Zeb2 Regulates Cell Fate at the Exit from Epiblast State In
View metadata, citation and similar papers at core.ac.uk EMBRYONIC STEM CELLS/INDUCEDbrought to you by CORE PLURIPOTENT STEMprovidedC byELLS Ghent University Academic Bibliography Zeb2 Regulates Cell Fate at the Exit from Epiblast State in Mouse Embryonic Stem Cells aDepartment of Development and Regeneration, KU a a,b c,d,e,f a b Leuven, Leuven 3000, AGATA STRYJEWSKA, RUBEN DRIES, TIM PIETERS, GRIET VERSTAPPEN, ANDREA CONIDI, a a a b,g Belgium; bDepartment of Cell KATHLEEN CODDENS, ANNICK FRANCIS, LIEVE UMANS, WILFRED F. J. VAN IJCKEN, g d,e h b c,d,e,i Biology, Center for Biomics, GEERT BERX, LEO A. VAN GRUNSVEN, FRANK G. GROSVELD, STEVEN GOOSSENS, c,d,i a,b Erasmus University Medical JODY J. HAIGH, DANNY HUYLEBROECK Center, Rotterdam 3015 CN, c The Netherlands; VIB Key Words. Cell differentiation • DNA-methylation • Embryonic stem cells • Pluripotent stem cells Inflammation Research • Repressors • RNA-sequencing • Transcription factors • Transcriptom Center (IRC), Unit Vascular Cell Biology, dDepartment of Biomedical Molecular Biology, eVIB-IRC, Unit ABSTRACT Molecular and Cellular In human embryonic stem cells (ESCs) the transcription factor Zeb2 regulates neuroectoderm ver- Oncology, Ghent University, sus mesendoderm formation, but it is unclear how Zeb2 affects the global transcriptional regulato- f Ghent 9052, Belgium; Center ry network in these cell-fate decisions. We generated Zeb2 knockout (KO) mouse ESCs, subjected for Medical Genetics, Ghent them as embryoid bodies (EBs) to neural and general differentiation and carried out temporal University Hospital, Ghent h RNA-sequencing (RNA-seq) and reduced representation bisulfite sequencing (RRBS) analysis in neu- 9000, Belgium; Department ral differentiation.
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