈛ӝ૷䘑ਙᰬ㿺ᇐⲺȾ 3. 䘏ж⅗θAVA у߃ਃሯ MON 87427 ⦿㊩㊳㋈਀ެࣖᐛӝ૷䘑ਙࡦ᯦ࣖ඗⴪᧛⭞ӄ 伕⢟ᡌ侨ᯏᡌࣖᐛ৕ᯏȾу䗽θᡶ䘦䖢ะഖӝ૷уᗍ൞᯦ࣖ඗ູ޻⭞ӄ⭦䰪⎁䈋ᡌ 㑷⇌Ⱦ ਮᴿ䍙Ա⺤ؓ઀⸛ߒ≇ᡶ〃ὃ〃ᆆѣ੡ᴿ䖢ौ։Ⲻ੃〦δྸ MONޢᆕኧ䜳ᴿ䲆 .4 87427 ⦿㊩εȾ䘏ਥԛ䙐䗽܅䖢䇟䇮ਥঅ䇤ҁ㊱ⲺᮽԬᯯᕅ䘑㺂θᶛ઀⸛ߒ≇ԌԢ ሼ᫣〃〃ᆆⲺ䓡ԳȾ ਮҕᴿ䍙Ա⺤ؓ㔝㔣䚫ᗠ䱺ᖋ I ᶗⅴδiiiεૂδivεѣ⴮ީ㿺ᇐȾޢᆕኧ䜳ᴿ䲆 .5

ᛞ䈐ᥐⲺθ

Yap Him Hoo ঐ༡ ᯦ࣖ඗ߒѐ伕૷ф޳㦥ቶቶ䮵

263 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 䱺ᖋ I

ᴿީᡶ䘦䖢ะഖ⭕⢟Ⲻ㔅ᴿ᭾ᙝ僂䇷ⲺỶ⎁ᯯ⌋Ƚ䱩ᙝḽ߼⢟䍞ૂᓅࡍؗבδiε ᨆ ᚥθ⭞ӄ AVA ൞᢯߼੄䘑㺂ⴇ⎁ૂḽ߼⢟䍞ؓᆎȾ

ᴿީᡶ䘦䖢ะഖ⭕⢟〃ὃളⲺᴶ᯦ؗᚥθԛ਀人ᵕ⭞ӄ伕⭞/侨⭞⭕ӝ⭞䙊בδiiε ᨆ Ⲻ〃ὃ䶘〥Ⱦ

ᙝ䇺ԭ㔉᷒ޞδiiiε жᰜਇ⧦ᴿԱ֋ਥ㜳ᖧଃᡶ䘦䖢ะഖ⭕⢟൞伕⢟/侨ᯏѣֵ⭞ᆿ Ⲻ᯦Ⲻ〇ᆜਇ⧦ᡌᢶᵥؗᚥθԛ਀Ա֋伕⢟/侨ᯏᣋ᭴ᐸ൰ളᇬѣ࠰⧦⾷↘ᡌ䲆 ࡬ֵ⭞Ⲻ᛻߫θ亱਀ᰬ઀⸛ AVAȾ

ᙝθᒬ䚫ᆾᡶᴿ⴮ީ⌋ᗁૂ⌋㿺㾷≸Ⱦޞδivε ⺤ؓᡶ䘦䖢ะഖ⭕⢟Ⲻᆿ

264 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 265 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 266 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 267 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 10. ⭦䰪ⴇ᧝ᯯṾθऻᤢⴇ᧝ᢶᵥȽᣍᙝ⋱⨼᧠᯳Ƚ䮵ᵕ⧥ູ᭾ᓊⲺ ⹊ガᯯ⌋ㅿ ᵜ⭣䈧Җ⢩Ѫ㧧ᗇ MON 87427 䘋ਓ⭘֌࣐ᐕ৏ᯉⲴᆹޘ䇱ҖˈнՊ൘ѝഭ ຳ޵⿽ἽǄ ྲ᷌㜭ཏ㧧ᗇ MON 87427 Ⲵ䘋ਓᆹޘ䇱Җˈᆏኡ䜭ޜਨՊ᤹➗⴨ޣⲴ⌅ᖻ 䇱Җˈᒦ᰾⺞㾱≲䍨ޘ䇱ҖкⲴ㾱≲ѕṬ֯⭘઼㇑⨶ᆹޘ㿴઼ߌъ䜘ᢩ߶Ⲵᆹ⌅ ᱃୶␵ᾊҶ䀓ᢩ߶Ⲵᆹޘ䇱Җᓄ⭘㤳ത઼ᴹ᭸ᵏˈ㾱≲䍨᱃୶൘ᴹ᭸ᵏ㤳ത޵ੁ ᆏኡ䜭ޜਨ⭣䈧 MON 87427 䘋ਓᆹޘ䇱ҖⲴ༽ঠԦǄᆏኡ䜭ޜਨሩ䍨᱃୶൘ᆹ ˖䇱Җ֯⭘кⲴ㾱≲ሶवᤜޘ 1ǃ㾱≲䍨᱃୶ᗵ享ᤱᴹ⴨ᓄ䖜ॆփⲴ䘋ਓᆹޘ䇱Җ᡽㜭䘋ਓਜ਼ᴹ⴨ᓄ䖜ॆ փⲴ୶૱Ǆ 䇱Җ༽ঠԦਚ׋⭣䈧Ⲵ䍨᱃୶㠚⭘ᡆަᤕᴹᡆ᧗ޘਨᨀ׋Ⲵᆹޜ2ǃᆏኡ䜭 ਨ֯⭘ǄޜࡦⲴᆀ ༽䇱Җˈнᗇሶަޘ䇱ҖⲴᢩ߶㤳ത֯⭘ᆹޘ3ǃ䍨᱃୶ᗵ享਼᜿ਚ᤹➗ᆹ ঠԦᨀ׋㔉ԫօㅜйᯩǄ Ⲵ⌅ᖻ઼⌅㿴Ǆޣ䇱Җ䘋㹼Ӕ᱃ᰦᗵ享ѕṬ䚥ᆸ⴨ޘ4ǃ䍨᱃୶൘֯⭘ᆹ ˈ䇱Җਚݱ䇨֌Ѫ࣐ᐕ৏ᯉ⭘Ҿ伏⭘৺侢⭘Ⲵ৏ᯉⲴ䘋ਓޘ5ǃᡰᨀ׋Ⲵᆹ н᭟ᤱ֌Ѫ䚇Ր⹄ウᡆ⿽ἽⲴᶀᯉ䘋ਓǄ䍨᱃୶ᗵ享਼᜿䟷ਆᗵ㾱ਸ⨶ 䇱䘋ਓ৏ᯉнՊ㻛䟺᭮ࡠ⧟ຳѝᡆ㘵㻛⭘Ҿਟ㜭؍᧗ࡦ᡻⇥ˈԕޘⲴᆹ ᓧᆈ൘▌൘仾䲙Ⲵ⭘䙄Ǆڕሩ⭏⢙ཊṧᙗǃਟᤱ㔝࡙⭘ᡆӪ㊫䇱Җޘ⮉᭦എᆹ؍ਨޜ䇱Җˈᆏኡ䜭ޘ6ǃྲ᷌䍨᱃୶⋑ᴹ᤹➗㿴ᇊ֯⭘ᆹ ⭘ᵳⲴᵳ࣋Ǆ֯ ਨ䘈Պ䈖㓶䇠ᖅޜ䇱Җ֯⭘кሩ䍨᱃୶ᨀࠪ᰾⺞㾱≲ཆˈᆏኡ䜭ޘ䲔Ҷ൘ᆹ ਨ਽〠ǃൠ൰ǃ㚄㌫Ӫǃޜ䇱Җ༽ঠԦⲴ䍨᱃୶ؑ᚟ˈवᤜޘᆈᡰᴹ⭣䈧䗷ᆹ؍ᒦ 䇱ҖⲴ䍨᱃୶䘋㹼ޘਨᆹޜ㚄㌫⭥䈍઼⭥ᆀ䛞㇡ൠ൰ˈԕׯᴤྭⲴሩ֯⭘ᆏኡ䜭 ⴁⶓǄ↔ཆˈᆏኡ䜭ޜਨ䘈Պቡव㻵/ḷ䇶઼䘀䗃⧟㢲ˈሩӾһ䖜สഐӗ૱䍨᱃ Ⲵ䍨᱃୶ᨀࠪ㾱≲ˈᒦнᇊᵏⲴഎ䇯䍨᱃୶ˈሩ䘋ਓӗ૱䘋㹼ⴁⶓˈྲਁ⧠ԫօ 䘍㿴᫽֌ᡆ᜿ཆһԦਁ⭏ˈ・ণੁᖃൠⲴߌъ㹼᭯ѫ㇑䜘䰘઼ߌъ䜘ᣕ੺Ǆ

268 ᆏኡ䜭ޜਨ⭣䈧Җ˄2017˅_MON 87427 11. ᇗḛᡶ䴶Ⲻެᆹ⴮ީ䍺ᯏ 䲿ᵜ⭣䈧Җа䎧ᨀӔⲴ䘈वᤜаӋޣҾ MON 87427 Ⲵᆹޘ䇴ԧ⹄ウᣕ੺Ǆ ᨀӔⲴ⹄ウᣕ੺㿱ᵜ⭣䈧ҖⲴѝ޼઼л޼Ǆ