Identification and Localization of a Sea Urchin

Total Page:16

File Type:pdf, Size:1020Kb

Identification and Localization of a Sea Urchin Development 124, 3363-3374 (1997) 3363 Printed in Great Britain © The Company of Biologists Limited 1997 DEV3707 Identification and localization of a sea urchin Notch homologue: insights into vegetal plate regionalization and Notch receptor regulation David R. Sherwood and David R. McClay* Developmental, Cell and Molecular Biology Group, Box 91000, Duke University, Durham, NC 27708, USA *Author for correspondence (e-mail: dmcclay@acpub.duke.edu) SUMMARY The specifications of cell types and germ-layers that arise plate. Experimental perturbations and quantitative from the vegetal plate of the sea urchin embryo are thought analysis of LvNotch expression demonstrate that the mes- to be regulated by cell-cell interactions, the molecular basis enchyme blastula vegetal plate contains both of which are unknown. The Notch intercellular signaling animal/vegetal and dorsoventral molecular organization pathway mediates the specification of numerous cell fates even before this territory invaginates to form the archen- in both invertebrate and vertebrate development. To gain teron. Furthermore, these experiments suggest roles for the insights into mechanisms underlying the diversification of Notch pathway in secondary mesoderm and endoderm vegetal plate cell types, we have identified and made anti- lineage segregation, and in the establishment of dorsoven- bodies to a sea urchin homolog of Notch (LvNotch). We tral polarity in the endoderm. Finally, the specific and dif- show that in the early blastula embryo, LvNotch is absent ferential subcellular expression of LvNotch in apical and from the vegetal pole and concentrated in basolateral basolateral membrane domains provides compelling membranes of cells in the animal half of the embryo. evidence that changes in membrane domain localization of However, in the mesenchyme blastula embryo LvNotch LvNotch are an important aspect of Notch receptor shifts strikingly in subcellular localization into a ring of function. cells which surround the central vegetal plate. This ring of LvNotch delineates a boundary between the presumptive secondary mesoderm and presumptive endoderm, and has Keywords: sea urchin, Notch, phylogeny, vegetal plate, subcellular an asymmetric bias towards the dorsal side of the vegetal localization INTRODUCTION segregation that must occur in cells derived from the vegetal plate is the differential specification of SMCs and endoderm The vegetal plate of the sea urchin mesenchyme blastula cells. Lineage analysis has shown that the presumptive SMCs embryo is a structurally simple, yet developmentally dynamic, lie in the central region of the mesenchyme blastula vegetal region. This concave single cell-layer epithelium contains all plate and are clonally distinct from the presumptive endoderm three future germ-layers of the embryo concentrically arranged cells, which are positioned in a ring around the SMCs (Ruffins within a few cell diameters (Ruffins and Ettensohn, 1996). and Ettensohn, 1996). The clonal isolation of presumptive During gastrulation, vegetal plate cells undergo dramatic SMCs from endoderm cells is consistent with these germ- movements and shape changes to invaginate and form the layers being differentially specified before vegetal plate invagi- archenteron (reviewed in Hardin, 1996). Cells at tip of the nation. To date, however, molecular evidence has suggested archenteron segregate and become secondary mesenchyme otherwise. The vegetal plate marker Endo16 and sea urchin cells (SMCs). Behind the SMCs, the archenteron gives rise to homologs of brachyury and forkhead are expressed throughout the endoderm, which subdivides into several regions. Finally, the vegetal plate prior to invagination; only after invagination the ectoderm is excluded from the archenteron. These changes has begun do these and other identified molecular markers that occur in cells derived from the vegetal plate during gas- become differentially expressed between SMCs and endoderm trulation offer a relatively simple cellular model for studying lineages (Ransick et al., 1993; Harada et al., 1995, 1996; the integration of mechanisms underlying cell fate diversifica- reviewed in Davidson, 1993). Therefore, it remains unclear tion and morphogenesis. when these germ-layers are specified. Given the evidence for Cellular studies of vegetal plate regionalization suggest that cell-cell interactions specifying cell-types in the vegetal plate, local cell-cell interactions play an essential role in the diversi- an analysis of evolutionarily conserved intercellular signaling fication of cell-types that arise from the vegetal plate (McClay pathways may shed light into the time and mechanism by and Logan, 1996; reviewed in Davidson, 1993). The molecular which these two germ-layers become distinct. basis of these cell-cell interactions, however, is unknown. One The Notch signaling pathway mediates cell-cell interactions 3364 D. R. Sherwood and D. R. McClay leading to the specification and patterning of a wide array of LvNotch gave no evidence for differential processing of the LvNotch cell types in both invertebrate and vertebrate development gene. (Conlon et al., 1995; de Celis et al., 1996; reviewed in Artavanis-Tsakonas et al., 1995). The conserved role and Northern analysis µ + pleiotropic function of this pathway made it a likely candidate A 1.0% agarose/formaldehyde gel was loaded with 3 g/lane poly(A) for involvement in the diversification of cell-types arising from RNA (isolated with QuickPrep; Pharmacia), fractioned by elec- trophoresis, transferred onto a nylon membrane (Gene Screen, NEN the vegetal plate. The Drosophila Notch gene and two related Research Products), and hybridized with a 948 bp fragment of genes, lin-12 and glp-1 in C. elegans, have been identified as LvNotch corresponding to a portion of the extracellular domain receptors in the Notch pathway. These genes, and four identi- (amino acids 798-1114). The blot was then probed and washed as fied vertebrate homologs of Notch, all encode single-spanning described (Bachman and McClay, 1995). Identical results to those transmembrane proteins (Uyttendaele et al., 1996; reviewed in shown in Fig. 2 were also obtained with a probe corresponding to a Artavanis-Tsakonas et al., 1995). Studies on the localization of portion of the intracellular domain (data not shown). both Drosophila Notch and C. elegans GLP-1 have led to the proposal that the activity of this signaling pathway may in part Antibody production be controlled by the precise distribution of the receptor (Fehon Eight fusion proteins were expressed using subcloned or PCR- et al., 1991; Crittenden et al., 1994). Therefore, knowledge of amplified fragments of LvNotch in pGex1, 2 and 3 vectors (Glu- tathione S-transferase (GST) expression system; Smith and Johnson, the localization of Notch proteins is a likely indicator of where 1988). The fusion proteins and corresponding LvNotch amino acids this pathway functions. they encompass are as follows: Pet1, 29-287; Pet2, 198-464; 2s, 488- We have isolated and generated antibodies to the first echin- 799; Bam3, 715-878; Bam4, 1002-1130; Bam1, 1128-1472; Ank, oderm Notch homolog (LvNotch). Analysis of LvNotch 1751-2095; CloneB, 1886-2531. All fusion proteins were expressed expression demonstrates that the presumptive SMCs and and purified as described (Smith and Johnson, 1988), with the endoderm are indeed differentially specified in the mes- exception of 2s and CloneB. The fusion protein 2s was insoluble and enchyme blastula embryo, earlier than any previous markers purified as outlined for Dmoesin (McCartney and Fehon, 1996). have shown. In addition, there is a dorsal bias in the expression Isolated fusion proteins were injected into animals to generate poly- of LvNotch in the mesenchyme blastula vegetal plate that is clonal antibodies (pAb; Harlow and Lane, 1988); clone B, which maintained in the presumptive endoderm during invagination. could not be eluted from the glutathione-agarose beads, was injected coupled to these beads. Animals injected with fusion proteins were as These results, together with several experimental manipula- follows: mice, all fusion proteins; guinea pigs, Bam1 and Ank; tions and a quantitative analysis of LvNotch expression, Rabbits, Ank. Whole serum from mice was prepared by ammonium suggest that the Notch signaling pathway is involved in the seg- sulfate precipitation (Harlow and Lane, 1988). Serum from guinea regation of SMCs from endoderm cells, and in the establish- pigs and rabbits was affinity purified as described (McCartney and ment of a dorsoventral axis in the endoderm. Finally, we Fehon, 1996). Specificity of antibodies to LvNotch was determined present compelling evidence that shifts in membrane domain by staining gastrula-stage embryos and comparing immunofluores- localization are an important component of Notch receptor cence patterns. Controls for specificity included staining with pre- function. immune serum, staining of embryos with antibodies to GST, and staining after pre-incubation of pAb with the appropriate fusion protein. MATERIALS AND METHODS Western analysis Protein extracts were prepared by pelleting embryos, adding 10 µl of Animals pelleted embryos to ice-cold SDS Lysis buffer (200 µl; 100 mM Tris, Sea urchins (Lytechinus variegatus) were obtained from Susan Decker pH 6.8; 4% SDS; 20% glycerol; 1 mM PMSF; 1 µg/mL Leupeptin), (Hollywood, FL) and Tracy Andacht (Duke University Marine Labo- douncing 4× with a pestle, and then immediately boiling for 5 ratory). Gametes were harvested and cultured as described
Recommended publications
  • Divergence of Ectodermal and Mesodermal Gene Regulatory Network Linkages in Early Development of Sea Urchins
    Divergence of ectodermal and mesodermal gene regulatory network linkages in early development of sea urchins Eric M. Erkenbracka,1,2 aDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125 Edited by Neil H. Shubin, The University of Chicago, Chicago, IL, and approved October 5, 2016 (received for review August 3, 2016) Developmental gene regulatory networks (GRNs) are assemblages of to study in sea urchins, whose lineages have undergone multiple gene regulatory interactions that direct ontogeny of animal body changes in life history strategies (8). Importantly, an excellent fossil plans. Studies of GRNs operating in the early development of record affords dating of evolutionary events (9), which has established euechinoid sea urchins have revealed that little appreciable change has that the sister subclasses of sea urchins—cidaroids and euechinoids— occurred since their divergence ∼90 million years ago (mya). These diverged at least 268 million years ago (mya) (10). Differences in observations suggest that strong conservation of GRN architecture the timing of developmental events in embryogenesis of cida- was maintained in early development of the sea urchin lineage. Test- roids and euechinoids have long been a topic of interest, but ing whether this holds for all sea urchins necessitates comparative have become the subject of molecular research only recently analyses of echinoid taxa that diverged deeper in geological time. (11–18). Recent studies highlighted extensive divergence of skeletogenic me- Research on the early development of the euechinoid purple soderm specification in the sister clade of euechinoids, the cidaroids, sea urchin Strongylocentrotus purpuratus (Sp) has brought into suggesting that comparative analyses of cidaroid GRN architecture high resolution the players and molecular logic directing devel- may confer a greater understanding of the evolutionary dynamics of opmental GRNs that specify Sp’s early embryonic domains developmental GRNs.
    [Show full text]
  • Animal Form & Function
    Animals Animal Form & Function By far, most diversity of bauplane (body forms). And most variations within bauplane. Animals are Animated “ANIMAL” ≠ MAMMAL — Fascinating Behaviors Animal Cells • Eukaryotic • No cell wall No plastids No central vacuole • Multicellular: – extensive specialization & differentiation – unique cell-cell junctions Heyer 1 Animals Animals Blastulation & Gastrulation • Motile • Early embryonic development in animals 3 In most animals, cleavage results in the formation of a multicellular stage called a 1 The zygote of an animal • Highly differentiated blastula. The blastula of many animals is a undergoes a succession of mitotic tissues cell divisions called cleavage. hollow ball of cells. Blastocoel • Intercellular junctions Cleavage Cleavage – tissue-specific cadherins 6 The endoderm of the archenteron develops into Eight-cell stage Blastula Cross section Zygote • Extracellular protein the the animal’s of blastula fibers digestive tract. Blastocoel Endoderm – collagen 5 The blind pouch formed by gastrulation, called Ectoderm • Diploid life cycle the archenteron, opens to the outside Gastrula Gastrulation via the blastopore. Blastopore 4 Most animals also undergo gastrulation, a • Blastula/gastrula rearrangement of the embryo in which one end of the embryo folds inward, expands, and eventually fills the embryo blastocoel, producing layers of embryonic tissues: the Figure 32.2 ectoderm (outer layer) and the endoderm (inner layer). Primary embryonic germ layers Primary embryonic germ layers • Diploblastic: two germ
    [Show full text]
  • T-Brain Regulates Archenteron Induction Signal 5207 Range of Amplification
    Development 129, 5205-5216 (2002) 5205 Printed in Great Britain © The Company of Biologists Limited 2002 DEV5034 T-brain homologue (HpTb) is involved in the archenteron induction signals of micromere descendant cells in the sea urchin embryo Takuya Fuchikami1, Keiko Mitsunaga-Nakatsubo1, Shonan Amemiya2, Toshiya Hosomi1, Takashi Watanabe1, Daisuke Kurokawa1,*, Miho Kataoka1, Yoshito Harada3, Nori Satoh3, Shinichiro Kusunoki4, Kazuko Takata1, Taishin Shimotori1, Takashi Yamamoto1, Naoaki Sakamoto1, Hiraku Shimada1 and Koji Akasaka1,† 1Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan 2Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan 3Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan 4LSL, Nerima-ku, Tokyo 178-0061, Japan *Present address: Evolutionary Regeneration Biology Group, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan †Author for correspondence (e-mail: koji@hiroshima-u.ac.jp) Accepted 30 July 2002 SUMMARY Signals from micromere descendants play a crucial role in cells, the initial specification of primary mesenchyme cells, sea urchin development. In this study, we demonstrate that or the specification of endoderm. HpTb expression is these micromere descendants express HpTb, a T-brain controlled by nuclear localization of β-catenin, suggesting homolog of Hemicentrotus pulcherrimus. HpTb is expressed that
    [Show full text]
  • Apoptosis in Amphibian Development
    Advances in Bioscience and Biotechnology, 2012, 3, 669-678 ABB http://dx.doi.org/10.4236/abb.2012.326087 Published Online October 2012 (http://www.SciRP.org/journal/abb/) Apoptosis in amphibian development Jean-Marie Exbrayat, Elara N. Moudilou, Lucie Abrouk, Claire Brun Université de Lyon, UMRS 449, Biologie Générale, Université Catholique de Lyon, Reproduction et Développement Comparé, Ecole Pratique des Hautes Etudes, Lyon, France Email: jmexbrayat@univ-catholyon.fr Received 13 August 2012; revised 20 September 2012; accepted 28 September 2012 ABSTRACT divide to give the first two blastomeres which continue to divide becoming a morula. An inner cavity, the blasto- Amphibians and more particularly X. laevis are mod- coel, appears in the mass cell of the embryo, becoming a els often used for studying apoptosis during embry- blastula. During this period of cleavage, the size and onic development. Using several methods, searchers shape of embryos do not vary. Before mid blastula tran- determined the localization of programmed cell sition (MBT), zygotic genes do not express excepted deaths (PCD). Several experimental methods also those encoding for proteins implicated in membrane have been used to understand the regulatory mecha- building. Maternal mRNAs previously accumulated in nisms of apoptosis, throughout development, contrib- the oocytes remain present in the cytoplasm of zygote uting to elucidate the general action of several genes and they are distributed into the cytoplasm of blas- and proteins. Apoptosis occurs very early, with a first tomeres during cleavage. These maternal mRNAs encode program under control of maternal genes expressed for proteins which will be implicated in expression of before MBT, in order to eliminate damaged cells be- zygotic genes.
    [Show full text]
  • Archenteron Precursor Cells Can Organize Secondary Axial Structures in the Sea Urchin Embryo
    Development 124, 3461-3470 (1997) 3461 Printed in Great Britain © The Company of Biologists Limited 1997 DEV3652 Archenteron precursor cells can organize secondary axial structures in the sea urchin embryo Hélène Benink1, Gregory Wray2 and Jeff Hardin1,3,* 1Department of Zoology and 3Program in Cell and Molecular Biology, University of Wisconsin, Madison, 1117 West Johnson Street, Madison, WI 53706, USA 2Department of Ecology and Evolution, SUNY, Stony Brook, NY 11794, USA *Author for correspondence: (e-mail: jdhardin@facstaff.wisc.edu) SUMMARY Local cell-cell signals play a crucial role in establishing terning sites within the ectoderm. The ectopic skeletal major tissue territories in early embryos. The sea urchin elements are bilaterally symmetric, and flank the ectopic embryo is a useful model system for studying these inter- archenteron, in some cases resulting in mirror-image, actions in deuterostomes. Previous studies showed that symmetric skeletal elements. Since the induced patterned ectopically implanted micromeres from the 16-cell embryo ectoderm and supernumerary skeletal elements are derived can induce ectopic guts and additional skeletal elements in from the host, the ectopic presumptive archenteron tissue sea urchin embryos. Using a chimeric embryo approach, we can act to ‘organize’ ectopic axial structures in the sea show that implanted archenteron precursors differentiate urchin embryo. autonomously to produce a correctly proportioned and patterned gut. In addition, the ectopically implanted pre- sumptive archenteron tissue induces ectopic skeletal pat- Key words: induction, gastrulation, sea urchin, endoderm INTRODUCTION mapping studies have shown that veg1 descendants contribute tissue to the vegetal regions of the archenteron by the late Progressive refinement of the embryonic body plan via local gastrula stage (Logan and McClay, 1997); by this time veg1 inductive interactions is a common theme among animal and veg2 descendants intermingle within the wall of the archen- embryos.
    [Show full text]
  • Regulative Capacity of the Archenteron During Gastrulation in the Sea Urchin
    Development 122, 607-616 (1996) 607 Printed in Great Britain © The Company of Biologists Limited 1996 DEV3313 Regulative capacity of the archenteron during gastrulation in the sea urchin David R. McClay* and Catriona Y. Logan Developmental, Cellular and Molecular Biology Group, LSRC, Duke University, Durham NC 27708, USA *Author for correspondence (e-mail: dmcclay@acpub.duke.edu) SUMMARY Gastrulation in the sea urchin involves an extensive ognizable morphology of the archenteron was re-estab- rearrangement of cells of the archenteron giving rise to lished. Long after the archenteron reveals territorial speci- secondary mesenchyme at the archenteron tip followed by fication through expression of specific markers, the endo- the foregut, midgut and hindgut. To examine the regula- dermal cells remain capable of being respecified to other tive capacity of this structure, pieces of the archenteron gut regions. Thus, for much of gastrulation, the gut is con- were removed or transplanted at different stages of gas- ditionally specified. We propose that this regulative ability trulation. After removal of any or all parts of the archen- requires extensive and continuous short-range communi- teron, the remaining veg 1 and/or veg 2 tissue regulated to cation between cells of the archenteron in order to reor- replace the missing parts. Endoderm transplanted to ganize the tissues and position the boundaries of this ectopic positions also regulated to that new position in the structure even after experimental alterations. archenteron. This ability to replace or regulate endoderm did not decline until after full elongation of the archenteron was completed. When replacement occurred, the new gut Key words: morphogenesis, cell lineage, cell fate, differentiation, was smaller relative to the remaining embryo but the rec- specification.
    [Show full text]
  • Chapter 4 Sea Urchin Development Carolyn M
    Chapter 4 Sea Urchin Development Carolyn M. Conway Don Igelsrud Department of Biology Department of Biology Virginia Commonwealth University The University of Calgary Richmond, Virginia 23284 USA Calgary, Alberta T2N 1N4 Canada Arthur F. Conway Department of Biology Randolph-Macon College Ashland, Virginia 23005 USA Carolyn M. Conway is currently an Assistant Professor of Biology at Virginia Com- monwealth University where she teaches Animal Embryology, Devetopmental Biology, and Teratology. She received her BS and MA degrees at Longwood College and the College of William and Mary respectively. She received her PhD from the University of Miami where her research involved an ultrastructural study of sea urchin eggs. While completing her doctorate she spent several Summers at the Marine Biological Labo- ratory as a participant in the Fertilization and Gamete Physiology Training Program. Prior to joining the VCU faculty she taught at Iowa State University and Hobart and William Smith Colleges. Her current research involves the relationship between ma- ternal autoimmune disease and birth defects. Don Igelsrud is currently an Instructor of Biology at The University of Calgary where he is in charge of the introductory zoology laboratories. He received his BS degree from the University of Kansas and his MA degree from Washington University. Prior to joining the faculty at The University of Calgary he taught at Delaware Valley College where he developed a sfrong interest in laboratory teaching, and at Northwestern Uni- versity where he was Biology Laboratory Director. His main interests are in increasing awareness and understanding of living phenomena and in finding living organisms that survive well in the laboratory with a minimum of maintenance.
    [Show full text]
  • Dynamics of Thin Filopodia During Sea Urchin Gastrulation
    Development 121, 2501-2511 (1995) 2501 Printed in Great Britain © The Company of Biologists Limited 1995 Dynamics of thin filopodia during sea urchin gastrulation Jeffrey Miller1, Scott E. Fraser2 and David McClay1,* 1Developmental, Cell and Molecular Biology, Duke University, SRC, Box 91000, Durham, NC 27708, USA 2Division of Biology, Beckman Institute (139-74), California Institute of Technology, Pasadena CA 91125, USA *Author for correspondence: e-mail dmcclay@acpub.duke.edu SUMMARY At gastrulation in the sea urchin embryo, a dramatic involvement in cell-cell interactions associated with rearrangement of cells establishes the three germ layers of signaling and patterning at gastrulation. Nickel-treatment, the organism. Experiments have revealed a number of cell which is known to create a patterning defect in skeleto- interactions at this stage that transfer patterning informa- genesis due to alterations in the ectoderm, alters the normal tion from cell to cell. Of particular significance, primary position-dependent differences in the thin filopodia. The mesenchyme cells, which are responsible for production of effect is present in recombinant embryos in which the the embryonic skeleton, have been shown to obtain ectoderm alone was treated with nickel, and is absent in extensive positional information from the embryonic recombinant embryos in which only the primary mes- ectoderm. In the present study, high resolution Nomarski enchyme cells were treated, suggesting that the filopodial imaging reveals the presence of very thin filopodia (0.2-0.4 length is substratum dependent rather than being primary µm in diameter) extending from primary mesenchyme cells mesenchyme cell autonomous. The thin filopodia provide a as well as from ectodermal and secondary mesenchyme means by which cells can contact others several cell cells.
    [Show full text]
  • 2. Archenteron Morphogenesis in the Sea Urchin David R
    Cell-Cell Interactions in Early Development, pages 15-29 © 1991 Wiley-Liss, Inc. I .f 2. Archenteron Morphogenesis in the Sea Urchin David R. McClay, John Morrill, and Jeff Hardin Department of Zoology, Duke University, Durham, North Carolina 27707 (D .R.M., J.H.); Department of Biology, New College, Sarasota, Florida 33580 (J .M.) INTRODUCTION The progression of development involves an impressive array of morphoge­ netic rearrangements, each of which involves the coordination of multiple cel­ lular functions and molecular events. We have been studying gastrulation in the sea urchin embryo as a relatively simple model system in an attempt to understand the sorts of rules observed by an embryo as it performs a single morphogenetic event. To the observer, gastrulation in this embryo involves two major cell movements. First, primary mesenchyme cells ingress and dis­ playa series of migratory behaviors leading to the assembly of the larval skel­ eton. Second, invagination of the archenteron leads to the formation of the primitive gut. The ingression and subsequent behavior of primary mesenchyme cells is examined elsewhere in this volume (Ettensohn, Chapter 11). Here we review events associated with formation of the archenteron . In echinoderm embryos, archenteron formation begins with an indentation of the vegetal plate, followed by elongation of the indented area until a tubular archenteron forms that extends into the blastocoel . Elongation continues until the tube reaches a defined, anatomically specific region on the wall of the blastocoel (Fig . I). All through invagination, secondary mesenchyme cells at the tip of the archenteron extend filopodia that make contact with the wall of the blastocoel.
    [Show full text]
  • Animal Development Bio 1413: General Zoology Laboratory Ziser, 2008 All Living Organisms Exhibit Some Form of Growth and Development
    Animal Development Bio 1413: General Zoology Laboratory Ziser, 2008 All living organisms exhibit some form of growth and development. Members of the animal kingdom have the most complex developmental cycle of any living organism. The sequence of discrete, recognizable stages that these organism pass through as they develop from the formation of a zygote (the fertilized egg) to the sexually mature adult are referred to as its developmental cycle.Animal development can be subdivided into several sequential processes: gametogenesis, fertilization, embryonic development and post embryonic development. Embryonic development includes the processes of growth, determination, differentiation and morphogenesis. 1. Gametes. The gametes are produced by the process of meiosis which differs from mitosis in that only one of each chromosome ends up in the cells after division. The male gamete, the sperm, is small and almost always flagellated. The female gamete us usually large since it contains yolk, and spherical. slides: sperm smear starfish unfertilized eggs Activity Be able to distinguish between sperm and eggs and to find the following structures on slides and illustrations: for sperm identify: head, middle piece, tail (flagellum) for egg identify: cell membrane, nucleus, nucleolus 2. Fertilization. At fertilization only a single sperm penetrates and adds its chromosomes to those in the egg. The fertilized egg then has a pair of each chromosomes, one each from the male parent and the other of each from the female parent. To prevent additional sperm from penetrating the egg a fertilization cone is produced to produce the original sperm into the egg quickly. Then a fertilization membrane expands around the egg and pushes away and “locks out” other sperm cells.
    [Show full text]
  • Amphibian Gastrulation: History and Evolution of a 125 Year-Old Concept
    Int. J. Dev. Biol. 45: 771-795 (2001) Essay Amphibian gastrulation: history and evolution of a 125 year-old concept JEAN-CLAUDE BEETSCHEN* Centre de Biologie du Développement, Université Paul-Sabatier, Toulouse, France CONTENTS I. Depicting the Amphibian gastrula: how it forms and how it changes The unnamed gastrula The gastrula concept (Haeckel, 1872) Gastrulation and formation of germ layers Blastopore formation and evolution Is there a primitive streak in Amphibians? First evidence for morphogenetic movements (Kopsch, 1895) Studies on gastrulation at the beginning of the 20th century Bottle cells: the case for cell movement Analysis of gastrulation and morphogenetic movements by means of vital staining: Vogt (1929) and his followers II. An overview of modern advances in gastrulation studies Xenopus gastrulation Urodele gastrulation Analysis of mesoderm cell migration and of gastrulation movements Bottle cells: origin and functions Are epibolic and inward movements independent of each other? Conclusions Summary The classical characteristics of a gastrula stage in Metazoan about the processes leading from a relatively simple blastula to the development were established by Haeckel (1874, 1875) and since formation of an advanced gastrula ? Its architecture is now much then have been constantly used by embryologists. Different kinds more complex, with three germ layers subdivided into several of gastrulae have been recognized in animal groups and invagina- domains that are distributed along anteroposterior and dorsoven- tion phenomena, leading
    [Show full text]
  • 409 Developmental Biology and Immunology Topic: Gastrulation Dr.Tabrez Ahmad
    M.Sc.Semester III Paper -409 Developmental Biology and Immunology Topic: Gastrulation Dr.Tabrez Ahmad • The most characteristic event occurring during the third week of gestation is gastrulation. • Gastrulation is a formative process by which the three germ layers, which are precursors of all embryonic tissues, and the axial orientation, are established in embryos. • During gastrulation, the bilaminar embryonic disc is converted into a trilaminar embryonic disc. • Extensive cell shape changes, rearrangement, movement, and alterations in adhesive properties contribute to the process of gastrulation. • The embryo during this stage is called a gastrula. Process of Gastrulation • Gastrulation begins with the formation of the primitive streak on the surface of the epiblast. • Initially, the streak is vaguely defined, but in a 15- to a 16-day embryo, it is clearly visible as a narrow groove with slightly bulging regions on either side. • The cephalic end of the streak, the primitive node, consists of a slightly elevated area surrounding the small primitive pit. • Cells of the epiblast migrate toward the primitive streak. • Upon arrival in the region of the streak, they become fl ask-shaped, detach from the epiblast, and slip beneath it. This inward movement is known as invagination. • Once the cells have invaginated, some displace the hypoblast, creating the embryonic endoderm, and others come to lie between the epiblast and newly created endoderm to form mesoderm. • Cells remaining in the epiblast then form ectoderm. • Thus, the epiblast, through the process of gastrulation, is the source of all of the germ layers, and cells in these layers will give rise to all of the tissues and organs in the embryo.
    [Show full text]