bioRxiv preprint doi: https://doi.org/10.1101/817775; this version posted October 30, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Charrs of the genus Salvelinus (Salmonidae): hybridization, phylogeny and evolution ALEXANDER G. OSINOV1, ALEXANDER A.VOLKOV2, NIKOLAI S. MUGUE2, 1Biological Faculty, Lomonosov Moscow State University, Moscow, 119991 Russia 2Russian Federal Research Institute of Fisheries and Oceanography (VNIRO), Moscow, 107140 Russia Running heads: PHYLOGENY AND HYBRIDIZATION IN SALVELINUS Corresponding author. E-mail:
[email protected] bioRxiv preprint doi: https://doi.org/10.1101/817775; this version posted October 30, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 2 ABSTRACT Evolutionary history, systematics and taxonomy of charrs of the genus Salvelinus and especially of the representatives of the S. alpinus – S. malma species complex remain confused that is connected with a substantial ecological and morphological flexibility of this group and with supposed ancient hybridization between some taxa. For the analysis of phylogenetic relationships and introgressive hybridization between the species of the genus Salvelinus including three endemic species from Lake El’gygytgyn and all main representatives of the S. alpinus – S. malma species complex, nucleotide sequences of mtDNA control region (960 bp) and two nuclear genes (ITS1 (581 bp) and RAG1 (899 bp)) were analyzed. The differences in the topologies of individual gene trees, among others reasons, were connected with incomplete lineage sorting and historical introgressive hybridization between certain taxa.